Method for shortening dictyophora rubrovolvata stick culture period

Abstract

The invention belongs to the field of edible fungus culture, and particularly relates to a method for shortening the culture period of dictyophora rubrovolvata sticks. In the prior art, the culture period of dictyophora rubrovolvata sticks is too long, the pollution rate is too high, and the economic benefit of dictyophora rubrovolvata planting is influenced, the method provided by the invention can shorten the culture period of the dictyophora rubrovolvata sticks from 120 days to 60 days, the culture period of the dictyophora rubrovolvata sticks is greatly shortened, and meanwhile, the economic benefit of dictyophora rubrovolvata planting According to the method provided by the invention, the pollution rate of the dictyophora rubrovolvata sticks can be controlled to be 1-2%, and the pollution rate of the dictyophora rubrovolvata sticks is reduced.

Description

[0001] The invention belongs to the field of edible fungus cultivation, and particularly relates to a method for shortening the cultivation period of red-stalked bamboo fungus sticks. Background Art

[0002] Red-stemmed Dictyophora, a dual-purpose edible and medicinal fungus from the Phalloidaceae family and the genus Dictyophora, is delicious and tender. Fresh Dictyophora can be cooked in a variety of ways, including roasting, stir-frying, braising, grilling, stewing, and hot pot. It is highly sought after by consumers, commands a high market price, and offers good economic returns. With the maturity and widespread adoption of artificial cultivation techniques for Red-stemmed Dictyophora, its cultivation has gradually expanded, forming a substantial industry, driving economic development in the growing regions and increasing farmers' incomes.

[0003] The growth process of Dictyophora rubra is a complex one, from hyphae germination to fruiting body maturity. It can be divided into the mycelial growth stage and the fruiting body development stage. The mycelial growth stage is a critical period for Dictyophora rubra to accumulate nutrients, primarily through the growth and expansion of hyphae within the culture medium or substrate, forming a reserve energy for subsequent fruiting bodies. The mycelial growth stage can be broadly divided into three phases: hyphae germination, hyphae expansion, and hyphae maturation. After the mycelial maturity stage, the finished product is typically mature mushroom sticks, which are firm, free of yellow water, and do not soften when pinched, marking the entry into the fruiting body stage.

[0004] The cultivation cycle of red-stemmed bamboo fungus is affected by the cultivation environment, culture medium formula and management technology. At present, the domestic factory-produced red-stemmed bamboo fungus sticks use ordinary polyethylene plastic bags, and are inoculated with solid or liquid fungus. The cultivation cycle is about 120 days, and the fungus sticks are full of fungi. Under normal circumstances, the contamination rate of the finished product is between 5 and 10%.

[0005] Under the existing technology, the planting cycle of red-stemmed bamboo fungus sticks is long and disease prevention and control is difficult, which affects the economic benefits of red-stemmed bamboo fungus planting. Summary of the Invention

[0006] In order to solve the problems of long culture period and high contamination rate of red stem bamboo fungus sticks in the prior art, the present invention provides a method for shortening the culture period of red stem bamboo fungus sticks.

[0007] This is achieved specifically through the following technical solutions: A method for shortening the culture period of red-stalked bamboo fungus sticks, including making the sticks and regulating the growth of the sticks: The method for making a mushroom stick comprises the following steps: 1) Preparation of red stem fungus culture medium Calculated by mass, 60 to 80% of sawdust, 15 to 21% of wheat bran, 2 to 8% of cornmeal, 0.5 to 1% of sugar, 0.3 to 1% of lime, and 0.5 to 1.2% of gypsum are added into a mixing machine and stirred uniformly to obtain a mixture 1; adding water to the mixture in a ratio of 1:0.55 to 1:0.65, and adjusting the pH with lime to a pH range of 7.5 to 8.2 to obtain a mixture 2; Add 2 to 4% humus soil, 0.7 to 1.5% humic acid, and 0.7 to 1.5% glucose to the mixture according to mass percentage to obtain a mixture 3, which is the red-tubed bamboo fungus culture medium; 2) Making of filling bags When making the red-stemmed bamboo fungus compost, the size of the packing bag is 16 cm to 19 cm in width and 30 to 35 cm in length, and the punching range is 15*2.3 cm and is located in the middle of the packing belt; the breathable patch is made of high-density polypropylene fiber non-woven fabric with a mass of 80 to 150 g / m2, and the length and width of the breathable patch are 2 to 4 cm; the total weight of the red-stemmed bamboo fungus compost after bagging is 1000 to 1500 g.

[0008] 3) Preparation of mushroom bags Weigh 1 to 1.5 kg of Dictyophora rubra culture medium into the prepared packing bag in step 2), seal it, and use it for subsequent culture. 4) Inoculation of bacteria Use the liquid spawn of Dictyophora rubra fermented in the fermentation tank and inoculate each mushroom stick with 30 to 50 ml, inoculating at two points. After inoculation, transfer it to the culture room and culture it for 60 days to obtain the prepared mushroom sticks.

[0009] The mushroom stick growth control method comprises the following steps: 1) Germination period regulation Two days before storage, adjust the temperature in the warehouse to 30℃ and the air humidity to 40% and maintain it for two days.

[0010] On the day of storage and the next day, adjust the temperature in the warehouse to 28℃ and the air humidity to 40%.

[0011] On the third day after storage, the temperature in the warehouse was adjusted to 26°C and the air humidity was not controlled.

[0012] On the fourth day after storage, the temperature in the warehouse was adjusted to 24°C and the air humidity was not controlled.

[0013] On the fifth day after storage, the temperature in the warehouse was adjusted to 22°C and the air humidity was not controlled.

[0014] Subsequently, until the fever period, the temperature in the warehouse was maintained at 20°C and the air humidity was not controlled.

[0015] 2) Fever period control On the 51st to 52nd day after storage, the temperature in the warehouse was adjusted to 20°C, the carbon dioxide concentration was 4500PPM, and the internal circulation in the warehouse was set to start the fan for 15 to 20 minutes and then shut down for 60 minutes, repeating this cycle.

[0016] On the 53rd to 54th day after storage, the temperature in the warehouse was adjusted to 19°C, the carbon dioxide concentration was 4000PPM, and the internal circulation in the warehouse was set to start for 20 to 25 minutes every hour and then shut down for 60 minutes, repeating this cycle.

[0017] On the 55th to 56th day after storage, the temperature in the warehouse was adjusted to 18°C, the carbon dioxide concentration was 3500 PPM, and the internal circulation system in the warehouse was set to start for 20 to 25 minutes, then shut down for 60 minutes, and so on.

[0018] On the 57th to 58th day after storage, the temperature in the warehouse was adjusted to 17°C and the carbon dioxide concentration was 3000PPM. The internal circulation setting in the warehouse was turned on for 25 to 30 minutes and then turned off for 60 minutes, and this cycle was repeated.

[0019] On the 59th to 60th day after storage, adjust the temperature in the warehouse to 16°C and the carbon dioxide concentration to 2500PPM. The internal circulation setting in the warehouse is turned on for 25 to 30 minutes and then turned off for 60 minutes, and this cycle repeats.

[0020] Mature mushroom sticks can be harvested on the 60th day after storage.

[0021] Beneficial effects The present invention provides a method for shortening the culture period of red-suspension bamboo fungus sticks, which has the following effects: 1. The method provided by the present invention can shorten the culture period of Dictyophora rubra from 120 days to 60 days, significantly shortening the culture period; 2. The method provided by the present invention can control the contamination rate of red-suspension bamboo fungus sticks to between 1% and 2%, thereby reducing the contamination rate of the mushroom sticks. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 The bacterial sticks after inoculation of the bacterial strain in Example 1; Figure 2 Example 1: After 40 days of growth, the mushroom sticks were stored; Figure 3 Example 1: After 50 days of growth, the mushroom sticks were stored; Figure 4 Example 1: Bacteria sticks were grown in the warehouse for 60 days. DETAILED DESCRIPTION

[0023] Example 1 1) Preparation of red stem fungus culture medium Calculated by mass, 75% sawdust, 18% wheat bran, 5% cornmeal, 0.8% sugar, 0.5% lime, and 0.7% gypsum were added into a mixing machine and stirred evenly to obtain a mixture 1; Water was added to Mix 1 in a ratio of 1:1:0.6, and the pH was adjusted to 7.8 with lime powder to obtain Mix 2.

[0024] 3% humus soil, 1% humic acid and 1% glucose were added to the mixture according to mass percentage to obtain mixture 3, which is the culture medium for Dictyophora rubra.

[0025] 2) Making of filling bags When making the red-stemmed bamboo fungus compost, the size of the packing bag is 16 cm wide by 33 cm long, and the punching range is 15*2.3 cm located in the middle of the packing belt; the breathable patch is made of high-density polypropylene fiber non-woven fabric with a mass of 80 g / m2, and the length and width of the breathable patch are 2 cm; the total weight of the red-stemmed bamboo fungus compost after bagging is 1000 to 1500 g.

[0026] 3) Preparation of mushroom bags Weigh 1 kg of Dictyophora rubra culture medium and place it into the packing bag prepared in step 2), seal it and use it for subsequent culture. 4) Inoculation of bacteria The liquid spawn of Dictyophora rubra fermented in the fermentation tank was inoculated with an inoculation gun, with 30 ml inoculated into each bacterial stick at two points, one above the other. After inoculation, the mixture was transferred to the culture room and cultured for 60 days to obtain the prepared bacterial sticks.

[0027] After inoculating the liquid fungus of Dictyophora rubra, germination was regulated according to the conditions in Table 1 below.

[0028] Table 1 Control settings for the germination period of red bamboo fungus packages

[0029] After inoculation of the liquid spawn of Dictyophora rubra, the fever period was regulated according to the conditions in Table 2 below.

[0030] Table 2 Control settings for the fever period of red bamboo fungus

[0031] 100,000 mushroom sticks were produced from July 9 to 11, 2024. This batch of mushroom sticks were shipped from September 7 to 9, 2024. The statistics of the mushroom sticks are as follows: the mushroom stick fullness rate is 99.5%, and the contamination rate is 0.5%.

[0032] During the whole culture process, there is a 1 to 2 cm gap between the mushroom sticks. Figure 1 shown.

[0033] Figures 2 to 4 The growth status of the mushroom sticks after 40 days, 50 days and 60 days of growth. Figures 2 to 4 It can be seen that the white mycelium inside the mushroom stick gradually spreads downward until it completely covers the culture medium, with no obvious uncolonized blank areas. The mycelium is initially pure white, gradually turning milky white or light yellow as it matures. At 60 days, when pressed by hand, mature mushroom sticks are characterized by a firm, elastic texture that is resistant to breakage, with no green, black, or red spots on the surface or inside. There is no musty or sour odor, but a faint mushroom fragrance or wood chip odor, and white, rice-sized bumps appear on the surface of the mushroom stick.

[0034] The method provided by the present invention can shorten the culture period of red-stemmed bamboo fungus sticks from 120 days to 60 days, significantly shortening the culture period of the sticks. At the same time, the method provided by the present invention can control the contamination rate of red-stemmed bamboo fungus sticks to between 1% and 2%, reducing the contamination rate of the sticks.

[0035] Example 2 1) Preparation of red stem fungus culture medium Calculated by mass, 71% sawdust, 23% wheat bran, 4% cornmeal, 0.8% sugar, 0.5% lime, and 0.7% gypsum were added into a mixing machine and stirred uniformly to obtain a mixture 1; Water was added to Mix 1 in a ratio of 1:1:0.6, and the pH was adjusted to 7.8 with lime powder to obtain Mix 2.

[0036] 3% humus soil, 1% humic acid and 1% glucose were added to the mixture according to mass percentage to obtain mixture 3, which is the culture medium for Dictyophora rubra.

[0037] 2) Making of filling bags When making the red-stemmed bamboo fungus compost, the size of the packing bag is 17 cm wide by 33 cm long, and the punching range is 15*2.3 cm and is located in the middle of the packing belt; the breathable patch is made of high-density polypropylene fiber non-woven fabric with a mass of 100 g / m2 and a length and width of 2.5 cm; the total weight of the red-stemmed bamboo fungus compost after bagging is 1000 to 1500 g.

[0038] 3) Preparation of mushroom bags Weigh 1.2 kg of Dictyophora rubra culture medium and place it into the packing bag prepared in step 2), seal it and use it for subsequent culture. 4) Inoculation of bacteria The liquid spawn of Dictyophora rubra fermented in the fermentation tank was inoculated with an inoculation gun, with 40 ml inoculated into each bacterial stick at two points, one above the other. After inoculation, the mixture was transferred to the culture room and cultured for 60 days to obtain the prepared bacterial sticks.

[0039] After inoculation of the liquid fungus of Dictyophora rubra, germination was regulated according to the conditions in Table 3 below.

[0040] Table 3 Control settings for the germination period of red bamboo fungus packages

[0041] After inoculation of the liquid spawn of Dictyophora rubra, the fever period was regulated according to the conditions in Table 4 below.

[0042] Table 4 Control settings for the fever period of red bamboo fungus

[0043] 50,000 mushroom sticks were produced from March 16 to 17, 2024. This batch of mushroom sticks was shipped from May 15 to 16, 2024. The statistics of the mushroom sticks are as follows: the mushroom stick fullness rate is 99.6%, and the contamination rate is 0.7%.

[0044] The initial mycelium is pure white, and gradually turns into milky white or light yellow after maturity. After 60 days, press the mushroom stick with your hand. The characteristics of the mature mushroom stick are firm and elastic, not easy to break, and there are no green, black, red and other fungal spots on the surface and inside of the mushroom stick. There is no moldy or sour smell, but a faint mushroom fragrance or sawdust smell, and white, rice-sized bumps appear on the surface of the mushroom stick.

[0045] The method provided by the present invention can shorten the culture period of red-stemmed bamboo fungus sticks from 120 days to 60 days, significantly shortening the culture period of the sticks. At the same time, the method provided by the present invention can control the contamination rate of red-stemmed bamboo fungus sticks to between 1% and 2%, reducing the contamination rate of the sticks.

[0046] Example 3 1) Preparation of red stem fungus culture medium Calculated by mass, 73% sawdust, 22% wheat bran, 3% cornmeal, 0.8% sugar, 0.5% lime, and 0.7% gypsum were added into a mixing machine and stirred evenly to obtain a mixture 1; Water was added to the mixture 1 in a ratio of 1:1:0.6, and the pH was adjusted to 7.8 with hydrochloric acid and sodium hydroxide to obtain a mixture 2.

[0047] 3% humus soil, 1% humic acid and 1% glucose were added to the mixture according to mass percentage to obtain mixture 3, which is the culture medium for Dictyophora rubra.

[0048] 2) Making of filling bags When making the red-stemmed bamboo fungus compost, the size of the packing bag is 18 cm wide by 33 cm long, and the punching range is 15*2.3 cm and is located in the middle of the packing belt; the breathable patch is made of high-density polypropylene fiber non-woven fabric with a mass of 120 g / m2, and the length and width of the breathable patch are 2 cm; the total weight of the red-stemmed bamboo fungus compost after bagging is 1000 to 1500 g.

[0049] 3) Preparation of mushroom bags Weigh 1.3 kg of Dictyophora rubra culture medium and place it into the packing bag prepared in step 2), seal it and use it for subsequent culture. 4) Inoculation of bacteria The liquid spawn of Dictyophora rubra fermented in the fermentation tank was inoculated with an inoculation gun, with 50 ml inoculated into each bacterial stick at two points, one above the other. After inoculation, the mixture was transferred to the culture room and cultured for 60 days to obtain the prepared bacterial sticks.

[0050] After inoculation of the liquid fungus of Dictyophora rubra, germination was regulated according to the conditions in Table 5 below.

[0051] Table 5 Control settings for the germination period of red bamboo fungus packages

[0052] After inoculating the liquid fungus of Dictyophora rubra, the fever period was regulated according to the conditions in Table 6 below. Table 6 Control settings for the fever period of red bamboo fungus

[0053] 100,000 mushroom sticks were produced from June 5 to 6, 2024. This batch of mushroom sticks were harvested from August 4 to 5, 2024. The statistics of the mushroom sticks are as follows: the mushroom stick fullness rate is 99.4%, and the contamination rate is 0.6%.

[0054] The initial mycelium is pure white, and gradually turns into milky white or light yellow after maturity. After 60 days, press the mushroom stick with your hand. The characteristics of the mature mushroom stick are firm and elastic, not easy to break, and there are no green, black, red and other fungal spots on the surface and inside of the mushroom stick. There is no moldy or sour smell, but a faint mushroom fragrance or sawdust smell, and white, rice-sized bumps appear on the surface of the mushroom stick.

[0055] The method provided by the present invention can shorten the culture period of red-stemmed bamboo fungus sticks from 120 days to 60 days, significantly shortening the culture period of the sticks. At the same time, the method provided by the present invention can control the contamination rate of red-stemmed bamboo fungus sticks to between 1% and 2%, reducing the contamination rate of the sticks.

[0056] Although the present invention has been disclosed above with reference to preferred embodiments, this is not intended to limit the present invention. Any researcher in this field may, without departing from the spirit and scope of the present invention, adopt the design parameters and contents of the above-disclosed embodiments to change and modify the research scheme of the present invention. Therefore, any simple modifications, parameter changes, and modifications made to the above-disclosed embodiments based on the research essence of the present invention without departing from the content of the present invention are within the scope of protection of the present invention.

Claims

1. A method for shortening the culture period of red bamboo fungus sticks, characterized in that: The method includes a method for making a mushroom stick and a method for regulating the key period of mushroom stick growth: (1) Method for preparing mushroom sticks: preparing red-stemmed bamboo fungus culture medium and a filling bag, placing the prepared red-stemmed bamboo fungus culture medium into the prepared filling bag to obtain a mushroom bag material, and then inoculating the mushroom bag material with the fungus species to obtain a mushroom stick; (2) Method for regulating the critical period of mushroom stick growth: The critical period of mushroom stick growth can be regulated by regulating the ambient temperature and humidity during the germination period, as well as the ambient temperature, ambient carbon dioxide concentration and environmental internal circulation during the fever period.

2. The method for shortening the culture period of Dictyophora rubra sticks according to claim 1, wherein: The production of the mushroom sticks is divided into four steps: (1) Preparation of red stem bamboo fungus culture medium Add sawdust, wheat bran, cornmeal, sugar, lime and gypsum into a mixing machine and mix evenly to obtain mixture 1; Water was added to the mixture and the pH was adjusted with lime to obtain mixture 2; Adding humus, humic acid, and glucose to the mixture to obtain mixture 3, which is the red-tubed bamboo fungus culture medium; (2) Production of filling bags Use a plastic bag made of high-temperature resistant polyethylene material, and punch holes in the bag for attaching the breathable patch; (3) Preparation of mushroom bags Weigh the culture medium of Dictyophora rubra and place it into the packing bag prepared in step 2), seal it and use it for subsequent culture; (4) Inoculation of bacterial strains The fermented red stem fungus liquid spawn was inoculated with an inoculating gun, and the inoculation was transferred to a culture room for culture for 60 days to obtain a prepared fungus stick.

3. The method for shortening the culture period of Dictyophora rubra sticks according to claim 2, wherein: The preparation of the red bamboo fungus culture medium in (1) involves the following components, calculated by mass fraction: 60 to 80% sawdust, 15 to 21% wheat bran, 2 to 8% cornmeal, 0.5 to 1% white sugar, 0.3 to 1% lime, and 0.5 to 1.2% gypsum.

4. The method for shortening the culture period of Dictyophora rubra sticks according to claim 2, wherein: In the preparation of the red stem bamboo fungus culture medium, when water is added to the mixture, the ratio of the mixture to water is 1:0.55 to 1:0.65; when the pH is adjusted with lime, the pH range is 7.5 to 8.

2.

5. The method for shortening the culture period of Dictyophora rubra sticks according to claim 2, wherein: When preparing the mixture 3 of the red stem fungus culture medium, the following materials are added into the mixture in the following proportions by mass: 2 to 4% humus, 0.7 to 1.5% humic acid, and 0.7 to 1.5% glucose.

6. The method for shortening the culture period of Dictyophora rubra sticks according to claim 2, wherein: When making the red-stemmed bamboo fungus compost, the size of the packing bag is 16 cm to 19 cm in width and 30 to 35 cm in length, and the punching range is 15*2.3 cm and is located in the middle of the packing belt; the breathable patch is made of high-density polypropylene fiber non-woven fabric with a mass of 80 to 150 grams per square meter, and the length and width of the breathable patch are 2 to 4 cm; the total weight of the red-stemmed bamboo fungus compost after bagging is 1000 to 1500 g.

7. The method for shortening the culture period of Dictyophora rubra sticks according to claim 2, wherein: The preparation of the red stem bamboo fungus culture material is carried out by using fermented red stem bamboo fungus liquid fungus during spawn inoculation, and adopting an inoculation gun to inoculate 30 to 50 ml per fungus stick.

8. The method for shortening the culture period of Dictyophora rubra sticks according to claim 2, wherein: The method for regulating the key period of mushroom stick growth is to regulate the germination period and the fever period.

9. The method for shortening the culture period of Dictyophora rubra sticks according to claim 2, wherein: The method for regulating the key period of mushroom stick growth is as follows: when regulating the germination period, two days before storage, the temperature in the warehouse is adjusted to 30°C and the air humidity is 40%, and maintained for two days; on the day of storage and the second day, the temperature in the warehouse is adjusted to 28°C and the air humidity is 40%; on the third day after storage, the temperature in the warehouse is adjusted to 26°C, and the air humidity is not controlled; on the fourth day after storage, the temperature in the warehouse is adjusted to 24°C, and the air humidity is not controlled; on the fifth day after storage, the temperature in the warehouse is adjusted to 22°C, and the air humidity is not controlled; subsequently, until the fever period, the temperature in the warehouse is maintained at 20°C, and the air humidity is not controlled.

10. The method for shortening the culture period of Dictyophora rubra sticks according to claim 2, characterized in that: The method for regulating the key period of mushroom stick growth is to regulate the fever period. On the 51st to 52nd day after storage, the temperature in the warehouse is adjusted to 20°C, the carbon dioxide concentration is 4500PPM, and the internal circulation in the warehouse is set to ventilate for 15 to 20 minutes per hour. From the 53rd to the 54th day after entering the warehouse, the temperature in the warehouse was adjusted to 19°C, the carbon dioxide concentration was 4000PPM, and the internal circulation in the warehouse was set to 20 to 25 minutes of ventilation per hour; from the 55th to the 56th day after entering the warehouse, the temperature in the warehouse was adjusted to 18°C, the carbon dioxide concentration was 3500PPM, and the internal circulation in the warehouse was set to 20 to 25 minutes of ventilation per hour; from the 57th to the 58th day after entering the warehouse, the temperature in the warehouse was adjusted to 17°C, the carbon dioxide concentration was 3000PPM, and the internal circulation in the warehouse was set to 25 to 30 minutes of ventilation per hour; from the 59th to the 60th day after entering the warehouse, the temperature in the warehouse was adjusted to 16°C, the carbon dioxide concentration was 2500PPM, and the internal circulation in the warehouse was set to 25 to 30 minutes of ventilation per hour.

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