High multiplex digital nucleic acid assay using melting temperature
By amplifying target nucleic acids with thermal barcoded primers and identifying them using melting curves, the limitations of multiple amplification and barcoding in traditional methods are overcome, efficient and simple multiple nucleic acid detection is achieved, and detection throughput is improved.
CN120677252APending Publication Date: 2025-09-19PRESIDENT & FELLOWS OF HARVARD COLLEGE
Patent Information
- Application Number
- CN202480011734.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2023-01-27
- Filing Date
- 2024-01-12
- Publication Date
- 2025-09-19
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Figure CN120677252A_ABST
Abstract
Methods, systems, and articles of manufacture are provided for multiplex assays of target nucleic acids using thermal barcodes. In some cases, the thermal barcodes may be used with one or more signaling entities for improving multiplex assays. At least some of the methods described herein can be performed at relatively high throughput without the need for expensive and slow nucleic acid sequencing. In some cases, these methods may be implemented using a system or article comprising a plurality of droplets containing a target nucleic acid.
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Citation Information
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