Application of alhagi sparsifolia extract in external preparation for skin and preparation method of alhagi sparsifolia extract

Abstract

The invention discloses an application of an alhagi sparsifolia extract in an external preparation for fading red skin, and discloses two preparation methods for preparing the alhagi sparsifolia extract. The alhagi sparsifolia extract provided by the invention can relieve skin redness, has a remarkable red fading effect, and can be used in an external preparation for fading red skin. In addition, the alhagi sparsifolia extract provided by the invention has the effect of inhibiting melanogenesis, and the alhagi sparsifolia extract provided by the invention can also be applied to skin whitening external preparations, skin brightening external preparations and skin melanin improving external preparations.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, in particular to application of a camel thorn extract in a skin external preparation and a preparation method of the camel thorn extract. Background Art

[0002] The skin primarily consists of the epidermis and dermis. The outermost layer of the epidermis is primarily composed of the sebaceous membrane and the stratum corneum. The sebaceous membrane is a transparent, slightly acidic film on the surface of the skin, primarily formed by a fusion of oil secreted by sebaceous glands and water secreted by sweat glands. The stratum corneum is a complex of proteins and lipids. Within the extracellular spaces of the stratum corneum, a lamellar membrane formed by highly hydrophobic lipids surrounds the stratum corneum. Redness is a symptom of skin that becomes red in a specific area, typically due to vasodilation and congestion. The blood vessels that cause redness are primarily located in the dermis. The dermis is the middle layer of the skin, composed of collagen fibers, elastic fibers, and blood vessels. When blood vessels dilate, blood flow to the area increases, causing the skin to appear red.

[0003] Camel thorn (Alhagi camelorum Fisch.) is a semishrubby herbaceous plant of the genus Alhagi in the Leguminosae family. Modern medicine has conducted extensive research on its extracts, leading to the development of new medical applications, such as preventing glaucoma and aggressive lesions of the substantia nigra (a key contributor to Parkinson's disease), lowering intraocular pressure, preventing and treating colon, stomach, liver, and oral cancers, and preventing and treating osteoporosis.

[0004] Patent RU2402345C2 discloses the application of camel thorn extract in the fields of medicine and skin topical preparations. Animal experiments have verified that oral administration of camel thorn extract can reduce the total cholesterol and triglyceride levels in animal serum and organs. Oral camel thorn extract has antioxidant and anti-inflammatory effects. Sunflower oil containing 5% camel thorn extract can promote the healing of burned skin when applied; skin biopsy experiments have confirmed that camel thorn can promote collagen synthesis.

[0005] As is well known to those skilled in the art, oral efficacy of an ingredient does not necessarily imply that topical administration will have the same or different efficacy based on its oral efficacy. For example, amoxicillin is a common clinical anti-inflammatory drug, primarily administered orally, and primarily works through intestinal absorption and distribution. However, topical use of amoxicillin is prohibited due to its potential to cause allergic reactions. Therefore, the efficacy of oral administration of camel thorn extract disclosed in the aforementioned patent RU2402345C2 does not necessarily imply that topical administration of the extract will have the same efficacy.

[0006] In summary, the prior art does not involve the application of camel thorn extract in the field of redness-reducing skin topical preparations and skin whitening topical preparations. Summary of the Invention

[0007] The purpose of this application is to develop new uses for camel thorn.

[0008] The first aspect of the present invention is to develop the application of camel thorn extract in skin external preparations, especially as the sole or one of the redness-reducing ingredients in redness-reducing skin external preparations.

[0009] Preferably, the recommended addition percentage by mass of the camel thorn extract in the external preparation for de-erythema is 0.01-2%.

[0010] Specifically, the anti-redness skin external preparation is a skin external preparation used to relieve skin redness.

[0011] Furthermore, the erythema-reducing external preparation for skin is a dermatological preparation for skin use that is used to relieve skin redness caused by external stimulation; or the erythema-reducing external preparation for skin use is a dermatological preparation for skin use that is used to relieve skin redness caused by internal stimulation.

[0012] Furthermore, the skin external preparation is:

[0013] Topical skin preparations for soothing redness caused by capsaicin irritation;

[0014] Or it can be used as a topical preparation for soothing redness caused by irritation caused by alcohol.

[0015] Or as a topical skin preparation for soothing redness caused by histamine irritation.

[0016] Histamine is an organic nitrogen compound produced from histidine by the action of decarboxylase. Many tissues, particularly mast cells in the skin, lungs, and intestinal mucosa, contain large amounts of histamine. Histamine is released in response to tissue damage, inflammation, or allergic reactions. Histamine has a potent vasodilatory effect and can increase the permeability of capillary and venular walls, allowing plasma to leak into tissues and causing localized tissue edema. Therefore, histamine stimulation can be externally introduced or internally produced.

[0017] The second aspect of the present invention is to develop a new application of camel thorn extract in skin external preparations, especially as the only or one of the whitening ingredients in skin whitening external preparations.

[0018] Furthermore, the skin whitening external preparation is:

[0019] topical preparations for skin use to inhibit melanin production;

[0020] or a topical skin preparation for improving skin melanin;

[0021] or topical preparations for skin lightening;

[0022] Or used as external skin preparation for removing freckles.

[0023] Specifically, the recommended addition percentage by mass of the camel thorn extract in the skin whitening external preparation is 0.01-2%; preferably, the percentage by mass is 0.01-0.2%; further, it is 0.1-0.2%.

[0024] The third aspect of the present invention provides a method for preparing the camel thorn extract, comprising the following steps:

[0025] Step A1: After crushing camel thorn, adding purified water, heating and refluxing for extraction, filtering, and collecting the filtrate to obtain a crude camel thorn extract;

[0026] Step A2: The crude extract of camel thorn is separated by macroporous resin column chromatography, and gradient eluted with purified water and ethanol aqueous solution in sequence. The ethanol aqueous solution is collected, concentrated and dried to obtain the camel thorn extract.

[0027] Specifically:

[0028] In step A1, the mass volume ratio of the camel thorn to the purified water is 1:5 to 1:30 g / mL; and the heating reflux time is 0.5 to 4 hours;

[0029] In step A2, the loading amount of the macroporous resin / the amount of camel thorn drug added is 0.5 to 2; the column volume:the loading amount of the macroporous resin is 1:1 BV / g, the ratio of the macroporous adsorption resin column diameter to the column height is 1:5 to 1:20; the loading rate of the macroporous resin column chromatography separation is 0.5 to 4 BV / h;

[0030] In step A2, during gradient elution, 2 to 10 BV of purified water and 2 to 10 BV of 80% ethanol aqueous solution are sequentially used for gradient elution, and the flow rate of the gradient elution is 1 to 1.5 BV / h;

[0031] The fourth aspect of the present invention provides a camel thorn extract prepared by the above method.

[0032] A fifth aspect of the present invention provides another method for preparing the camel thorn extract, comprising the following steps:

[0033] Step B1: After crushing camel spur, adding ethanol, heating under reflux for extraction, filtering, collecting the filtrate, concentrating and drying to obtain a camel spur pre-extract;

[0034] Step B2: adding purified water to the camel thorn extract and stirring for a period of time to obtain a camel thorn crude extract;

[0035] Step B3: The crude extract of camel thorn is separated by macroporous resin column chromatography, and gradient eluted with purified water and ethanol aqueous solution in sequence, the ethanol aqueous solution is collected, and concentrated and dried to obtain the camel thorn extract.

[0036] The mass volume ratio of the camel thorn to the ethanol is 1:5 to 1:30 g / mL;

[0037] The heating reflux time is 0.5 to 4 hours;

[0038] The mass volume ratio of the camel thorn extract to the purified water is 1:5 to 1:30 g / mL;

[0039] The loading amount of the macroporous adsorption resin / the dosage of camel thorn is 0.5 to 2, the ratio of the macroporous adsorption resin column diameter to the column height is 1:5 to 1:20; the loading speed of the macroporous resin column chromatography separation is 0.5 to 4 BV / h;

[0040] In the gradient elution, 2 to 10 BV of purified water and 2 to 10 BV of an 80% ethanol aqueous solution are sequentially used for gradient elution, and the flow rate of the gradient elution is 1 to 1.5 BV / h;

[0041] The sixth aspect of the present invention provides an camel thorn extract prepared by the above method.

[0042] The beneficial effects achieved by the present invention are as follows: the present invention discloses two new camel thorn extraction methods, which are verified by skin redness model experiments to show that the camel thorn extracts obtained by the two methods can soothe skin redness and have a significant redness-reducing effect. The camel thorn extracts provided by the present invention can be used in redness-reducing skin external preparations.

[0043] In addition, zebrafish experiments have verified that the camel thorn extract provided by the present invention has the effect of inhibiting melanin production. Therefore, the camel thorn extract of the present invention can also be applied to skin whitening topical preparations, skin topical preparations for improving skin melanin, skin topical preparations for inhibiting melanin production, skin brightening topical preparations, or freckle-removing topical preparations. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0045] Figure 1 Antera 3D images of the capsaicin-induced skin redness model in the embodiment - soothing human efficacy test (image acquisition instrument used: multifunctional 3D skin imaging analyzer / Antera HC1023Q);

[0046] Figure 2 This is the Antera 3D image of the histamine-stimulated skin redness model - soothing human efficacy test in the examples;

[0047] Figure 3 This is a graph showing the melanin inhibitory effects of camel thorn extract A and camel thorn extract B evaluated by zebrafish skin whiteness in the Examples. DETAILED DESCRIPTION

[0048] The preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0049] Example 1

[0050] This embodiment provides a camel thorn extract, comprising the following steps:

[0051] Take 200g of camel thorn medicinal material, crush it, add 2000mL of purified water, heat and reflux extraction for 2h, filter, collect the filtrate, and apply AB-8 macroporous adsorption resin for separation once (resin loading amount is 200g, column volume is 200mL / BV, and the ratio of macroporous adsorption resin column diameter to column height is 1:10) at a loading speed of 1BV / h; after the loading is completed, gradient elution is performed with 5BV of purified water and 5BV of 80% ethanol aqueous solution by volume, and the flow rate of the gradient elution is 1-1.5BV / h. The 80% ethanol aqueous solution is collected and concentrated and dried to obtain 1.8g of camel thorn extract A.

[0052] Example 2

[0053] This embodiment provides a camel thorn extract, comprising the following steps:

[0054] Take 200g of camel thorn medicinal material, crush it, add 2000mL of ethanol, heat and reflux extraction for 2h, filter, collect the filtrate, concentrate and dry the filtrate to obtain 32g of camel thorn pre-extract, add 200mL of purified water and stir for 2h, then apply AB-8 macroporous adsorption resin for separation once (resin loading amount is 200g, column volume is 200mL / BV, and the ratio of macroporous adsorption resin column diameter to column height is 1:10), and the loading speed is 1BV / h; after the loading is completed, gradient elution is performed with 5BV of purified water and 5BV of 80% ethanol aqueous solution by volume, and the flow rate of the gradient elution is 1-1.5BV / h. Collect the 80% ethanol aqueous solution, concentrate and dry to obtain 2.1g of camel thorn extract B.

[0055] Example 3

[0056] This example demonstrates the efficacy of the camel thorn extract provided in Examples 1-2 in soothing and fading redness through a capsaicin-stimulated skin redness model. Specifically, the following are included:

[0057] 1. Sample preparation:

[0058] Sample preparation: 1 g of camel thorn extract A and camel thorn extract B provided in Example 1 and Example 2 were weighed respectively, and dissolved in 100 mL of butylene glycol to prepare a camel thorn extract aqueous solution with a mass concentration of 1%.

[0059] 2. Based on the inclusion and exclusion criteria, professional researchers inquired, observed and examined the subjects' previous cosmetic use history, skin sensitivity, physical health and skin condition of the test site. Finally, 45 skin subjects who met the inclusion and exclusion criteria were selected and divided into three groups on average. The subjects were treated with 1% camel thorn extract aqueous solution A, 1% camel thorn extract aqueous solution B and placebo butylene glycol, respectively. The method of use was to apply equal amounts of each test substance to the arm and use the multifunctional 3D skin imaging analyzer Antera HC1023Q (Miravex) collected and analyzed redness images of each test site to obtain skin redness parameters, and the average value was recorded.

[0060] 3. Establishment of Capsaicin Stimulation-Skin Barrier Damage Model

[0061] Test environment requirements: temperature 20-22°C, relative humidity 40-60% RH;

[0062] Measurement time points: Arm clinical tests were performed 4 times in total: after modeling (T0), 10 minutes after using the product (T10min), 30 minutes after using the product (T30min), and 1 hour after using the product (T1h).

[0063] 3.1 After resting for 30 minutes in a constant temperature and humidity environment, three 9cm holes were selected on the flexion side of the left and right forearms of the subjects. 2 (3cm×3cm) area and marked with a marker. The two adjacent test areas on the same arm should be at least 1cm apart (the measurement site should remain consistent at each time point of the study);

[0064] 3.2 Wipe the test area with fiber paper, then soak a 3cm×3cm wet compress with 0.4g of 0.05% capsaicin solution in each test area, apply the wet compress for 10 minutes, and then remove it. After removing it, use another piece of wet compress soaked in warm water for 5 minutes and then remove it. After waiting for 10 minutes, when the test area shows visible redness, the capsaicin stimulation-skin barrier damage model is established.

[0065] 4. Verification of Camel Thorn Extract’s Efficacy in Soothing and Reducing Redness

[0066] 4.1 Using the multifunctional 3D skin imaging analyzer Antera HC1023Q (Miravex) collected T0 images of each test site that was not smeared with the test product and analyzed them to obtain the skin redness parameters at T0;

[0067] 4.2 Use a pipette / syringe to aspirate the test sample and measure 18mg ± 0.9mg of the product using an analytical balance. Wear a disposable finger cot and apply the test sample evenly to the corresponding test area according to the randomization table. Allow the skin to completely absorb the test sample. Use butylene glycol as the base for the placebo control area.

[0068] 4.3 Use Antera 3D to collect images and analyze skin redness parameters 10 minutes, 30 minutes, and 1 hour after using the product. Keep the test area on the forearm dry and avoid touching or washing it while waiting for the test. During the test, the subject must not use any cosmetics, medications, or health products that may affect the results.

[0069] The test results are as follows Figure 1 and as shown in Table 1:

[0070] Table 1 Redness parameters of Antera 3D stimulated by capsaicin

[0071]

[0072] The results of the soothing efficacy test - capsaicin-stimulated redness model test show that since each raw material contains an alcohol matrix, the alcohol matrix has a certain degree of irritation to the skin after capsaicin modeling. The butylene glycol alcohol matrix without the addition of extracts was used as a placebo control example:

[0073] After 10 minutes of application, only the camel thorn extract provided in Example 2 showed a certain redness-reducing effect on the skin reddened by capsaicin stimulation;

[0074] After 30 minutes of application, the camel thorn extract provided in Example 2 showed a certain redness-reducing effect on the skin that was red after capsaicin stimulation;

[0075] After 1 hour of application, the camel thorn extracts provided in Examples 1-2 showed a certain redness-reducing effect on the skin reddened by capsaicin stimulation, and the camel thorn extract B provided in Example 2 had a significant redness-reducing effect.

[0076] Example 4

[0077] This example demonstrates the efficacy of the camel thorn extract prepared in Examples 1-2 in soothing and fading redness using a histamine-stimulated skin redness model. Specifically, the following are included:

[0078] 1. Sample preparation:

[0079] Sample preparation: 1 g of the camel thorn extract A prepared in Examples 1-2 was weighed and dissolved in 100 mL of butanediol to prepare an aqueous solution of the camel thorn extract with a mass concentration of 1%.

[0080] 2. Based on the inclusion and exclusion criteria, professional researchers inquired, observed and examined the subjects' previous cosmetic use history, skin sensitivity, physical health and skin condition of the test site. Finally, 45 skin subjects who met the inclusion and exclusion criteria were selected and divided into three groups on average. The subjects were treated with 1% camel thorn extract aqueous solution A, 1% camel thorn extract aqueous solution B and placebo butylene glycol, respectively. The method of use was to apply equal amounts of each test substance to the arm and use the multifunctional 3D skin imaging analyzer Antera HC1023Q (Miravex) collected and analyzed redness images of each test site to obtain skin redness parameters, and the average value was recorded.

[0081] 3. Establishment of Histamine Stimulation-Skin Barrier Damage Model

[0082] Test environment requirements: temperature 20-22°C, relative humidity 40-60% RH;

[0083] Measurement time points: Arm clinical tests were performed 4 times in total: after modeling (T0), 10 minutes after using the product (T10min), 30 minutes after using the product (T30min), and 1 hour after using the product (T1h).

[0084] 3.1 After resting for 30 minutes in a constant temperature and humidity environment, three 9cm holes were selected on the flexion side of the left and right forearms of the subjects. 2 (3cm×3cm) area and marked with a marker. The two adjacent test areas on the same arm should be at least 1cm apart (the measurement site should remain consistent at each time point of the study);

[0085] 3.2 Wipe the test area with fiber paper. Then, soak a 1.5 cm × 1.5 cm wet compress with 0.2 g of 1% histamine solution in each test area. Place it in the center of each test area for 15 minutes and then remove it. After waiting for another 15 minutes, if obvious redness and itching reaction appears on the skin, the histamine stimulation-skin barrier damage model is established.

[0086] 4. Antera 3D redness parameter verifies the soothing and redness-reducing effect of camel thorn extract

[0087] 4.1 Using the multifunctional 3D skin imaging analyzer Antera HC1023Q (Miravex) collected T0 images of each test site that was not smeared with the test product and analyzed them to obtain the skin redness parameters at T0;

[0088] 4.2 Use a pipette / syringe to aspirate the test sample and measure 18mg ± 0.9mg of the product using an analytical balance. Wear a disposable finger cot and apply the test sample evenly to the corresponding test area according to the randomization table. Allow the skin to completely absorb the test sample. Use butylene glycol as the base for the placebo control area.

[0089] 4.3 After using the product, Antera 3D will be used to collect images and analyze them again at T30 minutes, T1 hour, and T5 hours to obtain skin redness parameters. During the waiting period, the subject's forearm test area should be kept dry and avoid touching or washing. During the trial, the subject should not use any cosmetics, medications, or health products that may affect the results.

[0090] The test results are as follows Figure 2 and as shown in Table 2:

[0091] Table 2 Histamine-stimulated Antera 3D redness parameters

[0092]

[0093] The results of the histamine stimulation-skin barrier damage model test show that since each raw material contains an alcohol matrix, butylene glycol is used as a placebo control:

[0094] After 30 minutes of use, the camel thorn extract and butylene glycol base provided in Examples 1-2 showed a certain redness-reducing effect on the skin reddened after histamine stimulation. The redness-reducing effect was as follows: Example 1 > Example 2 > butylene glycol base.

[0095] After 1 hour of use, the camel thorn extract provided in Examples 1-2 demonstrated a moderate redness-reducing effect on skin reddened by histamine stimulation, with Example 1 being superior to Example 2. The butylene glycol matrix exhibited no redness-reducing effect, and the irritation of the alcohol matrix to the skin gradually became apparent as the test time increased.

[0096] After 5 hours of use, the camel thorn extract and butylene glycol matrix provided in Examples 1-2 showed a certain redness-reducing effect on the skin reddened after histamine stimulation. The redness-reducing effect was as follows: Example 1 > Example 2 > butylene glycol matrix.

[0097] Example 5

[0098] Zebrafish camel thorn extract concentration toxicity test

[0099] Principle and method:

[0100] Zebrafish are currently one of the most widely used surrogate species in human health and ecological hazard assessments. Zebrafish share 87% genetic similarity with humans, making them the third most popular model organism after mice and rats. Zebrafish can exhibit mortality and developmental toxicity in response to environmental and chemical exposures. Therefore, zebrafish mortality and toxicity patterns can be used to determine the test concentration of a sample.

[0101] Experimental steps:

[0102] 1. Randomly select zebrafish and place them in a 6-well plate, with 15 zebrafish per well.

[0103] 2. Dissolve the sample in water to the test concentration and set up a normal control group (using deionized water). The volume of each well is 3 mL.

[0104] Incubate at 3.28°C in the dark for 45 h.

[0105] 4. Based on the mortality and toxicity of zebrafish, determine the toxicity of the test concentration to normal zebrafish.

[0106] The test results are shown in Table 3.

[0107] Table 3 Toxicity test results of zebrafish camel thorn extract detection concentration

[0108]

[0109]

[0110] Conclusion: Under the experimental conditions, 0.2% camel thorn extract A had no toxicity to normal zebrafish; 0.2% camel thorn extract B had no toxicity to normal zebrafish.

[0111] Example 6

[0112] Zebrafish whitening efficacy test

[0113] System and sample size:

[0114] Experimental system: Wild-type AB strain zebrafish.

[0115] Zebrafish age: 6 hours post fertilization (6hpf).

[0116] The sample size of each experimental group was 15 (N=10).

[0117] Adult fish rearing and breeding methods: in line with the requirements of the international AAALAC certification (certification number: 001458).

[0118] Principle and method:

[0119] Zebrafish are transparent during early development. Melanin begins to grow from the retinal epithelium around 24 hours into embryonic development. Pigment cells originate from neural crest cells, a group of cells that differentiate from the dorsal ectoderm. These cells then proliferate, migrate, and differentiate into melanocyte progenitors. Interventions during melanin formation can inhibit melanin production. The whiteness of zebrafish skin is used to evaluate the whitening properties of a product.

[0120] Experimental steps:

[0121] 1. Randomly select zebrafish and place them in a 6-well plate, with 15 zebrafish per well.

[0122] 2. Dissolve the sample in water and set up a normal control group at the same time. The volume of each well is 3mL.

[0123] Incubate at 3.28°C in the dark for 45 h.

[0124] 4. For each experimental group, 10 zebrafish were randomly selected and photographed under a dissecting microscope. Advanced image processing software was used to analyze and collect data. The melanin signal intensity (S) of the zebrafish head was analyzed and the formula was used to calculate and determine whether the sample had whitening effect.

[0125]

[0126] The test results are shown in Table 4 and Figure 3 : Camel thorn extract A and camel thorn extract B have whitening effect.

[0127] Table 4 Zebrafish whitening efficacy test results

[0128]

[0129] Application Example 1

[0130] A soothing serum containing the following components in the following percentages by weight:

[0131] Phase A: deionized water 87.73%, disodium EDTA 0.02%, glycerol 5%, butylene glycol 3%, glycereth-26 2%, hexylene glycol 0.5%, p-hydroxyacetophenone 0.5%, carbomer 0.15%;

[0132] Phase B: sodium hydroxide 0.04%;

[0133] Phase C: PEG-40 hydrogenated castor oil 0.05%, essential oil 0.01%;

[0134] Phase D: Camel thorn extract 1%.

[0135] The process is as follows:

[0136] Mix phase A evenly, heat to 75-80℃, homogenize at 3000rpm for 30s, keep warm for 30min, and then start cooling;

[0137] Cool down to 45℃, add phase B, phase C, and phase D in sequence and stir to dissolve evenly; cool down to 25℃ to obtain the soothing essence.

[0138] Application Example 2

[0139] A soothing facial cream comprising the following components in the following percentages by weight:

[0140] Phase A: deionized water 75.55%, disodium EDTA 0.02%, glycerol 5%, butylene glycol 3%, glycereth-26 2%, hexylene glycol 0.5%, p-hydroxyacetophenone 0.5%, carbomer 0.3%;

[0141] Phase B: Polyglyceryl-3 Methyl Glucose Distearate 3%, PEG-100 Stearate & Glyceryl Stearate 1%, Jojoba Oil 2%, Shea Butter 1%, Caprylic / Capric Triglyceride 5%;

[0142] Phase C: 0.12% sodium hydroxide;

[0143] Phase D: essential oil 0.01%, camel thorn extract 1%;

[0144] The process is as follows:

[0145] Mix phase A evenly, heat to 75-80℃, and keep warm for 20 minutes; mix phase B evenly, heat to 75-80℃, and keep warm for 10 minutes; add phase B to phase A, homogenize at 3000rpm for 5 minutes, keep warm for 20 minutes, then start cooling to 45℃, add phase C and phase D in turn, and stir to dissolve evenly; cool to 25℃, inspect and discharge after passing the test, and you will get the soothing facial cream.

[0146] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art will be able to modify the technical solutions described in the aforementioned embodiments or substitute equivalents for some of the technical features. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. Application of camel thorn extract in a skin external preparation, wherein the skin external preparation is a redness-reducing skin external preparation.

2. The use according to claim 1, characterized in that The mass percentage of camel thorn extract in the skin external preparation is 0.01% to 2%.

3. The use according to claim 1 or 2, characterized in that The topical preparation for de-erythematous skin is: Topical skin preparations for soothing redness caused by capsaicin irritation; Or it can be used as a topical preparation for soothing redness caused by irritation caused by alcohol. Or as a topical skin preparation for soothing redness caused by histamine irritation.

4. A method for preparing an extract of camel thorn, characterized in that: The following steps are involved: Step A1: After crushing camel thorn, adding solvent for extraction to obtain a crude camel thorn extract; Step A2: The crude extract of camel thorn is separated by macroporous resin column chromatography, and gradient eluted with purified water and ethanol aqueous solution in sequence. The ethanol aqueous solution is collected, concentrated and dried to obtain the camel thorn extract.

5. The preparation method according to claim 4, wherein In step A1, the solvent is purified water, and the method of adding the solvent for extraction is: heating under reflux for extraction, filtering, and collecting the filtrate to obtain a crude extract of camel thorn.

6. The preparation method according to claim 4, wherein In step A1, the solvent is ethanol, and the method of adding the solvent for extraction is: heating and refluxing extraction, filtering, collecting the filtrate, concentrating, and drying to obtain a camel thorn pre-extract; the camel thorn pre-extract is added with purified water and stirred for a period of time to obtain a camel thorn crude extract.

7. The preparation method according to claim 5 or 6, characterized in that In step A1, the mass volume ratio of camel thorn to solvent is 1:5 to 1:30 g / mL; and the heating reflux time is 0.5 to 4 h.

8. The preparation method according to claim 4, wherein In step A2, the loading amount of the macroporous resin / the dosage of camel thorn is 0.5-2; the ratio of the macroporous adsorption resin column diameter to the column height is 1:5-1:20; and the loading speed of the macroporous resin column chromatography separation is 0.5-4 BV / h.

9. The preparation method according to claim 4, wherein In step A2, during gradient elution, 2 to 10 BV of purified water and 2 to 10 BV of 80% ethanol aqueous solution are sequentially used for gradient elution, and the flow rate of the gradient elution is 1 to 1.5 BV / h.

Images