Toxin-expelling and bowel-relaxing enzyme based on pitaya and prebiotics and preparation method of toxin-expelling and bowel-relaxing enzyme

Abstract

The invention discloses a toxin-expelling and bowel-relaxing enzyme based on pitaya and prebiotics and a preparation method thereof, and relates to the technical field of enzyme preparation, and the toxin-expelling and bowel-relaxing enzyme comprises the following raw materials in percentage: 70-80% of red pitaya fermented puree, 8-12% of isomaltooligosacharide, 0.05-0.1% of composite strains, 0.1-0.2% of vitamin C and the balance of purified water. According to the invention, natural components such as red pitaya fermented puree and isomaltooligosacharide are used as raw materials, and processes such as segmented compounding, precise temperature control anaerobic fermentation, high-pressure homogenization and ultrahigh-pressure sterilization are carried out, so that a dual-action mechanism of'physical excretion promotion of dietary fibers and synergistic regulation of prebiotics and probiotics' is formed; risks of melanosis coli, drug dependence and the like caused by the fact that a traditional irritant laxative destroys intestinal nerve plexus are avoided, and toxin expelling and bowel relaxing efficiency is remarkably improved.

Description

Technical Field

[0001] The present invention relates to the technical field of enzyme preparation, in particular to a detoxifying and laxative enzyme based on pitaya and prebiotics and a preparation method thereof. Background Art

[0002] In modern society, the fast-paced lifestyle is reshaping people's daily routines. High-pressure work from nine to five, frequent late-night overtime work, and long-term lack of exercise have gradually weakened intestinal motility. At the same time, high-fat and high-salt takeout fast food and over-processed convenience foods dominate the dining table, and dietary fiber intake is seriously insufficient. The combination of these two factors has led to constipation becoming a common problem that plagues some adults. The accumulation of intestinal toxins is closely related to sub-health conditions such as acne, bad breath, and decreased immunity. Faced with huge health needs.

[0003] However, in the existing technology, the market for traditional detoxification and laxative products was once prosperous, and stimulant laxatives such as senna and phenolphthalein were widely used because of their quick effect. However, clinical studies have shown that long-term use of such products will damage the intestinal nerve plexus, leading to an increased risk of colon melanosis. Some users develop drug dependence, forming a vicious cycle of "having to have laxatives". Summary of the Invention

[0004] The purpose of the present invention is to provide a detoxifying and laxative enzyme based on pitaya and prebiotics and a preparation method thereof, so as to solve the problems raised by the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solution: a detoxifying and laxative enzyme based on pitaya and prebiotics, comprising the following raw materials in the following percentages: 70-80% fermented pitaya pulp, 8-12% isomaltooligosaccharide, 0.05-0.1% composite bacteria, 0.1-0.2% vitamin C, and the remainder being purified water; the pH value of the fermented pitaya pulp is 3.8-4.2, and the number of viable bacteria is ≥10 7 CFU / mL, anthocyanin content ≥20mg / 100mL; isomaltooligosaccharide purity ≥95%, 2-5 degree of polymerization ratio ≥80%; the composite bacteria consists of Lactobacillus acidophilus and Bifidobacterium BB-12.

[0006] Preferably, the preparation method of the detoxifying laxative enzyme based on pitaya and prebiotics comprises the following steps: S1. Raw material pretreatment: The fermented pitaya pulp is processed through a double filter and a disc separator to obtain a clarified pulp liquid, which is temporarily stored under controlled temperature; oligomeric isomalto-oligosaccharide is prepared into a 50% concentration solution, which is filtered to remove impurities; S2. Segmented compounding and primary fermentation: After mixing the clarified raw pulp with a 5% by weight isomaltooligosaccharide solution, inoculate the pre-activated composite bacteria and ferment in an anaerobic fermentation tank at 37°C ± 0.5°C for 24 hours; S3. Secondary compounding and functional enhancement: After the fermentation is completed, filter and add 3% by weight of isomaltooligosaccharide solution and 0.1% of vitamin C, stir and mix, and then homogenize under high pressure of 120 MPa; S4. Sterilization and filling: After ultra-high pressure sterilization, the prepared liquid is quickly cooled and aseptically filled in a Class A clean area.

[0007] Preferably, during a fermentation process, the pH value and dissolved oxygen content are monitored every 2 hours by a DCS control system to maintain the pH value at 3.8-4.0 and the oxygen content at <0.5%.

[0008] Preferably, the ultrahigh pressure sterilization condition is 600 MPa, maintaining the pressure for 3 minutes, and rapidly cooling to below 25° C. after sterilization.

[0009] Preferably, the anaerobic fermentation tank comprises a fermentation mechanism, the upper part of the fermentation mechanism is fixedly connected to a feeding mechanism and an inert gas introduction mechanism, and the sides of the fermentation mechanism are fixedly connected to a temperature regulating mechanism and a mixing mechanism, the fermentation mechanism comprises a water bath, the interior of the water bath is fixedly connected to a fermentation tank body, the mixing mechanism is used to stir the raw materials inside the fermentation tank body, a discharge pipe is fixedly connected to the bottom of the fermentation tank body, a No. 1 valve is installed on the surface of the discharge pipe, a drain pipe is fixedly connected to the bottom of the water bath, a No. 2 valve is installed on the surface of the drain pipe, the temperature regulating mechanism is used to regulate the fermentation temperature inside the fermentation tank body, the inert gas introduction mechanism is used to regulate the fermentation oxygen concentration inside the water bath, and a No. 2 temperature sensor and a pH sensor are respectively installed inside the fermentation tank body.

[0010] Preferably, the temperature regulating mechanism includes a No. 1 circulation pump, a cold water inlet pipe and a heating assembly, a No. 1 connecting pipe and a No. 2 connecting pipe are respectively installed at the ends of the No. 1 circulation pump, the No. 1 connecting pipe is fixedly connected to the bottom of the water bath, the No. 2 connecting pipe is fixedly connected to the temperature conducting pipe, the temperature conducting pipe is fixedly connected to the inner wall of the water bath, and the bottom of the temperature conducting pipe is fixedly connected to a temperature conducting platform, a plurality of temperature conducting fins are provided on the surface of the temperature conducting platform, a No. 3 connecting pipe is fixedly connected to the side of the temperature conducting pipe, the No. 3 connecting pipe is located on the outside of the fermentation tank body, the cold water inlet pipe is fixedly connected to the water bath, and a No. 3 valve is installed on the surface of the cold water inlet pipe, the cold water inlet pipe is externally connected to a cold water supply device, the heating assembly is fixedly connected to the water bath, and a No. 1 temperature sensor is fixedly connected to the surface of the water bath.

[0011] Preferably, the mixing mechanism includes a No. 2 circulation pump, a discharge pipe, a connecting shaft, a water wheel and a material guiding platform. The ends of the No. 2 circulation pump are respectively installed with a No. 4 connecting pipe and a No. 5 connecting pipe. The No. 4 connecting pipe is fixedly connected to the fermentation tank body, the No. 5 connecting pipe is fixedly connected to the discharge pipe, the connecting shaft is rotatably connected to the top of the water bath, and the connecting shaft is fixedly connected to the water wheel. The discharge pipe is located above the fermentation tank body, and a plurality of discharge nozzles located in the same horizontal plane and distributed in a ring are installed on the inner side of the discharge pipe. The discharge nozzles are inclined at an angle toward the direction of the blades of the water wheel. A through hole is opened at the bottom of the water wheel, and the through hole is located above the temperature guiding platform. A stirring rod is installed on the surface of the connecting shaft, and the stirring rod is located inside the fermentation tank body. The material guiding platform is fixedly connected to the connecting shaft, and a plurality of material distribution plates in a ring array are fixedly connected to the surface of the material guiding platform.

[0012] Preferably, the feeding mechanism includes a feeding pipe, which is fixedly connected to the water bath and is located above the fermentation tank body. A No. 4 valve is installed on the surface of the feeding pipe. The inert gas introduction mechanism includes an air inlet pipe and a dissolved oxygen sensor. The air inlet pipe is fixedly connected to the water bath and a No. 5 valve is installed on the surface of the air inlet pipe. The dissolved oxygen sensor is installed on the upper part of the water bath, and the air inlet pipe is externally connected to the inert gas supply equipment.

[0013] Compared with the prior art, the present invention has the following beneficial effects: In the present invention, red pitaya fermented pulp, isomaltooligosaccharide and other natural ingredients are used as raw materials, and through processes such as segmented compounding, precise temperature-controlled anaerobic fermentation, high-pressure homogenization and ultra-high pressure sterilization, a dual action mechanism of "physical promotion of excretion by dietary fiber + synergistic regulation of prebiotics and probiotics" is formed. This not only avoids the risks of traditional irritant laxatives damaging the intestinal nerve plexus, causing colon melanosis and drug dependence, but also significantly improves the detoxification and laxative efficiency. At the same time, the fermentation environment is strictly controlled by the DCS control system, and ultra-high pressure sterilization technology is used to retain the activity of the effective ingredients to the greatest extent, ensuring stable product quality and achieving natural, efficient and safe detoxification and laxative effects. In the present invention, the feeding mechanism and the inert gas introduction mechanism respectively realize the input of raw materials and the regulation of oxygen concentration. The temperature regulating mechanism uses the water bath, the heating component and the No. 2 temperature sensor to accurately control the temperature in the fermentation tank body to maintain it at the set value. The mixing mechanism fully stirs the raw materials in the fermentation tank body to ensure uniform fermentation. The discharge pipe and the No. 1 valve, the water discharge pipe and the No. 2 valve facilitate the discharge of materials and the replacement of the water bath water. The pH sensor in the fermentation tank body monitors the reaction acidity and alkalinity in real time. The close cooperation of these parts not only provides a stable environment for anaerobic fermentation, but also ensures that the fermentation process is efficient and sufficient, effectively improving product quality and fermentation efficiency. In the present invention, a water circulation loop is formed by a No. 1 circulation pump, a No. 1 connecting pipe, a No. 2 connecting pipe, a temperature conducting pipe and a No. 3 connecting pipe to continuously stir the water in the water bath to avoid excessive local temperature differences caused by cold water injection or heating, thereby ensuring uniform and stable water temperature; the cold water inlet pipe, the No. 3 valve and the external cold water supply equipment cooperate to quickly inject cold water to cool down the fermentation tank body when the temperature is too high, and the heating component accurately heats up when the temperature is too low; the No. 1 temperature sensor monitors the water bath temperature in real time to provide data support for regulation; the temperature conducting pipe, the temperature conducting platform and the temperature conducting fin plate form an efficient heat conduction structure to enable the raw materials flowing through to quickly contact the appropriate temperature, thereby reducing the risk of damage to the activity of the bacterial community or death near the inner wall of the fermentation tank body due to sudden temperature changes, and through the dual mechanism of water bath temperature control and rapid heat exchange, the temperature regulation accuracy and stability are significantly improved, ensuring that the fermentation process is efficient and stable; In the present invention, the No. 2 circulation pump in the mixing mechanism pumps the raw materials in the fermentation tank body to the discharge pipe through the No. 4 connecting pipe and the No. 5 connecting pipe, and the raw materials are sprayed out at high speed through the inclined discharge nozzle to impact the water wheel blades, driving the connecting shaft and the stirring rod to rotate. The raw materials are mixed in multiple stages under the triple effects of the pump body, the jet impact and the stirring rod; the through hole at the bottom of the water wheel cooperates with the guide platform and the dividing plate to evenly disperse the fallen raw materials into the fermentation tank body, thereby enhancing the mixing effect. Inert gas is introduced into the mechanism, and the dissolved oxygen sensor monitors the oxygen concentration in the water bath in real time. When the oxygen concentration exceeds the standard, inert gas is injected through the air inlet pipe and the No. 5 valve to quickly replace the air and maintain an anaerobic environment. The two mechanisms are linked to achieve efficient stirring without an additional power source through the principle of fluid mechanics, and accurately control the fermentation conditions with the help of intelligent sensing and gas replacement technology, thereby significantly improving the fermentation uniformity and product quality stability. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a flow chart of the detoxifying and laxative enzyme based on pitaya and prebiotics and the preparation method thereof of the present invention; Figure 2 This is a first three-dimensional structural diagram of the anaerobic fermentation tank in the detoxification and laxative enzyme based on pitaya and prebiotics and the preparation method thereof of the present invention; Figure 3 This is a second three-dimensional structural diagram of the anaerobic fermentation tank in the detoxification and laxative enzyme based on pitaya and prebiotics and the preparation method thereof of the present invention; Figure 4 This is a three-dimensional structural diagram of the water bath tank inside the anaerobic fermentation tank in the detoxification and laxative enzyme based on pitaya and prebiotics and the preparation method thereof of the present invention; Figure 5 This is a three-dimensional structural diagram of the fermentation tank body of the anaerobic fermentation tank in the detoxification and laxative enzyme based on pitaya and prebiotics and the preparation method thereof of the present invention; Figure 6This is a cross-sectional front view of the anaerobic fermentation tank used in the detoxifying and laxative enzyme based on pitaya and prebiotics and the preparation method thereof of the present invention; Figure 7 This is a three-dimensional structural diagram of the discharge pipe of the anaerobic fermentation tank in the detoxification and laxative enzyme based on pitaya and prebiotics and the preparation method thereof of the present invention; Figure 8 This is a front view of the cross-section of the fermentation tank body of the anaerobic fermentation tank in the detoxification and laxative enzyme based on pitaya and prebiotics and the preparation method thereof of the present invention; Figure 9 This is a three-dimensional structural diagram of the water wheel of the anaerobic fermentation tank in the detoxification and laxative enzyme based on pitaya and prebiotics and the preparation method thereof of the present invention.

[0015] Legend: 1. Fermentation mechanism; 11. Water bath; 12. Fermentation tank body; 13. Discharge pipe; 14. Valve No. 1; 15. Drain pipe; 16. Valve No. 2; 2. Temperature control mechanism; 21. Circulation pump No. 1; 22. Connecting pipe No. 1; 23. Connecting pipe No. 2; 24. Temperature conduction pipe; 25. Connecting pipe No. 3; 26. Temperature conduction platform; 27. Temperature conduction fin plate; 28. Cold water inlet pipe; 29. ​​Valve No. 3; 210. Heating assembly; 3. Mixing mechanism; 31 , No. 2 circulation pump; 32. No. 4 connecting pipe; 33. No. 5 connecting pipe; 34. Discharge pipe; 35. Discharge nozzle; 36. Connecting shaft; 37. Water wheel; 38. Stirring rod; 39. Material guide table; 310. Material distribution plate; 4. Feeding mechanism; 41. Feeding pipe; 42. No. 4 valve; 5. Inert gas introduction mechanism; 51. Inlet pipe; 52. No. 5 valve; 53. Dissolved oxygen sensor; 6. No. 1 temperature sensor; 7. No. 2 temperature sensor; 8. pH sensor. DETAILED DESCRIPTION

[0016] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the implementation regulations described are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0017] The present invention will be further described below with reference to the embodiments.

[0018] Example 1: Please refer to Figure 1 As shown, a detoxifying laxative enzyme based on pitaya and prebiotics contains the following raw materials: 70-80% fermented pitaya puree, 8-12% isomaltooligosaccharide, 0.05-0.1% composite bacteria, 0.1-0.2% vitamin C, and the balance is purified water; the pH value of the fermented pitaya puree is 3.8-4.2, and the number of viable bacteria is ≥107 CFU / mL, anthocyanin content ≥20mg / 100mL; isomaltooligosaccharide purity ≥95%, 2-5 degree of polymerization ratio ≥80%; the composite bacteria consists of Lactobacillus acidophilus and Bifidobacterium BB-12.

[0019] The preparation method of a detoxifying and laxative enzyme based on pitaya and prebiotics comprises the following steps: S1. Raw material pretreatment: The fermented pitaya pulp is processed through a double filter and a disc separator to obtain a clarified pulp liquid, which is temporarily stored under controlled temperature; oligomeric isomalto-oligosaccharide is prepared into a 50% concentration solution, which is filtered to remove impurities; S2. Segmented compounding and primary fermentation: After mixing the clarified raw pulp with a 5% by weight isomaltooligosaccharide solution, inoculate the pre-activated composite bacteria and ferment in an anaerobic fermentation tank at 37°C ± 0.5°C for 24 hours; S3. Secondary compounding and functional enhancement: After the fermentation is completed, filter and add 3% by weight of isomaltooligosaccharide solution and 0.1% of vitamin C, stir and mix, and then homogenize under high pressure of 120 MPa; S4. Sterilization and filling: After ultra-high pressure sterilization, the prepared liquid is quickly cooled and aseptically filled in a Class A clean area.

[0020] During a fermentation process, the pH value and dissolved oxygen content are monitored every 2 hours through the DCS control system to maintain the pH value at 3.8-4.0 and the oxygen content at <0.5%. The ultra-high pressure sterilization conditions are 600MPa, the pressure is maintained for 3 minutes, and the temperature is quickly lowered to below 25°C after sterilization.

[0021] In this embodiment, red pitaya fermented pulp, oligosaccharides and other natural ingredients are used as raw materials, and through processes such as segmented compounding, precise temperature-controlled anaerobic fermentation, high-pressure homogenization and ultra-high pressure sterilization, a dual action mechanism of "physical promotion of excretion by dietary fiber + synergistic regulation of prebiotics and probiotics" is formed. This not only avoids the risks of traditional irritant laxatives destroying intestinal nerve plexus, causing colon melanosis and drug dependence, but also significantly improves the efficiency of detoxification and laxative. At the same time, the fermentation environment is strictly controlled by the DCS control system, and ultra-high pressure sterilization technology is used to retain the activity of effective ingredients to the greatest extent, ensuring stable product quality and achieving natural, efficient and safe detoxification and laxative effects.

[0022] Example 2: Figure 2-Figure 9As shown, the anaerobic fermentation tank includes a fermentation mechanism 1, the upper part of the fermentation mechanism 1 is fixedly connected to a feeding mechanism 4 and an inert gas introduction mechanism 5, and the side of the fermentation mechanism 1 is fixedly connected to a temperature regulating mechanism 2 and a mixing mechanism 3, the fermentation mechanism 1 includes a water bath 11, the interior of the water bath 11 is fixedly connected to a fermentation tank body 12, the mixing mechanism 3 is used to stir the raw materials inside the fermentation tank body 12, the bottom of the fermentation tank body 12 is fixedly connected to a discharge pipe 13, the surface of the discharge pipe 13 is installed with a No. 1 valve 14, the bottom of the water bath 11 is fixedly connected to a drain pipe 15, the surface of the drain pipe 15 is installed with a No. 2 valve 16, the temperature regulating mechanism 2 is used to adjust the fermentation temperature inside the fermentation tank body 12, the inert gas introduction mechanism 5 is used to adjust the fermentation oxygen concentration inside the water bath 11, and the fermentation tank body 12 is respectively installed with a No. 2 temperature sensor 7 and a pH sensor 8.

[0023] In this embodiment, water is injected into the water bath 11, and the water is heated to the set fermentation temperature by the heating component 210. The raw materials are fed into the fermentation tank body 12 through the feeding mechanism 4. The temperature and pH value of the reaction are respectively monitored by the second temperature sensor 7 and the pH sensor 8. The fermentation temperature is accurately adjusted by the temperature regulating mechanism 2. At the same time, the raw materials inside the fermentation tank body 12 are stirred by the mixing mechanism 3 to ensure uniform and sufficient fermentation of the raw materials. The oxygen concentration inside the water bath 11 is monitored and adjusted by the inert gas introduction mechanism 5. The feeding mechanism 4 and the inert gas introduction mechanism 5 respectively realize the raw material input and oxygen concentration control. The temperature regulating mechanism 2 uses the water bath 11, the heating component 210 and the No. 2 temperature sensor 7 to accurately control the temperature in the fermentation tank body 12 to maintain it at the set value; the mixing mechanism 3 fully stirs the raw materials in the fermentation tank body 12 to ensure uniform fermentation; the discharge pipe 13 and the No. 1 valve 14, the water discharge pipe 15 and the No. 2 valve 16 facilitate the discharge of materials and the replacement of water bath water; the pH sensor 8 in the fermentation tank body 12 monitors the reaction acidity and alkalinity in real time. The various parts work closely together, not only providing a stable environment for anaerobic fermentation, but also ensuring that the fermentation process is efficient and sufficient, effectively improving product quality and fermentation efficiency.

[0024] Example 3: Figure 2-Figure 9As shown, the temperature regulating mechanism 2 includes a No. 1 circulation pump 21, a cold water inlet pipe 28 and a heating component 210. The ends of the No. 1 circulation pump 21 are respectively equipped with a No. 1 connecting pipe 22 and a No. 2 connecting pipe 23. The No. 1 connecting pipe 22 is fixedly connected to the bottom of the water bath 11, and the No. 2 connecting pipe 23 is fixedly connected to the temperature conducting pipe 24. The temperature conducting pipe 24 is fixedly connected to the inner wall of the water bath 11, and the bottom of the temperature conducting pipe 24 is fixedly connected to a temperature conducting platform 26. A plurality of temperature conducting fins 27 are provided on the surface of the temperature conducting platform 26. A No. 3 connecting pipe 25 is fixedly connected to the side of the temperature conducting pipe 24. The No. 3 connecting pipe 25 is located outside the fermentation tank body 12. The cold water inlet pipe 28 is fixedly connected to the water bath 11, and a No. 3 valve 29 is installed on the surface of the cold water inlet pipe 28. The cold water inlet pipe 28 is externally connected to a cold water supply device. The heating component 210 is fixedly connected to the water bath 11, and a No. 1 temperature sensor 6 is fixedly connected to the surface of the water bath 11.

[0025] In this embodiment, when the pH value of the fermentation is too high or too low, an appropriate amount of acidifier or reducing agent is added to the fermentation tank body 12 through the feeding mechanism 4. When the temperature inside the fermentation tank body 12 is too high, cold water is injected into the water bath 11 through the cold water inlet pipe 28 to reduce the stability of the fermentation. When the fermentation temperature is too low, the water inside the water bath 11 is heated by the heating component 210 to increase the fermentation temperature. The temperature control method of the water bath improves the accuracy of temperature control and the stability of temperature regulation, thereby avoiding sudden temperature increases or decreases, which may cause the bacterial activity in the raw materials close to the inner wall of the fermentation tank body 12 to decrease or even die. At the same time, the water inside the water bath tank 11 is pumped through the No. 1 connecting pipe 22 through the No. 2 connecting pipe 23 and the temperature conducting pipe 24 by the No. 1 circulating pump 21, and finally falls back into the water bath tank 11 through the No. 3 connecting pipe 25. In this process, the water inside the water bath tank 11 can be mixed, so as to avoid the temperature of some areas being too low when cold water is passed in, and to avoid the water temperature of some areas being too high when the water is heated, thereby ensuring the stability of the water temperature of the water bath; When water passes through the temperature conducting pipe 24, the temperature of the water will be transferred to the temperature conducting platform 26 and the temperature conducting fin plate 27. When the raw materials return through the through hole at the bottom of the water wheel 37, they will first pass through the temperature conducting platform 26 and the temperature conducting fin plate 27, which can quickly cool down or heat up the raw materials, so that the raw materials can quickly reach the appropriate fermentation temperature.

[0026] A water circulation loop is formed by the No. 1 circulation pump 21, the No. 1 connecting pipe 22, the No. 2 connecting pipe 23, the temperature conducting pipe 24 and the No. 3 connecting pipe 25, which continuously stirs the water in the water bath 11 to avoid excessive local temperature differences caused by cold water injection or heating, ensuring uniform and stable water temperature; the cold water inlet pipe 28, the No. 3 valve 29 cooperate with the external cold water supply equipment to quickly inject cold water to cool down the fermentation tank body 12 when the temperature is too high, and the heating component 210 accurately heats up when the temperature is too low; the No. 1 temperature sensor 6 monitors the water bath temperature in real time to provide data support for regulation; the temperature conducting pipe 24, the temperature conducting platform 26 and the temperature conducting fin plate 27 form an efficient heat conduction structure, so that the raw materials flowing through it can quickly contact the appropriate temperature, reducing the risk of damage to the activity or death of the bacterial community near the inner wall of the fermentation tank body 12 due to sudden temperature changes. Through the dual mechanism of water bath temperature control and rapid heat exchange, the temperature regulation accuracy and stability are significantly improved, ensuring the efficient and stable fermentation process.

[0027] Example 4: Figure 2-Figure 9 As shown: the mixing mechanism 3 includes a No. 2 circulation pump 31, a discharge pipe 34, a connecting shaft 36, a water wheel 37 and a guide platform 39. The ends of the No. 2 circulation pump 31 are respectively installed with a No. 4 connecting pipe 32 and a No. 5 connecting pipe 33. The No. 4 connecting pipe 32 is fixedly connected to the fermentation tank body 12, and the No. 5 connecting pipe 33 is fixedly connected to the discharge pipe 34. The connecting shaft 36 is rotatably connected to the top of the water bath tank 11, and the connecting shaft 36 is fixedly connected to the water wheel 37. The discharge pipe 34 is located on the fermentation tank body 12. A plurality of discharge nozzles 35 located in the same horizontal plane and distributed in a ring are installed on the inner side of the discharge pipe 34. The discharge nozzles 35 are inclined at an angle toward the blade direction of the water wheel 37. A through hole is opened at the bottom of the water wheel 37, and the through hole is located above the temperature conduction platform 26. A stirring rod 38 is installed on the surface of the connecting shaft 36, and the stirring rod 38 is located inside the fermentation tank body 12. The guide platform 39 is fixedly connected to the connecting shaft 36, and a plurality of distribution plates 310 in a ring array are fixedly connected to the surface of the guide platform 39.

[0028] The feeding mechanism 4 includes a feeding pipe 41, which is fixedly connected to the water bath 11 and is located above the fermentation tank body 12. A No. 4 valve 42 is installed on the surface of the feeding pipe 41. The inert gas introduction mechanism 5 includes an air inlet pipe 51 and a dissolved oxygen sensor 53. The air inlet pipe 51 is fixedly connected to the water bath 11, and a No. 5 valve 52 is installed on the surface of the air inlet pipe 51. The dissolved oxygen sensor 53 is installed on the upper part of the water bath 11. The air inlet pipe 51 is externally connected to an inert gas supply device.

[0029] The raw materials in the fermentation tank body 12 are stirred by the stirring rod 38, and the raw materials fall back to the surface of the guide platform 39 through the through-hole provided at the bottom of the water wheel 37. The raw materials are evenly returned to the interior of the fermentation tank body 12 through the guide platform 39 and the dividing plate 310. During the whole stirring process, the raw materials are first mixed once when passing through the interior of the No. 2 circulation pump 31, and are mixed again when they are sprayed out through the discharge nozzle 35 and contact with the water wheel 37. Finally, they flow back to the interior of the fermentation tank body 12 and are stirred by the stirring rod 38. When the dissolved oxygen sensor 53 detects that the oxygen concentration in the water bath 11 is too high, inert gas is injected into the water bath 11 through the air inlet pipe 51 to replace the air inside the water bath 11.

[0030] Circulation pump No. 2 31 in mixing mechanism 3 pumps the raw materials from the fermenter body 12 to the discharge pipe 34 via connecting pipes No. 4 32 and No. 5 33. The high-speed jet ejected from the inclined discharge nozzle 35 impacts the blades of the water wheel 37, driving the connecting shaft 36 and stirring rod 38 to rotate. The raw materials achieve multi-stage mixing through the triple action of the pump body, the jet impact, and the stirring rod 38. The through-holes at the bottom of the water wheel 37 cooperate with the guide platform 39 and the material distribution plate 310 to evenly disperse the falling raw materials into the fermenter body 12, enhancing the mixing effect. Inert gas is introduced into mechanism 5. A dissolved oxygen sensor 53 monitors the oxygen concentration in the water bath 11 in real time. When the oxygen concentration exceeds the standard, inert gas is injected through the air inlet pipe 51 and valve No. 5 52 to rapidly replace the air and maintain an anaerobic environment. The two mechanisms work together to achieve efficient mixing without an additional power source through the principles of fluid mechanics. They also leverage intelligent sensing and gas displacement technology to precisely control fermentation conditions, significantly improving fermentation uniformity and product quality stability.

[0031] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A detoxifying and laxative enzyme based on pitaya and prebiotics, characterized in that: The raw materials include: 70-80% fermented pitaya pulp, 8-12% isomaltooligosaccharide, 0.05-0.1% composite bacteria, 0.1-0.2% vitamin C, and the balance is purified water; the pH value of the fermented pitaya pulp is 3.8-4.2, and the number of viable bacteria is ≥10 7 CFU / mL, anthocyanin content ≥20mg / 100mL; the purity of the oligosaccharide is ≥95%, and the proportion of 2-5 polymerization degree is ≥80%; the composite bacteria is composed of Lactobacillus acidophilus and Bifidobacterium BB-12.

2. The method for preparing a detoxifying and laxative enzyme based on pitaya and prebiotics according to claim 1, wherein: The following steps are involved: S1. Raw material pretreatment: The fermented pitaya pulp is processed through a double filter and a disc separator to obtain a clarified pulp liquid, which is temporarily stored under controlled temperature; oligomeric isomalto-oligosaccharide is prepared into a 50% concentration solution, which is filtered to remove impurities; S2. Segmented compounding and primary fermentation: After mixing the clarified raw pulp with a 5% by weight isomaltooligosaccharide solution, inoculate the pre-activated composite bacteria and ferment in an anaerobic fermentation tank at 37°C ± 0.5°C for 24 hours; S3. Secondary compounding and functional enhancement: After the fermentation is completed, filter and add 3% by weight of isomaltooligosaccharide solution and 0.1% of vitamin C, stir and mix, and then homogenize under high pressure of 120 MPa; S4. Sterilization and filling: After ultra-high pressure sterilization, the prepared liquid is quickly cooled and aseptically filled in a Class A clean area.

3. The method for preparing a detoxifying and laxative enzyme based on pitaya and prebiotics according to claim 2, wherein: During the primary fermentation process, the pH value and dissolved oxygen content were monitored every 2 hours by the DCS control system to maintain the pH value at 3.8-4.0 and the oxygen content at <0.5%.

4. The method for preparing a detoxifying and laxative enzyme based on pitaya and prebiotics according to claim 2, wherein: The ultra-high pressure sterilization condition is 600 MPa, maintaining the pressure for 3 minutes, and rapidly cooling to below 25° C. after sterilization.

5. The anaerobic fermentation tank according to claim 2, characterized in that: The invention comprises a fermentation mechanism (1), wherein the upper part of the fermentation mechanism (1) is fixedly connected to a feeding mechanism (4) and an inert gas introduction mechanism (5), and the side of the fermentation mechanism (1) is fixedly connected to a temperature regulating mechanism (2) and a mixing mechanism (3), wherein the fermentation mechanism (1) comprises a water bath (11), wherein the interior of the water bath (11) is fixedly connected to a fermentation tank body (12), wherein the mixing mechanism (3) is used to stir the raw materials inside the fermentation tank body (12), and wherein the bottom of the fermentation tank body (12) is fixedly connected to a discharge pipe ( 13), a No. 1 valve (14) is installed on the surface of the discharge pipe (13), a discharge pipe (15) is fixedly connected to the bottom of the water bath tank (11), and a No. 2 valve (16) is installed on the surface of the discharge pipe (15), the temperature regulating mechanism (2) is used to regulate the fermentation temperature inside the fermentation tank body (12), the inert gas introduction mechanism (5) is used to regulate the fermentation oxygen concentration inside the water bath tank (11), and a No. 2 temperature sensor (7) and a pH sensor (8) are respectively installed inside the fermentation tank body (12).

6. The anaerobic fermentation tank according to claim 5, characterized in that: The temperature regulating mechanism (2) comprises a No. 1 circulation pump (21), a cold water inlet pipe (28) and a heating assembly (210), wherein the ends of the No. 1 circulation pump (21) are respectively provided with a No. 1 connecting pipe (22) and a No. 2 connecting pipe (23), wherein the No. 1 connecting pipe (22) is fixedly connected to the bottom of the water bath tank (11), and the No. 2 connecting pipe (23) is fixedly connected to a temperature conducting pipe (24), wherein the temperature conducting pipe (24) is fixedly connected to the inner wall of the water bath tank (11), and the bottom of the temperature conducting pipe (24) is fixedly connected to a temperature conducting platform (26), wherein the temperature conducting platform (26) A plurality of temperature-conducting fins (27) are provided on the surface, a No. 3 connecting pipe (25) is fixedly connected to the side of the temperature-conducting pipe (24), and the No. 3 connecting pipe (25) is located outside the fermentation tank body (12). The cold water inlet pipe (28) is fixedly connected to the water bath tank (11), and a No. 3 valve (29) is installed on the surface of the cold water inlet pipe (28). The cold water inlet pipe (28) is externally connected to a cold water supply device, the heating component (210) is fixedly connected to the water bath tank (11), and a No. 1 temperature sensor (6) is fixedly connected to the surface of the water bath tank (11).

7. The anaerobic fermentation tank according to claim 6, characterized in that: The mixing mechanism (3) includes a No. 2 circulation pump (31), a discharge pipe (34), a connecting shaft (36), a water wheel (37) and a material guide platform (39). The ends of the No. 2 circulation pump (31) are respectively installed with a No. 4 connecting pipe (32) and a No. 5 connecting pipe (33). The No. 4 connecting pipe (32) is fixedly connected to the fermentation tank body (12), and the No. 5 connecting pipe (33) is fixedly connected to the discharge pipe (34). The connecting shaft (36) is rotatably connected to the top of the water bath tank (11), and the connecting shaft (36) is fixedly connected to the water wheel (37). The discharge pipe (34) is located at the fermentation tank body (12). 2) above, a plurality of discharge nozzles (35) located in the same horizontal plane and distributed in an annular manner are installed on the inner side of the discharge pipe (34), and the discharge nozzles (35) are inclined at an angle toward the direction of the blades of the water wheel (37). A through hole is opened at the bottom of the water wheel (37), and the through hole is located above the temperature conduction platform (26). A stirring rod (38) is installed on the surface of the connecting shaft (36), and the stirring rod (38) is located inside the fermentation tank body (12). The material guide platform (39) is fixedly connected to the connecting shaft (36), and a plurality of material distribution plates (310) in an annular array are fixedly connected to the surface of the material guide platform (39).

8. The anaerobic fermentation tank according to claim 7, characterized in that: The feeding mechanism (4) includes a feeding pipe (41), the feeding pipe (41) is fixedly connected to the water bath (11), and the feeding pipe (41) is located above the fermentation tank body (12), and a No. 4 valve (42) is installed on the surface of the feeding pipe (41). The inert gas introduction mechanism (5) includes an air intake pipe (51) and a dissolved oxygen sensor (53), the air intake pipe (51) is fixedly connected to the water bath (11), and a No. 5 valve (52) is installed on the surface of the air intake pipe (51), the dissolved oxygen sensor (53) is installed on the upper part of the water bath (11), and the air intake pipe (51) is externally connected to an inert gas supply device.

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