High-yield agrocybe cylindracea strain CXG001 as well as culture and cultivation method and application thereof

Abstract

The invention belongs to the technical field of edible mushrooms, and discloses a high-yield agrocybe cylindracea strain CXG001 and a cultivation method and application thereof. The high-yield agrocybe cylindracea strain is a strain CXG001 and is preserved in the China Center for Type Culture Collection, the preservation address is Wuhan University, Wuhan, China, the preservation number is CCTCC NO: M 20251055, and the preservation date is May 14, 2025. Under the same planting condition, the yield is increased by 71.6% compared with that of tea 3, and the yield is increased by 28.1% compared with that of tea 5.

Description

Technical Field

[0001] The invention belongs to the technical field of edible fungi, and in particular relates to a high-yield Agrocybe chaxini strain CXG001, a cultivation method and an application thereof. Background Art

[0002] Agrocybe chaxin Cyclocybe chaxingu , trade name Agrocybe tumefaciens, is one of the main cultivated edible fungi, belonging to the Basidiomycota, Agaricales, Tubariaceae, and Cyclops. Cyclocybe .

[0003] The main cultivated varieties of Agrocybe tumefaciens are still the "Chaxin Mushroom No. 3" and "Chaxin Mushroom No. 5" varieties bred more than a decade ago. These two varieties have degenerated in many bases, and some bases have even stopped producing mushrooms altogether. Therefore, new varieties of Agrocybe tumefaciens with high yield and quality are urgently needed. Summary of the Invention

[0004] In order to overcome the problems existing in the related art, the present invention provides a high-yield Agrocybe chaxini strain CXG001 and a cultivation method and application thereof.

[0005] The technical solution is as follows: a high-yield Agrocybe chaxini strain, the high-yield Agrocybe chaxini strain is strain CXG001, deposited in the China Center for Type Culture Collection, deposit address: Wuhan University, Wuhan, China, deposit number: CCTCC NO: M20251055, deposit date: May 14, 2025, classified and named: Cyclocybe chaxingu CXG001.

[0006] Another object of the present invention is to provide a method for culturing a high-yield Agrocybe chaxini strain, which is used for culturing the high-yield Agrocybe chaxini strain, and the method comprises: S1, remove the roots of wild-type Agrocybe CXG001, remove the soil and impurities on the surface with alcohol cotton, and disinfect; S2, cutting the wild-type Agrocybe CXG001 fruiting body longitudinally to obtain the fruiting body tissue block, placing it in the prepared PDA culture medium, and after mycelium grows, picking the PDA culture medium containing mycelium and culturing it in a new PDA culture dish, repeating the operation multiple times; S3. When the mycelium has grown completely over the culture dish, small cubes of culture medium containing mycelium are cut out, and multiple cubes of culture medium are placed into a cryopreservation tube containing sterile water. The cryopreservation tube is sealed with a cap for storage.

[0007] In step S3, small cubes of culture medium containing mycelia with a side length of 1-2 mm are cut and stored at 4°C.

[0008] Another object of the present invention is to provide a high-yield Agrocybe chaxini strain for use as a parent in high-yield Agrocybe chaxini breeding, and to use the high-yield Agrocybe chaxini strain for breeding.

[0009] Another object of the present invention is to provide a high-yield Agrocybe chaxini mycelium, which is obtained by culturing the high-yield Agrocybe chaxini strain.

[0010] Another object of the present invention is to provide a high-yield Agrocybe chaxini fruiting body, which is obtained by culturing the high-yield Agrocybe chaxini strain.

[0011] Another object of the present invention is to provide a high-yield Agrocybe chaxini cultivation method, which is obtained by cultivating the high-yield Agrocybe chaxini strain described above, and the cultivation method comprises: Step 1, strain preparation; Step 2, cultivation medium preparation and inoculation; Step 3, germ management; Step 4: Mushroom management.

[0012] In step 1, the spawn preparation includes: taking out the high-yield Agrocybe chaxini spawn, transferring it to PDA culture medium in a clean bench, sealing it with sealing film, and placing it in a constant temperature incubator at 25°C in the dark for static cultivation. After the mycelium has covered the plate, the spawn is taken out for later use; In step 2, the cultivation medium preparation and inoculation include: Preparation of cultivation medium: The cultivation medium consists of coarse materials, fine materials and water; By mass ratio, the coarse material is 28.5-29.5% rape straw, 14.5-15.5% sawdust, 21-22% wheat bran, 28.5-29.5% rice husk; the fine material is 0.5-1.5% sucrose, 0.5-1.5% gypsum, 1.5-2.5% lime, and the rest is water; wherein the ratio of the sum of the coarse material and the fine material to the water is 2:3; Inoculate the cultured solid bacteria block into the sterilized bacteria bag and culture in the dark at 25℃; Place the cooled fungus bag in a sterile clean bench, inoculate the prepared fungus with an inoculation needle, and inoculate 3 fungus blocks of (2.5-3.5) cm × (1.4-1.6) cm in size into each fungus bag and evenly distribute them on the top of the culture medium.

[0013] In step 3, the spawn management includes: placing the inoculated bags in a constant temperature fruiting room for light-proof cultivation. The temperature in the fruiting room is set at 24-26°C and the relative humidity is set at 65-75%. When the mycelium has grown to the top, remove the sealing film and continue spawning until the mycelium has grown to the top of the bag.

[0014] In step 4, fruiting management includes: Adjust the temperature to 21-23℃ and use light to stimulate primordium differentiation. After opening the bags, arrange the mushrooms in random blocks and carry out fruiting management on the shelves. Maintain the relative humidity of the air at 90%-95% during the fruiting period. Harvest the Agrocybe chaxini when the fruiting bodies of the Agrocybe chaxini grow and the fungus film is not broken.

[0015] In combination with all the above technical solutions, the beneficial effects of the present invention are as follows: First, the strain CXG001 is a high-yielding variety. Under the same planting conditions, it achieves a 71.6% yield increase over Cha 3 (Chaxingu 3) and a 28.1% yield increase over Cha 5 (Chaxingu 5). This strain can replace older varieties such as Cha 3 and Cha 5.

[0016] Second, under equivalent cultivation conditions, the present strain of Agrocybe chaxingensis has a yield that is 28%-71% higher than existing strains. This significant yield increase is significant, and at equivalent costs, the yield is higher than existing strains. The technical solution of the present invention is expected to yield significant returns after implementation. Under equivalent conditions, producers can expect to increase their income by over 20%. The strain of the present invention has a yield that is over 20% higher than existing strains.

[0017] Third, the high-yield strain provided by the present invention is a high-yield strain domesticated and cultivated by the inventors after extensive basic research, and spending a lot of time and energy through a large number of resource surveys and cultivation experiments, which solves the problem of the lack of high-yield, short-cycle, high-quality varieties in the Agrocybe aegerita industry. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present disclosure and, together with the description, serve to explain the principles of the present disclosure; Figure 1 This is a flow chart of a method for cultivating a high-yield Agrocybe chaxini strain provided by an embodiment of the present invention; Figure 2 The present invention provides a method for combining the LSU and TEF1α sequences of strain CXG001 and constructing a Cyclocybe Schematic diagram of the molecular phylogenetic tree of the genus; Figure 3 This is a rendering of the effect of the high-yield Agrocybe chaxini strain provided by an embodiment of the present invention. DETAILED DESCRIPTION

[0019] To make the above-mentioned objects, features, and advantages of the present invention more readily apparent, specific embodiments of the present invention are described in detail below with reference to the accompanying drawings. The following description sets forth numerous specific details to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways than those described herein, and those skilled in the art may make similar modifications without departing from the scope of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0020] Example 1: The high-yield Agrocybe chaxini strain provided in the present invention is strain CXG001, which is deposited in the China Center for Type Culture Collection, Wuhan University, Wuhan, China, with a deposit number of CCTCC NO: M 20251055 and a deposit date of May 14, 2025. The test result is: survival, and the classification name is: Cyclocybe chaxingu CXG001.

[0021] Example 2, as Figure 1 As shown, the embodiment of the present invention provides a method for culturing a high-yield Agrocybe chaxini strain, comprising: S1, remove the roots of wild-type Agrocybe CXG001, remove the soil and impurities on the surface with alcohol cotton, and disinfect; S2, cutting the wild-type Agrocybe CXG001 fruiting body longitudinally to obtain the fruiting body tissue block, placing it in the prepared PDA culture medium, and after mycelium grows, picking the PDA culture medium containing mycelium and culturing it in a new PDA culture dish, repeating the operation multiple times; S3. When the mycelium has grown completely over the culture dish, small cubes of culture medium containing mycelium are cut out, and multiple cubes of culture medium are placed into a cryopreservation tube containing sterile water. The cryopreservation tube is sealed with a cap for storage.

[0022] An exemplary method for cultivating a high-yield Agrocybe chaxini strain specifically includes: The wild-type Agrocybe CXG001 was collected from Nanchang, Jiangxi Province, on a rotten wood substrate. Its fruiting bodies were collected and brought back to the laboratory. Agrocybe CXG001 was isolated using a tissue separation method. The detailed steps are as follows: The roots of Agrocybe CXG001 collected from the wild were removed, and the surface soil and other impurities were removed with 75% alcohol cotton.

[0023] Put the processed fruiting bodies into sampling bags, write the numbers on them and take them back to the laboratory.

[0024] Operate in a clean bench sterilized with ultraviolet light: disinfect the surface of the fruiting body with 75% alcohol cotton, cut the fruiting body longitudinally with a knife, use sterilized tweezers to clamp a small piece of fruiting body tissue at the connection between the cap and the stipe, place it in the prepared PDA culture medium, observe for 2-5 days, and immediately use a sterilized needle to pick up the PDA culture medium containing mycelium and culture it in a new PDA culture dish after the mycelium grows. After the mycelium grows, use a sterilized needle to pick up the PDA culture medium containing mycelium again and culture it in a new dish containing PDA culture medium. When the mycelium fills the culture dish, use a sterilized knife to cut small cubes of culture medium containing mycelium with a side length of 1-2 mm, take 5-8 small pieces and place them in a cryopreservation tube containing sterile water, seal the cryopreservation tube cap, and store at 4°C.

[0025] Example 3, as a possible embodiment of the present invention, provides an application of a high-yield Agrocybe chaxini strain as a parent in the breeding of high-yield Agrocybe chaxini, and uses the high-yield Agrocybe chaxini strain for breeding.

[0026] Example 4, as a possible implementation mode of the present invention, the present invention provides a high-yield Agrocybe chaxin mycelium, which is obtained by culturing the high-yield Agrocybe chaxin strain.

[0027] Example 5, as a possible embodiment of the present invention, the present invention provides a high-yield Agrocybe chaxini fruiting body, which is obtained by culturing the high-yield Agrocybe chaxini strain.

[0028] Example 6, identification of strain CXG001.

[0029] 1. Morphological identification of CXG001 strain.

[0030] Colony morphology: On PDA culture medium, the hyphae of the colony are velvety, round, convex in the middle, white on the front, and brown spots on the back. The aerial hyphae are thin and white, and grow rapidly at a rate of 0.6–0.8 cm / d.

[0031] Fruiting body morphology: The cap is 20-120.5mm in diameter, initially hemispherical, then convex to flat. When young, it is dark brown, gradually fading to brown to light brown, darker in the center and lighter to nearly white at the edges. It is non-sticky, with a smooth or wrinkled surface. The flesh is white, thick in the middle and thinner at the edges, with a strong flavor and tea-tea aroma. The stipe is mesenteral, 44-126mm long, nearly cylindrical, and dirty white. The surface is striped with longitudinal fibers in the middle, becoming thinner at the ends, and the interior is solid. The annulus is thin, membranous, and single, initially white, and then bears brown spores on the upper surface, which may persist or shed easily. The spore print is brown.

[0032] 2. Molecular identification of strain CXG001.

[0033] 2.1. Extract DNA.

[0034] The specific steps for DNA extraction are as follows: 1) Scrape approximately 500 mg of mycelium from the culture dish (avoid scraping the culture medium), add 1 mL of 2× CTAB lysis buffer preheated at 60°C, and grind with a grinding rod (add 500 μL of 2× CTAB lysis buffer twice, first adding 500 μL of 2× CTAB lysis buffer; after grinding, add 500 μL of 2× CTAB lysis buffer again to thoroughly grind). After thorough grinding, transfer to a centrifuge tube and incubate in a water bath for 30-60 minutes. 2) Add 500 mL of chloroform / isoamyl alcohol (24:1) solution, mix well, and centrifuge at 11,400 rpm for 15 min (room temperature). 3) Remove the supernatant (about 500 mL, be careful not to aspirate the precipitate), add an equal volume of chloroform / isoamyl alcohol solution, mix well, and centrifuge at 11,400 rpm for 15 minutes (room temperature); 4) Remove the supernatant (about 400 mL) and repeat step 3. 5) Remove the supernatant (approximately 300 mL), add 10% of the total volume of 5 M KOAc solution, add 2 / 3 of the total volume of isopropanol, mix well, and let stand. 6) Freeze at -20°C overnight and centrifuge at 11,400 rpm for 5 min; 7) Discard the liquid phase (be careful not to discard the precipitate), add 400 mL of 70% ethanol, shake and wash, and centrifuge at 11,400 rpm for 5 minutes; 8) Repeat step 7); 9) Discard the liquid phase (be careful not to discard the precipitate). After the DNA is dried, add 50-100 μL of 1×TE buffer, dissolve the DNA at 37°C, and store at -20°C until use.

[0035] 2.2. PCR amplification and sequencing.

[0036] Polymerase chain reaction (PCR) was used to amplify the 28S ribosomal large subunit (LSU) gene and the translation elongation factor 1-alpha (TEF1α) gene. The forward and reverse primers used in this invention were LROR / LR5 (forward / reverse) for LSU and EF1-α / EF1-1620R for TEF1α. Primer synthesis and PCR product sequencing were provided by Beijing Qingke Biotechnology Co., Ltd. (Changsha Branch). The specific base sequences are as follows: LR0R: GTACCCGCTGAACTTAAGC (SEQ ID NO: 3) (Reference: Cai L, Lumyong P, Zhang K, et al. New species of Annulatascus and Saccardoella from thePhilippines[J]. Mycotaxon, 2002, 84:255-263.1994); LR5: ATCCTGAGGGAAACTTC (SEQ ID NO: 4) (Reference: Cai L, Lumyong P, Zhang K, et al. New species of Annulatascus and Saccardoella from thePhilippines[J]. Mycotaxon, 2002, 84:255-263.1994); EF1-983F: GCYCCYGGHCAYCGTGAYTTYAT (SEQ ID NO: 5), (wherein Y represents the base C or T, and H represents the base A, C, or T. Reference: TEKPINAR AD, KALMER A. Utility of various molecular markers in fungal identification and phylogeny[J]. Nova Hedwigia, 2019, 109(1-2): 187-224); EF1-2218R: ATGACACCRACRGCRACRGTYTG (SEQ ID NO: 6) (wherein R represents base A or G, reference: TEKPINAR AD, KALMER A. Utility of various molecular markers in fungal identification and phylogeny[J]. Nova Hedwigia, 2019, 109 (1-2): 187-224).

[0037] 2.3. PCR amplification system.

[0038] Amplification reaction system: 2×Taq PCR Master Mix 12.5 µL, template DNA 1 µL, upper primer 1 µL, lower primer 1 µL, DdH2O 9.5 µL; Amplification procedures, including: LSU amplification program: pre-denaturation at 95°C for 3 min; denaturation at 94°C for 30 s, annealing at 55°C for 50 s, annealing at 72°C for 1 min, 35 cycles; extension at 72°C for 1 min, storage at 4°C; TEF1-α amplification procedure: pre-denaturation at 96 °C for 2 min; denaturation at 96 °C for 45 s, annealing at 52 °C for 30 s, annealing at 72 °C for 1.5 min, 40 cycles; extension at 72 °C for 5 min, and storage at 4 °C; The PCR products were sent to Beijing Qingke Biotechnology Co., Ltd. for sequencing, and LSU and TEF1α sequences were obtained. The nucleotide sequences are shown in SEQ ID NO: 1 and SEQ ID NO: 2.

[0039] LSU sequence, SEQ ID NO: 1: ; TEF1α sequence, SEQ ID NO: 2: .

[0040] The LSU and TEF1α sequences of strain CXG001 were combined and constructed with the reference sequence Cyclocybe Molecular phylogenetic tree of the genus ( Figure 2 ). From the phylogenetic tree, we can see that the strain CXG001 and Agrocybe chaxingensis ( Cyclocybe chaxingu ) reference sequence on the same branch, and the support rate is very high (99%), therefore, it can be determined that the strain CXG001 is Agrocybe chaxing. The strain CXG001 of the present invention has significant genetic uniqueness from other Agrocybe chaxing strains.

[0041] In Example 7, the present invention provides a method for cultivating a high-yield Agrocybe chaxini, which is obtained by cultivating the high-yield Agrocybe chaxini strain. The method comprises: Step 1, strain preparation; Step 2, cultivation medium preparation and inoculation; Step 3, germ management; Step 4: Mushroom management.

[0042] Exemplarily, in step 1, bacterial strains are prepared.

[0043] The preserved CXG001 Agrocybe chaxing strain was taken out and transferred to PDA culture medium in a clean bench. The medium was sealed with sealing film and placed in a constant temperature incubator at 25°C for static culture in the dark. The plate was taken out for later use after the mycelium covered the entire plate.

[0044] Exemplarily, in step 2, the cultivation medium is prepared and inoculated.

[0045] The cultivation material formula is as follows: Coarse material: rapeseed straw 28.5-29.5%, sawdust 14.5-15.5%, wheat bran 21-22%, rice husk 28.5-29.5%; fine material: sucrose 0.5-1.5%, gypsum 0.5-1.5%, lime 1.5-2.5%; material: water 2:3. Inoculate the cultured solid mass into a sterilized bag and incubate in the dark at 25°C.

[0046] The bag size is 18 cm × 36 cm × 0.005 cm, and each bag can hold 470-475 kg. Place the cooled bags in a sterile clean bench and inoculate the prepared spawn using an inoculation needle. Place three (2.5-3.5) cm × (1.4-1.6) cm clumps of spawn into each bag, evenly spaced on top of the culture medium. Maintain aseptic technique during inoculation.

[0047] Exemplarily, in step 3, sterilization management.

[0048] After inoculation, place the bags in a constant-temperature fruiting room for incubation in the dark. Set the temperature to 24-26°C and the relative humidity to 65-75%. During the early stages of incubation, as the mycelium begins to germinate, avoid frequent entry and exit of the fruiting room to inspect the bags. After about a week of mycelium germination, turn the bags over to check for contamination. Dispose of any contaminated bags promptly to prevent contamination of other bags. After ten days, when the mycelium has grown to the top, remove the sealing film and continue inoculation. Approximately 45 days after inoculation, the bags will be fully filled with mycelium.

[0049] Exemplarily, in step 4, mushroom production management.

[0050] Adjust the temperature to 21-23°C and provide light to stimulate primordium differentiation. Open the bags after 10 days. Arrange the mushrooms in randomized blocks and perform fruiting management on the shelves. Maintain a relative humidity of 90%-95% during fruiting. Maintain humidity by spraying water on the ground, the air, and the walls. Avoid spraying water onto the bags, as this can easily cause contamination. Harvest the Agrocybe aegerita fruiting bodies immediately after they emerge, when the edges of the caps are lighter in color and the mycelium is intact or slightly broken.

[0051] Example 8, measurement and analysis of Agrocybe chaxingu yield.

[0052] 1. Yield and dry weight of Agrocybe chaxingensis.

[0053] The first batch of fresh mushrooms and the second batch of fresh mushrooms of each bag of Agrocybe aegerita were weighed and recorded respectively.

[0054] Yield of single bag of first batch mushrooms: refers to the fresh weight of the first batch of Agrocybe chaxini fruiting bodies; The yield of the second batch of mushrooms per bag is the fresh weight of the second batch of Agrocybe chaxini fruiting bodies; Total yield (total yield of two crops of mushrooms): the sum of the fresh weight of the first crop of mushrooms and the fresh weight of the second crop of mushrooms.

[0055] 2. Biological efficiency.

[0056] The biological efficiency of each fungus bag is calculated according to the following formula: Biological efficiency = (total yield of two mushroom crops / dry weight of cultivation medium) × 100%. Data analysis is shown in Table 1.

[0057] Table 1 Data analysis table

[0058] in, Figure 3 This is a rendering of the effect of the high-yield Agrocybe chaxini strain provided by an embodiment of the present invention.

[0059] In the above embodiments, the description of each embodiment has its own focus. For parts that are not described or recorded in detail in a certain embodiment, reference can be made to the relevant description of other embodiments.

[0060] The above description is only a preferred specific implementation method of the present invention, but the scope of protection of the present invention is not limited thereto. Any modifications, equivalent substitutions and improvements made by any technician familiar with this technical field within the technical scope disclosed by the present invention and within the spirit and principles of the present invention should be covered by the scope of protection of the present invention.

Claims

1. A high-yield Agrocybe chaxini strain, characterized in that: The high-yield Agrocybe chaxini strain is strain CXG001, which is deposited in the China Center for Type Culture Collection with a deposit number of CCTCC NO: M 20251055 and a deposit date of May 14, 2025. It is classified and named as: Cyclocybe chaxingu CXG001.

2. A method for cultivating a high-yield Agrocybe chaxini strain, characterized in that: The culture method is used to culture the high-yield Agrocybe chaenomeles strain according to claim 1, and the culture method comprises: S1, remove the roots of wild-type Agrocybe CXG001, remove the soil and impurities on the surface with alcohol cotton, and disinfect; S2, cutting the wild-type Agrocybe CXG001 fruiting body longitudinally to obtain the fruiting body tissue block, placing it in the prepared PDA culture medium, and after mycelium grows, picking the PDA culture medium containing mycelium and culturing it in a new PDA culture dish, repeating the operation multiple times; S3. When the mycelium has grown completely over the culture dish, small cubes of culture medium containing mycelium are cut out, and multiple cubes of culture medium are placed into a cryopreservation tube containing sterile water. The cryopreservation tube is sealed with a cap for storage.

3. The method for cultivating a high-yield Agrocybe chaxini strain according to claim 2, characterized in that: In step S3, small cubes of culture medium containing mycelia with a side length of 1-2 mm are cut and stored at 4°C.

4. Application of a high-yield Agrocybe chaxini strain as a parent in breeding high-yield Agrocybe chaxini, characterized in that: The high-yield Agrocybe chaxini strain according to claim 1 is used for breeding.

5. A high-yield Agrocybe chaxini mycelium, characterized in that: The high-yield Agrocybe chaxini strain according to claim 1 is used for cultivation.

6. A high-yield Agrocybe chaxinensis fruiting body, characterized in that: The high-yield Agrocybe chaxini strain according to claim 1 is used for cultivation.

7. A high-yield cultivation method of Agrocybe chaxini, characterized in that: The high-yield Agrocybe chaxini strain according to claim 1 is cultivated, and the cultivation method comprises: Step 1, strain preparation; Step 2, cultivation medium preparation and inoculation; Step 3, germ management; Step 4: Mushroom management.

8. The high-yield cultivation method of Agrocybe chaxini according to claim 7, characterized in that: In step 1, the spawn preparation includes: taking out the high-yield Agrocybe chaxini spawn, transferring it to PDA culture medium in a clean bench, sealing it with sealing film, and placing it in a constant temperature incubator at 25°C in the dark for static cultivation. After the mycelium has covered the plate, the spawn is taken out for later use; In step 2, the cultivation medium preparation and inoculation include: Preparation of cultivation medium: The cultivation medium consists of coarse materials, fine materials and water; By mass ratio, the coarse material is 28.5-29.5% rape straw, 14.5-15.5% sawdust, 21-22% wheat bran, 28.5-29.5% rice husk; the fine material is 0.5-1.5% sucrose, 0.5-1.5% gypsum, 1.5-2.5% lime, and the rest is water; wherein the ratio of the sum of the coarse material and the fine material to the water is 2:3; Inoculate the cultured solid bacteria block into the sterilized bacteria bag and culture in the dark at 25℃; Place the cooled fungus bag in a sterile clean bench, inoculate the prepared fungus with an inoculation needle, and inoculate 3 fungus blocks of (2.5-3.5) cm × (1.4-1.6) cm in size into each fungus bag and evenly distribute them on the top of the culture medium.

9. The high-yield cultivation method of Agrocybe chaxingensis according to claim 7, characterized in that: In step 3, the spawn management includes: placing the inoculated bags in a constant temperature fruiting room for light-proof cultivation. The temperature in the fruiting room is set at 24-26°C and the relative humidity is set at 65-75%. When the mycelium has grown to the top, remove the sealing film and continue spawning until the mycelium has grown to the top of the bag.

10. The high-yield cultivation method of Agrocybe chaxingensis according to claim 7, characterized in that: In step 4, fruiting management includes: Adjust the temperature to 21-23℃ and use light to stimulate primordium differentiation. After opening the bags, arrange the mushrooms in random blocks and carry out fruiting management on the shelves. Maintain the relative humidity of the air at 90%-95% during the fruiting period. Harvest the Agrocybe chaxini when the fruiting bodies of the Agrocybe chaxini grow and the fungus film is not broken.

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