Skin care composition containing grape seed extract as well as preparation method and application of skin care composition

Abstract

The invention discloses a skin care composition containing a grape seed extract as well as a preparation method and application of the skin care composition. The skin care composition comprises the following components in percentage by mass: 5-10% of a grape seed component embedding body; 5%-20% of an oil phase; 1%-10% of an emulsifier; 70%-85% of a water phase; wherein the grape seed component embedding body is obtained by embedding a grape seed extract with beta-cyclodextrin to form an embedding body and then adding trehalose and mannitol; the skin care composition is obtained by embedding the grape seed component embedding body on the basis of a nano emulsification embedding technology. According to the invention, the stability and transdermal absorption efficiency of the active ingredients are remarkably improved through the synergistic interaction of a nano emulsification embedding technology and a dual embedding system.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care products, and in particular to a skin care composition containing grape seed extract, and a preparation method and application thereof. Background Art

[0002] Grape seed extract, a natural ingredient extracted from grape seeds, is rich in powerful antioxidants such as proanthocyanidins and polyphenols and is widely used in skincare, healthcare, and pharmaceutical products. However, the active ingredients in grape seed extract are chemically unstable and easily degraded by factors such as light, heat, oxygen, and metal ions. This results in significant loss of the active ingredients during processing or use, significantly reducing their efficacy and preventing them from achieving their intended effects. To address this issue, some technologies have been developed to maintain the stability of grape seed extract through methods such as light-proof packaging and low-temperature storage, but these efforts are insufficient to meet the demands of skincare products that require room-temperature circulation. Summary of the Invention

[0003] The present invention provides a skin care composition containing grape seed extract, a preparation method and application thereof, in order to solve the technical problem that the efficacy of skin care products is reduced due to instability of grape seed extract.

[0004] In order to solve the above technical problems, in a first aspect, the present invention provides a skin care composition containing grape seed extract, comprising the following components in percentage by mass:

[0005] Grape seed component embedded body 5% to 10%;

[0006] Oil phase 5% to 20%;

[0007] Emulsifier 1% to 10%;

[0008] Aqueous phase 70% to 85%;

[0009] Among them, the grape seed component embedded body is a grape seed component embedded body obtained by using β-cyclodextrin to embed grape seed extract to form an embedded body, and then adding trehalose and mannitol; the skin care composition is a skin care composition obtained by embedding the grape seed component embedded body based on nanoemulsification embedding technology.

[0010] In some embodiments, the grape seed component embedded body is composed of the following raw materials in parts by weight:

[0011] 1-2 parts grape seed extract;

[0012] 2 to 5 parts of β-cyclodextrin;

[0013] 1 to 2 parts of trehalose;

[0014] Mannitol 0.1 to 1 part.

[0015] In some embodiments, the method for preparing the grape seed component embedded body includes:

[0016] Dissolve β-cyclodextrin in an appropriate amount of deionized water at 50°C to 60°C and stir until it is completely dissolved to form a cyclodextrin solution;

[0017] Grape seed extract was slowly added dropwise to the cyclodextrin solution, stirred at 40°C to 50°C in the dark for 1 to 2 hours, then ultrasonically treated at 200W for 5 to 20 minutes, and then centrifuged to remove the unincluded precipitate to obtain the embedded body;

[0018] Trehalose and mannitol were added to the embedding body, stirred evenly, and quickly cooled to below 25° C., and then the pH was adjusted to 6.0-7.0, and 0.05% vitamin E was added and stirred evenly to obtain a grape seed component embedding body.

[0019] In some embodiments, the method for extracting the grape seed extract comprises:

[0020] The grape seeds were crushed into 80-100 mesh sizes, and the grape seeds were ultrasonically extracted with a 50%-60% ethanol aqueous solution at a solid-liquid ratio of 1:10 at 40°C-50°C for 2-3 hours. The supernatant was then centrifuged at 4000 rpm for 10-20 minutes, and concentrated under reduced pressure to a solid content of not less than 30%. The supernatant was then purified by adding macroporous resin AB-8 to obtain a grape seed extract.

[0021] In some embodiments, the oil phase consists of grape seed oil and medium-chain triglycerides; the aqueous phase consists of sodium hyaluronate, glycerol, EDTA-2Na and deionized water; and the emulsifier includes one or more of lecithin, Tween-80, Span80, gum arabic and gelatin.

[0022] In some embodiments, the method for preparing the skin care composition comprises:

[0023] Grape seed oil and medium chain triglycerides are mixed uniformly in a mass ratio of 7:3 in a water bath at 60°C to form an oil phase, and then grape seed component embedding bodies and emulsifiers are added to obtain a mixed oil phase;

[0024] Sodium hyaluronate, glycerol and EDTA-2Na were dissolved in deionized water to form an aqueous phase;

[0025] Slowly pour the mixed oil phase into the water phase at 50°C to 60°C, and high-speed shear at 5000rpm to 10000rpm for 5min to 10min to obtain a crude emulsion;

[0026] The crude emulsion was circulated through a high-pressure homogenizer at a temperature below 40° C. and a pressure of 800 bar to 1000 bar for three times to obtain a nanoemulsion;

[0027] Sodium alginate was added to the nanoemulsion, and after magnetic stirring for 30 minutes, a 1% calcium chloride solution was added dropwise, and the mixture was allowed to stand at 4° C. for 2 hours to obtain a skin care composition.

[0028] The present invention also provides a use of the skin care composition containing grape seed extract as described above in the preparation of skin care products, wherein the skin care products include creams, lotions, gels, lotions, essences or facial masks.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] The grape seed extract-containing skin care composition of the present invention utilizes nanoemulsification encapsulation technology and a dual encapsulation system to synergize and enhance the stability and transdermal absorption efficiency of the active ingredients. Specifically, β-cyclodextrin encapsulates the grape seed extract to form a molecular-level inclusion structure. Combined with the vitrification protection of trehalose and mannitol, this increases the retention rate of polyphenols to over 90% under high temperatures (40°C) and UV irradiation. Nanoemulsification technology disperses the encapsulated body into nanoscale emulsion particles, significantly improving transdermal permeability compared to conventional emulsions. The dual encapsulation structure (cyclodextrin + nanoemulsion) achieves long-lasting sustained release of the active ingredients, achieving a long-lasting effect while reducing irritation, making it suitable for sensitive skin. DETAILED DESCRIPTION

[0031] To make the above-mentioned objects, features, and advantages of the present invention more readily apparent, specific embodiments of the present invention are described in detail below. The following description sets forth numerous specific details to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways than those described herein, and those skilled in the art may make similar modifications without departing from the scope of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0032] As used herein, the term "prepared from" is synonymous with "comprising." As used herein, the terms "comprising," "including," "having," "containing," or any other variations thereof, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises the listed elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

[0033] When amount, concentration or other value or parameter is represented with range, preferred range or the range that a series of upper preferred value and lower preferred value limit are expressed, this should be understood as specifically disclosing all ranges formed by any pairing of any range upper limit or preferred value and any range lower limit or preferred value, no matter whether this range is disclosed separately.For example, when disclosing scope "1 to 5", described scope should be interpreted as including scope "1 to 4", "1 to 3", "1 to 2", "1 to 2 and 4 to 5", "1 to 3 and 5" etc.When numerical range is described in this article, unless otherwise stated, otherwise this scope is intended to include its end value and all integers and fractions within this range.

[0034] In addition, the indefinite articles "a" and "an" before the elements or components of the present invention do not limit the quantity requirement (i.e., the number of times the elements or components appear). Therefore, "a" or "an" should be interpreted as including one or at least one, and elements or components in the singular also include plural forms, unless the number is obviously intended to be singular.

[0035] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which the present invention pertains. The terms used herein in the specification of the present invention are for the purpose of describing specific embodiments only and are not intended to limit the present invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0036] Preparation Example 1 of Grape Seed Extract:

[0037] Grape seeds were crushed into 100 mesh, and the grape seeds were ultrasonically extracted with a 50% ethanol aqueous solution at a solid-liquid ratio of 1:10 at 50°C for 2 hours. The supernatant was then centrifuged at 4000 rpm for 10 minutes, and concentrated under reduced pressure to a solid content of not less than 30%. The supernatant was then added with macroporous resin AB-8 for purification to obtain a grape seed extract.

[0038] Preparation Example 2 of Grape Seed Extract:

[0039] Grape seeds were crushed into 80 mesh pieces, and the grape seeds were ultrasonically extracted with a 60% ethanol aqueous solution at a solid-liquid ratio of 1:10 at 40°C for 3 hours. The supernatant was then centrifuged at 4000 rpm for 20 minutes, and concentrated under reduced pressure to a solid content of not less than 30%. The supernatant was then added with macroporous resin AB-8 for purification to obtain a grape seed extract.

[0040] Preparation Example 1 of Grape Seed Component Embedded Body:

[0041] The grape seed component embedded body is composed of the following raw materials in parts by mass: 1 part of the grape seed extract of Preparation Example 1 of the grape seed extract; 2 parts of β-cyclodextrin; 1 part of trehalose; 0.1 part of mannitol;

[0042] β-cyclodextrin was dissolved in an appropriate amount of deionized water at 50°C and stirred until completely dissolved to form a cyclodextrin solution; the grape seed extract of the grape seed extract preparation example was slowly added dropwise to the cyclodextrin solution, stirred at 40°C in the dark for 2 hours, and then ultrasonically treated at 200W for 5 minutes, and then centrifuged to remove unincluded precipitates to obtain an embedded body;

[0043] Trehalose and mannitol were added to the embedding body, stirred evenly, and quickly cooled to below 25° C., and then the pH was adjusted to 6.0-7.0, and 0.05% vitamin E was added and stirred evenly to obtain a grape seed component embedding body.

[0044] Preparation Example 2 of Grape Seed Component Embedded Body:

[0045] The grape seed component embedded body is composed of the following raw materials in parts by mass: 2 parts of the grape seed extract of Preparation Example 2 of the grape seed extract; 5 parts of β-cyclodextrin; 2 parts of trehalose; 1 part of mannitol;

[0046] Dissolve β-cyclodextrin in an appropriate amount of deionized water at 60°C and stir until it is completely dissolved to form a cyclodextrin solution;

[0047] The grape seed extract of Preparation Example 1 was slowly added dropwise to the cyclodextrin solution, stirred at 50°C in the dark for 1 to 2 hours, and then ultrasonically treated at 200W for 20 minutes. The unincluded precipitate was removed by centrifugation to obtain an embedded body;

[0048] Trehalose and mannitol were added to the embedding body, stirred evenly, and quickly cooled to below 25° C., and then the pH was adjusted to 6.0-7.0, and 0.05% vitamin E was added and stirred evenly to obtain a grape seed component embedding body.

[0049] Example 1

[0050] The skin care composition containing grape seed extract comprises the following components in percentage by mass:

[0051] Grape seed component embedded body 5%;

[0052] Grapeseed oil and medium-chain triglycerides 10% in a mass ratio of 7:3;

[0053] Lecithin 1%;

[0054] The aqueous phase is 84%, specifically composed of 1% sodium hyaluronate, 3% glycerol, 0.1% EDTA-2Na and the balance deionized water;

[0055] The preparation method of the skin care composition comprises:

[0056] Grape seed oil and medium chain triglycerides are mixed uniformly in a mass ratio of 7:3 in a water bath at 60°C to form an oil phase, and then grape seed component embedding bodies and emulsifiers are added to obtain a mixed oil phase;

[0057] Sodium hyaluronate, glycerol and EDTA-2Na were dissolved in deionized water to form an aqueous phase;

[0058] At 50°C, the mixed oil phase was slowly poured into the water phase and high-speed sheared at 5000 rpm for 10 min to obtain a crude emulsion;

[0059] The crude emulsion was circulated through a high-pressure homogenizer at below 40°C and 800 bar pressure for three times to obtain a nanoemulsion;

[0060] Sodium alginate was added to the nanoemulsion, and after magnetic stirring for 30 minutes, a 1% calcium chloride solution was added dropwise, and the mixture was allowed to stand at 4° C. for 2 hours to obtain a skin care composition.

[0061] Example 2

[0062] The skin care composition containing grape seed extract comprises the following components in percentage by mass:

[0063] Grape seed component embedded body 10%;

[0064] Grapeseed oil and medium-chain triglycerides 10% in a mass ratio of 7:3;

[0065] Lecithin 10%;

[0066] The aqueous phase is 70%, specifically composed of 1% sodium hyaluronate, 3% glycerin, 0.1% EDTA-2Na and the balance deionized water; the preparation method of the skin care composition comprises:

[0067] Grape seed oil and medium chain triglycerides are mixed uniformly in a mass ratio of 7:3 in a water bath at 60°C to form an oil phase, and then grape seed component embedding bodies and emulsifiers are added to obtain a mixed oil phase;

[0068] Sodium hyaluronate, glycerol and EDTA-2Na were dissolved in deionized water to form an aqueous phase;

[0069] At 560°C, the mixed oil phase was slowly poured into the water phase and high-speed sheared at 10,000 rpm for 10 min to obtain a crude emulsion;

[0070] The crude emulsion was circulated through a high-pressure homogenizer at below 40°C and 1000 bar pressure for three times to obtain a nanoemulsion;

[0071] Sodium alginate was added to the nanoemulsion, and after magnetic stirring for 30 minutes, a 1% calcium chloride solution was added dropwise, and the mixture was allowed to stand at 4° C. for 2 hours to obtain a skin care composition.

[0072] Comparative Example 1

[0073] Compared with Example 1, Comparative Example 1 uses the grape seed extract of Preparation Example 1 of the grape seed extract instead of the grape seed component embedded body.

[0074] Comparative Example 2

[0075] Compared with Example 1, Comparative Example 2 no longer added trehalose and mannitol.

[0076] Comparative Example 3

[0077] Compared with Example 1, in Comparative Example 3, the grape seed component embedded body was prepared without rapid cooling and without adding vitamin E.

[0078] Comparative Example 4

[0079] Compared with Example 1, in Comparative Example 4, after adding trehalose and mannitol, freeze-drying technology was used to replace the subsequent steps to prepare freeze-dried powder.

[0080] Comparative Example 5

[0081] Compared with Example 1, Comparative Example 5 adopts a traditional mixing method instead of the preparation method of the skin care composition.

[0082] Comparative Example 6

[0083] Compared with Example 1, Comparative Example 6 uses the nanoemulsion as the skin care composition.

[0084] 1. Stability test

[0085] Test Method: Samples were placed in an oxygen-tight Petri dish and exposed to natural light for 7 days to measure photostability, and the absorbance decrease rate was calculated. Thermal stability was measured by placing the sample in a transparent Petri dish and placing it in an 80°C oven for 20 minutes, and the absorbance decrease rate was calculated. Absorbance decrease rate = (absorbance before test - absorbance after test) / absorbance before test × 100%. The test results are shown in Table 1.

[0086] 2. Anti-aging test

[0087] Test method: 50 pregnant C. elegans were placed on NGM plates, inoculated with Escherichia coli (OP50 strain), and laid for 3 hours. The NGM plates containing C. elegans eggs were cultured at 20°C until the offspring of C. elegans reached L4 larvae. The L4 larvae of C. elegans were divided into 9 groups, each with 100 larvae, of which 1 group was a blank control group and 8 groups were test groups. On the 0th day of the test, the test groups were cultured on NGM plates containing 1.5 mg / mL of sample; the control group was cultured on an NGM plate without sample; C. elegans that could not move were marked as dead; the larvae were counted every day, and the test was repeated 3 times for each group; the average lifespan of C. elegans in each group was calculated; the test results are shown in Table 1 below.

[0088]

[0089]

[0090] As can be seen from the above table, the photostability absorbance decrease rate of the skin care composition of the present invention is less than 3%, the thermal stability absorbance decrease rate is less than 9%, the average lifespan of Caenorhabditis elegans is greater than 30 days, and the photostability, thermal stability and anti-aging performance are all better than those of Comparative Examples 1 to 6, indicating that the preparation method of the present invention can effectively protect the grape seed extract in the skin care composition.

[0091] The technical features of the above-mentioned embodiments can be combined arbitrarily. In order to make the description concise, not all possible combinations of the technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0092] The specific embodiments described above further illustrate the objectives, technical solutions, and beneficial effects of the present invention. It should be understood that the above descriptions are merely specific embodiments of the present invention and are not intended to limit the scope of protection of the present invention. In particular, it should be noted that any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included within the scope of protection of the present invention for those skilled in the art.

Claims

1. A skin care composition containing grape seed extract, characterized in that: It includes the following components in percentage by mass: Grape seed component embedded body 5% to 10%; Oil phase 5% to 20%; Emulsifier 1% to 10%; Aqueous phase 70% to 85%; Among them, the grape seed component embedded body is a grape seed component embedded body obtained by using β-cyclodextrin to embed grape seed extract to form an embedded body, and then adding trehalose and mannitol; the skin care composition is a skin care composition obtained by embedding the grape seed component embedded body based on nanoemulsification embedding technology.

2. The skin care composition containing grape seed extract according to claim 1, wherein The grape seed component embedded body is composed of the following raw materials in parts by mass: 1-2 parts grape seed extract; 2 to 5 parts of β-cyclodextrin; 1 to 2 parts of trehalose; Mannitol 0.1 to 1 part.

3. The skin care composition containing grape seed extract according to claim 1, wherein The preparation method of the grape seed component embedded body comprises: Dissolve β-cyclodextrin in an appropriate amount of deionized water at 50°C to 60°C and stir until it is completely dissolved to form a cyclodextrin solution; Grape seed extract was slowly added dropwise to the cyclodextrin solution, stirred at 40°C to 50°C in the dark for 1 to 2 hours, then ultrasonically treated at 200W for 5 to 20 minutes, and then centrifuged to remove the unincluded precipitate to obtain the embedded body; Trehalose and mannitol were added to the embedding body, stirred evenly, and quickly cooled to below 25° C., and then the pH was adjusted to 6.0-7.0, and 0.05% vitamin E was added and stirred evenly to obtain a grape seed component embedding body.

4. The skin care composition containing grape seed extract according to claim 1, wherein The extraction method of the grape seed extract comprises: The grape seeds were crushed into 80-100 mesh sizes, and the grape seeds were ultrasonically extracted with a 50%-60% ethanol aqueous solution at a solid-liquid ratio of 1:10 at 40°C-50°C for 2-3 hours. The supernatant was then centrifuged at 4000 rpm for 10-20 minutes, and concentrated under reduced pressure to a solid content of not less than 30%. The supernatant was then purified by adding macroporous resin AB-8 to obtain a grape seed extract.

5. The skin care composition containing grape seed extract according to claim 1, wherein The oil phase consists of grape seed oil and medium-chain triglycerides; the aqueous phase consists of sodium hyaluronate, glycerol, EDTA-2Na and deionized water; and the emulsifier includes one or more of lecithin, Tween-80, Span80, gum arabic and gelatin.

6. The skin care composition containing grape seed extract according to claim 1, wherein The preparation method of the skin care composition comprises: Grape seed oil and medium chain triglycerides are mixed uniformly in a mass ratio of 7:3 in a water bath at 60°C to form an oil phase, and then grape seed component embedding bodies and emulsifiers are added to obtain a mixed oil phase; Sodium hyaluronate, glycerol and EDTA-2Na were dissolved in deionized water to form an aqueous phase; Slowly pour the mixed oil phase into the water phase at 50°C to 60°C, and high-speed shear at 5000rpm to 10000rpm for 5min to 10min to obtain a crude emulsion; The crude emulsion was circulated through a high-pressure homogenizer at a temperature below 40° C. and a pressure of 800 bar to 1000 bar for three times to obtain a nanoemulsion; Sodium alginate was added to the nanoemulsion, and after magnetic stirring for 30 minutes, a 1% calcium chloride solution was added dropwise, and the mixture was allowed to stand at 4° C. for 2 hours to obtain a skin care composition.

7. Use of the skin care composition containing grape seed extract according to any one of claims 1 to 6 in the preparation of skin care products, characterized in that: The skin care products include creams, lotions, gels, water solutions, essences or facial masks.

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