Preparation method and application of traditional Chinese medicine combined leavening for protecting liver and dispelling effects of alcohol

Abstract

The invention relates to a traditional Chinese medicine combined fermentation product for protecting liver and alleviating hangover, which can be effectively used for alleviating hangover, quickly relieving adverse reaction after excessive drinking, protecting physical and psychological health, and realizing application in preparation of a hangover alleviating medicine, and is prepared by using a monascus purpureus 7-3 fermented traditional Chinese medicine composition. The preparation method comprises the following steps: grinding the traditional Chinese medicine raw materials into powder, sieving, sterilizing, inoculating monascus purpureus 7-3 spore liquid, culturing in a shaking table, collecting supernate, adding water, extracting, filtering, combining the two filtrates and the supernate, uniformly mixing, concentrating under reduced pressure to form fluid extract, freeze-drying, and packaging. And preparing freeze-dried powder. According to the present invention, the raw materials are rich, the preparation method is simple, the edible fungi and the traditional Chinese medicine composition are effectively combined, the traditional Chinese medicine is prepared by using the main-auxiliary compatibility relationship of monarch, minister, assistant and guide through the specific fermentation method, the effects of sobering, drunkenness symptom relieving, detoxification and liver protection are provided, and the economic and social benefits are significant.

Description

Technical Field

[0001] The present invention relates to the field of traditional Chinese medicine, and in particular to a preparation method and application of a traditional Chinese medicine combination fermentation product for protecting the liver and relieving alcohol. Background Art

[0002] Wine culture has a history of thousands of years and is widespread in the world. Moderate drinking can relax people's minds and benefit their health. However, excessive drinking can cause dizziness, vomiting, cognitive impairment, nausea, reduced mobility, increased blood pressure, increased heart burden and other discomforts, which may continue to affect daily life the next day. In the long run, it will also have adverse effects on health. Traditional Chinese medicine has multi-targeted properties. China has always used traditional Chinese medicine as a hangover remedy. For example, "Compendium of Materia Medica" records that "kudzu flower can relieve alcohol and invigorate the spleen, and relieve alcohol poisoning", and "Supplement to Materia Medica" states that "Hovenia dulcis can relieve alcohol poisoning". Due to the limited efficacy of single ingredients, although there are traditional Chinese medicine combination hangover prescriptions, the hangover effect is limited due to the unscientific and unreasonable combination of ingredients.

[0003] Red yeast rice has been used as both a medicinal and edible food since ancient times. The earliest record of this in the Han Dynasty's "Seven Commentaries" states, "Guazhou red yeast rice is blended and kneaded, resulting in a soft, smooth, and creamy texture that flows easily in the mouth," highlighting its edible value. Its unique color and texture add a unique flavor and hue to traditional cuisine. Its fermentation products are primarily used in food and pharmaceutical applications. Modern research indicates that the metabolites of Monascus purpureus include red pigments, yellow pigments, orange pigments, organic acids, sterols, decahydronaphthalene derivatives, flavonoids, polysaccharides, enzymes (amylase, saccharifying enzyme, protease, etc.), and other substances, exhibiting various activities, including antioxidant, antibacterial, anti-atherosclerotic, anti-obesity, hypolipidemic, hypoglycemic, and anti-tumor activities. my country stipulates that the species of Monascus purpureus used in traditional Chinese medicine and health supplements is Monascus purpureus. The United States Pharmacopoeia (USP) requires that the fermentation strain for red yeast rice be Monascus purpureus NTU 568. The USP even uses Monascin and Ankaflavin as quality control markers for red yeast rice. Monascinascu purpureus7-3 does not produce lovastatin and citrinin, but produces monascin and ankaflavin, so the metabolites of the strain meet the requirements for food dietary supplements.

[0004] So, is it possible to combine red yeast rice with traditional Chinese medicine, ferment it, and make a traditional Chinese medicine combination fermentation product that can protect the liver and relieve alcohol, so as to effectively relieve alcohol, quickly relieve the adverse reactions after excessive drinking, and protect physical and mental health? However, there has been no public report so far. Summary of the Invention

[0005] In view of the above situation, in order to overcome the shortcomings of the existing technology, the purpose of the present invention is to provide a traditional Chinese medicine combination fermentation product for protecting the liver and relieving alcohol, which can be effectively used to relieve alcohol, quickly relieve the adverse reactions after excessive drinking, protect physical and mental health, and realize the application problem in the preparation of alcohol-relieving drugs.

[0006] The technical solution provided by the present invention is: a preparation method of a traditional Chinese medicine combination fermentation product for protecting the liver and relieving alcohol, wherein the traditional Chinese medicine combination fermentation product is prepared by fermenting a traditional Chinese medicine composition with Monascus purpurogenum 7-3, wherein the traditional Chinese medicine is composed of the following traditional Chinese medicine raw materials in parts by weight: 1-3 parts of kudzu flower, 3-8 parts of hovenia dulcis fruit, 1-2 parts of amomum villosum, 1-2 parts of cardamom, 1-8 parts of hawthorn, 0.5-2 parts of ginseng, 0.5-2 parts of poria and 0.5-2 parts of tangerine peel, and the preparation method is as follows: pulverizing the traditional Chinese medicine raw materials, sieving through an 80-100 mesh sieve into fine powder, sterilizing, and inoculating at room temperature at a concentration of 1×10 5 CFU / mL of Monascus purpurogenum 7-3 spore liquid is placed in a shaker, cultured at room temperature and a speed of 100-200 r / min for 7-21 days, and the fermentation supernatant of the traditional Chinese medicine composition is collected. The residue is added with water 5-10 times the weight volume of the traditional Chinese medicine raw material and extracted twice, each time for 0.5-2h, filtered to obtain two filtrates, the two filtrates and the supernatant are combined, mixed, and concentrated under reduced pressure to a fluid extract with a density of 1.0-1.1g / mL, and then freeze-dried to prepare a freeze-dried powder. The weight and volume refer to solids in g and liquids in mL;

[0007] The Monascus purpureus 7-3 is classified and named Monascinascu purpureus, with a preservation number of CGMCC No. 41791; the preservation unit is the General Microbiology Center of the China Culture Collection Administration; the preservation address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences; the preservation time is March 5, 2025.

[0008] Application of the liver-protecting and alcohol-relieving Chinese medicinal combination fermentation product prepared by the above method in the preparation of liver-protecting and alcohol-relieving drugs.

[0009] The invention has abundant raw materials and a simple preparation method, effectively combines edible strains and a traditional Chinese medicine composition, adopts a primary-secondary compatibility relationship of monarch, minister, assistant and envoy in traditional Chinese medicine, and is prepared into a fermented alcohol-relieving traditional Chinese medicine composition through a specific fermentation method. The traditional Chinese medicine composition has the functions of sobering up, relieving drunkenness symptoms, detoxifying and protecting the liver, can be effectively used to relieve adverse reactions after excessive drinking, protect physical and mental health, and has significant economic and social benefits. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 This is a colony morphology diagram of Monascinascu purpureus 7-3 of the present invention; (Abstract Figure)

[0011] Figure 2 This figure shows the effect of the fermentation product of the present invention on the ADH activity in mouse liver;

[0012] Figure 3 This figure shows the effect of the fermentation product of the present invention on the ALDH activity in mouse liver;

[0013] Figure 4 This figure shows the effect of the fermentation product of the present invention on the ALT activity in mouse serum;

[0014] Figure 5 This figure shows the effect of the fermentation product of the present invention on the AST activity in mouse serum;

[0015] Figure 6 This figure shows the effect of the fermentation product of the present invention on TC activity in mouse liver;

[0016] Figure 7 This figure shows the effect of the fermentation product of the present invention on TG activity in mouse liver;

[0017] Figure 8 This is a graph showing the HE results of the mouse livers in the control group and the experimental group of the present invention. DETAILED DESCRIPTION

[0018] The specific implementation methods of the present invention are described in detail below with reference to examples and specific situations.

[0019] Example 1:

[0020] A method for preparing a fermented combination of Chinese medicinal materials for protecting the liver and relieving alcohol consumption, wherein the Chinese medicinal materials are composed of the following Chinese medicinal materials in parts by weight: 1 part of Pueraria lobata flower, 3 parts of Hovenia dulcis fruit, 1 part of Amomum villosum, 1 part of Cardamom, 1 part of Crataegus pinnatifida, 0.5 parts of Ginseng, 0.5 parts of Poria cocos, and 0.5 parts of Tangerine peel. The preparation method is as follows: pulverizing the Chinese medicinal materials, sieving through an 80-100 mesh sieve to form a fine powder, sterilizing, and inoculating at room temperature at a concentration of 1×10 5 CFU / mL of Monascus purpurogenum spore liquid is placed in a shaker and cultured at room temperature at a rotation speed of 100-200 r / min for 7-21 days. The fermentation supernatant of the traditional Chinese medicine composition is collected, and the residue is added with water 5-10 times the weight volume of the traditional Chinese medicine raw material for extraction twice, each time for 0.5-2 hours, filtered to obtain two filtrates, and the two filtrates and the supernatant are combined and mixed. The mixture is concentrated under reduced pressure to a fluid extract with a density of 1.0-1.1 g / mL, and then freeze-dried to prepare a freeze-dried powder with a yield of 16.72%.

[0021] Example 2:

[0022] A method for preparing a fermented Chinese herbal combination for protecting the liver and relieving alcohol consumption. The Chinese herbal combination comprises the following raw materials, calculated by weight: 1 part of kudzu flower, 5 parts of hovenia dulcis fruit, 2 parts of amomum villosum, 3 parts of cardamom, 3 parts of hawthorn, 1.5 parts of ginseng, 1.5 parts of poria, and 1.5 parts of dried tangerine peel. The preparation method is the same as that of Example 1, and the yield is 19.44%.

[0023] Example 3:

[0024] A method for preparing a fermented Chinese herbal combination for protecting the liver and relieving alcohol consumption. The Chinese herbal combination comprises the following raw materials, calculated by weight: 2.5 parts of kudzu flower, 7.5 parts of hovenia dulcis fruit, 2.5 parts of amomum villosum, 2.5 parts of cardamom, 5 parts of hawthorn, 1 part of ginseng, 1 part of poria, and 1 part of dried tangerine peel. The preparation method is the same as that of Example 1, and the yield is 21.95%.

[0025] Example 4:

[0026] A method for preparing a fermented Chinese medicine combination for protecting the liver and relieving alcohol, wherein the Chinese medicine is composed of the following Chinese medicinal raw materials by weight: 3 parts of Pueraria lobata flower, 8 parts of Hovenia dulcis fruit, 2 parts of Amomum villosum, 2 parts of Cardamom, 8 parts of Crataegus pinnatifida, 2 parts of Ginseng, 2 parts of Poria cocos, and 2 parts of Tangerine peel. The preparation method is the same as that of Example 1, and the yield is 29.63%.

[0027] The freeze-dried powder of the fermented product obtained in each of the above examples had a calculated yield of 16.72-29.63%. The fermented Chinese medicine composition exhibited a characteristic aroma of the traditional Chinese medicine pieces combined with a slightly sweet flavor and a brown color. Numerous mycelial pellets were present in the liquid, and red mycelium was stained on the walls of the fermentation tank. The freeze-dried powder was characterized by a brown powder with a slightly sweet and fragrant aroma, characteristic of traditional Chinese medicine. The powder was relatively hygroscopic, had a slightly high viscosity, exhibited a certain degree of fluidity, and was readily soluble in water.

[0028] According to the composition given in the above embodiment, any amount of fermentation product can be prepared as needed. The examples given are only used to illustrate the specific implementation methods of the present invention, and are not used to limit the scope of protection of the present invention. Any technical solution that is essentially the same as the technical solution of the present application and is made by equivalent replacement or transformation means shall fall within the scope of protection of the present invention.

[0029] In the above-mentioned drug combinations, wherein:

[0030] Kudzu flower: sweet in taste, cool in nature, enters the spleen and stomach meridians; can relieve alcohol, invigorate the spleen, and stop bleeding.

[0031] Hovenia dulcis (Huang Tzu) Fruit: neutral in nature, sweet in flavor. Enters the Stomach Meridian; quenches thirst, relieves restlessness, clears dampness and heat, and detoxifies alcohol.

[0032] Amomum villosum: warm in nature, pungent in flavor. It enters the stomach, spleen, and kidney meridians; it eliminates dampness and stimulates appetite, warms the spleen and stops diarrhea, regulates qi and stabilizes pregnancy.

[0033] Cardamom: Warm in nature, pungent in flavor. Enters the spleen and stomach meridians; eliminates dampness, relieves abdominal distension, promotes qi and warms the middle, and stimulates appetite and aids digestion.

[0034] Hawthorn: slightly warm in nature, sour and sweet in flavor. It enters the spleen, stomach, and liver meridians; it helps with digestion, strengthens the spleen, and promotes qi and eliminates blood stasis.

[0035] Ginseng: Warm in nature, sweet with a slightly bitter taste. It enters the Heart, Lung, Spleen, and Kidney meridians; it greatly replenishes vital energy, strengthens the meridians, strengthens the spleen and lungs, and promotes the production of body fluids and calms the mind.

[0036] Poria: neutral in nature, sweet and bland in flavor. It enters the Heart, Lung, Spleen, and Kidney meridians; it promotes diuresis, dissipates dampness, strengthens the spleen, and calms the mind.

[0037] Tangerine peel: Spicy and bitter in taste, warm in nature. Enters the spleen and lung meridians; regulates qi and stimulates appetite, dries dampness and resolves phlegm, and treats spleen and stomach diseases.

[0038] According to the principles of traditional Chinese medicine, the above components are classified into the main and secondary monarch, minister, assistant and envoy, and are functionally related to each other and work together to play a role. In the prescription, kudzu flower and hovenia dulcis are the monarch drugs. Kudzu flower can relieve alcohol and invigorate the spleen, and can specifically reduce the internal dampness and heat caused by excessive drinking, promote the decomposition of alcohol in the body, and relieve the discomfort after drinking, such as dizziness, headache, nausea and vomiting. Hovenia dulcis can clear away heat, promote the production of body fluid, and relieve alcohol toxins, and can enhance the liver's detoxification function, accelerate alcohol metabolism, reduce blood alcohol concentration, and effectively relieve thirst, irritability and other conditions after drinking; Amomum villosum, cardamom and hawthorn are the ministerial drugs. Amomum villosum and the middle , promote qi circulation, cardamom stops vomiting, both of them can help the spleen and stomach transport and transform, improve the spleen and stomach qi stagnation, dampness blocking the middle burner, abdominal distension, loss of appetite, etc. caused by drinking, promote digestion and absorption, strengthen the spleen and stomach function, and enhance the body's ability to metabolize alcohol and drugs. Hawthorn can help digestion and eliminate accumulation, especially for stagnation of food and alcohol, and assist in resolving food accumulation after drinking; ginseng and poria are adjuvants, which can replenish qi and strengthen the spleen, prevent alcohol-detoxifying drugs from damaging the vital energy, strengthen the spleen and eliminate dampness, enhance the effect of removing dampness, and relieve edema and fatigue after drinking; tangerine peel is the guiding drug, which can regulate qi and harmonize the effects of various drugs, and guide the medicinal power to the spleen and stomach.

[0039] The fermented product of the Chinese herbal combination has the function of sobering up, which is reflected in relieving physical discomfort after drinking, accelerating the sobering up to a certain extent, making people recover more quickly, protecting liver health, reducing alcohol damage to the liver, and reducing the risk of liver disease. It has good efficacy and high efficiency, and has achieved very good beneficial technical results through experiments. The relevant experimental materials are as follows: Experiment 1: Animal experiment

[0040] KM mice were randomly divided into groups, wherein: the first group was a blank group, which was gavaged with an equal volume of normal saline; the second group was a model group; the third group was Example 1 group, which was administered at a dose of 100 mg / kg; the fourth group was Example 2 group, which was administered at a dose of 100 mg / kg; the fifth group was Example 3 group, which was administered at a dose of 100 mg / kg; and the sixth group was Example 4 group, which was administered at a dose of 100 mg / kg; after 3 days of preventive administration, the mice in each group were fasted for 12 hours, and then treated according to the above conditions of each group. Except for the first group, the mice in each group were gavaged with 0.15 mL / 10 g body weight of 52-proof liquor, and the drunkenness time of the mice in each group was observed and recorded; the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum were measured according to the instructions of the kit; the activities of alcohol dehydrogenase (ADH), acetaldehyde dehydrogenase (ALDH), total cholesterol (TC), and triglyceride (TG) in liver tissue were measured.

[0041] After each group of mice was gavaged with liquor, timing was immediately started and a righting reflex test was performed to determine whether the mice were drunk. The drunkenness time and soberness time were recorded, as shown in the table below:

[0042] Table 1 Effects of fermentation products on sobering up mice

[0043]

[0044]

[0045] As shown in Table 1, the average duration of intoxication in the drug-treated group increased, indicating that the Example group was able to delay the time from drinking to fainting and then unconsciousness. Compared with the model group, the sobering time in the Example group was also significantly shortened. These results indicate that the Example group has a significant sobering effect and can alleviate the symptoms of intoxication.

[0046] like Figure 2 、 Figure 3 As shown, compared with the normal group, the ADH and ALDH activities in the model group were significantly increased (P < 0.05, P < 0.01); compared with the model group, Example 1, 2, 3, and 4 groups all significantly increased the ADH and ALDH activities in the liver tissue of drunken mice. Therefore, the results show that the fermentation product of the present invention does have the effect of treating hangover; among them, Example 3 group of the present invention has the best effect in promoting ADH and ALDH.

[0047] like Figure 4 、 Figure 5As shown, compared with the normal group, the serum ALT and AST levels of the model group mice were significantly increased; compared with the model group, the ALT and AST levels of Example 1, 2, 3, and 4 groups were significantly decreased (P < 0.05, P < 0.01). The Example groups not only promoted the activity of alcohol dehydrogenase and acetaldehyde dehydrogenase in the livers of drunken mice, reducing the damage caused by ethanol to the body, but also reduced the activity of ALT and AST in the blood, which are indicators of liver function, indicating that traditional Chinese medicine fermentation products can also reduce the damage caused by ethanol to the liver.

[0048] Excessive alcohol consumption causes peroxidation of phospholipids in liver cell membranes, generating a large number of lipid free radicals, leading to β-oxidation disorders of fatty acids, metabolic disorders of TG in liver cells, inducing fatty degeneration, resulting in obstruction of blood lipid transport, and a significant increase in TG and TC levels. Therefore, the level of TC and TG in the liver can reflect the abnormal degree of fat accumulation in liver cells. Figure 6 、 Figure 7 As shown, compared with the normal group, the TC and TG levels in the liver tissue of the model group mice were significantly increased. Compared with the model group, the groups of Examples 1, 2, 3, and 4 were able to reduce the TC and TG levels to varying degrees, with the group of Example 3 having the best effect.

[0049] HE staining of the livers of mice in each group revealed that the tissue cells in the blank group were uniformly stained, neatly arranged, with clear structure and normal morphology. In the model group, the liver cells were significantly damaged, with incomplete morphological cell structure, disordered intercellular arrangement, and blurred intercellular boundaries. Extensive vacuolar degeneration also occurred, and a small number of cells showed signs of death. Compared with the alcohol model group, the example groups were able to reduce vacuolar degeneration in the cytoplasm. The mouse liver lobules were clearly demarcated and arranged regularly, and the liver cells were round and plump; the liver plates were arranged regularly and neatly, and the liver sinusoids showed no obvious expansion or compression; there were no obvious abnormalities in the portal tracts between adjacent liver lobules; and no obvious inflammation was observed. Therefore, it can be seen that the example groups all have a certain protective effect on acute alcoholic liver injury in mice, among which Example 3 group had the best effect.

[0050] Experiment 2: Drinking experiment

[0051] This study selected 40 drinking subjects for four parallel experiments (Examples 1-4), with two treatment conditions set for each group:

[0052] The experimental group (Group A) consisted of 5 people who drank a fixed amount of alcohol and then took the fermented products prepared in Examples 1, 2, 3, and 4.

[0053] The 5 people in the blank control group (Group B) drank the same amount of water and then took the same fermented product, see the table below:

[0054] Table 2 Statistics of indicators of drinking population

[0055]

[0056] In the decanting time index, * indicates that the decanting time is 0-30 minutes; ** indicates that the decanting time is 30-60 minutes, and *** indicates that the decanting time is more than 1 hour.

[0057] Among the symptoms after drinking, * indicates no nausea, vomiting, dizziness, unsteady gait and other discomfort symptoms; ** indicates any one of nausea, vomiting, dizziness, and unsteady gait symptoms; *** indicates at least two of nausea, vomiting, dizziness, and unsteady gait symptoms;

[0058] Regarding stomach sensation, * indicates no symptoms of stomach discomfort or loss of appetite; ** indicates any one of stomach discomfort or loss of appetite; *** indicates both stomach discomfort and loss of appetite.

[0059] The data analysis in Table 2 shows that the fermented product of the Chinese herbal combination exhibited a sustained and stable intervention effect in the alcohol metabolism process, and the vital signs of the subjects were stable when they were not drinking, confirming that it has both alcohol-relieving effects and safety.

[0060] In summary, the drug combination of the traditional Chinese medicine combination fermentation product of the present invention is scientific and reasonable. It adopts a unique preparation method, and the traditional Chinese medicine composition is fermented using the newly screened Monascus purpurogenus 7-3 to obtain the traditional Chinese medicine combination fermentation product, which is effectively used for sobering up and quickly alleviating adverse reactions after excessive drinking, detoxifying and protecting the liver, and protecting physical and mental health. It is a major innovation in sobering up and protecting drugs, and has significant economic and social benefits.

Claims

1. A method for preparing a fermented Chinese herbal combination for liver protection and hangover relief, wherein the fermented Chinese herbal combination is prepared by fermenting a Chinese herbal composition with Monascus purpurogenus 7-3, wherein the Chinese herbal composition is composed of the following Chinese herbal raw materials in parts by weight: 1-3 parts of Pueraria flowers, 3-8 parts of Hovenia dulcis fruits, 1-2 parts of Amomum villosum, 1-2 parts of Cardamom, 1-8 parts of Hawthorn, 0.5-2 parts of Ginseng, 0.5-2 parts of Poria cocos and 0.5-2 parts of Tangerine peel. The preparation method is as follows: grind the Chinese medicinal materials into powder, sieve through 80-100 mesh to form fine powder, sterilize, and inoculate at room temperature at a concentration of 1×10 5 CFU / mL of Monascus purpurogenum 7-3 spore liquid is placed in a shaker, cultured at room temperature and a speed of 100-200 r / min for 7-21 days, and the fermentation supernatant of the traditional Chinese medicine composition is collected. The residue is added with water 5-10 times the weight volume of the traditional Chinese medicine raw material and extracted twice, each time for 0.5-2h, filtered to obtain two filtrates, and the two filtrates and the supernatant are combined, mixed, and concentrated under reduced pressure to a fluid extract with a density of 1.0-1.1g / mL, and then freeze-dried to prepare a lyophilized powder. The weight and volume refer to the solid in g and the liquid in mL; The Monascus purpureus 7-3 is classified and named Monascinascu purpureus, with a preservation number of CGMCC No. 41791; the preservation unit is the General Microbiology Center of the China Culture Collection Administration; the preservation address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences; the preservation time is March 5, 2025.

2. The method for preparing the fermented product of the Chinese medicinal combination for protecting liver and relieving alcohol according to claim 1, characterized in that: The Chinese medicine is composed of the following Chinese medicine raw materials in parts by weight: 1 part of Pueraria lobata flower, 3 parts of Hovenia dulcis fruit, 1 part of Amomum villosum, 1 part of Cardamom, 1 part of Hawthorn, 0.5 parts of Ginseng, 0.5 parts of Poria cocos and 0.5 parts of Tangerine peel. The preparation method is as follows: the Chinese medicine raw materials are powdered, sieved through 80-100 mesh to form fine powder, sterilized, and inoculated at room temperature at a concentration of 1×10 5 CFU / mL of Monascus purpurogenum spore liquid is placed in a shaker and cultured at room temperature and a rotation speed of 100-200 r / min for 7-21 days. The fermentation supernatant of the traditional Chinese medicine composition is collected, and the residue is added with water 5-10 times the weight volume of the traditional Chinese medicine raw material for two extractions, each time for 0.5-2 hours. The mixture is filtered to obtain two filtrates, and the two filtrates and the supernatant are combined and mixed. The mixture is concentrated under reduced pressure to a fluid extract with a density of 1.0-1.1 g / mL, and then freeze-dried to prepare a freeze-dried powder.

3. The method for preparing the fermented Chinese medicinal combination for protecting liver and relieving alcohol according to claim 1, characterized in that: The traditional Chinese medicine is composed of the following traditional Chinese medicine raw materials calculated by weight: 1 part of kudzu flower, 5 parts of hovenia dulcis fruit, 2 parts of amomum villosum, 3 parts of cardamom, 3 parts of hawthorn, 1.5 parts of ginseng, 1.5 parts of poria and 1.5 parts of tangerine peel.

4. The method for preparing the fermented product of the Chinese medicinal combination for protecting liver and relieving alcohol according to claim 1, characterized in that: The traditional Chinese medicine is composed of the following traditional Chinese medicine raw materials calculated by weight: 2.5 parts of kudzu flower, 7.5 parts of hovenia dulcis fruit, 2.5 parts of amomum villosum, 2.5 parts of cardamom, 5 parts of hawthorn, 1 part of ginseng, 1 part of poria and 1 part of tangerine peel.

5. The method for preparing the fermented product of the Chinese medicinal combination for protecting liver and relieving alcohol according to claim 1, characterized in that: The traditional Chinese medicine is composed of the following traditional Chinese medicine raw materials calculated by weight: 3 parts of kudzu flower, 8 parts of hovenia dulcis fruit, 2 parts of amomum villosum, 2 parts of cardamom, 8 parts of hawthorn, 2 parts of ginseng, 2 parts of poria and 2 parts of tangerine peel.

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