Continuous fermentation device for D-lactic acid

Abstract

The invention relates to the technical field of lactic acid fermentation, and provides a D-lactic acid continuous fermentation device which comprises an equipment shell and a fermentation chamber formed in the equipment shell and used for continuously fermenting lactic acid, a supporting column is fixedly installed in the fermentation chamber, and a spherical connecting block is fixedly installed on the supporting column; a connecting platform is rotatably mounted on the spherical connecting block, a culture dish is mounted on the connecting platform in an inserted manner, and a lifting device for enabling liquid in the culture dish to flow is mounted in the equipment shell. When a motor is started to enable a limiting plate to drive a roller to move to an ascending chute and a descending chute respectively, the ascending chute and the descending chute can enable a sliding plate to move upwards or downwards respectively and enable a connecting platform to rotate upwards or downwards around a spherical connecting block; the fermentation liquid continuously flows back and forth between the two fermentation tanks through the flowing channel, so that the condition that the local PH concentration is relatively high or relatively low due to non-uniform mixing of a culture medium and the fermentation liquid is avoided.

Description

Technical Field

[0001] The invention relates to the technical field of lactic acid fermentation, in particular to a continuous fermentation device for D-lactic acid. Background Art

[0002] D-lactic acid is an optical isomer of lactic acid, an enantiomer of the more common L-lactic acid. Its molecular formula contains a chiral carbon atom, and its name derives from the right-handed orientation of the hydroxyl group in the Fischer projection. D-lactic acid is found in relatively small quantities in nature, but its unique chemical and biological properties have attracted considerable attention in recent years in the fields of industry, medicine, and materials science.

[0003] Since continuous fermentation involves the continuous addition of culture medium and harvesting of products, while batch fermentation involves a one-time addition and harvesting after fermentation, the advantages of continuous fermentation include higher production efficiency, more stable product quality, and reduced downtime. pH control during fermentation is particularly important because the accumulation of lactic acid lowers the pH of the culture medium and inhibits bacterial growth. When culture medium is added, the culture medium covers the surface of the fermentation liquid, so the fermentation liquid at the bottom receives less culture medium, resulting in locally high or low pH concentrations and a reduced acid production rate. Summary of the Invention

[0004] To address the shortcomings of the prior art, the present invention provides a continuous fermentation device for D-lactic acid, which solves the problem of uneven mixing of culture medium and fermentation liquid resulting in high or low local pH concentration.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A continuous fermentation device for D-lactic acid comprises an equipment housing and a fermentation chamber provided therein for continuous fermentation of lactic acid. A support column is fixedly mounted in the fermentation chamber, a spherical connecting block is fixedly mounted on the support column, a connecting platform is rotatably mounted on the spherical connecting block, a culture dish is plugged and mounted on the connecting platform, a lifting device is mounted in the equipment housing for allowing liquid in the culture dish to flow, and as the lifting device moves, the culture dish rotates up and down around the spherical connecting block, a mixing device is mounted on the culture dish for accelerating the fermentation of the fermentation liquid, and a driving device is mounted on the equipment housing.

[0007] Material toggling mechanism, its both sides respectively have a cylinder pressure, and the cylinder pressure bar connects swing arm, and the swing arm end face has hook portion, and a bar passes position between the end of two swing arms and the hook portion.

[0008] Preferably, the lifting device also includes two groups of fermentation tanks opened on the culture dish, the fermentation tanks are installed with inclined plates, the culture dish is provided with a flow channel connected to the two groups of fermentation tanks, the culture dish is installed with two groups of baffles, and the culture dish is plugged and installed with a pH sensor.

[0009] Preferably, the mixing device comprises two sets of tooth plates mounted on an H-shaped sliding frame, four sets of No. 1 rotating blocks are rotatably mounted on the connecting platform, and each set of No. 1 rotating blocks is mounted with an outer tooth ring that meshes with the tooth plates.

[0010] Preferably, four groups of No. 2 rotating blocks used in conjunction with the No. 1 rotating block are rotatably mounted on the culture dish, and a sealing rod is fixedly mounted on each group of the No. 2 rotating blocks, and two groups of stirring paddles are mounted on the sealing rod.

[0011] Preferably, a plug-in device is installed on the No. 1 rotating block, and the plug-in device includes an inserting block fixedly installed on the No. 1 rotating block, and a slot for use with the inserting block is opened on the No. 2 rotating block.

[0012] Preferably, multiple groups of No. 1 magnetic blocks are fixedly installed on the culture dish, and multiple groups of connection slots for use with No. 1 magnetic blocks are provided on the connection platform, and each group of the connection slots is fixedly installed with No. 2 magnetic blocks attracted to No. 1 magnetic blocks.

[0013] Preferably, the driving device includes a motor fixedly mounted on the device housing, and a reciprocating screw threadedly connected to the limiting plate is fixedly mounted on the output end of the motor.

[0014] Preferably, a sealing door is rotatably mounted on the device housing.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] 1. The present invention adopts the arrangement of a lifting device and a driving device. When the motor is started to drive the limit plate to move the roller to the ascending chute and the descending chute respectively, the ascending chute and the descending chute will respectively move the sliding plate upward or downward and cause the connecting platform to rotate upward or downward around the spherical connecting block, so that the fermentation liquid continuously flows back and forth between the two fermentation tanks through the flow channel, thereby avoiding the situation where the local pH concentration is higher or lower due to uneven mixing of the culture medium and the fermentation liquid.

[0017] 2. The present invention provides a mixing device. When the H-shaped sliding frame moves, it drives the tooth plate to move. The movement of the tooth plate drives the No. 1 rotating block connected to the outer gear ring to rotate. Under the action of the plug-in device, the rotation of the No. 1 rotating block drives the No. 2 rotating block to rotate. The rotation of the No. 2 rotating block drives the stirring paddle connected to the sealing rod to rotate. When the fermentation liquid is on the inclined plate and the flow channel, it will exceed the stirring range, so that the fermentation liquid in the fermentation tank can be properly stirred, thereby effectively improving the yield of D-lactic acid continuous fermentation.

[0018] 3. The present invention utilizes a plug-in device to install the culture dish by aligning the plug-in block with the slot and then aligning the No. 1 magnetic block on the culture dish with the No. 2 magnetic block on the connecting slot so that they attract each other. This ensures that the position of the plug-in block and the interface are consistent, thereby preventing the culture dish from shifting during installation and accommodating culture dishes of different sizes, thereby increasing the range of use of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0020] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0021] Figure 2 This is a schematic diagram of the overall structure of the culture dish of the present invention;

[0022] Figure 3 This is a schematic diagram of the overall structure inside the housing of the device of the present invention;

[0023] Figure 4 This is an exploded schematic diagram of the connection platform of the present invention;

[0024] Figure 5 This is a schematic diagram of the explosion of the culture dish of the present invention;

[0025] Figure 6 This is a schematic diagram of the positional relationship between the second rotating block and the slot of the present invention.

[0026] Figure: 1, equipment housing; 2, fermentation chamber; 3, support column; 301, ball-type connecting block; 302, connecting platform; 303, culture dish; 4, lifting device; 401, sliding trough; 402, ascending chute; 403, descending chute; 404, stopping platform; 405, fixing rod; 406, limit plate; 407, sliding plate; 408, counterweight; 409, roller; 410, H-shaped sliding frame; 411, fixing block; 412, fermentation tank; 413, inclined Plate; 414, flow channel; 415, baffle; 416, pH sensor; 5, mixing device; 501, tooth plate; 502, rotating block No. 1; 503, outer gear ring; 504, rotating block No. 2; 505, sealing rod; 506, stirring paddle; 6, plug-in device; 601, plug-in block; 602, slot; 603, magnetic block No. 1; 604, connecting groove; 605, magnetic block No. 2; 7, driving device; 701, motor; 702, reciprocating screw; 8, sealing door. DETAILED DESCRIPTION

[0027] The following will describe the implementation methods of the present application in detail with reference to the accompanying drawings and examples, so that the implementation process of how the present application applies technical means to solve technical problems and achieve technical effects can be fully understood and implemented accordingly.

[0028] Example 1

[0029] When adding culture medium, the culture medium covers the surface of the fermentation liquid. The fermentation liquid at the bottom will get less culture medium, which will cause the local pH concentration to be higher or lower, and reduce the acid production rate. If the fermentation liquid is continuously stirred, it may cause the cell activity to decrease and even lead to the death of the bacteria. In order to solve this problem, refer to Figures 1-6 This embodiment provides a continuous fermentation device for D-lactic acid. By setting a lifting device 4, the problem of uneven mixing of culture medium and fermentation liquid, which leads to high or low local pH concentration, is solved. The device includes an equipment housing 1 and a fermentation chamber 2 for continuous fermentation of lactic acid. A sealing door 8 is rotatably mounted on the equipment housing 1. A support column 3 is fixedly mounted in the fermentation chamber 2. A spherical connecting block 301 is fixedly mounted on the support column 3. A connecting platform 302 is rotatably mounted on the spherical connecting block 301. A culture dish 303 is plugged and mounted on the connecting platform 302. When the culture dish 303 is in use, the opening on the culture dish 303 is covered with a sealing cover to ensure the sealing of the culture dish 303. A lifting device 4 is installed in the device housing 1 to allow the liquid in the culture dish 303 to flow. As the lifting device 4 moves, the culture dish 303 rotates up and down around the spherical connecting block 301. As the culture dish 303 rotates up and down, the fermentation liquid in the culture dish 303 continuously flows and is evenly mixed with the culture medium. A mixing device 5 is installed on the culture dish 303 to accelerate the fermentation of the fermentation liquid. Through proper stirring, the yield of continuous D-lactic acid fermentation can be improved.

[0030] The lifting device 4 includes a sliding groove 401 provided on the equipment housing 1, an ascending chute 402 is provided on the sliding groove 401, a descending chute 403 connected to the ascending chute 402 is provided on the sliding groove 401, a stopping platform 404 is provided between the descending chute 403 and the ascending chute 402, when the roller 409 is in the position of the stopping platform 404, the culture dish 303 is in a horizontal state, two groups of fixed rods 405 are fixedly installed on the sliding groove 401, and a limiting plate 406 slidably connected to the fixed rod 405 is slidably installed on the sliding groove 401. Under the action of the fixed rod 405, the movement of the limiting plate 406 is more stable, and two groups of sliding plates 407 are slidably installed on the limiting plate 406, and the sliding plate 407 is fixedly installed A counterweight block 408 is provided to pull the culture dish 303 to rotate downward, a roller 409 is rotatably mounted on the counterweight block 408, an H-shaped sliding frame 410 is slidably mounted on the connecting platform 302, and a fixed block 411 rotatably connected to the sliding plate 407 is fixedly mounted on the H-shaped sliding frame 410. When the limit plate 406 drives the roller 409 to move to the ascending chute 402 and the descending chute 403 respectively, the ascending chute 402 and the descending chute 403 can respectively make the sliding plate 407 move upward or downward and make the connecting platform 302 rotate upward or downward around the spherical connecting block 301, so that the fermentation liquid in the culture dish 303 continuously flows back and forth in the two fermentation tanks 412. The lifting device 4 also includes a hole provided on the culture dish 303. 3, for the continuous fermentation of D-lactic acid, the fermentation tank 412 is provided with an inclined plate 413, the culture dish 303 is provided with a flow channel 414 connected to the two fermentation tanks 412, the culture dish 303 is provided with two sets of baffles 415, the culture dish 303 is plugged with a pH sensor 416, which is convenient for pH detection of the fermentation liquid, and the flowing fermentation liquid will cause the pH detection measurement position to change continuously, and the result is more accurate. A driving device 7 is installed on the device housing 1, and the driving device 7 includes a motor 701 fixedly mounted on the device housing 1, and a reciprocating screw rod 702 threadedly connected to the limit plate 406 is fixedly mounted on the output end of the motor 701. Under the action of the plug-in device 6, the The culture dish 303 is limited on the connecting platform 302. In the initial state, the roller 409 is located at the stop platform 404, and the culture dish 303 is in a horizontal state. The motor 701 is started to make the reciprocating screw 702 at the output end rotate slowly in the limiting plate 406, so that the limiting plate 406 drives the sliding plate 407 to reciprocate in the concave of the sliding groove 401. The sliding plate 407 drives the roller 409 connected to the counterweight block 408 and the H-shaped sliding frame 410 connected to the fixed block 411 to move. When the roller 409 moves to the rising chute 402, the sliding plate 407 moves upward, and the sliding plate 407 lifts the connecting platform 302 connected to the H-shaped sliding frame 410, and the connecting platform 302 rotates upward around the spherical connecting block 301.This allows the fermentation liquid in the fermentation tank 412 farther from the spherical connecting block 301 to flow into the other fermentation tank. Conversely, when the roller 409 moves to the descending chute 403, the connecting platform 302 rotates downward around the spherical connecting block 301, allowing the fermentation liquid to continuously flow between the two fermentation tanks 412 through the flow channel 414, thus avoiding the situation where the pH concentration is locally high or low due to uneven mixing of the culture medium and the fermentation liquid.

[0031] Example 2

[0032] Since continuous stirring of the fermentation liquid may cause bacterial death in severe cases, moderate stirring can improve the mixing efficiency and alleviate product inhibition by promoting flow, which can increase the yield of continuous D-lactic acid fermentation. In order to solve this problem, refer to Figures 1-6 The mixing device 5 includes two sets of tooth plates 501 mounted on the H-shaped sliding frame 410, four sets of No. 1 rotating blocks 502 are rotatably mounted on the connecting platform 302, and each set of No. 1 rotating blocks 502 is equipped with an outer gear ring 503 that meshes with the tooth plates 501. Four sets of No. 2 rotating blocks 504 that cooperate with the No. 1 rotating blocks 502 are rotatably mounted on the culture dish 303, and each set of No. 2 rotating blocks 504 is fixedly mounted with a sealing rod 505, and two sets of stirring paddles 506 are installed on the sealing rod 505. The H-shaped sliding frame 410 moves When the gear plate 501 is moved, the gear plate 501 drives the No. 1 rotating block 502 connected to the outer gear ring 503 to rotate. Under the action of the plug-in device 6, the No. 1 rotating block 502 rotates, driving the No. 2 rotating block 504 to rotate. The No. 2 rotating block 504 rotates and drives the stirring paddle 506 connected to the sealing rod 505 to rotate. When the fermentation liquid is on the inclined plate 413 and the flow channel 414, it will exceed the stirring range, so that the fermentation liquid in the fermentation tank 412 can be properly stirred, thereby effectively improving the yield of the continuous fermentation of D-lactic acid.

[0033] Example 3

[0034] Since the continuous fermentation of D-lactic acid requires different sizes of culture dishes according to the fermentation time, the methods of installing culture dishes of different sizes are also different. It will take more time to install culture dishes of different sizes. In order to solve this problem, refer to Figures 1-6, a plug-in device 6 is installed on the No. 1 rotating block 502, and the plug-in device 6 includes an insert block 601 fixedly installed on the No. 1 rotating block 502, and a slot 602 for use with the insert block 601 is provided on the No. 2 rotating block 504. A plurality of groups of No. 1 magnetic blocks 603 are fixedly installed on the culture dish 303, and a plurality of groups of connecting slots 604 for use with the No. 1 magnetic block 603 are provided on the connecting platform 302. A No. 2 magnetic block 605 attracted to the No. 1 magnetic block 603 is fixedly installed on each group of connecting slots 604. The insert block 601 is aligned with the slot 602 and inserted, and the No. 1 magnetic block 603 on the culture dish 303 is aligned with the No. 2 magnetic block 605 on the connecting slot 604 and inserted so that they are attracted to each other, thereby completing the installation of the culture dish 303. Through the consistency of the position of the insert block 601 and the interface, not only can the culture dish 303 be prevented from being offset during installation, but also culture dishes 303 of different sizes can be adapted, thereby improving the range of use of the device.

[0035] Working principle: Align the plug 601 with the slot 602 and insert it, and align the No. 1 magnetic block 603 on the culture dish 303 with the No. 2 magnetic block 605 on the connecting groove 604 so that they attract each other, thereby completing the installation of the culture dish 303, starting the motor 701 so that the reciprocating screw rod 702 at the output end rotates slowly in the limit plate 406, so that the limit plate 406 drives the sliding plate 407 to reciprocate in the concave of the sliding groove 401, and the sliding plate 407 drives the roller 409 connected to the counterweight block 408 and the H-shaped sliding frame 410 connected to the fixed block 411 to move. When the roller 409 moves to the rising inclined groove 402, the sliding plate 407 moves upward, and the sliding plate 407 lifts the connecting platform 302 connected to the H-shaped sliding frame 410, and the connecting platform 302 rotates upward around the spherical connecting block 301 so that the distance from the spherical The fermentation liquid in the fermentation tank 412 farther away from the connecting block 301 flows into the other fermentation liquid. Conversely, when the roller 409 moves to the descending chute 403, the connecting platform 302 rotates downward around the spherical connecting block 301, so that the fermentation liquid continuously flows between the two fermentation tanks 412 through the flow channel 414. When the H-shaped sliding frame 410 moves, it drives the tooth plate 501 to move. The movement of the tooth plate 501 drives the No. 1 rotating block 502 connected to the outer gear ring 503 to rotate. Under the action of the plug-in device 6, the No. 1 rotating block 502 rotates and drives the No. 2 rotating block 504 to rotate. The No. 2 rotating block 504 rotates and drives the stirring paddle 506 connected to the sealing rod 505 to rotate. When the fermentation liquid is on the inclined plate 413 and the flow channel 414, it will exceed the stirring range, so that the fermentation liquid in the fermentation tank 412 can be properly stirred.

[0036] It should be noted that, in this article, the terms "comprises", "includes" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or apparatus that includes a series of elements includes not only those elements, but also includes other elements not explicitly listed, or also includes elements that are inherent to such process, method, article or apparatus.

[0037] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A continuous fermentation device for D-lactic acid, comprising a device housing (1) and a fermentation chamber (2) provided therein for continuous fermentation of lactic acid, characterized in that: A support column (3) is fixedly installed in the fermentation chamber (2), a spherical connection block (301) is fixedly installed on the support column (3), a connection platform (302) is rotatably installed on the spherical connection block (301), a culture dish (303) is plugged and installed on the connection platform (302), a lifting device (4) for allowing the liquid in the culture dish (303) to flow is installed in the device housing (1), and as the lifting device (4) moves, the culture dish (303) rotates up and down around the spherical connection block (301), a mixing device (5) for accelerating the fermentation of the fermentation liquid is installed on the culture dish (303), and a driving device (7) is installed on the device housing (1).

2. The continuous fermentation device for D-lactic acid according to claim 1, characterized in that: The lifting device (4) comprises a sliding groove (401) provided on the equipment housing (1), an ascending chute (402) provided on the sliding groove (401), a descending chute (403) connected to the ascending chute (402) provided on the sliding groove (401), a stop platform (404) provided between the descending chute (403) and the ascending chute (402), two groups of fixed rods (405) fixedly installed on the sliding groove (401), a limiting plate (406) slidably connected to the fixed rod (405) slidably installed on the sliding groove (401), two groups of sliding plates (407) slidably installed on the limiting plate (406), and the sliding plates (407) ) is fixedly mounted with a counterweight block (408), a roller (409) is rotatably mounted on the counterweight block (408), an H-shaped sliding frame (410) is slidably mounted on the connecting platform (302), a fixed block (411) rotatably connected to the sliding plate (407) is fixedly mounted on the H-shaped sliding frame (410), and when the limiting plate (406) drives the roller (409) to move to the ascending chute (402) and the descending chute (403) respectively, the ascending chute (402) and the descending chute (403) can respectively enable the sliding plate (407) to move upward or downward and enable the connecting platform (302) to rotate upward or downward around the spherical connecting block (301).

3. The continuous fermentation device for D-lactic acid according to claim 2, characterized in that: The lifting device (4) further comprises two groups of fermentation tanks (412) provided on the culture dish (303), an inclined plate (413) being installed on the fermentation tanks (412), a flow channel (414) being provided on the culture dish (303) and communicating with the two groups of fermentation tanks (412), two groups of baffles (415) being installed on the culture dish (303), and a pH sensor (416) being plugged and installed on the culture dish (303).

4. The continuous fermentation device for D-lactic acid according to claim 3, characterized in that: The mixing device (5) comprises two sets of tooth plates (501) mounted on an H-shaped sliding frame (410), four sets of No. 1 rotating blocks (502) are rotatably mounted on the connecting platform (302), and each set of No. 1 rotating blocks (502) is mounted with an outer tooth ring (503) that meshes with the tooth plates (501).

5. The continuous fermentation device for D-lactic acid according to claim 4, characterized in that: Four groups of No. 2 rotating blocks (504) used in conjunction with the No. 1 rotating block (502) are rotatably mounted on the culture dish (303), and a sealing rod (505) is fixedly mounted on each group of the No. 2 rotating blocks (504), and two groups of stirring paddles (506) are mounted on the sealing rods (505).

6. The continuous fermentation device for D-lactic acid according to claim 5, characterized in that: The first rotating block (502) is provided with a plug-in device (6), the plug-in device (6) comprising an inserting block (601) fixedly mounted on the first rotating block (502), and the second rotating block (504) is provided with a slot (602) for use with the inserting block (601).

7. The continuous fermentation device for D-lactic acid according to claim 1, characterized in that: The culture dish (303) is fixedly mounted with a plurality of groups of No. 1 magnetic blocks (603), and the connecting platform (302) is provided with a plurality of groups of connecting slots (604) for use with the No. 1 magnetic blocks (603). Each group of the connecting slots (604) is fixedly mounted with a No. 2 magnetic block (605) that is attracted to the No. 1 magnetic block (603).

8. The continuous fermentation device for D-lactic acid according to claim 1, characterized in that: The driving device (7) comprises a motor (701) fixedly mounted on the device housing (1), and a reciprocating screw (702) threadedly connected to a limit plate (406) is fixedly mounted on the output end of the motor (701).

9. The continuous fermentation device for D-lactic acid according to claim 1, characterized in that: A sealing door (8) is rotatably mounted on the equipment housing (1).

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