Enzymolysis method and application of cottonseed meal

Abstract

The invention provides an enzymolysis method and application of cottonseed meal, and relates to the technical field of feed additives. Specifically, the method comprises the following steps: adding acid protease, cellulase and pectinase into an acidic cottonseed meal dispersion liquid subjected to ultrasonic treatment, and carrying out first enzymolysis treatment; and adjusting the mixed solution after the first enzymolysis treatment to be alkaline, adding alkaline protease, and carrying out second enzymolysis treatment to obtain the cottonseed meal zymolyte. According to the method, a two-stage enzymolysis process is innovatively adopted and matched with specific enzyme types, macromolecular protein which is difficult to utilize in the cottonseed meal is specifically degraded, the content of small peptide which can be directly absorbed and utilized by livestock is remarkably increased, meanwhile, the peptide chain length and the peptide molecular weight are reduced, the balance of amino acid composition is improved, and the cellulose content is reduced; therefore, the addition amount and the utilization rate of the cottonseed meal in the feed are greatly improved, and the method has a good application prospect.

Description

Technical Field

[0001] The present invention relates to the technical field of feed additives, and in particular to an enzymatic hydrolysis method of cottonseed meal and application thereof. Background Art

[0002] The current feed industry presents unique characteristics. On the one hand, high-energy resources are extremely scarce. For example, corn is significantly unevenly distributed, making it difficult to fully meet the enormous demand for feed production. On the other hand, grain and oil feeds are expensive, making their application in the feed industry difficult. Furthermore, with the booming grain and food industry, the production of roughages such as bran has increased significantly. However, these roughages generally have low digestibility.

[0003] In addition to the aforementioned issues, there is a severe shortage of high-quality, highly absorbable animal protein feeds. Plant-based protein feeds, such as cottonseed meal and other meal products, while widely available and inexpensive, suffer from low utilization rates and contain anti-nutritional factors. Therefore, further research into improving the quality of these inexpensive, high-yield protein feeds, and thereby increasing their inclusion rate and utilization, is of profound significance for the sustainable development of the feed industry and, ultimately, the livestock industry.

[0004] Cottonseed meal, a common plant-based protein feed, has a number of limitations in its use in livestock farming. Firstly, the high average molecular weight of cottonseed meal protein makes it difficult for the livestock digestive system to effectively break it down and absorb it. Secondly, cottonseed meal has a much higher crude fiber content than soybean meal, resulting in a lower usable energy value, significantly reducing energy efficiency.

[0005] Currently, research on cottonseed meal development, both domestically and internationally, focuses on microbial fermentation, leveraging microorganisms to eliminate the anti-nutritional factors and toxic gossypol present in cottonseed meal. However, existing cottonseed meal microbial fermentation methods mostly utilize solid-state fermentation, which presents numerous drawbacks, including extensive fermentation processes, low automation levels, and difficulty in scale-up. Furthermore, the proteases used in cottonseed meal fermentation are ineffective, preventing them from effectively utilizing high-molecular-weight proteins.

[0006] In view of this, the present invention is proposed. Summary of the Invention

[0007] The first purpose of the present invention is to provide an enzymatic hydrolysis method for cottonseed meal to improve the protein quality of cottonseed meal; through the segmented hydrolysis with the help of multiple enzymes, the protein in the cottonseed meal is effectively extracted and converted into feed polypeptides with higher utilization efficiency, laying the foundation for the processing and utilization of cottonseed meal protein.

[0008] The second object of the present invention is to provide a method for processing cottonseed meal.

[0009] The third object of the present invention is to provide a cottonseed meal enzymatic hydrolysate.

[0010] A fourth object of the present invention is to provide a feed.

[0011] In order to achieve the above-mentioned purpose of the present invention, the following technical solutions are adopted:

[0012] A method for enzymatic hydrolysis of cottonseed meal comprises the following steps:

[0013] adding acidic protease, cellulase and pectinase to the acidic cottonseed meal dispersion after ultrasonic treatment to perform a first enzymatic hydrolysis treatment;

[0014] The mixed solution after the first enzymatic hydrolysis treatment is adjusted to alkaline, alkaline protease is added, and a second enzymatic hydrolysis treatment is performed to obtain a cottonseed meal enzymatic hydrolyzate.

[0015] Preferably, the frequency of the ultrasonic treatment is 30 kHz to 50 kHz, and the duration of the ultrasonic treatment is 10 min to 30 min.

[0016] Preferably, for the acidic cottonseed meal dispersion, the mass ratio of cottonseed meal to water is 1:(3-8).

[0017] Preferably, the pH of the acidic cottonseed meal dispersion is 3.5-4.5.

[0018] Preferably, the alkaline pH is 8-10.

[0019] Preferably, based on each gram of cottonseed meal, the dosage of the acidic protease is 400U-1600U, the dosage of the cellulase is 50U-200U, the dosage of the pectinase is 50U-200U, and the dosage of the alkaline protease is 400U-1600U.

[0020] Preferably, the temperature of the first enzymatic hydrolysis treatment and / or the second enzymatic hydrolysis treatment is 40° C. to 60° C., and the time of the first enzymatic hydrolysis treatment and / or the second enzymatic hydrolysis treatment is 8 h to 24 h.

[0021] More preferably, the first enzymatic hydrolysis treatment and / or the second enzymatic hydrolysis treatment is carried out in a shaker, and the shaker oscillation speed is 80 rpm to 120 rpm.

[0022] Preferably, the enzymatic hydrolysis method further comprises post-treatment; the post-treatment comprises heat treatment and crushing treatment, and the temperature of the heat treatment is 102°C to 150°C.

[0023] A cottonseed meal processing method includes the cottonseed meal enzymatic hydrolysis method.

[0024] A cottonseed meal enzymolysate is obtained by the cottonseed meal enzymolysis method.

[0025] A feed comprises the cottonseed meal enzymolysate, or is prepared from the cottonseed meal enzymolysate.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] (1) The present invention provides a segmented enzymatic hydrolysis method for cottonseed meal, which is very efficient in the enzymatic hydrolysis of protein and crude fiber, allowing acidic protease, cellulase and alkaline protease to fully exert their effects under their respective most suitable pH environments, thereby achieving efficient enzymatic hydrolysis of protein and crude fiber in cottonseed meal.

[0028] (2) The present invention introduces ultrasonic cavitation-assisted technology before enzymatic hydrolysis and adopts specific ultrasonic treatment parameters to produce microcracks in the cottonseed meal fiber structure, increase the enzyme action sites, and thus enable cellulase and pectinase to better decompose the intercellular matrix.

[0029] (3) The two-stage enzymatic hydrolysis of the present invention can significantly increase the content of acid-soluble proteins and small peptides in cottonseed meal; among them, acid-soluble proteins and small peptides are more easily absorbed and utilized by the gastrointestinal tract of livestock, and can provide higher-quality and more efficient protein nutrition for the growth and development of livestock, thereby effectively improving the nutritional value and quality of cottonseed meal. At the same time, the present invention can also significantly reduce the crude fiber content in cottonseed meal, increase the available energy value of cottonseed meal, and enhance the applicability and effectiveness of cottonseed meal in livestock feed. DETAILED DESCRIPTION

[0030] The technical solution of the present invention will be clearly and completely described below in conjunction with specific embodiments, but those skilled in the art will understand that the embodiments described below are part of embodiments of the present invention, rather than all embodiments, and are only used to illustrate the present invention, and should not be regarded as limiting the scope of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative work are within the scope of protection of the present invention. If specific conditions are not specified in the embodiments, they are carried out according to conventional conditions or the conditions recommended by the manufacturer. If the manufacturer is not specified for the reagents or instruments used, they are all conventional products that can be purchased commercially. In addition, the terms "first", "second" and "third" are only used for descriptive purposes and are not to be understood as indicating or implying relative importance.

[0031] The first aspect of the present invention is to provide an enzymatic hydrolysis method for cottonseed meal, which mainly comprises the following steps: first, adding acidic protease, cellulase and pectinase to an acidic cottonseed meal dispersion after ultrasonic treatment to perform a first enzymatic hydrolysis treatment; second, adjusting the mixed solution after the first enzymatic hydrolysis treatment to alkaline, adding alkaline protease, and performing a second enzymatic hydrolysis treatment to obtain a cottonseed meal hydrolysate.

[0032] Existing research and development of cottonseed meal as a protein feed has primarily focused on detoxification, increasing protein content, and improving in vitro digestibility, while significantly neglecting in-depth research into the protein degradation mechanisms before and after cottonseed meal fermentation. Furthermore, the enzymatic hydrolysis of cottonseed meal commonly involves mixing various enzymes for hydrolysis, rather than scientifically and rationally segmenting the different enzyme types. This results in the inability to fully utilize the optimal effects of each enzyme.

[0033] The present invention innovatively employs a two-stage enzymatic hydrolysis process to specifically degrade the difficult-to-utilize macromolecular proteins in cottonseed meal, significantly increasing the content of small peptides that can be directly absorbed and utilized by livestock. Simultaneously, it effectively reduces peptide chain length and molecular weight, improves the balance of amino acid composition, and reduces cellulose content, thereby significantly increasing the amount of cottonseed meal added to feed and its utilization rate. The promotion of the present invention's degradation process has far-reaching significance for supplementing protein resources, achieving rational and efficient resource utilization, reducing feed production costs, and promoting the healthy and rapid development of animal husbandry.

[0034] As a preferred embodiment, the frequency of the ultrasonic treatment is 30kHz to 50kHz, and the duration of the ultrasonic treatment is 10min to 30min; in some optional embodiments, the frequency of the ultrasonic treatment includes but is not limited to any one of 30, 32, 35, 38, 40, 42, 45, 48, 50 (kHz) or a numerical range consisting of any two of them, and the duration of the ultrasonic treatment includes but is not limited to any one of 10, 12, 15, 18, 20, 22, 25, 28, 30 (min) or a numerical range consisting of any two of them.

[0035] As a preferred embodiment, the acidic cottonseed meal dispersion comprises cottonseed meal and water, and the mass ratio of cottonseed meal to water is 1:(3-8).

[0036] As a preferred embodiment, the pH of the acidic cottonseed meal dispersion is 3.5-4.5, including but not limited to any one of 3.5, 3.6, 3.7, 3.8, 3.9, 4.0, 4.1, 4.2, 4.3, 4.4, and 4.5, or a numerical range consisting of any two of them.

[0037] As a preferred embodiment, cottonseed meal and water are first mixed, and then subjected to the ultrasonic treatment, and then adjusted to acidity by an acid reagent to obtain the acidic cottonseed meal dispersion after the ultrasonic treatment; in some optional embodiments, the acid reagent includes but is not limited to hydrochloric acid, sulfuric acid, citric acid, acetic acid, humic acid, etc.

[0038] In the present invention, by limiting the solid content, ultrasonic treatment parameters, pH value and other technical characteristics of the acidic cottonseed meal dispersion, the enzymatic hydrolysis effect of the first enzymatic hydrolysis treatment is improved, especially providing a relatively suitable environment for the action of acidic proteases and cellulases. Specifically, the substrate concentration ensures sufficient contact between the cottonseed meal and the enzymes, the cavitation effect, mechanical effect, and thermal effect of the ultrasound can destroy the tissue structure of the cottonseed meal and increase the specific surface area of ​​the cottonseed meal for enzyme interaction, and the pH environment can enhance enzyme activity. In summary, the enzymatic hydrolysis effect is improved and the enzyme cost or time cost of the enzymatic hydrolysis is reduced.

[0039] As a preferred embodiment, per gram of cottonseed meal, the dosage of the acidic protease is 400U-1600U, the dosage of the cellulase is 50U-200U, and the dosage of the pectinase is 50U-200U. In some optional embodiments, the dosage of the acidic protease includes but is not limited to any one of 400, 500, 600, 800, 1000, 1200, 1400, 1500, 1600 (U) or the numerical interval consisting of any two thereof, the dosage of the cellulase includes but is not limited to any one of 50, 60, 80, 100, 120, 150, 180, 200 (U) or the numerical interval consisting of any two thereof, and the dosage of the pectinase includes but is not limited to any one of 50, 60, 80, 100, 120, 150, 180, 200 (U) or the numerical interval consisting of any two thereof.

[0040] As a preferred embodiment, the temperature of the first enzymatic hydrolysis treatment is 40°C to 60°C, and the time of the first enzymatic hydrolysis treatment is 8h to 24h; in some more preferred embodiments, the first enzymatic hydrolysis treatment is carried out in a shaker, and the shaker oscillation speed is 80rpm to 120rpm. In some optional embodiments, the temperature of the first enzymatic hydrolysis treatment includes but is not limited to any one of 40, 42, 45, 48, 50, 52, 55, 58, 60 (°C) or a numerical interval consisting of any two of them, and the time of the first enzymatic hydrolysis treatment includes but is not limited to any one of 8, 10, 12, 15, 18, 20, 22, 24 (h) or a numerical interval consisting of any two of them.

[0041] In the present invention, the enzyme dosage and step parameters of the first enzymatic hydrolysis treatment are restricted to obtain a better enzymatic hydrolysis treatment effect; wherein, the acidic protease can specifically act on the protein in the cottonseed meal and initially decompose it into smaller peptide segments, while the cellulase is responsible for decomposing the cellulose component in the cottonseed meal and reducing the crude fiber content.

[0042] As a preferred embodiment, the alkaline pH is 8 to 10, including but not limited to any one of 8, 8.2, 8.5, 8.8, 9.0, 9.2, 9.5, 9.8, and 10.0, or a numerical range consisting of any two of them.

[0043] As a preferred embodiment, the alkaline reagent used in the adjustment to alkalinity includes but is not limited to sodium hydroxide, potassium hydroxide, calcium hydroxide, etc.

[0044] As a preferred embodiment, the dosage of the alkaline protease is 400 U to 1600 U per gram of cottonseed meal. In some optional embodiments, the dosage of the alkaline protease includes but is not limited to any one of 400, 500, 600, 800, 1000, 1200, 1400, 1500, and 1600 (U), or a numerical range consisting of any two of the following.

[0045] As a preferred embodiment, the temperature of the second enzymatic hydrolysis treatment is 40°C to 60°C, and the time of the second enzymatic hydrolysis treatment is 8h to 24h; in some more preferred embodiments, the second enzymatic hydrolysis treatment is carried out in a shaker, and the shaker oscillation speed is 80rpm to 120rpm. In some optional embodiments, the temperature of the second enzymatic hydrolysis treatment includes but is not limited to any one of 40, 42, 45, 48, 50, 52, 55, 58, 60 (°C) or a numerical interval consisting of any two of them, and the time of the second enzymatic hydrolysis treatment includes but is not limited to any one of 8, 10, 12, 15, 18, 20, 22, 24 (h) or a numerical interval consisting of any two of them.

[0046] In the present invention, the enzyme dosage and step parameters of the second enzymatic hydrolysis treatment are limited to obtain a better enzymatic hydrolysis treatment effect; wherein, after adjusting to an alkaline environment and providing ideal action conditions for alkaline protease, alkaline protease further hydrolyzes the initially decomposed peptide segments into smaller, more easily absorbed peptides.

[0047] As a preferred embodiment, the enzymatic hydrolysis method of the present invention also includes the following post-treatment, which is used to prepare the cottonseed meal enzymatic hydrolysate into a solid state or further powder state for storage, transportation, sale, production and other purposes; specifically, the post-treatment includes heat treatment and optional crushing treatment. In some more preferred embodiments, the heat treatment temperature is greater than 100°C, such as 102°C to 150°C, and the heat treatment time is based on the moisture content being approximately zero; in other more preferred embodiments, the crushing treatment is dry crushing, including but not limited to mechanical crushing, air flow crushing, and ultrasonic crushing, and can be carried out using equipment such as a crusher, ball mill, air flow mill, and vibration mill depending on the production scale.

[0048] A second aspect of the present invention provides a method for processing cottonseed meal, comprising the enzymatic hydrolysis method of cottonseed meal as described in the first aspect. It is understood that the cottonseed meal processing method, in addition to the enzymatic hydrolysis method, may also include any other processing method, including but not limited to physical crushing or screening, puffing, oxidation, chemical reagent treatment, fermentation, and any combination of the above.

[0049] The third aspect of the present invention is to provide a cottonseed meal enzymatic hydrolysate obtained based on the cottonseed meal enzymatic hydrolysis method described in the first aspect.

[0050] The fourth aspect of the present invention is to provide a feed comprising the cottonseed meal hydrolysate as described in the third aspect, or prepared from the cottonseed meal hydrolysate as described in the third aspect. It is understood that, in one aspect, the feed may include the cottonseed meal hydrolysate as one of its ingredients; in another aspect, the feed may be prepared from several raw materials through certain modification processes (with the cottonseed meal hydrolysate as a raw material or one of the raw materials), in which case the feed does not directly include the cottonseed meal hydrolysate as one of its ingredients.

[0051] The cellulase used in the following examples and comparative examples of the present invention is cellulase M4-2 from Wuhan Xinhuayang Biological Co., Ltd., acidic protease is from Hunan Lequ Biotechnology Co., Ltd., and alkaline protease and pectinase are from Dongheng Huadao Biotechnology Co., Ltd.

[0052] Example 1

[0053] S1. Take cottonseed meal, add deionized water 5 times its mass, treat with 40kHz ultrasonic wave for 15 minutes, and then adjust the pH value of the mixture to 4.0 accurately with hydrochloric acid.

[0054] S2. Add 400 U of acidic protease per gram of cottonseed meal, and 50 U of cellulase and pectinase per gram of cottonseed meal. Transfer the mixture to a 50°C water bath shaker, set the speed to 80 rpm, and continue enzymatic hydrolysis for 8 hours.

[0055] S3. After the enzymatic hydrolysis is completed, the pH value is adjusted to 9.0 with ammonia water, and alkaline protease is added at a ratio of 400 U per gram of cottonseed meal. The enzymatic hydrolysis is continued at 80 rpm in a 50°C water bath shaker for 8 hours.

[0056] S4. Drying and crushing the enzymatic hydrolysis product at 105° C. to obtain the enzymatically hydrolyzed cottonseed meal of this embodiment.

[0057] Example 2

[0058] S1. Take cottonseed meal, add deionized water 5 times its mass, treat with 40kHz ultrasonic wave for 15 minutes, and then adjust the pH value of the mixture to 4.0 accurately with hydrochloric acid.

[0059] S2. Add 1600 U of acidic protease per gram of cottonseed meal, and simultaneously add 200 U of cellulase and pectinase per gram of cottonseed meal. Transfer the mixture to a 50°C water bath shaker, set the speed to 120 rpm, and continue enzymatic hydrolysis for 24 hours.

[0060] S3. After the enzymatic hydrolysis is completed, the pH value is adjusted to 9.0 with ammonia water, and alkaline protease is added at a ratio of 1600 U per gram of cottonseed meal. The enzymatic hydrolysis is continued in a 50°C water bath shaker at a speed of 120 rpm for 24 hours.

[0061] S4. Drying and crushing the enzymatic hydrolysis product at 105° C. to obtain the enzymatically hydrolyzed cottonseed meal of this embodiment.

[0062] Example 3

[0063] S1. Take cottonseed meal, add deionized water 5 times its mass, treat with 40kHz ultrasonic wave for 15 minutes, and then adjust the pH value of the mixture to 4.0 accurately with hydrochloric acid.

[0064] S2. Add 800 U of acidic protease per gram of cottonseed meal, and 100 U of cellulase and pectinase per gram of cottonseed meal. Transfer the mixture to a 50°C water bath shaker, set the speed to 100 rpm, and continue enzymatic hydrolysis for 16 hours.

[0065] S3. After the enzymatic hydrolysis is completed, the pH value is adjusted to 9.0 with ammonia water, and alkaline protease is added at a ratio of 800 U per gram of cottonseed meal. The enzymatic hydrolysis is continued in a 50°C water bath shaker at a speed of 100 rpm for 16 hours.

[0066] S4. Drying and crushing the enzymatic hydrolysis product at 105° C. to obtain the enzymatically hydrolyzed cottonseed meal of this embodiment.

[0067] Comparative Example 1

[0068] The method is basically the same as Example 1, except that the addition of acidic protease is omitted.

[0069] Comparative Example 2

[0070] The method is basically the same as Example 1, except that the addition of alkaline protease is omitted.

[0071] Comparative Example 3

[0072] The method is basically the same as Example 1, except that the addition of cellulase and pectinase is omitted.

[0073] Comparative Example 4

[0074] The method is basically the same as Example 1, except that the ultrasonic treatment is eliminated.

[0075] Comparative Example 5

[0076] After adding 10 times the mass of deionized water to the cottonseed meal, 400U of acidic protease, 50U of cellulase, 50U of pectinase, and 400U of alkaline protease were added at one time per gram of cottonseed meal; the system was placed in a 50°C water bath shaker and enzymatically hydrolyzed at 80 rpm for 8 hours. Finally, the system was dried at 105°C and pulverized to obtain the enzymatically hydrolyzed cottonseed meal of this comparative example.

[0077] The cottonseed meal raw material used in the present invention and the cottonseed meal enzymatic hydrolyzed products of various examples and comparative examples were tested to obtain the nutritional indicators shown in Table 1 below.

[0078] Table 1

[0079]

[0080]

[0081] As shown in Table 1, the method for segmented enzymatic hydrolysis of cottonseed meal provided by the present invention can significantly increase the acid-soluble protein content and small peptide content in cottonseed meal and reduce the crude fiber content, thereby improving the quality of cottonseed meal protein and increasing digestibility and utilization.

[0082] By comparing the results of Example 1 with Comparative Examples 1 to 3, it can be seen that the enzyme types used in the present invention are indispensable, and the deletion of any component will affect the effect. The comparison of the results of Example 1 with Comparative Example 4 shows that the ultrasonic pretreatment of the present invention has a positive effect on the effect of the enzyme. The comparison of the results of Example 1 with Comparative Example 5 shows that the segmented enzymatic hydrolysis method of the present invention is significantly superior to the conventional process of directly adding all enzymes at once and enzymatically hydrolyzing.

[0083] Although the present invention has been illustrated and described using specific embodiments, it should be appreciated that the above embodiments are merely intended to illustrate the technical solutions of the present invention rather than to limit them. Those skilled in the art should understand that the technical solutions described in the above embodiments may be modified, or some or all of the technical features thereof may be replaced by equivalents, without departing from the spirit and scope of the present invention. However, these modifications or replacements do not deviate from the essence of the corresponding technical solutions within the scope of the technical solutions of the embodiments of the present invention. Therefore, this means that all such replacements and modifications within the scope of the present invention are included in the appended claims.

Claims

1. A method for enzymatic hydrolysis of cottonseed meal, characterized in that: The steps include: adding acidic protease, cellulase and pectinase to the acidic cottonseed meal dispersion after ultrasonic treatment to perform a first enzymatic hydrolysis treatment; The mixed solution after the first enzymatic hydrolysis treatment is adjusted to alkaline, alkaline protease is added, and a second enzymatic hydrolysis treatment is performed to obtain a cottonseed meal enzymatic hydrolyzate.

2. The enzymatic hydrolysis method of cottonseed meal according to claim 1, wherein The frequency of the ultrasonic treatment is 30kHz to 50kHz, and the duration of the ultrasonic treatment is 10min to 30min.

3. The enzymatic hydrolysis method of cottonseed meal according to claim 1, wherein For the acidic cottonseed meal dispersion, the mass ratio of cottonseed meal to water is 1:(3-8); and / or, the pH of the acidic cottonseed meal dispersion is 3.5 to 4.5; And / or, the alkaline pH is 8-10.

4. The enzymatic hydrolysis method of cottonseed meal according to claim 1, wherein Calculated per gram of cottonseed meal, the dosage of the acidic protease is 400U-1600U, the dosage of the cellulase is 50U-200U, the dosage of the pectinase is 50U-200U, and the dosage of the alkaline protease is 400U-1600U.

5. The enzymatic hydrolysis method of cottonseed meal according to claim 1, wherein The temperature of the first enzymatic hydrolysis treatment and / or the second enzymatic hydrolysis treatment is 40° C. to 60° C., and the time of the first enzymatic hydrolysis treatment and / or the second enzymatic hydrolysis treatment is 8 h to 24 h.

6. The enzymatic hydrolysis method of cottonseed meal according to claim 5, characterized in that: The first enzymatic hydrolysis treatment and / or the second enzymatic hydrolysis treatment is performed in a shaker, and the shaker oscillation speed is 80 rpm to 120 rpm.

7. The enzymatic hydrolysis method of cottonseed meal according to claim 1, wherein The enzymatic hydrolysis method further comprises post-processing; the post-processing comprises heat treatment and crushing treatment, and the temperature of the heat treatment is 102° C. to 150° C.

8. A method for processing cottonseed meal, characterized in that: The invention comprises the enzymatic hydrolysis method of cottonseed meal as claimed in any one of claims 1 to 7.

9. A cottonseed meal enzymatic hydrolysate, characterized in that: The product is obtained by the enzymatic hydrolysis method of cottonseed meal according to any one of claims 1 to 7.

10. A feed, characterized in that The method comprises the cottonseed meal enzymatic hydrolysate as claimed in claim 9, or is prepared from the cottonseed meal enzymatic hydrolysate as claimed in claim 9.

Images