Full-automatic nucleic acid extractor

Abstract

The invention relates to the technical field of nucleic acid extraction instruments, in particular to a full-automatic nucleic acid extraction instrument, a portal frame module comprises a horizontally arranged portal frame base and a portal frame vertically arranged on the portal frame base, and the portal frame is slidably connected to the portal frame base; an oscillation module, a reagent strip bracket for placing a reagent strip and a reaction tube bracket for placing an eight-row tube are arranged at the upper part of the gantry base; the oscillation module is arranged on the oscillation module gantry base, and cell disruption of the sample is completed by the oscillation module through oscillation movement; one side of the gantry bracket is connected with the extraction module in a vertical sliding manner, and the other side is connected with the pipetting module in a vertical sliding manner; the pipetting module is used for transferring the sample subjected to cell wall breaking into each hole site in the reagent strip; the extraction module is used for extracting nucleic acid from the sample; and the pipetting module is also used for transferring the nucleic acid sample into the eight-row tube. The automation of the whole nucleic acid extraction process is realized.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid extraction instruments, in particular to a full-automatic nucleic acid extraction instrument. Background Art

[0002] Nucleic acids are carriers of genetic information, the most important bioinformatics molecules, and the primary research target of molecular biology. They also form the foundation for its extensive research and in-depth application. Nucleic acid extraction is the first step in molecular biology research and one of its most fundamental and important operations. Effective nucleic acid extraction from samples determines the success or failure of downstream experiments such as genetic testing and sequencing library construction.

[0003] In order to better realize the extraction of nucleic acids, more and more nucleic acid extraction instruments have emerged that use matching nucleic acid extraction reagents to automatically complete the extraction of sample nucleic acids. At this stage, they are widely used in disease control centers, clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbiology testing, food safety testing, animal husbandry and molecular biology research and other fields.

[0004] With the rapid development of the medical field, more and more nucleic acid-based biological experiments have emerged, and higher requirements have been placed on the total amount and purity of nucleic acid extraction. In order to obtain a larger total amount of nucleic acid, bacteria, fungi and other pathogens are often broken with a grinder or shaker before nucleic acid extraction. However, this pretreatment process introduces a certain degree of uncertainty, poor repeatability, and the operation process becomes cumbersome, with a low degree of automation, and high professional quality requirements for the experimental personnel.

[0005] Based on the shortcomings of the existing technology, there is an urgent need to develop a fully automatic nucleic acid extraction instrument to solve the above problems. Summary of the Invention

[0006] In order to solve the technical problem that “pathogens such as bacteria and fungi are first broken down using a grinder or shaker before nucleic acid extraction, but this pre-treatment process introduces certain uncertainties, poor repeatability, cumbersome operation, and low automation”, the present invention provides the following technical solutions: The present invention provides a fully automatic nucleic acid extraction instrument, characterized in that it comprises a gantry module, wherein the gantry module is equipped with an oscillation module, a pipetting module, and an extraction module; The gantry module includes a horizontally arranged gantry base and a gantry bracket vertically arranged on the gantry base, and the gantry bracket is slidably connected to the gantry base; The upper part of the gantry base is equipped with an oscillation module, a reagent strip holder for placing reagent strips, and a reaction tube holder for placing eight-tube strips; The oscillation module is arranged on the gantry base of the oscillation module and includes a sample tube rack for placing sample tubes. The sample tubes are filled with samples and glass beads. The oscillation module completes cell wall disruption of the samples through oscillation motion; One side of the gantry bracket slides up and down to connect to the extraction module, and the other side slides up and down to connect to the pipetting module; The pipetting module is used to transfer the sample after cell disruption to each well in the reagent strip through the tip of the pipette; The extraction module is used to move to each well of the reagent strip and extract nucleic acid from the sample by magnetic bead adsorption; The pipetting module is also used to move to the top of the well of the reagent strip where nucleic acid extraction is completed, and transfer the absorbed nucleic acid sample to the eight-tube strip through the tip of the gun.

[0007] In this technical solution, the vibration breaking, nucleic acid purification and pipetting functions are integrated into one, which further improves the working efficiency of the nucleic acid extraction instrument while ensuring the extraction effect, realizes the automation of the entire nucleic acid extraction process, and makes the nucleic acid extraction instrument have good application prospects.

[0008] Furthermore, the gantry module also includes a sample tube baffle, the oscillation module is arranged at one end of the gantry base, and the oscillation module is located below the sample tube baffle.

[0009] Furthermore, the oscillation module further includes an oscillation motor that is transmission-connected to the sample tube rack, and the sample tube rack performs oscillating motion under the drive of the oscillation motor.

[0010] Furthermore, the oscillation module includes an oscillation base, an oscillation side plate, an oscillation upper plate, and rubber columns. An oscillation motor is provided at the bottom of the oscillation upper plate, and a sample tube rack is provided at the top; the oscillation base is provided on the gantry base; the upper and lower positions of the two oscillation side plates are respectively connected to the oscillation base and the oscillation upper plate through rubber columns.

[0011] In this technical solution, the sample tube rack in the oscillation module performs left and right reciprocating motion driven by the oscillation motor. In the case of high-speed rotation, the shock absorption requirements of the mechanism will be increased. In the present invention, the upper and lower positions of the left and right oscillation side plates are respectively connected to the rubber columns, and the rubber columns are respectively connected to the oscillation base and the oscillation upper plate. When the oscillation upper plate moves back and forth at high speed, the rubber columns can weaken or even eliminate the impact of vibration on the oscillation base.

[0012] Furthermore, the vibration speed of the sample tube rack can reach 3500 rpm.

[0013] Furthermore, the oscillation module also includes a photoelectric switch and a photoelectric baffle, and the photoelectric baffle is arranged at one end of the sample tube rack; the photoelectric switch is arranged on the oscillation upper plate, and the photoelectric switch and the photoelectric baffle are located on the same side of the sample tube rack.

[0014] In this technical solution, when the sample tube rack in the oscillation module moves back and forth left and right under the drive of the oscillation motor, after the movement ends, the photoelectric baffle on the sample tube rack will search for the zero point, that is, the center position of the photoelectric switch. When the photoelectric baffle is at the center position of the photoelectric switch, the oscillation motor stops rotating and the sample tube rack stops at the set position.

[0015] Furthermore, the gantry module also includes a first drive motor and two first slide rails. The first drive motor is arranged at one end of the gantry base; the two first slide rails are respectively arranged on both sides of the gantry base, and the gantry bracket is slidably connected to the first slide rails; the first drive motor is connected to the first slide rails; the gantry bracket can move horizontally along the first slide rails.

[0016] Furthermore, the gantry module also includes a second drive motor and a second slide rail. The second drive motor is arranged on the top of the gantry bracket, and the second slide rail is vertically arranged on one side of the gantry bracket. The second drive motor is connected to the second slide rail, and the pipetting module is slidably connected to the second slide rail. The pipetting module can slide up and down along the second slide rail.

[0017] Furthermore, the gantry module also includes a third drive motor and a third slide rail. The third drive motor is arranged on the top of the gantry bracket, and the third slide rail is vertically arranged on the other side of the gantry bracket. The third drive motor is connected to the third slide rail, and the extraction module is slidably connected to the third slide rail. The extraction module can slide up and down along the third slide rail.

[0018] Furthermore, the extraction module also includes an extraction fixing plate, a magnetic rod fixing plate, and multiple magnetic rod sleeve fixing seats. The extraction fixing plate is arranged on the side of the gantry bracket away from the pipetting module, and the extraction fixing plate is slidably connected to the third slide rail; the upper part of the magnetic rod fixing plate is connected to the lower part of the extraction fixing plate; multiple magnetic rod sleeve fixing seats are provided at the bottom end of the magnetic rod fixing plate, and each magnetic rod sleeve fixing seat is matched and connected with a magnetic rod; when the magnetic rod fixing plate moves downward and moves to a certain position, the magnetic rod sleeve fixing seat will be inserted with the magnetic rod sleeve, and the magnetic rod moves to the bottom of the magnetic rod sleeve to adsorb magnetic beads to achieve nucleic acid extraction.

[0019] In this technical solution, when the third drive motor is working, the magnetic rod fixing plate in the extraction module moves up and down. It can be understood that when the third drive motor is working in the forward direction, the magnetic rod fixing plate will move downward, and the multiple magnetic rod sleeve fixing seats connected to the magnetic rod fixing plate will also move downward. After moving to a certain position, the magnetic rod sleeve fixing seat will be inserted into the magnetic rod sleeve, thereby achieving the purpose of adsorbing magnetic beads in the extraction process; During the extraction process, the magnetic rod can be moved to the bottom of the magnetic rod sleeve under the drive of the third driving motor to adsorb the magnetic beads to achieve the extraction of nucleic acid.

[0020] Furthermore, the extraction module also includes an extraction motor, and the top of the extraction fixing plate is connected to the extraction motor; after the extraction process is completed, the extraction motor drives the extraction fixing plate to move downward, removes the magnetic rod sleeve, and completes the shedding of the magnetic rod sleeve.

[0021] In this technical solution, after the extraction process is completed, the extraction motor starts to work forward, and the extraction fixing plate starts to move downward under the action of the driving force. The magnetic rod connected to the extraction fixing plate also moves downward until it moves to the set position and the magnetic rod cover can be removed, completing the shedding of the magnetic rod cover.

[0022] Furthermore, the number of the magnetic bars is 8.

[0023] Compared with the prior art, the present invention has the following beneficial effects: The present invention provides a fully automatic nucleic acid extraction instrument that integrates the functions of vibration wall breaking, nucleic acid purification and pipetting. While ensuring the extraction effect, it further improves the working efficiency of the nucleic acid extraction instrument, realizes the automation of the entire nucleic acid extraction process, and makes the nucleic acid extraction instrument have good application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a schematic diagram of the appearance of the entire instrument of the present invention from the axial side; Figure 2 This is a schematic diagram of the right front axle side of the entire machine of the present invention; Figure 3 This is a schematic diagram of the left rear axle side of the entire machine of the present invention; Figure 4 This is a schematic diagram of the gantry module structure in the present invention; Figure 5 This is a schematic diagram of the structure of the oscillation module in the present invention; Figure 6 Schematic diagram of the structure of the pipetting module in the present invention; Figure 7 This is a schematic diagram of the extraction module structure in the present invention.

[0025] Reference numerals: 1. Complete machine; 11. Gantry module; 12. Oscillation module; 13. Pipetting module; 14. Extraction module; 15. Electronic control module; 16. Housing module; 1101, gantry base; 1102, gantry bracket; 1103, first drive motor; 1104, first slide rail; 1105, second drive motor; 1106, second slide rail; 1107, third drive motor; 1108, third slide rail; 1109, sample tube baffle; 1110, reagent strip holder; 1111, reaction tube holder; 1201, oscillation base; 1202, oscillation side plate; 1203, oscillation upper plate; 1204, sample tube rack; 1205, oscillation motor; 1206, photoelectric switch; 1207, rubber column; 1208, photoelectric baffle; 1301, pipetting motor; 1302, pipetting fixing plate; 1303, pipetting slide; 1304, plunger fixing plate; 1305, plunger rod; 1306, pipetting chamber; 1307, tip head fixing plate; 1308, tip head fixing column; 1309, spring; 1310, shoulder screw; 1401, extraction fixing plate; 1402, extraction motor; 1403, magnetic rod fixing plate; 1404, magnetic rod sleeve fixing seat; 1405, magnetic rod; 1501, electronic control installation board; 1502, power module; 1503, main control board; 1504, motor drive board; 1601, housing base; 1602, rear housing; 1603, upper cover; 1604, indicator light; 1605, ground pin; 1606, power socket mounting plate; DETAILED DESCRIPTION The technical solution of the present invention will be clearly described below in conjunction with the accompanying drawings. Obviously, the described embodiments are not all embodiments of the present invention. All other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0026] It should be noted that the terms "center", "up", "down", "horizontal", "left", "right", "front", "back", "lateral", "longitudinal", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description. They do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0027] See also Figures 1 to 7 As shown, according to an embodiment of the present invention, a fully automatic nucleic acid extractor is provided, comprising a gantry module 11 , on which an oscillation module 12 , a pipetting module 13 , an extraction module 14 and an electronic control module 15 are mounted.

[0028] The gantry module 11 includes a gantry base 1101 arranged in the horizontal direction and a gantry bracket 1102 in the vertical direction. The gantry bracket 1102 is slidably connected to the gantry base 1101; a sample tube baffle 1109, a reagent strip bracket 1110 and a reaction tube bracket 1111 are arranged on the upper part of the gantry base 1101.

[0029] An oscillation module 12 is provided at one end of the gantry base 1101 , and the oscillation module 12 is located below the sample tube baffle 1109 .

[0030] A first drive motor 1103 is provided at one end of the gantry base 1101 , and two first slide rails 1104 are provided on both sides; the gantry bracket 1102 is slidably connected to the gantry base 1101 through the first slide rails 1104 ; the first drive motor 1103 is connected to the first slide rails 1104 .

[0031] A second driving motor 1105 is provided on the top of the gantry bracket 1102 , a second slide rail 1106 is provided on one side of the gantry bracket 1102 , the second driving motor 1105 is connected to the second slide rail 1106 , and the pipetting module 13 is connected to the second slide rail 1106 .

[0032] A third driving motor 1107 is provided on the top of the gantry support 1102 , and a third slide rail 1108 is provided on the other side of the gantry support 1102 . The third driving motor 1107 is connected to the third slide rail 1108 , and the extraction module 14 is connected to the third slide rail 1108 .

[0033] The oscillation module 12 includes a sample tube rack 1204 and an oscillation motor connected to the sample tube rack 1204; the sample tube rack 1204 performs oscillation motion under the drive of the oscillation motor 1205; one side of the gantry bracket 1102 slides in the up and down direction to connect to the extraction module 14, and the other side slides in the up and down direction to connect to the pipetting module 13.

[0034] The oscillation module 12 also includes an oscillation base 1201, an oscillation side plate 1202, an oscillation upper plate 1203, and a rubber column 1207. The oscillation upper plate 1203 is equipped with an oscillation motor 1205 at the bottom and a sample tube rack 1204 at the top; the upper and lower positions of the two oscillation side plates 1202 are respectively connected to the oscillation base 1201 and the oscillation upper plate 1203 through the rubber column 1207.

[0035] The oscillation module 12 further includes a photoelectric switch 1206 and a photoelectric baffle 1208 . The photoelectric baffle 1208 is disposed on one end of the sample tube rack 1204 . The photoelectric switch 1206 is located on the oscillation upper plate 1203 and is located on the same side of the sample tube rack 1204 as the photoelectric baffle 1208 .

[0036] The oscillation module 12 is connected to the gantry base 1101 in the gantry module 11. The sample tube rack 1204 in the oscillation module 12 performs left and right reciprocating motion driven by the oscillation motor 1205. The vibration speed can reach 3500 rpm or higher. After the motion is completed, the photoelectric baffle 1208 on the sample tube rack 1204 will find the zero point, that is, the center position of the photoelectric switch 1206. When the photoelectric baffle 1208 is located at the center position of the photoelectric switch 1206, the oscillation motor 1205 stops rotating and the sample tube rack 1204 stops at the set position. It can be understood that under high-speed rotation, the shock absorption requirements of the mechanism will be increased. In the present invention, the left and right oscillation side plates 1202 are respectively connected to the rubber columns 1208 at the upper and lower positions. The rubber columns 1208 are respectively connected to the oscillation base 1201 and the oscillation upper plate 1203. When the oscillation upper plate 1203 moves back and forth at high speed, the rubber columns 1208 weaken or even eliminate the impact of vibration on the oscillation base 1201.

[0037] The pipetting module 13 and the extraction module 14 are connected to the gantry bracket 1102, and the gantry bracket 1102 is connected to the first drive motor 1103 through two left and right first slide rails 1104. It can be understood that when the first drive motor 1103 is working, the gantry bracket 1102 will move back and forth, and the corresponding pipetting module 13 and the extraction module 14 will also move back and forth.

[0038] The pipetting module 13 includes a plunger fixing plate 1304, a plunger rod 1305, a pipetting motor 1301, and a tip head fixing column 1308. The pipetting motor 1301 is connected to the plunger fixing plate 1304, the plunger fixing plate 1304 is connected to the plunger rod 1305, and the plunger rod 1305 is connected to the tip head fixing column 1308. The pipetting motor 1301 can drive the plunger fixing plate 1304 to move up and down, driving the tip gun head connected to the tip head fixing column 1308 to suck in or expel gas or liquid.

[0039] The pipetting module 13 further includes a pipetting fixing plate 1302 and a pipetting cavity 1306 , and the pipetting fixing plate 1302 is connected to the second slide rail 1106 ; The pipetting fixed plate 1302 is equipped with a pipetting motor 1301 and a pipetting slide 1303. The bottom of the pipetting motor 1301 is connected to the plunger fixing plate 1304. The pipetting cavity 1306 is located below the plunger fixing plate 1304. The pipetting cavity 1306 is equipped with multiple plunger rods 1305 and multiple tip head fixing columns 1308 arranged at the bottom, the number of which is the same as the number of plunger rods 1305. The plunger fixing plate is connected to the pipetting slide 1303.

[0040] The pipetting module 13 also includes a tip head withdrawal fixing plate 1307, a shoulder screw 1310 and a spring 1309, and the shoulder screw 1310 is arranged at both ends of the top of the pipetting cavity 1306 through the spring 1309; the tip head withdrawal fixing plate 1307 is detachably connected to the bottom of the pipetting cavity 1306, and a plurality of tip head fixing columns 1308 are arranged on the tip head withdrawal fixing plate 1307.

[0041] The pipetting module 13 is connected to the second slide rail 1106 on the gantry bracket 1102 and is also connected to the second drive motor 1105. When the second drive motor 1105 is working, the pipetting module 13 moves up and down as a whole. At the same time, the plunger fixing plate 1304 on the pipetting module 13 is connected to the pipetting slide rail 1303 and the pipetting motor 1301. When the pipetting motor 1301 is working, the plunger fixing plate 1304 moves up and down. It can be understood that when one or more tip head fixing columns are equipped with tip gun heads, if the plunger fixing plate 1304 moves upward at this time, When the tip is moved, the tip gun head will suck in gas or liquid, otherwise the tip gun head will expel gas or liquid; when the plunger fixing plate 1304 continues to move downward and touches the shoulder screw 1310, the shoulder screw 1310 will move downward with the retracting tip head fixing plate 1307. When it moves down to a certain position, the retracting tip head fixing plate 1307 will retract the tip gun head, thereby achieving the purpose of retracting the gun head. After the pipetting motor 1301 is reset, the plunger fixing plate 1304 moves up to zero position, and the retracting tip head fixing plate 1307 returns to zero position under the reverse force of the spring 1309.

[0042] The extraction module 14 includes a magnetic rod fixing plate 1403 and multiple magnetic rod sleeve fixing seats 1404 connected to it. The magnetic rod sleeve fixing seats 1404 are matched with magnetic rods 1405. The magnetic rod fixing plate 1403 moves downward. After moving to a certain position, the magnetic rod sleeve fixing seat 1404 will be inserted with a magnetic rod sleeve. The magnetic rod 1405 moves to the bottom of the magnetic rod sleeve to adsorb magnetic beads to achieve nucleic acid extraction.

[0043] The extraction module 14 further includes an extraction fixing plate 1401 and an extraction motor 1402 ; the extraction fixing plate 1401 is disposed on a side of the gantry support 1102 away from the pipetting module 13 , and the extraction fixing plate 1401 is slidably connected to the third slide rail 1108 ; The top of the extraction fixing plate 1401 is connected to the extraction motor 1402 , and the top of the magnetic bar fixing plate 1403 is connected to the bottom of the extraction fixing plate 1401 .

[0044] In this embodiment, the number of the magnetic rod sleeve fixing seats is 8.

[0045] The extraction module 14 is connected to the third slide rail 1108 on the gantry support 1102 and is also connected to the third drive motor 1107. When the third drive motor 1107 is working, the magnetic bar fixing plate 1403 in the extraction module 14 moves up and down. It is understandable that when the third drive motor 1107 is working in the forward direction, the magnetic bar fixing plate 1403 will move downward, and the eight magnetic bar sleeve fixing seats 1404 connected to the magnetic bar fixing plate 1403 will also move downward. After moving to a certain position, the magnetic bar sleeve fixing seats 1404 will be inserted with magnetic bar sleeves, thereby achieving the purpose of adsorbing magnetic beads in the extraction process. During the extraction process, the magnetic bar 1405 can be moved to the bottom of the magnetic bar sleeve under the drive of the third drive motor 1107 to adsorb magnetic beads and achieve nucleic acid extraction. After the extraction process is completed, the extraction motor 1402 starts to work forward, and the extraction fixing plate 1401 starts to move downward under the action of the driving force. The eight magnetic bars 1405 connected to the extraction fixing plate 1401 also move downward until they move to the set position and the magnetic bar cover can be removed, completing the shedding of the magnetic bar cover.

[0046] The electric control module 15 includes an electric control installation board 1501 , a power module 1502 , a main control board 1503 and a motor drive board 1504 .

[0047] The electronic control module 15 is connected to the gantry bracket 1102 through the electronic control installation board 1501. The power module 1502 of the electronic control installation board 1501 is responsible for the power supply of the whole machine. The main control board 1503 of the electronic control installation board 1501 is responsible for all command transmission and control of the whole machine. The motor drive board 1504 of the electronic control installation board 1501 is responsible for the control of the oscillation motor 1205.

[0048] The fully automatic nucleic acid extractor further includes the housing module 16 , which includes a housing base 1601 , a rear shell 1602 , an upper cover 1603 , a foot 1605 , an indicator light 1604 and a power socket mounting plate 1606 .

[0049] In order to further understand the embodiments of the present invention, the following further describes the operation process of the present invention: Place the sample tube containing the sample and glass beads into the sample tube rack 1204 , place the reagent strip with pre-packaged reagents and consumables into the reagent strip holder 1110 , and place the eight-tube strip into the reaction tube holder 1111 .

[0050] First, the oscillation motor 1205 starts to rotate at high speed, causing the sample tube rack 1204 to move the sample tube in a high-speed reciprocating motion. After the vibration time is set, the oscillation motor 1205 stops working, and the sample tube rack 1204 moves to the zero position, completing the cell wall disruption process of the sample in the sample tube. Then, the gantry support 1102 starts to move under the action of the first drive motor 1103, driving the pipetting module 13 to move to the top of the tip in the reagent strip. The second drive motor 1105 starts to rotate forward, and the pipetting module 13 moves downward as a whole until the tip fixing column 1308 is inserted into the tip. The second drive motor 1105 starts to rotate in the reverse direction, and the pipetting module 13 returns to the zero position and then moves to the top of the sample tube. The tip of the gun moves to the inside of the sample tube under the action of the second drive motor 1105, the pipetting motor 1301 starts to rotate in the opposite direction, the plunger rod 1305 moves upward, and the tip of the gun starts to aspirate liquid. After the set amount of liquid aspirated is completed, the tip of the gun moves upward to the original position, and the pipetting module 13 as a whole moves backward to above the reagent strip hole under the action of the first drive motor 1103; The tip of the gun moves to the inside of the reagent strip hole under the action of the second drive motor 1105, the pipetting motor 1301 starts to rotate forward, the plunger rod 1305 moves downward, and the tip of the gun starts to discharge liquid. After the discharge is completed, the tip of the gun moves upward to the original position; The pipetting module 13 as a whole moves backward to just above the tip of the reagent strip under the action of the first drive motor 1103, and the tip of the reagent strip is moved to the inside of the reagent strip hole under the action of the second drive motor 1105. The pipetting motor 1301 starts to rotate forward, and the plunger rod 1305 moves downward, touches the shoulder screw 1310, and continues to move downward until the tip head fixing plate 1307 retracts the tip of the reagent strip, and then the pipetting motor 1301 returns to zero position.

[0051] Then, the extraction module 14 begins to move to the top of the magnetic rod sleeve hole in the reagent strip under the action of the first drive motor 1103, and the magnetic rod sleeve fixing seat 1404 moves downward under the drive of the extraction motor 1402 until the magnetic rod sleeve is inserted, and then the magnetic rod sleeve fixing seat 1404 moves up to the zero position. The extraction module 14 is driven by the first drive motor 1103 to move to each hole position of the reagent strip in turn. Through the high-frequency forward and reverse rotation of the extraction motor 1402, the magnetic rod sleeve can be driven to move up and down at high speed to achieve the effect of oscillation and mixing. In addition, the magnetic rod 1405 can be moved to the bottom of the magnetic rod sleeve under the drive of the third drive motor 1107 to achieve the purpose of attracting magnetic beads.

[0052] After completing the extraction process, the extraction module 14 returns to the top of the magnetic rod sleeve hole in the reagent strip under the action of the first drive motor 1103, and the magnetic rod 1405 moves downward under the drive of the third drive motor 1107 until the magnetic rod sleeve is removed. Then the pipetting module 13 moves to the top of the tip gun head in the reagent strip, inserts the second tip head, and moves back to the zero position, and continues to move to the top of the hole position where the nucleic acid is extracted in the reagent strip, and sucks the set volume of nucleic acid downward and transfers it to the eight-tube strip. At this point, the instrument completes the entire process.

[0053] The above technical features constitute the best embodiment of the present invention, which has strong adaptability and best implementation effect. Non-essential technical features can be added or removed according to actual needs to meet the needs of different situations.

[0054] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A fully automatic nucleic acid extraction instrument, characterized in that: It includes a gantry module, on which an oscillation module, a pipetting module, and an extraction module are installed; The gantry module includes a horizontally arranged gantry base and a gantry bracket vertically arranged on the gantry base, and the gantry bracket is slidably connected to the gantry base; The upper part of the gantry base is equipped with an oscillation module, a reagent strip holder for placing reagent strips, and a reaction tube holder for placing eight-tube strips; The oscillation module is arranged on the gantry base of the oscillation module and includes a sample tube rack for placing sample tubes. The sample tubes are filled with samples and glass beads. The oscillation module completes cell wall disruption of the samples through oscillation motion; One side of the gantry bracket slides up and down to connect to the extraction module, and the other side slides up and down to connect to the pipetting module; The pipetting module is used to transfer the sample after cell disruption to each well in the reagent strip through the tip of the pipette; The extraction module is used to move to each well of the reagent strip and extract nucleic acid from the sample by magnetic bead adsorption; The pipetting module is also used to move to the top of the well of the reagent strip where nucleic acid extraction is completed, and transfer the absorbed nucleic acid sample to the eight-tube strip through the tip of the gun.

2. The fully automatic nucleic acid extractor according to claim 1, characterized in that The gantry module also includes a sample tube baffle. The oscillation module is arranged at one end of the gantry base, and the oscillation module is located below the sample tube baffle.

3. The fully automatic nucleic acid extractor according to claim 2, characterized in that: The oscillation module further includes an oscillation motor that is transmission-connected to the sample tube rack, and the sample tube rack performs oscillating motion under the drive of the oscillation motor.

4. The fully automatic nucleic acid extractor according to claim 3, characterized in that: The oscillation module also includes an oscillation base, an oscillation side plate, an oscillation upper plate, and rubber columns. An oscillation motor is provided at the bottom of the oscillation upper plate, and a sample tube rack is provided at the top; the oscillation base is provided on the gantry base; the upper and lower positions of the two oscillation side plates are respectively connected to the oscillation base and the oscillation upper plate through rubber columns.

5. The fully automatic nucleic acid extractor according to claim 4, characterized in that: The sample tube rack can vibrate at a speed of up to 3500 rpm.

6. The fully automatic nucleic acid extractor according to claim 4, characterized in that: The oscillation module also includes a photoelectric switch and a photoelectric baffle, wherein the photoelectric baffle is arranged at one end of the sample tube rack; the photoelectric switch is arranged on the oscillation upper plate, and the photoelectric switch and the photoelectric baffle are located on the same side of the sample tube rack.

7. The fully automatic nucleic acid extractor according to any one of claims 1 to 6, characterized in that: The gantry module also includes a first drive motor and two first slide rails. The first drive motor is arranged at one end of the gantry base; the two first slide rails are respectively arranged on both sides of the gantry base, and the gantry bracket is slidably connected to the first slide rails; the first drive motor is connected to the first slide rails; the gantry bracket can move horizontally along the first slide rails.

8. The fully automatic nucleic acid extractor according to claim 7, characterized in that: The gantry module also includes a second drive motor and a second slide rail. The second drive motor is arranged on the top of the gantry bracket, and the second slide rail is vertically arranged on one side of the gantry bracket. The second drive motor is connected to the second slide rail, and the pipetting module is slidably connected to the second slide rail. The pipetting module can slide up and down along the second slide rail.

9. The fully automatic nucleic acid extractor according to claim 8, characterized in that: The gantry module also includes a third drive motor and a third slide rail. The third drive motor is arranged on the top of the gantry bracket, and the third slide rail is vertically arranged on the other side of the gantry bracket. The third drive motor is connected to the third slide rail, and the extraction module is slidably connected to the third slide rail. The extraction module can slide up and down along the third slide rail.

10. The fully automatic nucleic acid extractor according to claim 9, characterized in that: The extraction module also includes an extraction fixing plate, a magnetic rod fixing plate, and multiple magnetic rod sleeve fixing seats. The extraction fixing plate is arranged on the side of the gantry bracket away from the pipetting module, and the extraction fixing plate is slidably connected to the third slide rail; the upper part of the magnetic rod fixing plate is connected to the lower part of the extraction fixing plate; multiple magnetic rod sleeve fixing seats are provided at the bottom end of the magnetic rod fixing plate, and each magnetic rod sleeve fixing seat is matched and connected with a magnetic rod; when the magnetic rod fixing plate moves downward and moves to a certain position, the magnetic rod sleeve fixing seat will be inserted into the magnetic rod sleeve, and the magnetic rod will move to the bottom of the magnetic rod sleeve to adsorb magnetic beads to achieve nucleic acid extraction.

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