Organic fertilizer suitable for increasing yield and killing pests of tea leaves and preparation method of organic fertilizer

Abstract

The invention discloses an organic fertilizer suitable for tea yield increase and insect killing and a preparation method thereof, and relates to the technical field of organic fertilizer and microbial fertilizer manufacturing. The organic fertilizer is prepared from straw, chicken manure, radix inulae, clinacanthus nutans, affine cudweed, periploca forrestii schltr, ellagic acid-rice protein zymolyte, sodium laurate modified diatomite, sodium humate, urea, compound microorganism bacteria and water. The compound microorganism bacterium is composed of candida utilis saccharomycetes and neurospora intermedia. The compound microorganism bacteria in the organic fertilizer are beneficial to accelerating fermentation decomposition of the straw, the chicken manure, the sodium humate, the urea and the traditional Chinese medicine plants and promoting macromolecular substances to be converted into micromolecular active ingredients, then ellagic acid-rice protein zymolyte and sodium laurate modified diatomite are supplemented, and the yield of tea tree planting is increased through reasonable proportion of nutritional ingredients.

Description

Technical Field

[0001] The invention relates to the technical field of manufacturing organic fertilizers and microbial fertilizers, and in particular to an organic fertilizer suitable for increasing tea production and killing insects, and a preparation method thereof. Background Art

[0002] Currently, many types of fertilizers are used in tea cultivation. Most commercially available nitrogen, phosphorus, and potassium compound fertilizers, primarily composed of the three inorganic elements N, P, and K, can meet the growth needs of tea trees in the short term. However, long-term use can damage the soil aggregate structure, leading to soil compaction and hardening, and a lack of effective insecticide control, which in turn affects tea tree growth and reduces tea quality and yield. Therefore, there is an urgent need to develop an organic fertilizer suitable for increasing tea production and killing insects. Summary of the Invention

[0003] In view of the above-mentioned defects of the prior art, the present invention proposes an organic fertilizer suitable for increasing tea production and killing insects and a preparation method thereof, so as to solve the problems raised by the above-mentioned background technology.

[0004] In order to achieve the above object, the present invention provides the following technical solutions:

[0005] An organic fertilizer suitable for increasing tea production and killing insects comprises the following raw materials in parts by weight: 50-70 parts of straw, 30-40 parts of chicken manure, 15-25 parts of Tibetan woodruff (roots of Inula racemosa Hook.f. and Inula helenium L., plants of the Asteraceae family), 15-20 parts of Clinacanthus nutans (Burm.F.) Lindau, plant of the Acanthaceae family), 12-22 parts of Gnaphalium affine D.Don, plant of the Asteraceae family), and 10-18 parts of Periploca forrestii (roots of Periploca forrestii, plant of the Asclepiadaceae family). Schltr. root or whole plant), 8-15 parts of ellagic acid-rice protein hydrolysate, 5-10 parts of sodium laurate modified diatomaceous earth, 5-10 parts of sodium humate, 4-15 parts of urea, 1-6 parts of composite microorganisms and 200-300 parts of water;

[0006] The composite microorganisms are composed of Candida utilis and Neurospora intermedia in a mass ratio of (1-5): (2-7).

[0007] Preferably, an organic fertilizer suitable for increasing tea production and killing insects comprises the following raw materials in parts by weight: 60 parts of straw, 35 parts of chicken manure, 20 parts of Tibetan wood fragrance, 18 parts of hyssopus herba, 15 parts of kohlrabi herba, 12 parts of schisandra chinensis, 12 parts of ellagic acid-rice protein hydrolysate, 8 parts of sodium laurate-modified diatomaceous earth, 7 parts of sodium humate, 6 parts of urea, 5 parts of composite microorganisms, and 280 parts of water;

[0008] The composite microorganism consists of Candida utilis and Neurospora intermedia at a mass ratio of 2:5.

[0009] Preferably, the culture method of Candida utilis is to fully dissolve the purchased freeze-dried powder of Candida utilis with sterile water, spread it evenly on a plate, and culture it in a culture medium (yeast extract: 3g, malt extract: 3g, glucose: 10g, casein peptone: 5g, agar: 20g). The culture conditions are: pH: 6.2±0.2, temperature 25°C.

[0010] Preferably, the culture method of Neurospora intermedia is to fully dissolve the purchased freeze-dried powder of Neurospora intermedia with sterile water, spread it evenly on a plate, and place it in a culture medium (potato extract: 10g, glucose: 20g, KH2PO4: 3g, MgSO4·7H2O: 1.5g, thiamine: 0.008g, agar: 15g) for culture. The culture conditions are: pH: 6.0±0.2, temperature 25°C.

[0011] Preferably, the method for preparing the ellagic acid-rice protein hydrolysate comprises the following steps:

[0012] (1) Rice is crushed, and water is added and preheated to 40-50° C., the pH is adjusted to 9-11, and alkaline protease is added for enzymolysis for 200-300 min. After the enzymolysis is completed, the pH is adjusted to 8.5-9.6, and the enzyme is inactivated to obtain an enzymolysis solution;

[0013] (2) dissolving ellagic acid in anhydrous ethanol to obtain an ellagic acid solution;

[0014] (3) The enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2) are mixed and stirred for 60 to 120 minutes to obtain ellagic acid-rice protein hydrolysate.

[0015] Preferably, the alkaline protease has an enzymatic activity of 100,000 to 200,000 U / g, is a brown powder, has a CAS number of 9014-01-1, and is purchased from Chengdu Wanxiang Hongrun Biotechnology Co., Ltd.

[0016] Preferably, in step (1), the material-liquid ratio of rice to water is (20-30) g:100 mL.

[0017] Preferably, in step (2), the material-liquid ratio of ellagic acid to anhydrous ethanol is 1 g: (10-20) mL.

[0018] Preferably, the preparation method of the sodium laurate modified diatomaceous earth comprises the following steps:

[0019] (a) adding diatomaceous earth to a 10-15 wt% sodium hydroxide aqueous solution and soaking it for 15-40 minutes, and filtering to obtain pretreated diatomaceous earth;

[0020] (b) adding sodium laurate and water to the pretreated diatomaceous earth obtained in step (a), stirring for 10 to 15 minutes, cooling and drying, and then grinding to obtain sodium laurate-modified diatomaceous earth.

[0021] Preferably, in step (a), the material-liquid ratio of diatomaceous earth to sodium hydroxide aqueous solution is 1 g: (3-7) mL.

[0022] Preferably, in step (b), the material-liquid ratio of the pretreated diatomaceous earth, sodium laurate and water is 1 g: (1.5-3) mL: (20-50) mL.

[0023] Preferably, a method for preparing an organic fertilizer suitable for increasing tea production and killing insects comprises the following steps:

[0024] S1, weighing the raw materials according to the weight parts;

[0025] S2. First, crush the Tibetan wood fragrance, the herb of yutun, the herb of guqin and the vine of fujian, then add sodium humate and water, soak for 100 to 200 minutes, then add urea, mix well, and obtain the fermentation product;

[0026] S3, adding straw, chicken manure and composite microorganisms to the fermented material obtained in step S2, performing aerobic fermentation at a temperature of 25-28° C. for 10-20 days, and drying to a moisture content of less than 12-20 wt % to obtain a fermentation product;

[0027] S4. Add ellagic acid-rice protein hydrolysate and sodium laurate modified diatomaceous earth to the fermentation product obtained in step S3, mix well, and granulate to obtain organic fertilizer.

[0028] Preferably, a method for applying an organic fertilizer suitable for increasing tea production and killing insects is provided, wherein the organic fertilizer is applied in one or more of the following ways: hole application, furrow application, and broadcast application, with a dosage of 40 to 60 kg per mu.

[0029] Preferably, the organic fertilizer involved in this application is used to improve the yield and quality of spring tea and prevent and / or kill tea pests.

[0030] Preferably, the tea pest is the tea leaf roller or the tea black moth.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] The organic fertilizer of the present invention has readily available raw materials, abundant resources, and low cost, and comprehensively utilizes the Chinese medicinal plant resources and microbial resources widely existing in nature; the preparation method is simple, does not require large-scale mechanical equipment, and has good prospects for promotion and application; while improving the yield and quality of spring tea, it also has an insecticidal effect and can be used for the prevention and control of pests such as tea leaf rollers and tea black moths. DETAILED DESCRIPTION

[0033] The technical solution of the present invention will be further described in detail below in conjunction with specific implementation methods.

[0034] Example 1

[0035] An organic fertilizer suitable for increasing tea production and killing insects, comprising the following raw materials in parts by weight: 50 parts of straw, 30 parts of chicken manure, 15 parts of Tibetan woodruff, 15 parts of hyssopus herba, 12 parts of hyssopus cuneiformis, 10 parts of scutellaria baicalensis, 8 parts of ellagic acid-rice protein hydrolysate, 5 parts of sodium laurate-modified diatomaceous earth, 5 parts of sodium humate, 4 parts of urea, 1 part of composite microorganisms, and 200 parts of water;

[0036] The composite microorganism consists of Candida utilis and Neurospora intermedia at a mass ratio of 1:2.

[0037] The preparation method of the ellagic acid-rice protein hydrolysate comprises the following steps:

[0038] (1) Rice was crushed, and water was added and preheated to 40°C. The pH was adjusted to 9, and alkaline protease was added for enzymolysis for 200 min. After the enzymolysis was completed, the pH was adjusted to 8.5, and the enzyme was inactivated to obtain an enzymolysis solution; wherein the material-liquid ratio of rice to water was 20 g:100 mL.

[0039] (2) dissolving ellagic acid in anhydrous ethanol to obtain an ellagic acid solution; wherein the solid-liquid ratio of ellagic acid to anhydrous ethanol is 1 g:10 mL;

[0040] (3) The enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2) were mixed and stirred for 60 minutes to obtain ellagic acid-rice protein hydrolysate.

[0041] The preparation method of the sodium laurate modified diatomaceous earth comprises the following steps:

[0042] (a) adding diatomaceous earth to a 10 wt % sodium hydroxide aqueous solution and soaking it for 15 min, and filtering to obtain pretreated diatomaceous earth; wherein the material-liquid ratio of diatomaceous earth to sodium hydroxide aqueous solution is 1 g:3 mL;

[0043] (b) adding sodium laurate and water to the pretreated diatomaceous earth obtained in step (a), stirring for 10 min, cooling and drying, and then grinding to obtain sodium laurate-modified diatomaceous earth; wherein the material-liquid ratio of the pretreated diatomaceous earth, sodium laurate and water is 1 g:1.5 mL:20 mL.

[0044] A method for preparing an organic fertilizer suitable for increasing tea production and killing insects comprises the following steps:

[0045] S1, weigh the raw materials according to weight;

[0046] S2. First, crush the Tibetan wood root, the herb of wormwood, the herb of wormwood and the herb of black bone vine, then add sodium humate and water, mix, soak for 100 minutes, add urea, mix well, and obtain the fermentation product;

[0047] S3, adding straw, chicken manure and composite microorganisms to the fermented material obtained in step S2, performing aerobic fermentation at a temperature of 25° C. for 10 days, and drying to a moisture content of less than 12 wt % to obtain a fermentation product;

[0048] S4. Add ellagic acid-rice protein hydrolysate and sodium laurate modified diatomaceous earth to the fermentation product obtained in step S3, mix well, and granulate to obtain organic fertilizer.

[0049] Example 2

[0050] An organic fertilizer suitable for increasing tea production and killing insects, comprising the following raw materials in parts by weight: 70 parts of straw, 40 parts of chicken manure, 25 parts of Tibetan woodruff, 20 parts of hyssopus herba, 22 parts of hyssopus cuneiformis, 18 parts of scutellaria baicalensis, 15 parts of ellagic acid-rice protein hydrolysate, 10 parts of sodium laurate-modified diatomaceous earth, 10 parts of sodium humate, 15 parts of urea, 6 parts of composite microorganisms, and 300 parts of water;

[0051] The composite microorganisms are composed of Candida utilis and Neurospora intermedia at a mass ratio of 5:7.

[0052] The preparation method of the ellagic acid-rice protein hydrolysate comprises the following steps:

[0053] (1) Rice was crushed, and water was added and preheated to 50° C., the pH was adjusted to 11, and alkaline protease was added for enzymolysis for 300 min. After the enzymolysis was completed, the pH was adjusted to 9.6, and the enzyme was inactivated to obtain an enzymolysis solution; wherein the material-liquid ratio of rice to water was 30 g:100 mL;

[0054] (2) dissolving ellagic acid in anhydrous ethanol to obtain an ellagic acid solution; wherein the solid-liquid ratio of ellagic acid to anhydrous ethanol is 1 g:20 mL;

[0055] (3) The enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2) were mixed and stirred for 120 minutes to obtain ellagic acid-rice protein hydrolysate.

[0056] The preparation method of the sodium laurate modified diatomaceous earth comprises the following steps:

[0057] (a) adding diatomaceous earth to a 15 wt % sodium hydroxide aqueous solution and soaking it for 40 min, filtering to obtain pretreated diatomaceous earth; wherein the material-liquid ratio of diatomaceous earth to sodium hydroxide aqueous solution is 1 g:7 mL

[0058] (b) adding sodium laurate and water to the pretreated diatomaceous earth obtained in step (a), stirring for 15 minutes, cooling and drying, and then grinding to obtain sodium laurate-modified diatomaceous earth; wherein the material-liquid ratio of the pretreated diatomaceous earth, sodium laurate and water is 1 g:3 mL:50 mL.

[0059] A method for preparing an organic fertilizer suitable for increasing tea production and killing insects comprises the following steps:

[0060] S1, weigh the raw materials according to weight;

[0061] S2. First, crush the Tibetan wood root, the herb Yutuncao, the herb Acanthospermum officinale, and the black bone vine, then add sodium humate and water, mix, soak for 200 minutes, add urea, and mix well to obtain the fermentation product;

[0062] S3, adding straw, chicken manure and composite microorganisms to the fermented material obtained in step S2, performing aerobic fermentation at a temperature of 28° C. for 20 days, and drying to a water content of less than 20 wt % to obtain a fermentation product;

[0063] S4. Add ellagic acid-rice protein hydrolysate and sodium laurate modified diatomaceous earth to the fermentation product obtained in step S3, mix well, and granulate to obtain organic fertilizer.

[0064] Example 3

[0065] An organic fertilizer suitable for increasing tea production and killing insects, comprising the following raw materials in parts by weight: 60 parts of straw, 35 parts of chicken manure, 20 parts of Tibetan woodruff, 18 parts of hyssopus ulmoides, 15 parts of scutellaria baicalensis, 12 parts of scutellaria baicalensis, 12 parts of ellagic acid-rice protein hydrolysate, 8 parts of sodium laurate-modified diatomaceous earth, 7 parts of sodium humate, 6 parts of urea, 5 parts of composite microorganisms, and 280 parts of water;

[0066] The composite microorganism consists of Candida utilis and Neurospora intermedia at a mass ratio of 2:5.

[0067] The preparation method of the ellagic acid-rice protein hydrolysate comprises the following steps:

[0068] (1) Rice was crushed, and water was added and preheated to 45° C., the pH was adjusted to 10, and alkaline protease was added for enzymolysis for 250 min. After the enzymolysis was completed, the pH was adjusted to 9.0, and the enzyme was inactivated to obtain an enzymolysis solution; wherein the material-liquid ratio of rice to water was 27 g:100 mL;

[0069] (2) dissolving ellagic acid in anhydrous ethanol to obtain an ellagic acid solution; wherein the solid-liquid ratio of ellagic acid to anhydrous ethanol is 1 g:15 mL;

[0070] (3) The enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2) were mixed and stirred for 100 minutes to obtain ellagic acid-rice protein hydrolysate.

[0071] The preparation method of the sodium laurate modified diatomaceous earth comprises the following steps:

[0072] (a) adding diatomaceous earth to a 12 wt % sodium hydroxide aqueous solution and soaking it for 30 min, and filtering to obtain pretreated diatomaceous earth; wherein the material-liquid ratio of diatomaceous earth to sodium hydroxide aqueous solution is 1 g:5 mL;

[0073] (b) adding sodium laurate and water to the pretreated diatomaceous earth obtained in step (a), stirring for 12 minutes, cooling and drying, and then grinding to obtain sodium laurate-modified diatomaceous earth; wherein the material-liquid ratio of the pretreated diatomaceous earth, sodium laurate, and water is 1 g:2 mL:25 mL.

[0074] A method for preparing an organic fertilizer suitable for increasing tea production and killing insects comprises the following steps:

[0075] S1, weigh the raw materials according to weight;

[0076] S2. First, crush the herbs of Tibetan wood root, Herba scutellariae, Herba scutellariae and Herba scutellariae, then add sodium humate and water, soak for 150 minutes, add urea, and mix well to obtain the fermentation product;

[0077] S3, adding straw, chicken manure and composite microorganisms to the fermented material obtained in step S2, performing aerobic fermentation at a temperature of 27° C. for 18 days, and drying to a moisture content of less than 18 wt % to obtain a fermentation product;

[0078] S4. Add ellagic acid-rice protein hydrolysate and sodium laurate modified diatomaceous earth to the fermentation product obtained in step S3, mix well, and granulate to obtain organic fertilizer.

[0079] Comparative Example 1

[0080] This comparative example differs from Example 3 in that the raw material, Tibetan wood fragrance, is replaced by purslane, and the other ingredients and steps remain unchanged.

[0081] An organic fertilizer suitable for increasing tea production and killing insects, comprising the following raw materials in parts by weight: 60 parts of straw, 35 parts of chicken manure, 20 parts of purslane, 18 parts of hyssopus herba, 15 parts of scutellaria baicalensis, 12 parts of scutellaria baicalensis, 12 parts of ellagic acid-rice protein hydrolysate, 8 parts of sodium laurate-modified diatomaceous earth, 7 parts of sodium humate, 6 parts of urea, 5 parts of composite microorganisms, and 280 parts of water;

[0082] The composite microorganism consists of Candida utilis and Neurospora intermedia at a mass ratio of 2:5.

[0083] The preparation method of the ellagic acid-rice protein hydrolysate comprises the following steps:

[0084] (1) Rice was crushed, and water was added and preheated to 45° C., the pH was adjusted to 10, and alkaline protease was added for enzymolysis for 250 min. After the enzymolysis was completed, the pH was adjusted to 9.0, and the enzyme was inactivated to obtain an enzymolysis solution; wherein the material-liquid ratio of rice to water was 27 g:100 mL;

[0085] (2) dissolving ellagic acid in anhydrous ethanol to obtain an ellagic acid solution; wherein the solid-liquid ratio of ellagic acid to anhydrous ethanol is 1 g:15 mL;

[0086] (3) The enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2) were mixed and stirred for 100 minutes to obtain ellagic acid-rice protein hydrolysate.

[0087] The preparation method of the sodium laurate modified diatomaceous earth comprises the following steps:

[0088] (a) adding diatomaceous earth to a 12 wt % sodium hydroxide aqueous solution and soaking it for 30 min, and filtering to obtain pretreated diatomaceous earth; wherein the material-liquid ratio of diatomaceous earth to sodium hydroxide aqueous solution is 1 g:5 mL;

[0089] (b) adding sodium laurate and water to the pretreated diatomaceous earth obtained in step (a), stirring for 12 minutes, cooling and drying, and then grinding to obtain sodium laurate-modified diatomaceous earth; wherein the material-liquid ratio of the pretreated diatomaceous earth, sodium laurate, and water is 1 g:2 mL:25 mL.

[0090] A method for preparing an organic fertilizer suitable for increasing tea production and killing insects comprises the following steps:

[0091] S1, weigh the raw materials according to weight;

[0092] S2. First, crush purslane, wormwood, wormwood and black bone vine, then add sodium humate and water, soak for 150 minutes, then add urea, mix well, and obtain the fermentation product;

[0093] S3, adding straw, chicken manure and composite microorganisms to the fermented material obtained in step S2, performing aerobic fermentation at a temperature of 27° C. for 18 days, and drying to a moisture content of less than 18 wt % to obtain a fermentation product;

[0094] S4. Add ellagic acid-rice protein hydrolysate and sodium laurate modified diatomaceous earth to the fermentation product obtained in step S3, mix well, and granulate to obtain organic fertilizer.

[0095] Comparative Example 2

[0096] This comparative example differs from Example 3 in that the raw material Herba Achyranthis Bidens is replaced with Melia azedarach, and the other ingredients and steps remain unchanged.

[0097] An organic fertilizer suitable for increasing tea production and killing insects, comprising the following raw materials in parts by weight: 60 parts of straw, 35 parts of chicken manure, 20 parts of Tibetan woodruff, 18 parts of Chinaberry fruit, 15 parts of Artemisia argyi, 12 parts of Trichosanthes kirilowii, 12 parts of ellagic acid-rice protein hydrolysate, 8 parts of sodium laurate-modified diatomaceous earth, 7 parts of sodium humate, 6 parts of urea, 5 parts of composite microorganisms, and 280 parts of water;

[0098] The composite microorganism consists of Candida utilis and Neurospora intermedia at a mass ratio of 2:5.

[0099] The preparation method of the ellagic acid-rice protein hydrolysate comprises the following steps:

[0100] (1) Rice was crushed, and water was added and preheated to 45° C., the pH was adjusted to 10, and alkaline protease was added for enzymolysis for 250 min. After the enzymolysis was completed, the pH was adjusted to 9.0, and the enzyme was inactivated to obtain an enzymolysis solution; wherein the material-liquid ratio of rice to water was 27 g:100 mL;

[0101] (2) dissolving ellagic acid in anhydrous ethanol to obtain an ellagic acid solution; wherein the solid-liquid ratio of ellagic acid to anhydrous ethanol is 1 g:15 mL;

[0102] (3) The enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2) were mixed and stirred for 100 minutes to obtain ellagic acid-rice protein hydrolysate.

[0103] The preparation method of the sodium laurate modified diatomaceous earth comprises the following steps:

[0104] (a) adding diatomaceous earth to a 12 wt % sodium hydroxide aqueous solution and soaking it for 30 min, and filtering to obtain pretreated diatomaceous earth; wherein the material-liquid ratio of diatomaceous earth to sodium hydroxide aqueous solution is 1 g:5 mL;

[0105] (b) adding sodium laurate and water to the pretreated diatomaceous earth obtained in step (a), stirring for 12 minutes, cooling and drying, and then grinding to obtain sodium laurate-modified diatomaceous earth; wherein the material-liquid ratio of the pretreated diatomaceous earth, sodium laurate, and water is 1 g:2 mL:25 mL.

[0106] A method for preparing an organic fertilizer suitable for increasing tea production and killing insects comprises the following steps:

[0107] S1, weigh the raw materials according to weight;

[0108] S2. First, crush the Tibetan wood root, Chinaberry fruit, Artemisia annua, and Achyranthes bidentata, then add sodium humate and water, mix, soak for 150 minutes, add urea, and mix well to obtain a fermentation product;

[0109] S3, adding straw, chicken manure and composite microorganisms to the fermented material obtained in step S2, performing aerobic fermentation at a temperature of 27° C. for 18 days, and drying to a moisture content of less than 18 wt % to obtain a fermentation product;

[0110] S4. Add ellagic acid-rice protein hydrolysate and sodium laurate modified diatomaceous earth to the fermentation product obtained in step S3, mix well, and granulate to obtain organic fertilizer.

[0111] Comparative Example 3

[0112] This comparative example differs from Example 3 in that: there is no Artemisia selengensis in the raw materials, and the other ingredients and steps remain unchanged.

[0113] An organic fertilizer suitable for increasing tea production and killing insects, comprising the following raw materials in parts by weight: 60 parts of straw, 35 parts of chicken manure, 20 parts of Tibetan wood fragrance, 18 parts of euphorbia milii, 12 parts of smilax china, 12 parts of ellagic acid-rice protein hydrolysate, 8 parts of sodium laurate-modified diatomaceous earth, 7 parts of sodium humate, 6 parts of urea, 5 parts of composite microorganisms, and 280 parts of water;

[0114] The composite microorganism consists of Candida utilis and Neurospora intermedia at a mass ratio of 2:5.

[0115] The preparation method of the ellagic acid-rice protein hydrolysate comprises the following steps:

[0116] (1) Rice was crushed, and water was added and preheated to 45° C., the pH was adjusted to 10, and alkaline protease was added for enzymolysis for 250 min. After the enzymolysis was completed, the pH was adjusted to 9.0, and the enzyme was inactivated to obtain an enzymolysis solution; wherein the material-liquid ratio of rice to water was 27 g:100 mL;

[0117] (2) dissolving ellagic acid in anhydrous ethanol to obtain an ellagic acid solution; wherein the solid-liquid ratio of ellagic acid to anhydrous ethanol is 1 g:15 mL;

[0118] (3) The enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2) were mixed and stirred for 100 minutes to obtain ellagic acid-rice protein hydrolysate.

[0119] The preparation method of the sodium laurate modified diatomaceous earth comprises the following steps:

[0120] (a) adding diatomaceous earth to a 12 wt % sodium hydroxide aqueous solution and soaking it for 30 min, and filtering to obtain pretreated diatomaceous earth; wherein the material-liquid ratio of diatomaceous earth to sodium hydroxide aqueous solution is 1 g:5 mL;

[0121] (b) adding sodium laurate and water to the pretreated diatomaceous earth obtained in step (a), stirring for 12 minutes, cooling and drying, and then grinding to obtain sodium laurate-modified diatomaceous earth; wherein the material-liquid ratio of the pretreated diatomaceous earth, sodium laurate, and water is 1 g:2 mL:25 mL.

[0122] A method for preparing an organic fertilizer suitable for increasing tea production and killing insects comprises the following steps:

[0123] S1, weigh the raw materials according to weight;

[0124] S2. First, crush the Tibetan wood root, the herb of wormwood and the black bone vine, then add sodium humate and water, mix, soak for 150 minutes, add urea, mix well, and obtain the fermentation product;

[0125] S3, adding straw, chicken manure and composite microorganisms to the fermented material obtained in step S2, performing aerobic fermentation at a temperature of 27° C. for 18 days, and drying to a moisture content of less than 18 wt % to obtain a fermentation product;

[0126] S4. Add ellagic acid-rice protein hydrolysate and sodium laurate modified diatomaceous earth to the fermentation product obtained in step S3, mix well, and granulate to obtain organic fertilizer.

[0127] Comparative Example 4

[0128] This comparative example differs from Example 3 in that: there is no Achyranthes australis in the raw materials, and the other ingredients and steps remain unchanged.

[0129] An organic fertilizer suitable for increasing tea production and killing insects, comprising the following raw materials in parts by weight: 60 parts of straw, 35 parts of chicken manure, 20 parts of Tibetan woodruff, 18 parts of hyssopus ulmoides, 15 parts of hyssopus ulmoides, 12 parts of ellagic acid-rice protein hydrolysate, 8 parts of sodium laurate-modified diatomaceous earth, 7 parts of sodium humate, 6 parts of urea, 5 parts of composite microorganisms, and 280 parts of water;

[0130] The composite microorganism consists of Candida utilis and Neurospora intermedia at a mass ratio of 2:5.

[0131] The preparation method of the ellagic acid-rice protein hydrolysate comprises the following steps:

[0132] (1) Rice was crushed, and water was added and preheated to 45° C., the pH was adjusted to 10, and alkaline protease was added for enzymolysis for 250 min. After the enzymolysis was completed, the pH was adjusted to 9.0, and the enzyme was inactivated to obtain an enzymolysis solution; wherein the material-liquid ratio of rice to water was 27 g:100 mL;

[0133] (2) dissolving ellagic acid in anhydrous ethanol to obtain an ellagic acid solution; wherein the solid-liquid ratio of ellagic acid to anhydrous ethanol is 1 g:15 mL;

[0134] (3) The enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2) were mixed and stirred for 100 minutes to obtain ellagic acid-rice protein hydrolysate.

[0135] The preparation method of the sodium laurate modified diatomaceous earth comprises the following steps:

[0136] (a) adding diatomaceous earth to a 12 wt % sodium hydroxide aqueous solution and soaking it for 30 min, and filtering to obtain pretreated diatomaceous earth; wherein the material-liquid ratio of diatomaceous earth to sodium hydroxide aqueous solution is 1 g:5 mL;

[0137] (b) adding sodium laurate and water to the pretreated diatomaceous earth obtained in step (a), stirring for 12 minutes, cooling and drying, and then grinding to obtain sodium laurate-modified diatomaceous earth; wherein the material-liquid ratio of the pretreated diatomaceous earth, sodium laurate, and water is 1 g:2 mL:25 mL.

[0138] A method for preparing an organic fertilizer suitable for increasing tea production and killing insects comprises the following steps:

[0139] S1, weigh the raw materials according to weight;

[0140] S2. First, crush the Tibetan wood root, the herb of wormwood and the herb of wormwood, then add sodium humate and water, soak for 150 minutes, then add urea, mix well, and obtain the fermentation product;

[0141] S3, adding straw, chicken manure and composite microorganisms to the fermented material obtained in step S2, performing aerobic fermentation at a temperature of 27° C. for 18 days, and drying to a moisture content of less than 18 wt % to obtain a fermentation product;

[0142] S4. Add ellagic acid-rice protein hydrolysate and sodium laurate modified diatomaceous earth to the fermentation product obtained in step S3, mix well, and granulate to obtain organic fertilizer.

[0143] Comparative Example 5

[0144] This comparative example differs from Example 3 in that the raw material ellagic acid-rice protein hydrolysate is replaced with rice protein hydrolysate, and the other components and steps remain unchanged.

[0145] An organic fertilizer suitable for increasing tea production and killing insects, comprising the following raw materials in parts by weight: 60 parts of straw, 35 parts of chicken manure, 20 parts of Tibetan woodruff, 18 parts of hyssopus ulmoides, 15 parts of scutellaria baicalensis, 12 parts of scutellaria baicalensis, 12 parts of rice protein hydrolysate, 8 parts of sodium laurate-modified diatomaceous earth, 7 parts of sodium humate, 6 parts of urea, 5 parts of composite microorganisms, and 280 parts of water;

[0146] The composite microorganism consists of Candida utilis and Neurospora intermedia at a mass ratio of 2:5.

[0147] The method for preparing the rice protein hydrolysate comprises the following steps: grinding rice, adding water and preheating to 45° C., adjusting the pH to 10, adding alkaline protease and performing enzymatic hydrolysis for 250 min, adjusting the pH to 9.0 after the enzymatic hydrolysis is completed, and inactivating the enzyme to obtain the rice protein hydrolysate; wherein the material-liquid ratio of rice to water is 27 g:100 mL;

[0148] The preparation method of the sodium laurate modified diatomaceous earth comprises the following steps:

[0149] (a) adding diatomaceous earth to a 12 wt % sodium hydroxide aqueous solution and soaking it for 30 min, and filtering to obtain pretreated diatomaceous earth; wherein the material-liquid ratio of diatomaceous earth to sodium hydroxide aqueous solution is 1 g:5 mL;

[0150] (b) adding sodium laurate and water to the pretreated diatomaceous earth obtained in step (a), stirring for 12 minutes, cooling and drying, and then grinding to obtain sodium laurate-modified diatomaceous earth; wherein the material-liquid ratio of the pretreated diatomaceous earth, sodium laurate, and water is 1 g:2 mL:25 mL.

[0151] A method for preparing an organic fertilizer suitable for increasing tea production and killing insects comprises the following steps:

[0152] S1, weigh the raw materials according to weight;

[0153] S2. First, crush the herbs of Tibetan wood root, Herba scutellariae, Herba scutellariae and Herba scutellariae, then add sodium humate and water, soak for 150 minutes, add urea, and mix well to obtain the fermentation product;

[0154] S3, adding straw, chicken manure and composite microorganisms to the fermented material obtained in step S2, performing aerobic fermentation at a temperature of 27° C. for 18 days, and drying to a moisture content of less than 18 wt % to obtain a fermentation product;

[0155] S4. Add rice protein hydrolysate and sodium laurate modified diatomaceous earth to the fermentation product obtained in step S3, mix well, and granulate to obtain organic fertilizer.

[0156] Comparative Example 6

[0157] This comparative example differs from Example 3 in that the raw materials do not contain ellagic acid-rice protein hydrolysate, and the other components and steps remain unchanged.

[0158] An organic fertilizer suitable for increasing tea production and killing insects, comprising the following raw materials in parts by weight: 60 parts of straw, 35 parts of chicken manure, 20 parts of Tibetan wood fragrance, 18 parts of hyssopus ulmoides, 15 parts of scutellaria baicalensis, 12 parts of scutellaria baicalensis, 8 parts of sodium laurate-modified diatomaceous earth, 7 parts of sodium humate, 6 parts of urea, 5 parts of composite microorganisms, and 280 parts of water;

[0159] The composite microorganism consists of Candida utilis and Neurospora intermedia at a mass ratio of 2:5.

[0160] The preparation method of the sodium laurate modified diatomaceous earth comprises the following steps:

[0161] (a) adding diatomaceous earth to a 12 wt % sodium hydroxide aqueous solution and soaking it for 30 min, and filtering to obtain pretreated diatomaceous earth; wherein the material-liquid ratio of diatomaceous earth to sodium hydroxide aqueous solution is 1 g:5 mL;

[0162] (b) adding sodium laurate and water to the pretreated diatomaceous earth obtained in step (a), stirring for 12 minutes, cooling and drying, and then grinding to obtain sodium laurate-modified diatomaceous earth; wherein the material-liquid ratio of the pretreated diatomaceous earth, sodium laurate, and water is 1 g:2 mL:25 mL.

[0163] A method for preparing an organic fertilizer suitable for increasing tea production and killing insects comprises the following steps:

[0164] S1, weigh the raw materials according to weight;

[0165] S2. First, crush the herbs of Tibetan wood root, Herba scutellariae, Herba scutellariae and Herba scutellariae, then add sodium humate and water, soak for 150 minutes, add urea, and mix well to obtain the fermentation product;

[0166] S3, adding straw, chicken manure and composite microorganisms to the fermented material obtained in step S2, performing aerobic fermentation at a temperature of 27° C. for 18 days, and drying to a moisture content of less than 18 wt % to obtain a fermentation product;

[0167] S4. Add sodium laurate-modified diatomaceous earth to the fermentation product obtained in step S3, mix evenly, and granulate to obtain an organic fertilizer.

[0168] Comparative Example 7

[0169] This comparative example differs from Example 3 in that the sodium laurate-modified diatomaceous earth is replaced by diatomaceous earth, and the other components and steps remain unchanged.

[0170] An organic fertilizer suitable for increasing tea production and killing insects, comprising the following raw materials in parts by weight: 60 parts of straw, 35 parts of chicken manure, 20 parts of Tibetan woodruff, 18 parts of hyssopus ulmoides, 15 parts of scutellaria baicalensis, 12 parts of scutellaria baicalensis, 12 parts of ellagic acid-rice protein hydrolysate, 8 parts of diatomaceous earth, 7 parts of sodium humate, 6 parts of urea, 5 parts of composite microorganisms, and 280 parts of water;

[0171] The composite microorganism consists of Candida utilis and Neurospora intermedia at a mass ratio of 2:5.

[0172] The preparation method of the ellagic acid-rice protein hydrolysate comprises the following steps:

[0173] (1) Rice was crushed, and water was added and preheated to 45° C., the pH was adjusted to 10, and alkaline protease was added for enzymolysis for 250 min. After the enzymolysis was completed, the pH was adjusted to 9.0, and the enzyme was inactivated to obtain an enzymolysis solution; wherein the material-liquid ratio of rice to water was 27 g:100 mL;

[0174] (2) dissolving ellagic acid in anhydrous ethanol to obtain an ellagic acid solution; wherein the solid-liquid ratio of ellagic acid to anhydrous ethanol is 1 g:15 mL;

[0175] (3) The enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2) were mixed and stirred for 100 minutes to obtain ellagic acid-rice protein hydrolysate.

[0176] A method for preparing an organic fertilizer suitable for increasing tea production and killing insects comprises the following steps:

[0177] S1, weigh the raw materials according to weight;

[0178] S2. First, crush the herbs of Tibetan wood root, Herba scutellariae, Herba scutellariae and Herba scutellariae, then add sodium humate and water, soak for 150 minutes, add urea, and mix well to obtain the fermentation product;

[0179] S3, adding straw, chicken manure and composite microorganisms to the fermented material obtained in step S2, performing aerobic fermentation at a temperature of 27° C. for 18 days, and drying to a moisture content of less than 18 wt % to obtain a fermentation product;

[0180] S4. Add ellagic acid-rice protein hydrolysate and diatomaceous earth to the fermentation product obtained in step S3, mix evenly, and granulate to obtain organic fertilizer.

[0181] Comparative Example 8

[0182] This comparative example differs from Example 3 in that the raw materials do not include sodium laurate-modified diatomaceous earth, and the other components and steps remain unchanged.

[0183] An organic fertilizer suitable for increasing tea production and killing insects, comprising the following raw materials in parts by weight: 60 parts of straw, 35 parts of chicken manure, 20 parts of Tibetan woodruff, 18 parts of hyssopus ulmoides, 15 parts of scutellaria baicalensis, 12 parts of scutellaria baicalensis, 12 parts of ellagic acid-rice protein hydrolysate, 7 parts of sodium humate, 6 parts of urea, 5 parts of composite microorganisms, and 280 parts of water;

[0184] The composite microorganism consists of Candida utilis and Neurospora intermedia at a mass ratio of 2:5.

[0185] The preparation method of the ellagic acid-rice protein hydrolysate comprises the following steps:

[0186] (1) Rice was crushed, and water was added and preheated to 45° C., the pH was adjusted to 10, and alkaline protease was added for enzymolysis for 250 min. After the enzymolysis was completed, the pH was adjusted to 9.0, and the enzyme was inactivated to obtain an enzymolysis solution; wherein the material-liquid ratio of rice to water was 27 g:100 mL;

[0187] (2) dissolving ellagic acid in anhydrous ethanol to obtain an ellagic acid solution; wherein the solid-liquid ratio of ellagic acid to anhydrous ethanol is 1 g:15 mL;

[0188] (3) The enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2) were mixed and stirred for 100 minutes to obtain ellagic acid-rice protein hydrolysate.

[0189] A method for preparing an organic fertilizer suitable for increasing tea production and killing insects comprises the following steps:

[0190] S1, weigh the raw materials according to weight;

[0191] S2. First, crush the herbs of Tibetan wood root, Herba scutellariae, Herba scutellariae and Herba scutellariae, then add sodium humate and water, soak for 150 minutes, add urea, and mix well to obtain the fermentation product;

[0192] S3, adding straw, chicken manure and composite microorganisms to the fermented material obtained in step S2, performing aerobic fermentation at a temperature of 27° C. for 18 days, and drying to a moisture content of less than 18 wt % to obtain a fermentation product;

[0193] S4. Add ellagic acid-rice protein hydrolysate to the fermentation product obtained in step S3, mix evenly, and granulate to obtain organic fertilizer.

[0194] Comparative Example 9

[0195] This comparative example differs from Example 3 in that the Candida utilis in the composite microbial bacteria as a raw material is replaced with Bacillus subtilis, and the other components and steps remain unchanged.

[0196] An organic fertilizer suitable for increasing tea production and killing insects, comprising the following raw materials in parts by weight: 60 parts of straw, 35 parts of chicken manure, 20 parts of Tibetan woodruff, 18 parts of hyssopus ulmoides, 15 parts of scutellaria baicalensis, 12 parts of scutellaria baicalensis, 12 parts of ellagic acid-rice protein hydrolysate, 8 parts of sodium laurate-modified diatomaceous earth, 7 parts of sodium humate, 6 parts of urea, 5 parts of composite microorganisms, and 280 parts of water;

[0197] The composite microorganism consists of Bacillus subtilis and Neurospora intermedia at a mass ratio of 2:5.

[0198] The preparation method of the ellagic acid-rice protein hydrolysate comprises the following steps:

[0199] (1) Rice was crushed, and water was added and preheated to 45° C., the pH was adjusted to 10, and alkaline protease was added for enzymolysis for 250 min. After the enzymolysis was completed, the pH was adjusted to 9.0, and the enzyme was inactivated to obtain an enzymolysis solution; wherein the material-liquid ratio of rice to water was 27 g:100 mL;

[0200] (2) dissolving ellagic acid in anhydrous ethanol to obtain an ellagic acid solution; wherein the solid-liquid ratio of ellagic acid to anhydrous ethanol is 1 g:15 mL;

[0201] (3) The enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2) were mixed and stirred for 100 minutes to obtain ellagic acid-rice protein hydrolysate.

[0202] The preparation method of the sodium laurate modified diatomaceous earth comprises the following steps:

[0203] (a) adding diatomaceous earth to a 12 wt % sodium hydroxide aqueous solution and soaking it for 30 min, and filtering to obtain pretreated diatomaceous earth; wherein the material-liquid ratio of diatomaceous earth to sodium hydroxide aqueous solution is 1 g:5 mL;

[0204] (b) adding sodium laurate and water to the pretreated diatomaceous earth obtained in step (a), stirring for 12 minutes, cooling and drying, and then grinding to obtain sodium laurate-modified diatomaceous earth; wherein the material-liquid ratio of the pretreated diatomaceous earth, sodium laurate, and water is 1 g:2 mL:25 mL.

[0205] A method for preparing an organic fertilizer suitable for increasing tea production and killing insects comprises the following steps:

[0206] S1, weigh the raw materials according to weight;

[0207] S2. First, crush the herbs of Tibetan wood root, Herba scutellariae, Herba scutellariae and Herba scutellariae, then add sodium humate and water, soak for 150 minutes, add urea, and mix well to obtain the fermentation product;

[0208] S3, adding straw, chicken manure and composite microorganisms to the fermented material obtained in step S2, performing aerobic fermentation at a temperature of 27° C. for 18 days, and drying to a moisture content of less than 18 wt % to obtain a fermentation product;

[0209] S4. Add ellagic acid-rice protein hydrolysate and sodium laurate modified diatomaceous earth to the fermentation product obtained in step S3, mix well, and granulate to obtain organic fertilizer.

[0210] Comparative Example 10

[0211] This comparative example differs from Example 3 in that Neurospora intermedia in the raw composite microorganism is replaced with Trichoderma acanthosporium, and the other components and steps remain unchanged.

[0212] An organic fertilizer suitable for increasing tea production and killing insects, comprising the following raw materials in parts by weight: 60 parts of straw, 35 parts of chicken manure, 20 parts of Tibetan woodruff, 18 parts of hyssopus ulmoides, 15 parts of scutellaria baicalensis, 12 parts of scutellaria baicalensis, 12 parts of ellagic acid-rice protein hydrolysate, 8 parts of sodium laurate-modified diatomaceous earth, 7 parts of sodium humate, 6 parts of urea, 5 parts of composite microorganisms, and 280 parts of water;

[0213] The composite microorganism consists of Candida utilis and Trichoderma aculeatus in a mass ratio of 2:5.

[0214] The preparation method of the ellagic acid-rice protein hydrolysate comprises the following steps:

[0215] (1) Rice was crushed, and water was added and preheated to 45° C., the pH was adjusted to 10, and alkaline protease was added for enzymolysis for 250 min. After the enzymolysis was completed, the pH was adjusted to 9.0, and the enzyme was inactivated to obtain an enzymolysis solution; wherein the material-liquid ratio of rice to water was 27 g:100 mL;

[0216] (2) dissolving ellagic acid in anhydrous ethanol to obtain an ellagic acid solution; wherein the solid-liquid ratio of ellagic acid to anhydrous ethanol is 1 g:15 mL;

[0217] (3) The enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2) were mixed and stirred for 100 minutes to obtain ellagic acid-rice protein hydrolysate.

[0218] The preparation method of the sodium laurate modified diatomaceous earth comprises the following steps:

[0219] (a) adding diatomaceous earth to a 12 wt % sodium hydroxide aqueous solution and soaking it for 30 min, and filtering to obtain pretreated diatomaceous earth; wherein the material-liquid ratio of diatomaceous earth to sodium hydroxide aqueous solution is 1 g:5 mL;

[0220] (b) adding sodium laurate and water to the pretreated diatomaceous earth obtained in step (a), stirring for 12 minutes, cooling and drying, and then grinding to obtain sodium laurate-modified diatomaceous earth; wherein the material-liquid ratio of the pretreated diatomaceous earth, sodium laurate, and water is 1 g:2 mL:25 mL.

[0221] A method for preparing an organic fertilizer suitable for increasing tea production and killing insects comprises the following steps:

[0222] S1, weigh the raw materials according to weight;

[0223] S2. First, crush the herbs of Tibetan wood root, Herba scutellariae, Herba scutellariae and Herba scutellariae, then add sodium humate and water, soak for 150 minutes, add urea, and mix well to obtain the fermentation product;

[0224] S3, adding straw, chicken manure and composite microorganisms to the fermented material obtained in step S2, performing aerobic fermentation at a temperature of 27° C. for 18 days, and drying to a moisture content of less than 18 wt % to obtain a fermentation product;

[0225] S4. Add ellagic acid-rice protein hydrolysate and sodium laurate modified diatomaceous earth to the fermentation product obtained in step S3, mix well, and granulate to obtain organic fertilizer.

[0226] Test Example 1: Increasing tea yield

[0227] 1.1 Test method

[0228] A tea garden harvesting only spring Huangshan Maofeng tea was selected and divided into 42 plots of equal area, each measuring 4 mu (approximately 1.5 acres). The plots were divided into experimental groups (Examples 1-3), comparative groups (Comparative Examples 1-10), and a control group (conventional fertilizer: N:P₂O₅:K₂O:MgO = 22:8:10:2). Three parallel experiments were conducted in each group. The yield of each harvest was recorded after harvesting. The contents of free amino acids (determined using the ninhydrin colorimetric method (GB / T8314-2002)), tea polyphenols (determined using the ferric tartrate colorimetric method (GB / T8313-2002)), and caffeine (determined using ultraviolet spectrophotometry (GB / T8312-2002)) were also measured. The results are shown in Table 1.

[0229] 1.2 Tea production and quality

[0230] Table 1 Average tea yield and tea quality

[0231] Average fresh tea yield per mu / kg Free amino acids / % Tea polyphenols% Caffeine% control group 41.3 2.1 21.2 2.0 Example 1 64.5 4.4 25.0 4.1 Example 2 65.0 4.5 25.1 4.2 Example 3 65.2 4.5 25.3 4.5 Comparative Example 1 58.6 4.3 24.8 3.7 Comparative Example 2 60.1 4.3 25.0 3.9 Comparative Example 3 44.2 3.2 22.6 2.6 Comparative Example 4 43.8 2.9 22.0 2.6 Comparative Example 5 50.1 3.7 23.5 2.8 Comparative Example 6 47.0 3.4 22.8 2.7 Comparative Example 7 51.7 3.8 24.0 3.1 Comparative Example 8 47.9 3.5 23.0 2.7 Comparative Example 9 55.1 4.1 24.6 3.6 Comparative Example 10 54.4 3.9 24.3 3.4

[0232] As can be seen from Table 1, after using the organic fertilizers of Examples 1 to 3 of the present invention, the average fresh tea yield per mu, free amino acid content, tea polyphenol content and caffeine content are all optimal. Compared with Comparative Examples 1 to 4, Example 3 shows that the mutual compatibility of Tibetan Wood Rhizome, Wormwood Herb, Curcuma Herba and Black Bone Twig plays a very important role in improving the yield and quality of tea leaves in organic fertilizers. The lack of any raw material or the replacement of raw materials will lead to a decrease in the effect. Compared with Comparative Examples 5 to 8, Example 3 shows that the replacement of ellagic acid-rice protein hydrolysate with rice protein hydrolysate and the replacement of sodium laurate modified diatomaceous earth with diatomaceous earth will cause a decrease in tea yield. When ellagic acid is combined with rice protein hydrolysate, it can synergistically enhance the stability of organic fertilizers and maintain the biological activity of ellagic acid. Diatomaceous earth, through surface modification, further improves the adsorption capacity of diatomaceous earth, optimizes the release of nutrients in the soil, maintains the dynamic balance of soil fertility, and thus improves the absorption efficiency and growth rate of tea roots; Comparison of Example 3 with Comparative Examples 9 and 10 shows that the selection of suitable aerobic bacteria is particularly important. Candida utilis can decompose macromolecular compounds such as protein and cellulose and convert them into small molecular substances such as amino acids and monosaccharides. Neurospora intermedia accelerates the degradation of raw materials in organic fertilizers by secreting extracellular enzymes, releasing active ingredients, thereby improving the soil fertility environment and the tea growth environment.

[0233] Test Example 2 Insecticide Effect

[0234] 2.1 Test Materials: The organic fertilizers prepared in Examples 1 to 3 were mixed with water at a material-liquid ratio of 1 g:3 mL, stirred for 20 min, and then filtered to obtain an organic fertilizer aqueous solution, which was used for insecticidal effect testing; 2.5% deltamethrin.

[0235] 2.2 Test method: Determination of contact activity against tea leaf roller and tea black tussock moth

[0236] Methods: The leaf disc method was used. Fresh, untreated Huangshan Maofeng leaves were perforated to create circular leaf discs with a diameter of 10 cm. The leaves were immersed in the test material for 5 minutes and then air-dried. Thirty tea leaf rollers and black tussock moths were inoculated into the discs and placed in an incubator with a light / dark cycle of 15 h / 8 h, a temperature of 25°C, and a humidity of 60-70%. The experimental group consisted of leaves immersed in the aqueous solution of organic fertilizer prepared in this application. The blank control group consisted of leaves immersed in clean water. The positive control group consisted of leaves immersed in 2.5% deltamethrin. Each treatment was replicated three times. After 24 hours of treatment, the mortality and survival of tea leaf rollers and black tussock moths in each treatment were examined, and the mortality rate was calculated.

[0237] (3) Test results

[0238] As shown in Table 2 and Table 3:

[0239] Table 2 Results of contact activity test against tea leaf roller

[0240] Serial number Number of survivors fatality rate Example 1 3 90.00％ Example 2 3 90.00％ Example 3 2 93.33％ Positive control group 5 83.33％ Blank control group 30 0.00％

[0241] Table 3 Results of contact activity test on black tussock moth

[0242] Serial number Number of survivors fatality rate Example 1 6 80.00％ Example 2 6 80.00％ Example 3 4 86.67％ Positive control group 8 73.33％ Blank control group 30 0.00％

[0243] As can be seen from the above table, the mortality rates of the organic fertilizer aqueous solutions prepared in Examples 1 to 3 against tea leaf rollers and tea black tussock moths were all 80% or above, among which Example 3 had the best insecticidal effect. Compared with the positive control group, Example 3 showed that the insecticide of the present invention was more effective than the commonly used and recognized insecticide deltamethrin in the art.

[0244] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. An organic fertilizer suitable for increasing tea production and killing insects, characterized in that: The invention comprises the following raw materials in parts by weight: 50-70 parts of straw, 30-40 parts of chicken manure, 15-25 parts of Tibetan wood fragrance, 15-20 parts of hyssopus herba, 12-22 parts of scutellaria baicalensis, 10-18 parts of scutellaria baicalensis, 8-15 parts of ellagic acid-rice protein hydrolysate, 5-10 parts of sodium laurate modified diatomaceous earth, 5-10 parts of sodium humate, 4-15 parts of urea, 1-6 parts of composite microorganisms and 200-300 parts of water; The composite microorganisms are composed of Candida utilis and Neurospora intermedia in a mass ratio of (1-5): (2-7).

2. An organic fertilizer suitable for increasing tea production and killing insects according to claim 1, characterized in that, The invention comprises the following raw materials in parts by weight: 60 parts of straw, 35 parts of chicken manure, 20 parts of Tibetan wood fragrance, 18 parts of hyssopus herba, 15 parts of kohlrabi herba, 12 parts of scutellaria baicalensis, 12 parts of ellagic acid-rice protein hydrolysate, 8 parts of sodium laurate modified diatomaceous earth, 7 parts of sodium humate, 6 parts of urea, 5 parts of composite microorganisms and 280 parts of water; The composite microorganism consists of Candida utilis and Neurospora intermedia at a mass ratio of 2:

5.

3. An organic fertilizer suitable for increasing tea production and killing insects according to claim 1, characterized in that, The preparation method of the ellagic acid-rice protein hydrolysate comprises the following steps: (1) Rice is crushed, and water is added and preheated to 40-50° C., the pH is adjusted to 9-11, and alkaline protease is added for enzymolysis for 200-300 min. After the enzymolysis is completed, the pH is adjusted to 8.5-9.6, and the enzyme is inactivated to obtain an enzymolysis solution; (2) dissolving ellagic acid in anhydrous ethanol to obtain an ellagic acid solution; (3) The enzymatic hydrolysate obtained in step (1) and the ellagic acid solution obtained in step (2) are mixed and stirred for 60 to 120 minutes to obtain ellagic acid-rice protein hydrolysate.

4. An organic fertilizer suitable for increasing tea production and killing insects according to claim 3, characterized in that, In step (1), the material-liquid ratio of rice to water is (20-30) g:100 mL.

5. An organic fertilizer suitable for increasing tea production and killing insects according to claim 3, characterized in that, In step (2), the material-liquid ratio of ellagic acid to anhydrous ethanol is 1 g: (10-20) mL.

6. An organic fertilizer suitable for increasing tea production and killing insects according to claim 1, characterized in that: The preparation method of the sodium laurate modified diatomaceous earth comprises the following steps: (a) adding diatomaceous earth to a 10-15 wt% sodium hydroxide aqueous solution and soaking it for 15-40 minutes at a material-liquid ratio of 1 g: (3-7) mL, and filtering to obtain pretreated diatomaceous earth; (b) adding sodium laurate and water to the pretreated diatomaceous earth obtained in step (a), stirring for 10 to 15 minutes, cooling and drying, and then grinding to obtain sodium laurate-modified diatomaceous earth.

7. An organic fertilizer suitable for increasing tea production and killing insects according to claim 6, characterized in that: In step (b), the material-liquid ratio of the pretreated diatomaceous earth, sodium laurate and water is 1 g: (1.5-3) mL: (20-50) mL.

8. A method for preparing an organic fertilizer suitable for increasing tea production and killing insects according to any one of claim 1, characterized in that: The following steps are involved: S1. Weigh the raw materials according to the weight ratio of claim 1; S2. First, crush the Tibetan wood fragrance, the herb of yutun, the herb of guqin and the vine of fujian, then add sodium humate and water, soak for 100 to 200 minutes, then add urea, mix well, and obtain the fermentation product; S3, adding straw, chicken manure and composite microorganisms to the fermented material obtained in step S2, performing aerobic fermentation at a temperature of 25-28° C. for 10-20 days, and drying to a moisture content of less than 12-20 wt % to obtain a fermentation product; S4. Add ellagic acid-rice protein hydrolysate and sodium laurate modified diatomaceous earth to the fermentation product obtained in step S3, mix well, and granulate to obtain organic fertilizer.

9. Use of the organic fertilizer according to any one of claims 1 to 7 or the organic fertilizer prepared by the preparation method according to claim 8 in improving the yield and quality of spring tea and preventing and / or killing tea pests.

10. The use according to claim 9, characterized in that The tea pests are tea leaf rollers or tea black moths.