Pseudo-ginseng flower and ampelopsis grossedentata self-assembled nano-particles as well as preparation method and application thereof

Abstract

The invention relates to sanchi flower and ampelopsis grossedentata self-assembled nanoparticles as well as a preparation method and application thereof. The preparation method of the pseudo-ginseng flower and ampelopsis grossedentata self-assembled nanoparticles comprises the following steps: mixing and crushing pseudo-ginseng flower and ampelopsis grossedentata to obtain mixed powder; soaking the mixed powder with hot water, then adding hydrolase for enzymolysis, and filtering to obtain enzymolysis filtrate; adding water into the enzymolysis filtrate, heating and pressurizing for water extraction to obtain an extracting solution; concentrating the extracting solution under reduced pressure, and freeze-drying to obtain the pseudo-ginseng flower and vine tea self-assembled nano-particles. The invention further provides the pseudo-ginseng flower and vine tea self-assembled nano-particles and application thereof in wine. The novel panax notoginseng flower and vine tea composite self-assembled nanoparticles and the related application thereof are developed by skillfully combining the two natural plant resources of panax notoginseng flower and vine tea, so that the application field of panax notoginseng flower and vine tea is widened.

Description

Technical Field

[0001] The present invention relates to the technical field of notoginseng flower applications, and in particular to notoginseng flower and rattan tea self-assembled nanoparticles, a preparation method and applications thereof. Background Art

[0002] Panax notoginseng, also known as Tianqi (Five-leaved Psoralea corylifolia) and Jinbuhuan (Ginseng), is a member of the genus Panax, in the Araliaceae family. It is a traditional and valuable Chinese medicinal herb with a long history in my country. It is primarily distributed in Yunnan and Guangxi, with Wenshan Prefecture being the authentic production area. Its roots and rhizomes are known for their properties, such as dispersing blood stasis, stopping bleeding, reducing swelling, and alleviating pain, and are widely used to treat a variety of ailments. In recent years, with the continuous advancement of research on Panax notoginseng, its medicinal value has been further explored and recognized.

[0003] As one of the main components of the medicinal plant Panax notoginseng, Panax notoginseng flower also possesses high medicinal value. It possesses a cooling nature, a sweet taste, and is rich in saponins, such as ginsenosides Rb1, Rb2, Rb3, and Rc, as well as various amino acids, polysaccharides, proteins, and organic acids. Scientific research has shown that Panax notoginseng flower has the effects of clearing heat and promoting fluid production, calming the liver and suppressing yang, and promoting blood circulation and removing blood stasis. It can be used to treat symptoms such as dizziness, vertigo, and tinnitus. It also dilates blood vessels, lowers blood pressure, increases myocardial oxygen supply, and enhances immunity. In recent years, Panax notoginseng flower has been included in the catalog of local specialty food resources, paving the way for the research and development of medicinal and edible products.

[0004] Ampelopsis grossedentata, also known as berry tea, mountain sweet tea, field tea, and Dragon Boat Festival tea, is a woody vine belonging to the genus Ampelopsis in the family Vitaceae. It is primarily found south of the Yangtze River in my country, including Hunan, Hubei, Guangxi, Guangdong, Jiangxi, Fujian, Yunnan, and Guizhou. The stems and leaves of Ampelopsis grossedentata can be processed into a tea substitute, a practice with a long history of at least 700 to 1,000 years.

[0005] Tengcha (Ganoderma lucidum) has a unique growth pattern, creeping like a vine along rocky cliffs. Its young leaves and stems are widely used for medicinal and health-promoting purposes. The main active ingredients in tengcha are flavonoids, particularly dihydromyricetin (DMY), which exhibit various biological activities, including antioxidant, anti-inflammatory, antibacterial, hypoglycemic, hypolipidemic, and anti-tumor properties. Tengcha also contains polysaccharides, polyphenols, organic acids, steroids, volatile oils, amino acids, and trace elements. As a natural plant resource with diverse medicinal and health-promoting properties, tengcha has broad application prospects and development potential. Summary of the Invention

[0006] In view of this, the purpose of the present invention is to provide a kind of Panax notoginseng flower and rattan tea self-assembled nanoparticles, preparation method and application, so as to develop a new type of Panax notoginseng flower and rattan tea composite self-assembled nanoparticles and related applications by cleverly combining the two natural plant resources of Panax notoginseng flower and rattan tea, thereby expanding the application field of Panax notoginseng flower and rattan tea.

[0007] In order to achieve the above object, the technical solution adopted by the present invention is as follows: A method for preparing self-assembled nanoparticles of Panax notoginseng flower and rattan tea comprises the following steps: S1. Mix and grind the notoginseng flower and rattan tea to obtain a mixed powder; S2, soaking the mixed powder in hot water, then adding hydrolase to hydrolyze, filtering, and obtaining an enzymatic filtrate; S3, adding water to the enzymatic hydrolysis filtrate, heating and pressurizing the water to obtain an extract; S4. The extract is concentrated under reduced pressure and freeze-dried to obtain self-assembled nanoparticles of Panax notoginseng flower and Ampelopsis pilosula.

[0008] According to the above technical means, by adopting a mixed pulverization method to grind Panax notoginseng flowers (containing saponins and polysaccharides) and rattan tea (rich in dihydromyricetin and flavonoids), the release of active ingredients is effectively improved; by enzymatic hydrolysis of the pulverized mixed powder, the degradation of plant cell wall polysaccharides is further achieved, thereby increasing the dissolution rate of small molecule active ingredients (such as saponins), providing precursor substances for subsequent self-assembly; by extracting the enzymatic hydrolysis filtrate under high temperature and high pressure conditions, the interaction between molecules is promoted, so that the hydrophobic aglycones of Panax notoginseng saponins (such as ginsenoside Rg1) and the phenolic hydroxyl groups of rattan tea flavonoids (such as dihydromyricetin) form complexes through hydrophobic interaction and hydrogen bonding, and the polysaccharide chains wrap the hydrophobic core through electrostatic interaction or molecular entanglement to form a micelle-like structure, thereby ensuring the formation of self-assembled particles; by treating the extract by reduced pressure concentration, the intermolecular force is further enhanced to form a stable self-assembled structure; by treating the concentrate by freeze-drying, the porous structure is effectively retained, and the redispersibility and stability of the nanoparticles are improved.

[0009] During the experiment, it was found that the mixed extract of Panax notoginseng flower and Ampelopsis pilosula (Gentiana gracile leaves) was significantly different from that of each extract alone. In particular, Ampelopsis pilosula extract became more stable, and the precipitate was significantly reduced after low temperature treatment (see Figures 1 to 3 ).

[0010] Among them, low-temperature treatment means that because dihydromyricetin (DMY, white crystals) in rattan tea is sensitive to temperature, DMY in rattan tea will precipitate quickly under low temperature conditions (around 4°C), and will also precipitate quickly if left at room temperature (around 25°C) for a slightly longer time, thereby reducing the medicinal value of the product. This application uses a mixed extraction of Panax notoginseng flower and rattan tea (Apis ampelopsis radiata leaves) to cause the Panax notoginseng flower and rattan tea to self-assemble, so that DMY will not precipitate regardless of the low temperature of 4°C, room temperature of 25°C, or high temperature of 100°C. DMY is retained in the extract, thereby improving the stability of the rattan tea extract and enhancing the medicinal value of the self-assembled nanoparticles of Panax notoginseng flower and rattan tea.

[0011] Further research confirmed that this is due to the natural self-assembly effect of the saponins in Panax notoginseng flower and dihydromyricetin in rattan tea (see Figure 4 、 Figure 5 and Figure 7 This improved the stability of the dihydromyricetin solution, while also enhancing the water solubility of Panax notoginseng flower saponins and the sustained release of volatile oils (as determined by nasal administration over three consecutive days). Therefore, the combined extraction of Panax notoginseng flower and vine tea not only increased the bioavailability of active ingredients and enhanced their specific pharmacological activity, but also improved product stability and shelf life, achieving a synergistic effect.

[0012] Preferably, the mass ratio of the Panax notoginseng flower to the rattan tea is 1:6 to 6:1.

[0013] By precisely controlling the mass ratio of Panax notoginseng flower and vine tea, natural self-assembled particles SAN of Panax notoginseng flower and vine tea were successfully formed.

[0014] Experimental research has found that the correct ratio of Panax notoginseng flower to vine tea is a key factor in the process. If the ratio is incorrect, self-assembled particles will be difficult to form or may result in non-specific self-assembled particles. Specifically, if the mass ratio of Panax notoginseng flower to vine tea is less than 1:6, DMY precipitates will remain in the extract, resulting in DMY loss. If the mass ratio exceeds 6:1, excessive saponin molecules will "grab" the DMY, causing instability in the self-assembled structure.

[0015] Preferably, the volume ratio of the enzymatic hydrolysis filtrate to water is 1:15 to 1:20.

[0016] Preferably, the water is purified water.

[0017] By precisely controlling the ratio of enzymatic filtrate to water, as well as the extraction pressure, the extraction efficiency of Panax notoginseng flower and vine tea is ensured.

[0018] The second key technology is precisely controlling the ratio of enzymatic filtrate to water. Specifically, a material-to-liquid ratio below 1:15 will affect the extraction rate of DMY and ginsenoside Rb3, thereby affecting the yield of self-assembled particles. A material-to-liquid ratio above 1:20 will exceed the minimum critical concentration of the self-assembly system, making it difficult to form a complete and stable self-assembled particle system.

[0019] Preferably, the hydrolase is at least one selected from cellulase, pectinase and α-amylase.

[0020] Preferably, the added mass of the hydrolase is 1-2% of the mixed powder.

[0021] Preferably, the temperature of the hot water for soaking the mixed powder is 50-65°C.

[0022] Preferably, the temperature of the hot water for soaking the mixed powder is 50°C.

[0023] Preferably, the temperature of the heated pressurized water extraction is 90-100° C., and the pressure is at least 0.15 MPa.

[0024] Preferably, the heated pressurized water extraction is performed in a sealed environment, with a specific material-liquid ratio, purified water as the extraction medium, and a sealed extraction process to ensure that a specific pressure is reached in the extraction tank.

[0025] Preferably, the time of the heated pressurized water extraction is 2 to 2.5 hours.

[0026] Preferably, the pressure of the reduced pressure concentration is 60 kPa.

[0027] Preferably, the reduced pressure concentration is carried out under sealed conditions.

[0028] Preferably, the extract is concentrated under reduced pressure to 1.5 times the weight of the mixed powder.

[0029] This process, a core step, employs sealed concentration, specific pressure values, and a specific evaporation rate or concentration ratio to ensure that the extract is concentrated within the specified timeframe, effectively guaranteeing the SAN granule yield, the color and aroma of the final product, and the shelf life of the Panax notoginseng flower SAN. If the concentration time is too long, on the one hand, excessive loss of the Panax notoginseng flower volatile oil will occur, affecting the fragrance of products developed using it as a raw material; on the other hand, gelatinization of the starch component and carbonization of the polysaccharide components will affect the color of the raw material. On the other hand, if the time is too short, the moisture content will be too high, increasing the cost of freeze-drying.

[0030] Preferably, the freeze-drying temperature is -40°C.

[0031] Preferably, the Panax notoginseng flower is selected from the flower buds of Panax notoginseng that are at least three years old, the water content of the Panax notoginseng flower is not higher than 13%, and the total saponin content of the Panax notoginseng flower is not lower than 15%.

[0032] Preferably, the water content of the rattan tea is not higher than 13%, the degree of mildew is above 40%, and the content of dihydromyricetin is not lower than 20%.

[0033] The present invention also provides self-assembled nanoparticles of Panax notoginseng flower and rattan tea prepared by the preparation method of the present invention.

[0034] The present invention also provides an application of the notoginseng flower and rattan tea self-assembled nanoparticles prepared by the preparation method in wine.

[0035] Preferably, the notoginseng flower and rattan tea self-assembled nanoparticles are added to wine to obtain notoginseng flower SAN wine.

[0036] Experimental research has demonstrated that the use of Panax notoginseng flower SAN in blending dew wine fully unleashes the benefits of Panax notoginseng flower saponins and dihydromyricetin from rattan tea, resulting in an excellent taste and significant potential for widespread application. The formula is formulated based on five key factors: color, aroma, flavor, form, and efficacy. The color is a yellowish hue formed by the combination of Panax notoginseng flower SAN and ethanol; the aroma is primarily derived from the volatile oils formed by the fusion of Panax notoginseng flower and rattan tea; the flavor is primarily derived from the slightly bitter taste of saponins and the sweet aftertaste of dihydromyricetin; the form is primarily derived from the translucent texture of saponins, dihydromyricetin, and ethanol; and the efficacy is primarily due to the protective effects of Panax notoginseng flower saponins and dihydromyricetin on liver function.

[0037] Preferably, the preparation method of the Panax notoginseng flower SAN wine comprises the following steps: Add lotus leaves to water, boil for 1 hour, and add the self-assembled nanoparticles of Panax notoginseng flower and Ampelopsis ulmoides after cooling to obtain a desensitizing solution; The base wine, wine materials and alcohol-lowering liquid are mixed, and then placed at -40°C for 15 days, then returned to the temperature of 10°C, filtered, and the Panax notoginseng flower SAN wine is obtained.

[0038] Preferably, the base wine includes wheat wine with an alcohol content of not less than 50° and sauce-flavor liquor with an alcohol content of not less than 53°.

[0039] Preferably, the wine material includes edible wine softener and edible glycerin, the addition ratio of the edible wine softener is 0.5%, and the addition ratio of the edible glycerin is 1.0%.

[0040] Among them, the purpose of adding wine is to increase the texture and age of the wine.

[0041] Preferably, the notoginseng flower and vine tea self-assembled nanoparticles are added after cooling to below 40°C.

[0042] Preferably, the filtration includes three rounds of filtration, the first round of filtration is centrifugal filtration at a speed of not less than 4000 r / min, the second round of filtration uses diatomaceous earth filtration, and the third round of filtration uses membrane cross-flow filtration with a flow rate of 1 t / h.

[0043] Beneficial effects of the present invention: 1) The present invention's method for preparing self-assembled nanoparticles of Panax notoginseng flower and rattan tea utilizes a clever combination of saponin-rich Panax notoginseng flower and dihydromyricetin-rich rattan tea as raw materials. Through pulverization, enzymatic hydrolysis, high-temperature and high-pressure extraction, concentration, and freeze-drying, the resulting nanoparticles are successfully prepared. This method boasts the advantages of ease of operation, high extraction efficiency, environmental friendliness, strong controllability, low cost, and excellent product stability. The resulting nanoparticles not only retain the unique efficacy of the raw materials but also exhibit superior bioavailability and stability due to nanoscale effects. This provides a solid foundation for the subsequent development of pharmaceuticals, health products, and functional foods, and possesses significant potential for industrial application in the field of Panax notoginseng flower applications.

[0044] 2) The present invention utilizes the self-assembled nanoparticles of Panax notoginseng flower and rattan tea in wine. By innovatively applying these nanoparticles to blended liquor, the efficacy of Panax notoginseng flower saponins and rattan tea dihydromyricetin is fully utilized, while also providing an excellent taste. The resulting Panax notoginseng flower SAN liquor combines the aroma of volatile oils from Panax notoginseng flower and rattan tea, the slightly bitter taste of saponins, and the sweet aftertaste of dihydromyricetin, while also emphasizing the liver-protecting effects of Panax notoginseng flower saponins and dihydromyricetin. This allows consumers to enjoy not only a delicious taste but also the health benefits of Panax notoginseng flower and rattan tea, fully satisfying the modern consumer's pursuit of a healthy lifestyle. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] Figure 1 This is a physical comparison diagram of the mixed filtrate obtained at the end of the extraction; Figure 2 This is a comparison photo of the mixed filtrate obtained after being stored at 4°C overnight (front); Figure 3 This is a comparison photo of the mixed filtrate obtained after being stored at 4°C overnight (back); Figure 4 This is the result graph of dihydromyricetin content in mixed filtrates obtained by extracting Panax notoginseng flower and rattan tea at different mass ratios; Figure 5 This is the result graph of ginsenoside Rb3 content in mixed filtrates obtained by extracting Panax notoginseng flower and rattan tea at different mass ratios; Figure 6 This is a physical picture of the Panax notoginseng flower SAN preparation prepared in Example 1; Figure 7 This is a laser confocal microscopic image of the Panax notoginseng flower SAN preparation prepared in Example 1; Figure 8 is the extraction rate when the ratio of Panax notoginseng flower and rattan tea is reduced in different proportions; Figure 9 is the extraction rate when Panax notoginseng flower and rattan tea are added in different proportions; Figure 10 It is the extraction rate when Panax notoginseng flower and rattan tea are added in equal proportions. DETAILED DESCRIPTION

[0046] The following will describe the embodiments of the present invention with reference to preferred embodiments. Those skilled in the art will readily appreciate the other advantages and benefits of the present invention from the disclosure herein. The present invention may also be implemented or applied through various other specific embodiments, and the details in this specification may be modified or altered based on different viewpoints and applications without departing from the spirit of the present invention. It should be understood that the preferred embodiments are intended only to illustrate the present invention and are not intended to limit the scope of protection of the present invention.

[0047] Example 1 A method for preparing self-assembled nanoparticles of Panax notoginseng flower and rattan tea comprises the following steps: S1, mixing and grinding Panax notoginseng flower with a water content of not more than 10% and a total saponin content of not less than 15% and rattan tea with a mildew degree of more than 40%, a water content of not more than 13% and a dihydromyricetin content of not less than 20% in a mass ratio of 1:1 to obtain a mixed powder; S2. Soak 2 kg of the mixed powder in 50° C. hot water, then add 30 g of cellulase for full enzymatic hydrolysis, and filter to obtain an enzymatic filtrate; wherein, the viscosity of the solution is regularly measured using a viscometer, and the viscosity is reduced to 50% to 70% of the initial value and there is no obvious turbidity, indicating that full enzymatic hydrolysis has been carried out; S3. The enzymatic filtrate and water are mixed in a volume ratio of 1:15, heated by hot oil, and heated and pressurized water is extracted for 2 hours in a sealed environment at a temperature of 95°C and a pressure of 0.15 MPa. The extract is filtered through an 80-mesh sieve, and the filtrates are merged and mixed to obtain a mixed filtrate; wherein, extracting twice refers to: first, heating and pressurizing water is extracted for 2 hours in a sealed environment at a temperature of 95°C and a pressure of 0.15 MPa, filtering to obtain a filter residue and a first filtrate, then cooling the filter residue to room temperature and pressure, then heating it again to 95°C, and increasing the pressure to 0.15 MPa in a sealed environment for heating and pressurizing extraction for 2 hours, filtering to obtain a filter residue and a second filtrate, and then mixing the first filtrate and the second filtrate to obtain a mixed filtrate; S4. The mixed filtrate is concentrated under reduced pressure to 1.5 times the weight of the raw material, and then quickly put into a freeze dryer at a temperature of -40°C for freeze drying. Concentration and freezing need to be performed continuously to obtain self-assembled nanoparticles of Panax notoginseng flower and vine tea, namely Panax notoginseng flower SAN (self-assembled nanoparticles) preparation. The actual picture of the obtained Panax notoginseng flower SAN preparation is as follows: Figure 6 As shown. Figure 6 The analysis showed that the prepared Panax notoginseng flower SAN preparation was a brown powder.

[0048] Example 2 A method for preparing self-assembled nanoparticles of Panax notoginseng flower and rattan tea comprises the following steps: S1, mixing and grinding Panax notoginseng flower with a water content of not more than 10% and a total saponin content of not less than 15% and rattan tea with a mildew degree of more than 40%, a water content of not more than 13% and a dihydromyricetin content of not less than 20% in a mass ratio of 2:1, 3:1, 4:1, 5:1 and 6:1 respectively to obtain a mixed powder; S2. Soak 2 kg of the mixed powder in 50° C. hot water, then add 30 g of cellulase for full enzymatic hydrolysis, and filter to obtain an enzymatic filtrate; wherein, the viscosity of the solution is regularly measured using a viscometer, and the viscosity is reduced to 50% to 70% of the initial value and there is no obvious turbidity, indicating that full enzymatic hydrolysis has been carried out; S3. The enzymatic filtrate and water are mixed in a volume ratio of 1:15, heated by hot oil, and heated and pressurized water is extracted for 2 hours in a sealed environment at a temperature of 95°C and a pressure of 0.15 MPa. The extract is extracted twice to obtain an extract passed through an 80-mesh sieve, and the filtrates are merged and mixed to obtain a mixed filtrate; wherein, extracting twice refers to: first, heating and pressurizing water is extracted for 2 hours in a sealed environment at a temperature of 95°C and a pressure of 0.15 MPa, filtering to obtain a filter residue and a first filtrate, then cooling the filter residue to room temperature and pressure, then heating it again to 95°C, and increasing the pressure to 0.15 MPa in a sealed environment for heating and pressurizing extraction for 2 hours, filtering to obtain a filter residue and a second filtrate, and then mixing the first filtrate and the second filtrate to obtain a mixed filtrate; S4. The mixed filtrate is concentrated under reduced pressure to 1.5 times the weight of the raw material, and then quickly put into a freeze dryer at a temperature of -40°C for freeze drying. Concentration and freezing need to be performed continuously to obtain Panax notoginseng flower and vine tea self-assembled nanoparticles, namely Panax notoginseng flower SAN preparation.

[0049] Example 3 A method for preparing self-assembled nanoparticles of Panax notoginseng flower and rattan tea comprises the following steps: S1, mixing and grinding Panax notoginseng flower with a water content of not more than 10% and a total saponin content of not less than 15% and rattan tea with a mildew degree of more than 40%, a water content of not more than 13% and a dihydromyricetin content of not less than 20% in a mass ratio of 1:2, 1:3, 1:4, 1:5 and 1:6 respectively to obtain a mixed powder; S2. Soak 2 kg of the mixed powder in 50° C. hot water, then add 30 g of cellulase for full enzymatic hydrolysis, and filter to obtain an enzymatic filtrate; wherein, the viscosity of the solution is regularly measured using a viscometer, and the viscosity is reduced to 50% to 70% of the initial value and there is no obvious turbidity, indicating that full enzymatic hydrolysis has been carried out; S3. The enzymatic filtrate and water are mixed in a volume ratio of 1:15, heated by hot oil, and heated and pressurized water is extracted for 2 hours in a sealed environment at a temperature of 95°C and a pressure of 0.15 MPa. The extract is extracted twice to obtain an extract passed through an 80-mesh sieve, and the filtrates are merged and mixed to obtain a mixed filtrate; wherein, extracting twice refers to: first, heating and pressurizing water is extracted for 2 hours in a sealed environment at a temperature of 95°C and a pressure of 0.15 MPa, filtering to obtain a filter residue and a first filtrate, then cooling the filter residue to room temperature and pressure, then heating it again to 95°C, and increasing the pressure to 0.15 MPa in a sealed environment for heating and pressurizing extraction for 2 hours, filtering to obtain a filter residue and a second filtrate, and then mixing the first filtrate and the second filtrate to obtain a mixed filtrate; S4. The mixed filtrate is concentrated under reduced pressure to 1.5 times the weight of the raw material, and then quickly put into a freeze dryer at a temperature of -40°C for freeze drying. Concentration and freezing need to be performed continuously to obtain Panax notoginseng flower and vine tea self-assembled nanoparticles, namely Panax notoginseng flower SAN preparation.

[0050] Example 4 A method for preparing self-assembled nanoparticles of Panax notoginseng flower and rattan tea comprises the following steps: S1, mixing and grinding Panax notoginseng flower with a water content of not more than 10% and a total saponin content of not less than 15% and rattan tea with a mildew degree of more than 40%, a water content of not more than 13% and a dihydromyricetin content of not less than 20% in a mass ratio of 2:2, 3:3, 4:4, 5:5 and 6:6 respectively to obtain a mixed powder; S2. Soak 2 kg of the mixed powder in 50° C. hot water, then add 30 g of cellulase for full enzymatic hydrolysis, and filter to obtain an enzymatic filtrate; wherein, the viscosity of the solution is regularly measured using a viscometer, and the viscosity is reduced to 50% to 70% of the initial value and there is no obvious turbidity, indicating that full enzymatic hydrolysis has been carried out; S3. The enzymatic filtrate and water are mixed in a volume ratio of 1:15, heated by hot oil, and heated and pressurized water is extracted for 2 hours in a sealed environment at a temperature of 95°C and a pressure of 0.15 MPa. The extract is extracted twice to obtain an extract passed through an 80-mesh sieve, and the filtrates are merged and mixed to obtain a mixed filtrate; wherein, extracting twice refers to: first, heating and pressurizing water is extracted for 2 hours in a sealed environment at a temperature of 95°C and a pressure of 0.15 MPa, filtering to obtain a filter residue and a first filtrate, then cooling the filter residue to room temperature and pressure, then heating it again to 95°C, and increasing the pressure to 0.15 MPa in a sealed environment for heating and pressurizing extraction for 2 hours, filtering to obtain a filter residue and a second filtrate, and then mixing the first filtrate and the second filtrate to obtain a mixed filtrate; S4. The mixed filtrate is concentrated under reduced pressure to 1.5 times the weight of the raw material, and then quickly placed in a freeze dryer at a temperature of -40°C for freeze drying. Concentration and freezing must be performed continuously to obtain self-assembled nanoparticles of Panax notoginseng flower and vine tea, namely Panax notoginseng flower SAN preparation. Example 5 A method for preparing Panax notoginseng flower SAN wine comprises the following steps: S1. Mix lotus leaves and water in a mass ratio of 1:200, heat to boiling, and keep boiling for 1 hour. After cooling to below 40° C., add 15 g of the Panax notoginseng flower SAN preparation prepared in Example 1 to obtain a reduced-alcohol solution; S2. Wheat wine with an alcohol content of not less than 50° and Maotai-flavor liquor with an alcohol content of not less than 53° are used as base wines, and edible wine softener and edible glycerin are added as wine materials and alcohol-lowering liquid in sequence into a dissolving and preparing tank, stirred or circulated for more than 1 hour, the alcohol content is measured after the liquid is passed, and the alcohol content is adjusted to the required alcohol content to obtain Panax notoginseng flower SAN base wine, wherein the addition ratio of edible wine softener to the Panax notoginseng flower SAN base wine is 0.5%, the addition ratio of edible glycerin to the Panax notoginseng flower SAN base wine is 1.0%, and the base wine and alcohol-lowering liquid are calculated according to the required alcohol content; S3. Cool the Panax notoginseng flower SAN raw wine to -40°C within 12 hours, maintain the temperature for 15 days, then return the temperature to 10°C and perform centrifugal filtration at a speed of not less than 4000 r / min, then perform diatomaceous earth filtration and membrane cross-flow filtration in sequence at a filtration flow rate of 1 t / h to obtain Panax notoginseng flower SAN liquor.

[0051] Comparative Example 1 A method for preparing Panax notoginseng flower self-assembled nanoparticles comprises the following steps: S1. Grinding Panax notoginseng flower having a water content of not more than 10% and a total saponin content of not less than 15% to obtain Panax notoginseng flower powder; S2. Soak 2 kg of Panax notoginseng flower powder in 50° C. hot water, then add 30 g of cellulase for full enzymatic hydrolysis, and filter to obtain an enzymatic filtrate; wherein, the viscosity of the solution is regularly measured using a viscometer, and the viscosity is reduced to 50% to 70% of the initial value, and there is no obvious turbidity, indicating that full enzymatic hydrolysis has been carried out; S3. The enzymatic filtrate and water are mixed in a volume ratio of 1:15, heated by hot oil, and heated and pressurized water is extracted for 2 hours in a sealed environment at a temperature of 95°C and a pressure of 0.15 MPa. The extract is extracted twice to obtain an extract passed through an 80-mesh sieve, and the filtrates are merged and mixed to obtain a mixed filtrate; wherein, extracting twice refers to: first, heating and pressurizing water is extracted for 2 hours in a sealed environment at a temperature of 95°C and a pressure of 0.15 MPa, filtering to obtain a filter residue and a first filtrate, then cooling the filter residue to room temperature and pressure, then heating it again to 95°C, and increasing the pressure to 0.15 MPa in a sealed environment for heating and pressurizing extraction for 2 hours, filtering to obtain a filter residue and a second filtrate, and then mixing the first filtrate and the second filtrate to obtain a mixed filtrate; S4. The mixed filtrate is concentrated under reduced pressure to 1.5 times the weight of the raw material, and then quickly put into a freeze dryer at a temperature of -40°C for freeze drying. The concentration and freezing need to be performed continuously to obtain Panax notoginseng flower self-assembled nanoparticles.

[0052] Comparative Example 2 A method for preparing self-assembled nanoparticles of rattan tea comprises the following steps: S1, grinding rattan tea with a mildew degree of more than 40%, a water content of not more than 13% and a dihydromyricetin content of not less than 20% to obtain rattan tea powder; S2. Soak 2 kg of rattan tea powder in 50° C. hot water, then add 30 g of cellulase for full enzymatic hydrolysis, and filter to obtain an enzymatic filtrate; wherein, the viscosity of the solution is regularly measured using a viscometer, and the viscosity is reduced to 50% to 70% of the initial value, and there is no obvious turbidity, indicating that full enzymatic hydrolysis has been carried out; S3. The enzymatic filtrate and water are mixed in a volume ratio of 1:15, heated by hot oil, and heated and pressurized water is extracted for 2 hours in a sealed environment at a temperature of 95°C and a pressure of 0.15 MPa. The extract is extracted twice to obtain an extract passed through an 80-mesh sieve, and the filtrates are merged and mixed to obtain a mixed filtrate; wherein, extracting twice refers to: first, heating and pressurizing water is extracted for 2 hours in a sealed environment at a temperature of 95°C and a pressure of 0.15 MPa, filtering to obtain a filter residue and a first filtrate, then cooling the filter residue to room temperature and pressure, then heating it again to 95°C, and increasing the pressure to 0.15 MPa in a sealed environment for heating and pressurizing extraction for 2 hours, filtering to obtain a filter residue and a second filtrate, and then mixing the first filtrate and the second filtrate to obtain a mixed filtrate; S4. The mixed filtrate is concentrated under reduced pressure to 1.5 times the weight of the raw material, and then quickly put into a freeze dryer at a temperature of -40°C for freeze drying. Concentration and freezing need to be performed continuously to obtain self-assembled nanoparticles of rattan tea.

[0053] Detection and Analysis 1) Extract storage test The mixed filtrate obtained by extracting S3 in Example 1, the mixed filtrate obtained by extracting S3 in Control Example 1, and the mixed filtrate obtained by extracting S3 in Control Example 2 were stored at 4°C for 24 hours. The comparison results before and after were as follows: Figures 1 to 3 shown.

[0054] Figures 1 to 3 In the figure, from left to right are the extract of rattan tea alone (corresponding to control example 2), the extract of Panax notoginseng flower alone (corresponding to control example 1) and the mixed extract of Panax notoginseng flower and rattan tea (corresponding to example 1).

[0055] from Figures 1 to 3 From the comparative analysis, it can be seen that after the mixed extract of Panax notoginseng flower and rattan tea was stored at a low temperature of 4°C, the precipitate was very small, less than 1% of that when extracted separately. This proves that the saponin components in Panax notoginseng flower and dihydromyricetin in rattan tea have a natural self-assembly effect, thereby improving the stability of the rattan tea extract (flavonoids).

[0056] 2) Ingredient testing High performance liquid chromatography was used to determine dihydromyricetin and ginsenoside Rb3 in the mixed filtrate obtained by extracting the Panax notoginseng flower with a water content of not more than 10% and a total saponin content of not less than 15% and the rattan tea with a mildew degree of more than 40%, a water content of not more than 13% and a dihydromyricetin content of not less than 20% in Example 1 and Example 2, and the rattan tea with a mildew degree of more than 40%, a water content of not more than 13% and a dihydromyricetin content of not less than 20% in Control Example 2 at a mass ratio of 1:1, 2:1, 3:1, 4:1, 5:1, 6:1 and 0:1, respectively. The results are as follows Figure 4 and Figure 5 shown.

[0057] from Figure 4 The analysis showed that when the mass ratio of Panax notoginseng flower to rattan tea was 2:1 and 3:1, the content of dihydromyricetin was the highest. As the amount of Panax notoginseng flower increased, the content of dihydromyricetin showed a downward trend.

[0058] from Figure 5 The analysis showed that the content of saponin Rb3 increased with the increase of Panax notoginseng flower content, but when the mass ratio of Panax notoginseng flower to rattan tea was 4:1, the content of saponin Rb3 decreased significantly.

[0059] 1) Laser confocal microscopy test The SAN preparation prepared in Example 1 was tested by laser confocal microscopy. The results are as follows: Figure 6 shown.

[0060] from Figure 6 From the analysis, we can see that Figure 4 The laser confocal microscopy test results showed that the Panax notoginseng flower SAN preparation formed a uniform nanoparticle structure, indicating that the self-assembly process was successful.

[0061] 2) Yield analysis The yields of the Panax notoginseng flower SAN preparations prepared in Examples 1 to 4, the Panax notoginseng flower self-assembled nanoparticles prepared in Control Example 1, and the Ampelopsis ulmoides self-assembled nanoparticles prepared in Control Example 2 were calculated respectively.

[0062] The yield is calculated by the ratio of the total weight of the extract to the weight of the raw material. The specific formula is: Yield = (extract weight / raw material weight) × 100%. The result is as follows Figures 8 to 10 shown.

[0063] from Figures 8 to 10 Comprehensive analysis shows that when the ratio of Panax notoginseng flower to vine tea is between 1:1 and 6:1, the yield is high and stable. When it deviates from this ratio, the yield drops significantly, indicating that a specific ratio is crucial for the formation of self-assembled particles.

[0064] 3) Determination of ingredients in Panax notoginseng flower SAN wine The Sanqi flower SAN wine prepared in Example 5 was stored for 30 days and its component contents were detected. The alcohol content was measured by distillation, the total acid was measured by acid-base titration, the total sugar was measured by direct titration or colorimetry, the total ester was measured by gas chromatography, the dry extract was measured by loss on drying, cyanide was measured by isonicotinic acid-barbituric acid spectrophotometry, lead was measured by graphite furnace atomic absorption spectrometry, and sodium cyclamate was measured by high performance liquid chromatography. The test results are shown in Table 1.

[0065] Table 1 is the composition test results of Panax notoginseng flower SAN wine From the analysis in Table 1, we can see that the alcohol content (20℃): the test value is 42.3%vol, which is slightly higher than the standard requirement of 42%vol, but still within the qualified range, indicating that the alcohol content of the product meets the requirements and is well controlled.

[0066] Total acid content: The test result is 1.50g / L, which is significantly different from the standard requirement (≈6g / L), but is still considered qualified, indicating that the standard allows fluctuations within a certain range or that this indicator has specific requirements.

[0067] Total sugar content: The detected value is 0.6g / L, which is far below the standard requirement (≈2g / L), but still qualified, which may indicate that this is a low-sugar product.

[0068] Total ester content: The test result was 1.13g / L, which was significantly higher than the standard requirement (≈0.35g / L), but was judged to be qualified, indicating that the product performed well in terms of ester content.

[0069] Dry extract: The test value is 9.70g / L, which is much higher than the standard requirement (≈0.30g / L), indicating that the product has a high dry matter content.

[0070] Methanol content: The test result is <0.0591g / L, which is far lower than the standard requirement (≈0.6g / L), indicating that the product is highly safe.

[0071] Cyanide content: test result <1.0mg / L, in line with standard requirements (≈8.0mg / L).

[0072] Lead content: The test result is <0.04mg / kg, which meets the standard requirements (≈0.2mg / kg), indicating that heavy metal pollution is well controlled.

[0073] Saccharin: Not detected (quantitative limit 0.039g / kg), in line with the standard requirements (≈0.65g / kg), indicating that the product contains no or very small amounts of saccharin.

[0074] The above test results demonstrate that the Panax Notoginseng Flower SAN Liquor produced in accordance with the present invention meets national standards in all quality indicators, particularly with respect to safety indicators (such as methanol, cyanide, and lead), which are significantly below national standard limits, demonstrating its high safety. Some indicators, such as alcohol content, total acidity, and total sugar content, differ slightly from standard requirements but remain within acceptable ranges, demonstrating that the product possesses unique process characteristics or flavor profiles.

[0075] In summary, the method for preparing the self-assembled nanoparticles of Panax notoginseng flower and rattan tea of the present invention, by cleverly combining Panax notoginseng flower rich in saponins and rattan tea rich in dihydromyricetin as raw materials, through crushing, enzymatic hydrolysis, high temperature and high pressure extraction, concentration and freeze drying, successfully prepared the self-assembled nanoparticles of Panax notoginseng flower and rattan tea, and the preparation method has the advantages of simple operation, high extraction efficiency, green environmental protection, strong controllability, low cost and good product stability. The obtained self-assembled nanoparticles of Panax notoginseng flower and rattan tea not only retain the unique efficacy of the raw materials, but also show more superior bioavailability and stability due to the nanoscale effect, providing a solid foundation for the subsequent development of medicines, health products and functional foods, and have significant industrial application potential in the field of Panax notoginseng flower application technology.

[0076] The present invention utilizes self-assembled nanoparticles of Panax notoginseng flowers and rattan tea in wine. By innovatively applying these nanoparticles to blended liquor, the efficacy of Panax notoginseng flower saponins and rattan tea dihydromyricetin is fully utilized, while also providing an excellent taste. The resulting Panax notoginseng flower SAN liquor combines the aroma of volatile oils from Panax notoginseng flowers and rattan tea, the slightly bitter taste of saponins, and the aftertaste of dihydromyricetin, as well as the liver-protecting effects of Panax notoginseng flower saponins and dihydromyricetin. This allows consumers to enjoy not only a delicious taste but also the health benefits of Panax notoginseng flowers and rattan tea, fully satisfying the modern consumer's pursuit of a healthy lifestyle.

[0077] The above embodiments are only preferred embodiments for fully illustrating the present invention, and the protection scope of the present invention is not limited thereto. Any equivalent substitution or modification made by those skilled in the art based on the present invention is within the protection scope of the present invention.

Claims

1. A method for preparing self-assembled nanoparticles of Panax notoginseng flower and rattan tea, characterized in that: The following steps are involved: S1. Mix and grind the notoginseng flower and rattan tea to obtain a mixed powder; S2. Soak the mixed powder in hot water, then add a hydrolase to hydrolyze it, and filter to obtain an enzymatic filtrate; S3, adding water to the enzymatic hydrolysis filtrate, heating and pressurizing the water to obtain an extract; S4. The extract is concentrated under reduced pressure and freeze-dried to obtain self-assembled nanoparticles of Panax notoginseng flower and Ampelopsis pilosula.

2. The method for preparing the self-assembled nanoparticles of Panax notoginseng flower and Ampelopsis ulmoides according to claim 1, characterized in that: The mass ratio of the Panax notoginseng flower and the vine tea is 1:6 to 6:1; And / or, the volume ratio of the enzymatic hydrolysis filtrate to water is 1:15 to 1:

20.

3. The method for preparing the self-assembled nanoparticles of Panax notoginseng flower and Ampelopsis ulmoides according to claim 1, characterized in that: The hydrolase is selected from at least one of cellulase, pectinase and α-amylase.

4. The method for preparing the self-assembled nanoparticles of Panax notoginseng flower and Ampelopsis ulmoides according to claim 1, characterized in that: The temperature of the hot water for soaking the mixed powder is 50-65°C; And / or, the temperature of the heated pressurized water extraction is 90-100° C. and the pressure is at least 0.15 MPa; And / or, the time of the heated pressurized water extraction is 2 to 2.5 hours; And / or, the pressure of the reduced pressure concentration is 60KPa; and / or, the extract is concentrated under reduced pressure to 1.5 times the weight of the mixed powder; And / or, the freeze-drying temperature is -40°C.

5. The method for preparing the self-assembled nanoparticles of Panax notoginseng flower and Ampelopsis ulmoides according to claim 1, characterized in that: The Panax notoginseng flower is selected from the flower buds of Panax notoginseng that are at least three years old, the water content of the Panax notoginseng flower is not higher than 13%, and the total saponin content of the Panax notoginseng flower is not lower than 15%; And / or, the water content of the rattan tea is not higher than 13%, the degree of mildew is above 40%, and the content of dihydromyricetin is not lower than 20%.

6. A self-assembled nanoparticle of Panax notoginseng flower and rattan tea, characterized in that: The method is as described in any one of claims 1 to 5.

7. An application of self-assembled nanoparticles of Panax notoginseng flower and Ampelopsis ulmoides prepared by the preparation method according to any one of claims 1 to 5, characterized in that: The notoginseng flower and rattan tea self-assembled nanoparticles are used in wine.

8. The use according to claim 7, characterized in that The notoginseng flower and vine tea self-assembled nanoparticles are added into wine to obtain notoginseng flower SAN wine.

9. The use according to claim 8, characterized in that The preparation method of the Panax notoginseng flower SAN wine comprises the following steps: Add lotus leaves to water, boil for 1 hour, and add the self-assembled nanoparticles of Panax notoginseng flower and Ampelopsis ulmoides after cooling to obtain a desensitizing solution; The base wine, wine materials and alcohol-lowering liquid are mixed, and then placed at -40°C for 15 days, then returned to the temperature of 10°C, filtered, and the Panax notoginseng flower SAN wine is obtained.

10. The use according to claim 9, characterized in that The base liquor includes wheat wine with an alcohol content of not less than 50° and sauce-flavor liquor with an alcohol content of not less than 53°; And / or, the wine material includes an edible wine softener and edible glycerin, the edible wine softener is added in an amount of 0.5%, and the edible glycerin is added in an amount of 1.0%; and / or, after cooling to below 40° C., adding the notoginseng flower and vine tea self-assembled nanoparticles; And / or, the filtration includes three rounds of filtration, the first round of filtration is centrifugal filtration at a speed of not less than 4000 r / min, the second round of filtration is diatomaceous earth filtration, and the third round of filtration is membrane cross-flow filtration at a flow rate of 1 t / h.

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