Mycobacterium tuberculosis detection device pre-loaded with disinfection liquid and method thereof

Abstract

The invention discloses a device and a method for detecting mycobacterium tuberculosis preloaded with sterilizing liquid, and relates to the technical field of microbiological detection. The device comprises an outer shell, one end of the outer shell is sleeved with a sleeve head assembly, a disinfection cavity is formed in the outer shell, one side in the disinfection cavity is fixedly connected with a reaction bin, the other side is fixedly connected with a crushing part, and the crushing part is used for crushing the reaction bin; a blocking piece is arranged between one end of the reaction bin and the interior of the outer shell, a sealing piece is installed between the blocking piece and the reaction bin, the side face of the sealing piece and the inner side wall of the outer shell are provided with a glue sealing ring cavity in the circumferential direction, the glue sealing ring cavity is used for sealing the disinfection cavity and the reaction bin, and the outer shell and the reaction bin are both transparent. The detection sample containing the pathogenic bacteria is killed in time through the preloaded disinfection liquid, the retreatment process of the waste detection sample containing the pathogenic bacteria is avoided, and the detection safety is effectively improved.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial detection, and in particular to a Mycobacterium tuberculosis detection device pre-filled with disinfectant liquid and a method thereof. Background Art

[0002] Mycobacterium tuberculosis is the pathogen that causes tuberculosis. It belongs to the genus Mycobacterium and is a special rod-shaped bacterium with a lipid-rich cell wall that is acid-fast, so it is also called acid-fast bacillus. Its host is mainly humans. It is usually transmitted through aerosols containing the bacteria and can infect almost all tissues of the human body. Among them, pulmonary tuberculosis is the most common lung infection. In the early diagnosis and treatment of Mycobacterium tuberculosis infection, corresponding detection devices are required for auxiliary detection.

[0003] Patent specification CN103937666B discloses a rapid nucleic acid detection kit and method for Mycobacterium tuberculosis. During nucleic acid detection, the technical solution employed is to place an amplification reaction solution and a nucleic acid dye in a detection tube. After the reaction is complete, the tube is repeatedly shaken up and down, and the amplification reaction solution and the nucleic acid dye are mixed without opening the tube cap. Liquid paraffin is also added to the detection tube as a stabilizing liquid to prevent the formation of aerosols during the amplification reaction, thereby avoiding the escape of pathogenic bacteria during the detection process. The shortcoming of this technical solution is that since the amplification reaction liquid itself is used to amplify the DNA and RNA fragments of bacteria, the nucleic acid dye can only be used for color development reaction. The two as detection units have no disinfecting effect on bacteria, and the stabilizing liquid can only be used to prevent the escape of bacteria during bacterial amplification, and it cannot effectively kill bacteria. That is, this technical solution only prevents the escape of bacteria during the detection process, and cannot disinfect the bacteria in time after the detection is successfully carried out. It only delays the disinfecting process, and the actual subsequent disinfecting of pathogens still needs to face the problem of secondary exposure to pathogens in the reaction liquid, which leads to insufficient safety when the detection device is used. Summary of the Invention

[0004] The purpose of the present invention is to provide a Mycobacterium tuberculosis detection device and method pre-loaded with disinfectant. The technical problems to be solved are as follows: the existing Mycobacterium tuberculosis detection device cannot effectively and timely disinfect the bacteria after the bacteria detection, resulting in insufficient safety for use.

[0005] The purpose of the present invention can be achieved through the following technical solutions: A Mycobacterium tuberculosis detection device pre-filled with disinfectant liquid comprises an outer shell, a sleeve assembly is sleeved on one end of the outer shell, a disinfection chamber is provided inside the outer shell, a reaction chamber is fixedly connected to one side of the disinfection chamber, and a crushing member is fixedly connected to the other side, the crushing member is used to crush the reaction chamber; a barrier is provided between one end of the reaction chamber and the interior of the outer shell, a sealing member is installed between the barrier and the reaction chamber, a glue sealing ring cavity is provided along the circumferential direction between the side surface of the sealing member and the inner wall of the outer shell, the glue sealing ring cavity is used to seal the disinfection chamber and the reaction chamber, and the outer shell and the reaction chamber are both transparent; The crushing member is arranged inside the barrier member, a side of the barrier member away from the crushing member is in contact with the sealing member, and the glue sealing ring cavity is arranged on the peripheral side of the barrier member and the sealing member in contact.

[0006] As a further solution of the present invention: the head assembly includes a shell sleeve that is sleeved with the outer shell, a plurality of connectors are provided on one side of the shell sleeve, a cover body is installed between the sides of each connector away from the shell sleeve, and the cover body is sleeved with the shell sleeve.

[0007] As a further solution of the present invention: a slot is provided at one end of the reaction chamber, a reaction chamber is provided on the side of the slot away from the sleeve assembly, the barrier member includes a liquid-proof tube inserted into the slot, a liquid isolation plate is fixedly connected to one side of the liquid-proof tube, and the liquid-proof tube passes through the liquid isolation plate to open a strip hole.

[0008] As a further solution of the present invention: the sealing member includes an inner sealing plate plugged into the strip hole, one side of the inner sealing plate is fixedly connected to an outer sealing plate, and the glue sealing ring cavity is arranged between the peripheral side of the outer sealing plate and the inner side wall of the outer shell.

[0009] As a further solution of the present invention: a reaction layer is provided inside the reaction chamber, and the reaction layer includes an NC membrane, a colloidal gold layer and a water absorbent pad stacked in sequence in a direction close to the crushing piece, and the top of the reaction layer is connected to the strip hole.

[0010] As a further solution of the present invention: the outer peripheral side surface of the liquid-proof tube abuts against the inner side wall of the slot, the inner side wall of the liquid-proof tube abuts against the inner sealing plate, and the adjacent sides of the liquid isolation plate and the outer sealing plate fit together.

[0011] As a further solution of the present invention: disinfection liquid is provided inside the disinfection chamber, the crushing piece is in a pointed cone shape, the reaction chamber is made of glass, and the outer shell is made of elastic plastic.

[0012] A method for detecting Mycobacterium tuberculosis with a pre-installed disinfectant solution comprises the following steps: S1. Place the outer shell vertically, open the cover, inject disinfection liquid into the disinfection chamber, and place the reaction layers in the reaction chamber in sequence; S2. Insert the barrier into the slot and drip the sample to be tested into the strip hole to the reaction layer; S3. Insert the sealing member into the strip hole, drip the sealing glue along the sealing ring cavity, and close the cover; S4. After placing the outer shell horizontally, observe the reaction results and press the outer shell at the crushing part to crush the reaction chamber.

[0013] Beneficial effects of the present invention: In the present invention, a reaction chamber is arranged inside a transparent outer shell, and a disinfectant is injected into the outer shell in advance before the reaction is carried out. At the same time, a pressable crushing piece is arranged inside the outer shell. After the sample liquid to be tested is injected into the reaction chamber, the interior of the outer shell is sealed as a whole. The reaction container is sealed without affecting the observation of the test results. After the reaction is completed, the outer shell is pressed to squeeze the internal crushing piece, so that the crushing piece crushes the reaction chamber. The pre-installed disinfectant is used to kill the test sample containing pathogens in time, thereby avoiding the reprocessing process of the discarded test sample containing pathogens, thereby preventing the generation of pathogen aerosols during secondary processing, and effectively improving the detection safety. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] The present invention will be further described below with reference to the accompanying drawings.

[0015] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 is an exploded view of the present invention; Figure 3 It is a structural schematic diagram of the reaction chamber of the present invention; Figure 4 Schematic diagram of the structure of the barrier member of the present invention; Figure 5 is a half-section view of the present invention; Figure 6 This invention Figure 5 A magnified detail of point A in the middle.

[0016] In the figure: 1. Outer shell; 2. Head assembly; 21. Shell; 22. Connector; 23. Cover; 3. Disinfection chamber; 4. Reaction chamber; 41. Slot; 42. Reaction chamber; 5. Crushing part; 6. Barrier; 61. Liquid-proof tube; 62. Liquid-isolating plate; 63. Strip hole; 7. Sealing part; 71. Inner sealing plate; 72. Outer sealing plate; 8. Glue sealing ring cavity; 9. NC membrane; 10. Colloidal gold layer; 11. Water-absorbing pad. DETAILED DESCRIPTION

[0017] The following will provide a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.

[0018] like Figures 1 to 6 As shown, a Mycobacterium tuberculosis detection device pre-filled with disinfectant liquid includes an outer shell 1, a sleeve assembly 2 is sleeved on one end of the outer shell 1, a disinfection chamber 3 is arranged inside the outer shell 1, a reaction chamber 4 is fixedly connected to one side of the disinfection chamber 3, and a crushing member 5 is fixedly connected to the other side, and the crushing member 5 is used to crush the reaction chamber 4; a barrier 6 is installed between one end of the reaction chamber 4 and the inside of the outer shell 1, a sealing member 7 is inserted between the barrier 6 and the reaction chamber 4, and a glue sealing ring cavity 8 is arranged along the circumferential direction on the side of the sealing member 7 and the inner wall of the outer shell 1, and the glue sealing ring cavity 8 is used to seal the disinfection chamber 3 and the reaction chamber 4. The outer shell 1 and the reaction chamber 4 are both transparent.

[0019] like Figure 1 and Figure 2 As shown, the sleeve assembly 2 includes a shell 21 that is sleeved with the outer shell 1. Two connecting members 22 are provided on one side of the shell 21. A cover 23 is installed on the side of the two connecting members 22 away from the shell 21. The cover 23 is sleeved with the shell 21. It should be noted that the device needs to be sealed after the reaction liquid is injected. Corresponding glue will be used in the sealing process. After the glue is injected, the cover body 23 is used to prevent the glue from leaking.

[0020] like Figures 2 to 4 As shown, a slot 41 is provided at one end of the reaction chamber 4, and a reaction chamber 42 is provided on the side of the slot 41 away from the sleeve assembly 2. The barrier member 6 includes a liquid-proof tube 61 inserted into the slot 41, and one side of the liquid-proof tube 61 is fixedly connected to a liquid barrier plate 62. The liquid-proof tube 61 passes through the liquid barrier plate 62 to form a strip hole 63. The sealing member 7 includes an inner sealing plate 71 inserted into the strip hole 63, and one side of the inner sealing plate 71 is fixedly connected to an outer sealing plate 72. The glue sealing ring cavity 8 is provided between the side surface of the outer sealing plate 72 and the inner side wall of the outer shell 1; It should be noted that the reaction chamber 42 inside the reaction bin 4 is arranged inside the slot 41 at the port. The barrier 6 inserted into the slot 41 is used to prevent the disinfection liquid in the disinfection chamber 3 from entering the reaction chamber 42 in advance through the slot 41. The strip hole 63 serves as the reaction liquid inlet to inject the reaction liquid into the reaction chamber 42 when it is not blocked by the sealing member 7.

[0021] like Figure 1 and Figure 5As shown, a reaction layer is provided inside the reaction chamber 4, and the reaction layer includes an NC membrane 9 (nitrocellulose membrane), a colloidal gold layer 10 and a water-absorbing pad 11 stacked in sequence in a direction close to the crushing member 5, and the top of the reaction layer is connected to the strip hole 63; It should be noted that the device uses the existing colloidal gold method for detecting Mycobacterium tuberculosis, that is, by injecting the test sample into the colloidal gold layer 10, if the sample contains Mycobacterium tuberculosis, a color reaction will occur at the T line of the NC membrane 9, if there is no Mycobacterium tuberculosis in the sample, no color reaction will occur, and in conjunction with the reference color band at the C line quality control area, the presence of pathogens in the sample can be judged; after the sample is injected into the reaction chamber 42 through the strip hole 63, the sample is evenly dispersed into the reaction chamber 42 through the absorbent pad 11, and then a color reaction is carried out. Since the outer shell 1 and the reaction chamber 4 are both transparent, the color reaction can be directly observed outside the outer shell 1.

[0022] like Figure 5 and Figure 6 As shown, the outer peripheral side of the liquid-proof tube 61 abuts against the inner side wall of the slot 41, the inner side wall of the liquid-proof tube 61 abuts against the inner sealing plate 71, the adjacent sides of the liquid-isolating plate 62 and the outer sealing plate 72 fit together, a sealing glue is provided at the sealing ring cavity 8, and a disinfectant liquid is provided inside the disinfection cavity 3; It should be noted that since a large amount of disinfectant is injected into the disinfection chamber 3, and since Mycobacterium tuberculosis dies within two minutes in an environment of 70% alcohol concentration, the disinfectant is preferably 75% alcohol concentration. During the reaction, it is necessary to prevent the disinfectant from entering the reaction chamber 42 from the slot 41 to kill Mycobacterium tuberculosis. Therefore, the blocking effect on the disinfectant is achieved by inserting the blocking member 6 into the slot 41. After the reaction is completed, after the crushing member 5 crushes the reaction chamber 4, it is necessary to prevent the disinfectant from flowing back from the reaction chamber 42 to the slot 41 for discharge. Therefore, glue is arranged in an annular direction at the glue sealing ring cavity 8 to completely seal the internal reaction environment.

[0023] In addition, the crushing piece 5 is in a pointed cone shape, the reaction chamber 4 is preferably made of glass, and the outer shell 1 is preferably made of elastic plastic; It should be noted that after the color development reaction inside the reaction chamber 4 is completed, it is necessary to press the crushing piece 5 from the outside of the outer shell 1 to squeeze the adjacent side of the reaction chamber 4 to achieve the purpose of crushing the reaction chamber 4. Therefore, the outer shell 1 needs to have a certain elasticity, and the reaction chamber 4 is a thin glass that is easy to break. The pointed cone structure of the crushing piece 5 is also easy to achieve the crushing effect of the reaction chamber 4; further, a guide groove or an ultrasonic oscillation hole is provided at the bottom of the disinfection chamber 3 to ensure that the disinfection liquid can evenly infiltrate the broken fragments of the reaction chamber 4, thereby improving the sterilization efficiency.

[0024] A method for using a Mycobacterium tuberculosis detection device pre-installed with a disinfectant, comprising the following steps: S1, vertically place the outer shell 1 (see Figure 5 ), open the cover 23, inject the disinfection liquid into the disinfection chamber 3, and place the reaction layers in the reaction chamber 42 in sequence; this stage is a preparatory step before the reaction, and the disinfection liquid injection height does not exceed the height of the top of the reaction chamber 42; S2, insert the barrier 6 into the slot 41, and drip the sample to be tested into the strip hole 63 into the reaction layer; this stage is the step of injecting the reaction solution; S3, insert the sealing member 7 into the strip hole 63, drip the sealing glue into the sealing ring cavity 8, and close the cover 23; this stage is the step of sealing the reaction chamber 4 and the disinfection chamber 3; S4, after the outer shell 1 is placed horizontally, the reaction result is observed, and the outer shell 1 is pressed at the crushing part 5 to crush the reaction chamber 4; this stage is a step for timely and thorough killing of pathogenic bacteria after observing the reaction result.

[0025] Working principle of the present invention: Place the outer shell 1 vertically, open the cover 23, inject 75% concentration of alcohol into the outer shell 1 as a disinfectant, then put the NC membrane 9, colloidal gold layer 10 and absorbent pad 11 into the reaction chamber 42 in sequence, then insert the seal 7 into the slot 41, drip the sample liquid to be tested into the strip hole 63, after the sample liquid to be tested enters the reaction chamber 42 through the strip hole 63, insert the seal 7 into the strip hole 63, and form a glue sealing ring cavity 8 between the seal 7 and the inner wall of the outer shell 1. Inject glue along the circumferential direction of the glue sealing ring cavity 8 for sealing. At this time, the reaction chamber 4 and the disinfection cavity inside the outer shell 1 are sealed. 3 are completely sealed, and then the cover 23 is closed to prevent part of the glue from overflowing. At the same time, the outer shell 1 is placed horizontally to wait for the color reaction. The test results are recorded after about 15 minutes of color development. Then the outer shell 1 on the side of the crushing piece 5 is pressed. The pointed end of the crushing piece 5 squeezes the glass-like reaction chamber 4, and the alcohol in the disinfection chamber 3 enters the reaction chamber 42 to inactivate the pathogens, thereby achieving safe killing of the pathogens. That is, the pathogens are also killed synchronously when the test is completed, and there is no need to perform subsequent processing on the reaction part, avoiding the generation of pathogen aerosols during the secondary processing process, thereby effectively improving the detection safety.

[0026] The above is a detailed description of an embodiment of the present invention. However, the content described is only a preferred embodiment of the present invention and should not be considered to limit the scope of the present invention. All equivalent changes and improvements made within the scope of the present invention should still fall within the scope of the patent coverage of the present invention.

Claims

1. A Mycobacterium tuberculosis detection device pre-filled with disinfectant, characterized in that: It comprises an outer shell (1), one end of the outer shell (1) is sleeved with a sleeve assembly (2), a sterilization chamber (3) is provided inside the outer shell (1), one side of the sterilization chamber (3) is fixedly connected to a reaction chamber (4), and the other side is fixedly connected to a crushing member (5), and the crushing member (5) is used to crush the reaction chamber (4); A barrier (6) is provided between one end of the reaction chamber (4) and the interior of the outer shell (1), a sealing member (7) is installed between the barrier (6) and the reaction chamber (4), a glue sealing ring cavity (8) is provided along the circumferential direction between the side of the sealing member (7) and the inner side wall of the outer shell (1), and the glue sealing ring cavity (8) is used to seal the disinfection chamber (3) and the reaction chamber (4), and the outer shell (1) and the reaction chamber (4) are both transparent; The crushing member (5) is arranged inside the barrier member (6), the side of the barrier member (6) away from the crushing member (5) is in contact with the sealing member (7), and the glue sealing ring cavity (8) is arranged on the peripheral side of the side where the barrier member (6) and the sealing member (7) are in contact.

2. The Mycobacterium tuberculosis detection device pre-filled with disinfectant according to claim 1, characterized in that: The sleeve assembly (2) comprises a shell (21) sleeved with the outer shell (1); a plurality of connecting members (22) are provided on one side of the shell (21); a cover (23) is installed between the sides of the connecting members (22) away from the shell (21); and the cover (23) is sleeved with the shell (21).

3. The Mycobacterium tuberculosis detection device pre-filled with disinfectant according to claim 1, characterized in that: A slot (41) is provided at one end of the reaction chamber (4), and a reaction chamber (42) is provided on a side of the slot (41) away from the sleeve assembly (2). The barrier member (6) includes a liquid-proof tube (61) inserted into the slot (41), and a liquid isolation plate (62) is fixedly connected to one side of the liquid-proof tube (61). The liquid-proof tube (61) passes through the liquid isolation plate (62) and is provided with a strip hole (63).

4. The Mycobacterium tuberculosis detection device pre-filled with disinfectant according to claim 3, characterized in that: The sealing member (7) includes an inner sealing plate (71) plugged into the strip hole (63), one side of the inner sealing plate (71) is fixedly connected to an outer sealing plate (72), and the glue sealing ring cavity (8) is arranged between the peripheral side surface of the outer sealing plate (72) and the inner side wall of the outer shell (1).

5. The Mycobacterium tuberculosis detection device pre-filled with disinfectant according to claim 3, characterized in that: A reaction layer is provided inside the reaction chamber (4), comprising an NC membrane (9), a colloidal gold layer (10), and a water-absorbing pad (11) stacked in sequence in a direction close to the crushing piece (5), and the top of the reaction layer is connected to the strip hole (63).

6. The Mycobacterium tuberculosis detection device pre-filled with disinfectant according to claim 4, characterized in that: The outer peripheral side surface of the liquid-proof tube (61) abuts against the inner side wall of the slot (41), the inner side wall of the liquid-proof tube (61) abuts against the inner sealing plate (71), and the adjacent sides of the liquid isolation plate (62) and the outer sealing plate (72) fit together.

7. The Mycobacterium tuberculosis detection device pre-filled with disinfectant according to claim 1, characterized in that: The disinfection chamber (3) is provided with disinfection liquid, the crushing piece (5) is in a pointed cone shape, the reaction chamber (4) is made of glass, and the outer shell (1) is made of elastic plastic.

8. The method for detecting Mycobacterium tuberculosis using a pre-filled disinfectant according to claims 1-7, characterized in that: The following steps are involved: S1. Place the outer shell (1) vertically, open the cover (23), inject disinfection liquid into the disinfection chamber (3), and place the reaction layers in the reaction chamber (42) in sequence; S2, inserting the barrier (6) into the slot (41), and dripping the sample to be tested into the strip hole (63) to the reaction layer; S3, insert the sealing member (7) into the strip hole (63), drip the sealing glue in a circumferential direction along the sealing ring cavity (8), and close the cover (23); S4, after placing the outer shell (1) horizontally, observe the reaction result, and press the outer shell (1) at the crushing part (5) to crush the reaction chamber (4).

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