Multipurpose lily dwarfing method and application thereof

Abstract

The invention discloses a multipurpose lily dwarfing method and application thereof. The method comprises the following steps: (1) disinfection of bulbs: selecting lily bulbs for disinfection; (2) low-temperature germination accelerating: embedding the disinfected seed balls by using a completely soaked germination accelerating substrate, and putting the seed balls into a refrigeration house for germination accelerating; (3) plant field planting: performing potted planting on the seed balls subjected to germination acceleration by using a planting substrate; 4, pesticide spraying, wherein 80 mg / L paclobutrazol solution is adopted to spray plants which grow to 10 cm and have unfolded leaves once. According to the lily dwarfing method, growth of stems of lilies is accurately inhibited, meanwhile, the number of times of pesticide spraying is reduced, dwarfing of lily plants is achieved, and the ornamental effect of potted lilies is improved.

Description

Technical Field

[0001] The present invention relates to the field of horticulture technology, specifically to a method for dwarfing multi-purpose lilies and its application. Specifically, it relates to a dwarfing method for two new lily varieties, 'Fei Xiang Shao Hua' and 'Fei Xiang Tian Cheng', independently developed by the lily research team of Nanjing Agricultural University. These lilies have excellent comprehensive traits, are popular in the market, and have multiple uses, such as ornamental, edible, and tea. Background Art

[0002] Lilies (Lilium spp.) belong to the genus Lilium in the family Liliaceae. Their large, elegant flowers, rich and varied colors, graceful and graceful appearance, and lingering fragrance make them a world-renowned and traditional Chinese flower, known as the "King of Bulbs." They are also deeply loved for their symbolic promise of a long and happy marriage. Lilies can be used not only as cut flowers and potted plants but also in landscaping. Some varieties are edible and medicinal, possessing both high ornamental and commercial value.

[0003] With the development of the floriculture industry, we've seen a growing demand for potted lilies. Unlike the qualities required for cut flowers, potted lilies require plants of moderate height, dark green leaves, a compact shape, and a long flowering period. However, commonly sold Asian lily varieties exceed 1 meter in height, making them unsuitable for potted cultivation. Excessive stem height can easily lead to tilting or even breaking. Furthermore, current methods for dwarfing lilies often involve spraying with pesticides, with varying concentrations and frequency. Excessive application can lead to plant damage and environmental pollution. Therefore, a method for dwarfing lilies while reducing pesticide use is needed. Summary of the Invention

[0004] The present invention aims to provide a multi-purpose lily dwarfing method and its application. This method inhibits stem growth and controls internode length while reducing the need for pesticide spraying, achieving dwarfing of lily plants and improving the ornamental quality of potted lilies. This invention establishes a highly efficient dwarfing method for the new lily varieties 'Feixiang Shaohua' and 'Feixiang Tiancheng,' providing scientific and technological support for their large-scale industrialization.

[0005] The purpose of the present invention can be achieved through the following technical solutions:

[0006] In a first aspect, the present invention claims a method for dwarfing multi-purpose lilies, the method comprising the following steps:

[0007] (1) Bulb disinfection: Select bulbs of lily varieties 'Fei Xiang Shao Hua' and / or 'Fei Xiang Tian Cheng' for disinfection;

[0008] (2) Low temperature germination: The sterilized bulbs are embedded in a completely soaked germination matrix and placed in a cold storage for germination;

[0009] (3) Planting: Plant the germinated bulbs in pots using a planting medium;

[0010] (4) Spraying: When the plants have grown to 10 cm and their leaves have unfolded, spray them once with 80 mg / L paclobutrazol solution.

[0011] Furthermore, the lily bulbs are disinfected by soaking them in 700 times carbendazim for half an hour for disinfection.

[0012] Furthermore, the germination matrix and the planting matrix are both matrices with a volume ratio of peat: vermiculite: pine needles = 5:3:2.

[0013] Furthermore, the germinated bulbs are bulbs that have been germinated to 8 cm and have two circles of stem roots growing on the bulbs.

[0014] Furthermore, the germination temperature is 12-14°C.

[0015] Furthermore, the humidity of the germination substrate is 30-50%, preferably 40%.

[0016] In a second aspect, the present invention seeks to protect the application of the above method in dwarfing the lily varieties 'Feixiang Tiancheng' and / or 'Feixiang Shaohua'.

[0017] In a specific embodiment of the present invention, the lily variety is 'Fei Xiang Shao Hua' or 'Fei Xiang Tian Cheng', and the pesticide used is paclobutrazol. The spray concentration is 80 mg / L, and the spray frequency is one. The spraying time is when the plant grows to 10 cm and the leaves are unfolded. The germination medium humidity is 40%, the bud length is 8 cm, and the germination temperature is 12-14°C.

[0018] The lily materials described in the lily dwarfing method of the present invention are new varieties independently developed and cultivated by the scientific research team of Nanjing Agricultural University in cooperation with domestic peers, namely, the functional lilies 'Feixiang Shaohua' and 'Feixiang Tiancheng'.

[0019] During the research process, the technicians of the present invention found that when the concentration and timing of spraying the agent are appropriate during the growth of lily, the target height can be reached by spraying once. Therefore, screening the type, concentration and spraying time of the spraying agent for lily is the main invention point of the present invention.

[0020] Beneficial effects of the present invention:

[0021] This invention establishes an optimal dwarfing technology system for lilies: the optimal spraying concentration of paclobutrazol for 'Fei Xiang Shao Hua' is 80 mg / L, the optimal number of sprayings is one, and the optimal spraying time is when the plant grows to 10 cm and the leaves are unfolded. Without affecting the normal growth of 'Fei Xiang Shao Hua' plants, the plant is dwarfed by 30%. The optimal spraying concentration of paclobutrazol for 'Fei Xiang Tian Cheng' is 80 mg / L, the optimal number of sprayings is one, and the optimal spraying time is when the plant grows to 10 cm and the leaves are unfolded. Without affecting the normal growth of 'Fei Xiang Tian Cheng' plants, the plant is dwarfed by 40%. This invention establishes a dwarfing technology for multi-purpose lilies that is highly specific, simple to operate, and low-cost, laying a good foundation for the industrial development of these two new lily varieties. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is the growth stage of the 'Fei Xiang Shao Hua' treatment group.

[0023] Figure 2 This is the growth stage of the 'Fei Xiang Shao Hua' blank group.

[0024] Figure 3 It is the dwarf effect state of 'Flying Time'.

[0025] Figure 4 This is the flowering state of 'Flying Shaohua'.

[0026] Figure 5 This is the growth stage of the 'Feixiang Tiancheng' treatment group.

[0027] Figure 6 This is the growth stage of the 'Feixiang Tiancheng' blank group.

[0028] Figure 7 This is the dwarfing effect of 'Flying Sky Orange'.

[0029] Figure 8 This is the flowering situation of 'Flying Sky Orange'. DETAILED DESCRIPTION

[0030] Below in conjunction with the figures and examples, the specific embodiments of the present invention are further described in detail. The following examples are only used to illustrate the present invention and are not intended to limit the scope of protection of the present invention. Unless otherwise specified, the reagents and equipment used in the following examples are all commercially available or commonly used in the art, and the methods used in the following examples are all commonly used methods in the art.

[0031] Example 1

[0032] (1) Disinfection of bulbs

[0033] Select bulbs of the 'Fei Xiang Shao Hua' lily with a circumference of 14-16 cm, no external damage, and tightly packed and plump. Completely soak the bulbs in a solution containing 50% carbendazim powder in a 700-fold carbendazim solution of water. Remove after half an hour and place in a cool, dry place to sterilize the bulbs.

[0034] (2) Low temperature germination

[0035] Place a 3 cm thick germination substrate in a plastic planting basket that is 52 cm long, 35 cm wide, and 17 cm high. The volume ratio of the substrate is peat: vermiculite: pine needles = 5:3:2. Place the sterilized bulbs upward on the substrate, and then spread the same substrate on top of the bulbs with a thickness of 3 cm. Water it to a moisture content of 40% and place it in a cold storage at 12-14 ℃ until it sprouts 8 cm and grows two circles of stem roots.

[0036] (3) Planting

[0037] Remove the germinated bulbs from the substrate and place a 5 cm thick substrate in a plastic flowerpot with a size of 23 cm above, 20 cm high, and 16 cm below. The volume ratio of the substrate is peat: vermiculite: pine needles = 5:3:2. Place three germinated bulbs in an equilateral triangle on the substrate, and lean against the edge of the flowerpot. Spread the same substrate above the bulbs until it is 2 cm away from the mouth of the flowerpot. Water it thoroughly with water. Place the transplanted plants under a shade net for one week.

[0038] (4) Spraying of pesticides

[0039] For plants that have grown to 10 cm and have expanded leaves, the pesticide is applied by foliar spraying. When spraying on the leaves, use a pressure sprayer and spray until water droplets gather on the leaf surface without dripping. Select the appropriate pesticide type, concentration, and spraying frequency (see Table 1, Table 2, and Table 3).

[0040] Table 1 Types and concentrations of drugs

[0041]

[0042] Table 2 Plant spraying height

[0043]

[0044] Table 3 Number of times the pesticide was sprayed

[0045]

[0046] (6) Data processing

[0047] The repeated data were imported into SPSS for homogeneity test to obtain the standard deviation of each group of data and the difference between groups. The degree of difference was expressed in lowercase letters.

[0048] Result Analysis

[0049] (1) Selection of drug type and concentration

[0050] Table 4 Effect of spraying different pesticide types and concentrations on dwarfing of 'Fei Xiang Shao Hua'

[0051]

[0052] As shown in Table 4, different concentrations of paclobutrazol and chlormequat can dwarf the plants of 'Fei Xiang Shao Hua'. The dwarfing effect of paclobutrazol at a concentration of 80 mg / L was the best, and the plant height could reach 65.22 cm. Since the dwarfing effect of paclobutrazol reached its peak when sprayed at 80 mg / L, and began to decline at 100 mg / L, while chlormequat had the best effect at 2.5 g / L, and the dwarfing effect increased with the increase of chlormequat concentration, from the perspective of reducing the harm of the pesticide to the plant and the impact on the environment by applying a small amount of pesticide, spraying paclobutrazol can better reduce its impact and obtain a better dwarfing effect. Therefore, the subsequent test on the effect of the number of spraying on the dwarfing effect of 'Fei Xiang Shao Hua' was carried out with 80 mg / L paclobutrazol. Figure 1 and Figure 2 As shown, the dwarfing effect of the treated group during the growth stage was significant compared with the blank group.

[0053] (2) Selection of spraying height

[0054] Table 5 Effect of spraying at different heights on the dwarfing of 'Fei Xiang Shao Hua'

[0055]

[0056] As shown in Table 5, different spraying heights can dwarf the plants of 'Feixiang Shaohua', and reducing the spraying height can make the dwarfing effect of 'Feixiang Shaohua' more obvious. Therefore, a plant height of 10 cm can be selected as the optimal spraying height for dwarfing 'Feixiang Shaohua'.

[0057] (3) Selection of spraying frequency

[0058] Table 6 Effect of different spraying times on the dwarfing of 'Fei Xiang Shao Hua'

[0059]

[0060] As shown in Table 6, different spraying times can dwarf the plants of 'Fei Xiang Shao Hua', and the increase in the number of spraying times can make the dwarfing effect of 'Fei Xiang Shao Hua' more obvious. However, the effect of spraying paclobutrazol multiple times on the dwarfing effect of 'Fei Xiang Shao Hua' is not significant compared with spraying paclobutrazol once. Therefore, from the perspective of reducing the harm of the pesticide to the plant and the impact on the environment by applying a small amount of pesticide, spraying once can be selected as the optimal spraying frequency for dwarfing 'Fei Xiang Shao Hua' (e.g. Figure 3 shown).

[0061] (4) Effect of spraying times on flower morphology of 'Fei Xiang Shao Hua'

[0062] Table 7 Effect of spraying times on flower morphology of 'Fei Xiang Shao Hua'

[0063]

[0064] As shown in Table 7, the number of flowers, flower diameter, bud length, and pedicel thickness of 'Fei Xiang Shao Hua' were not significantly affected by different paclobutrazol spraying times compared with no spraying. Flower diameter, bud length, pedicel thickness, and petal thickness are all key indicators for measuring the ornamental characteristics of lilies, and the petal thickness was significantly thicker than that of no spraying. The increase in petal thickness helps to improve the ornamental effect of lilies. Therefore, it can be concluded that paclobutrazol spraying has a certain degree of improvement on the ornamental characteristics of 'Fei Xiang Shao Hua' (such as Figure 4 shown).

[0065] (5) Effect of spraying times on stem diameter of 'Fei Xiang Shao Hua'

[0066] Table 8 Effects of different spraying times on stem diameter of 'Fei Xiang Shao Hua'

[0067]

[0068] Table 8 shows that spraying paclobutrazol can increase the stem diameter of 'Fei Xiang Shao Hua'. The increase in stem diameter becomes more obvious with increasing spraying frequency, but the effect is not significant. Lily stem diameter is closely related to stem strength, so spraying paclobutrazol can increase stem strength.

[0069] (6) Effect of spraying times on photosynthetic rate of 'Fei Xiang Shao Hua'

[0070] Table 9 Effects of different spraying times on the photosynthetic rate of 'Fei Xiang Shao Hua'

[0071]

[0072] Table 9 shows that spraying paclobutrazol significantly increased the photosynthetic rate of 'Fei Xiang Shao Hua', while different spraying times had no significant effect on the photosynthetic rate. The photosynthetic rate of lilies is related to the accumulation of lily substances, so spraying paclobutrazol can increase the accumulation of lily substances.

[0073] (7) Effect of spraying times on the flowering time of 'Fei Xiang Shao Hua'

[0074] Table 10 Effect of spraying times on flowering time of 'Fei Xiang Shao Hua'

[0075]

[0076] As shown in Table 10, spraying paclobutrazol can delay the budding and flowering time of 'Fei Xiang Shao Hua', but can increase the average flowering period of 'Fei Xiang Shao Hua'.

[0077] (8) Effect of spraying times on leaf morphology of 'Fei Xiang Shao Hua'

[0078] Table 11 Effects of spraying times on leaf morphology of 'Fei Xiang Shao Hua'

[0079]

[0080] As shown in Table 11, the leaf length of 'Fei Xiang Shao Hua' was reduced to a certain extent when different paclobutrazol spraying times were used compared with no spraying, while the leaf width and thickness were increased to a certain extent, which could increase the area of ​​lily exposed to light, improve the photosynthetic rate, and also increase the stress resistance of lily.

[0081] Example 2

[0082] (1) Disinfection of bulbs

[0083] Select bulbs of the 'Fei Xiang Tian Cheng' lily with a circumference of 14-16 cm, no external damage, and tightly packed and plump. Completely soak the bulbs in a solution containing 50% carbendazim powder in a 700-fold carbendazim solution containing water. After half an hour, remove the bulbs and place them in a cool, dry place to sterilize them.

[0084] (2) Low temperature germination

[0085] Place a 3 cm thick germination substrate in a plastic planting basket that is 52 cm long, 35 cm wide, and 17 cm high. The volume ratio of the substrate is peat: vermiculite: pine needles = 5:3:2. Place the sterilized bulbs upward on the substrate, and then spread the same substrate on top of the bulbs with a thickness of 3 cm. Water it to a moisture content of 40% and place it in a cold storage at 12-14 ℃ until it sprouts 8 cm and grows two circles of stem roots.

[0086] (3) Planting

[0087] Remove the germinated bulbs from the substrate and place a 5 cm thick substrate in a plastic flowerpot with a size of 23 cm above, 20 cm high, and 16 cm below. The volume ratio of the substrate is peat: vermiculite: pine needles = 5:3:2. Place three germinated bulbs in an equilateral triangle on the substrate, and lean against the edge of the flowerpot. Spread the same substrate above the bulbs until it is 2 cm away from the mouth of the flowerpot. Water it thoroughly with water. Place the transplanted plants under a shade net for one week.

[0088] (4) Spraying of pesticides

[0089] For plants that have grown to 10 cm and have expanded leaves, the pesticide should be applied by foliar spraying. When spraying on the leaves, use a pressure sprayer and spray until water droplets gather on the leaf surface without dripping. Select the appropriate pesticide type, concentration, and spraying frequency (see Table 12, Table 13, Table 14).

[0090] Table 12 Types and concentrations of drugs

[0091]

[0092] Table 13 Plant spraying height

[0093]

[0094] Table 14 Number of times the pesticide was sprayed

[0095]

[0096] (6) Data processing

[0097] The repeated data were imported into SPSS for homogeneity test to obtain the standard deviation of each group of data and the difference between groups. The degree of difference was expressed in lowercase letters.

[0098] Result Analysis

[0099] (1) Selection of drug type and concentration

[0100] Table 15 Effect of spraying different pesticide types and concentrations on the dwarfing of 'Fei Xiang Tian Cheng'

[0101]

[0102] As shown in Table 15, different concentrations of paclobutrazol and chlormequat can dwarf the plants of 'Fei Xiang Tian Cheng'. The dwarfing effect of paclobutrazol at a concentration of 80 mg / L was the best, and the plant height could reach 55.45 cm. Since the dwarfing effect of paclobutrazol reached its peak when sprayed at 80 mg / L, and began to decline at 100 mg / L, while chlormequat had the best effect at 2.5 g / L, and the dwarfing effect increased with the increase of chlormequat concentration, from the perspective of reducing the harm of the pesticide to the plant and the environmental impact by applying a small amount of pesticide, spraying paclobutrazol can better reduce its impact and obtain a better dwarfing effect. Therefore, the subsequent test on the effect of the number of spraying on the dwarfing effect of 'Fei Xiang Tian Cheng' was conducted with 80 mg / L paclobutrazol. Figure 5 and Figure 6 As shown, the dwarfing effect of the treated group during the growth stage was significant compared with the blank group.

[0103] (2) Selection of spraying height

[0104] Table 16 Effect of spraying at different heights on the dwarfing of 'Fei Xiang Tian Cheng'

[0105]

[0106] As shown in Table 16, different spraying heights can dwarf the plants of 'Feixiang Tiancheng', and reducing the spraying height can make the dwarfing effect of 'Feixiang Tiancheng' more obvious. Therefore, a plant height of 10 cm can be selected as the optimal spraying height for dwarfing 'Feixiang Tiancheng'.

[0107] (3) Selection of spraying frequency

[0108] Table 17 Effect of different spraying times on the dwarfing of 'Fei Xiang Tian Cheng'

[0109]

[0110] As shown in Table 17, different spraying times can dwarf the plants of 'Fei Xiang Tian Cheng', and the increase in spraying times can make the dwarfing effect of 'Fei Xiang Tian Cheng' more obvious. However, the effect of spraying paclobutrazol multiple times on the dwarfing effect of 'Fei Xiang Tian Cheng' is not significant compared with spraying paclobutrazol once. Therefore, from the perspective of reducing the harm of pesticides to plants and the impact on the environment by applying a small amount of pesticide, spraying once can be selected as the optimal spraying frequency for dwarfing 'Fei Xiang Tian Cheng' (e.g. Figure 7 shown).

[0111] (4) Effect of spraying times on flower morphology of 'Fei Xiang Tian Cheng'

[0112] Table 18 Effect of spraying times on flower morphology of 'Fei Xiang Tian Cheng'

[0113]

[0114] As shown in Table 18, the number of flowers, flower diameter, bud length, and pedicel thickness of 'Fei Xiang Tian Cheng' were not significantly affected by different paclobutrazol spraying times compared with no spraying. Flower diameter, bud length, pedicel thickness, and petal thickness are all key indicators for measuring the ornamental characteristics of lilies, and the petal thickness was significantly thicker than that of no spraying. The increase in petal thickness helps to improve the ornamental effect of lilies. Therefore, it can be concluded that paclobutrazol spraying has a certain degree of improvement on the ornamental characteristics of 'Fei Xiang Tian Cheng' (such as Figure 8 shown).

[0115] (5) Effect of spraying times on stem diameter of 'Fei Xiang Tian Cheng'

[0116] Table 19 Effects of different spraying times on stem diameter of 'Fei Xiang Tian Cheng'

[0117]

[0118] Table 19 shows that spraying paclobutrazol can increase the stem diameter of 'Fei Xiang Tian Cheng'. The increase in stem diameter becomes more obvious with increasing spraying frequency, but the effect is not significant. Lily stem diameter is closely related to stem strength, so spraying paclobutrazol can increase lily stem strength.

[0119] (6) Effect of spraying times on photosynthetic rate of 'Fei Xiang Tian Cheng'

[0120] Table 20 Effects of different spraying times on the photosynthetic rate of 'Fei Xiang Tian Cheng'

[0121]

[0122] Table 20 shows that spraying paclobutrazol significantly increased the photosynthetic rate of 'Fei Xiang Tian Cheng', while different spraying times had no significant effect on the photosynthetic rate. The photosynthetic rate of lilies is related to the amount of material accumulated in the lily, so spraying paclobutrazol can increase the amount of material accumulated in the lily.

[0123] (7) Effect of spraying times on the flowering time of 'Fei Xiang Tian Cheng'

[0124] Table 21 Effect of spraying times on flowering time of 'Fei Xiang Tian Cheng'

[0125]

[0126] As shown in Table 21, spraying paclobutrazol can delay the budding and flowering time of 'Feixiang Tiancheng', but can increase the average flowering period of 'Feixiang Tiancheng'.

[0127] (8) Effect of spraying times on leaf morphology of 'Fei Xiang Tian Cheng'

[0128] Table 22 Effect of spraying times on leaf morphology of 'Fei Xiang Tian Cheng'

[0129]

[0130] As shown in Table 22, the leaf length of 'Fei Xiang Tian Cheng' was reduced to a certain extent when different paclobutrazol spraying times were used compared with no spraying, while the leaf width and thickness were increased to a certain extent, which could increase the area of ​​lily exposed to light, improve the photosynthetic rate, and also increase the stress resistance of lily.

[0131] In summary, the results of the above examples indicate that the optimal spraying agent for 'Fei Xiang Shao Hua' is paclobutrazol, with an optimal spraying concentration of 80 mg / L and an optimal spraying frequency of one. This results in a dwarfing effect of 30%, while also increasing the stem diameter, photosynthetic rate, and average flowering period of 'Fei Xiang Shao Hua', without significantly affecting flower morphology. The optimal spraying agent for 'Fei Xiang Tian Cheng' is paclobutrazol, with an optimal spraying concentration of 80 mg / L and an optimal spraying frequency of one. This results in a dwarfing effect of 40%, while also increasing the stem diameter, photosynthetic rate, and average flowering period of 'Fei Xiang Tian Cheng', without significantly affecting flower morphology. This present invention establishes two optimal dwarfing technology systems for multi-purpose lily varieties.

Claims

1. A multi-purpose lily dwarfing method, characterized in that: The method comprises the following steps: (1) Bulb disinfection: Select bulbs of lily varieties 'Fei Xiang Shao Hua' and / or 'Fei Xiang Tian Cheng' for disinfection; (2) Low temperature germination: The sterilized bulbs are embedded in a completely soaked germination matrix and placed in a cold storage for germination; (3) Planting: Plant the germinated bulbs in pots using a planting medium; (4) Spraying: When the plants have grown to 10 cm and their leaves have unfolded, spray them once with 80 mg / L paclobutrazol solution.

2. The method according to claim 1, characterized in that The disinfection method of the lily bulbs is as follows: using 700 times carbendazim to soak and disinfect for half an hour.

3. The method according to claim 1, characterized in that The germination matrix and the planting matrix are both matrices with a volume ratio of peat: vermiculite: pine needles = 5:3:

2.

4. The method according to claim 1, wherein The germinated bulbs are bulbs that have been germinated to 6-10 cm and have two circles of stem roots growing on the bulbs.

5. The method according to claim 1, characterized in that The germination temperature is 12-14°C.

6. The method according to claim 1, characterized in that The humidity of the germination substrate is 30%-50%, preferably 40%.

7. Use of the method according to any one of claims 1 to 6 in dwarfing the lily varieties 'Feixiang Tiancheng' and / or 'Feixiang Shaohua'.

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