Immune cell culture bottle

Abstract

The invention relates to the technical field of cell culture devices, and particularly discloses an immune cell culture bottle which comprises a bottle body, a material changing assembly is arranged in an inner cavity of the bottle body, a limiting assembly is arranged at the bottom end of the bottle body, the material changing assembly comprises a top cover, a first connecting block is fixedly installed on the outer surface of the top cover, and a discharging mechanism is rotationally connected to the inner cavity of the top cover. A regulating mechanism is arranged in an inner cavity of the top cover, a sliding groove is formed in the inner cavity of the top cover, a culture medium in the culture bottle can be replaced through the material changing assembly under the condition that the bottle cover is not opened, immune cells in the culture bottle are prevented from being exchanged with outside air in a large amount, and the stability of the temperature in the culture bottle is kept; according to the culture box, a proper growth condition is created for cells, quantitative feeding can be conducted according to needs when a culture medium is added, the culture bottle can be fixed to the inner wall of the culture box through the limiting assembly, and the stability of the culture bottle in the culture box is improved.

Description

Technical Field

[0001] The present application relates to the field of cell culture devices, and in particular to an immune cell culture flask. Background Art

[0002] Immune cells are the core components of the immune system. By culturing immune cells in vitro, researchers can deeply observe their behavior and functional changes under different conditions. As a core link in modern biomedical research, immune cell culture plays a key role in tumor immunotherapy, vaccine development and regenerative medicine. Immune cell culture requires culture flasks, which are mainly based on the key role of culture flasks in maintaining a sterile environment, providing physical support, controlling culture conditions, facilitating operation and observation, and meeting the needs of large-scale culture.

[0003] The existing technology still has the following problems:

[0004] 1. When culturing immune cells in culture flasks, the culture medium needs to be replaced regularly. Existing technologies require opening the bottle cap to replace the culture medium. When pouring and adding culture medium, the bottle mouth exchanges a large amount of air with the outside air for a long time. Opening the culture bottle cap will cause the temperature inside the culture bottle to fluctuate due to the influence of the external environment. External microorganisms will also enter the culture bottle, compete with the cells for nutrients, and may secrete harmful substances, resulting in cell growth inhibition or even death. In addition, since there are strict requirements on the amount of culture medium each time the culture medium is added, this greatly increases the exposure time of the culture bottle, further affecting cell growth and survival.

[0005] 2. A constant temperature environment is required for culturing immune cells. Culture flasks are usually placed in an incubator to maintain a constant temperature environment. Existing culture flasks do not have a limiting mechanism. If there is no limiting mechanism to fix the culture flasks, the culture flasks may be displaced, tipped over, or even broken due to vibration, movement, or careless human operation of the box. Culture flasks without a limiting mechanism may not be able to be arranged neatly in the constant temperature box, resulting in a waste of space inside the incubator. Summary of the Invention

[0006] In order to overcome the problem that the bottle mouth exchanges a large amount of air with the outside air for a long time when pouring and adding culture medium, opening the culture bottle lid will cause the temperature inside the culture bottle to fluctuate due to the influence of the external environment. External microorganisms will also enter the culture bottle, compete with cells for nutrients, and may secrete harmful substances, resulting in cell growth inhibition or even death. In addition, since there are strict requirements on the amount of culture medium each time culture medium is added, this greatly increases the exposure time of the culture bottle, further affecting the growth and survival of cells. The culture bottle does not have a limiting mechanism. If there is no limiting mechanism to fix the culture bottle, the culture bottle may be displaced, dumped or even broken due to vibration, movement or careless human operation of the box. Culture bottles without a limiting mechanism may not be able to be arranged neatly in the constant temperature box, resulting in waste of internal space of the incubator and other shortcomings. The purpose of the present invention is to provide an immune cell culture bottle to solve the above-mentioned shortcomings.

[0007] The present application provides an immune cell culture bottle, comprising a bottle body, a material changing assembly is provided in the inner cavity of the bottle body, a limiting assembly is provided at the bottom end of the bottle body, the material changing assembly comprises a top cover, a first connecting block is fixedly installed on the outer surface of the top cover, the inner cavity of the top cover is rotatably connected to a material discharge mechanism, the inner cavity of the top cover is provided with an adjustment mechanism, a sliding groove is provided in the inner cavity of the top cover, the inner cavity of the top cover is slidably connected to a sliding disk, the lower surface of the sliding disk is in close contact with an elastic mechanism, a floating mechanism is provided below the sliding disk, a discharge mechanism is provided in the middle part of the top cover, a material discharge groove is provided on the outer surface of the sliding disk, and a second fixed block is fixedly installed on the upper surface of the top cover.

[0008] Furthermore, the top cover and the inner wall of the bottle body are connected by threads, the side of the upper surface of the top cover is hollowed out, and the upper surface of the top cover and the side wall are fixedly connected by a first connecting block. A connecting hole is opened on the upper surface of the top cover, the sliding plate and the sliding groove are slidably connected, and the discharge trough and the connecting hole are staggered.

[0009] Furthermore, the unloading mechanism includes a rotating disk, a first connecting rod is fixedly installed at both ends of the lower surface of the rotating disk, the lower surface of the first connecting rod is fixedly connected to the connecting disk, the lower surface of the connecting disk is fixedly connected to the first barrel, the inner cavity of the first barrel is slidably connected to the second barrel, the bottom end of the second barrel is fixedly connected to the lifting disk, both ends of the first barrel and the second barrel are hollow, the rotating disk and the top cover are rotatably connected, the first connecting rod is located at the hollowed-out side of the top cover, the connecting holes on the upper surface of the first barrel and the top cover are aligned, the upper surface of the connecting disk is in close contact with the top bottom wall of the top cover, the lower surface of the lifting disk is in close contact with the upper surface of the sliding disk, and there is a pointer on the inner wall of the rotating disk.

[0010] Furthermore, the adjusting mechanism includes a lifting rod, which is movably connected to the inner cavity of the top cover, and the upper end of the lifting rod is evenly provided with a limiting groove, and the upper surface of the top cover is fixedly installed with a first fixed block, and the inner cavity of the first fixed block is slidably connected with a clamping block, and the end of the clamping block away from the first fixed block is engaged with the inner cavity of the limiting groove, and the lifting rod and the clamping block are rotatably connected, and the upper surface of the clamping block is fixedly installed with a first sliding rod, and the first sliding rod and the first fixed block are slidably connected, and the lower end of the lifting rod is fixedly connected with an adjusting sleeve, and the adjusting sleeve is fixedly connected to the second barrel, and the upper surface of the lifting disk and the lower surface of the adjusting sleeve are tightly fitted.

[0011] Furthermore, the elastic mechanism includes a connecting frame, a fixing rod is fixedly installed on the outer surface of the connecting frame, the outer surface of the fixing rod is slidably connected to the first slide, the middle part of the fixing rod is sleeved with a first spring, both ends of the first spring are in contact with the first slide, the inner cavity of the first slide is rotatably connected to a connecting strip, the end of the connecting strip away from the first slide is rotatably connected to a support sleeve, the support sleeve is sleeved on the outer surface of the lifting rod, and the upper surface of the support sleeve is tightly fitted with the lower surface of the sliding disk.

[0012] Furthermore, the floating mechanism includes a fixed frame, a first protrusion is fixedly installed on the top bottom wall of the fixed frame, the two ends of the middle protrusion of the first protrusion are flush with the top bottom wall of the first protrusion, a second connecting rod is fixedly installed on the lower surface of the sliding plate, the second connecting rod is located on the side away from the discharge chute, the bottom end of the second connecting rod is fixedly connected to a fixed bar, the inner cavity of the fixed bar is rollingly connected to a rolling ball, the fixed frame and the bottom end of the lifting rod are fixedly connected, the bottom end of the connecting frame and the outer surface of the fixed frame are fixedly connected, the support sleeve and the sliding plate are rotatably connected, the fixed bar and the support sleeve do not contact, the top of the rolling ball and the top bottom wall of the fixed frame are at the same height, and there is a gap between the fixed frame and the inner wall of the top cover.

[0013] The top end face of said sliding arm is fixedly provided with a toothed connecting strip which is cooperatively connected with said toothed connecting strip.

[0014] Furthermore, the limiting assembly includes a gear, which is rotatably connected to the discharge pipe. A socket is provided on the outer surface of the gear. The bottom end of the discharge pipe is rotatably connected to a bolt through a thread, and the bolt is engaged with the socket. Racks are provided on both sides of the gear, and the racks are meshed with the gear. One side of the gear is fixedly connected to a limiting rod, and the limiting rod is slidably connected to the bottom wall of the bottle body. One end of the rack is fixedly connected to the limiting mechanism.

[0015] Furthermore, the limiting mechanism includes an adjusting seat, the inner cavity of the adjusting seat is provided with a third spring, the inner cavity of the adjusting seat is slidably connected to the limiting strip, the outer surface of the limiting strip is fixedly connected to the elastic seat, the inner cavity of the elastic seat is slidably connected to the second slide, the outer surface of the second slide is sleeved with a fourth spring, the end of the second slide away from the fourth spring is rotatably connected to the pressure wheel, the inner cavity of the elastic seat is rotatably connected to the balancing seat, and the outer surface of the balancing seat is fixedly connected to the clamping block.

[0016] Furthermore, the adjustment seat and the rack are fixedly connected, the third spring is located between the inner wall of the adjustment seat and the limit strip, the fourth spring is located on the inner wall diameter of the second slide seat and the elastic seat, and the balance seat is symmetrically distributed about the pressure wheel.

[0017] The technical solution provided by this application has at least the following technical effects or advantages:

[0018] 1. The use of a material changing component effectively solves the problem that the existing technology requires opening the bottle cap to change the culture medium. When pouring and adding culture medium, the bottle mouth exchanges a large amount of air with the outside air for a long time. Opening the culture bottle cap will cause the temperature in the culture bottle to fluctuate due to the influence of the external environment. External microorganisms will also enter the culture bottle, compete with the cells for nutrients, and may secrete harmful substances, resulting in cell growth inhibition or even death. In addition, since there is a strict requirement for the amount of culture medium each time the culture medium is added, this greatly increases the exposure time of the culture bottle, further affecting the growth and survival of the cells. The present invention can The culture medium in the culture bottle can be replaced without opening the bottle cap to avoid large-scale exchange of immune cells in the culture bottle with the outside air, maintain the stability of the internal temperature of the culture bottle, and create a suitable growth condition for the cells. When adding culture medium, quantitative feeding can be carried out according to demand. Quantitative feeding can achieve precise control of the culture medium, ensuring that the amount or proportion of each feeding meets the preset standards. At the same time, the capacity of quantitative feeding can be adjusted to improve the fault tolerance of feeding. In addition, when feeding, the culture medium can be prevented from adhering to the inner wall of the container, thereby improving the accuracy of quantitative feeding and facilitating better growth of immune cells.

[0019] 2. The use of a limiting component effectively solves the need for a constant temperature environment when culturing immune cells. Culture bottles are usually placed in an incubator to maintain a constant temperature environment. Existing culture bottles do not have a limiting mechanism. If there is no limiting mechanism to fix the culture bottles, the culture bottles may be displaced, tipped over, or even broken due to vibration, movement, or careless human operation of the box. Culture bottles without a limiting mechanism may not be able to be arranged neatly in the constant temperature box, resulting in a waste of space inside the incubator. The present invention can fix the culture bottles to the inner wall of the incubator through the limiting component, thereby improving the stability of the culture bottles inside the incubator, preventing the culture bottles from tipping over and damaging the culture bottles and the incubator, and at the same time improving the utilization efficiency of the incubator. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a schematic diagram of the overall structure of the embodiment of the present application;

[0021] Figure 2 Schematic diagram of the cross-section of the bottle structure in the embodiment of the present application;

[0022] Figure 3 Schematic cross-sectional view of the top cover structure in the embodiment of the present application;

[0023] Figure 4 Schematic diagram of the blanking mechanism structure in the embodiment of the present application;

[0024] Figure 5 Schematic diagram of the structure of the adjustment mechanism in the embodiment of the present application;

[0025] Figure 6 Schematic diagram of the elastic mechanism structure in the embodiment of the present application;

[0026] Figure 7 Schematic diagram of the floating mechanism structure in an embodiment of the present application;

[0027] Figure 8 This is a schematic diagram of the connection of the sliding disk structure in an embodiment of the present application;

[0028] Figure 9 Schematic diagram of the partial structure of the discharge mechanism in the embodiment of the present application;

[0029] Figure 10 Schematic diagram of the cross-section of the discharge pipe structure in the embodiment of the present application;

[0030] Figure 11 Schematic diagram of the structure of the limiting component in the embodiment of the present application;

[0031] Figure 12 Schematic diagram of the cross-section of the limiting mechanism structure in the embodiment of the present application.

[0032] In the figure: 1. bottle body; 2. material changing assembly; 21. top cover; 22. first connecting block; 23. unloading mechanism; 231. rotating disk; 232. first connecting rod; 233. connecting disk; 234. first barrel; 235. second barrel; 236. lifting disk; 24. adjusting mechanism; 241. lifting rod; 242. limiting groove; 243. first fixing block; 244. clamping block; 245. first slide bar; 246. adjusting sleeve; 25. slide groove; 26. slide plate; 27. elastic mechanism; 271. connecting frame; 272. fixing rod; 273. first slide seat; 274. first spring; 275. connecting strip; 276. supporting sleeve; 28. floating mechanism; 281. fixing frame; 282. first protrusion; 283. second connecting rod; 2 84. Fixed bar; 285. Ball; 29. Discharge mechanism; 291. Discharge pipe; 292. Fixed ring; 293. Second slide bar; 294. Second spring; 295. Discharge barrel; 296. Discharge hole; 297. Lifting ring; 298. Second protrusion; 299. Rotating rod; 2910. Second connecting block; 2911. Extrusion rod; 210. Discharge chute; 211. Second fixed block; 3. Limiting assembly; 31. Gear; 32. Socket; 33. Bolt; 34. Rack; 35. Limiting rod; 36. Limiting mechanism; 361. Adjusting seat; 362. Third spring; 363. Limiting bar; 364. Elastic seat; 365. Second slide seat; 366. Fourth spring; 367. Pressure wheel; 368. Balance seat; 369. Clamp. DETAILED DESCRIPTION

[0033] When pouring and adding culture medium, the bottle mouth exchanges a large amount of air with the outside air for a long time. The present invention can replace the culture medium in the culture bottle without opening the bottle cap through the material replacement component, thereby avoiding a large amount of exchange between the immune cells in the culture bottle and the outside air, maintaining the stability of the internal temperature of the culture bottle, and creating a suitable growth condition for the cells; if the culture bottle does not have a limiting mechanism, the present invention can fix the culture bottle to the inner wall of the incubator through the limiting component, thereby improving the stability of the culture bottle inside the incubator.

[0034] In order to better understand the above technical solution, the above technical solution will be described in detail below with reference to the accompanying drawings and specific implementation methods.

[0035] See also Figure 1 As shown, an immune cell culture bottle includes a bottle body 1, the inner cavity of the bottle body 1 is provided with a material changing component 2, and the bottom end of the bottle body 1 is provided with a limiting component 3. The culture medium in the bottle body 1 is replaced by the material changing component 2, that is, the culture medium in the bottle body 1 is taken out and the culture medium is added in a quantitative manner. When the bottle body 1 is cultured, the bottle body 1 needs to be placed in an incubator. The bottle body 1 can be fixed inside the incubator by the limiting component 3 to improve the stability of the bottle body 1.

[0036] See also Figure 2 、 Figure 3 and Figure 7 As shown, the material changing assembly 2 includes a top cover 21, the outer surface of the top cover 21 is fixedly mounted with a first connecting block 22, the inner cavity of the top cover 21 is rotatably connected to the discharge mechanism 23, the inner cavity of the top cover 21 is provided with an adjusting mechanism 24, the inner cavity of the top cover 21 is provided with a slide groove 25, the inner cavity of the top cover 21 is slidably connected to a slide plate 26, the lower surface of the slide plate 26 is in close contact with an elastic mechanism 27, a floating mechanism 28 is provided below the slide plate 26, a discharge mechanism 29 is provided in the middle part of the top cover 21, the outer surface of the slide plate 26 is provided with a discharge chute 210, the upper surface of the top cover 21 is fixedly mounted with a second fixed block 211, the second fixed block 211 is used to limit the discharge mechanism 29, the top cover 21 and the inner wall of the bottle body 1 are connected by threads, the upper surface side of the top cover 21 is hollowed out, and the upper surface and side wall of the top cover 21 are fixedly connected by the first connecting block 22, the top cover 21 A connecting hole is provided on the upper surface, and the sliding plate 26 is slidably connected to the slide groove 25, and the discharge groove 210 is staggered with the connecting hole. When replacing the culture medium in the inner cavity of the bottle body 1, the culture medium in the bottle body 1 is discharged through the discharge mechanism 29, and then the culture medium is quantitatively added through the discharge mechanism 23. The discharge mechanism 23 can be rotated in the inner cavity of the top cover 21 to make the culture medium in the inner cavity of the discharge mechanism 23 fall from the discharge groove 210 into the inner cavity of the bottle body 1. During the rotation process, the floating mechanism 28 can drive the discharge mechanism 23 to shake, so that the culture medium in the inner cavity of the discharge mechanism 23 falls as much as possible, thereby achieving the effect of improving the precise dosage. The amount of the discharge mechanism 23 can be adjusted by the adjustment mechanism 24, so that the sliding plate 26 slides in the inner cavity of the slide groove 25, and the elastic mechanism 27 is used to support the sliding plate 26 so that the bottom end of the sliding plate 26 and the discharge mechanism 23 always maintain close contact to prevent the culture medium from leaking sideways.

[0037] See also Figure 4 、 Figure 5 and Figure 7As shown, the unloading mechanism 23 includes a rotating disk 231, and first connecting rods 232 are fixedly installed at both ends of the lower surface of the rotating disk 231, and the lower surface of the first connecting rod 232 is fixedly connected to a connecting disk 233, and the lower surface of the connecting disk 233 is fixedly connected to a first barrel 234, and the inner cavity of the first barrel 234 is slidably connected to the second barrel 235, and the bottom end of the second barrel 235 is fixedly connected to a lifting disk 236, and both ends of the first barrel 234 and the second barrel 235 are hollow, and the rotating disk 231 is rotatably connected to the top cover 21, and the first connecting rod 232 is located at the hollowed-out part of the side of the top cover 21, and the connecting holes on the upper surface of the first barrel 234 and the top cover 21 are aligned, and the upper surface of the connecting disk 233 is in close contact with the top bottom wall of the top cover 21, and the lower surface of the lifting disk 236 is in close contact with the top bottom wall of the top cover 21. The surface is in close contact with the upper surface of the sliding plate 26. There is a pointer on the inner wall of the rotating disk 231, which is convenient for understanding the overall rotation position of the unloading mechanism 23. When the pointer points to one of the first connecting blocks 22, material can be added. When material is not added, the pointer points to the other first connecting block 22, ensuring that the top cover 21 blocks the upper surface of the rotating disk 231 to prevent the exchange of external air and the bottle body 1. The adjusting mechanism 24 includes a lifting rod 241, which is movably connected to the inner cavity of the top cover 21, that is, the lifting rod 241 can rotate in the inner cavity of the top cover 21 and can also move up and down in the inner cavity of the top cover 21. The upper end of the lifting rod 241 is evenly provided with a limiting groove 242, and a first fixed block 243 is fixedly installed on the upper surface of the top cover 21. The inner cavity of the first fixed block 243 is slidably connected to a card The first and second fixing blocks 243 are connected in a sliding manner to each other, and the first and second fixing blocks 243 are connected in a sliding manner to each other. The lower end of the lifting rod 241 is fixedly connected with an adjusting sleeve 246, and the adjusting sleeve 246 is fixedly connected with the second barrel 235. The upper surface of the lifting plate 236 and the lower surface of the adjusting sleeve 246 fit tightly together. When the culture medium is quantitatively added to the inside of the bottle 1, the funnel or hopper is aligned with the communicating hole on the upper surface of the top cover 21, and the culture medium is poured into the funnel or hopper. At this time, the first barrel 234 and the communicating hole are aligned, so that the inner cavities of the first barrel 234 and the second barrel 235 are After the culture medium is filled, the first connecting rod 232 drives the connecting disk 233 to rotate in the inner cavity of the top cover 21 by rotating the rotating disk 231. The rotation of the connecting disk 233 drives the first barrel 234 to rotate. The rotation of the first barrel 234 drives the second barrel 235 to rotate. The rotation of the second barrel 235 drives the lifting disk 236 to rotate. The rotation of the lifting disk 236 drives the second barrel 235 to pass through the discharge chute 210. At this time, the culture medium in the inner cavities of the first barrel 234 and the second barrel 235 falls into the inner cavity of the bottle body 1 at the discharge chute 210. The capacity of the first barrel 234 and the second barrel 235 is fixed. By continuously rotating the rotating disk 231, the inner cavity of the bottle body 1 can be quantitatively added each time. When the first barrel 234 and the connecting hole are aligned, it is necessary to ensure that they stay for a certain period of time.To ensure that the culture medium can fill the inner cavities of the first barrel 234 and the second barrel 235, when the capacity of the first barrel 234 and the second barrel 235 needs to be adjusted, the first sliding rod 245 is slid to drive the block 244 to be stored in the inner cavity of the first fixed block 243. At this time, the block 244 and the limiting groove 242 are disengaged, and the position of the lifting rod 241 in the inner cavity of the top cover 21 is moved. When the appropriate position is reached, the block 244 is engaged with the limiting groove 242 again, so that the height of the lifting rod 241 in the inner cavity of the top cover 21 remains stable. When the lifting rod 241 moves, it drives the adjustment sleeve 246 to move, and the movement of the adjustment sleeve 246 drives the second barrel 235 to move. At this time, the position of the second barrel 235 in the inner cavity of the first barrel 234 changes, that is, the capacity of the first barrel 234 and the second barrel 235 changes. When the lifting rod 241 moves, it drives the floating mechanism 28 to move. The movement of the floating mechanism 28 drives the elastic mechanism 27 to move. The elastic mechanism 27 generates an extrusion force on the sliding plate 26 so that the upper surface of the sliding plate 26 is always in contact with the lower surface of the lifting plate 236. That is, the movement of the lifting rod 241 drives the sliding plate 26 to slide in the inner cavity of the slide groove 25, so that the capacity of the first barrel 234 and the second barrel 235 can be adjusted according to demand. Quantitative dispensing can achieve precise control of the culture medium, ensuring that the amount or ratio of each dispensing meets the preset standard, creating a suitable growth condition for immune cells.

[0038] See also Figure 3 、 Figure 6 、 Figure 7 and Figure 8As shown, the elastic mechanism 27 includes a connecting frame 271, a fixing rod 272 is fixedly installed on the outer surface of the connecting frame 271, and a first slide 273 is slidably connected to the outer surface of the fixing rod 272. A first spring 274 is sleeved on the middle part of the fixing rod 272, and both ends of the first spring 274 are in contact with the first slide 273. The inner cavity of the first slide 273 is rotatably connected to a connecting strip 275, and the end of the connecting strip 275 away from the first slide 273 is rotatably connected to a support sleeve 276. The support sleeve 276 is sleeved on the outer surface of the lifting rod 241, and the upper surface of the support sleeve 276 is tightly fitted with the lower surface of the sliding plate 26. The floating mechanism 28 includes a fixing frame 281, and a first protrusion 282 is fixedly installed on the top and bottom wall of the fixing frame 281. The two ends of the middle protrusion of the first protrusion 282 are flush with the top bottom wall of the first protrusion 282, and the second connecting rod 283 is fixedly installed on the lower surface of the sliding plate 26. The second connecting rod 283 is located on the side away from the discharge chute 210, and the bottom end of the second connecting rod 283 is fixedly connected to the fixing bar 284. The inner cavity of the fixing bar 284 is rollingly connected with the ball 285. The fixing frame 281 is fixedly connected to the bottom end of the lifting rod 241, and the bottom end of the connecting frame 271 is fixedly connected to the outer surface of the fixing frame 281. The support sleeve 276 is rotatably connected to the sliding plate 26. The fixing bar 284 and the support sleeve 276 do not contact. The top of the ball 285 is at the same height as the top bottom wall of the fixing frame 281. There is a gap between the fixing frame 281 and the inner wall of the top cover 21. The elastic mechanism 27 is used to support the sliding plate 26 so that the sliding plate 26 is close to the lower surface of the lifting plate 236, which is convenient for controlling the capacity of the first barrel 234 and the second barrel 235. In addition, the elastic mechanism 27 can cause the second barrel 235 to continuously shake in the inner cavity of the first barrel 234 during the unloading process of the unloading mechanism 23, so as to prevent the culture medium from adhering to the inner walls of the first barrel 234 and the second barrel 235 during unloading, so that the culture medium falls completely each time the material is unloaded. When the rotating disk 231 is rotated, the adjusting sleeve 246 is driven to rotate, the adjusting sleeve 246 rotates to drive the lifting rod 241 to rotate, the lifting rod 241 rotates to drive the fixing frame 281 to rotate, the fixing frame 281 rotates to drive the first protrusion 282 to rotate, and the first protrusion 282 rotates to drive the second protrusion 282 to rotate. A protrusion 282 contacts the ball 285 on the fixing bar 284. At this time, the first protrusion 282 and the ball 285 generate an interaction force. When the second barrel 235 approaches the discharge chute 210, the first protrusion 282 contacts the ball 285. At this time, the sliding plate 26 on the second connecting rod 283 is driven to generate an extrusion force on the support sleeve 276, so that the support sleeve 276 approaches the connecting frame 271. When the support sleeve 276 moves toward the connecting frame 271, it drives the connecting bar 275 to rotate in the inner cavity of the first slide seat 273 to compress the first spring 274. The elastic force of the first spring 274 causes the support sleeve 276 to return to its original position. As a result, when the first protrusion 282 passes the ball 285, the second barrel 235 continuously slides in the inner cavity of the first barrel 234.This allows the culture medium attached to the inner walls of the first and second barrels 234, 235 to be shaken off when the bottom of the second barrel 235 passes through the discharge chute 210. Because the connecting hole at the top of the top cover 21 is misaligned with the discharge chute 210, the first protrusion 282 slowly contacts the ball 285 only when the second barrel 235 passes through the discharge chute 210. This ensures that shaking only occurs when the second barrel 235 passes through the discharge chute 210, facilitating the discharge of the culture medium and improving the accuracy of quantitative discharge.

[0039] See also Figure 8 and Figure 9As shown, the discharge mechanism 29 includes a discharge pipe 291, a fixed ring 292 is fixedly installed on the inner wall of the discharge pipe 291, a second slide rod 293 is fixedly connected to the inner cavity of the fixed ring 292, a second spring 294 is sleeved on the outer surface of the second slide rod 293, a discharge cylinder 295 is fixedly installed on the upper surface of the fixed ring 292, a discharge hole 296 is opened at the upper end of the discharge cylinder 295, a lifting ring 297 is sleeved on the top of the discharge cylinder 295, the lifting ring 297 is slidably connected to the second slide rod 293, the second spring 294 is located between the fixed ring 292 and the lifting ring 297, a second protrusion 298 is fixedly installed on the upper surface of the lifting ring 297, and the middle protrusions of the second protrusion 298 and the lifting holes are connected. The outer surface of the lowering ring 297 is flush, the inner cavity of the second fixed block 211 is rotatably connected to the rotating rod 299, the bottom end of the rotating rod 299 is fixedly connected to the second connecting block 2910, and the two ends of the second connecting block 2910 are fixedly connected to the extrusion rod 2911, the extrusion rod 2911 and the upper surface of the lifting ring 297 are fitted, and the rotating rod 299 rotates to drive the extrusion rod 2911 to pass through the outer surface of the second protrusion 298, the discharge hole 296 is close to the lower surface of the lifting ring 297, the discharge pipe 291 and the bottom of the bottle body 1 are fixedly sleeved, the upper surface of the lifting ring 297 is flush with the bottom wall of the bottle body 1, the rotating rod 299 passes through the inner cavity of the lifting rod 241, and the rotating rod 299 and the inner cavity of the lifting rod 241 There is a gap. When the culture medium in the inner cavity of the bottle body 1 is replaced, the second connecting block 2910 is driven to rotate by rotating the rotating rod 299. The rotation of the second connecting block 2910 drives the squeezing rod 2911 to squeeze the second protrusion 298 on the lifting ring 297. The squeezing rod 2911 is continuously pressed on the second protrusion 298 to make the lifting ring 297 squeeze the second spring 294. At this time, the lifting ring 297 slides on the second sliding rod 293, that is, the lifting ring 297 slides downward in the inner cavity of the discharge pipe 291. The sliding of the lifting ring 297 makes the discharge hole 296 on the discharge cylinder 295 appear inside the bottle body 1, and the culture medium can enter the discharge hole 296 from the bottom end of the bottle body 1. 6, flows out through the bottom end of the fixing ring 292, and when discharging the culture medium, the bottle body 1 can be tilted left and right to facilitate the culture medium inside the bottle body 1 to flow out from the discharge hole 296. When the culture medium inside the bottle body 1 is completely discharged, the culture medium can be added. Under the elastic force of the second spring 294, the lifting ring 297 is reset to block the discharge hole 296 to prevent the culture medium from flowing out. Therefore, when replacing the culture medium inside the bottle body 1, there is no need to open the top cover 21 to avoid contact with the external air, reduce the exchange of a large amount of air inside the bottle body 1 and the outside air, keep the stability of the bottle body 1 stable, and create a suitable growth condition for immune cells.

[0040] See also Figure 2 and Figure 11As shown, the limiting assembly 3 includes a gear 31, the gear 31 and the discharge pipe 291 are rotatably connected, a socket 32 is provided on the outer surface of the gear 31, and the bottom end of the discharge pipe 291 is rotatably connected to a bolt 33 through a thread, the bolt 33 and the socket 32 are plugged in, and racks 34 are provided on both sides of the gear 31, the rack 34 and the gear 31 are meshed, and one side of the gear 31 is fixedly connected to a limiting rod 35, and the limiting rod 35 is slidably connected to the bottom wall of the bottle body 1, and one end of the rack 34 is fixedly connected to a limiting mechanism 36. When the bottle body 1 is placed in the incubator, the spacing of the limiting mechanism 36 is adjusted according to the space inside the incubator. Rotate the bolt 33 to disengage the bolt 33 and the socket 32. At this time, the rotating gear 31 drives the rack 34 to move. The limiting rod 35 is used to limit the rack 34, so that the limiting mechanism 36 moves symmetrically at the bottom end of the bottle body 1. The spacing of the limiting mechanism 36 can be adjusted to the spacing of the bottom grille of the constant temperature box, so that the limiting mechanism 36 can be engaged with the bottom grille of the constant temperature box. If there is no grille at the bottom of the constant temperature box, the limiting mechanism 36 can be adjusted to the spacing between the box body and the door of the constant temperature box, so that when the constant temperature box is closed, the limiting mechanism 36 contacts the box body and the door of the constant temperature box respectively, thereby improving the stability of the bottle body 1 inside the constant temperature box.

[0041] See also Figure 2 and Figure 12As shown, the limiting mechanism 36 includes an adjusting seat 361, an inner cavity of the adjusting seat 361 is provided with a third spring 362, the inner cavity of the adjusting seat 361 is slidably connected to a limiting strip 363, the outer surface of the limiting strip 363 is fixedly connected to an elastic seat 364, the inner cavity of the elastic seat 364 is slidably connected to a second slide 365, the outer surface of the second slide 365 is sleeved with a fourth spring 366, the end of the second slide 365 away from the fourth spring 366 is rotatably connected to a pressure wheel 367, the inner cavity of the elastic seat 364 is rotatably connected to a flat The outer surface of the balance seat 368 is fixedly connected with a clamping block 369, the adjustment seat 361 and the rack 34 are fixedly connected, the third spring 362 is located between the inner wall of the adjustment seat 361 and the limit strip 363, the fourth spring 366 is located between the inner wall diameter of the second slide 365 and the elastic seat 364, the balance seat 368 is symmetrically distributed about the pressure wheel 367, the limit mechanism 36 as a whole has a certain flexibility, which can be adjusted according to the flatness of the inner wall of the constant temperature box. When the clamping block 369 contacts the inner wall of the constant temperature box, the constant temperature box If the interior is uneven, the clamping block 369 will tilt and drive the balance seat 368 to rotate on the elastic seat 364. The rotation of the balance seat 368 drives the pressure wheel 367 to squeeze the second slide 365. At this time, the fourth spring 366 is compressed to facilitate the clamping block 369 to tilt and cooperate. The clamping block 369 can be placed according to the specifications of the interior of the constant temperature box. When the bottle body 1 is placed in the incubator, the distance between the two clamping blocks 369 can be greater than the distance between the inner walls of the constant temperature box, such as the distance between the constant temperature box body and the door, so that when the door is opened, the clamping block 369 can be adjusted. When closed, the clamping block 369 will be squeezed. At this time, the elastic seat 364 slides in the inner cavity of the adjustment seat 361, that is, the limiting strip 363 slides in the inner cavity of the adjustment seat 361 to compress the third spring 362. The elastic force of the third spring 362 increases the squeezing force between the clamping block 369 and the inner wall of the constant temperature box, so that the two clamping blocks 369 can be in close contact with the inner wall of the constant temperature box, so that the bottle body 1 can be stably clamped on the inner wall of the constant temperature box, thereby improving the stability of the bottle body 1 inside the incubator and preventing the bottle body 1 from tipping over and damaging the bottle body 1 and the incubator.

[0042] In summary, the culture medium in the bottle body 1 is replaced by the material replacement component 2, that is, the culture medium in the bottle body 1 is taken out and the culture medium is quantitatively added. When the bottle body 1 is cultured, the bottle body 1 needs to be placed in the incubator. The bottle body 1 can be fixed inside the incubator by the limit component 3 to improve the stability of the bottle body 1. When the culture medium in the inner cavity of the bottle body 1 is replaced, the culture medium in the bottle body 1 is discharged by the discharge mechanism 29, and then the culture medium is quantitatively added by the discharge mechanism 23. The discharge mechanism 23 can be rotated in the inner cavity of the top cover 21 so that the culture medium in the inner cavity of the discharge mechanism 23 falls from the discharge trough 210 into the inner cavity of the bottle body 1. During the rotation, the floating mechanism 28 can drive the discharge mechanism 23 to shake, so that the culture medium in the inner cavity of the discharge mechanism 23 falls as much as possible, thereby achieving the effect of improving the accurate dosage. The amount of the discharge mechanism 23 can be adjusted by the adjustment mechanism 24 so that the slide plate 26 is in the inner cavity of the slide groove 25. The locking mechanism 36 is then engaged with the locking cam 35 and the locking cam 36 is engaged with the locking cam 36 to prevent the locking cam 36 from leaking out of the locking cam 36.

[0043] Obviously, those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if such changes and modifications fall within the scope of the claims and their equivalents, the present invention is intended to include such changes and modifications.

[0044] The above is only a preferred specific implementation method of the embodiment of the present application, but the scope of protection of the present application is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes based on the technical solution and concept of the present application within the technical scope disclosed in the present application, and they should be covered by the scope of protection of the present application.

Claims

1. An immune cell culture bottle, comprising a bottle body (1), characterized in that: The inner cavity of the bottle body (1) is provided with a material changing assembly (2), and the bottom end of the bottle body (1) is provided with a limiting assembly (3); The material changing assembly (2) includes a top cover (21), the outer surface of the top cover (21) is fixedly mounted with a first connecting block (22), the inner cavity of the top cover (21) is rotatably connected with a feeding mechanism (23), the inner cavity of the top cover (21) is provided with an adjusting mechanism (24), the inner cavity of the top cover (21) is provided with a sliding groove (25), the inner cavity of the top cover (21) is slidably connected with a sliding plate (26), the lower surface of the sliding plate (26) is in close contact with an elastic mechanism (27), a floating mechanism (28) is provided below the sliding plate (26), a discharge mechanism (29) is provided at the middle portion of the top cover (21), the outer surface of the sliding plate (26) is provided with a feeding groove (210), and the upper surface of the top cover (21) is fixedly mounted with a second fixing block (211).

2. The immune cell culture flask according to claim 1, characterized in that: The top cover (21) and the inner wall of the bottle body (1) are connected by threads, the side of the upper surface of the top cover (21) is hollowed out, and the upper surface and the side wall of the top cover (21) are fixedly connected by a first connecting block (22), a connecting hole is opened on the upper surface of the top cover (21), the sliding plate (26) and the sliding groove (25) are slidably connected, and the discharge chute (210) and the connecting hole are offset.

3. The immune cell culture flask according to claim 1, characterized in that: The unloading mechanism (23) comprises a rotating disk (231), first connecting rods (232) are fixedly mounted at both ends of the lower surface of the rotating disk (231), the lower surface of the first connecting rod (232) is fixedly connected to a connecting disk (233), the lower surface of the connecting disk (233) is fixedly connected to a first barrel (234), the inner cavity of the first barrel (234) is slidably connected to a second barrel (235), the bottom end of the second barrel (235) is fixedly connected to a lifting disk (236), and the first barrel Both ends of the first barrel (234) and the second barrel (235) are hollow, the rotating disk (231) and the top cover (21) are rotatably connected, the first connecting rod (232) is located at the hollowed-out portion of the side of the top cover (21), the communicating holes on the upper surfaces of the first barrel (234) and the top cover (21) are aligned, the upper surface of the connecting disk (233) is in close contact with the top bottom wall of the top cover (21), the lower surface of the lifting disk (236) is in close contact with the upper surface of the sliding disk (26), and a pointer is provided on the inner wall of the rotating disk (231).

4. The immune cell culture flask according to claim 3, characterized in that: The regulating mechanism (24) includes a lifting rod (241), the lifting rod (241) and the inner cavity of the top cover (21) are movably connected, the upper end of the lifting rod (241) is evenly provided with a limiting groove (242), the upper surface of the top cover (21) is fixedly mounted with a first fixing block (243), the inner cavity of the first fixing block (243) is slidably connected with a clamping block (244), and the end of the clamping block (244) away from the first fixing block (243) and the inner cavity of the limiting groove (242) are slidably connected. The lifting rod (241) and the clamping block (244) are engaged, and the lifting rod (241) and the clamping block (244) are rotatably connected. A first sliding rod (245) is fixedly installed on the upper surface of the clamping block (244). The first sliding rod (245) and the first fixed block (243) are slidably connected. The lower end of the lifting rod (241) is fixedly connected with an adjusting sleeve (246). The adjusting sleeve (246) and the second barrel (235) are fixedly connected. The upper surface of the lifting plate (236) and the lower surface of the adjusting sleeve (246) are tightly fitted.

5. The immune cell culture flask according to claim 1, characterized in that: The elastic mechanism (27) includes a connecting frame (271), a fixing rod (272) is fixedly installed on the outer surface of the connecting frame (271), the outer surface of the fixing rod (272) is slidably connected to a first slide (273), the middle part of the fixing rod (272) is sleeved with a first spring (274), both ends of the first spring (274) are in contact with the first slide (273), the inner cavity of the first slide (273) is rotatably connected to a connecting strip (275), and the end of the connecting strip (275) away from the first slide (273) is rotatably connected to a support sleeve (276), the support sleeve (276) is sleeved with the outer surface of the lifting rod (241), and the upper surface of the support sleeve (276) is tightly fitted with the lower surface of the sliding plate (26).

6. The immune cell culture flask according to claim 5, characterized in that: The floating mechanism (28) includes a fixing frame (281), the top bottom wall of the fixing frame (281) is fixedly mounted with a first protrusion (282), the middle protrusion of the first protrusion (282) is flush with the top bottom wall of the first protrusion (282), the lower surface of the sliding plate (26) is fixedly mounted with a second connecting rod (283), the second connecting rod (283) is located on a side away from the discharge chute (210), the bottom end of the second connecting rod (283) is fixedly connected to a fixing bar (284), the fixing bar The inner cavity of (284) is rollingly connected with a rolling ball (285), the fixing frame (281) and the bottom end of the lifting rod (241) are fixedly connected, the bottom end of the connecting frame (271) and the outer surface of the fixing frame (281) are fixedly connected, the support sleeve (276) and the sliding plate (26) are rotationally connected, the fixing bar (284) and the support sleeve (276) are not in contact, the top end of the rolling ball (285) and the bottom wall of the top end of the fixing frame (281) are at the same height, and there is a gap between the fixing frame (281) and the inner wall of the top cover (21).

7. The immune cell culture flask according to claim 4, characterized in that: The discharging mechanism (29) includes a discharge pipe (291), the inner wall of the discharge pipe (291) is fixedly mounted with a fixing ring (292), the inner cavity of the fixing ring (292) is fixedly connected with a second slide bar (293), the outer surface of the second slide bar (293) is sleeved with a second spring (294), the upper surface of the fixing ring (292) is fixedly mounted with a discharge barrel (295), the upper end of the discharge barrel (295) is provided with a discharge hole (296), the top end of the discharge barrel (295) is sleeved with a lifting ring (297), the lifting ring (297) and the second slide bar (293) are slidably connected, the second spring (294) is located between the fixing ring (292) and the lifting ring (297), the upper surface of the lifting ring (297) is fixedly mounted with a second protrusion (298), the middle protrusion of the second protrusion (298) and the lifting ring (297) are fixedly mounted with two ends. The outer surface of the ring (297) is flush, the inner cavity of the second fixed block (211) is rotatably connected to a rotating rod (299), the bottom end of the rotating rod (299) is fixedly connected to a second connecting block (2910), and both ends of the second connecting block (2910) are fixedly connected to extrusion rods (2911), the extrusion rod (2911) and the upper surface of the lifting ring (297) are in contact, and the rotating rod (299) rotates to drive the extrusion rod (2911) to pass through the outer surface of the second protrusion (298), the discharge hole (296) is close to the lower surface of the lifting ring (297), the discharge pipe (291) and the bottom of the bottle body (1) are fixedly sleeved, the upper surface of the lifting ring (297) and the bottom wall of the bottle body (1) are flush, the rotating rod (299) passes through the inner cavity of the lifting rod (241), and there is a gap between the rotating rod (299) and the inner cavity of the lifting rod (241).

8. The immune cell culture flask according to claim 7, characterized in that: The limiting assembly (3) includes a gear (31), the gear (31) and the discharge pipe (291) are rotatably connected, a socket (32) is provided on the outer surface of the gear (31), the bottom end of the discharge pipe (291) is rotatably connected to a bolt (33) through a thread, the bolt (33) and the socket (32) are engaged, racks (34) are provided on both sides of the gear (31), the racks (34) and the gear (31) are engaged, one side of the gear (31) is fixedly connected to a limiting rod (35), the limiting rod (35) is slidably connected to the bottom wall of the bottle body (1), and one end of the rack (34) is fixedly connected to a limiting mechanism (36).

9. The immune cell culture flask according to claim 8, characterized in that: The limiting mechanism (36) includes an adjusting seat (361), an inner cavity of the adjusting seat (361) is provided with a third spring (362), the inner cavity of the adjusting seat (361) is slidably connected to a limiting strip (363), the outer surface of the limiting strip (363) is fixedly connected to an elastic seat (364), the inner cavity of the elastic seat (364) is slidably connected to a second slide seat (365), the outer surface of the second slide seat (365) is sleeved with a fourth spring (366), the end of the second slide seat (365) away from the fourth spring (366) is rotatably connected to a pressure wheel (367), the inner cavity of the elastic seat (364) is rotatably connected to a balancing seat (368), and the outer surface of the balancing seat (368) is fixedly connected to a clamping block (369).

10. The immune cell culture flask according to claim 9, characterized in that: The adjustment seat (361) and the rack (34) are fixedly connected, the third spring (362) is located between the inner wall of the adjustment seat (361) and the limit strip (363), the fourth spring (366) is located on the inner wall diameter of the second slide seat (365) and the elastic seat (364), and the balancing seat (368) is symmetrically distributed with respect to the pressure wheel (367).

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