Method for rapidly propagating and culturing cordyceps sinensis based on cordyceps sinensis infection body

Abstract

The invention belongs to the technical field of artificial cultivation of cordyceps sinensis, and particularly relates to a method for rapidly propagating and culturing cordyceps sinensis based on cordyceps sinensis infection bodies, which comprises the following steps: (1) inoculating cordyceps sinensis into a cordyceps sinensis culture solution for culturing to obtain a cordyceps sinensis infection body culture solution; the cordyceps sinensis is a cordyceps sinensis thallus separated from infected hepialus armoricanus larva or a cordyceps sinensis solid culture which has produced conidia; and (2) mixing the winter worm summer fungus infection body culture solution with the hepialus armoricanus larva feed, feeding the hepialus armoricanus larvae, and carrying out grass emergence culture after the hepialus armoricanus larvae are fed to be rigid. According to the method, the rapid propagation culture infection body is high in concentration (can reach 3.3 * 10 < 7 > / mL), short in period (about 40 days) and high in infection rate (not lower than 80%), a large number of cordyceps sinensis infection bodies can be obtained in a short time, the cordyceps sinensis infection bodies are used for infecting cordyceps sinensis hosts to obtain cordyceps sinensis, and the problems that the source of artificially matched and planted cordyceps sinensis strains is unstable, and strain preparations are difficult to store for a long time can be solved.

Description

Technical Field

[0001] The invention belongs to the technical field of artificial cultivation of cordyceps sinensis, and relates to a method for rapidly multiplying and culturing cordyceps sinensis based on cordyceps sinensis infection bodies. Background Art

[0002] Cordyceps sinensis has a wide range of pharmacological effects, including immunomodulatory, anti-tumor, antioxidant, and anti-aging. When the bat moth larvae are infected by the Cordyceps sinensis fungus, they absorb nutrients from the insects and reproduce, causing the insects to be covered with hyphae, which then form a stroma. Under suitable conditions, the stroma produces rod-shaped fruiting bodies, which, together with the insect body, form the Cordyceps sinensis.

[0003] Artificial cultivation of Cordyceps sinensis by simulating the environment and conditions in which Cordyceps sinensis grows is an effective way to make up for the lack of wild resources. Artificial cultivation usually requires the isolation and cultivation of infective Cordyceps sinensis strains. After the strains are effectively infected with the bat moth larvae, Cordyceps sinensis can be obtained by simulating the environment. It can be seen that cultivating infective strains and completing the effective infection of the bat moth larvae are the keys to obtaining Cordyceps sinensis. Specifically, the existing technology usually obtains ascospores or conidia from Cordyceps sinensis and then inoculates them. The inoculation method is, for example, injection inoculation. In this way, the time period for obtaining strains in the existing technology is long, the equipment is complex, and the injection inoculation is traumatic to the larvae, resulting in increased larval mortality. The invention patent application, published as CN1560228A, discloses an infection method that involves first separating the sclerotium (insect body), stroma, and ascocysts of Cordyceps sinensis, culturing them to obtain Hirsutella sinensis. The resulting mycelium and conidia are then made into a suspension and placed directly in the feed for larvae, or the larvae's skin is stained with the suspension. The mycelium and conidia then infect the larvae and, through the circulation of the larval fluids, spread throughout the larvae. The larvae are then consumed, leaving behind sclerotia encased in the larval skin. Finally, stroma emerge from the larval head capsule, completing the sexual form of Cordyceps sinensis. This prior art method requires strain isolation, conidia culture, and then infection inoculation, which is time-consuming.

[0004] In general, developing rapid propagation of Cordyceps sinensis infectious bodies and using simple methods for effective infection are effective ways to increase the yield of Cordyceps sinensis, which is of great significance for large-scale production of Cordyceps sinensis. Summary of the Invention

[0005] The present invention aims to solve the technical problems of long conidia production cycle, low yield and difficulty in infection of Cordyceps sinensis, and provides a method for rapidly expanding and culturing Cordyceps sinensis conidia.

[0006] To achieve the above object, the present invention adopts the following technical solutions: The present invention provides a Cordyceps sinensis infection body culture fluid. The components and dosages in 1L of Cordyceps sinensis culture fluid are as follows: 20g glucose, 5-20g peptone, 5-20g yeast powder, 15-30g bran, 5-20g silkworm pupa powder, 1-3g ammonium citrate, 2-4g potassium dihydrogen phosphate, 1-3g magnesium sulfate, and 3-5g potassium fulvate. Water is added to make the volume 1L. Furthermore, the 1L Cordyceps sinensis culture fluid also contains 5-20g cicada slough powder. The bran, silkworm pupa powder, and cicada slough powder need to be treated with boiling water for 30 minutes and then filtered before use. After adding cicada slough powder to the culture fluid, the infection rate of bat moth larvae is significantly improved.

[0007] The present invention provides a method for rapidly propagating and culturing Cordyceps sinensis based on Cordyceps sinensis infective bodies, comprising: (1) Rapid propagation of Cordyceps sinensis infection bodies: inoculating Cordyceps sinensis into the above-mentioned Cordyceps sinensis culture solution and culturing it to obtain a Cordyceps sinensis infection body culture solution; the Cordyceps sinensis is Cordyceps sinensis fungus isolated from the infected bat moth larvae or a solid culture of Cordyceps sinensis that has produced conidia; The method for removing Cordyceps sinensis fungi from infected bat moth larvae is as follows: Take a healthy, infected bat moth larva, disinfect its surface, and air-dry it. Then, puncture the interpedal membrane of the bat moth's abdomen and remove the Cordyceps sinensis fungus. Disinfect with 75% alcohol using an 18-gauge acupuncture needle. The bat moth larva with the Cordyceps sinensis fungus removed can be used to cultivate Cordyceps sinensis.

[0008] The cordyceps sinensis fungus is cultured in a cordyceps sinensis culture solution, comprising: suspending the cordyceps sinensis fungus in sterile water, adjusting the concentration of the cordyceps sinensis fungus suspension to 10 4 / mL, and then inoculate the Cordyceps sinensis fungus suspension into the Cordyceps sinensis culture solution at an inoculum volume ratio of 5-20%, and culture it statically at 12-20℃ in the dark for 40-50 days.

[0009] (2) Cultivation of Cordyceps sinensis: The culture solution of Cordyceps sinensis infection body is mixed with bat moth larvae feed and a feeding matrix, and the bat moth larvae are fed until the bat moth larvae are rigidified before cultivation of the grass. The feeding matrix is coconut husk or plateau soil.

[0010] Among them, the Cordyceps sinensis infection body and the bat moth larvae feed are based on 100mL Cordyceps sinensis infection body (concentration 3.3*10 7 Mix the mixture at a ratio of 1 kg of bat moth larvae feed to 500 g of feeding substrate.

[0011] The environmental conditions for feeding bat moth larvae are: a temperature of 10-25°C and a relative humidity of 60-70%. The larvae are fed for at least 45 days to allow the Cordyceps sinensis to infect the bat moth larvae. The bat moth larvae are third-instar larvae. The feed composition is a mixed feed consisting of 60-80% w / w Polygonum villosa, 10-20% w / w Potentilla anserina (commonly known as ginseng fruit), 5-10% w / w carrots, and 5-10% w / w golden buckwheat.

[0012] The method involves inoculating bat moth larvae (cordyceps sinensis larvae) fed to rigor mortis into a sterilized Cordyceps sinensis larvae ripping out of the larvae. The ripping out of the larvae is maintained at a humidity of 18-20%, a temperature of 0-12°C, and an air humidity of 80-90%. The larvae are incubated in the dark until the fruiting bodies of the Cordyceps sinensis reach 2.0±0.5 cm. The larvae are then irradiated under full-spectrum light for 2-6 days. The larvae are then harvested when the fruiting bodies essentially cease growth, after being irradiated with full-spectrum light for 6-12 hours daily at 8-15°C. The Cordyceps sinensis ripping out of the larvae ripping out of the larvae is composed, by weight, of 60 parts peat, 10-20 parts plateau soil (or coconut coir), and 10-20 parts vermiculite.

[0013] Compared with the prior art, the present invention has the following beneficial effects: The method of the present invention does not require the isolation and cultivation of ascospores or conidia of Cordyceps sinensis. The infection of the bat moth larvae can be achieved by directly obtaining the Cordyceps sinensis fungus bodies in the bat moth larvae susceptible to the fungus to prepare the infectious bodies.

[0014] A large number of infectious bodies can also be obtained by inoculating the solid culture of Cordyceps sinensis that has produced conidia into the Cordyceps sinensis culture solution for fermentation culture of mycelium, which shows that the Cordyceps sinensis culture solution of the present invention can improve the infectivity of Cordyceps sinensis.

[0015] The process of propagating the infectious body of the present invention is simple, the equipment is simple, and the cycle is short. The infectious body can infect larvae in large quantities by simply feeding them, effectively reducing the preparation cycle of the strain (in the present invention, it takes about 40 days to obtain the Cordyceps sinensis infectious body by culturing the Cordyceps sinensis fungus body, and it takes 40-50 days for the rigid bat moth larvae to emerge from the grass) and the difficulty of use, greatly improving the larvae's infection rate and infection efficiency, and the infectious body is convenient for preparing strain preparations for storage.

[0016] The traditional infection route for bat moth larvae typically involves: a dead insect, a Cordyceps sinensis, collection of ascospores (or isolation and culture of conidia), microcirculation for sporulation, and infection. This process can lead to strain degradation. This method eliminates the tedious process of cultivating dead insects into Cordyceps sinensis and then isolating them. Instead, the present invention achieves sustainable utilization of superior Cordyceps sinensis strains after initial extraction from infected bat moth larvae. Furthermore, using bat moth larvae as a carrier for Cordyceps sinensis growth effectively mitigates the strain degradation problem associated with continuous culture of Hirsutella sinensis on culture media, a common problem in traditional methods.

[0017] With traditional methods, the conidia produced by a single Cordyceps sinensis ascospore can infect approximately 300 bat moth larvae. This method utilizes a natural parasite culture medium for continuous propagation, reducing the likelihood of strain degradation and achieving 5-10 times the efficiency of traditional methods. Furthermore, the bat moth larvae from which the parasites have been removed can be used to cultivate Cordyceps sinensis, facilitating the selection of superior strains. Furthermore, the quality of the resulting Cordyceps sinensis can be correlated with the parasites themselves, enabling reverse selection of desired Cordyceps sinensis strains. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a microscopic image (40×) of the culture fluid of Cordyceps sinensis infection cultured in Example 1 of the present invention. The arrow points to the Cordyceps sinensis.

[0019] Figure 2 This is a microscopic image (40×) of the culture fluid of the Cordyceps sinensis infection cultured in Example 2 of the present invention. The arrow points to the Cordyceps sinensis.

[0020] Figure 3 This is a microscopic image (40×) of the culture fluid of Cordyceps sinensis infection cultured in Example 3 of the present invention. The arrow points to the Cordyceps sinensis.

[0021] Figure 4 This is a microscopic image (40×) of the culture fluid of Cordyceps sinensis infection cultured in Control Group 1 of the present invention. The arrow points to the Cordyceps sinensis.

[0022] Figure 5 This is a microscopic image (40×) of the culture fluid of Cordyceps sinensis infection cultured in Control Group 2 of the present invention. The arrow points to the Cordyceps sinensis.

[0023] Figure 6 This is a microscopic image (10×) of the fungus inside the infected larvae cultured in Example 1 of the present invention. The arrow points to the Cordyceps sinensis fungus.

[0024] Figure 7 This is a microscopic image (10×) of the fungus in the infected larvae cultured in control group 1 of the present invention. The arrow points to the Cordyceps sinensis fungus.

[0025] Figure 8 This is the Cordyceps sinensis cultured in Example 1 of the present invention.

[0026] Figure 9 This is the Cordyceps sinensis cultured in control group 1 of the present invention. DETAILED DESCRIPTION

[0027] To make the objectives, technical solutions, and advantages of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly described below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0028] The Cordyceps sinensis used in the embodiment of the present invention ( Ophiocordycepssinensis The strain number for the strain (Berk.) Sacc. was CQZY-SD01, provided by Chongqing Institute of Traditional Chinese Medicine. Live larvae of the bat moth infected with the fungus were also provided by Chongqing Institute of Traditional Chinese Medicine. The bat moth larvae feed used in the examples consisted of a mixed feed consisting of 70% w / w Polygonum villosa, 20% w / w Potentilla anserina (commonly known as ginseng fruit), 5% w / w carrot, and 5% w / w golden buckwheat. Wheat bran, silkworm pupa powder, cicada slough powder, and potassium fulvate were commercially available.

[0029] Example 1 (1) Obtaining Cordyceps sinensis from infected bat moth larvae: Take a healthy, fungus-infected bat moth larva, disinfect the insect surface with 75% alcohol, dry it, and then use a No. 18 acupuncture needle to puncture the interpedal membrane of the bat moth larva's abdomen. Then take 3-5 drops of Cordyceps sinensis fungi and suspend them in sterile water. Adjust the concentration of the Cordyceps sinensis fungi suspension to 10 4 / mL, and the Cordyceps sinensis fungus suspension was obtained.

[0030] (2) Culture of Cordyceps sinensis infection body: The Cordyceps sinensis fungus suspension obtained in step (1) was inoculated into the Cordyceps sinensis culture solution sterilized by high temperature at an inoculum volume ratio of 10%, and cultured at 12°C in the dark for 40 days to obtain the Cordyceps sinensis infection culture solution ( Figure 1 ), where the concentration of Cordyceps sinensis is 3.3*10 7 pieces / mL.

[0031] The components and amounts for 1 L of Cordyceps sinensis culture are as follows: 20 g glucose, 5 g peptone, 5 g yeast powder, 15 g bran, 5 g silkworm pupa powder, 1 g ammonium citrate, 2 g potassium dihydrogen phosphate, 1 g magnesium sulfate, and 3.17 g potassium fulvate. Add water to 1 L. Boil the bran and silkworm pupa powder in water for 30 minutes and filter before use.

[0032] (3) According to 100mL of Cordyceps sinensis infection culture solution (bacteria concentration 3.3*10 7 The culture medium was mixed with the feed to make the Cordyceps adhere to the surface of the feed, and then mixed with 500g of the feeding matrix. Then, the 3rd instar bat moth larvae were fed for more than 45 days at a temperature of 10-15°C and a relative humidity of 60-70% to allow the Cordyceps to infect the bat moth larvae. The infection rate of the larvae was calculated. After sampling with reference to the Cordyceps sinensis fungus sampling method, the fungus was observed under a microscope. The microscopic image of the fungus in the bat moth larvae infected with the fungus is shown below. Figure 6 shown.

[0033] The bat larvae feed mixed with Cordyceps sinensis is used to feed bat moth larvae. The breeding matrix provides a living space for the bat moths and also provides a matrix basis for the infection of the bat moth body wall. The breeding matrix is coconut husk or plateau soil.

[0034] (4) Raise the larvae until they are rigid, and then plant the rigid Cordyceps sinensis in a sterilized Cordyceps sinensis culture medium. Maintain the medium humidity at 15-20%, the temperature at 0-12°C, and the air humidity at 80-90%. Cultivate in the dark until the Cordyceps sinensis fruiting body grows to 2.0±0.5 cm.

[0035] In this embodiment, the culture medium for Cordyceps sinensis is composed of 60 parts by weight of peat, 15 parts of coconut husks and 15 parts of vermiculite.

[0036] (5) Expose the Cordyceps sinensis to full-spectrum light for 2 days, then culture at 12°C for 8 hours per day until the fruiting bodies of the Cordyceps sinensis basically stop growing. Figure 8 shown.

[0037] Example 2 The same as Example 1, except that 2% cicada shell powder was added to the Cordyceps sinensis culture solution by weight, and the rest were the same. Figure 2 The cicada slough powder is treated with boiling water for 30 minutes and then filtered before use.

[0038] Example 3 The method is basically the same as Example 1, except that: in step (2), the Cordyceps sinensis is derived from a solid culture of Cordyceps sinensis slant culture that has produced conidia. Step (2) is specifically as follows: under sterile conditions, the Cordyceps sinensis slant culture is inoculated into the Cordyceps sinensis culture fluid of Example 1, and the culture is kept in a dark place at 12°C for 50 days to obtain a Cordyceps sinensis infective body culture fluid. The remaining operations are the same as in Example 1. A microscopic image of the Cordyceps sinensis infective body culture fluid in this example is shown in FIG. Figure 3 shown.

[0039] Example 4 The method is basically the same as Example 1, except that: Step (3) is to obtain Cordyceps sinensis to infect the bat moth larvae by injecting the larvae, specifically as follows: under sterile conditions, a microsyringe (needle diameter 0.06 mm) is used to inject 10 μL of Cordyceps sinensis infection suspension (concentration 3.3*10 7 After injection, vaseline was applied to the larval wounds. After one day of low-temperature incubation at 6°C, the larvae were transferred to an environment with a temperature of 10-15°C and a relative humidity of 60-70%, and fed to bat moth larvae for more than 45 days.

[0040] Control group 1 The difference between Control Group 1 and Example 1 is that the Cordyceps sinensis infection culture fluid in Control Group 1 was obtained by adding conidia produced by ascospore microcirculation of Cordyceps sinensis to water. Other operations were the same as in Example 1. The method for producing conidia by ascospore microcirculation of Cordyceps sinensis is described in Chinese invention patent application CN105483073A. A microscopic image of the Cordyceps sinensis infection culture fluid in this example is shown in FIG. Figure 4 After sampling with reference to the Cordyceps sinensis fungus sampling method, the fungus in the bat moth larvae infected with the fungus was observed under a microscope. Figure 7 As shown. Figure 9 shown.

[0041] Control group 2 The difference between Control Group 2 and Example 1 is that the Cordyceps sinensis infection culture fluid of Control Group 2 is obtained by adding conidia obtained by solid fermentation culture of Cordyceps sinensis hyphae to water, and other operations are the same as those in Example 1. The method of solid fermentation culture of conidia of Cordyceps sinensis hyphae is referred to in the literature (Study on the microstructure of conidia formation process of Chinese Hirsutum, Chinese Journal of Microecology 2013.25(7):775-777+781). The Cordyceps sinensis infection fluid of this example is as follows: Figure 5 shown.

[0042] The fungus infection rate, larval survival rate, grass emergence rate and culture period of Examples 1 to 4 of the present invention and Control Groups 1 to 2 are shown in Table 1.

[0043] Table 1. Test results of Cordyceps obtained from Examples 1 to 4 of the present invention and Control Groups 1 to 2 —— Example 1 Feeding Example 2 Feeding Example 3 Feeding Example 4 Injection Control group 1 was fed Control group 2 was fed Infection rate 80% 85% 80% 80% 80% 65% Bat moth larvae survival rate 85% 85% 85% 70% 80% 85% Training cycle 40-50d 40-50d 50-60d 40-50d 90-100d 90-120d Note: Infection rate = number of infected larvae / total number of larvae; survival rate = number of surviving larvae / total number of larvae; culture period is calculated from the time before the infected larvae become rigid to the number of days (d) until the infectious bodies are obtained.

[0044] As can be seen from Table 1, the survival rate of Cordyceps sinensis larvae using the method of Example 1 was 15% higher than that of the traditional injection method (Example 4), and the susceptibility rate was 15% higher than that of infection using conidia produced by traditional solid fermentation of mycelium (Control Group 2), and was comparable to that of infection using conidia produced by ascospore microcirculation (Control Group 1).

[0045] Since the body fluid suspension of a fungus-susceptible bat moth larva (i.e., the Cordyceps sinensis fungus and insect body suspension) can be used to inoculate 5 to 10 bottles of Cordyceps sinensis culture fluid (100 mL per bottle), one bottle of Cordyceps sinensis culture fluid can be used to inoculate 300 to 500 bat moth larvae, and the conidia produced by the ascospores of one Cordyceps sinensis strain can be used to inoculate approximately 300 to 500 bat moth larvae, the inoculation efficiency of the method of the present invention is 5 to 10 times that of the traditional method, and the inoculation efficiency is significantly improved.

[0046] In addition, it can be concluded from the results in Table 1 above that after adding cicada slough powder in Example 2, the infection rate is significantly improved, indicating that cicada slough powder can promote the infection of the infective body; the infection rate of conidia obtained by liquid fermentation of mycelium and the survival rate of bat moth larvae in Example 3 are comparable to those in Example 1, indicating that the Cordyceps sinensis culture solution of the present invention is also suitable for rapid liquid fermentation of mycelium to produce spores.

[0047] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A culture fluid of Cordyceps sinensis infection body, characterized in that: The components and amounts in 1L of Cordyceps sinensis culture medium are as follows: 20g of glucose, 5-20g of peptone, 5-20g of yeast powder, 15-30g of bran, 5-20g of silkworm pupa powder, 1-3g of ammonium citrate, 2-4g of potassium dihydrogen phosphate, 1-3g of magnesium sulfate, and 3-5g of potassium fulvate. Add water to make up to 1L.

2. The Cordyceps sinensis infection body culture fluid according to claim 1, characterized in that: The 1L cordyceps culture solution also contains 5-20g of cicada slough powder. The bran, silkworm pupa powder and cicada slough powder are treated with boiling water and then filtered before use.

3. A method for rapidly propagating and cultivating Cordyceps sinensis based on Cordyceps sinensis infective bodies, characterized in that: include: (1) Inoculating Cordyceps sinensis into the Cordyceps sinensis culture solution of claim 1 or 2 and culturing the solution to obtain a Cordyceps sinensis infection culture solution; the Cordyceps sinensis is Cordyceps sinensis isolated from susceptible bat moth larvae or a solid culture of Cordyceps sinensis that has produced conidia; (2) Mix the culture solution of Cordyceps sinensis infection body with the bat moth larvae feed and feeding matrix, and feed the bat moth larvae. After the bat moth larvae are fed until they are rigid, they are cultured on grass.

4. The method according to claim 3, characterized in that In step (1), the method for removing Cordyceps sinensis fungi from infected bat moth larvae is as follows: Take healthy fungus-infected bat moth larvae, disinfect the insect body surface and dry it, then puncture the interpedicular membrane of the bat moth larvae's abdomen with a needle and take the Cordyceps sinensis fungus body.

5. The method according to claim 3, characterized in that In step (1), the Cordyceps sinensis fungus is cultured in a Cordyceps sinensis culture solution, including: suspending the Cordyceps sinensis fungus in sterile water, adjusting the concentration of the Cordyceps sinensis fungus suspension to 10 4 / mL, and then inoculate the Cordyceps sinensis fungus suspension into the Cordyceps sinensis culture solution at an inoculum volume ratio of 5-20%, and culture it statically at 12-20℃ in the dark for 40-50 days.

6. The method according to claim 3, characterized in that In step (2), the Cordyceps sinensis infection body and the bat moth larvae feed are mixed in the ratio of 100 mL of Cordyceps sinensis infection body culture liquid: 1 kg of bat moth larvae feed: 500 g of feeding matrix. The concentration of the Cordyceps sinensis infection body culture liquid is 3.3*10 7 pieces / mL.

7. The method according to claim 3, characterized in that In step (2), the bat moth larvae are fed for more than 45 days, and the bat moth larvae fed are 3rd instar bat moth larvae.

8. The method according to claim 3, characterized in that In step (2), the feed composition of the bat moth larvae is a mixed feed prepared by 60-80% w / w Polygonum villosa, 10-20% w / w Potentilla anserina, 5-10% w / w carrot, and 5-10% w / w golden buckwheat.

9. The method according to claim 3, characterized in that In step (2), the grass-growing culture is to colonize the bat moth larvae that have been fed to rigidity in the sterilized Cordyceps sinensis rigid insect grass-growing culture matrix, the humidity of the grass-growing matrix is maintained at 18-20%, the temperature is 0-12°C, and the air humidity is 80-90%. The Cordyceps sinensis is cultured in the dark until the fruiting body grows to 2.0±0.5 cm, and then irradiated under full-spectrum light for 2-6 days, and then cultured under full-spectrum light for 6-12 hours per day at 8-15°C until the Cordyceps sinensis fruiting body basically stops growing and is harvested.

10. The method according to claim 9, characterized in that The breeding matrix is coconut husk or plateau soil; the composition of the culture matrix for Cordyceps sinensis dead insects is: by weight, 60 parts of peat, 10-20 parts of plateau soil or coconut husk and 10-20 parts of vermiculite.

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