Hair care composition with multi-effect repairing effect and application thereof

Abstract

The invention relates to a hair care composition which has the repairing effects of moisturizing and oil control, the composition comprises a yeast fermentation product, an aspergillus fermentation product and a plant extract, the plant extract comprises a persimmon fruit extract and a ginger root extract, the yeast fermentation product comprises a freeze-dried product of yeast fermentation lysate filtrate, and the aspergillus fermentation product comprises a lyophilized product of yeast fermentation lysate filtrate. The composition provided by the invention can effectively inhibit scalp grease secretion.

Description

Technical Field

[0001] The present invention relates to the technical field of biological preparation compositions, and in particular to a preparation method of a hair care composition with moisturizing and oil-controlling repairing effects and its application in hair care. Background Art

[0002] With the development of modern hair care products and the updating of scalp cosmetics, as well as the pollutants in the living environment, more and more people are experiencing problems such as excessive scalp oil secretion, dandruff, and scalp sensitivity, which has gradually increased consumers' demand for the effectiveness of hair care products. Among them, the imbalance of scalp oil secretion has led to more consumers having a more urgent demand for the oil control effect of hair care products. In the past few decades, several methods have been used to measure the sebum secreted on the skin surface, such as photometry technology, strip technology or a combination of concentrated technologies.

[0003] In the research and development of multi-effect hair care products, CN112137920A discloses a yeast fermentation lysate, its preparation method, and application. The extraction method of the yeast fermentation lysate includes yeast fermentation and proliferation, obtaining yeast bodies, preparing yeast concentrate, and obtaining yeast fermentation lysate. When used in a hair care composition, the disclosed yeast fermentation lysate can significantly improve keratin fibers, has a conditioning effect on hair, can increase hair gloss and touch, and repair damaged hair. CN112494418A discloses a Chinese medicine fermentation shampoo and its preparation method, comprising: deionized water, ammonium laureth sulfate, sodium lauroyl glutamate, decyl glucoside, yeast fermentation product filtrate, lactobacillus fermentation product, aspergillus fermentation product, p-hydroxyacetophenone, hexylene glycol, ginger water, Polygonum multiflorum root water, Platycladus orientalis leaf extract, Gleditsia microcarpa fruit extract, Sapindus mukorossi peel extract, jojoba seed oil, Nasturtium officinale extract, Melaleuca alternifolia oil, Panax ginseng root extract, Cinchona pubescens bark extract, Lonicera japonica extract, and lemon peel oil. The shampoo can soften and nourish hair, prevent split ends, and provide a one-stop shampoo and conditioner, thereby leaving hair refreshed and non-greasy, with a sense of movement and elegance. However, there is still much room for exploration in developing natural plant extracts for controlling scalp oiliness and hair care.

[0004] The main purpose of the present invention is to develop a multi-effect hair care biological composition containing persimmon fruit extract and use the composition in the development of hair care products. Summary of the Invention

[0005] The present invention aims to provide a hair care composition with multi-effect repairing effects and its application.

[0006] Persimmons, also known as red persimmons or red fruits, are the fruit of the persimmon tree, renowned for their sweet taste and rich nutritional value. Persimmons are divided into sweet and astringent varieties. Sweet persimmons can be eaten directly, while astringent persimmons require a de-astringency treatment. The flavonoids and tannins in persimmon fruit extract contain ortho-phenolic hydroxyl groups, which can form complexes with metal ions, thereby affecting the growth of microorganisms. The antibacterial effects of different extraction methods and extract components may vary. Persimmon fruit extract, also known as persimmon extract, is extracted from the persimmon fruit and has a variety of applications, including in cosmetics, health supplements, and deodorants. Its main component is persimmon tannin, which has antioxidant, antibacterial, anti-inflammatory, hemostatic, and deodorizing properties.

[0007] Ginger root extract, also known as ginger extract, is extracted from the rhizome of Zingiber officinale (Zingiber officinale), a plant of the Zingiberaceae family. Its main components include zingiberene, gingerol, bisabolene, α-curcumene, and methylheptenone. Ginger root extract has various medicinal and health benefits, such as dispelling cold, warming the middle and stopping vomiting, resolving phlegm and relieving cough, and serving as an anti-inflammatory and antioxidant.

[0008] Yeast is one of the most important microorganisms commercially exploited by humans. The fermentation product filtrate of yeast contains natural active ingredients and is an excellent comprehensive skin conditioner. Yeast fermentation products are diverse and include alcohol, carbon dioxide, organic acids, various flavor compounds, proteins, polyphenols, vitamins, minerals, amino acids, polysaccharides, and fiber. These products are widely used in food, beverages, medicine, cosmetics, and other fields.

[0009] One aspect of the present invention provides a hair care composition, characterized in that the composition has moisturizing and oil-control repairing effects, and the composition comprises a yeast fermentation product, an Aspergillus fermentation product and a plant extract, and the plant extract includes a persimmon fruit extract and a ginger root extract.

[0010] Furthermore, the components of the composition include, by weight, 0.5-5 parts of yeast fermentation product, 0.2-2 parts of Aspergillus fermentation product, and 1-10 parts of plant extract.

[0011] Furthermore, the mass ratio of the persimmon fruit extract to the ginger root extract is 1-5:0.5-2.

[0012] Furthermore, the yeast fermentation product includes a freeze-dried product of yeast fermentation lysate filtrate.

[0013] Furthermore, the preparation method of the freeze-dried product of the yeast fermentation lysate filtrate is as follows: the preparation method of the freeze-dried product of the yeast fermentation lysate filtrate is as follows: the coated yeast (SACCHAROMYCOPSIS, purchased from Beina Bio) is inoculated into a composite fermentation medium (cell concentration is 1×10 7CFU / mL, inoculation size is 10%), placed in a 28°C-30°C incubator for fermentation and proliferation for 48-72 hours, centrifuged at 4000-5000 rpm for 30-40 min, the precipitate was taken, ultrasonically disrupted (ultrasonic power is 450-600w, 30-40 min, time interval is 5s), centrifuged at 8000-10000 rpm for 30 min, the supernatant was taken, and concentrated by 200kDa-2000kDa ultrafiltration. The retained filtrate was the yeast fermentation lysate filtrate, which was freeze-dried and concentrated.

[0014] Furthermore, the composite fermentation medium contains an extract and nutrients, wherein the extract is the supernatant of persimmon fruit extract mixed with water and ultrasonically extracted (ultrasonic power of 50-80w, 5-10min). The composite fermentation medium is sterilized before use. The nutrients include 0.5-1.0% by weight of glucose and 1.0-2.0% by weight of peptone. The sterilization method includes, but is not limited to, conventional sterilization methods such as high-pressure steam sterilization, filtration sterilization, and pasteurization.

[0015] Furthermore, the mass percentage of the extract is 1.5-5.5%.

[0016] Another aspect of the present invention provides use of the aforementioned hair care composition in preparing a moisturizing and oil-controlling hair care product.

[0017] Another aspect of the present invention provides use of the aforementioned hair care composition in preparing a moisturizing and oil-controlling hair care product.

[0018] Another aspect of the present invention provides a hair care product comprising the aforementioned hair care composition.

[0019] Furthermore, the product also includes at least one of a chelating agent, a preservative, an adsorbent, and a soothing agent.

[0020] The beneficial effects of the present invention are:

[0021] (1) The present invention uses a combination of yeast fermentation products, Aspergillus fermentation products, and plant extracts to prepare a hair care composition. The composition of the present invention can effectively inhibit scalp oil secretion.

[0022] (2) The method for preparing the yeast fermentation product of the present invention can obtain a fermentation product with high oil control efficiency, which has obvious advantages.

[0023] (3) The hair care composition of the present invention can effectively control oil and increase the water content of the scalp skin after being prepared into a hair care product. DETAILED DESCRIPTION

[0024] The embodiments of the present invention provide a composition with moisturizing, oil-controlling and repairing effects and its application in hair care. In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. Its purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Unless otherwise specified, the experimental reagents and instruments designed for the implementation and comparative examples of the present invention are all commonly used ordinary reagents and instruments, which can be obtained from commercial channels. In the implementation and comparative examples, the experimental methods used are all conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch of raw materials.

[0025] Example 1 This example provides the preparation of a freeze-dried product of a yeast fermentation lysate filtrate.

[0026] The preparation method of the freeze-dried product of yeast fermentation lysate filtrate is as follows: the coated yeast (SACCHAROMYCOPSIS, purchased from Beina Biotechnology) was inoculated into the composite fermentation medium (cell concentration was 1×10 7 CFU / mL, inoculation size is 10%), placed in a 28°C incubator for fermentation and proliferation for 60 hours, centrifuged at 4500 for 40 minutes, took the precipitate, ultrasonically disrupted (ultrasonic power is 500w, 40min, time interval 5s), centrifuged at 10000rpm for 30min, took the supernatant, ultrafiltration and concentration (the retention molecular weight of the ultrafiltration membrane is 1000kDa), and the retained filter is the yeast fermentation lysate filtrate, which is freeze-dried and concentrated. Among them, the composite fermentation medium is a sterilized medium, which contains an extract and nutrients. The extract is the supernatant extracted by ultrasonication (ultrasonic power is 80w, 10min) after mixing persimmon extract with water. The nutrients include glucose and peptone, the mass percentage of glucose is 0.5%, the mass percentage of peptone is 1.0%, the mass percentage of the extract is 4.5%, and the weight ratio of persimmon extract to water in the extract is 1:5

[0027] Comparative Examples 1-5

[0028] See Example 1, the difference is that the composite fermentation medium does not contain the extract, and the mass percentages of the extract are 1.5%, 2.5%, 3.5%, and 5.5% respectively.

[0029] Comparative Examples 6-7

[0030] See Example 1, the difference is that the weight ratio of persimmon fruit extract to water in the extract is 1:2.5, and the weight ratio of persimmon fruit extract to water in the extract is 1:10.

[0031] The yeast fermentation products (lyophilized products of yeast fermentation lysate filtrate) obtained in Example 1 and Comparative Examples 1-7 were formulated into hair care compositions, wherein the components, counted by weight, included: 5 parts of yeast fermentation product, 0.5 parts of Aspergillus fermentation product, and 5 parts of plant extract (the mass ratio of persimmon fruit extract and ginger root extract was 1:0.5). The above composition was formulated into 12.5, 25, and 50 μg / mL solutions using physiological saline.

[0032] Effect measurement

[0033] SZ95 cells were activated and cultured in a CO2 cell culture incubator. Cells with good viability were selected for the experiment. SZ95 cells were seeded into 6-well plates at a cell density of approximately 1×10 5 / mL, 500μL of the above-mentioned sample to be tested was added to each well, and then induced with 100μM oleic acid complete cell culture medium (1500μL). After 72h of culture, the SZ95 cells were collected, washed 3 times with PBS, fixed with 4% formaldehyde for 10min, washed 3 times with PBS, and washed again with 60% isopropanol 3 times. Then, the SZ95 cells were stained with Oil Red O dye for 30min, washed twice with PBS, and counterstained with hematoxylin. The cell culture plate was placed under a microscope to observe the lipid droplet distribution of the SZ95 cells. The oil red staining area was calculated using Image J software and the results were recorded. See Table 1. Experimental setting blank control (500μL saline + 1500μL complete culture medium, average oil red staining area 0.41±0.15μm 2 ) and oleic acid-induced model group (500 μL normal saline + 100 μM oleic acid complete medium 1500 μL, average oil red staining area 3.98 ± 0.24 μm 2 ).

[0034] Table 1. Comparison of lipid content in SZ95 cells of each group (%, n=3)

[0035]

[0036] Compared to the blank group, the lipid content of SZ95 cells in the model group increased significantly, with the difference being statistically significant. As shown in Table 1, the composition comprising a yeast fermentation product, an Aspergillus fermentation product, and a plant extract of the present invention significantly reduced the lipid content of SZ95 cells compared to the model group. Notably, the hair care composition formulated with a yeast fermentation product obtained by culturing coated yeast with an extract, an Aspergillus fermentation product, and a plant extract exhibited significantly better inhibitory effects than a composition formulated with a yeast fermentation product obtained by culturing coated yeast without an extract.

[0037] Example 2 Composition Preparation

[0038] This example provides a hair care composition comprising a yeast fermentation product (a freeze-dried product of a yeast fermentation lysate filtrate), an Aspergillus fermentation product, and a plant extract, wherein the plant extract includes a persimmon fruit extract and a ginger root extract. The yeast fermentation product is prepared as described in Example 1. The Aspergillus fermentation product and the ginger root extract are purchased from Bio-Bio Biotechnology Co., Ltd., and the persimmon fruit extract is purchased from Tianjin Fancui Biopharmaceutical Technology Co., Ltd. The proportions (parts by weight) of the components are shown in Table 2.

[0039] Table 2. Preparation table

[0040]

[0041] Referring to Example 1, the above composition was prepared into a 25 μg / mL solution using physiological saline, and the lipid assay of SZ95 cells was performed. The results are shown in Table 3.

[0042] Table 3. Comparison of lipid content in SZ95 cells of each group (%, n=3)

[0043]

[0044]

[0045] As can be seen from Table 3, on the basis of the combination of yeast fermentation products and plant extracts, the addition of Aspergillus fermentation products and ginger root extract all had a certain inhibitory effect on the oil content of SZ95 cells, and the ginger root extract and its combination had a significant synergistic effect. The best effect was achieved when 5 parts of yeast fermentation products, 1 part of Aspergillus fermentation products, 5 parts of persimmon fruit extract and 1 part of ginger root extract were added.

[0046] Example 3

[0047] The above composition 8 was prepared into a sample with the following formula (w / w, %): 85% composition, 8% vinyl dimethicone / polymethicone silsesquioxane crosspolymer, 3% lithium magnesium sodium silicate, 10% ethanol, and the balance water. A commercially available hair care oil control product (Izumihou Lvou) was purchased as a control.

[0048] Performance Test 1

[0049] Thirty subjects with high scalp oil secretion were recruited and voluntarily signed informed consent forms. They were aged between 20 and 40 years old. They were divided into two groups (test group and commercial group), with 15 people in each group. They did not wash their hair the night before the test and did not comb their hair as much as possible on the test day. The scalp oil test was performed using the Sebumeter method. A 0.1mm thick pad (German CK) was placed in contact with the scalp. The lipid compounds in the scalp caused the transparency of the pad to change. The test pad was then flattened and placed on the slide of the instrument and pushed into the tester. The transmittance distribution curve on the tape was obtained by testing with the photoelectric tube in the instrument. The peak of the transmittance curve distribution represents the content of lipid compounds, i.e., oil, in the scalp. The subjects were self-controlled. The head was divided in the middle along the hairline. The left side was the blank control, which was applied with clean water, and the right side was the test group, which was applied with the test composition sample. An area of ​​approximately 3cm×3cm from the forehead to the top of the head was designated as the test site. The test substance was wiped on the scalp and allowed to dry naturally. The test was continued for 7 days (washing the hair on the 3rd day and not washing the hair on the rest of the time). The basal oil production of the scalp of the subjects was measured before the start of the test experiment. The oil content was tested three times at the test site on the first day of the test and 24 hours after the last application of the test substance. The results are shown in Table 3.

[0050] Table 4 Determination of oil content (μg / cm 2 )

[0051]

[0052] As shown in Table 4, before using the sample, the scalp oil content of the two groups of testers was taken as the baseline value, and there was no significant difference between the two groups. After one day of using the sample, the scalp oil content began to show significant suppression, with a mean difference of 9.2μg / cm 2 , after 7 days the difference increased to 19.6 μg / cm 2 The inhibitory effect is far better than that of commercial products, indicating that this product can effectively and quickly clean scalp oil, and the effect is obvious when used in the short term. In addition, no adverse skin reactions occurred among the test subjects.

[0053] Performance Test 2

[0054] Skin hydration was measured in the previously recruited subjects using a three-probe skin water loss test system (CK, Germany). Three replicates were measured, covering the previously described test areas, and the average value was calculated. Hydration was measured before sample application, designated T0. Hydration was measured at the same location on day 1 and day 7, designated T1. Hydration was calculated as (T1 - T0) / T0. Results are shown in Table 5.

[0055] Table 5 Skin hydration rate (%)

[0056] Group 1 day of use 7 days of use Test samples 13 15 Commercially available products 10 8

[0057] As can be seen from Table 5, the composition of the present invention has a significant advantage in terms of skin water retention, and can even increase the water content of the skin, with an effect better than commercially available products.

[0058] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same and similar parts between the various embodiments can be referred to each other. The above description of the disclosed embodiments enables professionals and technicians in this field to implement or use the present invention. Various modifications to these embodiments will be apparent to professionals and technicians in this field, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A hair care composition, characterized in that The composition has moisturizing and oil-control repairing effects. The composition comprises a yeast fermentation product, an Aspergillus fermentation product, and a plant extract. The plant extract comprises a persimmon fruit extract and a ginger root extract. The yeast fermentation product comprises a freeze-dried product of a yeast fermentation lysate filtrate. The preparation method of the freeze-dried product of the yeast fermentation lysate filtrate comprises the following steps: inoculating a film-coated yeast into a composite fermentation medium, placing the product in an incubator at 28° C.-30° C. for fermentation and proliferation for 48-72 hours, centrifuging the product at 4000-5000 rpm for 30-40 minutes, collecting a precipitate, ultrasonically crushing the product, centrifuging the product at 8000-10000 rpm for 30 minutes, collecting a supernatant, and concentrating the product through 200 kDa-2000 kDa ultrafiltration. The retained filtrate is the yeast fermentation lysate filtrate, and the product is freeze-dried and concentrated.

2. The composition according to claim 1, characterized in that The components of the composition include, by weight, 0.5-5 parts of yeast fermentation product, 0.2-2 parts of Aspergillus fermentation product, and 1-10 parts of plant extract.

3. The composition according to claim 1, characterized in that The mass ratio of the persimmon fruit extract to the ginger root extract is 1-5:0.5-2.

4. The composition according to claim 3, characterized in that The composite fermentation medium contains an extract and nutrients, and the extract is the supernatant obtained by ultrasonic extraction after the persimmon fruit extract is mixed with water.

5. The composition according to claim 4, characterized in that The mass percentage of the extract in the culture medium is 1.5-5.5%.

6. Use of the hair care composition according to claim 1 in preparing a moisturizing and oil-controlling hair care product.

7. Use of the hair care composition according to claim 1 in preparing a moisturizing and oil-controlling hair care gel.

8. A hair care product, characterized in that The composition according to claim 5.

9. The hair care product according to claim 8, characterized in that It also includes at least one of a chelating agent, a preservative, an adsorbent, and a soothing agent.

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