Incubation device and biochemical reaction system

Abstract

The incubation device comprises a base, a heat dissipation module and a heating module, the heat dissipation module and the heating module are arranged on the base, the heating module comprises a temperature control structure and a heating seat, the temperature control structure is arranged on the heat dissipation module, the heating seat is arranged on the temperature control structure, at least one heating groove is formed in the heating seat, and the heating groove is used for containing a container containing a sample. The temperature control structure is used for regulating and controlling the temperature of the heating tank, so that a sample in the container reaches a preset temperature, and the inner wall of the heating tank is provided with a profiling structure corresponding to the outer wall of the container. According to the incubation device, the inner wall of the heating groove is subjected to profiling design, so that the inner wall of the heating groove can be more attached to the outer wall of the container, the contact area of the heating groove and the container is increased, the heat conduction efficiency is improved, heating is more uniform, and incubation of a sample is more facilitated.

Description

Technical Field

[0001] The present application relates to the technical field of biochemical substance analysis, and in particular to an incubation device and a biochemical reaction system. Background Art

[0002] With the development of sequencing technology, third-generation sequencing technology, which does not require a complex library construction process, has emerged and has received widespread attention in the academic community in recent years. High-yield library preparation is crucial for third-generation sequencing technology. The purpose of library preparation is to convert the biological sample to be analyzed (such as DNA or RNA sequences) into a library sample that can be recognized by a high-throughput sequencer, thereby facilitating subsequent analysis and interpretation. During the sequencing library construction process, the biological sample and the required reagents need to be incubated to create suitable reaction conditions for the biological sample.

[0003] However, current incubation devices consume high power and have low thermal conductivity, which directly affects the incubation results and ultimately the amount of library access. Furthermore, the incubation devices are large in size, hindering the miniaturization of biochemical reaction systems and reducing the space utilization of the biochemical reaction systems. Summary of the Invention

[0004] In order to solve at least one of the above defects, it is necessary to propose an incubation device in the present application.

[0005] In addition, an embodiment of the present application also provides an incubation system using the incubation device.

[0006] In a first aspect, an embodiment of the present application provides an incubation device, comprising: a base, a heat dissipation module and a heating module arranged on the base, the heating module comprising a temperature control structure arranged on the heat dissipation module and a heating seat arranged on the temperature control structure, the heating seat having at least one heating groove, the heating groove being used to accommodate a container loaded with a sample, the temperature control structure being used to regulate the temperature of the heating groove so that the sample in the container reaches a preset temperature, wherein the inner wall of the heating groove has a contoured structure corresponding to the outer wall of the container.

[0007] In some possible embodiments, along the depth direction of the heating groove, the heating groove includes a first groove and a second groove that are interconnected, the inner diameter of the first groove is larger than the inner diameter of the second groove, and the inner diameter of the second groove decreases successively in the direction away from the first groove.

[0008] In some possible embodiments, along the depth direction of the heating groove, the heating groove includes a first groove, a second groove and a third groove that are connected in sequence, the inner diameter of the first groove is larger than the inner diameters of the second groove and the third groove, the inner diameter of the second groove decreases in the direction away from the first groove, and the inner diameter of the third groove is equal to the inner diameter of the end of the second groove away from the first groove.

[0009] In some possible embodiments, the heating seat includes a heating base and a plurality of heating troughs located on a side of the heating base away from the temperature control structure, each of the heating troughs has a heating trough, and there is a gap between two adjacent heating troughs so that the plurality of heating troughs are independently arranged.

[0010] In some possible embodiments, the heating base and the plurality of heating slots are an integrated structure, and the heating slots extend to the heating base.

[0011] In some possible embodiments, the distance between the bottom of the heating tank and the surface of the heating base close to the temperature control structure is 2 mm to 4 mm.

[0012] In some possible embodiments, steps are respectively provided on opposite sides of the heating base, a first connecting hole is provided through the steps, an insulating block is provided on the step, a boss is provided on the side of the insulating block close to the first connecting hole, the boss extends into the first connecting hole, a second connecting hole is provided through the boss, the second connecting hole is provided through the insulating block, and the connecting member is connected to the heat dissipation module by passing through the second connecting hole and the first connecting hole.

[0013] In some possible embodiments, the incubation device further includes a heat insulation cover wrapped around the outside of the heating module, the heat insulation cover is provided with a mounting hole corresponding to the heating groove, the side wall of the mounting hole at one end away from the heat dissipation module protrudes toward the inside of the mounting hole to form a protrusion, and the protrusion is provided corresponding to the end face of the heating groove away from the heat dissipation module to cover the end face of the heating groove.

[0014] In some possible embodiments, the heat dissipation module includes a heat dissipation bracket provided on the base, and a heat dissipation fan and a radiator provided on the heat dissipation bracket, the temperature control structure is located on the radiator, the heat dissipation fan is located on the side of the radiator away from the heating module, the air inlet of the heat dissipation fan faces the base, and the air outlet of the heat dissipation fan faces the radiator.

[0015] In a second aspect, an embodiment of the present application further provides a biochemical reaction system, which includes: a biochemical reaction platform and the incubation device as described above.

[0016] The incubation device provided in the embodiment of the present application, by contouring the inner wall of the heating tank, can make the inner wall of the heating tank fit more closely with the outer wall of the container, increase the contact area between the heating tank and the container, and thus improve the heat conduction efficiency. At the same time, this contouring design also makes the heating of the sample in the container more uniform, which is more conducive to the incubation of the sample; the heating seat adopts a hollow design, which is about 1 / 2 smaller in volume than the non-hollow design, resulting in a small heating area, a fast heat conduction speed, and an effectively shortened heating and cooling time, which is conducive to reducing energy consumption. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following is a brief introduction to the drawings required for use in the embodiments of the present application. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0018] Figure 1 Schematic diagram of the structure of an incubation device according to one embodiment of the present application.

[0019] Figure 2 for Figure 1 Schematic diagram of the structure of the supporting container on the incubation device.

[0020] Figure 3 for Figure 1 Exploded view of the incubation device.

[0021] Figure 4 for Figure 3 Schematic diagram of the structure of the middle base.

[0022] Figure 5 for Figure 3 Exploded view of the heat dissipation module.

[0023] Figure 6 This is a structural diagram of a heat-conducting layer provided between the temperature control structure, the radiator and the heating seat in one embodiment of the present application.

[0024] Figure 7 This is a partial cross-sectional view of a container placed in a heating tank in one embodiment of the present application.

[0025] Figure 8 for Figure 1 Partial cross-section along line VIII-VIII.

[0026] Figure 9 for Figure 8 Schematic diagram of the structure of the middle insulation block.

[0027] Figure 10 Schematic diagram of the structure of a biochemical reaction system in one embodiment of the present application.

[0028] Description of main component symbols

[0029]

[0030]

[0031] The following specific implementation methods will further illustrate the present application in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0033] It should be noted that when a component is referred to as being "fixed to" or "mounted on" another component, it may be directly on the other component or there may be a central component. When a component is referred to as being "disposed on" another component, it may be directly on the other component or there may be a central component. As used herein, the term "and / or" includes all and any combinations of one or more of the relevant listed items.

[0034] See also Figures 1 to 3The present invention provides an incubation device 100 that can be used to heat, cool, and maintain heat during a biochemical reaction. For example, it can be used to incubate biochemical substances during gene sequencing. "Incubation" refers to creating the required temperature conditions for the biochemical reaction of the biochemical substance by heating, cooling, or maintaining heat. The sample can be, for example, a biological sample (such as a human blood sample, tissue sample, or saliva sample), an adapter, a reagent, or a mixture of two or more thereof, required for biochemical analysis, but is not limited thereto. The incubation device 100 includes a base 1, a heat dissipation module 2 disposed on the base 1, and a heating module 3 disposed on the heat dissipation module 2. The heating module 3 includes a temperature control structure 31 and a heating seat 32 disposed on the temperature control structure 31. The heating seat 32 has at least one heating tank 33. The heating tank 33 can be used to accommodate a container 10 containing a sample. The temperature control structure 31 can regulate the temperature of the heating tank 33 to ensure that the sample in the container 10 reaches a preset temperature, thereby providing the required temperature conditions for the sample's biochemical reaction. Among them, the inner wall 34 of the heating tank 33 has a contoured structure corresponding to the outer wall of the container 10, that is, the shape of the inner wall 34 of the heating tank 33 is designed to be roughly the same as the outer wall shape of the container 10. By contouring the inner wall 34 of the heating tank 33, the inner wall 34 can be more closely fitted to the outer wall of the container 10, increasing the contact area between the heating tank 33 and the container 10, thereby improving the heat conduction efficiency. At the same time, this contouring design will also make the heating of the sample in the container 10 more uniform, which is more conducive to the incubation of the sample.

[0035] like Figure 3 As shown, the base 1 includes a mounting plate 11 and a side wall 12 arranged on the periphery of the mounting plate 11. The heat dissipation module 2 is installed on the mounting plate 11, and the mounting plate 11 is provided with a through hole 13 corresponding to the heat dissipation module 2. The through hole 13 is provided on the mounting plate 11 to facilitate air intake.

[0036] In some embodiments, as Figure 4 As shown, a plurality of openings 14 may be provided on the side wall 12 to facilitate entry of cool air from the outside, thereby further improving heat dissipation efficiency.

[0037] Please refer to Figure 5The heat dissipation module 2 includes a heat dissipation bracket 21 provided on the base 1, and a heat dissipation fan 22 and a radiator 23 provided on the heat dissipation bracket 21. The heat dissipation bracket 21 is installed on the mounting plate 11 of the base 1. Specifically, the heat dissipation bracket 21 is installed on the edge of the mounting plate 11. The bottom plate of the heat dissipation bracket 21 is connected to the through hole 13 of the base 1. The middle part of the heat dissipation bracket 21 forms an accommodating cavity 24. The heat dissipation fan 22 and the radiator 23 are both located in the accommodating cavity 24. The heat dissipation fan 22 is arranged close to the base 1, the radiator 23 is arranged away from the base 1, and the heating module 3 is installed on the radiator 23. The air inlet 25 of the heat dissipation fan 22 faces the base 1, and the air outlet 26 of the heat dissipation fan 22 faces the radiator 23. In this way, the heat dissipation fan 22 can draw in cold air from the outside through the air inlet 25 and discharge it to the radiator 23 from the air outlet 26, thereby taking away the heat of the radiator 23 and achieving the effect of cooling the heating module 3.

[0038] In some embodiments, the heat sink 23 includes a heat sink 27 and a plurality of heat dissipation teeth 28 disposed on the surface of the heat sink 27 facing the cooling fan 22. The heating module 3 is mounted on the heat sink 27. Heat from the heating module 3 is directly transferred to the heat sink 27 and then dissipated through the heat dissipation teeth 28. The plurality of heat dissipation teeth 28 increases the heat dissipation area of ​​the heat sink 23, further improving heat dissipation efficiency and thereby increasing the cooling speed of the heating module 3.

[0039] In some embodiments, the surface of the heat sink 27 close to the heating module 3 is flat, so the heating module 3 can fit more closely to the heat sink 27, thereby further improving the heat conduction efficiency.

[0040] In some embodiments, the heat dissipation teeth 28 and the heat dissipation plate 27 are integrally formed, which can further improve the heat conduction rate.

[0041] In some embodiments, multiple heat dissipation teeth 28 can be arranged roughly in parallel, and an air duct 29 can be formed between two adjacent heat dissipation teeth 28, which is conducive to heat dissipation, especially in conjunction with the design of the heat dissipation fan 22, which is conducive to further improving the heat dissipation efficiency.

[0042] In some embodiments, as Figure 6 As shown, a first heat-conducting layer 6 is provided between the heat sink 23 and the temperature-control structure 31 to further improve heat transfer efficiency. Specifically, the first heat-conducting layer 6 is provided between the heat sink 27 and the temperature-control structure 31 to ensure a closer fit between the heat sink 27 and the temperature-control structure 31, thereby reducing the air gap formed between the heat sink 27 and the temperature-control structure 31. Since the thermal conductivity of the first heat-conducting layer 6 is higher than that of air, the heat transfer efficiency and the cooling rate can be improved.

[0043] In some embodiments, the first heat-conducting layer 6 may be made of a material with high thermal conductivity, such as silver silicone grease, thermal conductive adhesive, etc.

[0044] Please refer to Figure 7 The temperature control structure 31 can be a thermoelectric cooler (or semiconductor cooler, TEC). TECs are quiet, vibration-free, require no refrigerant, are compact, and lightweight. They also offer reliable operation, easy operation, fast cooling and heating, and precise temperature control. The temperature control structure 31 includes a first surface 311 and a second surface 312 positioned opposite each other. The first surface 311 is positioned adjacent to the heating base 32, while the second surface 312 is positioned adjacent to the radiator 23. During heating, the first surface 311 serves as the hot surface, while the second surface 312 serves as the cold surface. During cooling, the first surface 311 serves as the cold surface, while the second surface 312 serves as the hot surface.

[0045] The inner wall 34 of the heating tank 33 is designed to be contoured according to the outer wall shape of the container 10. Figure 7 As shown, the container 10 has a generally conical structure. Therefore, the inner wall 34 of the heating groove 33 is also designed to have a conical structure to more closely fit the outer wall of the container 10. In some embodiments, along the depth direction h of the heating groove 33, the heating groove 33 includes a first groove 36, a second groove 37, and a third groove 371, which are sequentially connected. The inner diameter L1 of the first groove 36 is larger than the inner diameter L2 of the second groove 37 and the inner diameter L3 of the third groove 371. The inner diameter L2 of the second groove 37 decreases as it moves away from the first groove 36, forming a tapered groove structure for the second groove 37. The inner diameter L3 of the third groove 371 is approximately equal to the inner diameter of the end of the second groove 37 away from the first groove 36, so that the sidewall of the third groove 371 is approximately perpendicular to the bottom, and ultimately the first groove 36, the second groove 37, and the third groove 371 form a tapered stepped groove with a reduced diameter. This stepped groove design facilitates the mass production of the heating groove 33. At the same time, this contoured design also makes the heating of the sample in the container 10 more uniform, which is more conducive to the incubation of the sample. It is understood that in other embodiments, the heating groove may also include a first groove and a second groove that are interconnected, wherein the inner diameter of the first groove is larger than the inner diameter of the second groove, and the inner diameter of the second groove decreases in a direction away from the first groove, so that the second groove forms a tapered groove structure. This tapered second groove can better fit the outer wall of the tapered container.

[0046] In some embodiments, according to the tapered shape of the container 10 , the taper of the second groove 37 of the heating groove 33 is substantially the same as the taper of the container 10 .

[0047] The heating seat 32 includes a heating base 321 arranged on the side of the temperature control structure 31 away from the heat dissipation module 2 and at least one heating groove body 322 arranged on the side of the heating base 321 away from the temperature control structure 31. The heating groove 33 is formed on the heating groove body 322. The temperature control structure 31 is clamped between the heating base 321 and the heat dissipation plate 27. By fixing the heating base 321 and the heat dissipation plate 27 together, the temperature control structure 31 can be clamped, thereby making the temperature control structure 31 in close contact with the heat dissipation plate 27 and the heating base 321.

[0048] In some embodiments, the heating base 321 and the heating tank 322 can be an integrally formed structure, which can improve the efficiency of heating and cooling. For example, the heating base 321 is a metal bath made of metal.

[0049] In some embodiments, the heating tank 33 extends to the heating base 321, effectively increasing the depth of the heating tank 33, fully utilizing the space of the heating base 321, and placing the heating tank 33 closer to the temperature control structure 31, further improving heating and cooling efficiency. In this embodiment, the heating tank 33 can accommodate containers 10 with a volume in the milliliter range, thereby effectively increasing the throughput of sample incubation.

[0050] In some embodiments, the distance between the bottom of the heating tank 33 and the surface of the temperature control structure 31 close to the heating base 321 is between 2 mm and 4 mm, which can keep the heating and cooling rate within a reasonable range, so as not to affect the biochemical reaction too fast or too slow to affect the heating and cooling efficiency.

[0051] In some embodiments, the heating base 321 is provided with a plurality of heating slots 322, each of which has a heating slot 33. A gap exists between adjacent heating slots 322, allowing the plurality of heating slots 322 to be independently disposed. This allows the overall heating base 32 to form a hollow structure, thereby reducing the weight of the heating module 3 by approximately 1 / 2 compared to a non-hollow design. Furthermore, the hollow design shortens the heating and cooling time, thereby reducing the energy consumption of the incubation device 100.

[0052] Please refer to Figure 8 and Figure 9When the heating module 3 and the heat dissipation module 2 are installed, the heating base 32 can be directly connected to the heat dissipation plate 27 of the radiator 23. Specifically, steps 38 are provided on opposite sides of the heating base 321, and a first connection hole 39 is provided on the step 38. A heat insulation block 5 is provided on the step 38. A boss 51 is provided on the side of the heat insulation block 5 close to the step 38. The boss 51 can extend into the first connection hole 39. The boss 51 has a second connection hole 52, and the second connection hole 52 is set through the heat insulation block 5. When fixing the heating base 32 on the heat dissipation plate 27, the heat insulation block 5 is placed on the step 38, and the boss 51 is extended into the first connection hole 39. Then, the connecting piece (such as a screw) is passed through the second connection hole 52 of the heat insulation block 5 and the hole on the heat dissipation plate 27 to fix the heating base 321 on the radiator 23. At the same time, due to the connection between the radiator 23 and the heating base 32, the temperature control structure 31 is clamped and fixed in the middle. Since the boss 51 extends into the first connecting hole 39, a certain gap is formed between the connecting member and the hole wall of the first connecting hole 39, so that the connecting member does not directly contact the heating base 321, thereby avoiding heat loss caused by direct contact between the heating member and the heating base 321. The heat insulating block 5 can be made of a heat insulating material.

[0053] In some embodiments, as Figure 6 As shown, a second heat-conducting layer 7 can be provided between the heating base 32 and the temperature-control structure 31 to provide a closer fit between the heating base 32 and the temperature-control structure 31 and reduce the air gap formed between the heating base 32 and the temperature-control structure 31. Since the thermal conductivity of the second heat-conducting layer 7 is higher than that of air, the heat conduction efficiency and the cooling rate can be improved. Specifically, the material of the second heat-conducting layer 7 can be made of the same material as the first heat-conducting layer 6, such as silver silicone grease, thermal adhesive, etc.

[0054] like Figure 3 As shown, the heating module 3 also includes a temperature sensor 35. The temperature sensor 35 is arranged on the heating module 3, specifically on the heating base 321, so as to monitor the temperature changes of the heating seat 32 and the heating tank 33, and feed back the temperature to the controller (not shown) in real time. It has the function of monitoring and feeding back the temperature.

[0055] Please refer again Figures 1 to 3 and Figure 8As shown, the incubation device 100 also includes a heat insulation cover 4 that is sleeved on the outside of the heating module 3. The heat insulation cover 4 wraps the entire heating seat 32, isolates the contact between the air and the heating seat 32, and plays the role of isolating heat radiation and preventing the formation of condensed water. The heat insulation cover 4 is provided with a mounting hole 41 corresponding to the heating groove 33. The side wall of the mounting hole 41 at one end away from the heat dissipation module 2 protrudes toward the inside of the mounting hole 41 to form a protrusion 42. The protrusion 42 is provided corresponding to the end face of the heating groove 33 away from the heat dissipation module 2 to cover the end face of the heating groove 33. Specifically, the protrusion 42 is located at the end of the heating groove body 322 away from the heat dissipation module 2 to isolate the heating module 3 from the outside air to the greatest extent. In addition, when the container 10 is extended into the heating groove 33 from the mounting hole 41, the end of the container 10 can further enhance the effect of isolating the heating groove 33 from the outside world.

[0056] When using, Figure 2 and Figure 3 As shown, a container 10 (e.g., a reagent bottle) containing a sample (e.g., a sample for library preparation) is placed into a heating tank 33. The sample volume is, for example, 1 / 3 to 2 / 3 of the volume of the container 10. At this point, the sidewalls of the container 10 are in contact with the inner wall 34 of the heating tank 33. The bottom of the container 10 is approximately 2 mm to 4 mm from the first surface 311 of the temperature control structure 31. The sample in the container 10 is completely buried in the heating tank 33. The incubation device 100 begins heating. At this point, the first surface 311 of the temperature control structure 31 serves as the hot surface, while the second surface 312 serves as the cold surface. The hot surface of the temperature control structure 31 transfers its temperature to the library preparation sample in the container 10 through the heating base 32. The cold surface of the temperature control structure 31 transfers its temperature to the radiator 23. The cooling fan 22 blows cool air into the air duct 29, thereby dissipating the temperature on the radiator 23. A temperature sensor 8 monitors the temperature changes of the heating base 32 and the heating tank 33. When the temperature reaches the preset temperature, the temperature control structure 31 maintains a constant temperature and the sample begins to be incubated.

[0057] After the set incubation time is reached, the incubation device 100 begins to cool down. At this time, the first surface 311 of the temperature control structure 31 is the cold surface, and the second surface 312 is the hot surface. The cold surface of the temperature control structure 31 transmits the cold surface temperature to the sample contained in the container 10 through the heating seat 32. The hot surface of the temperature control structure 31 transmits the hot surface temperature to the radiator 23, and the cooling fan 22 dissipates heat from the radiator 23. After the set cooling time is reached, the container 10 is removed. When the incubation device 100 of this embodiment completes the preparation of the single-molecule sequencing library, the usual heating temperature can be controlled at 25 to 60°C, which is relatively low, and the amount of library after incubation is large, and no amplification step is required.

[0058] The incubation device 100 provided in the embodiment of the present application, by contouring the inner wall 34 of the heating tank 33, can make the inner wall 34 of the heating tank 33 fit more closely with the outer wall of the container 10, increase the contact area between the heating tank 33 and the container 10, and thus improve the heat conduction efficiency. At the same time, this contouring design also makes the heating of the sample in the container 10 more uniform, which is more conducive to the incubation of the sample; the heating seat 32 adopts a hollow design, which is about 1 / 2 smaller in volume than the non-hollow design, resulting in a smaller heating area, faster heat conduction speed, and effectively shortened heating and cooling time, which is conducive to reducing energy consumption.

[0059] See also Figure 10 The present invention also provides a biochemical reaction system 1000, which may include a biochemical reaction platform 200, wherein the biochemical reaction platform 200 includes the aforementioned incubation device 100. When the biochemical reaction system 1000 is a sequencing system, it may also include a sequencer, but is not limited thereto. In this case, the biochemical reaction platform 200 may be a library preparation instrument, and the biological sample incubated in the incubation device 100 may be transferred to the sequencer for sequencing.

[0060] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application and are not intended to limit the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present application.

Claims

1. An incubation device, characterized in that: include: base; A heat dissipation module is provided on the base; The heating module includes a temperature control structure provided on the heat dissipation module and a heating seat provided on the temperature control structure. The heating seat has at least one heating groove for accommodating a container loaded with a sample. The temperature control structure is used to regulate the temperature of the heating groove so that the sample in the container reaches a preset temperature. The inner wall of the heating groove has a contoured structure corresponding to the outer wall of the container.

2. The incubation device according to claim 1, wherein Along the depth direction of the heating groove, the heating groove includes a first groove and a second groove that are connected to each other, the inner diameter of the first groove is larger than the inner diameter of the second groove, and the inner diameter of the second groove decreases successively in the direction away from the first groove.

3. The incubation device according to claim 1, wherein Along the depth direction of the heating groove, the heating groove includes a first groove, a second groove and a third groove that are connected in sequence. The inner diameter of the first groove is larger than the inner diameters of the second groove and the third groove. The inner diameter of the second groove decreases in the direction away from the first groove. The inner diameter of the third groove is equal to the inner diameter of the end of the second groove away from the first groove.

4. The incubation device according to claim 1, wherein The heating seat includes a heating base and a plurality of heating slots located on a side of the heating base away from the temperature control structure. Each heating slot has a heating slot, and there is a gap between two adjacent heating slots so that the plurality of heating slots are independently arranged.

5. The incubation device according to claim 4, wherein The heating base and the plurality of heating troughs are an integrated structure, and the heating troughs extend to the heating base.

6. The incubation device according to claim 5, wherein The distance between the bottom of the heating tank and the surface of the temperature control structure close to the heating base is 2 mm to 4 mm.

7. The incubation device according to claim 4, wherein Steps are respectively provided on the opposite sides of the heating base, a first connecting hole is provided through the steps, an insulating block is provided on the step, a boss is provided on the side of the insulating block close to the first connecting hole, the boss extends into the first connecting hole, a second connecting hole is provided through the boss, the second connecting hole is provided through the insulating block, and the connecting part is connected to the heat dissipation module by passing through the second connecting hole and the first connecting hole.

8. The incubation device according to claim 1, wherein The incubation device also includes a heat insulation cover arranged on the outside of the heating module, and the heat insulation cover is provided with a mounting hole corresponding to the heating groove. The side wall of the mounting hole at one end away from the heat dissipation module protrudes toward the inside of the mounting hole to form a protrusion, and the protrusion is arranged corresponding to the end surface of the heating groove away from the heat dissipation module to cover the end surface of the heating groove.

9. The incubation device according to claim 1, wherein The heat dissipation module includes a heat dissipation bracket arranged on the base, and a heat dissipation fan and a radiator arranged on the heat dissipation bracket. The temperature control structure is located on the radiator. The heat dissipation fan is located on the side of the radiator away from the heating module. The air inlet of the heat dissipation fan faces the base, and the air outlet of the heat dissipation fan faces the radiator.

10. A biochemical reaction system, characterized in that: include: A biochemical reaction platform and an incubation device according to any one of claims 1 to 9.

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