Nerve-soothing and sleep-aiding yoghurt melting bean and preparation method thereof

Abstract

According to the invention, yoghurt is used as a core raw material, and a nano delivery technology and a flavor regulation and control technology are combined to prepare the composite yoghurt melt with the functions of soothing the nerves and helping sleep. The spina date seed exosome-like particles are extracted and self-assembled with the ganoderan to construct the nanospheres, so that the stability and biocompatibility are improved, the delivery efficiency is also improved, and sleep-aiding effective components are stably released. In addition, a blue butterfly-lactoferrin compound is constructed, the structure of the blue butterfly-lactoferrin compound can mask bitter components, and meanwhile, the blue butterfly-lactoferrin compound has antioxidant and immunomodulatory functions. Rich nutritional ingredients and probiotics in the yoghourt are effectively locked, meanwhile, various functional active ingredients in the spina date seeds, the ganoderma lucidum polysaccharides, the longan, the whey protein and the cynanchum otophyllum are reserved, the multiple effects of soothing the nerves, helping sleep, improving intestinal health and the like are achieved, and the texture and palatability of medicine and food homologous food are remarkably improved.

Description

Technical Field

[0001] The invention belongs to the field of food science and technology, and particularly relates to a soothing and sleep-inducing yogurt dissolving bean and a preparation method thereof. Background Art

[0002] With the accelerated pace of life and increased mental stress in modern society, sleep disorders have become a major issue affecting public health. According to statistics, about 30% of adults worldwide suffer from insomnia symptoms, and traditional solutions mainly rely on benzodiazepines. Chemically synthesized drugs such as jujube seeds and dried longan have been used for a long time, but these drugs are prone to side effects such as dependence and cognitive impairment. In recent years, natural plant extracts (such as jujube seeds and dried longan) have become a research hotspot due to their high safety and calming and heart-nourishing effects. However, these natural active ingredients suffer from poor water solubility, low bioavailability, and easy degradation during processing. For example, jujube seed saponins are easily inactivated at high temperatures or in acidic or alkaline environments, limiting their application in food. Yogurt, a common dairy product, is widely popular for its rich nutritional content and good taste. However, traditional yogurt has limited sleep-inducing properties. In recent years, there has been a trend to enhance the health value of yogurt by adding functional ingredients (such as Chinese jujube seeds and longan). Chinese jujube seeds are a traditional Chinese medicine with significant sedative and hypnotic effects. Its main active ingredient, Chinese jujube saponin A, has been shown to regulate the central nervous system and promote sleep quality. Longan, on the other hand, is rich in polysaccharides and has the effect of nourishing the blood and calming the mind. Currently, there are a variety of sleep-aid products based on Chinese jujube seeds and longans on the market, such as Chinese jujube seed tea, Chinese jujube seed pills, and solid beverages containing Chinese jujube seeds. However, the preparation methods of these products are complex and they are usually based on a single ingredient, lacking synergy with other functional ingredients. In addition, some products suffer from nutrient loss or poor taste during industrial production. Directly adding plant extracts can lead to a bitter taste and unstable ingredients. Against this backdrop, developing a functional product that incorporates natural calming ingredients while retaining the texture and flavor of yogurt-based soy beans has become a pressing technical challenge. Through innovative formulation design and advanced preparation technology, this invention has successfully developed a yogurt-based soy beans that combines calming and sleep-inducing properties with a pleasant taste, providing modern consumers with a safe, effective, and convenient sleep-inducing food option. Summary of the Invention

[0003] Technical Problem to be Solved: Traditional tranquilizing and sleep-inducing products are primarily health foods containing traditional Chinese medicine ingredients, which suffer from poor palatability, low consumer acceptance, and a single ingredient. Yogurt is often used as a carrier due to its nutrient-rich and probiotic properties. This invention, by constructing Ganoderma lucidum polysaccharide-Ziziphus jujuba seed exosome nanospheres and combining them with Blue Butterfly-whey protein complex stabilization technology, uses yogurt as the primary ingredient and achieves a synergistic effect across multiple components. This approach addresses challenges such as processing tolerance and sensory quality balance, resulting in a consumer-friendly sleep-inducing snack that fills a gap in the existing sleep-inducing food market.

[0004] Technical solution: A yogurt-soluble bean for calming the mind and aiding sleep, characterized in that the raw materials, calculated by weight, include: 80-90 parts of yogurt, 3-5 parts of longan pulp, 0.3-0.5 parts of blue butterfly powder, 0.3-0.5 parts of whey protein, and 1-2 parts of Ganoderma lucidum polysaccharide-suanjujube kernel exosome nanospheres. Furthermore, the preparation method of the Ganoderma lucidum polysaccharide-suanzizhi seed exosome nanospheres is as follows: S1. Grind the jujube seeds into powder and mix with buffer, then ultrasonically disrupt to form a homogenate; S2. The homogenate was centrifuged at low speed at 4°C, then at high speed. The supernatant was collected, PBS buffer was added, and ultracentrifuged to obtain a pellet. S3. The pellet was resuspended in PBS buffer and transferred to a sucrose gradient solution. The corresponding bands were collected by ultracentrifugation. The sample was diluted again with PBS buffer and ultracentrifuged again to obtain the jujube seed exosome-like particles. S4. Ganoderma lucidum polysaccharide is dissolved in water to obtain a Ganoderma lucidum polysaccharide solution, and the Ziziphus jujuba seed exosome-like particles are dispersed in water to obtain a Ziziphus jujuba seed exosome-like particle dispersion liquid. The Ziziphus jujuba seed exosome-like particle dispersion liquid and the Ganoderma lucidum polysaccharide solution are fully mixed and the pH is adjusted. High-speed homogenization and emulsification are performed to obtain Ganoderma lucidum polysaccharide-Ziziphus jujuba seed exosome nanospheres. Furthermore, in step S1, the buffer solution is PBS or HEPES buffer solution, the concentration of the powder and the buffer solution is 10-20% (w / v), the frequency of ultrasonic crushing is 800-1000W, and the time is 20-40 min. Furthermore, in step S2, the speed of low-speed centrifugation is 3000-5000g, and the centrifugation time is 10-20 min; the speed of high-speed centrifugation is 8000-10000g, and the centrifugation time is 30-50 min; the speed of ultracentrifugation is 50000-100000g, and the centrifugation time is 40-60 min. Furthermore, in step S3, the sucrose gradient solution is a sucrose solution with a mass percentage of 8%, 15%, 30%, 45% and 60%; the speed of ultracentrifugation is 100,000 to 150,000 g, and the centrifugation is 40 to 60 minutes. Furthermore, in step S4, the concentration of the Ganoderma lucidum polysaccharide solution is 1-3 wt.%, the concentration of the jujube seed exosome-like particle dispersion is 0.5-1 wt.%, and the mass ratio of the jujube seed exosome-like particle dispersion to the Ganoderma lucidum polysaccharide solution is 1:(1-5); the pH is adjusted to 5-6, the speed of the high-speed homogenizer is 10000-12000 rpm, and the time is 5-10 min. A method for preparing yogurt-soluble beans for calming the mind and promoting sleep comprises the following steps: Step 1. preparing a blue butterfly-whey protein complex: dissolving whey protein in PBS buffer to obtain a whey protein solution, adding an aqueous solution of blue butterfly powder to the whey protein solution, adjusting the pH, and ultrasonically treating the solution to obtain a blue butterfly-whey protein complex; Step 2. Mixing and injection molding: Vacuum-mix the yogurt, longan pulp, Ganoderma lucidum polysaccharide-Ziziphus jujuba seed exosome nanospheres, and blue butterfly-whey protein complex, and inject them into the mold; Step 3. Quick freezing and freeze drying: The mold is quick-frozen and then vacuum-frozen and dried. After drying, the yogurt beans are removed from the mold. Furthermore, in step 1, the pH of the PBS buffer solution is 6.5-7.0; the concentration of the whey protein solution is 1-2 wt.%, the concentration of the blue butterfly powder aqueous solution is 1-2 wt.%, the ratio is (1-2):1, and the pH is adjusted to 8-9; the ultrasonic treatment conditions are a power of 100-200 W and a time of 10-15 min. Furthermore, the quick freezing temperature in step 3 is set to -40 to -50°C, and the time is 1.5 to 2 hours; the vacuum freeze drying conditions are a cold trap temperature of -40 to -50°C, a vacuum pressure of 30 to 50 Pa, a plate temperature of 30 to 50°C, and a time of 15 to 20 hours. Beneficial effects: 1. Chinese jujube seeds are rich in fatty oils, proteins, sterols, jujube saponins, vitamin C and other beneficial ingredients. Among them, jujube saponins have significant sedative and hypnotic effects. Chinese jujube seed exosome-like particles are enriched with biologically active substances unique to Chinese jujube seeds (such as saponins, flavonoids, cyclic peptides, etc.). These components may enhance their stability and bioavailability through exosome delivery. The active ingredients carried (such as flavonoids) may enhance the ability to scavenge free radicals. They also have the ability to affect human cells through molecules such as miRNA, such as regulating sleep-related neurotransmitters to achieve a calming effect. 2. The combination of jujube seed exosome-like particles and Ganoderma lucidum polysaccharide not only improves the solubility and biocompatibility, but the addition of the polysaccharide matrix can also significantly improve the physical and mechanical properties of the exosome particles, such as elasticity and toughness, thereby enhancing their stability in complex biological environments. In addition, the addition of the polysaccharide matrix can reduce the immunogenicity of the exosome particles. 3. The Ganoderma lucidum polysaccharide used in the present invention has the function of regulating the hypothalamic-pituitary-adrenal axis (HPA axis), can reduce the level of stress hormones, and at the same time enhance the sensitivity of γ-aminobutyric acid (GABA) receptors, thereby alleviating neural excitability. After combining with the exosome-like particles of the Chinese date seed, it can form nanoparticles with antioxidant and neuromodulatory functions, improve delivery efficiency, realize the regulation of the release rate and targeting of the active ingredients, and further prolong the deep sleep time. 4. The present invention prepares a blue butterfly-whey protein complex, which not only has antioxidant, immunomodulatory, and iron absorption-promoting effects, but may also have positive effects on beauty, improving intestinal health, and enhancing vision. In addition, the tertiary structure of lactoferrin in whey protein has a hydrophobic cavity, which may wrap bitter molecules (such as saponins or flavonoid alkaloids in Chinese jujube seeds) through hydrophobic interactions, thereby masking the bitter taste; the polyphenol structure of anthocyanins in blue butterflies may combine with alkaloids through hydrogen bonds or π-π stacking, helping lactoferrin to more stably wrap bitter molecules and enhance the overall flavor of the product. 5. The present invention achieves synergistic enhancement of multiple components through innovative formula design and preparation process. Jujube seed saponins, Ganoderma lucidum polysaccharides and blue butterfly anthocyanins enhance the calming effect by regulating neurotransmitter, antioxidant and sedative pathways respectively. The sleep-aiding probiotics in yogurt will further enhance the calming and sleep-inducing function. The present invention also achieves synergistic effects of multiple active ingredients through the nanoscale composite of Ganoderma lucidum polysaccharides and jujube seed exosome-like particles (Ganoderma lucidum polysaccharide-jujube seed exosome nanospheres), combined with the stabilized combination of blue butterfly anthocyanins and lactoferrin (blue butterfly-whey protein complex). 6. This invention balances and optimizes sensory quality and functionality. The bitter taste of the jujube kernel is reduced by replacing the jujube kernel with exosome-like particles from the jujube kernel. The blue butterfly gives the dissolved beans a natural light blue color, enhancing their visual appeal, and no artificial coloring is added. The low-temperature quick freezing and vacuum freeze-drying technology are used to maximize the activity of probiotics and the efficacy of heat-sensitive ingredients, while improving the palatability of the product. 7. All ingredients of this invention are derived from natural food materials, without any chemical synthetic additives. Food-grade carrier technology is used to reduce risks. It is suitable for a variety of people and provides a non-drug-dependent sleep improvement solution. The product has an excellent flavor and taste, and has both market competitiveness and social benefits. BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 This is a sample picture of yogurt dissolving beans; Figure 2 This is the data chart of the overall sleep-aiding effect in the yogurt bean sleep-aiding experiment; Figure 3 Figure 1 shows the test results of shortening the time it takes to fall asleep in a sleep-aiding experiment using yogurt-soluble beans. Figure a shows the test results after the subjects consumed the yogurt-soluble beans for 10 days, and Figure b shows the test results after the subjects consumed the yogurt-soluble beans for 15 days. Figure 4 These are the test results of extending sleep time in the yogurt dissolving beans sleep aid experiment. Figure a shows the test results of the subjects eating yogurt dissolving beans for 10 days, and Figure b shows the test results of the subjects eating yogurt dissolving beans for 15 days. DETAILED DESCRIPTION The present invention will be further described below in conjunction with examples, which are provided to explain the present invention and are not limited to the following examples: Example 1 A yogurt-soluble bean for calming the mind and promoting sleep, comprising the following raw materials in parts by weight: 80 parts of yogurt, 3 parts of longan pulp, 0.3 parts of blue butterfly powder, 0.3 parts of whey protein, and 1 part of ganoderma lucidum polysaccharide-suanjujube kernel exosome nanospheres. The preparation method of Ganoderma lucidum polysaccharide-Ziziphus jujuba seed exosome nanospheres is as follows: S1. Grind the seeds of the Chinese date palm into a powder, take 1 g of the powder and mix it with 10 mL of PBS buffer (pH 6.5), and ultrasonically disrupt it at 1000 W for 20 min to form a homogenate. S2. The homogenate was centrifuged at 3000 g for 20 min at 4°C, then at 8000 g for 30 min. The supernatant was collected, PBS buffer was added, and the pellet was pelleted by ultracentrifugation at 50,000 g for 60 min. S3. The pellet was resuspended in PBS buffer. 6 mL of the resuspended sample, 6 mL of 60% sucrose solution, 6 mL of 45% sucrose solution, 6 mL of 30% sucrose solution, 4 mL of 15% sucrose solution, and 8 mL of 8% sucrose solution were added to the bottom of a transparent ultracentrifuge tube. The tube was ultracentrifuged at 100,000 g for 60 min. The band between 30% and 45% sucrose solution was collected, diluted again with PBS buffer, and ultracentrifuged at 150,000 g for 40 min to obtain the jujube seed exosome-like particles. S4. Ganoderma lucidum polysaccharide was dissolved in water to prepare a 1 wt.% Ganoderma lucidum polysaccharide solution. Ziziphus jujuba seed exosome-like particles were dispersed in water to prepare a 0.5 wt.% Ziziphus jujuba seed exosome-like particle dispersion. The Ziziphus jujuba seed exosome-like particle dispersion and the Ganoderma lucidum polysaccharide solution were thoroughly mixed at a ratio of 1:2, the pH was adjusted to 5, and high-speed homogenization and emulsification were performed at 10,000 rpm for 10 minutes to obtain Ganoderma lucidum polysaccharide-Ziziphus jujuba seed exosome nanospheres. The preparation method of the soothing and sleep-inducing yogurt-soluble beans is as follows: Step 1. Preparation of a blue butterfly-whey protein complex: 0.3 parts of whey protein was dissolved in PBS (pH 6.5) buffer to obtain a 1 wt.% whey protein solution, 2 wt.% blue butterfly powder aqueous solution was added to the whey protein solution at a ratio of 1:1, the pH was adjusted to 8, and the solution was sonicated at a power of 200 W for 10 minutes to obtain a blue butterfly-whey protein complex; Step 2. Mixing and injection molding: Mix yogurt, longan pulp, Ganoderma lucidum polysaccharide-jujube seed exosome nanospheres and blue butterfly-whey protein complex in proportion, stir evenly under vacuum, and inject into the mold; Step 3. Quick freezing and freeze drying: The mold is quick-frozen for 2 hours, the quick freezing temperature is set to -40°C, and then vacuum freeze drying is performed. The conditions for vacuum freeze drying are a cold trap temperature of -40°C, a vacuum pressure of 30Pa, a plate temperature of 30°C, and a time of 15 hours. After drying is completed, the yogurt beans are demolded to obtain the yogurt beans. Example 2 A yogurt-soluble bean for calming the mind and promoting sleep, comprising the following raw materials in parts by weight: 90 parts of yogurt, 3 parts of longan pulp, 0.3 parts of blue butterfly powder, 0.3 parts of whey protein, and 1 part of ganoderma lucidum polysaccharide-suanjujube kernel exosome nanospheres. The preparation method of Ganoderma lucidum polysaccharide-Ziziphus jujuba seed exosome nanospheres is as follows: S1. Grind the seeds of the Chinese date palm into a powder, take 1 g of the powder and mix it with 10 mL of PBS buffer (pH 6.5), and ultrasonically disrupt it at 1000 W for 20 min to form a homogenate. S2. The homogenate was centrifuged at 3000 g for 20 min at 4°C, then at 8000 g for 30 min. The supernatant was collected, PBS buffer was added, and the pellet was pelleted by ultracentrifugation at 50,000 g for 60 min. S3. The pellet was resuspended in PBS buffer. 6 mL of the resuspended sample, 6 mL of 60% sucrose solution, 6 mL of 45% sucrose solution, 6 mL of 30% sucrose solution, 4 mL of 15% sucrose solution, and 8 mL of 8% sucrose solution were added to the bottom of a transparent ultracentrifuge tube. The tube was ultracentrifuged at 100,000 g for 60 min. The band between 30% and 45% sucrose solution was collected, diluted again with PBS buffer, and ultracentrifuged at 150,000 g for 40 min to obtain the jujube seed exosome-like particles. S4. Ganoderma lucidum polysaccharide was dissolved in water to prepare a 1 wt.% Ganoderma lucidum polysaccharide solution. Ziziphus jujuba seed exosome-like particles were dispersed in water to prepare a 0.5 wt.% Ziziphus jujuba seed exosome-like particle dispersion. The Ziziphus jujuba seed exosome-like particle dispersion and the Ganoderma lucidum polysaccharide solution were thoroughly mixed at a ratio of 1:2, the pH was adjusted to 5, and high-speed homogenization and emulsification were performed at 10,000 rpm for 10 minutes to obtain Ganoderma lucidum polysaccharide-Ziziphus jujuba seed exosome nanospheres. The preparation method of the soothing and sleep-inducing yogurt-soluble beans is as follows: Step 1. Preparation of a blue butterfly-whey protein complex: 0.3 parts of whey protein was dissolved in PBS (pH 6.5) buffer to obtain a 1 wt.% whey protein solution, 2 wt.% blue butterfly powder aqueous solution was added to the whey protein solution at a ratio of 1:1, the pH was adjusted to 8, and the solution was sonicated at a power of 200 W for 10 minutes to obtain a blue butterfly-whey protein complex; Step 2. Mixing and injection molding: Mix yogurt, longan pulp, Ganoderma lucidum polysaccharide-jujube seed exosome nanospheres and blue butterfly-whey protein complex in proportion, stir evenly under vacuum, and inject into the mold; Step 3. Quick freezing and freeze drying: The mold is quick-frozen for 2 hours, the quick freezing temperature is set to -40°C, and then vacuum freeze drying is performed. The conditions for vacuum freeze drying are a cold trap temperature of -40°C, a vacuum pressure of 30Pa, a plate temperature of 30°C, and a time of 15 hours. After drying is completed, the yogurt beans are demolded to obtain the yogurt beans. Example 3 A yogurt-soluble bean for calming the mind and promoting sleep, comprising the following raw materials in parts by weight: 90 parts of yogurt, 3 parts of longan pulp, 0.3 parts of blue butterfly powder, 0.3 parts of whey protein, and 2 parts of Ganoderma lucidum polysaccharide-suanjujube kernel exosome nanospheres. The preparation method of Ganoderma lucidum polysaccharide-Ziziphus jujuba seed exosome nanospheres is as follows: S1. Grind the seeds of the Chinese date palm into a powder, take 1 g of the powder and mix it with 10 mL of PBS buffer (pH 6.5), and ultrasonically disrupt it at 1000 W for 20 min to form a homogenate. S2. The homogenate was centrifuged at 3000 g for 20 min at 4°C, then at 8000 g for 30 min. The supernatant was collected, PBS buffer was added, and the pellet was pelleted by ultracentrifugation at 50,000 g for 60 min. S3. The pellet was resuspended in PBS buffer. 6 mL of the resuspended sample, 6 mL of 60% sucrose solution, 6 mL of 45% sucrose solution, 6 mL of 30% sucrose solution, 4 mL of 15% sucrose solution, and 8 mL of 8% sucrose solution were added to the bottom of a transparent ultracentrifuge tube. The tube was ultracentrifuged at 100,000 g for 60 min. The band between 30% and 45% sucrose solution was collected, diluted again with PBS buffer, and ultracentrifuged at 150,000 g for 40 min to obtain the jujube seed exosome-like particles. S4. Ganoderma lucidum polysaccharide was dissolved in water to prepare a 1 wt.% Ganoderma lucidum polysaccharide solution. Ziziphus jujuba seed exosome-like particles were dispersed in water to prepare a 0.5 wt.% Ziziphus jujuba seed exosome-like particle dispersion. The Ziziphus jujuba seed exosome-like particle dispersion and the Ganoderma lucidum polysaccharide solution were thoroughly mixed at a ratio of 1:2, the pH was adjusted to 5, and high-speed homogenization and emulsification were performed at 10,000 rpm for 10 minutes to obtain Ganoderma lucidum polysaccharide-Ziziphus jujuba seed exosome nanospheres. The preparation method of the soothing and sleep-inducing yogurt-soluble beans is as follows: Step 1. Preparation of a blue butterfly-whey protein complex: 0.3 parts of whey protein was dissolved in PBS (pH 6.5) buffer to obtain a 1 wt.% whey protein solution, 2 wt.% blue butterfly powder aqueous solution was added to the whey protein solution at a ratio of 1:1, the pH was adjusted to 8, and the solution was sonicated at a power of 200 W for 10 minutes to obtain a blue butterfly-whey protein complex; Step 2. Mixing and injection molding: Mix yogurt, longan pulp, Ganoderma lucidum polysaccharide-jujube seed exosome nanospheres and blue butterfly-whey protein complex in proportion, stir evenly under vacuum, and inject into the mold; Step 3. Quick freezing and freeze drying: The mold is quick-frozen for 2 hours, the quick freezing temperature is set to -40°C, and then vacuum freeze drying is performed. The conditions for vacuum freeze drying are a cold trap temperature of -40°C, a vacuum pressure of 30Pa, a plate temperature of 30°C, and a time of 15 hours. After drying is completed, the yogurt beans are demolded to obtain the yogurt beans. Comparative Example 1 The difference between this comparative example and Example 3 is that the same amount of jujube seed powder and Ganoderma lucidum polysaccharide are used instead of Ganoderma lucidum polysaccharide-jujube seed exosome nanospheres, as follows: The invention discloses a yogurt-soluble bean for calming the mind and promoting sleep. The raw materials thereof include, calculated by weight, 90 parts of yogurt, 3 parts of longan pulp, 0.3 parts of blue butterfly powder, 0.3 parts of whey protein, 0.4 parts of jujube kernel powder, and 1.6 parts of ganoderma lucidum polysaccharide. The preparation method of yogurt-soluble beans for calming the mind and aiding sleep is as follows: Step 1. Preparation of a blue butterfly-whey protein complex: 0.3 parts of whey protein was dissolved in PBS (pH 6.5) buffer to obtain a 1 wt.% whey protein solution, 2 wt.% blue butterfly powder aqueous solution was added to the whey protein solution at a ratio of 1:1, the pH was adjusted to 8, and the solution was sonicated at a power of 200 W for 10 minutes to obtain a blue butterfly-whey protein complex; Step 2. Mixing and injection molding: Mix yogurt, longan pulp, Ganoderma lucidum polysaccharide, jujube seed powder and blue butterfly-whey protein complex in proportion, stir evenly under vacuum, and inject into the mold; Step 3. Quick freezing and freeze drying: The mold is quick-frozen for 2 hours, the quick freezing temperature is set to -40°C, and then vacuum freeze drying is performed. The conditions for vacuum freeze drying are a cold trap temperature of -40°C, a vacuum pressure of 30Pa, a plate temperature of 30°C, and a time of 15 hours. After drying is completed, the yogurt beans are demolded to obtain the yogurt beans. Comparative Example 2 The difference between this comparative example and Example 3 is that the blue butterfly powder is not compounded with whey protein, specifically as follows: A yogurt-soluble bean for calming the mind and promoting sleep, comprising the following raw materials in parts by weight: 90 parts of yogurt, 3 parts of longan pulp, 0.3 parts of blue butterfly powder, 0.3 parts of whey protein, and 2 parts of Ganoderma lucidum polysaccharide-suanjujube kernel exosome nanospheres. The preparation method of Ganoderma lucidum polysaccharide-Ziziphus jujuba seed exosome nanospheres is as follows: S1. Grind the seeds of the Chinese date palm into a powder, take 1 g of the powder and mix it with 10 mL of PBS buffer (pH 6.5), and ultrasonically disrupt it at 1000 W for 20 min to form a homogenate. S2. The homogenate was centrifuged at 3000 g for 20 min at 4°C, then at 8000 g for 30 min. The supernatant was collected, PBS buffer was added, and the pellet was pelleted by ultracentrifugation at 50,000 g for 60 min. S3. The pellet was resuspended in PBS buffer. 6 mL of the resuspended sample, 6 mL of 60% sucrose solution, 6 mL of 45% sucrose solution, 6 mL of 30% sucrose solution, 4 mL of 15% sucrose solution, and 8 mL of 8% sucrose solution were added to the bottom of a transparent ultracentrifuge tube. The tube was ultracentrifuged at 100,000 g for 60 min. The band between 30% and 45% sucrose solution was collected, diluted again with PBS buffer, and ultracentrifuged at 150,000 g for 40 min to obtain the jujube seed exosome-like particles. S4. Ganoderma lucidum polysaccharide was dissolved in water to prepare a 1 wt.% Ganoderma lucidum polysaccharide solution. Ziziphus jujuba seed exosome-like particles were dispersed in water to prepare a 0.5 wt.% Ziziphus jujuba seed exosome-like particle dispersion. The Ziziphus jujuba seed exosome-like particle dispersion and the Ganoderma lucidum polysaccharide solution were thoroughly mixed at a ratio of 1:2, the pH was adjusted to 5, and high-speed homogenization and emulsification were performed at 10,000 rpm for 10 minutes to obtain Ganoderma lucidum polysaccharide-Ziziphus jujuba seed exosome nanospheres. The preparation method of the soothing and sleep-inducing yogurt-soluble beans is as follows: Step 1. Mixing and injection molding: Mix yogurt, longan pulp, Ganoderma lucidum polysaccharide-jujube seed exosome nanospheres, blue butterfly powder, and whey protein in proportion, stir evenly under vacuum, and inject into the mold; Step 2. Quick freezing and freeze drying: The mold is quick-frozen for 2 hours, the quick freezing temperature is set to -40°C, and then vacuum freeze drying is performed. The conditions for vacuum freeze drying are a cold trap temperature of -40°C, a vacuum pressure of 30Pa, a plate temperature of 30°C, and a time of 15 hours. After drying is completed, the yogurt beans are demolded to obtain the yogurt beans. Indicator testing (1) Sensory evaluation Fifteen food-related professional sensory evaluators with no special taste preferences were asked to objectively evaluate the finished products based on the sensory evaluation form (see Table 1). Table 1 Sensory evaluation standards The sensory evaluation results are shown in Table 2. The examples are superior to the comparative examples in terms of smell and taste. The yogurt content in Example 2 is increased, and the smell and taste are better, while the comparative example is poor in taste. Comparative Example 1 adds sour jujube seed powder, which has a bitter taste and may affect the overall flavor. Comparative Example 2 may be related to the bitterness not being completely encapsulated. Table 2 Sensory evaluation results (2) Sleep aid test analysis The subjects were organized to eat yogurt-soluble beans at regular intervals and in fixed quantities. The age range of the subjects was 18 to 45 years old. According to the sleep quality standard, they filled in information. 150 subjects were selected on a voluntary basis. The subjects were divided into three groups based on the data. The final effective number of subjects in Group A (taking Example 3) was 50, the final effective number of subjects in Group B (taking Comparative Example 1) was 50, and the final effective number of subjects in Group C (taking Comparative Example 2) was 50. Each course of treatment was 15 days. After the end of the course of treatment, the effect was evaluated in a timely manner, and the improvement in daily sleep time, sleep onset time, dreaminess, early awakening / awakening, and daytime status was recorded. The subjects did not experience any adverse reactions or dependence after taking the drug. The subjects' overall feelings about the invention were investigated. The results are as follows: Figure 2 As shown. All three groups of subjects showed improvement in sleep problems, including sleep duration, sleep onset time, frequent dreams, and awakenings, indicating that the yogurt-soluble beans, a medicinal and edible ingredient, can improve sleep problems in subjects. Group A, which consumed Example 3, showed the most significant improvement, while Group B, which consumed Control Example 1, showed less improvement. This may be due to the lack of Ganoderma lucidum polysaccharide-suanzizhi seed exosome nanospheres. Although suanzizhi seed powder can help with sleep, it is difficult to achieve this effect in the short term. The effects of soothing and sleep-inducing yogurt beans on sleep time were investigated, and the subjects' sleep onset time and sleep extension time were counted. The results are as follows Figure 3 and 4 shown. like Figure 3 After consuming the yogurt-soluble beans, the subjects' sleep time was significantly improved. Figure 3 In Figure a, after consuming the yogurt-soluble beans for 10 days, 32% of the subjects in Group A, which consumed Example 3, had their sleep time shortened by more than 1 hour, while only 14% and 24% of the subjects in Groups B and C, which consumed the control sample, had their sleep time shortened by more than 1 hour respectively. As the drinking time increased, the subjects' sleep time was significantly shortened, as shown in Figure 4. Figure 3 Figure b shows that after the 15th day, the sleep time of 3 subjects in Group A who consumed Example 3 was shortened by 2 to 3 hours, and the sleep problems of 40 out of 50 subjects were significantly improved. The sleep problems of 34 subjects in Group B who consumed Control Example 1 were improved, indicating that it has a sleep-inducing effect, but the sleep-inducing effect is not as good as that of the embodiment. like Figure 4 After eating yogurt beans, the subjects' sleep time increased significantly. Figure 4 After consuming the drink for 10 days, nearly 50% of the people in Group A who consumed Example 3 had their sleep time extended, 18 people in Group B who consumed Comparative Example 1 had their sleep time extended, and 21 people in Group C who consumed Comparative Example 2 had their sleep time extended. As the drinking time increased, more and more subjects' sleep time improved, as shown in Figure 1. Figure 4Figure b shows that by the 15th day, 56% of the subjects in Group A had significantly improved their sleep, and 10 of them had their sleep time extended by more than 2 hours; after consuming the product for 15 days (e.g. Figure 4 (Figure b), 42% and 50% of the subjects in Group B and Group C, respectively, had prolonged sleep time. In summary, consuming the sleep-inducing and soothing yogurt beans can significantly prolong the subjects' sleep time. The data show that the embodiment has a better soothing effect. The above description is merely a preferred embodiment of the present invention and does not constitute any form of limitation to the present invention. Any person skilled in the art can, without departing from the spirit and technical solution of the present invention, use the methods and technical contents disclosed above to make many possible changes and modifications to the technical solution of the present invention, or modify it into an equivalent embodiment with equivalent changes. Therefore, any simple modification, equivalent replacement, equivalent change and modification made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention shall still fall within the scope of protection of the technical solution of the present invention.

Claims

1. A soothing and sleep-inducing yogurt bean paste, characterized in that: Calculated by weight, the raw materials include: 80-90 parts of yogurt, 3-5 parts of longan pulp, 0.3-0.5 parts of blue butterfly powder, 0.3-0.5 parts of whey protein, and 1-2 parts of Ganoderma lucidum polysaccharide-jujube kernel exosome nanospheres.

2. The soothing and sleep-inducing yogurt-soluble bean according to claim 1, characterized in that: The preparation method of the Ganoderma lucidum polysaccharide-suanzizhi seed exosome nanospheres is as follows: S1. Grind the jujube seeds into powder and mix with buffer solution, then ultrasonically disrupt them to form a homogenate; S2. The homogenate was centrifuged at low speed at 4°C, then at high speed. The supernatant was collected, PBS buffer was added, and ultracentrifuged to obtain a pellet. S3. The pellet was resuspended in PBS buffer and transferred to a sucrose gradient solution. The corresponding bands were collected by ultracentrifugation. The sample was diluted again with PBS buffer and ultracentrifuged again to obtain the jujube seed exosome-like particles. S4. Ganoderma lucidum polysaccharide is dissolved in water to obtain a Ganoderma lucidum polysaccharide solution, and the Ziziphus jujuba seed exosome-like particles are dispersed in water to obtain a Ziziphus jujuba seed exosome-like particle dispersion liquid. The Ziziphus jujuba seed exosome-like particle dispersion liquid and the Ganoderma lucidum polysaccharide solution are fully mixed and the pH is adjusted. High-speed homogenization and emulsification are performed to obtain Ganoderma lucidum polysaccharide-Ziziphus jujuba seed exosome nanospheres.

3. The soothing and sleep-inducing yogurt-soluble bean according to claim 2, characterized in that: In step S1, the buffer solution is PBS or HEPES buffer solution, the concentration of the powder and the buffer solution is 10-20% (w / v), the frequency of ultrasonic crushing is 800-1000W, and the time is 20-40 minutes.

4. The soothing and sleep-inducing yogurt-soluble bean according to claim 2, characterized in that: In step S2, the low-speed centrifugation is performed at a speed of 3000-5000 g for 10-20 min; the high-speed centrifugation is performed at a speed of 8000-10000 g for 30-50 min; and the ultracentrifugation is performed at a speed of 50000-100000 g for 40-60 min.

5. The soothing and sleep-inducing yogurt-soluble bean according to claim 2, characterized in that: In step S3, the sucrose gradient solution is a sucrose solution with a mass percentage of 8%, 15%, 30%, 45% and 60%; the speed of ultracentrifugation is 100,000-150,000 g, and the centrifugation is 40-60 minutes.

6. The soothing and sleep-inducing yogurt-soluble bean according to claim 2, characterized in that: In step S4, the concentration of the Ganoderma lucidum polysaccharide solution is 1-3 wt.%, the concentration of the Suanzigous jujube seed exosome-like particle dispersion is 0.5-1 wt.%, and the mass ratio of the Suanzigous jujube seed exosome-like particle dispersion to the Ganoderma lucidum polysaccharide solution is 1:(1-5); the pH is adjusted to 5-6, the speed of the high-speed homogenizer is 10000-12000 rpm, and the time is 5-10 min.

7. The method for preparing the soothing and sleep-inducing yogurt-soluble beans according to any one of claims 1 to 6, characterized in that: The following steps are involved: Step 1. preparing a blue butterfly-whey protein complex: dissolving whey protein in PBS buffer to obtain a whey protein solution, adding an aqueous solution of blue butterfly powder to the whey protein solution, adjusting the pH, and ultrasonically treating the solution to obtain a blue butterfly-whey protein complex; Step 2. Mixing and injection molding: Vacuum-mix the yogurt, longan pulp, Ganoderma lucidum polysaccharide-Ziziphus jujuba seed exosome nanospheres, and blue butterfly-whey protein complex, and inject them into the mold; Step 3. Quick freezing and freeze drying: The mold is quick-frozen and then vacuum-frozen and dried. After drying, the yogurt beans are removed from the mold.

8. The method for preparing the soothing and sleep-inducing yogurt-soluble beans according to claim 7, characterized in that: In the step 1, the pH value of the PBS buffer solution is 6.5-7.0; the concentration of the whey protein solution is 1-2 wt.%, the concentration of the blue butterfly powder aqueous solution is 1-2 wt.%, the ratio is (1-2):1, and the pH value is adjusted to 8-9; the ultrasonic treatment conditions are a power of 100-200 W and a time of 10-15 min.

9. The method for preparing the soothing and sleep-inducing yogurt-soluble beans according to claim 7, characterized in that: The quick freezing temperature in step 3 is set to -40 to -50°C and the time is 1.5 to 2 hours; the vacuum freeze drying conditions are a cold trap temperature of -40 to -50°C, a vacuum pressure of 30 to 50 Pa, a plate temperature of 30 to 50°C, and a time of 15 to 20 hours.

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