Tea oil burn and scald dressing and preparation method thereof

Abstract

The invention provides a tea oil burn and scald dressing and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine products. The tea oil burn and scald dressing is prepared from 30-40 parts of tea oil, 5-10 parts of traditional Chinese medicine extract, 5-10 parts of chitosan quaternary ammonium salt and 2-5 parts of calcium alginate, the traditional Chinese medicine extract comprises xylaria nigripes extract, ampelopsis japonica extract and mint extract. The tea oil provides a moisturizing and bacteriostatic basis, the traditional Chinese medicine extract promotes wound repair through the xylaria nigripes extract, the ampelopsis japonica extract clears away heat and toxic materials, the mint extract relieves pain, and the chitosan quaternary ammonium salt and calcium alginate form an antibacterial barrier and promote hemostasis. The tea oil burn and scald dressing has the effects of clearing away heat and toxic materials, relieving swelling and pain, resisting bacteria and stopping bleeding and promoting wound healing. Test results show that the burn and scald dressing has an excellent antibacterial effect, and the healing time of a scald wound can be remarkably shortened.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine products, and in particular to a tea oil burn and scald dressing and a preparation method thereof. Background Art

[0002] Burns and scalds are common traumatic injuries in clinical practice, resulting in a significant number of burns and scald injuries worldwide each year due to various accidents, disasters, and everyday incidents. Burns and scalds not only cause severe physical pain but can also lead to serious complications such as infection and shock, significantly impacting patients' health and quality of life. Choosing the right dressing is crucial in the treatment of burns and scalds.

[0003] Currently, the main dressings used are gauze dressings and chemically synthesized dressings. Gauze dressings have a high porosity, making them susceptible to bacterial growth; they adhere poorly to the wound surface, and frequent changes can lead to secondary damage. Some materials in chemically synthesized dressings (such as lead-containing myrrh) pose toxicity risks and lack breathability, hindering burn recovery. Most burn ointments are based on single ingredients such as tea oil, honey, and petrolatum. While they have anti-inflammatory properties, they lack the ability to sustainably release their active ingredients. Furthermore, most Chinese herbal preparations are crudely prepared, resulting in low extraction rates of active ingredients, making it difficult to ensure stable efficacy.

[0004] Tea oil, a traditional medicinal and edible resource, possesses significant anti-inflammatory, antioxidant, and permeation-enhancing properties, reducing oxidative stress damage to wound cells. However, research into using tea oil as a primary ingredient in burn and scald dressings is still in its infancy. Developing high-performance, safe, and effective burn and scald dressings by fully leveraging its benefits and combining it with other suitable materials through scientific preparation methods remains an urgent challenge. Summary of the Invention

[0005] The purpose of the present invention is to provide a tea oil burn and scald dressing and a preparation method thereof. The burn and scald dressing provided by the present invention has good antibacterial properties and can quickly promote the healing of burn wounds.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions: The invention provides a tea oil burn and scald dressing, comprising the following raw materials in parts by weight: 30-40 parts of tea oil, 5-10 parts of traditional Chinese medicine extracts, 5-10 parts of chitosan quaternary ammonium salt and 2-5 parts of calcium alginate; the traditional Chinese medicine extracts include radix scutellariae extract, ampelopsis pilosula extract and mint extract.

[0007] Preferably, the tea oil is obtained by supercritical CO2 extraction from tea seeds.

[0008] Preferably, the Wulingshen extract is obtained by fermenting Wulingshen sclerotia with composite microorganisms.

[0009] Preferably, the Bletilla striata extract is obtained by ultrasonically extracting Bletilla striata with an ethanol solution and purifying it with a macroporous adsorption resin.

[0010] Preferably, the mint extract is obtained by steam distillation of mint leaves.

[0011] More preferably, the weight ratio of the Wulingshen extract, the Bletilla striata extract and the Mentha arvense extract is (3-5):(1-3):(0.5-1.5).

[0012] The present invention also provides a preparation method of the tea oil burn and scald dressing, comprising: dissolving chitosan quaternary ammonium salt in deionized water to obtain a chitosan quaternary ammonium salt solution; mixing the tea oil and the chitosan quaternary ammonium salt solution, sequentially adding calcium alginate and a traditional Chinese medicine extract, homogenizing, adjusting the pH value, and sterilizing to obtain the burn and scald dressing.

[0013] Preferably, the homogenization speed is 800-1200 rpm, and the time is 15-20 min.

[0014] Preferably, the pH value is 6-6.5.

[0015] The present invention also provides application of the tea oil burn and scald dressing in preparing burn and scald medicines.

[0016] Compared with the prior art, the present invention has the following beneficial effects: The present invention provides a tea oil burn and scald dressing, comprising: 30-40 parts tea oil, 5-10 parts traditional Chinese medicine extracts, 5-10 parts chitosan quaternary ammonium salt, and 2-5 parts calcium alginate; the traditional Chinese medicine extracts include radix ulmifolia extract, radix scutellariae extract, and mint extract. The tea oil provides a moisturizing and antibacterial foundation, while the traditional Chinese medicine extracts promote wound repair through radix ulmifolia, clear heat and detoxify through radix scutellariae, and relieve pain through mint. The chitosan quaternary ammonium salt and calcium alginate form an antibacterial barrier and promote hemostasis. The tea oil burn and scald dressing has the effects of clearing heat and detoxifying, reducing swelling and relieving pain, achieving antibacterial hemostasis, and promoting wound healing.

[0017] The test results show that the burn and scald dressing of the present invention has excellent antibacterial effects on Escherichia coli, Staphylococcus aureus and Candida albicans; the results of rat experiments show that the tea oil burn and scald dressing of the present invention can significantly shorten the healing time of burn wounds. DETAILED DESCRIPTION

[0018] The invention provides a tea oil burn and scald dressing, comprising the following raw materials in parts by weight: 30-40 parts of tea oil, 5-10 parts of traditional Chinese medicine extracts, 5-10 parts of chitosan quaternary ammonium salt and 2-5 parts of calcium alginate; the traditional Chinese medicine extracts include radix scutellariae extract, ampelopsis pilosula extract and mint extract.

[0019] The tea oil of the present invention is preferably obtained from tea seeds via supercritical CO2 extraction. The tea oil extraction method preferably comprises: drying the tea seed powder to a moisture content of 2%-5% (w / w), crushing it through an 80-150 mesh sieve, and subjecting it to supercritical CO2 extraction for 1.5-2.5 hours at an extraction pressure of 25-35 MPa, a temperature of 40-45°C, and a CO2 flow rate of 15-25 L / h. The separation pressure is then adjusted to 10-15 MPa to obtain the tea oil. More preferably, the extraction parameters are: an extraction pressure of 30 MPa, a temperature of 42°C, a flow rate of 20 L / h, a time of 2 hours, and a separation pressure of 12 MPa.

[0020] The tea oil of the present invention serves as a formula matrix, provides an oily environment, helps other ingredients to be evenly dispersed and prolongs the contact time between the dressing and the wound surface. In addition, the tea oil contains a variety of anti-inflammatory ingredients, which can reduce inflammatory reactions and promote epidermal cell regeneration.

[0021] The Wuling ginseng extract of the present invention is obtained by fermenting Wuling ginseng sclerotia with composite microorganisms. The extraction method of the Wuling ginseng extract preferably includes: crushing the Wuling ginseng sclerotia through a 100-200 mesh sieve, adding deionized water according to a material-liquid ratio of 1:15-20g / mL, inoculating 3%-8% (w / w) composite microorganisms, and anaerobically fermenting for 24-36 hours at 35-40°C and a pH value of 6-6.5, filtering and sterilizing, concentrating the fermentation liquid, and drying to obtain the Wuling ginseng extract. The composite microorganism is composed of Clostridium tyrobutyricum and Lactobacillus plantarum subspecies in a mass ratio of 2-5:1-3, wherein Clostridium tyrobutyricum is Clostridium tyrobutyricum CICC ® 24853, which is sourced from the China Industrial Microorganism Culture Collection Administration Center, has a bacterial activity of preferably 40,000-80,000 CFU / g, more preferably 50,000 CFU / g; Lactobacillus plantarum subspecies plantarum is sourced from the ATCC Culture Collection of the United States, with the deposit number being ATCC14917, has a bacterial activity of preferably 100,000-150,000 CFU / g, more preferably 120,000 CFU / g; the solid-liquid ratio is more preferably 1:18 g / mL, the inoculation amount is more preferably 5%, the temperature is more preferably 37° C., the pH value is more preferably 6.3, and the time is more preferably 30 h.

[0022] The black ginseng extract of the present invention is rich in polysaccharides, saponins, polypeptides, amino acids and other ingredients, which can enhance local immunity of the wound, inhibit the risk of infection, accelerate capillary formation and collagen deposition in the wound, and promote wound healing.

[0023] The Bletilla striata extract of the present invention is preferably obtained by ultrasonic extraction of Bletilla striata with an ethanol solution and purification with a macroporous adsorption resin. The extraction method of the Bletilla striata extract preferably comprises: crushing the Bletilla striata through a 500-600 mesh sieve, adding 70%-80% (v / v) ethanol solution at a solid-liquid ratio of 1:6-13 g / mL, extracting at 65-75°C for 25-35 minutes, filtering, adsorbing the filtrate with a macroporous adsorption resin, gradient eluting with a 50%-68% (v / v) ethanol solution, collecting the eluate, concentrating, and drying to obtain the Bletilla striata extract. The material-liquid ratio is more preferably 1:10 g / mL, the ethanol solution concentration is more preferably 75% (v / v), the temperature is more preferably 70°C, the time is more preferably 30 min, the macroporous adsorption resin is preferably an AB-8 macroporous adsorption resin column, and the gradient elution preferably comprises eluting with 3-6 column volumes of 50%-54% (v / v) ethanol solution and 10-12 column volumes of 62%-65% (v / v) ethanol solution in sequence, and collecting the 62%-65% (v / v) ethanol solution eluate.

[0024] The white ampelopsis extract of the present invention clears away heat and detoxifies, can inhibit common burn pathogens such as Staphylococcus aureus, astringe and stop bleeding, reduce wound exudate, relieve swelling, eliminate inflammation, and promote wound healing.

[0025] The mint extract of the present invention is obtained by steam distillation of mint leaves. The extraction method of the mint extract preferably comprises: crushing the mint leaves through a 200-300 mesh sieve, adding deionized water at a solid-liquid ratio of 1:5-10 g / mL, ultrasonically treating the mint leaves at 30-40 kHz and 200-300 W for 20-30 minutes, and steam distilling the mint leaves at 110-120° C. for 2-4 hours to obtain the mint extract.

[0026] The mint extract of the invention promotes the penetration of the black ginseng extract and the white ampelopsis extract into the burn wound surface, thereby improving bioavailability; and when applied to the wound surface, it has a cooling effect, alleviates the burning pain of the burn wound surface, and has antibacterial and anti-inflammatory effects.

[0027] The weight ratio of the Wuling ginseng extract, Bletilla striata extract, and Mentha arvense extract described herein is preferably (3-5):(1-3):(0.5-1.5), and more preferably 4:2:1. The herbal extract of the present invention forms a functional closed loop of "antibacterial (Bletilla striata) - repair (Wuling ginseng) - analgesic (Mentha arvense)", synergizing with tea oil to reduce oxidative stress levels on wound surfaces and promote healing of burn wounds.

[0028] Calcium alginate and chitosan quaternary ammonium salt form a composite gel through positive and negative charge crosslinking, improving the mechanical properties of the dressing and enhancing its ability to bind to the skin. It can also encapsulate Chinese herbal extracts in a reticular structure, producing a sustained-release effect and increasing the dressing's antibacterial and anti-inflammatory effects and durability. Calcium ions and free fatty acids in tea oil can generate saponified products, enhancing emulsification stability. Tea oil, calcium alginate, and chitosan quaternary ammonium salt can form a semi-enclosed environment, balancing air permeability and barrier protection. The tea oil burn and scald dressing of the present invention has the effects of clearing away heat and detoxifying, reducing swelling and relieving pain, achieving antibacterial hemostasis, and promoting wound healing.

[0029] The present invention also provides a preparation method of the tea oil burn and scald dressing, comprising: dissolving chitosan quaternary ammonium salt in deionized water to obtain a chitosan quaternary ammonium salt solution; mixing the tea oil and the chitosan quaternary ammonium salt solution, sequentially adding calcium alginate and a traditional Chinese medicine extract, homogenizing, adjusting the pH value, and sterilizing to obtain the burn and scald dressing.

[0030] The homogenization speed of the present invention is preferably 800-1200 rpm, more preferably 1000 rpm, and the homogenization time is preferably 15-20 min, more preferably 18 min.

[0031] The pH value of the present invention is preferably 6-6.5, more preferably 6.2.

[0032] The present invention also provides application of the tea oil burn and scald dressing in preparing burn and scald medicines.

[0033] The method for using the tea oil burn and scald dressing is as follows: immediately after a burn, the wound surface is fully rinsed with a large amount of cold water, dirt on the wound surface is cleaned, and the tea oil burn and scald dressing is evenly applied to the wound surface, and the dressing is changed every three days.

[0034] In the present invention, unless otherwise specified, all raw material components are commercially available products well known to those skilled in the art.

[0035] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions of the present invention. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0036] In the following examples, unless otherwise specified, all methods are conventional.

[0037] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0038] Chitosan quaternary ammonium salt, also known as hydroxypropyltrimethylammonium chloride chitosan, has a degree of substitution greater than 90% and a molecular weight of 100,000 KDa and was purchased from Shanxi Lanyuan Biotechnology Co., Ltd., item number LYSW-0703-023; calcium alginate, molecular weight 1170.93, was purchased from Jiangxi Ruiweier Biotechnology Co., Ltd.; Clostridium tyrobutyricum CICC ® 24853 and Lactobacillus delbrueckii CICC ® 6289 was purchased from the China Industrial Microorganism Culture Collection Center, and Lactobacillus plantarum subsp. plantarum ATCC 14917 and Bifidobacterium bifidum ATCC 29521 were from the ATCC Culture Collection in the United States.

[0039] Example 1 Tea oil burn dressing (1) Preparation of tea oil The camellia seed powder was dried to a moisture content of 3% (w / w), crushed through a 100-mesh sieve, and subjected to supercritical extraction at an extraction pressure of 30 MPa, a temperature of 42°C, and a CO2 flow rate of 20 L / h for 2 h. The separation pressure was adjusted to 12 MPa to obtain camellia oil. (2) Preparation of Wulingshen Extract The sclerotia of Wuling ginseng were crushed and passed through a 150-mesh sieve. Deionized water was added at a solid-liquid ratio of 1:18 g / mL, and 5% (w / w) composite microorganisms were inoculated. After anaerobically fermented at 37°C and pH 6.3 for 30 h, the mixture was filtered and sterilized. The fermentation broth was concentrated and dried to a water content of 3% (w / w) to obtain a Wuling ginseng extract. Among them, the composite microorganisms consist of Clostridium tyrobutyricum CICC ® 24853 (50,000 CFU / g) and Lactobacillus plantarum subsp. plantarum ATCC14917 (120,000 CFU / g) in a mass ratio of 3:2; (3) Preparation of Bletilla striata extract The herb was crushed and passed through a 550-mesh sieve. A 75% (v / v) ethanol solution was added at a solid-liquid ratio of 1:10 g / mL. The mixture was extracted at 70°C for 30 min, filtered, and the filtrate was adsorbed on an AB-8 macroporous adsorption resin. The mixture was eluted with 5 column volumes of 52% (v / v) ethanol solution and 11 column volumes of 63% (v / v) ethanol solution, and the eluate with 63% (v / v) ethanol solution was collected, concentrated, and dried to a water content of 4% (w / w) to obtain a herb extract. (4) Peppermint extract The mint leaves were crushed through a 250-mesh sieve, deionized water was added at a solid-liquid ratio of 1:8 g / mL, and the mixture was ultrasonically treated at 35 kHz and 250 W for 25 min. The mixture was then steam distilled at 115°C for 3 h to obtain the mint extract. (5) Preparation of tea oil burn dressing Mixing the Wulingshen extract, the Bletilla striata extract, and the peppermint extract in a weight ratio of 4:2:1 to obtain a traditional Chinese medicine extract; Weigh 35 parts of tea oil, 8 parts of Chinese herbal extract, 8 parts of chitosan quaternary ammonium salt and 3 parts of calcium alginate according to weight; Chitosan quaternary ammonium salt is stirred with 8 times its weight of deionized water at 35°C and 300 rpm for 30 minutes to obtain a chitosan quaternary ammonium salt solution; tea oil is slowly added to the chitosan quaternary ammonium salt solution, stirred at 800 rpm for 25 minutes, calcium alginate and traditional Chinese medicine extract are added, homogenized at 1000 rpm for 18 minutes, and the pH value is adjusted to 6.2 to obtain a tea oil burn and scald dressing.

[0040] Example 2 Tea oil burn dressing (1) Preparation of tea oil The camellia seed powder was dried to a moisture content of 2% (w / w), crushed through an 80-mesh sieve, and subjected to supercritical extraction for 2.5 h at an extraction pressure of 25 MPa, a temperature of 40°C, and a CO2 flow rate of 15 L / h. The separation pressure was adjusted to 10 MPa to obtain camellia oil. (2) Preparation of Wulingshen Extract The sclerotia of the black ginseng were crushed and passed through a 100-mesh sieve. Deionized water was added at a solid-liquid ratio of 1:15 g / mL, and 8% (w / w) composite microorganisms were inoculated. After anaerobically fermented at 35°C and pH 6 for 24 hours, the mixture was filtered and sterilized. The fermentation broth was concentrated and dried to a water content of 4% (w / w) to obtain the black ginseng extract. Among them, the composite microorganisms consist of Clostridium tyrobutyricum CICC ® 24853 (active 40,000 CFU / g) and Lactobacillus plantarum subsp. plantarum ATCC14917 (active 100,000 CFU / g) in a mass ratio of 2:1; (3) Preparation of Bletilla striata extract The herb was crushed and passed through a 500-mesh sieve. 80% (v / v) ethanol solution was added at a solid-liquid ratio of 1:6 g / mL. The mixture was extracted at 65°C for 35 min, filtered, and the filtrate was adsorbed on an AB-8 macroporous adsorption resin. The mixture was eluted with 3 column volumes of 50% (v / v) ethanol solution and 10 column volumes of 62% (v / v) ethanol solution, and the eluate with 62% (v / v) ethanol solution was collected, concentrated, and dried to a water content of 3% (w / w) to obtain a herb extract. (4) Peppermint extract The mint leaves were crushed through a 200-mesh sieve, deionized water was added at a solid-liquid ratio of 1:5 g / mL, and ultrasonic treatment was performed at 30 kHz and 200 W for 30 min. The resulting distillate was steam distilled at 110°C for 4 h to obtain the mint extract. (5) Preparation of tea oil burn dressing Mixing the Wulingshen extract, the Bletilla striata extract, and the peppermint extract in a weight ratio of 3:1:0.5 to obtain a traditional Chinese medicine extract; Weigh 30 parts of tea oil, 5 parts of Chinese herbal extract, 5 parts of chitosan quaternary ammonium salt and 2 parts of calcium alginate according to weight; Chitosan quaternary ammonium salt is stirred with 6 times its weight of deionized water at 30°C and 200 rpm for 40 minutes to obtain a chitosan quaternary ammonium salt solution; tea oil is slowly added to the chitosan quaternary ammonium salt solution, stirred at 700 rpm for 30 minutes, calcium alginate and traditional Chinese medicine extract are added, homogenized at 800 rpm for 20 minutes, and the pH value is adjusted to 6 to obtain a tea oil burn and scald dressing.

[0041] Example 3 Tea oil burn dressing (1) Preparation of tea oil The camellia seed powder was dried to a moisture content of 4% (w / w), crushed to pass through a 150-mesh sieve, and subjected to supercritical extraction for 1.5 h at an extraction pressure of 35 MPa, a temperature of 45°C, and a CO2 flow rate of 25 L / h. The separation pressure was adjusted to 15 MPa to obtain camellia oil. (2) Preparation of Wulingshen Extract The sclerotia of the black ginseng were crushed and passed through a 200-mesh sieve. Deionized water was added at a solid-liquid ratio of 1:20 g / mL, and 3% (w / w) composite microorganisms were inoculated. After anaerobically fermented at 40°C and pH 6.5 for 36 hours, the mixture was filtered and sterilized. The fermentation broth was concentrated and dried to a water content of 5% (w / w) to obtain the black ginseng extract. Among them, the composite microorganisms consist of Clostridium tyrobutyricum CICC ® 24853 (80,000 CFU / g) and Lactobacillus plantarum subsp. plantarum ATCC14917 (150,000 CFU / g) in a mass ratio of 5:3; (3) Preparation of Bletilla striata extract The herb was crushed and passed through a 600-mesh sieve. A 70% (v / v) ethanol solution was added at a solid-liquid ratio of 1:13 g / mL. The mixture was extracted at 75°C for 25 min, filtered, and the filtrate was adsorbed on an AB-8 macroporous adsorption resin. The mixture was eluted with 6 column volumes of 54% (v / v) ethanol solution and 12 column volumes of 65% (v / v) ethanol solution, and the eluate with 65% (v / v) ethanol solution was collected, concentrated, and dried to a water content of 2% (w / w) to obtain a herb extract. (4) Peppermint extract The mint leaves were crushed through a 300-mesh sieve, deionized water was added at a solid-liquid ratio of 1:10 g / mL, and ultrasonic treatment was performed at 40 kHz and 300 W for 20 min. The resulting distillate was steam distilled at 120°C for 2 h to obtain the mint extract. (5) Preparation of tea oil burn dressing Mixing the Wulingshen extract, the Bletilla striata extract, and the peppermint extract in a weight ratio of 5:3:1.5 to obtain a traditional Chinese medicine extract; Weigh 40 parts of tea oil, 10 parts of Chinese herbal extract, 10 parts of chitosan quaternary ammonium salt and 5 parts of calcium alginate according to weight; Chitosan quaternary ammonium salt was stirred with 10 times its weight of deionized water at 38°C and 350 rpm for 28 minutes to obtain a chitosan quaternary ammonium salt solution; tea oil was slowly added to the chitosan quaternary ammonium salt solution, stirred at 900 rpm for 20 minutes, calcium alginate and traditional Chinese medicine extract were added, homogenized at 1100 rpm for 15 minutes, and the pH value was adjusted to 6.5 to obtain a tea oil burn and scald dressing.

[0042] Comparative Example 1 The difference between this comparative example and Example 1 is that the preparation method of tea oil is a cold pressing method: the tea seed powder is dried to a moisture content of 3% (w / w), crushed through a 10-mesh sieve, pre-pressed at 8 MPa and 35°C for 40 minutes, and then main-pressed at 23 MPa and 35°C for 50 minutes to obtain tea oil.

[0043] Comparative Example 2 The difference between this comparative example and Example 1 is that tea oil is replaced by sesame oil, the preparation methods of sesame oil and tea oil are the same, and tea seeds are replaced by sesame.

[0044] Comparative Example 3 The difference between this comparative example and Example 1 is that the Chinese herbal medicine extract consists of a Wulingshen extract and a mint extract in a weight ratio of 6:1.

[0045] Comparative Example 4 The difference between this comparative example and Example 1 is that the Chinese medicinal extract consists of Bletilla striata extract and Mentha arvense extract in a weight ratio of 6:1.

[0046] Comparative Example 5 The difference between this comparative example and Example 1 is that: (2) the composite microorganisms in the preparation of the Wulingshen extract are Bifidobacterium bifidum ATCC29521 (bacterial activity 50,000 CFU / g) and Lactobacillus delbrueckii CICC ® 6289 (active bacteria 120,000 CFU / g) was composed in a mass ratio of 3:2, and the fermentation conditions remained unchanged.

[0047] Comparative Example 6 The difference between this comparative example and Example 1 is that: (3) the preparation method of the Bletilla striata extract is as follows: the Bletilla striata is crushed through a 550-mesh sieve, deionized water is added according to a solid-liquid ratio of 1:10 g / mL, extraction is carried out at 70°C for 30 min, filtration is performed, concentration is carried out, and drying is carried out to a water content of 4% (w / w) to obtain the Bletilla striata extract.

[0048] Test Example 1 Antibacterial testing The antibacterial properties of the burn and scald dressings of Examples 1-3 and Comparative Examples 1-6 were tested respectively.

[0049] The activated Escherichia coli culture (CMCC(B)44102, concentration 2.5×10 6 CFU / mL), Staphylococcus aureus culture (CMCC(B)26003, concentration 1.3×10 6 CFU / mL) and Candida albicans liquid (CMCC(F)98001, concentration 7.5×10 5 1 ml of each of the 100 mL CFU / mL (0.1 mL) was dropwise added to the surface of the burn and scald dressings (1 mm thick) of Examples 1-2 and Comparative Examples 1-6, respectively, and incubated at a constant temperature for 24 h. 10 mL of sterile physiological saline was added for dilution, and the diluted bacterial solution was transferred and spread on broth agar plates. Escherichia coli and Staphylococcus aureus were incubated at 37°C for 48 h, and the broth agar plates for Candida albicans were incubated at 28°C for 48 h. The number of colonies on the culture medium was counted, and a blank experiment was performed at the same time. Each group was tested in parallel three times, and the antibacterial rate of each group was calculated. The specific results are shown in Table 1.

[0050] Antibacterial rate (%) = (number of colonies in the blank experiment - number of colonies in the sample experiment) × 100% / number of colonies in the blank experiment.

[0051] Table 1 Antibacterial properties of burn and scald dressings in each group

[0052] As shown in Table 1, all groups of burn and scald dressings exhibited an inhibitory effect on microorganisms. Examples 1-3 exhibited superior antibacterial effects against Escherichia coli, Staphylococcus aureus, and Candida albicans. A comparison of Example 1 with Comparative Examples 1-6 reveals that the preparation of tea oil, replacing tea oil with sesame oil, omitting Bletilla striata extract, omitting Urinary Glycyrrhiza uralensis extract, altering the Urinary Glycyrrhiza uralensis fermentation microorganism, and changing the Bletilla striata extraction solvent all impacted the antibacterial properties of the burn and scald dressings.

[0053] Test Example 2 Animal experiments 120 SD rats, half male and half female, were anesthetized by intraperitoneal injection of 3% sodium pentobarbital (30 mL / kg). The back was depilated 2 cm from the scapula (3 cm × 3 cm) and disinfected. The model was created using a scald instrument (pressure scale 500g, temperature 105°C, duration 18 s). The burn area was 5 cm 2 When the skin of the rats at the depilated area turned white and hardened and scabby skin was observed in the burned area the next day, it indicated that the deep second-degree burn model was successfully established.

[0054] 110 rats with deep second-degree burn models were successfully established, half male and half female, and divided equally into 11 groups. The burn sites were smeared with a 1.5 mm thick Demolin skin wound dressing (positive group), tea oil burn dressings of Examples 1-3, and tea oil burn dressings of Comparative Examples 1-6, respectively. The model group was not smeared with drugs, and the dressings were changed every 3 days until the wound healed.

[0055] During the experiment, the model rats cleaned their wounds with normal saline daily before dosing. The healing status of the burn wounds in each group (including color and hardness) was observed and photographed. Photoshop software was used to analyze the burn wound healing status, and the burn wound healing rate was calculated. The wound healing rates and complete healing times for each group on days 6, 9, and 15 are shown in Table 2.

[0056] Wound healing rate (%) = (initial burn area - unhealed wound area) / initial burn area × 100%.

[0057] Table 2 Wound healing rate and complete healing time of each group

[0058] As can be seen from the data in Table 2, overall, the positive group, the embodiment group, and the comparative example group promoted the healing of burn wounds to varying degrees, and the healing effects of the positive group and the embodiment group were comparable. Comparison of the data in Example 1 and Comparative Examples 1-2 shows that replacing tea oil with sesame oil slows down the healing of burn wounds. Comparison of the data in Example 1 and Comparative Examples 3-4 shows that the extract of Ulingshen and the extract of Bletilla striata have a synergistic effect in treating burns. Comparison of the data in Example 1 and Comparative Examples 5-6 shows that the tea oil burn dressings obtained by different extraction methods have different effects.

[0059] From the above data, it can be seen that the tea oil burn and scald dressing obtained by the preparation method of the present invention has the effects of antibacterial and promoting the healing of burn wounds.

[0060] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A tea oil burn dressing, characterized in that: The invention comprises the following raw materials in parts by weight: 30-40 parts of tea oil, 5-10 parts of traditional Chinese medicine extract, 5-10 parts of chitosan quaternary ammonium salt and 2-5 parts of calcium alginate; the traditional Chinese medicine extract comprises radix scutellariae extract, radix scutellariae extract and mint extract.

2. The tea oil burn and scald dressing according to claim 1, wherein The tea oil is obtained by extracting tea seeds through supercritical CO2.

3. The tea oil burn and scald dressing according to claim 1, wherein The black ginseng extract is obtained by fermenting black ginseng sclerotia with composite microorganisms.

4. The tea oil burn and scald dressing according to claim 1, characterized in that The white ampelopsis extract is obtained by ultrasonically extracting the white ampelopsis from an ethanol solution and purifying it with a macroporous adsorption resin.

5. The tea oil burn and scald dressing according to claim 1, characterized in that The mint extract is obtained from mint leaves through steam distillation.

6. The tea oil burn and scald dressing according to any one of claims 3 to 5, characterized in that: The weight ratio of the Wulingshen extract, the Bletilla striata extract and the peppermint extract is (3-5):(1-3):(0.5-1.5).

7. The method for preparing the tea oil burn and scald dressing according to any one of claims 1 to 6, characterized in that: include: Chitosan quaternary ammonium salt is dissolved in deionized water to obtain a chitosan quaternary ammonium salt solution; tea oil and the chitosan quaternary ammonium salt solution are mixed, calcium alginate and a traditional Chinese medicine extract are added in sequence, homogenized, pH value adjusted, and sterilized to obtain the burn and scald dressing.

8. The preparation method according to claim 7, characterized in that The homogenization speed is 800-1200 rpm, and the time is 15-20 minutes.

9. The preparation method according to claim 7, characterized in that The pH value is 6-6.

5.

10. Use of the tea oil burn and scald dressing according to claim 1 in preparing burn and scald medicines.

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