Moisturizing and anti-aging composition for improving skin barrier and preparation method of moisturizing and anti-aging composition

Abstract

The invention relates to a moisturizing and anti-aging composition for improving skin barrier and a preparation method of the moisturizing and anti-aging composition, and particularly discloses a composition which is prepared by scientifically matching active ingredients such as oxyresveratrol and oat kernel protein and acceptable carriers in cosmetics, preferably further compounding phytosterol and nicotinamide, and through the synergistic effect of all the ingredients, the moisturizing and anti-aging effects of the composition are improved. The multiple effects of moisturizing, resisting aging, resisting oxidation and enhancing skin tolerance are achieved, and the technical problems that an existing skin care product is large in irritation, poor in moisturizing effect, weak in skin barrier repairing capacity and the like are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care product preparation, and in particular to a moisturizing and anti-aging composition for improving skin barrier and a preparation method thereof. Background Art

[0002] The skin barrier is a dynamic equilibrium system that maintains skin health. Its functional decline is a key factor in dryness, sensitivity, aging, and other issues. In modern life, factors such as ultraviolet radiation, air pollution, and frequent cleansing can damage the lipid bilayer structure of the stratum corneum, leading to increased transepidermal water loss (TEWL), activating inflammatory signaling pathways, and accelerating collagen degradation.

[0003] Current skincare products face the following technical bottlenecks when addressing barrier repair and anti-aging needs: First, the limitations of traditional active ingredients. While keratin-regulating ingredients like salicylic acid and fruit acid can promote the metabolism of old keratin, their acidic pH disrupts the skin's natural acid-base balance, hindering barrier lipid synthesis. While retinols can promote collagen synthesis, they can easily trigger photosensitivity, limiting their use in anti-aging treatments for sensitive skin. Second, existing products often rely on a single active ingredient to achieve specific efficacy, lacking multi-dimensional synergistic effects. Traditional moisturizers like sodium hyaluronate only form a hydrating film on the surface of the stratum corneum and fail to repair the lipid barrier. While single-ingredient phytosterols can alleviate mild inflammation, their inhibitory effect on the inflammatory factor TNF-α is limited due to the lack of multi-target intervention in inflammatory signaling pathways. Pure antioxidant ingredients like vitamin C are susceptible to photothermal degradation, and their low DPPH free radical scavenging rate makes them ineffective in long-term protection against oxidative stress. Third, the formulation of product components is not scientifically sound, making it difficult for the components to work together to achieve synergistic effects.

[0004] In summary, the development of a composition that is mild and low-irritating, highly effective in moisturizing, anti-aging, and barrier repairing, has become the key to solving the care problems of sensitive skin, dry skin, and barrier-damaged skin. Summary of the Invention

[0005] The present invention provides a moisturizing and anti-aging composition for improving the skin barrier. The composition is prepared by scientifically combining active ingredients such as oxidized resveratrol and oat kernel protein with a carrier acceptable in cosmetics, and preferably further compounding plant sterols and niacinamide. Through the synergistic effect of the ingredients, the composition achieves multiple functions of moisturizing, anti-aging, anti-oxidation and enhancing skin tolerance, thereby solving the technical problems of existing skin care products such as high irritation, poor moisturizing effect and weak skin barrier repair ability.

[0006] To achieve the above object, the present invention adopts the following technical solutions:

[0007] A moisturizing and anti-aging composition for improving skin barrier comprises the following components by mass percentage: 0.1-2% of oxidized resveratrol, 1-8% of oat kernel protein, and a carrier acceptable in cosmetics.

[0008] Furthermore, the moisturizing and anti-aging composition for improving the skin barrier of the present invention further comprises the following components: 0.5-5% of phytosterols and 1-5% of niacinamide.

[0009] As a preferred embodiment, the moisturizing and anti-aging composition for improving the skin barrier of the present invention comprises the following components, by mass percentage: 0.8-1.5% of oxidized resveratrol, 2-4% of phytosterols, 2-5% of oat kernel protein, 2-3.5% of niacinamide, and a carrier acceptable in cosmetics.

[0010] There is no particular limitation on the acceptable carriers in the cosmetics. According to common embodiments in the art, the carriers include but are not limited to one or more combinations of water, glycerin, propylene glycol, butylene glycol, polyethylene glycol, sodium hyaluronate, xanthan gum, and carbomer.

[0011] Furthermore, the phytosterol of the present invention is a mixture of β-sitosterol, stigmasterol and campesterol.

[0012] Preferably, the mass ratio of β-sitosterol, stigmasterol and campesterol is (2-4):(1-3):(1-2).

[0013] The present invention also provides a method for preparing a moisturizing and anti-aging composition for improving the skin barrier, which is characterized by comprising the following steps:

[0014] Add a cosmetically acceptable carrier into a stirring container, stir and heat to 70-85°C to completely dissolve it to obtain material A;

[0015] Mix oxidized resveratrol and phytosterols, stir and heat to 70-85°C to completely dissolve them, then add niacinamide and disperse evenly to obtain material B;

[0016] Under stirring conditions, slowly add material B into material A, and emulsify and stir at a speed of 150-250r / min for 15-30 minutes;

[0017] When the temperature drops to 40-50° C., oat kernel protein is added, and stirring is continued at a speed of 80-120 r / min until uniform, and the mixture is cooled to room temperature to obtain the moisturizing and anti-aging composition for improving the skin barrier.

[0018] On the other hand, the moisturizing and anti-aging composition for improving the skin barrier of the present invention can be used in skin care products.

[0019] A skin care product comprises the aforementioned moisturizing and anti-aging composition for improving the skin barrier, wherein the content of the moisturizing and anti-aging composition for improving the skin barrier in the skin care product is 2-10% by mass.

[0020] Preferably, the dosage form of the skin care product is one of lotion, cream and essence.

[0021] Furthermore, the skin care product further comprises one or more of a thickener, an antioxidant, a moisturizer, an emollient, and a plant extract.

[0022] Based on the excellent moisturizing, anti-aging, and antioxidant effects of the moisturizing and anti-aging composition for improving the skin barrier of the present invention, the present invention also proposes the use of a composition comprising oxidized resveratrol, phytosterols, oat kernel protein, and niacinamide for moisturizing, anti-aging, and enhancing skin tolerance.

[0023] Beneficial effects of the present invention: By compounding oxidized resveratrol, oat kernel protein, phytosterols and niacinamide in specific proportions, a multi-dimensional skin care system is constructed. The synergistic effect of multiple active ingredients not only enables the composition to increase the moisture content of the skin to more than 60% after 28 days of use, significantly reducing the amount of transepidermal water loss, but also reduces the levels of inflammatory factors TNF-α and IL-6 by more than 50%, and the DPPH and ABTS free radical scavenging rates reach more than 85%, significantly improving the skin barrier function and enhancing skin tolerance. In addition, the present invention optimizes the component ratio and preparation process, and is verified to be non-irritating by patch tests. It solves the problem of excessive irritation caused by ingredients such as salicylic acid in existing oil-control and acne-removing products, and achieves the technical effects of efficient moisturizing, anti-aging, and gentle barrier repair. It is suitable for daily skin care for people with sensitive skin, dry skin and skin with damaged barriers. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present invention will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention. The following specific embodiments further describe the present invention.

[0025] The samples of each embodiment and comparative example were prepared according to the weight percentages shown in Tables 1 and 2.

[0026] Table 1

[0027]

[0028]

[0029] The preparation method of the moisturizing and anti-aging composition for improving the skin barrier described in Examples 1-7 comprises the following steps: adding a cosmetically acceptable carrier into a stirring container, stirring and heating to 70°C to completely dissolve it, to obtain material A; mixing oxidized resveratrol and phytosterols, stirring and heating to 70°C to completely dissolve it, and then adding niacinamide to disperse it evenly to obtain material B; under stirring conditions, slowly adding material B to material A, and emulsifying and stirring at a speed of 250 r / min for 30 minutes; when the temperature drops to 40°C, adding oat kernel protein, continuing to stir at a speed of 100 r / min until uniform, and cooling to room temperature to obtain the moisturizing and anti-aging composition.

[0030] Table 2

[0031]

[0032] The sample preparation steps of Comparative Examples 1-9 are basically the same as those of Examples 1-7, with the only difference being the types and amounts of certain raw materials.

[0033] The performance of each sample was tested according to the following method.

[0034] Skin Moisture Content Test: 80 volunteers aged 25-45 years with mildly impaired skin barrier were randomly divided into 16 groups, with 5 participants in each group. The compositions of Examples 1-7 and Comparative Examples 1-9 were applied once daily, morning and evening, for 28 consecutive days. The moisture content of the volunteers' inner forearms was measured using a skin moisture meter (Corneometer CM825, CK, Germany) before use and on days 7, 14, 21, and 28 after use. The results are shown in Table 3.

[0035] Transepidermal water loss (TEWL) test: At the same time as the above-mentioned skin moisture content test, the TEWL value of the skin on the inner forearm of the volunteers was measured using a transepidermal water loss tester (TewameterTM300, CK Company, Germany). The results are shown in Table 4.

[0036] Skin Inflammatory Factor Detection: 30 volunteers with sensitive skin were randomly divided into 6 groups, each with 5 participants. They were treated with the compositions of Examples 4 and 6 and Comparative Examples 1, 3, 6, and 8, once daily, morning and evening, for 28 consecutive days. Skin samples were collected before and after 28 days of use, and the levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the skin were measured using enzyme-linked immunosorbent assay (ELISA). The results are shown in Table 5.

[0037] DPPH free radical scavenging experiment: Prepare a 0.1mM DPPH ethanol solution and store in the dark. Dilute the compositions of Examples 4 and 6 and Comparative Examples 1, 3, 6, and 8 to 1 mg / mL with ethanol to serve as the sample group. A 0.1mM vitamin C solution was used as the positive control group, and an ethanol blank group was used. Mix 200μL of the sample solution with 200μL of the DPPH solution, incubate in the dark for 30 minutes, and measure the absorbance at 517nm (Asample). Simultaneously measure the absorbance of the sample solution and ethanol mixture (Ablank) and the absorbance of the DPPH solution and ethanol mixture (Acontrol). Calculate the scavenging rate according to the following formula.

[0038] Clearance rate (%) = [1-(A sample-A blank) / A control] x 100%.

[0039] ABTS free radical scavenging assay: First, mix equal volumes of 7 mM ABTS solution and 2.45 mM potassium persulfate solution. Incubate in the dark for 12-16 hours to prepare an ABTS stock solution. Dilute with PBS to an absorbance of 0.7 ± 0.02 at 734 nm to prepare a working solution. Mix 100 μL of sample solution with 900 μL of working solution and incubate at room temperature in the dark for 6 minutes. Measure the absorbance at 734 nm (B sample). Simultaneously, measure the absorbance of a mixture of 100 μL sample solution and 900 μL PBS (B blank) and a mixture of 100 μL ethanol and 900 μL working solution (B control). Calculate the scavenging rate according to the following formula.

[0040] Clearance rate (%) = [1-(B sample-B blank) / B control] × 100%

[0041] Table 3 Test results of skin moisture content of subjects before and after using the composition of the present invention

[0042]

[0043]

[0044] Table 4 Transepidermal water loss test results of subjects before and after using the composition of the present invention

[0045]

[0046] As shown in the experimental results of Tables 3 and 4, the composition of the present invention exhibits excellent sustained moisturizing ability. After 28 days of use, the skin moisture content can reach up to 61.5%, which is significantly better than each comparative example. In the present invention, oxidized resveratrol provides strong antioxidant protection and reduces environmental damage to the skin barrier; phytosterols (especially β-sitosterol, stigmasterol, and campesterol mixed in a specific ratio) effectively simulate and supplement the skin's natural lipids and strengthen the barrier structure; oat kernel protein exerts excellent hydrophilic and hygroscopic properties and increases the water content of the stratum corneum; niacinamide promotes the synthesis of barrier lipids such as ceramides in the skin itself and enhances its water-locking ability. The four active ingredients cooperate with each other under the preferred ratio, not only each of which plays its core effect, but also significantly improves the overall moisturizing efficiency and durability of the composition through synergistic effects, by repairing the skin barrier, maintaining the integrity of the stratum corneum structure, and delaying the decline of skin barrier function. In contrast, the lack of any core component (such as comparative examples 1-4) or the imbalance of the ratio (such as comparative examples 5-7) all lead to a significant decrease in the moisturizing effect or even ineffectiveness. The plant sterol dosage ratio in Comparative Examples 8-9 is not in the optimal range. Although the moisturizing effect of the composition is improved compared with other comparative examples, it is still inferior to the examples of the present invention as a whole.

[0047] Table 5 Detection results of skin inflammatory factors of subjects before and after using the composition of the present invention

[0048]

[0049] As can be seen from the results in Table 5, the composition of the present invention exhibits excellent synergistic effects in inhibiting skin inflammation. Its core advantage lies in the fact that the four active ingredients construct a multi-pathway anti-inflammatory system. As shown in Example 6, the levels of TNF-α and IL-6 were significantly reduced by 60.5% and 57.1%, respectively. In contrast, in Comparative Examples 1 and 3, which lack core ingredients, or Comparative Examples 6 and 8, which have an unbalanced component ratio, the levels of TNF-α and IL-6 reduction were significantly lower than those in Examples 4 and 6. The possible reason is that oxidized resveratrol and nicotinamide can target and inhibit signal pathways, blocking the release of core inflammatory factors, and phytosterols intervene in inflammatory signal transduction by downregulating receptor expression. The natural anti-inflammatory substances in oat kernel protein have the effect of clearing inflammatory triggering factors. The scientific combination of the components of the present invention gives the composition excellent anti-inflammatory efficacy, and has a more obvious improvement effect on symptoms such as redness and tingling of sensitive skin. Inhibiting inflammatory reactions can reduce the activation of matrix metalloproteinases, avoid collagen fiber degradation, and thus delay skin aging.

[0050] Table 6 Free radical scavenging performance test results of the composition of the present invention

[0051]

[0052]

[0053] As can be seen from the results in Table 6, the composition of the present invention exhibits significant advantages in antioxidant performance. Oxidized resveratrol acts as a powerful antioxidant to neutralize free radicals, and phytosterols significantly delay the degradation of oxidized resveratrol by stabilizing the structure of the active ingredient; nicotinamide enhances the activity of superoxide dismutase and strengthens the cell's own antioxidant capacity; the polyphenols in oat kernel protein also have strong antioxidant effects. As shown in Example 6, the DPPH and ABTS free radical scavenging rates reached 85.2% and 88.6%, respectively, which are significantly higher than those of the control examples. The composition of the present invention exhibits a more significant anti-aging effect by efficiently scavenging free radicals, reducing the damage of oxidative stress to skin cells, and delaying the loss of collagen.

[0054] Patch test: The samples of Examples 1-7 were prepared according to the components and amounts shown in Table 1, and the irritation of the samples was tested by patch test.

[0055] The samples to be tested were added dropwise to the filter paper attached to the spot tester and placed in the spot tester. The spot tester was then applied to the cheek of the subject using non-irritating tape. Gently press with the palm of your hand to ensure that it is evenly adhered to the skin surface. The application period was 24 hours. After application, the spot tester was removed and the indentation was allowed to completely disappear for 30 minutes. The skin reaction was observed for the first time, and the second and third observations were recorded 24 hours and 48 hours after removal, respectively. Each sample was tested independently on the faces of three different subjects with similar skin conditions. The final result was the highest skin reaction level observed in the three tests. The subject groups used in all examples did not overlap.

[0056] A blank group was set up: deionized water of the same mass as that of the composition in Example 1 was weighed as a blank sample, and a patch test was performed according to the above method. The results are shown in Table 3.

[0057] The evaluation criteria for skin reactions to the patch test are shown in Table 7, and the test results are shown in Table 8.

[0058] Table 7

[0059]

[0060]

[0061] Table 8 Patch test results

[0062]

[0063] The above embodiments are preferred implementation schemes of the present invention. In addition, the present invention can also be implemented in other ways. Any obvious replacement without departing from the concept of the present invention is within the scope of protection of the present invention.

Claims

1. A moisturizing and anti-aging composition for improving skin barrier, characterized in that: Calculated by mass percentage, the composition comprises the following components: 0.1-2% of oxidized resveratrol, 1-8% of oat kernel protein, and a carrier acceptable in cosmetics.

2. The moisturizing and anti-aging composition for improving the skin barrier according to claim 1, characterized in that: Calculated by mass percentage, the invention also includes the following components: 0.5-5% of phytosterols and 1-5% of nicotinamide.

3. The moisturizing and anti-aging composition for improving skin barrier according to claim 2, characterized in that: The composition comprises the following components by mass percentage: 0.8-1.5% of oxidized resveratrol, 2-4% of phytosterol, 2-5% of oat kernel protein, 2-3.5% of nicotinamide, and a carrier acceptable in cosmetics.

4. The moisturizing and anti-aging composition for improving skin barrier according to claim 1, characterized in that: The acceptable carriers in the cosmetics include, but are not limited to, one or more combinations of water, glycerin, propylene glycol, butylene glycol, polyethylene glycol, sodium hyaluronate, xanthan gum, and carbomer.

5. The moisturizing and anti-aging composition for improving skin barrier according to claim 1, characterized in that: The phytosterol is a mixture of β-sitosterol, stigmasterol and campesterol, and the mass ratio of β-sitosterol, stigmasterol and campesterol is (2-4):(1-3):(1-2).

6. The method for preparing the moisturizing and anti-aging composition for improving the skin barrier according to any one of claims 1 to 5, characterized in that: The following steps are involved: Add a cosmetically acceptable carrier into a stirring container, stir and heat to 70-85°C to completely dissolve it to obtain material A; Mix oxidized resveratrol and phytosterols, stir and heat to 70-85°C to completely dissolve them, then add niacinamide and disperse evenly to obtain material B; Under stirring conditions, slowly add material B into material A, and emulsify and stir at a speed of 150-250r / min for 15-30 minutes; When the temperature drops to 40-50° C., oat kernel protein is added, and stirring is continued at a speed of 80-120 r / min until uniform, and the mixture is cooled to room temperature to obtain the moisturizing and anti-aging composition for improving the skin barrier.

7. A skin care product, characterized in that: The moisturizing and anti-aging composition for improving the skin barrier according to any one of claims 1 to 6 is contained in the skin care product in an amount of 2-10% by mass.

8. The skin care product according to claim 7, characterized in that The dosage form of the skin care product is one of lotion, cream and essence.

9. The skin care product according to claim 7, characterized in that The skin care product further comprises one or more of a thickener, an antioxidant, a moisturizer, an emollient, and a plant extract.

10. Use of a composition comprising oxidized resveratrol, phytosterols, oat kernel protein and niacinamide for moisturizing, anti-aging and enhancing skin tolerance.

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