Automatic cell counting equipment

Abstract

The present invention belongs to the field of cell counting technology, in particular to an automatic counting device for cell counting plates, including a counter, an inlet plate fixedly inserted into the inlet guard plate of the counter, openings respectively provided on both sides of the inlet plate, an adjustment mechanism provided in the inlet plate, the adjustment mechanism including a group of electric push rods respectively fixedly connected to the inner walls on both sides of the inlet guard plate, the output shaft of the electric push rods fixedly connected to the push plate located in the opening, and further including: an offset acquisition module for real-time monitoring of the initial insertion position and posture of the cell counting plate; a pressure acquisition module for monitoring the contact state and pressure distribution between the push plate and the counting plate. In the present invention, the offset acquisition module is used to monitor the initial insertion position and posture of the counting plate in real time, and the pressure acquisition module is used to monitor the contact state and pressure distribution between the push plate and the counting plate, and the control module is used to perform comprehensive analysis and adjustment, so as to accurately correct the position deviation of the counting plate, thereby improving the eccentric wear phenomenon.

Description

Technical Field

[0001] The present invention relates to the technical field of cell counting, in particular to an automatic cell counting device. Background Art

[0002] In biological research, it is sometimes necessary to measure the number of cells per unit volume. In existing counting methods, a cell counting chamber is generally used to measure the number of cells. The existing cell counting chamber consists of a box body and a base plate disposed in a recessed space of the box body. A gap exists between the bottom surface of the recessed space of the box body and the base plate. This gap serves as the counting chamber of the cell counting chamber. Sample liquid is introduced into the counting chamber for counting. When in use, trypan blue dye is first added, and then the cell counting chamber is inserted into the inlet of a cell counter for rapid counting.

[0003] During use, cell counting plates are usually manually held and inserted into the cell counter one by one, and then removed at regular intervals to complete the counting operation of multiple cell counting plates. The direction of each person's insertion and removal action is difficult to accurately control, which can easily cause eccentric wear between the cell counting plate and the inlet, and thus easily cause damage to the cell counting plate and the inlet. Therefore, corresponding improvements have been made to address this problem. Summary of the Invention

[0004] Based on the technical problems existing in the prior art, the present invention proposes an automatic cell counting device.

[0005] The automatic cell counting device proposed in the present invention includes a counter, wherein an inlet plate is fixedly inserted into an inlet guard plate at the inlet of the counter, openings are respectively provided on both sides of the inlet plate, and an adjustment mechanism is provided in the inlet plate, the adjustment mechanism includes a group of electric push rods respectively fixedly connected to the inner walls of the two sides of the inlet guard plate, and the output shaft of the electric push rod is fixedly connected to the push plate located in the opening, and further includes: an offset acquisition module, which is installed on the inlet plate near the inlet, and is used to monitor the initial insertion position and posture of the cell counting plate in real time, and generate a position offset through the control module; a pressure acquisition module, which is installed on the inner side of the push plate, and is used to monitor the contact state and pressure distribution between the push plate and the counting plate, and generate a position offset through the control module. The control module generates a pressure fluctuation coefficient; the staff manually inserts the counting plate into the inlet plate, and then pushes the counting plate into the inlet plate. During this process, the offset acquisition module monitors the initial insertion position and posture of the cell counting plate, and generates a position offset through the control module. The pressure acquisition module monitors the contact status and pressure distribution between the push plate and the counting plate, and generates a pressure fluctuation coefficient through the control module. The control module conducts a comprehensive analysis of the generated pressure fluctuation coefficient and position offset, generates an evaluation coefficient, and determines whether the adjustment mechanism needs to adjust the position of the counting plate. The evaluation coefficient is compared with the pre-set evaluation coefficient reference threshold, and the working status of the mechanism is adjusted according to the comparison result.

[0006] Preferably, the front end of the counter is fixedly connected to a docking frame located at the inlet, and a conveying trough docking with the inlet plate is provided in the docking frame, and motors are fixedly connected to both sides of the conveying trough, and the motor output shaft is fixedly connected to a connecting rod, and the free end of the connecting rod is connected to a plug rod that can move up and down, and a slot for inserting the plug rod is provided at the end of the counting plate; first lift the two plug rods upward, and then place the counting plate into the conveying trough, and then release the plug rod, the bottom end of the plug rod will be inserted into the slot, and then start the motor, the motor output shaft will drive the connecting rod to rotate, and then the counting plate is pushed into the inlet plate through the plug rod to realize the insertion of the counting plate, and vice versa, the counting plate can be pulled out, which can replace manual plugging and unplugging.

[0007] Preferably, a sliding connection is formed between the connecting rod and the insertion rod through a sliding groove, a spring is fixedly connected to the bottom inner wall of the sliding groove, and the top end of the spring is fixedly connected to the connecting rod; in this way, the insertion rod can move freely in the vertical direction.

[0008] Preferably, the top ends of the two insertion rods are jointly sleeved with a horizontal plate, the top of the horizontal plate is fixedly connected to a handle, and the top of the docking frame is provided with a sliding opening for the handle to pass through; the horizontal plate can be pulled by the handle, and then the two insertion rods can be lifted up at the same time through the horizontal plate, which makes the lifting process of the insertion rods more convenient.

[0009] Preferably, a photoelectric sensor aligned with the slot is installed at the bottom end of the insertion rod, and a limit switch is installed on one side of the conveying slot; the photoelectric sensor detects whether the insertion rod is aligned with the slot. When the insertion rod reaches the correct position, the photoelectric sensor triggers a signal to notify the control module to start the plugging and unplugging action, and detects whether the counting board is fully inserted or pulled out through the limit switch. When the counting board touches the limit switch, the sensor sends a signal to the control module to stop the motor action.

[0010] Preferably, the output end and input end of the offset acquisition module, the output end and input end of the pressure acquisition module, the output end and input end of the photoelectric sensor, and the output end and input end of the limit switch are electrically connected to the input end and output end of the control module respectively, and the output end of the control module is electrically connected to the input end of the electric push rod and the input end of the motor respectively.

[0011] Preferably, the control module controls the working state of the adjustment mechanism according to the comparison result in the following steps:

[0012] Real-time detection: The offset acquisition module monitors the initial insertion position and posture of the counting plate; the pressure acquisition module monitors the contact status and pressure distribution between the push plate and the counting plate;

[0013] Coefficient calculation: The control module calculates the pressure fluctuation coefficient, position offset and evaluation coefficient;

[0014] Dynamic adjustment: If :The control module increases the adjustment frequency of the push plate and improves the pressure feedback sensitivity to quickly correct the offset; if : Keep the current push plate motion parameters and only make fine adjustments.

[0015] Preferably, the logic for generating the position offset is:

[0016] S1. During the insertion of the counting plate, the offset acquisition module collects the lateral offset value between the center line of the counting plate and the center line of the inlet in real time at a fixed time interval Δt, and records it as , ,…, ;

[0017] Time window: The total time T from the beginning of the insertion of the counting slide to its complete position, with a total of t=T / Δt data points collected;

[0018] S2. Calculate the root mean square error. The calculation expression is:

[0019]

[0020] Where, is the lateral offset value at the mth time point; is the number of pressure sampling times within time T.

[0021] Preferably, the logic for generating the pressure fluctuation coefficient is:

[0022] S1. During the push plate adjustment process, the pressure acquisition module collects the contact pressure value of the push plate on the counting plate in real time at the same time interval Δt and records it as , ,…, ;

[0023] Time window: The total time T′ from the start of the push plate movement to the end of the adjustment, a total of k=T′ / Δt data points are collected;

[0024] S2. Calculate the standard deviation σP and amplify the fluctuation effect through the exponential function to generate the pressure fluctuation coefficient Pσ. The calculation expression is:

[0025]

[0026] Where, is the average pressure value.

[0027] Preferably, the control module performs a formula analysis according to the formula:

[0028]

[0029] Where, is the evaluation coefficient, and α and β are the preset weight coefficients of position offset and pressure fluctuation.

[0030] Compared with the prior art, the present invention provides an automatic cell counting device with the following advantages:

[0031] 1. The automatic cell counting device uses an offset acquisition module to monitor the initial insertion position and posture of the counting slide in real time. It also uses a pressure acquisition module to monitor the contact state and pressure distribution between the push plate and the counting slide. The control module then performs comprehensive analysis and dynamic adjustments. This dual feedback mechanism accurately corrects the position deviation of the counting slide, effectively reducing eccentric wear and ensuring that the counting slide is always in the optimal detection position, thereby improving cell counting accuracy.

[0032] 2. The automated plug-in / plug-out mechanism of the cell counter, consisting of a docking frame, motor, connecting rod, and inserting rod, replaces the traditional manual plug-in / plug-out method, greatly improving operational efficiency. The combination of photoelectric sensors and limit switches enables precise control of the plug-in / plug-out process, further enhancing the automation level of the equipment.

[0033] 3. Automated cell counting equipment collects and analyzes key parameters such as position offset and pressure fluctuation coefficient in real time, generating evaluation coefficients. By comparing these values ​​with preset reference thresholds, the counting chamber insertion process is monitored and intelligently adjusted. This data-driven control approach not only improves the reliability of counting results but also provides a powerful basis for subsequent quality control and equipment optimization. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 This is a schematic diagram of the overall structure of the automatic cell counting device provided in Example 1 of the present invention;

[0035] Figure 2 This is a schematic diagram of the internal structure of the automatic cell counting device proposed in the present invention;

[0036] Figure 3 This is a schematic diagram of the internal structure of the inlet guard plate of the automatic cell counting device proposed in the present invention;

[0037] Figure 4 This is a schematic diagram of the overall structure of the automatic cell counting device provided in Example 2 of the present invention;

[0038] Figure 5 This is a schematic diagram of the internal structure of the docking frame of the automatic cell counting device proposed in the present invention;

[0039] Figure 6 For the present invention Figure 5 A schematic diagram of the enlarged structure at point A;

[0040] Figure 7 This is a schematic diagram of the installation structure between the connecting rod and the inserting rod of the automatic cell counting device proposed by the present invention;

[0041] Figure 8 This is a system block diagram of the automatic cell counting device proposed in the present invention.

[0042] In the figure: 1. Counter; 2. Inlet guard plate; 3. Inlet plate; 4. Opening; 5. Electric push rod; 6. Push plate; 7. Offset acquisition module; 8. Pressure acquisition module; 9. Docking frame; 10. Conveyor trough; 11. Motor; 12. Connecting rod; 13. Counting plate; 14. Insert rod; 15. Notch; 16. Slide; 17. Spring; 18. Horizontal plate; 19. Handle; 20. Slide; 21. Limit switch. DETAILED DESCRIPTION

[0043] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0044] In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as limiting the present invention.

[0045] Reference Figures 1-8 In Example 1, an automatic cell counting device includes a counter 1, an inlet plate 3 is fixedly inserted into an inlet guard plate 2 at the inlet of the counter 1, and openings 4 are respectively opened on both sides of the inlet plate 3. An adjustment mechanism is provided in the inlet plate 3, and the adjustment mechanism includes a group of electric push rods 5 respectively fixedly connected to the inner walls of the two sides of the inlet guard plate 2, and the output shaft of the electric push rod 5 is fixedly connected to the push plate 6 located in the opening 4, and further includes:

[0046] The offset acquisition module 7 is installed on the inlet plate 3 near the inlet, and is used to monitor the initial insertion position and posture of the cell counting chamber in real time, and generate the position offset through the control module;

[0047] The pressure acquisition module 8 is installed on the inner side of the push plate 6 and is used to monitor the contact state and pressure distribution between the push plate 6 and the counting plate 13, and generate the pressure fluctuation coefficient through the control module;

[0048] It should be noted that the offset acquisition module 7 can be a photoelectric sensor or other device capable of monitoring the initial insertion position and posture of the cell counting plate in real time; the pressure acquisition module 8 can be a pressure sensor or other device capable of monitoring the contact state and pressure distribution between the push plate 6 and the counting plate 13 in real time; the control module is an embedded controller (such as the STM32 series), which is integrated on the circuit board of the counter 1 and integrates a data fusion algorithm. Therefore, the offset acquisition module 7, the pressure acquisition module 8 and the control module are not specifically limited here and can be selected according to actual needs;

[0049] During use, the staff manually inserts the counting plate 13 into the inlet plate 3, and then pushes the counting plate 13 into the inlet plate 3. During this process, the offset acquisition module 7 monitors the initial insertion position and posture of the cell counting plate, and generates a position offset through the control module. The pressure acquisition module 8 monitors the contact state and pressure distribution between the push plate 6 and the counting plate 13, and generates a pressure fluctuation coefficient through the control module. The control module comprehensively analyzes the generated pressure fluctuation coefficient and position offset, generates an evaluation coefficient, and determines whether the adjustment mechanism needs to adjust the position of the counting plate 13. The position adjustment method is that the electric push rod 5 drives the push plate 6 according to the instruction of the control module, fine-tunes the position of the counting plate 13 by applying lateral pressure, compares the evaluation coefficient with the pre-set evaluation coefficient reference threshold, and adjusts the working state of the mechanism according to the comparison result.

[0050] In Example 2, the front end of the counter 1 is fixedly connected to a docking frame 9 located at the inlet, and a conveying trough 10 is provided in the docking frame 9 for docking with the inlet plate 3. A motor 11 is fixedly connected to both sides of the conveying trough 10. The output shaft of the motor 11 is fixedly connected to a connecting rod 12. The free end of the connecting rod 12 is connected to an insertion rod 14 that can move up and down. A notch 15 is provided at the end of the counting plate 13 for inserting the insertion rod 14.

[0051] When in use, first lift the two insertion rods 14 upwards, then place the counting board 13 into the conveying trough 10, then loosen the insertion rod 14, the bottom end of the insertion rod 14 will be inserted into the slot 15, then start the motor 11, the output shaft of the motor 11 will drive the connecting rod 12 to rotate, and then push the counting board 13 into the inlet plate 3 through the insertion rod 14 to realize the insertion of the counting board 13. Otherwise, the counting board 13 can be pulled out, which can replace manual insertion and removal.

[0052] The connecting rod 12 and the inserting rod 14 are slidably connected via a sliding groove 16. A spring 17 is fixedly connected to the bottom inner wall of the sliding groove 16. The top end of the spring 17 is fixedly connected to the connecting rod 12.

[0053] When in use, this allows the insertion rod 14 to move freely in the vertical direction.

[0054] Furthermore, the top ends of the two insertion rods 14 are sleeved with a horizontal plate 18, the top of the horizontal plate 18 is fixedly connected with a handle 19, and the top of the docking frame 9 is provided with a sliding opening 20 for the handle 19 to pass through;

[0055] When in use, the handle 19 can be used to pull the transverse plate 18, and then the two insertion rods 14 can be lifted upward at the same time through the transverse plate 18, which makes the lifting process of the insertion rods 14 more convenient.

[0056] Furthermore, a photoelectric sensor aligned with the notch 15 is installed at the bottom end of the insertion rod 14, and a limit switch 21 is installed on one side of the conveying trough 10;

[0057] During use, the photoelectric sensor detects whether the insertion rod 14 is aligned with the slot 15. When the insertion rod 14 reaches the correct position, the photoelectric sensor triggers a signal to notify the control module to start the plugging and unplugging action, and detects whether the counting board 13 is fully inserted or unplugged through the limit switch 21. When the counting board 13 touches the limit switch 21, the sensor sends a signal to the control module to stop the motor 11.

[0058] Among them, the output end and input end of the offset acquisition module 7, the output end and input end of the pressure acquisition module 8, the output end and input end of the photoelectric sensor, and the output end and input end of the limit switch 21 are electrically connected to the input end and output end of the control module respectively, and the output end of the control module is electrically connected to the input end of the electric push rod 5 and the input end of the motor 11 respectively.

[0059] In another embodiment, the offset acquisition module 7, the pressure acquisition module 8, and the control module cooperate to generate a pressure fluctuation coefficient through the control module. The control module comprehensively analyzes the generated pressure fluctuation coefficient and the position offset to generate an evaluation coefficient, and determines whether the adjustment mechanism needs to adjust the position of the counting plate 13. The evaluation coefficient is compared with a preset evaluation coefficient reference threshold, and the working state of the mechanism is adjusted according to the comparison result. The specific execution steps are as follows:

[0060] Real-time detection: the offset acquisition module 7 monitors the initial insertion position and posture of the counting plate 13; the pressure acquisition module 8 monitors the contact state and pressure distribution between the push plate 6 and the counting plate 13;

[0061] Coefficient calculation: The control module calculates the pressure fluctuation coefficient, position offset and evaluation coefficient;

[0062] The logic for generating the position offset is:

[0063] S1. During the insertion of the counting plate 13, the offset acquisition module 7 acquires the lateral offset value between the center line of the counting plate 13 and the center line of the inlet in real time at a fixed time interval Δt, and records it as , ,…, ;

[0064] Time window: the total time T from the insertion of the counting plate 13 to its complete position, with a total of t=T / Δt data points collected;

[0065] S2. Calculate the root mean square error. The calculation expression is:

[0066]

[0067] Where, is the lateral offset value at the mth time point; is the number of pressure sampling times within time T.

[0068] Among them, the generation logic of the pressure fluctuation coefficient is:

[0069] S1. During the adjustment of the push plate 6, the pressure acquisition module 8 collects the contact pressure value of the push plate 6 on the counting plate 13 in real time at the same time interval Δt, and records it as , ,…, ;

[0070] Time window: the total time T′ from the start of the push plate 6 to the end of the adjustment, a total of k=T′ / Δt data points are collected;

[0071] S2. Calculate the standard deviation σP and amplify the fluctuation effect through the exponential function to generate the pressure fluctuation coefficient Pσ. The calculation expression is:

[0072]

[0073] Where, is the average pressure value.

[0074] Among them, the control module is used for formula analysis, according to the formula:

[0075]

[0076] Where, is the evaluation coefficient, and α and β are the preset weight coefficients of position offset and pressure fluctuation.

[0077] Dynamic adjustment: If : The control module increases the adjustment frequency of the push plate 6 and improves the pressure feedback sensitivity to quickly correct the offset; if :Maintain the current push plate 6 motion parameters and only make fine adjustments, where, To set the threshold.

[0078] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. An automatic cell counting device comprising a cell counting instrument (1), characterized in that: An inlet plate (3) is fixedly inserted into the inlet guard plate (2) at the inlet of the counter (1), and openings (4) are respectively opened on both sides of the inlet plate (3). An adjustment mechanism is provided in the inlet plate (3), and the adjustment mechanism includes a group of electric push rods (5) respectively fixedly connected to the inner walls of both sides of the inlet guard plate (2), and the output shaft of the electric push rod (5) is fixedly connected to the push plate (6) located in the opening (4), and further includes: An offset acquisition module (7) is installed on the inlet plate (3) near the inlet, and is used to monitor the initial insertion position and posture of the cell counting plate in real time, and generate a position offset through the control module; A pressure acquisition module (8) is installed on the inner side of the push plate (6) and is used to monitor the contact state and pressure distribution between the push plate (6) and the counting plate (13), and to generate a pressure fluctuation coefficient through the control module; The generated pressure fluctuation coefficient and position offset are comprehensively analyzed by the control module to generate an evaluation coefficient, which is compared with a pre-set evaluation coefficient reference threshold, and the working state of the adjustment mechanism is controlled according to the comparison result.

2. The automatic cell counting device according to claim 1, characterized in that: The front end of the counter (1) is fixedly connected to a docking frame (9) located at the inlet, and a conveying trough (10) is provided in the docking frame (9) and is docked with the inlet plate (3). Motors (11) are fixedly connected to both sides of the conveying trough (10), and the output shaft of the motor (11) is fixedly connected to a connecting rod (12). The free end of the connecting rod (12) is connected to an insertion rod (14) that can move up and down, and a notch (15) for inserting the insertion rod (14) is provided at the end of the counting plate (13).

3. The automatic cell counting device according to claim 2, characterized in that: The connecting rod (12) and the inserting rod (14) are slidably connected via a sliding groove (16); a spring (17) is fixedly connected to the bottom inner wall of the sliding groove (16); and the top end of the spring (17) is fixedly connected to the connecting rod (12).

4. The automatic cell counting device according to claim 2, characterized in that: The top ends of the two insertion rods (14) are sleeved with a transverse plate (18), the top of the transverse plate (18) is fixedly connected with a handle (19), and the top of the docking frame (9) is provided with a sliding opening (20) for the handle (19) to pass through.

5. The automatic cell counting device according to claim 2, characterized in that: A photoelectric sensor aligned with the notch (15) is installed at the bottom end of the insertion rod (14), and a limit switch (21) is installed on one side of the conveying trough (10).

6. The automatic cell counting device according to claim 5, characterized in that: The output end and input end of the offset acquisition module (7), the output end and input end of the pressure acquisition module (8), the output end and input end of the photoelectric sensor, and the output end and input end of the limit switch (21) are electrically connected to the input end and output end of the control module, respectively. The output end of the control module is electrically connected to the input end of the electric push rod (5) and the input end of the motor (11).

7. The automatic cell counting device according to claim 1, characterized in that: The control module controls the working state of the adjustment mechanism according to the comparison result in the following steps: Real-time detection: the offset acquisition module (7) monitors the initial insertion position and posture of the counting plate (13); the pressure acquisition module (8) monitors the contact state and pressure distribution between the push plate (6) and the counting plate (13); Coefficient calculation: The control module calculates the pressure fluctuation coefficient, position offset and evaluation coefficient; Dynamic adjustment: If :The control module increases the adjustment frequency of the push plate (6) and improves the pressure feedback sensitivity to quickly correct the offset; if :Keep the current push plate (6) motion parameters and only make fine adjustments, To set the threshold.

8. The automatic cell counting device according to claim 1, characterized in that: The logic for generating the position offset is: S1. During the insertion of the counting plate (13), the offset acquisition module (7) acquires the lateral offset value between the center line of the counting plate (13) and the center line of the inlet in real time at a fixed time interval Δt and records it as , ,…, ; Time window: the total time T from the insertion of the counting plate (13) to its complete position, with a total of t = T / Δt data points collected; S2. Calculate the root mean square error. The calculation expression is: Where, is the lateral offset value at the mth time point; is the number of pressure sampling times within time T.

9. The automatic cell counting device according to claim 8, characterized in that: The generation logic of the pressure fluctuation coefficient is: S1. During the adjustment process of the push plate (6), the pressure acquisition module (8) collects the contact pressure value of the push plate (6) on the counting plate (13) in real time at the same time interval Δt and records it as , ,…, ; Time window: the total time T′ from the start of the push plate (6) to the end of the adjustment, with a total of k = T′ / Δt data points collected; S2. Calculate the standard deviation σP and amplify the fluctuation effect through the exponential function to generate the pressure fluctuation coefficient Pσ. The calculation expression is: Where, is the average pressure value.

10. The automatic cell counting device according to claim 9, characterized in that: Through the control module, a formula analysis is performed according to the formula: Where, is the evaluation coefficient, and α and β are the preset weight coefficients of position offset and pressure fluctuation.

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