Test strip for detecting mites, application of test strip and kit for detecting mites

Abstract

The invention relates to the technical field of detection reagents, in particular to a test strip for detecting mites, application of the test strip and a kit for detecting the mites. The test strip comprises a sample pad, a combination pad, a nitrocellulose membrane and absorbent paper, the combination pad is coated with an anti-mite antibody marked by colloidal gold; the nitrocellulose membrane is provided with a detection line and a quality control line, the detection line is coated with a mite antigen, and the quality control line is coated with a goat anti-rabbit IgG antibody. The test strip is prepared by using a colloidal gold method, and the adopted principle is a double-antibody sandwich method. By utilizing the test strip disclosed by the invention, whether the acarid is contained or not can be obtained within 5-10 minutes without an extra detection instrument, and the detection method is high in accuracy. The possibility is provided for efficiently and quickly detecting the mites.

Description

Technical Field

[0001] The present invention relates to the technical field of detection reagents, and in particular to a test strip for detecting mites, applications thereof, and a kit for detecting mites. Background Art

[0002] Mites are widely distributed.

[0003] Mites can feed on the blood of animals and humans, spreading a variety of diseases. Some mites can even feed on bodily fluids, causing inflammation and other illnesses. Mites in the environment can also cause asthma. Clearly, mites pose a threat to both animals and humans. Detection of mites is urgent.

[0004] However, existing methods for detecting mites include microscopic observation and ELISA, which are complex, time-consuming, and require specialized personnel or equipment. Therefore, a rapid, efficient, and suitable method for home or on-site detection is needed to address the shortcomings of existing technologies. Summary of the Invention

[0005] The purpose of the present invention is to provide a rapid, efficient method that does not require any detection instrument and is suitable for home or on-site detection; in particular, it relates to a test strip for detecting mites, its application, and a kit for detecting mites.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The present invention provides a test strip for detecting mites, the test strip comprising a sample pad, a conjugate pad, a nitrocellulose membrane, and absorbent paper;

[0008] The conjugate pad is coated with anti-mite antibodies labeled with colloidal gold;

[0009] A detection line and a quality control line are provided on the nitrocellulose membrane. The detection line is coated with mite antigens, and the quality control line is coated with goat anti-rabbit IgG antibodies.

[0010] Preferably, the preparation method of the colloidal gold-labeled anti-mite antibody is as follows:

[0011] A chloroauric acid solution and a trisodium citrate solution are mixed and heated until the solution turns red to obtain a colloidal gold solution; the pH of the colloidal gold solution is adjusted to 8.0-8.5, an anti-mite antibody is added, and the mixture is stirred for 20-40 minutes to obtain a colloidal gold antibody solution; the colloidal gold antibody solution is mixed with bovine serum albumin, stirred for 15-25 minutes, centrifuged for 20-40 minutes, the supernatant is discarded, and the precipitate is dried to obtain a colloidal gold-labeled anti-mite antibody.

[0012] Preferably, the initial concentration of the chloroauric acid solution is 0.01-0.02 wt %; the initial concentration of the trisodium citrate solution is 0.5-1.5 wt %;

[0013] The volume ratio of the chloroauric acid solution to the trisodium citrate solution is 20 to 50:1;

[0014] The reagent for adjusting the pH of the colloidal gold solution is a potassium carbonate solution, and the concentration of the potassium carbonate solution is 0.05 to 0.2 mol / L;

[0015] The anti-mite antibody is added at a final concentration of 0.8 to 1.2 mg / mL;

[0016] The mass volume ratio of the mixture of bovine serum albumin and colloidal gold antibody solution is 8-12 g:100 mL;

[0017] The centrifugal speed is 10000-15000 rpm.

[0018] Preferably, the sample pad is a treated sample pad;

[0019] The sample pad treatment method is as follows: placing a blank sample pad in a sample pad treatment solution, soaking for 10 to 20 minutes, and drying to obtain a sample pad;

[0020] The sample pad treatment solution uses water as a solvent and includes the following components in the following concentrations:

[0021] Disodium hydrogen phosphate dodecahydrate 5-6g / L, sodium dihydrogen phosphate dihydrate 0.5-1.0g / L;

[0022] The drying temperature is 40-50° C., and the drying time is 7-8 hours.

[0023] Preferably, the conjugate pad is a treated conjugate pad;

[0024] The conjugate pad treatment method is as follows: placing a blank conjugate pad in a PBS solution containing bovine serum albumin, soaking for 30 to 60 minutes, and drying to obtain a conjugate pad;

[0025] The final concentration of the bovine serum albumin is 0.5-1.5 wt%;

[0026] The drying temperature is 40-50°C and the drying time is 7-8h;

[0027] The initial solubility of the anti-mite antibody labeled with colloidal gold coated on the conjugate pad is 0.5-1.5 mg / mL.

[0028] Preferably, the concentration of the coated mite antigen on the test line is 0.8 to 1.2 mg / mL;

[0029] The concentration of goat anti-rabbit IgG antibody coated on the quality control line was 0.5-1.5 mg / mL.

[0030] The present invention also provides the use of the test strip in preparing a kit for accurately detecting mites.

[0031] The present invention also provides a method for detecting mites for a non-disease diagnosis purpose, comprising the following steps:

[0032] (1) Mix the sample to be tested with RIPA lysis buffer for 10-20 minutes to obtain the substance to be tested;

[0033] (2) Add the test substance to the sample well of the test strip, wait 5 to 10 minutes, and observe the results;

[0034] The test strip is the test strip described above.

[0035] Preferably, the sample to be tested is blood, saliva, urine, sweat, hair follicle secretion or dust;

[0036] The RIPA lysis solution also includes Triton with a final concentration of 0.3 to 0.8 wt %;

[0037] The method for judging the results is: if both the test line and the quality control line of the test strip are colored, the test result is positive and contains mite antigen; if only the quality control line of the test strip is colored, the test result is negative and does not contain mite antigen; if the quality control line of the test strip is not colored, the test result is invalid and needs to be retested.

[0038] The present invention also provides a kit for detecting mites, comprising RIPA lysis solution and a test strip;

[0039] The RIPA lysis solution includes Triton with a final concentration of 0.3 to 0.8 wt %;

[0040] The test strip is the test strip described above.

[0041] The present invention provides a test strip for detecting mites, its application, and a kit for detecting mites. The test strip of the present invention comprises a sample pad, a conjugation pad, a nitrocellulose membrane, and blotting paper; the conjugation pad is coated with anti-mite antibodies labeled with colloidal gold; the nitrocellulose membrane is provided with a detection line and a quality control line, the detection line is coated with a mite antigen, and the quality control line is coated with a goat anti-rabbit IgG antibody. The test strip of the present invention is prepared using a colloidal gold method, and the principle adopted is a double antibody sandwich method. Using the test strip of the present invention, it is possible to determine whether mites are present within 5 to 10 minutes without the need for additional detection equipment, and the detection method is highly accurate. This provides the possibility for efficient and rapid detection of mites. DETAILED DESCRIPTION

[0042] The present invention provides a test strip for detecting mites, the test strip comprising a sample pad, a conjugate pad, a nitrocellulose membrane, and absorbent paper;

[0043] The conjugate pad is coated with anti-mite antibodies labeled with colloidal gold;

[0044] A detection line and a quality control line are provided on the nitrocellulose membrane. The detection line is coated with mite antigens, and the quality control line is coated with goat anti-rabbit IgG antibodies.

[0045] In the present invention, the sample pad, conjugate pad, nitrocellulose membrane and absorbent paper in the test strip are prepared by bonding them in sequence.

[0046] In the present invention, the preparation method of the colloidal gold-labeled anti-mite antibody is as follows:

[0047] A chloroauric acid solution and a trisodium citrate solution are mixed and heated until the solution turns red to obtain a colloidal gold solution; the pH of the colloidal gold solution is adjusted to 8.0-8.5, an anti-mite antibody is added, and the mixture is stirred for 20-40 minutes to obtain a colloidal gold antibody solution; the colloidal gold antibody solution is mixed with bovine serum albumin, stirred for 15-25 minutes, centrifuged for 20-40 minutes, the supernatant is discarded, and the precipitate is dried to obtain a colloidal gold-labeled anti-mite antibody.

[0048] In the present invention, the initial concentration of the chloroauric acid solution is 0.01-0.02 wt%, preferably 0.015 wt%; the initial concentration of the trisodium citrate solution is 0.5-1.5 wt%, preferably 1.0 wt%;

[0049] The volume ratio of the chloroauric acid solution to the trisodium citrate solution is 20 to 50:1, preferably 35:1;

[0050] The reagent for adjusting the pH of the colloidal gold solution is potassium carbonate solution, and the concentration of the potassium carbonate solution is 0.05-0.2 mol / L, preferably 0.1 mol / L; the adjusted pH is preferably 8.2.

[0051] The final concentration of the anti-mite antibody added is 0.8-1.2 mg / mL, preferably 1.0 mg / mL; the stirring time is preferably 30 min.

[0052] The mass volume ratio of the mixture of bovine serum albumin and colloidal gold antibody solution is 8-12g:100mL; the centrifugal speed is 10000-15000rpm, preferably 12000rpm; the centrifugal time is preferably 30min; in the present invention, the drying is freeze drying.

[0053] In the present invention, the sample pad is a treated sample pad;

[0054] The sample pad treatment method is as follows: placing a blank sample pad in a sample pad treatment solution, soaking for 10 to 20 minutes, and drying to obtain a sample pad;

[0055] The sample pad treatment solution uses water as a solvent and includes the following components in the following concentrations:

[0056] Disodium hydrogen phosphate dodecahydrate 5-6 g / L, preferably 5.73 g / L;

[0057] Sodium dihydrogen phosphate dihydrate 0.5-1.0 g / L, preferably 0.62 g / L;

[0058] The soaking time is preferably 15 minutes;

[0059] The drying temperature is 40-50°C, preferably 45°C;

[0060] The drying time is 7 to 8 hours, preferably 7.5 hours.

[0061] In the present invention, the conjugate pad is a treated conjugate pad;

[0062] The conjugate pad treatment method is as follows: placing a blank conjugate pad in a PBS solution containing bovine serum albumin, soaking for 30 to 60 minutes, and drying to obtain a conjugate pad;

[0063] The final concentration of the bovine serum albumin is 0.5-1.5 wt%, preferably 1.0 wt%;

[0064] The soaking time is preferably 45 minutes;

[0065] The drying temperature is 40-50°C, preferably 45°C;

[0066] The drying time is 7 to 8 hours, preferably 7.5 hours.

[0067] The initial solubility of the colloidal gold-labeled anti-mite antibody coated on the conjugate pad is 0.5-1.5 mg / mL, preferably 1.0 mg / mL. The anti-mite antibody is sprayed onto the conjugate pad at a rate of 2-6 μl / mL, preferably 4 μl / mL. The solution of the colloidal gold-labeled anti-mite antibody is diluted with water and includes the following components at the following concentrations: 3 wt% bovine serum albumin, 10-20 wt% sucrose, 0.5-1.0 wt% sodium chloride, and 0.5-1.0 wt% Tween-20.

[0068] In the present invention, the concentration of the coated mite antigen on the test line is 0.8 to 1.2 mg / mL, preferably 1.0 mg / mL; the line speed when coated with the mite antigen is 1 to 8 m / s, preferably 5 m / s;

[0069] The concentration of the goat anti-rabbit IgG antibody coated on the quality control line is 0.5-1.5 mg / mL, preferably 1.2 mg / mL; the marking speed when coating the goat anti-rabbit IgG antibody is 1-8 m / s, preferably 5 m / s.

[0070] The present invention also provides the use of the test strip in preparing a kit for accurately detecting mites.

[0071] The present invention also provides a method for detecting mites for a non-disease diagnosis purpose, comprising the following steps:

[0072] (1) Mix the sample to be tested with RIPA lysis buffer for 10-20 minutes to obtain the substance to be tested;

[0073] (2) Add the test substance to the sample well of the test strip, wait 5 to 10 minutes, and observe the results;

[0074] The test strip is the test strip described above.

[0075] In the present invention, the sample to be tested is blood, saliva, urine, sweat, hair follicle secretion or dust;

[0076] The RIPA lysis buffer further comprises Triton at a final concentration of 0.3-0.8 wt%, preferably 0.5 wt%;

[0077] The method for judging the results is: if both the test line and the quality control line of the test strip are colored, the test result is positive and contains mite antigen; if only the quality control line of the test strip is colored, the test result is negative and does not contain mite antigen; if the quality control line of the test strip is not colored, the test result is invalid and needs to be retested.

[0078] The present invention also provides a kit for detecting mites, comprising RIPA lysis solution and a test strip;

[0079] The RIPA lysis buffer includes Triton at a final concentration of 0.3-0.8 wt%, preferably 0.5 wt%;

[0080] The test strip is the test strip described above.

[0081] In the present invention, the mites detected by the present invention are preferably dust mites.

[0082] The technical solutions provided by the present invention are described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0083] The blank sample pad in the embodiment of the present invention is glass cellulose membrane-GL0194;

[0084] The blank conjugate pad in the embodiment of the present invention is a polyester cellulose membrane-MA0120;

[0085] The blank nitrocellulose membrane in the embodiment of the present invention is HATF00010.

[0086] Example 1

[0087] Preparation of anti-mite antibodies

[0088] Cultured dust mites were ground in liquid nitrogen to obtain a sample. Coca-Cola alkaline extract was added to the dust mite sample at a ratio of 1.0 g dust mite sample to 5 mL of Coca-Cola alkaline extract. Extraction was performed at 4°C for 50 hours to obtain an extract. The extract was filtered, and the filtrate was collected to obtain an extract solution. The extract solution was placed in a dialysis bag, which was then placed in the Coca-Cola alkaline extract solution and dialyzed at 4°C for 8 hours, with the dialysate replaced every four hours. The dialyzed extract solution was filtered and sterilized to obtain dust mite antigen. The detected concentration of this antigen was 4.5 mg / mL.

[0089] Dust mite antigen was diluted to 3 mg / mL in Freund's adjuvant and administered subcutaneously to rabbits, with 1 mL administered each time. Booster immunizations were performed one week apart at the same dose. Five days after the final immunization, blood was collected from the orbital vein, and serum was separated and purified by affinity chromatography to obtain dust mite antibodies. The eluent for purification was 1 M phosphate buffer.

[0090] The concentration of dust mite antibodies detected was 3.8 mg / mL.

[0091] Example 2

[0092] Test strips for detecting dust mites

[0093] Dissolving chloroauric acid in distilled water to prepare a chloroauric acid solution with a concentration of 0.015 wt %;

[0094] Trisodium citrate was dissolved in distilled water to prepare a trisodium citrate solution with a concentration of 1.0 wt %.

[0095] Take 30 mL of the prepared chloroauric acid solution, heat it to 100°C, add 1 mL of trisodium citrate solution, continue heating and stirring until the mixed solution turns red, and obtain a colloidal gold solution. After cooling to room temperature, adjust the pH of the colloidal gold solution to 8.2 using 0.1 mol / L potassium carbonate solution, add the dust mite antibody prepared in Example 1, and add the dust mite antibody to a final concentration of 1.0 g / mL; stir at 200 rpm for 30 minutes to obtain a colloidal gold antibody solution. Mix bovine serum albumin and the colloidal gold antibody solution at a mass-to-volume ratio of 10 g:100 mL, stir at 200 rpm for 20 minutes, centrifuge at 12,000 rpm for 30 minutes, discard the supernatant, and freeze-dry the precipitate to obtain colloidal gold-labeled anti-mite antibodies.

[0096] The colloidal gold-labeled anti-mite antibody was mixed with a solution of diluted colloidal gold-labeled anti-mite antibody to prepare a colloidal gold-labeled anti-mite antibody solution having a concentration of 1 mg / mL. The diluted colloidal gold-labeled anti-mite antibody solution contained water as a solvent and also included 3 wt% bovine serum albumin, 20 wt% sucrose, 1.0 wt% sodium chloride, and 1.0 wt% Tween-20.

[0097] The blank conjugate pad was placed in a PBS solution containing bovine serum albumin and soaked for 45 minutes. The blank conjugate pad was taken out and placed in a 45° C. constant temperature drying oven and dried for 8 hours to obtain a conjugate pad.

[0098] The colloidal gold-labeled anti-mite antibody solution was pipetted into a colloidal gold streaking apparatus, sprayed onto the conjugate pad at a rate of 4 μl / mL, dried, and set aside.

[0099] The sample pad treatment solution uses water as a solvent and further includes 5.73 g / L of disodium hydrogen phosphate dodecahydrate and 0.62 g / L of sodium dihydrogen phosphate dihydrate.

[0100] The blank sample pad was soaked in the sample pad treatment solution for 15 minutes, the sample pad was taken out, and placed in a constant temperature drying oven at 45° C. and dried for 8 hours to obtain a sample pad.

[0101] The dust mite antigen prepared in Example 1 was diluted to 1 mg / mL with PBS, and the goat anti-rabbit IgG antibody was diluted to 1 mg / mL with PBS. The diluted antigen and antibody were pipetted into the two marking holes of a colloidal gold streak apparatus, respectively. The streak speed was adjusted to 5 m / s, and a line was drawn on a nitrocellulose membrane. The area coated with the dust mite antigen served as the test line (T), and the area coated with the rabbit IgG antibody served as the control line (C). The membrane was dried and set aside.

[0102] The treated sample pad, the binding pad coated with colloidal gold-labeled anti-mite antibody, the scribed nitrocellulose membrane and the absorbent paper are sequentially pasted, cut and assembled to obtain a test strip.

[0103] Example 3

[0104] Dust was collected from five random locations on a household mattress using a sample brush, with 0.1 g collected from each location to obtain the test sample. The test sample was mixed with RIPA lysis buffer containing 0.5 wt% Triton X-ray dilution and shaken for 15 minutes to obtain the test substance. 30 μL of the test substance was added dropwise to the sample well of the test strip prepared in Example 2. Each sample location was tested three times, and the results were observed after 5 minutes.

[0105] Finally, the results showed that the T and C lines of each test strip at three sample points showed color, indicating a positive test result. The test strips at the remaining two sample points showed color only on the C line, indicating a negative test result. This indicates that three sample points contained dust mites, while two sample points did not.

[0106] The samples from the five test spots were sliced and observed under a microscope. The results were consistent with the test strips: mites were observed under the microscope in the three samples with positive test results, while no mites were observed in the two samples with negative test results.

[0107] This shows that the test strip of the present invention has accurate detection results.

[0108] Example 4

[0109] The test strips prepared above were placed in a 37° C. constant temperature incubator for 7 days, and then the test substances at the five sample points of Example 3 were detected.

[0110] The results showed that among the three sample spots that showed positive, the test strips of two sample spots showed positive, and the T line of the test strip of one sample spot showed color, but the C line did not show color, and the result was invalid; the two sample spots that showed negative were both negative.

[0111] The samples whose results were judged to be invalid were retested using a test strip that had not been accelerated at 37°C, and the test strip showed a positive result.

[0112] It can be seen that the test strip of the present invention is stable and can still accurately detect whether a sample contains mites after 7 days of acceleration.

[0113] Example 5

[0114] Ten patients clinically diagnosed with mite infestation presented with symptoms such as papules and wheals. Hair follicle secretions from these patients were mixed with RIPA lysis buffer containing 0.5 wt% Triton X-ray and shaken for 15 minutes to obtain the test substance. Fifty μL of the test substance was dripped into the sample wells of the test strip prepared in Example 2. Each sample point was tested three times, and the results were observed after 5 minutes.

[0115] The results showed that of the 10 patients identified as infested with mites, nine had strongly positive hair follicle secretions, with distinct T-line coloration. One patient had a weakly positive result, with less distinct T-line coloration. When the hair follicle secretions from the weakly positive patient were prepared and examined under a 400x microscope, only one mite could be observed per field of view.

[0116] It can be seen that the test strip of the present invention has high detection sensitivity.

[0117] Example 6

[0118] RIPA lysis solution containing 0.5 wt % Triton was placed in a dropping bottle, and the dropping bottle and the test strip prepared in Example 2 were packaged in an aluminum foil bag and sealed to obtain a test kit.

[0119] As can be seen from the above embodiments, the present invention provides a test strip for detecting mites, its application, and a kit for detecting mites. The test strip of the present invention comprises a sample pad, a conjugation pad, a nitrocellulose membrane, and blotting paper; the conjugation pad is coated with anti-mite antibodies labeled with colloidal gold; the nitrocellulose membrane is provided with a detection line and a quality control line, the detection line is coated with a mite antigen, and the quality control line is coated with a goat anti-rabbit IgG antibody. The test strip of the present invention is prepared using the colloidal gold method, and the principle adopted is a double antibody sandwich method. Using the test strip of the present invention, it is possible to determine whether mites are present within 5 to 10 minutes without the need for additional detection equipment, and the detection method is highly accurate. It provides the possibility for efficient and rapid detection of mites.

[0120] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A test strip for detecting mites, characterized in that: The test strip comprises a sample pad, a conjugate pad, a nitrocellulose membrane, and absorbent paper; The conjugate pad is coated with anti-mite antibodies labeled with colloidal gold; A detection line and a quality control line are provided on the nitrocellulose membrane. The detection line is coated with mite antigens, and the quality control line is coated with goat anti-rabbit IgG antibodies.

2. The test strip according to claim 1, characterized in that The preparation method of the colloidal gold-labeled anti-mite antibody is as follows: A chloroauric acid solution and a trisodium citrate solution are mixed and heated until the solution turns red to obtain a colloidal gold solution; the pH of the colloidal gold solution is adjusted to 8.0-8.5, an anti-mite antibody is added, and the mixture is stirred for 20-40 minutes to obtain a colloidal gold antibody solution; the colloidal gold antibody solution is mixed with bovine serum albumin, stirred for 15-25 minutes, centrifuged for 20-40 minutes, the supernatant is discarded, and the precipitate is dried to obtain a colloidal gold-labeled anti-mite antibody.

3. The test strip according to claim 2, characterized in that The initial concentration of the chloroauric acid solution is 0.01-0.02 wt %; the initial concentration of the trisodium citrate solution is 0.5-1.5 wt %; The volume ratio of the chloroauric acid solution to the trisodium citrate solution is 20 to 50:1; The reagent for adjusting the pH of the colloidal gold solution is a potassium carbonate solution, and the concentration of the potassium carbonate solution is 0.05 to 0.2 mol / L; The anti-mite antibody is added at a final concentration of 0.8 to 1.2 mg / mL; The mass volume ratio of the mixture of bovine serum albumin and colloidal gold antibody solution is 8-12 g:100 mL; The centrifugal speed is 10000-15000 rpm.

4. The test strip according to claim 3, characterized in that The sample pad is a treated sample pad; The sample pad treatment method is as follows: placing a blank sample pad in a sample pad treatment solution, soaking for 10 to 20 minutes, and drying to obtain a sample pad; The sample pad treatment solution uses water as a solvent and includes the following components in the following concentrations: Disodium hydrogen phosphate dodecahydrate 5-6g / L, sodium dihydrogen phosphate dihydrate 0.5-1.0g / L; The drying temperature is 40-50° C., and the drying time is 7-8 hours.

5. The test strip according to claim 1, characterized in that The conjugate pad is a treated conjugate pad; The conjugate pad treatment method is as follows: placing a blank conjugate pad in a PBS solution containing bovine serum albumin, soaking for 30 to 60 minutes, and drying to obtain a conjugate pad; The final concentration of the bovine serum albumin is 0.5-1.5 wt%; The drying temperature is 40-50°C and the drying time is 7-8h; The initial solubility of the anti-mite antibody labeled with colloidal gold coated on the conjugate pad is 0.5-1.5 mg / mL.

6. The test strip according to claim 1, characterized in that The concentration of coated mite antigen on the test line is 0.8-1.2 mg / mL; The concentration of goat anti-rabbit IgG antibody coated on the quality control line was 0.5-1.5 mg / mL.

7. Use of the test strip according to any one of claims 1 to 6 in preparing a kit for accurately detecting mites.

8. A method for detecting mites for purposes other than disease diagnosis, characterized in that: The steps include: (1) Mix the sample to be tested with RIPA lysis buffer for 10-20 minutes to obtain the substance to be tested; (2) Add the test substance to the sample well of the test strip, wait 5 to 10 minutes, and observe the results; The test strip is the test strip according to any one of claims 1 to 6.

9. The method according to claim 8, characterized in that The sample to be tested is blood, saliva, urine, sweat, hair follicle secretion or dust; The RIPA lysis solution also includes Triton with a final concentration of 0.3 to 0.8 wt %; The method for judging the results is: if both the test line and the quality control line of the test strip are colored, the test result is positive and contains mite antigen; if only the quality control line of the test strip is colored, the test result is negative and does not contain mite antigen; if the quality control line of the test strip is not colored, the test result is invalid and needs to be retested.

10. A kit for detecting mites, characterized in that: Includes RIPA lysis buffer and test strips; The RIPA lysis solution includes Triton with a final concentration of 0.3 to 0.8 wt %; The test strip is the test strip according to any one of claims 1 to 6.