A composition for refreshing the mind and relieving fatigue

Abstract

The invention relates to the technical field of plant essential oils and discloses a composition for refreshing the mind and alleviating drowsiness. The composition comprises the following steps: synthesizing an isobutylene-containing solid lipid, an n-hexane-containing solid lipid and an adhesion-functional solid lipid using stearic acid as a basic raw material; pre-assembling a composite solid lipid consisting of the isobutylene-containing solid lipid, the n-hexane-containing solid lipid, the adhesion-functional solid lipid and glyceryl monostearate and a plant essential oil component having the functions of refreshing the mind and alleviating drowsiness and containing an isobutylene group, an n-hexane skeleton or a hydroxyl group based on adsorption or hydrogen bond adhesion between similar structures to obtain a lipid-essential oil mixture; and emulsifying the lipid-essential oil mixture with an aqueous phase through a high-pressure homogenization method to obtain the composition for refreshing the mind and alleviating drowsiness, wherein the composition exhibits excellent sustained-release performance, can effectively prolong the release time of the plant essential oil component and improve the stability of the plant essential oil.

Description

Technical Field

[0001] The invention relates to the technical field of plant essential oils, in particular to a composition for refreshing the mind and relieving fatigue. Background Art

[0002] Natural plant essential oils containing active ingredients such as terpenes, alcohols, and phenols can affect the secretion of neurotransmitters or the expression of receptors in the central nervous system of the brain through olfactory stimulation or physiological effects, showing the effects of refreshing the mind, resisting fatigue, and improving cognition. Among them, menthol ( ) and borneol ( ) is a monoterpene alcohol compound, which is an oxygen-containing derivative of monoterpenes extracted from natural plant essential oils. Lime oil (the main component is limonene ), lavender oil (main component is linalool and linalyl acetate ), sweet basil oil (main component is linalool ), Geranium oil (main component is citronellol and linalool ) and eucalyptus globulus leaf oil (main component is 1,8-cineole and α-pinene ) is a natural plant essential oil containing monoterpenes as active ingredients, patchouli oil (the main component is patchouli alcohol ) is a natural plant essential oil containing sesquiterpenes as active ingredients, clove oil (main component is eugenol ) are natural plant essential oils containing phenolic active ingredients. They can all play a role in refreshing the mind and relieving fatigue through non-invasive aromatherapy (such as aromatherapy, inhalation, etc.).

[0003] Although plant essential oils have good anti-fatigue functions, they are highly volatile and have poor stability. They are easily affected by the external environment during storage and use, resulting in large amounts of volatilization, which greatly limits their application in actual production and life.

[0004] Currently, nanoencapsulation technology is commonly used to encapsulate essential oils in nanoscale carriers, protecting their active ingredients and thus expanding the application range of plant essential oils. Nanostructured lipid carriers are a lipid-based nanoencapsulation technology. Compared with other nanoencapsulation technologies such as nanoemulsions and microemulsions, they offer higher stability and better biocompatibility. There are reports of using nanostructured lipid carriers to enhance the stability of essential oils.

[0005] Nanostructured lipid carriers are composed of lipid phase (solid lipids and liquid lipids), aqueous phase and surfactants. The compatibility of solid lipids with essential oil components directly affects the stability of the carrier, encapsulation efficiency and essential oil release performance. If the solid lipids have poor compatibility with essential oils, it may lead to phase separation and precipitation, lipid crystallization rearrangement or essential oil leakage. When the solid lipids and essential oils have excellent compatibility, the essential oils can also act as excipients by imitating the behavior of liquid lipids. In this way, the essential oils have the dual role of active ingredients and functional excipients.

[0006] The study found that dopamine molecules with a catechol structure have strong adhesion and can be used to modify the surface of nanostructured lipid carriers, improve carrier stability, prevent aggregation or degradation, and enhance the encapsulation efficiency of nanostructured lipid carriers for essential oils. Summary of the Invention

[0007] The present invention provides a composition for refreshing the mind and relieving fatigue. The composition uses a plant essential oil component with good anti-fatigue effect as the refreshing and fatigue-relieving functional component. The plant essential oil component is encapsulated by using independently developed composite solid lipids through nanostructured lipid carrier technology, thereby protecting the active functional ingredients and improving stability.

[0008] A composition for refreshing the mind and alleviating drowsiness, the composition being preassembled from a composite solid lipid and a plant essential oil component containing an isobutylene group, an n-hexane skeleton, or a hydroxyl group, which has refreshing and drowsiness-relieving functions, based on adsorption or hydrogen bond adhesion between similar structures, and then emulsified with an aqueous phase by a high-pressure homogenization method;

[0009] The composite solid lipid is composed of an isobutylene-containing solid lipid, a hexane-containing solid lipid, an adhesion-functional solid lipid, and glyceryl monostearate.

[0010] Preferably, the preparation method of the isobutylene-containing solid lipid is: an esterification reaction occurs between the carboxyl functional group of stearic acid and the hydroxyl functional group of citronellol to generate the isobutylene-containing solid lipid.

[0011] Preferably, the preparation method of the solid lipid containing a normal hexane skeleton is: generating the solid lipid containing a normal hexane skeleton by an esterification reaction between the carboxyl functional group of stearic acid and the hydroxyl functional group of natural menthol.

[0012] Preferably, the preparation method of the adhesion functional solid lipid is: the adhesion functional solid lipid is generated by an amidation reaction between the carboxyl functional group of stearic acid and the amino functional group of dopamine.

[0013] Preferably, the plant essential oil component containing an isobutenyl group, an n-hexane skeleton or a hydroxyl group and having the function of refreshing the mind and relieving drowsiness includes one or a combination of menthol, borneol, lime oil, patchouli oil, lavender oil, sweet basil oil, geranium oil, clove oil and eucalyptus globulus leaf oil.

[0014] Preferably, the formula of the composite solid lipid is: 55-75wt% glyceryl monostearate, 10-20wt% isobutylene-containing solid lipid, 10-20wt% n-hexane skeleton-containing solid lipid and 3-8wt% adhesion-functional solid lipid.

[0015] Preferably, the composition comprises the following raw materials in parts by weight:

[0016] 2-3 parts menthol;

[0017] 1-1.5 parts borneol;

[0018] 1-1.5 parts lime oil;

[0019] 0.8-1.2 parts patchouli oil;

[0020] 0.8-1.2 parts lavender oil;

[0021] 0.8-1.2 parts sweet basil oil;

[0022] 0.5-1.0 parts geranium oil;

[0023] 0.3-0.8 parts of clove oil;

[0024] 0.3-0.8 parts of eucalyptus globulus leaf oil;

[0025] 20-40 parts of composite solid lipids;

[0026] 3-8 parts surfactant;

[0027] 80-120 parts water.

[0028] Preferably, the surfactant is one or a combination of two of Tween 80, Span 80, egg yolk lecithin, and Poloxamer 188.

[0029] Preferably, the preparation method of the composition for refreshing the mind and relieving drowsiness is: ingredients are prepared according to the formula of the composition and mixed evenly, and the composition for refreshing the mind and relieving drowsiness is prepared at 70-78° C. by a high-pressure homogenization method.

[0030] Preferably, the composition for refreshing the mind and relieving fatigue is used in aromatherapy products. Beneficial effects

[0031] The invention uses stearic acid as a basic raw material to synthesize an isobutylene-containing solid lipid, a n-hexane skeleton-containing solid lipid, and an adhesion-functional solid lipid. The isobutylene-containing solid lipid is preassembled with lime oil, lavender oil, sweet basil oil, geranium oil, and eucalyptus globulus leaf oil containing isobutylene groups based on the adsorption between similar structures. The n-hexane skeleton-containing solid lipid is preassembled with menthol, borneol, and patchouli oil containing n-hexane skeletons based on the adsorption between similar structures. The adhesion-functional solid lipid is preassembled with clove oil based on hydrogen bond adhesion. The lipid-essential oil mixture is then compounded with a conventional monostearate glyceryl solid lipid to obtain a lipid-essential oil mixture.

[0032] The lipid-essential oil mixture is emulsified with an aqueous phase by a high-pressure homogenization method to prepare a composition for refreshing the mind and relieving drowsiness, namely, a nanostructured lipid carrier encapsulating a functional component (plant essential oil) for refreshing the mind and relieving drowsiness;

[0033] Experimental results show that the composition for refreshing the mind and relieving drowsiness prepared by the present invention has a release rate as low as 81.5% in 12 hours, showing excellent sustained-release performance, which can effectively prolong the release time of the refreshing and relieving drowsiness functional components and improve the stability of the plant essential oil.

[0034] The composition for refreshing the mind and relieving fatigue prepared by the present invention can be used to prepare aromatherapy products. DETAILED DESCRIPTION

[0035] The present invention forms a composite solid lipid by mixing independently developed isobutylene-type solid lipids, n-hexane-skeleton-type solid lipids and adhesion-functional solid lipids with conventional monostearate. On the one hand, the isobutylene-type solid lipids and n-hexane-skeleton-type solid lipids have structures similar to those of essential oil components, thereby utilizing the principle of similar compatibility to enhance the compatibility of the solid lipids with the essential oil components. On the other hand, the stability of the nanostructured lipid carrier is further improved by utilizing the highly adhesive catechol structure in the adhesion-functional solid lipids. Example 1:

[0036] A composition I for refreshing the mind and relieving fatigue, comprising the following raw materials in parts by weight:

[0037] 2.5 parts menthol;

[0038] 1.3 parts borneol;

[0039] 1.3 parts lime oil;

[0040] 1.0 part patchouli oil;

[0041] 1.0 part lavender oil;

[0042] 1.0 part sweet basil oil;

[0043] 0.9 parts of geranium oil;

[0044] 0.5 parts clove oil;

[0045] 0.5 parts of eucalyptus globulus leaf oil;

[0046] 30 parts of composite solid lipids;

[0047] 5 parts Tween 80 surfactant;

[0048] 100 parts water;

[0049] The formula of the composite solid lipid is: 65wt% glyceryl monostearate, 15wt% isobutylene-containing solid lipid, 15wt% n-hexane skeleton-containing solid lipid and 5wt% adhesion functional solid lipid;

[0050] The preparation method of the isobutylene-containing solid lipid is as follows: the carboxyl functional group of stearic acid and the hydroxyl functional group of citronellol undergo an esterification reaction to generate an isobutylene-containing solid lipid, the chemical structure of which is:

[0051] ;

[0052] The specific experimental steps for preparing isobutylene-containing solid lipids are as follows: 2.8 g of stearic acid, 1.5 g of citronellol, and 50 mL of N,N-dimethylformamide were added to a three-necked flask and stirred at room temperature for 30 minutes to dissolve. Then, 10 mL of N,N-dimethylformamide solution containing 1.1 g of p-toluenesulfonic acid was added dropwise to the three-necked flask, the temperature was raised to 70°C, and the reaction was stirred for 12 hours. The mixture was cooled to room temperature, and the solvent was removed by rotary evaporation. The solid lipids were washed with a 5 wt% sodium bicarbonate aqueous solution and a saturated sodium chloride aqueous solution in sequence, and dried under vacuum to obtain isobutylene-containing solid lipids.

[0053] The nuclear magnetic resonance hydrogen spectrum of the solid lipid containing isobutylene group is characterized as follows: 1 H NMR (CDCl3, 400MHz) δ: 0.86-0.94(m, 6H), 1.21-1.31(m, 28H), 1.45-1.71(m, 13H), 1.98-2.03(m, 2H), 2.29-2.32(t, 2H), 4.14-4.17(t, 2H), 5.07-5.10(t, 1H);

[0054] The preparation method of the solid lipid containing a normal hexane skeleton is as follows: the carboxyl functional group of stearic acid and the hydroxyl functional group of natural menthol undergo an esterification reaction to generate a solid lipid containing a normal hexane skeleton, the chemical structure of which is:

[0055] ;

[0056] The specific experimental steps for preparing a solid lipid containing a n-hexane skeleton are as follows: 2.8 g of stearic acid, 1.5 g of natural menthol, and 50 mL of N,N-dimethylformamide are added to a three-necked flask, stirred and dissolved at room temperature for 30 minutes, then 10 mL of an N,N-dimethylformamide solution containing 1.3 g of p-toluenesulfonic acid is added dropwise to the three-necked flask, the temperature is raised to 70°C, and the reaction is stirred for 15 hours. The mixture is cooled to room temperature, and the solvent is removed by rotary evaporation. The solid lipid containing a n-hexane skeleton is washed with a 5 wt% sodium bicarbonate aqueous solution and a saturated sodium chloride aqueous solution in sequence, and vacuum dried to obtain a solid lipid containing a n-hexane skeleton.

[0057] The nuclear magnetic resonance hydrogen spectrum of the solid lipid containing n-hexane skeleton is characterized as follows: 1 H NMR (CDCl3, 400MHz) δ: 0.85-0.92(m, 12H), 1.23-1.31(m, 28H), 1.41-1.61(m, 7H), 1.75-1.82(m, 3H), 1.88-1.97(m, 1H), 2.29-2.33(t, 2H), 4.83-4.89(m, 1H);

[0058] The preparation method of the adhesion functional solid lipid is as follows: the carboxyl functional group of stearic acid and the amino functional group of dopamine [2-(3,4-dihydroxyphenyl)ethylamine] undergo amidation reaction to generate the adhesion functional solid lipid, the chemical structure of which is:

[0059] ;

[0060] The specific experimental steps for preparing the adhesion-functional solid lipid are as follows: under nitrogen protection, 2.8 g of stearic acid, 1.5 g of dopamine, and 50 mL of N,N-dimethylformamide were added to a three-necked flask and stirred at room temperature for 30 minutes to dissolve. Then, 10 mL of N,N-dimethylformamide solution containing 1.0 g of N,N'-dicyclohexylcarbodiimide was added to the three-necked flask, the temperature was raised to 70°C, and the mixture was stirred for 8 hours. The mixture was cooled to room temperature, the solvent was removed by rotary evaporation, and the mixture was washed with dichloromethane and dried in vacuo to obtain the adhesion-functional solid lipid.

[0061] The nuclear magnetic resonance hydrogen spectrum of the adhesion functional solid lipid is characterized as follows: 1 H NMR (CDCl3, 400MHz) δ: 0.87-0.91 (t, 3H), 1.25-1.35 (m, 28H), 1.54-1.61 (m, 2H), 2.13-2.17 (t, 2H), 2.62-2.65 (t, 2H), 3.38-3.42 (t, 2H), 6.43-6.72 (m, 3H). Example 2:

[0062] A method for preparing a composition I for refreshing the mind and relieving fatigue comprises the following steps:

[0063] Step 1: Evenly mix 2.5 g of menthol, 1.3 g of borneol, 1.3 g of lime oil, 1.0 g of patchouli oil, 1.0 g of lavender oil, 1.0 g of sweet basil oil, 0.9 g of geranium oil, 0.5 g of clove oil, and 0.5 g of eucalyptus globulus leaf oil to obtain a plant essential oil component;

[0064] Step 2: 19.5 g of glyceryl monostearate, 4.5 g of isobutylene-containing solid lipid, 4.5 g of n-hexane skeleton-containing solid lipid, and 1.5 g of adhesion-functional solid lipid were mixed evenly to form a composite solid lipid;

[0065] Step 3: A high-pressure homogenization method is used to prepare a composition I for refreshing the mind and relieving fatigue. The composite solid lipid prepared in step 2 and the plant essential oil component prepared in step 1 are added to a round-bottom flask, heated to 75°C and heated for 5 minutes to a molten state to form a lipid-essential oil mixture. Then, 5g of Tween 80 surfactant is dissolved in 100mL of water, heated to 75°C and stirred at a speed of 400 r / min for 5 minutes to form a surfactant-water phase mixture. Then, the surfactant-water phase mixture is added to the lipid-essential oil mixture at 75°C, and a high-pressure homogenizer is used to form a colostrum. The speed of the high-pressure homogenizer is 25000r / min, the pressure is 600bar, and the number of cycles is 4. The colostrum is also ultrasonically dispersed while hot and cooled to room temperature to obtain a composition I for refreshing the mind and relieving fatigue. Example 3:

[0066] A composition II for refreshing the mind and relieving drowsiness. The formula of the composition II differs from that of the composition I for refreshing the mind and relieving drowsiness only in that the formula of the composite solid lipid is: 55wt% glyceryl monostearate, 20wt% isobutylene-containing solid lipid, 20wt% n-hexane skeleton-containing solid lipid, and 5wt% adhesion-functional solid lipid. The preparation method of the composition II is the same as that of the composition I for refreshing the mind and relieving drowsiness. Example 4:

[0067] A composition III for refreshing the mind and relieving drowsiness. Its formula differs from that of composition I for refreshing the mind and relieving drowsiness only in that the formula of the composite solid lipid is: 75wt% glyceryl monostearate, 10wt% isobutylene-containing solid lipid, 10wt% n-hexane-based solid lipid, and 5wt% adhesion-functional solid lipid. The preparation method of the composite solid lipid is the same as that of composition I for refreshing the mind and relieving drowsiness. Embodiment 5:

[0068] The invention discloses an application of a composition for refreshing the mind and relieving drowsiness: 100 g of gypsum powder is mixed with 50 mL of water, 4 g of the composition for refreshing the mind and relieving drowsiness is added before pouring into a mold, the mixture is allowed to stand for 2 hours to harden, and the edges of the gypsum are gently pressed to release the mixture from the mold, thereby obtaining an aromatherapy product that can naturally emit a scent. Performance testing:

[0069] The encapsulation efficiency and release rate of the composition for refreshing the mind and relieving fatigue were tested. The specific steps are as follows:

[0070] Step 1: Scanning a plant essential oil component consisting of 2.5 g menthol, 1.3 g borneol, 1.3 g lime oil, 1.0 g patchouli oil, 1.0 g lavender oil, 1.0 g sweet basil oil, 0.9 g geranium oil, 0.5 g clove oil, and 0.5 g eucalyptus globulus leaf oil at full wavelength using a UV-visible spectrophotometer, recording the maximum absorption wavelength λmax, and dissolving the above plant essential oil components in anhydrous ethanol to prepare plant essential oil component solutions of different concentrations (including 7 groups with concentrations of 0.01, 0.03, 0.05, 0.08, 0.1, 0.13, and 0.15 g / mL), reading the absorbance values ​​of the plant essential oil component solutions of different concentrations at λmax using a UV-visible spectrophotometer, performing linear regression analysis with concentration as the abscissa and absorbance as the ordinate, and drawing a standard curve equation;

[0071] Step 2: The composition for refreshing the mind and relieving fatigue containing the Mg plant essential oil component is placed in a dialysis bag and immersed in a beaker filled with VmL of water. The mixture is stirred at low speed for Tmin at room temperature. The absorbance of the aqueous solution in the beaker is measured by an ultraviolet spectrophotometer. The absorbance value is substituted into the standard curve equation to obtain the concentration C of the free plant essential oil component. The encapsulation efficiency and release rate are calculated as follows:

[0072] Encapsulation efficiency (%) = [1-(C × V) / M] × 100%;

[0073] When T=0 min, the concentration C0 of the free plant essential oil component is measured. At this time, the initial encapsulation efficiency of the composition for refreshing the mind and relieving fatigue is measured. The initial encapsulation efficiency (%) = [1-(C0×V) / M]×100%;

[0074] When T=6h, the concentration C1 of the free plant essential oil component is measured. At this time, the 6-hour encapsulation efficiency of the composition for refreshing the mind and relieving fatigue is measured. The 6-hour encapsulation efficiency (%) = [1-(C1×V) / M]×100%;

[0075] 6h release rate (%) = [1-6h encapsulation rate / initial encapsulation rate] × 100%;

[0076] The 12h release rate was tested using the same method as above;

[0077] The above test results are shown in Table 1-2 below;

[0078] Table 1 Performance test results of the composition for refreshing the mind and relieving fatigue

[0079]

[0080] Note: The only difference between the comparative example in Table 1 and Composition I for refreshing the mind and relieving drowsiness is that 100 wt% of monostearate glyceryl is used to replace the composite solid lipid (65 wt% of monostearate glyceryl, 15 wt% of isobutylene-containing solid lipid, 15 wt% of n-hexane skeleton-containing solid lipid, and 5 wt% of adhesion-functional solid lipid);

[0081] Table 2 Performance test results of the composition for refreshing the mind and relieving fatigue

[0082]

[0083] By comprehensively analyzing the above experimental results, the following conclusions can be drawn:

[0084] (1) Compared with the solid lipid formed by conventional monostearate alone, the composite solid lipid independently developed by the present invention has achieved a beneficial technical effect of significantly improving the encapsulation efficiency of plant essential oil components;

[0085] (2) The composition for refreshing the mind and relieving fatigue prepared by the present invention has excellent sustained-release performance, can prolong the release time of the plant essential oil components, and improve the stability of the plant essential oil components.

Claims

1. A composition for refreshing the mind and relieving fatigue, characterized in that: The composition is pre-assembled by composite solid lipids and plant essential oil components containing isobutylene groups, n-hexane skeletons or hydroxyl groups and having the functions of refreshing the mind and relieving fatigue based on the adsorption or hydrogen bond adhesion between similar structures, and then emulsified with an aqueous phase by a high-pressure homogenization method; The composite solid lipid is composed of an isobutylene-containing solid lipid, a hexane-containing solid lipid, an adhesion-functional solid lipid, and glyceryl monostearate. The chemical structural formula of the isobutylene-containing solid lipid is: ； The chemical structural formula of the solid lipid containing n-hexane skeleton is: ； The chemical structural formula of the adhesion functional solid lipid is: ； The plant essential oil component containing an isobutylene group, an n-hexane skeleton or a hydroxyl group and having the function of refreshing the mind and relieving drowsiness includes one or a combination of menthol, borneol, lime oil, patchouli oil, lavender oil, sweet basil oil, geranium oil, clove oil and eucalyptus globulus leaf oil; The formula of the composite solid lipid is: 55-75wt% of glyceryl monostearate, 10-20wt% of isobutylene-containing solid lipid, 10-20wt% of n-hexane skeleton-containing solid lipid and 3-8wt% of adhesion functional solid lipid.

2. A composition for refreshing the mind and relieving fatigue according to claim 1, characterized in that: The preparation method of the isobutylene-containing solid lipid comprises the following steps: an esterification reaction is carried out between the carboxyl functional group of stearic acid and the hydroxyl functional group of citronellol to generate the isobutylene-containing solid lipid.

3. A composition for refreshing the mind and relieving fatigue according to claim 1, characterized in that: The preparation method of the solid lipid containing a normal hexane skeleton comprises the following steps: an esterification reaction is carried out between the carboxyl functional group of stearic acid and the hydroxyl functional group of natural menthol to generate the solid lipid containing a normal hexane skeleton.

4. A composition for refreshing the mind and relieving fatigue according to claim 1, characterized in that: The preparation method of the adhesion functional solid lipid comprises the following steps: the carboxyl functional group of stearic acid and the amino functional group of dopamine undergo an amidation reaction to generate the adhesion functional solid lipid.

5. A composition for refreshing the mind and relieving fatigue according to any one of claims 1 to 4, characterized in that: The composition comprises the following raw materials in parts by weight: 2-3 parts menthol; 1-1.5 parts borneol; 1-1.5 parts lime oil; 0.8-1.2 parts patchouli oil; 0.8-1.2 parts lavender oil; 0.8-1.2 parts sweet basil oil; 0.5-1.0 parts geranium oil; 0.3-0.8 parts of clove oil; 0.3-0.8 parts of eucalyptus globulus leaf oil; 20-40 parts of composite solid lipids; 3-8 parts surfactant; 80-120 parts water.

6. A composition for refreshing the mind and relieving fatigue according to claim 5, characterized in that: The surfactant is one or a combination of two of Tween 80, Span 80, egg yolk lecithin, and Poloxamer 188.

7. A composition for refreshing the mind and relieving fatigue according to claim 5, characterized in that: The preparation method of the composition for refreshing the mind and relieving drowsiness comprises the following steps: preparing ingredients according to the formula of the composition and mixing them evenly, and preparing the composition for refreshing the mind and relieving drowsiness by a high-pressure homogenization method at 70-78° C.

8. A composition for refreshing the mind and relieving fatigue according to any one of claims 1 to 4, characterized in that: Application of the composition in preparing aromatherapy products.

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