Pharmaceutical composition for animal parasite expelling and preparation method and application thereof

Abstract

The invention relates to the technical field of pharmaceutical compositions, and particularly discloses a pharmaceutical composition for animal parasite expelling and a preparation method and application thereof. The pharmaceutical composition comprises the following raw material components in parts by mass: 15-30 parts of a traditional Chinese medicine extracting solution, 5-10 parts of doxycycline hydrochloride, 1-5 parts of vitamin C and 1-5 parts of mannan oligosaccharide, wherein the traditional Chinese medicine extracting solution comprises the following raw material components: a dark plum extracting solution, a pepper extracting solution, a wild buckwheat extracting solution, a gemma agrimoninae extracting solution, a rhizoma smilacis glabrae extracting solution and a cortex phellodendri extracting solution. The pharmaceutical composition for animal parasite expelling provided by the invention not only has an excellent parasite expelling effect, but also has the advantages of safe raw material components, no toxic or side effect on animal bodies, and difficult generation of drug resistance. According to the technical scheme, the problems that in the prior art, drugs for preventing and treating animal parasitic diseases are large in toxic and side effects, prone to generating drug resistance, poor in parasite expelling effect and narrow in prevention and treatment range are effectively solved.

Description

Technical Field

[0001] The present invention relates to the technical field of pharmaceutical compositions, and in particular to a pharmaceutical composition for animal deworming, a preparation method thereof, and an application thereof. Background Art

[0002] Parasitic diseases are common diseases in animals. These parasites mainly include roundworms, tapeworms, nematodes, hookworms, coccidia, flukes, and Giardia lamblia. These parasites mainly cause animals to have decreased appetite, malnutrition, poor spirits, diarrhea, and even bloody stools. Hookworms and flukes can also attach to the intestinal wall of animals and suck blood, causing anemia or even death. Parasitic infections can also lead to secondary intestinal obstruction or intussusception and other diseases, seriously affecting animal health. Moreover, with the increasing number of people keeping pets, parasitic diseases also pose a certain threat to human health. Therefore, the effective prevention and control of animal parasitic diseases is of great significance to improving animal health and maintaining human health.

[0003] Commonly used anthelmintics include macrolides such as selamectin, ivermectin, milbemycin, and moxidectin; neonicotinoids such as imidacloprid; and broad-spectrum insecticides such as praziquantel and fepronil. However, these Western medicines all have certain toxic side effects, and prolonged use can cause animals to develop drug resistance, significantly reducing their anthelmintic effectiveness. They can also place a certain burden on the animal's internal organs, reduce their immunity, and harm their health. There are also reports of a traditional Chinese medicine composition for animal anthelmintics that uses allicin in combination with other traditional Chinese medicine components to prevent and treat toxoplasmosis in animals, but only for nematodes. There are also reports of anthelmintics made from traditional Chinese medicine components such as chinaberry bark extract, arecoline, and toosendanin, but their effectiveness in preventing and treating animal surface parasites is poor.

[0004] Based on this, the development of a drug with excellent anthelmintic effect, small toxic side effects and the ability to prevent and treat a variety of parasites has important practical significance for the prevention and treatment of animal diseases. Summary of the Invention

[0005] To address the problems of existing drugs for preventing and treating animal parasitic diseases, such as significant toxic side effects, the development of drug resistance, poor anthelmintic efficacy, and a limited control range, the present invention provides a pharmaceutical composition for animal anthelmintics, as well as its preparation method and application. The raw materials of the pharmaceutical composition for animal anthelmintics include a traditional Chinese medicine extract, doxycycline hydrochloride, vitamin C, and mannooligosaccharides. By utilizing the synergistic effect of these raw materials, the pharmaceutical composition for animal anthelmintics has excellent anthelmintic efficacy, low toxic side effects, and is not prone to drug resistance, thus overcoming the shortcomings of the existing technology.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions: A first aspect of the present invention provides a pharmaceutical composition for animal deworming, comprising the following raw material components in parts by weight: 15-30 parts of a traditional Chinese medicine extract, 5-10 parts of doxycycline hydrochloride, 1-5 parts of vitamin C, and 1-5 parts of manno-oligosaccharides; wherein the raw material components of the traditional Chinese medicine extract include a black plum extract, a Zanthoxylum bungeanum extract, a golden buckwheat extract, a herbaceous agrimony extract, a smilax glabra extract, and a phellodendron amurense extract.

[0007] Compared with the prior art, the present invention designs a pharmaceutical composition for animal deworming, which uses a traditional Chinese medicine extract as the main deworming ingredient. Among them, the ingredients such as ebony acid and neem acid contained in the black plum extract can paralyze parasites; at the same time, the black plum extract can change the local acid-base environment of the intestine, making it difficult for parasites to adapt, thereby inhibiting the growth and reproduction of parasites. The isolimonene and linalool in the Zanthoxylum bungeanum extract can act on the parasite's nervous system, affecting the conduction of the parasite's nerve signals, thereby seriously affecting the parasite's perception and motor functions, achieving the purpose of deworming; and the flavonoids in the Zanthoxylum bungeanum extract can interfere with the hormonal balance in the parasite's body, disrupting its development process, leading to the death of the parasite or loss of reproductive ability. The total phenol and flavonoid content in the golden buckwheat extract is relatively high, giving the pharmaceutical composition a strong antioxidant capacity. The antioxidant effect of golden buckwheat can affect the parasite's oxidation-antioxidation balance, reduce its survival ability, and achieve the purpose of efficient deworming. Agrimonial in agrimony extract can significantly affect the parasite's neuromuscular system, causing tonic contractions of the parasite's muscles, leading to spasms and paralysis. Especially effective in expelling tapeworms, agrimononial can quickly penetrate the tapeworm's body wall, causing spasms and death, achieving the desired deworming effect. The polysaccharide components in Smilax glabra extract have immunomodulatory effects, activating immune cells in animals, such as macrophages. This not only boosts the animal's immunity but also makes parasites easier to eliminate. The active ingredients in Phellodendron chinense extract can disrupt the integrity of parasite cell membranes, increasing their permeability and affecting their normal metabolic processes, thereby inhibiting their growth and reproduction.

[0008] The present invention also adds doxycycline hydrochloride to the pharmaceutical composition. Since parasitic infections are accompanied by bacterial infections, and some parasites themselves have metabolic patterns that are highly similar to those of bacteria, such as Toxoplasma and ticks, the addition of doxycycline hydrochloride can not only effectively inhibit the growth and reproduction of bacteria, but also directly affect the protein synthesis process in the parasites, interfering with their normal growth and development. The addition of mannooligosaccharides can regulate the intestinal microbial flora of animals and increase the animal body's resistance to parasitic infections. The structure of mannooligosaccharides makes it easier for parasites to attach and be excreted from the body with feces, reducing the number of parasites in the intestines and achieving the purpose of deworming. Vitamin C can help increase the level of antibodies in animals, enabling the animal body to better resist the invasion of pathogens and parasites.

[0009] Preferably, the Chinese medicine extract comprises the following raw material components in percentage by mass: 10%-35% of black plum extract, 10%-20% of Zanthoxylum bungeanum extract, 10%-25% of golden buckwheat extract, 10%-30% of cranesbill extract, 10%-20% of wild yam extract and 10%-20% of phellodendron amurense extract.

[0010] Preferably, the preparation method of the black plum extract comprises the following steps: crushing dried black plums, dispersing the dried plums in the extract, extracting at 40° C.-60° C. for 3 h-5 h, and separating the solid and liquid to obtain the black plum extract.

[0011] Black plum extract can also promote the secretion of immune factors in animals. These immune factors can regulate the function of immune cells, enhance the intensity and persistence of immune responses, and help reduce the number of parasites in animals.

[0012] Preferably, the method for preparing the Zanthoxylum bungeanum extract comprises the following steps: soaking the Zanthoxylum bungeanum in deionized water, allowing the water to stand, boiling the water, and separating the solid from the liquid to obtain the Zanthoxylum bungeanum extract.

[0013] The active ingredients of Sichuan pepper extract can also promote the phagocytic function of macrophages in animal bodies, enabling them to more actively identify and attack parasites.

[0014] Further preferably, the extract is an ethanol solution with a mass concentration of 40%-55%.

[0015] Further preferably, the mass volume ratio of the dried plums to the extract is 1g:5mL-1g:10mL.

[0016] Further preferably, the mass volume ratio of the Zanthoxylum bungeanum and deionized water is 1g:10mL-1g:15mL.

[0017] More preferably, the standing time is 2h-3h.

[0018] Further preferably, the specific operation of solid-liquid separation in the Zanthoxylum bungeanum extract comprises the following steps: repeatedly boiling and filtering the solid filtrate 2-4 times, combining the filtrate, and concentrating to obtain the Zanthoxylum bungeanum extract.

[0019] More preferably, the boiling time is 50 min-80 min.

[0020] More preferably, the concentration temperature is 110° C.-120° C., and the concentration time is 40 min-60 min.

[0021] Preferably, the preparation method of the golden buckwheat extract comprises the following steps: dispersing the golden buckwheat in an alcohol solution, performing ultrasonic treatment at 40°C-50°C, and performing solid-liquid separation to obtain the golden buckwheat extract.

[0022] Preferably, the preparation method of the agrimony extract comprises the following steps: crushing the agrimony, dispersing it in an alcohol solution, performing ultrasonic treatment at 45° C.-65° C., and performing solid-liquid separation to obtain the agrimony extract.

[0023] Further preferably, the mass volume ratio of the golden buckwheat to the alcohol solution is 1g:3mL-1g:5mL.

[0024] Furthermore, the mass volume ratio of the agrimony bud grass to the alcohol solution is 1g:4mL-1g:6mL.

[0025] Furthermore, the alcohol solution is an ethanol solution with a mass concentration of 35%-50%.

[0026] Furthermore, the frequency of the ultrasonic treatment is 30kW-40kW, and the time of the ultrasonic treatment is 20min-40min.

[0027] Preferably, the method for preparing the Smilax glabra extract comprises the following steps: soaking the Smilax glabra in deionized water, performing ultrasonic treatment at 60-70° C., and performing solid-liquid separation to obtain the Smilax glabra extract.

[0028] Preferably, the preparation method of the Phellodendron chinense extract comprises the following steps: crushing the Phellodendron chinense and soaking it in organic alcohol, boiling it, keeping it boiling for 30-40 minutes, separating the solid and the liquid, and concentrating it to obtain the Phellodendron chinense extract.

[0029] Further preferably, the mass volume ratio of the Smilax glabra to deionized water is 1g:4mL-1g:6mL.

[0030] Further preferably, the frequency of the ultrasonic treatment is 35kW-40kW, and the time of the ultrasonic treatment is 30min-45min.

[0031] Further preferably, the mass volume ratio of the Phellodendron chinense to the organic alcohol is 1g:10mL-1g:15mL.

[0032] More preferably, the organic alcohol is anhydrous ethanol.

[0033] More preferably, the concentration temperature is 80° C.-90° C., and the concentration time is 10 min-30 min.

[0034] The second aspect of the present invention provides a method for preparing the pharmaceutical composition for animal deworming, comprising the following steps: weighing the raw materials of each component according to the designed ratio, mixing them uniformly, and obtaining the pharmaceutical composition for animal deworming.

[0035] The third aspect of the present invention provides the use of the pharmaceutical composition for animal deworming or the pharmaceutical composition for animal deworming prepared by the method for preparing the pharmaceutical composition for animal deworming in the preparation of animal deworming pharmaceutical preparations.

[0036] In summary, the pharmaceutical composition for animal deworming provided by the present invention not only has excellent deworming effects, but also has safe raw material ingredients, is non-toxic to the animal body, and is not prone to drug resistance. The technical solution of the present invention effectively solves the problems of existing drugs for preventing and treating animal parasitic diseases, such as severe toxic side effects, the susceptibility to drug resistance, poor deworming effects, and a narrow control range. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 The figures show the therapeutic effect of transdermal drops prepared by using the pharmaceutical composition for animal deworming obtained in various examples and comparative examples on intestinal nematode disease in piglets; Figure 2 The figures show the therapeutic effect of the transdermal drops prepared by using the pharmaceutical composition for animal deworming obtained in each embodiment and comparative example on the expelling of fleas from cats. DETAILED DESCRIPTION

[0038] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0039] The preparation method of the black plum extract used in the following examples and comparative examples includes the following steps: crushing 200g of dried black plums, dispersing them in 1600mL of 45% ethanol solution, extracting at 50°C for 4h, and separating the solid and liquid to obtain the black plum extract.

[0040] The preparation method of the Zanthoxylum bungeanum extract used in the following examples and comparative examples comprises the following steps: soaking 200 g of Zanthoxylum bungeanum in 2400 mL of deionized water, letting it stand for 3 h, boiling it for 60 min, separating the solid and the liquid, repeating the above steps three times for the solid filtrate, combining the filtrate, and concentrating it at 115° C. for 50 min to obtain the Zanthoxylum bungeanum extract.

[0041] The preparation method of the golden buckwheat extract used in the following examples and comparative examples includes the following steps: dispersing 200 g of golden buckwheat in 800 mL of 40% ethanol solution, ultrasonically treating at 45° C. and 35 kW for 30 min, and performing solid-liquid separation to obtain the golden buckwheat extract.

[0042] The preparation method of the Herba Lycopodii extract used in the following examples and comparative examples comprises the following steps: crushing 200 g of Herba Lycopodii, dispersing it in 1000 mL of 45% ethanol solution, ultrasonically treating it at 60° C. and 38 kW for 30 min, and separating the solid and liquid to obtain the Herba Lycopodii extract.

[0043] The preparation method of the Smilax glabra extract used in the following examples and comparative examples comprises the following steps: soaking 200 g of Smilax glabra in 1000 mL of deionized water, performing ultrasonic treatment at 65° C. and 40 kW for 40 min, and performing solid-liquid separation to obtain the Smilax glabra extract.

[0044] The preparation method of the Phellodendron chinense extract used in the following examples and comparative examples includes the following steps: 200g of Phellodendron chinense is crushed and soaked in 2500mL of anhydrous ethanol, boiled, kept boiling for 35min, solid-liquid separation, and concentrated at 85°C for 20min to obtain the Phellodendron chinense extract.

[0045] Example 1 This embodiment provides a pharmaceutical composition for animal deworming and a preparation method thereof, which specifically includes the following contents: The pharmaceutical composition for animal deworming comprises the following raw material components in parts by weight: 20 parts of traditional Chinese medicine extract, 8 parts of doxycycline hydrochloride, 3 parts of vitamin C and 3 parts of mannooligosaccharide; wherein the traditional Chinese medicine extract comprises the following raw material components in percentage by weight: 25% of black plum extract, 15% of Zanthoxylum bungeanum extract, 15% of golden buckwheat extract, 15% of cranesbill extract, 20% of smilax glabra extract and 10% of phellodendron amurense extract.

[0046] The preparation method of the pharmaceutical composition for animal deworming comprises the following steps: weighing raw materials of various components according to a designed ratio, and mixing them uniformly to obtain the pharmaceutical composition for animal deworming.

[0047] Example 2 This embodiment provides a pharmaceutical composition for animal deworming and a preparation method thereof, which specifically includes the following contents: The pharmaceutical composition for animal deworming comprises the following raw material components in parts by weight: 25 parts of traditional Chinese medicine extract, 6 parts of doxycycline hydrochloride, 4 parts of vitamin C and 4 parts of mannooligosaccharide; wherein the traditional Chinese medicine extract comprises the following raw material components in percentage by weight: 18% of black plum extract, 18% of Zanthoxylum bungeanum extract, 22% of golden buckwheat extract, 20% of cranesbill extract, 10% of smilax glabra extract and 10% of phellodendron amurense extract.

[0048] The preparation method of the pharmaceutical composition for animal deworming comprises the following steps: weighing raw materials of various components according to a designed ratio, and mixing them uniformly to obtain the pharmaceutical composition for animal deworming.

[0049] Example 3 This embodiment provides a pharmaceutical composition for animal deworming and a preparation method thereof, which specifically includes the following contents: The pharmaceutical composition for animal deworming comprises the following raw material components in parts by weight: 27 parts of traditional Chinese medicine extract, 5 parts of doxycycline hydrochloride, 2 parts of vitamin C and 5 parts of mannooligosaccharide; wherein the traditional Chinese medicine extract comprises the following raw material components in percentage by weight: 14% of black plum extract, 12% of Zanthoxylum bungeanum extract, 20% of golden buckwheat extract, 20% of cranesbill extract, 15% of smilax glabra extract and 19% of phellodendron amurense extract.

[0050] The preparation method of the pharmaceutical composition for animal deworming comprises the following steps: weighing raw materials of various components according to a designed ratio, and mixing them uniformly to obtain the pharmaceutical composition for animal deworming.

[0051] Comparative Example 1 This comparative example provides a pharmaceutical composition for animal deworming and a preparation method thereof, which differs from Example 1 in that: the doxycycline hydrochloride is replaced by an equal amount of metacycline, and the other ingredients and contents remain unchanged, specifically including the following contents: The pharmaceutical composition for animal deworming comprises the following raw materials in parts by weight: 20 parts of a traditional Chinese medicine extract, 8 parts of metacycline, 3 parts of vitamin C, and 3 parts of mannooligosaccharide; wherein the traditional Chinese medicine extract comprises the following raw materials in percentage by weight: 25% of a black plum extract, 15% of a Zanthoxylum bungeanum extract, 15% of a golden buckwheat extract, 15% of agrimony bud grass extract, 20% of a smilax glabra extract, and 10% of a phellodendron amurense extract.

[0052] The preparation method of the pharmaceutical composition for animal deworming comprises the following steps: weighing raw materials of various components according to a designed ratio, and mixing them uniformly to obtain the pharmaceutical composition for animal deworming.

[0053] Comparative Example 2 This comparative example provides a pharmaceutical composition for animal deworming and a preparation method thereof, which differs from Example 1 in that the black plum extract in the traditional Chinese medicine extract is replaced with the sophora flavescens extract, while the other ingredients and contents remain unchanged, specifically including the following: The pharmaceutical composition for animal deworming comprises the following raw material components in parts by weight: 20 parts of a traditional Chinese medicine extract, 8 parts of doxycycline hydrochloride, 3 parts of vitamin C, and 3 parts of mannooligosaccharide; wherein the traditional Chinese medicine extract comprises the following raw material components in percentage by weight: 25% of a sophora flavescens extract, 15% of a zanthoxylum bungeanum extract, 15% of a golden buckwheat extract, 15% of agrimony bud herb extract, 20% of a smilax glabra extract, and 10% of a phellodendron amurense extract.

[0054] The preparation method of the Sophora flavescens extract comprises the following steps: crushing 200 g of Sophora flavescens, dispersing the mixture in 1600 mL of 45% ethanol solution, extracting the mixture at 50° C. for 4 h, and performing solid-liquid separation to obtain the Sophora flavescens extract.

[0055] The preparation method of the pharmaceutical composition for animal deworming comprises the following steps: weighing raw materials of various components according to a designed ratio, and mixing them uniformly to obtain the pharmaceutical composition for animal deworming.

[0056] Comparative Example 3 This comparative example provides a pharmaceutical composition for animal deworming and a preparation method thereof, which differs from Example 1 in that the golden buckwheat extract in the traditional Chinese medicine extract is replaced with an wormwood extract, and the other ingredients and contents remain unchanged, specifically including the following: The pharmaceutical composition for animal deworming comprises the following raw material components in parts by weight: 20 parts of a traditional Chinese medicine extract, 8 parts of doxycycline hydrochloride, 3 parts of vitamin C, and 3 parts of mannooligosaccharide; wherein the traditional Chinese medicine extract comprises the following raw material components in percentage by weight: 25% of a sophora flavescens extract, 15% of a zanthoxylum bungeanum extract, 15% of an artemisia argyi extract, 15% of agrimony bud herb extract, 20% of a smilax glabra extract, and 10% of a phellodendron amurense extract.

[0057] The preparation method of the mugwort extract includes the following steps: soaking 200g of mugwort in 2400mL of deionized water, letting it stand for 3h, boiling it for 60min, separating the solid and liquid, repeating the above steps 3 times on the solid filter, combining the filtrate, and concentrating it at 115°C for 50min to obtain the mugwort extract.

[0058] The preparation method of the pharmaceutical composition for animal deworming comprises the following steps: weighing raw materials of various components according to a designed ratio, and mixing them uniformly to obtain the pharmaceutical composition for animal deworming.

[0059] Comparative Example 4 This comparative example provides a pharmaceutical composition for animal deworming and a preparation method thereof, which differs from Example 1 in that the component mannooligosaccharide is omitted, while the other components and contents remain unchanged, specifically including the following contents: The pharmaceutical composition for animal deworming comprises the following raw material components in parts by weight: 20 parts of a traditional Chinese medicine extract, 8 parts of doxycycline hydrochloride, and 3 parts of vitamin C; wherein the traditional Chinese medicine extract comprises the following raw material components in percentage by weight: 25% of a black plum extract, 15% of a Zanthoxylum bungeanum extract, 15% of a golden buckwheat extract, 15% of agrimony bud grass extract, 20% of a smilax glabra extract, and 10% of a phellodendron amurense extract.

[0060] The preparation method of the pharmaceutical composition for animal deworming comprises the following steps: weighing raw materials of various components according to a designed ratio, and mixing them uniformly to obtain the pharmaceutical composition for animal deworming.

[0061] In order to further confirm the technical effect of the present invention, the present invention conducted the following tests on the pharmaceutical compositions for animal deworming obtained in Examples 1-3 and Comparative Examples 1-4: each pharmaceutical composition for animal deworming was prepared into deworming transdermal drops, and the preparation method was as described in the test example.

[0062] Test example 5 g of the pharmaceutical composition for animal deworming obtained in each of the examples and comparative examples was dissolved in 20 g of a 50 wt% ethanol solution, and 20 g of laurocapram, 50 g of propylene glycol, 2 g of butylated hydroxyanisole, 30 g of soybean oil and 5 g of polyglycerol ricinoleate were added and mixed uniformly to obtain transdermal drops for animal deworming.

[0063] Performance test 1: 6-8 week old piglets diagnosed with intestinal nematode disease were used as experimental animals, half male and half female, and randomly divided into nine groups, with 20 piglets in each group. The blank group was given normal saline, and the Western medicine group was given commercially available ivermectin transdermal drops. The anthelmintic transdermal drops prepared using the pharmaceutical composition for animal anthelmintic obtained in Examples 1-3 and Comparative Examples 1-4 were applied to piglets in different groups. The application method is as follows: Pour the transdermal drops from the shoulder backward along the midline of the piglet's back. The dosage of the transdermal drops is 0.03 mL / kg per piglet, three times daily for three consecutive days. Starting on the fourth day, observe the piglets' feces. Three consecutive fecal examinations showing negative results for parasite eggs are considered cured. The cure rate (%) = number of piglets cured / number of piglets affected. The anthelmintic efficacy of the transdermal drops is evaluated according to the following criteria: a cure rate above 95% is considered excellent, a cure rate between 60% and 90% is considered good, and a cure rate below 60% is considered poor. After administration, observe each piglet's skin for noticeable reddening to assess skin irritation. A reddening of 0-1 piglets is considered mild, a reddening of 2-4 piglets is considered good, and a reddening of 5 or more piglets is considered irritating. The test results are shown in Table 1.

[0064] Table 1 Test results 1

[0065] Performance test 2: Pet cats aged 12-24 months, half male and half female, were randomly divided into 9 groups of 10 each. The pet cats were cleaned and their body surfaces were cleaned. Then, about 100 cat fleas were infested on the body surface of each cat. Two days after the infestation, each group of cats was treated. The blank group was given normal saline, and the Western medicine group was given commercially available pyroxil transdermal drops. The anthelmintic transdermal drops prepared using the pharmaceutical composition for animal anthelmintic obtained in Examples 1-3 and Comparative Examples 1-4 were used on the remaining different groups of cats. The method of use was as follows: from the shoulder to the back, along the midline of the back, the transdermal drops were poured at a dose of 0.03 mL / kg for each cat, twice a day, for three consecutive days. Starting on the fourth day, the number of cat fleas visible in the field of view of each cat was observed and counted, and the anthelmintic rate was calculated. The anthelmintic rate (%) = (number of cat fleas before anthelmintic - number of cat fleas after anthelmintic) / number of cat fleas before anthelmintic; the anthelmintic effect on body surface parasites was evaluated. The evaluation criteria were as follows: cats with 5-10 fleas for two consecutive days were rated as excellent; cats with 20-30 fleas for two consecutive days were rated as good; and cats with 30 or more fleas for two consecutive days were rated as poor. After administration, each cat was observed for noticeable reddening of the skin at the administration site to assess the irritation of the transdermal drops. Reddening of 0-2 fleas was rated as mild, 3-5 fleas as good, and 6 or more fleas as irritating. The test results are shown in Table 2.

[0066] Table 2 Test results 2

[0067] Performance Test 3. Cats aged 12-24 months diagnosed with toxoplasmosis were used as experimental animals, half male and half female, and randomly divided into nine groups, 20 in each group. The blank group was given normal saline, and the western medicine group was given a commercially available Bayer internal anthelmintic. The anthelmintic transdermal drops prepared using the pharmaceutical composition for animal anthelmintic obtained in Examples 1-3 and Comparative Examples 1-4 were used on cats in different groups. The method of use was as follows: from the shoulder of the cat backward, along the midline of the back, the dose of the transdermal drops for each cat was 0.03 mL / kg, three times a day, for three consecutive days. The cat feces were observed starting from the fourth day, and the anthelmintic rate was calculated based on the number of worm eggs in the feces discharged for three consecutive times, and the anthelmintic effect was evaluated. Among them, the deworming rate (%) = (the number of eggs before deworming - the number of eggs after deworming) / the number of eggs before deworming; the average value of each group was taken, and the evaluation criteria were as follows: the deworming rate above 90% was recorded as excellent, the deworming rate between 60% and 85% was recorded as good, and the deworming rate below 60% was recorded as poor. This was used to evaluate the deworming effect of transdermal drops on internal parasites. The test results are shown in Table 3.

[0068] Table 3 Test results 3

[0069] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions or improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A pharmaceutical composition for animal deworming, characterized in that: The pharmaceutical composition comprises the following raw material components in parts by weight: 15-30 parts of traditional Chinese medicine extract, 5-10 parts of doxycycline hydrochloride, 1-5 parts of vitamin C and 1-5 parts of mannooligosaccharide; wherein the traditional Chinese medicine extract comprises black plum extract, Zanthoxylum bungeanum extract, golden buckwheat extract, agrimony bud extract, smilax glabra extract and phellodendron amurense extract.

2. The pharmaceutical composition for animal anthelmintic treatment according to claim 1, wherein: The traditional Chinese medicine extract comprises the following components in percentage by mass: 10%-35% of black plum extract, 10%-20% of pepper extract, 10%-25% of golden buckwheat extract, 10%-30% of cranesbill extract, 10%-20% of smilax glabra extract and 10%-20% of phellodendron amurense extract.

3. The pharmaceutical composition for animal anthelmintic treatment according to claim 1 or 2, wherein: The preparation method of the black plum extract comprises the following steps: crushing dried black plums, dispersing the mixture in an alcohol solution, extracting the mixture at 40-60° C. for 3-5 hours, and performing solid-liquid separation to obtain the black plum extract; and / or The preparation method of the pepper extract comprises the following steps: soaking the pepper in deionized water, allowing the water to stand, boiling the water, and separating the solid from the liquid to obtain the pepper extract.

4. The pharmaceutical composition for animal anthelmintic treatment according to claim 3, wherein: The alcohol solution is an ethanol solution with a mass concentration of 40%-55%; and / or The mass volume ratio of the dried plums to the alcohol solution is 1g:5mL-1g:10mL; and / or The mass volume ratio of the Zanthoxylum bungeanum to deionized water is 1g:10mL-1g:15mL; and / or The standing time is 2h-3h; and / or The boiling time is 50 min-80 min.

5. The pharmaceutical composition for animal anthelmintic treatment according to claim 1 or 2, wherein: The preparation method of the golden buckwheat extract comprises the following steps: dispersing the golden buckwheat in an alcohol solution, performing ultrasonic treatment at 40° C.-50° C., and performing solid-liquid separation to obtain the golden buckwheat extract; and / or The preparation method of the agrimony extract comprises the following steps: crushing the agrimony, dispersing the agrimony in an alcohol solution, performing ultrasonic treatment at 45° C.-65° C., and performing solid-liquid separation to obtain the agrimony extract.

6. The pharmaceutical composition for animal anthelmintic treatment according to claim 5, wherein: The mass volume ratio of the golden buckwheat to the alcohol solution is 1g:3mL-1g:5mL; and / or The mass volume ratio of the agrimony bud grass to the alcohol solution is 1g:4mL-1g:6mL; and / or The alcohol solution is an ethanol solution with a mass concentration of 35%-50%; and / or The frequency of the ultrasonic treatment is 30kW-40kW, and the time of the ultrasonic treatment is 20min-40min.

7. The pharmaceutical composition for animal anthelmintic treatment according to claim 1 or 2, wherein: The preparation method of the Smilax glabra extract comprises the following steps: soaking the Smilax glabra in deionized water, performing ultrasonic treatment at 60-70° C., and performing solid-liquid separation to obtain the Smilax glabra extract; and / or The preparation method of the Phellodendron chinense extract comprises the following steps: crushing the Phellodendron chinense and soaking it in organic alcohol, boiling it, keeping it boiling for 30 minutes to 40 minutes, separating the solid and the liquid, and concentrating it to obtain the Phellodendron chinense extract.

8. The pharmaceutical composition for animal anthelmintic treatment according to claim 7, wherein: The mass volume ratio of the Smilax glabra to deionized water is 1g:4mL-1g:6mL; and / or The frequency of the ultrasonic treatment is 35kW-40kW, and the time of the ultrasonic treatment is 30min-45min; and / or The mass volume ratio of the cortex phellodendri to the organic alcohol is 1g:10mL-1g:15mL; and / or The organic alcohol is anhydrous ethanol; and / or The concentration temperature is 80° C.-90° C., and the concentration time is 10 min-30 min.

9. A method for preparing a pharmaceutical composition for animal anthelmintic treatment according to any one of claims 1 to 8, characterized in that: The method comprises the following steps: weighing raw materials of various components according to a designed ratio, mixing them evenly, and obtaining a medicinal composition for animal deworming.

10. Use of the pharmaceutical composition for animal anthelmintics according to any one of claims 1 to 8 or the pharmaceutical composition for animal anthelmintics prepared by the method for preparing the pharmaceutical composition for animal anthelmintics according to claim 9 in preparing an animal anthelmintic pharmaceutical preparation.

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