Filtering, adsorbing and concentrating equipment for duck virus sample

Abstract

The invention relates to the technical field of molecular biology, in particular to duck virus sample filtering, adsorbing and concentrating equipment which comprises a box body, and a filtering assembly, a centrifugal assembly, an auxiliary assembly, a concentrating assembly, an air exhaust assembly and a driving assembly are arranged in the box body. The driving assembly comprises a controller and a driving piece, the driving piece is fixedly connected into the box body, and an output shaft of the driving piece is coaxially and fixedly connected with a rotating rod. A threaded sleeve is fixedly connected to the rotating rod and engaged with a gear. A baffle is fixedly connected in the box, an electromagnet is rotationally matched in the baffle, and the end, away from the driving piece, of the rotating rod penetrates through the baffle and is fixedly connected with the electromagnet. The driving piece drives the centrifugal barrel to rotate, and meanwhile the elastic balls are driven to promote the filtering effect of the filtering plate. The centrifuged duck viruses are concentrated through the concentration assembly, a plurality of steps are carried out in sequence, and a worker does not need to transfer duck virus samples for multiple times, so that the accuracy and reliability of duck virus detection are improved.

Description

Technical Field

[0001] The invention relates to the technical field of molecular biology, and in particular to a device for filtering, adsorbing and concentrating duck virus samples. Background Art

[0002] At present, with the increase in duck breeding volume, expansion of scale, diversification of models, and the increasing frequency of introduction of new species, import and export trade, poultry trading, and live poultry trading, new diseases have frequently occurred before old diseases have been eliminated, especially viral infectious diseases have continued to emerge, resulting in an increasingly severe situation of duck disease prevention and control, causing serious economic losses to farmers.

[0003] Generally speaking, when processing duck virus samples, staff need to remove impurities within the duck virus samples and concentrate the duck virus samples to increase the antigen content and immunogenicity of the vaccine. However, during each step of the processing process, the duck virus samples need to be processed separately. At the same time, due to factors such as the low viral load during the prodromal period and the detection limit of the test kit, the accuracy and reliability of subsequent duck virus testing are reduced, affecting the precise monitoring and prevention and control of the epidemic. High-purity duck virus samples help researchers to further study the structure, characteristics, and transmission mechanism of the virus, providing a scientific basis for the development of more effective prevention and control measures.

[0004] In summary, how to solve the problem of reduced accuracy and reliability of duck virus detection caused by separate processing of duck virus samples in each step has become a technical challenge that technicians in this field urgently need to solve. Therefore, it is necessary to propose a device for filtering, adsorbing and concentrating duck virus samples. Summary of the Invention

[0005] To address these issues, the present invention provides a device for filtering, adsorbing, and concentrating duck virus samples. A driving element drives the centrifuge barrel to rotate, simultaneously driving elastic balls to promote the filtration of the filter plate. The centrifuged duck virus is concentrated by a concentrating assembly. This sequential, multi-step process eliminates the need for workers to repeatedly transfer duck virus samples, thereby improving the accuracy and reliability of duck virus testing.

[0006] In order to achieve the above-mentioned purpose, the technical solution of the present invention is as follows: a device for filtering, adsorbing and concentrating duck virus samples, comprising a box body, a feed inlet at the top of the box body, a discharge port and a through-hole at the bottom of the box body, and a filtering component for filtering duck virus samples, a centrifugal component for centrifuging duck virus samples, an auxiliary component for improving the filtering effect, a concentrating component for concentrating duck virus samples, an exhaust component for exhausting the concentration component, and a driving component for driving the centrifugal component, the auxiliary component and the exhaust component.

[0007] The drive assembly includes a controller and a driver. The controller controls the operation of the driver, which is fixedly connected to the housing. The driver's output shaft is coaxially fixedly connected to a rotating rod. A threaded sleeve is fixedly connected to the rotating rod, which engages a gear. A fixed rod is fixedly connected to one side of the gear. The end of the fixed rod, away from the gear, rotates with the inner wall of the housing. A baffle is fixedly connected to the housing, and an electromagnet rotates in the baffle. The controller controls the opening and closing of the electromagnet. The end of the rotating rod, away from the driver, passes through the baffle and is fixedly connected to the electromagnet. The rotating rod rotates with the baffle. The electromagnet is magnetically connected to the centrifugal assembly.

[0008] The technical principle of the above scheme is as follows: the driving member drives the rotating rod to rotate, and the rotation of the rotating rod drives the rotation of the threaded sleeve, thereby driving the rotation of the gear, and the rotation of the gear drives the auxiliary component to assist in filtering the filter component, and drives the exhaust component to exhaust the concentration component.

[0009] The controller controls the opening and closing of the electromagnet, thereby controlling the rotation of the centrifugal assembly, thereby centrifuging the duck virus sample and separating the different components in the sample.

[0010] The above scheme has the following beneficial effects:

[0011] 1. The present invention uses a driving component to drive the centrifugal component, auxiliary component and exhaust component to start movement, thereby realizing continuous and efficient processing of duck virus samples from entering the box to concentration and discharge, greatly improving processing efficiency and reducing the risk of contamination during manual operation and sample transfer.

[0012] 2. The present invention promotes the filtering effect of the filtering component by continuously knocking the filtering component with the auxiliary component, thereby ensuring the continuity and high efficiency of the filtering.

[0013] 3. The present invention promotes the concentration of duck virus samples by creating a negative pressure within the concentration assembly through the vacuum assembly. This negative pressure concentration method can achieve rapid concentration of duck virus samples at a relatively low temperature, avoiding the destruction of active ingredients in the duck virus samples due to high temperatures.

[0014] Furthermore, the filter assembly includes a filter plate, which is fixedly connected to the box body.

[0015] Beneficial effects: Filtering the duck virus sample through the filter plate can filter out impurities in the duck virus sample and improve the purity of the duck virus sample.

[0016] Furthermore, the centrifuge assembly includes a centrifuge bucket rotatably connected to the baffle, the centrifuge bucket being positioned below the filter plate, and the bottom of the centrifuge bucket being magnetically connected to the electromagnet. A centrifugal opening is defined at the bottom of the centrifuge bucket, the centrifugal opening being fixedly connected to a delivery pipe, the end of the delivery pipe remote from the centrifugal opening being fixedly connected to the concentrating assembly.

[0017] Beneficial Effects: Through the centrifugal action of the centrifuge bucket, different components in the duck virus sample are effectively separated based on their density differences. The magnetic connection ensures the stability of the centrifuge bucket during high-speed rotation, avoiding loosening and shaking that may occur due to mechanical connections, ensuring a smooth centrifugation process.

[0018] Furthermore, the auxiliary component includes a fixed wheel eccentrically fixedly connected to the gear, a connecting plate is rotatably arranged on the outer periphery of the fixed wheel, an opening for the connecting plate to move is opened on the baffle, the connecting plate passes through the opening and slides with the opening, and an elastic ball is fixedly connected to the end of the connecting plate away from the fixed wheel, and the elastic ball is in contact with the filter plate.

[0019] Beneficial effect: The eccentric rotation of the fixed wheel drives the connecting plate to move up and down continuously, which in turn drives the elastic ball to continuously impact the filter plate. This continuous impact action can prevent impurities in the duck virus sample from accumulating on the filter plate surface and clogging it.

[0020] Furthermore, the vacuum assembly includes a push rod hingedly connected to a fixed wheel. A piston cylinder is fixedly connected to the bottom wall of the housing. The piston cylinder has an air inlet and an air outlet, each of which is fixedly connected to a one-way valve. The air inlet is fixedly connected to an air extraction pipeline, the end of which, remote from the air inlet, is fixedly connected to the concentration assembly. The air outlet is connected to a through hole. A piston head slidably fits within the piston cylinder, and the end of the piston head, remote from the air inlet, is hingedly connected to the push rod.

[0021] Beneficial effects: The concentration component is evacuated through the piston cylinder to form a negative pressure environment. This negative pressure environment helps the liquid in the duck virus sample to evaporate quickly, thereby concentrating the sample and greatly improving the concentration efficiency.

[0022] Furthermore, the concentrator assembly includes a concentrator tank fixedly connected to the tank body, with a delivery pipe and an air extraction pipe connected to the concentrator tank. A liquid outlet is formed at the bottom of the concentrator tank, which is connected to a material outlet. A solenoid valve is fixedly connected to the material outlet, and a controller is used to control the opening and closing of the solenoid valve.

[0023] Beneficial Effects: By pumping air through the exhaust pipe to create a negative pressure environment, the water in the liquid evaporates, thereby concentrating the sample. This centralized processing method can enrich the dispersed virus particles together, increasing the concentration of the virus in the sample and providing more valuable samples for subsequent analysis and research.

[0024] Furthermore, the filter plate is inclined.

[0025] Beneficial effects: Through the inclined filter plate, the duck virus sample can flow naturally under the action of gravity, which increases the speed at which the duck virus sample passes through the filter plate, thereby improving the overall filtration efficiency.

[0026] Furthermore, a transparent window is fixedly connected to the box body.

[0027] Beneficial effects: Through the transparent window, staff can clearly observe the processing flow of duck virus samples, which improves the accuracy and safety of duck virus processing.

[0028] Furthermore, a plurality of heat dissipation holes are opened on the box body.

[0029] Beneficial effect: The heat dissipation holes can help dissipate the heat generated by the driving parts and the centrifugal barrel during operation, thereby improving the stability of the operation of various components in the box.

[0030] Furthermore, a liquid level sensor is fixedly connected to the concentration tank, and the controller is used to receive liquid level data monitored by the liquid level sensor.

[0031] Beneficial effects: Through the liquid level sensor, staff can understand the concentration effect of duck virus samples in the concentration box, thereby improving the safety of duck virus sample concentration.

[0032] Additional aspects and advantages of the present invention will be set forth in part in the description which follows and, in part, will be obvious from the description which follows, or may be learned by practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 This is an axonometric diagram of a device for filtering, adsorbing and concentrating duck virus samples according to the present invention.

[0034] Figure 2 This is an internal axonometric diagram of a device for filtering, adsorbing and concentrating duck virus samples according to the present invention.

[0035] Figure 3 This is a front cross-sectional view of a device for filtering, adsorbing and concentrating duck virus samples according to the present invention.

[0036] The figure marks in the drawings of the specification include: 1. box body; 2. feed port; 3. connecting plate; 4. centrifugal barrel; 5. rotating rod; 6. threaded sleeve; 7. gear; 8. driving motor; 9. piston cylinder; 10. push rod; 11. fixed wheel; 12. filter plate; 13. elastic ball; 14. baffle; 15. piston head; 16. concentration box; 17. electromagnet. DETAILED DESCRIPTION

[0037] The following is further described in detail through specific implementation methods:

[0038] Example 1:

[0039] As attached Figure 1-Figure 3As shown: A device for filtering, adsorbing and concentrating duck virus samples, including a box body 1, a feed inlet 2 is opened on the top of the box body 1, and a discharge port and a through hole are opened at the bottom of the box body 1. The box body 1 is provided with a filtering component for filtering duck virus samples, a centrifugal component for centrifuging duck virus samples, an auxiliary component for improving the filtering effect, a concentrating component for concentrating duck virus samples, an exhaust component for exhausting the concentration component, and a driving component for driving the centrifugal component, the auxiliary component and the exhaust component.

[0040] like Figure 2 As shown, the drive assembly includes a controller and a drive member. In this embodiment, the drive member is a drive motor 8. The controller is used to control the operation of the drive motor 8. The drive motor 8 is bolted fixedly connected to the housing 1. The output shaft of the drive motor 8 is coaxially bolted and fixedly connected to the rotating rod 5. The rotating rod 5 is bolted fixedly connected to a threaded sleeve 6, which meshes with a gear 7. A fixed rod is screwed fixedly connected to one side of the gear 7. The end of the fixed rod away from the gear 7 is rotatably engaged with the inner wall of the housing 1.

[0041] A baffle 14 is bolted to the interior of the housing 1. An electromagnet 17 is rotatably engaged within the baffle 14. A controller controls the opening and closing of the electromagnet 17. The end of the rotating rod 5, away from the drive motor 8, passes through the baffle 14 and is screwed to the electromagnet 17. The rotating rod 5 rotatably engages with the baffle 14. The electromagnet 17 is magnetically connected to the centrifugal assembly.

[0042] like Figure 1 As shown, the filter assembly includes a filter plate 12, which is bolted securely to the housing 1. The centrifuge assembly includes a centrifuge bucket 4, which is rotatably connected to a baffle 14 and positioned below the filter plate 12. The bottom of the centrifuge bucket 4 is magnetically connected to an electromagnet 17. A centrifugal opening is defined at the bottom of the centrifuge bucket 4, which is screwed to a delivery pipe. The end of the delivery pipe, distal to the centrifugal opening, is screwed securely to the concentrator assembly.

[0043] The auxiliary component includes a fixed wheel 11 fixedly connected to the gear 7 by an eccentric screw, and a connecting plate 3 is rotatably arranged on the outer surface of the fixed wheel 11. An opening for the connecting plate 3 to move is opened on the baffle 14. The connecting plate 3 passes through the opening and slides with the opening. The end of the connecting plate 3 away from the fixed wheel 11 is fixedly connected to an elastic ball 13 by a screw, and the elastic ball 13 is in contact with the filter plate 12.

[0044] The vacuum assembly includes a push rod 10, which is hingedly connected to a fixed wheel 11. A piston cylinder 9 is bolted to the bottom wall of the housing 1. The piston cylinder 9 has an air inlet and an air outlet. Both the inlet and outlet are screwed to a one-way valve. The inlet is screwed to a vacuum line, and the end of the line away from the inlet is screwed to the concentrator assembly. The outlet is connected to a through hole. A piston head 15 is slidably fitted within the piston cylinder 9, and the end away from the inlet is hingedly connected to the push rod 10.

[0045] The concentrator assembly includes a concentrator tank 16, which is bolted to the housing 1. The delivery and exhaust pipes are connected to the concentrator tank 16. A liquid outlet is located at the bottom of the concentrator tank 16, which is connected to the discharge port. A solenoid valve is screwed into the discharge port, and a controller is used to control the opening and closing of the solenoid valve.

[0046] The specific implementation process is as follows: first, the staff can add the duck virus sample into the box 1 from the feed port 2 at the top of the box 1 . After entering the box 1 , the duck virus sample will be filtered through the filter plate 12 .

[0047] At the same time, Figure 2 For example, a worker can use a controller to activate the drive motor 8 and deactivate the electromagnet 17. When the drive motor 8 is activated, it drives the rotating rod 5 to rotate, which in turn drives the threaded sleeve 6 to rotate. The threaded sleeve 6 meshes with the gear 7, which in turn drives the gear 7 to rotate. Since the electromagnet 17 is deactivated, the rotation of the rotating rod 5 does not drive the centrifuge bucket 4 to rotate.

[0048] As the gear 7 rotates, the gear 7 will drive the fixed wheel 11 to rotate. The rotation of the fixed wheel 11 will continuously drive the connecting plate 3 to move up and down, thereby driving the elastic ball 13 to continuously impact the filter plate 12. The impact of the elastic ball 13 prevents the filter plate 12 from being blocked by impurities, and at the same time enhances the filtering effect, so that the duck virus sample can pass through the filter plate 12 more smoothly and enter the centrifuge bucket 4.

[0049] by Figure 3 For example, when the duck virus sample is filtered, the staff can control the electromagnet 17 to start through the controller. After the electromagnet 17 is started, it will adsorb the centrifuge bucket 4 through magnetic force, so that the centrifuge bucket 4 can rotate together with the rotation of the rotating rod 5. The high-speed centrifugal force of the centrifuge bucket 4 is used to separate the components according to the density difference. After centrifugation, the duck virus sample can enter the concentration box 16 through the conveying pipe.

[0050] As the fixed wheel 11 rotates, it drives the push rod 10 to reciprocate, pushing the piston head 15 to slide back and forth within the piston barrel 9. When the piston head 15 slides upward, negative pressure is formed within the piston barrel 9, and air is extracted from the concentration tank 16 through the exhaust pipe and the air inlet, reducing the pressure within the concentration tank 16. When the pressure decreases, the boiling point of the liquid decreases, causing the water in the liquid to evaporate, achieving sample concentration. When concentration is completed, the controller controls the solenoid valve in the discharge port to open, and the concentrated sample flows out of the liquid outlet at the bottom of the concentration tank 16 and is discharged from the box body 1 through the discharge port.

[0051] By evacuating the concentration box 16 through the exhaust pipe and the air inlet, the air pressure in the concentration box 16 is lower than the air pressure in the delivery pipe. At this time, the duck virus sample in the delivery pipe will be adsorbed into the concentration box 16 under the action of the gas pressure, thereby reducing the adhesion of the duck virus sample in the delivery pipe, thereby improving the utilization rate of the duck virus sample.

[0052] Example 2:

[0053] like Figure 3 As shown, the difference from the above embodiment is that the filter plate 12 is inclined.

[0054] The specific implementation process is as follows: by tilting the filter plate 12 , the duck virus sample can be naturally filtered downward under the action of gravity, thereby improving the filtering efficiency of the filter plate 12 .

[0055] Example 3:

[0056] The difference from the above embodiment is that a transparent window is fixedly connected to the box body 1 by screws.

[0057] The specific implementation process is as follows: Through the transparent window, the staff can observe the operation status of different components in the box 1 in real time, thereby improving the safety and accuracy of the staff's operation.

[0058] Example 4:

[0059] The difference from the above embodiment is that a plurality of heat dissipation holes are opened on the box body 1.

[0060] The specific implementation process is as follows: Through the heat dissipation holes, the heat generated by the components in the box body 1 during operation can be better dissipated, thereby increasing the service life of the components in the box body 1.

[0061] Example 5:

[0062] The difference from the above embodiment is that a liquid level sensor is fixedly connected to the concentration tank 16 by screws, and the controller is used to receive liquid level data monitored by the liquid level sensor.

[0063] The specific implementation process is as follows: Through the liquid level sensor, the staff can monitor the liquid level height in the concentration tank 16 in real time. This is helpful for the staff to master the concentration process of the duck virus sample in the concentration tank 16 and improve the safety of the duck virus sample concentration process.

[0064] Obviously, the above embodiments are merely examples for clarity of explanation and are not intended to limit the implementation methods. Those skilled in the art will readily appreciate that other variations or modifications based on the above descriptions are possible. It is not necessary and impossible to enumerate all implementation methods here. Obvious variations or modifications arising therefrom remain within the scope of protection of the present invention.

Claims

1. A device for filtering, adsorbing and concentrating duck virus samples, comprising a housing (1), a top of the housing (1) having an inlet (2), a bottom of the housing (1) having an outlet and a through hole, characterized in that: The box (1) is provided with a filtering component for filtering duck virus samples, a centrifugal component for centrifuging duck virus samples, an auxiliary component for improving filtering effect, a concentration component for concentrating duck virus samples, an exhaust component for exhausting air from the concentration component, and a driving component for driving the centrifugal component, the auxiliary component, and the exhaust component; The driving assembly includes a controller and a driving member, the controller is used to control the operation of the driving member, the driving member is fixedly connected to the box body (1), and the output shaft of the driving member is coaxially fixedly connected to a rotating rod (5); a threaded sleeve (6) is fixedly connected to the rotating rod (5), and the threaded sleeve (6) is meshed with a gear (7); a fixed rod is fixedly connected to one side of the gear (7), and an end of the fixed rod away from the gear (7) is rotatably engaged with the inner wall of the box body (1); A baffle (14) is fixedly connected to the box body (1), an electromagnet (17) is rotatably engaged in the baffle (14), a controller is used to control the opening and closing of the electromagnet (17), an end of the rotating rod (5) away from the driving member passes through the baffle (14) and is fixedly connected to the electromagnet (17), and the rotating rod (5) is rotatably engaged with the baffle (14); The electromagnet (17) is magnetically connected to the centrifugal assembly.

2. The duck virus sample filtration, adsorption and concentration device according to claim 1, characterized in that: The filter assembly comprises a filter plate (12), and the filter plate (12) is fixedly connected inside the box (1).

3. The duck virus sample filtration, adsorption and concentration device according to claim 2, characterized in that: The centrifugal assembly includes a centrifugal bucket (4), the centrifugal bucket (4) is rotatably connected to the baffle (14), the centrifugal bucket (4) is located below the filter plate (12), and the bottom of the centrifugal bucket (4) is magnetically connected to the electromagnet (17); A centrifugal port is formed at the bottom of the centrifugal barrel (4), and the centrifugal port is fixedly connected to a delivery pipe, and one end of the delivery pipe away from the centrifugal port is fixedly connected to the concentration component.

4. The duck virus sample filtration, adsorption and concentration device according to claim 3, characterized in that: The auxiliary component comprises a fixed wheel (11) eccentrically fixedly connected to the gear (7); a connecting plate (3) is rotatably arranged on the outer surface of the fixed wheel (11); an opening for the connecting plate (3) to move is opened on the baffle (14); the connecting plate (3) passes through the opening and slides with the opening; an elastic ball (13) is fixedly connected to one end of the connecting plate (3) away from the fixed wheel (11); and the elastic ball (13) contacts the filter plate (12).

5. The duck virus sample filtration, adsorption and concentration device according to claim 4, characterized in that: The air extraction assembly comprises a push rod (10), which is hinged to a fixed wheel (11); The bottom wall of the box body (1) is fixedly connected to a piston cylinder (9), and an air inlet and an air outlet are formed on the piston cylinder (9). The air inlet and the air outlet are both fixedly connected to a one-way valve. The air inlet is fixedly connected to an exhaust pipe, and the end of the exhaust pipe away from the air inlet is fixedly connected to the concentration component; the air outlet is connected to the through hole; A piston head (15) is slidably fitted in the piston cylinder (9), and one end of the piston head (15) away from the air inlet is hinged to the push rod (10).

6. The duck virus sample filtration, adsorption and concentration device according to claim 5, characterized in that: The concentration assembly includes a concentration box (16), the concentration box (16) is fixedly connected to the box body (1), and the delivery pipeline and the exhaust pipeline are respectively connected to the concentration box (16); A liquid outlet is provided at the bottom of the concentration box (16), which is communicated with the material outlet. A solenoid valve is fixedly connected in the material outlet, and a controller is used to control the opening and closing of the solenoid valve.

7. The duck virus sample filtration, adsorption and concentration device according to claim 6, characterized in that: The filter plate (12) is inclined.

8. The device for filtering, adsorbing and concentrating duck virus samples according to claim 7, characterized in that: A transparent window is fixedly connected to the box body (1).

9. The device for filtering, adsorbing and concentrating duck virus samples according to claim 8, characterized in that: The box body (1) is provided with a plurality of heat dissipation holes.

10. The device for filtering, adsorbing and concentrating duck virus samples according to claim 9, characterized in that: A liquid level sensor is fixedly connected to the concentration tank (16), and the controller is used to receive liquid level data monitored by the liquid level sensor.

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