Recombinant human collagen oral cavity restoration dressing
By preparing an oral repair dressing containing recombinant human collagen, the problem that existing oral antibacterial agents cannot effectively heal wounds is solved, and the effects of rapid pain relief and skin healing promotion are achieved.
Patent Information
- Application Number
- CN202510940063.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-08
- Publication Date
- 2025-09-26
AI Technical Summary
Existing oral antibacterial agents have limited effects in relieving oral ulcers and pain and cannot effectively promote sore healing.
Recombinant human collagen oral repair dressing is used, which contains recombinant human collagen, carbomer, triethanolamine, glycerol, dimethyl sulfoxide, ethylparaben and vitamin C. It is prepared by mixing and adding water to form a dressing with antibacterial and anti-inflammatory effects and the ability to quickly repair wounds.
Recombinant human collagen dressing significantly improves the speed of skin repair and has the effect of quickly relieving pain and healing wounds. Carbomer and ethylparaben provide anti-inflammatory and emulsification stabilization effects, triethanolamine and glycerin moisturize and hydrate, and dimethyl sulfoxide enhances drug penetration, forming a protective layer to promote healing.
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Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of oral dressings, in particular to a recombinant human collagen oral repair dressing. Background Art
[0002] The oral cavity is one of the main pathways for bacteria, viruses, fungi and other foreign antigens to enter the human body. In addition to this exogenous burden, there are also a large number of endogenous microbial flora parasitic in the oral cavity, teeth and mucosal surfaces. The clustering of microbial colonies causes problems such as oral ulcers to occur frequently.
[0003] Existing oral antibacterial agents, primarily composed of carbomer, triethanolamine, disodium EDTA, sodium propylparaben, and water, can alleviate pain from oral ulcers, oral inflammation, denture wounds, and surgical wounds. However, while the carbomer present in these agents can kill bacteria and relieve pain, it does not directly aid wound healing. Consequently, spraying these oral antibacterial agents on oral ulcers only provides temporary pain relief. Therefore, we propose a recombinant human collagen oral restorative dressing. Summary of the Invention
[0004] The present invention provides a recombinant human collagen oral repair dressing, which has the advantages of clearing away heat and detoxifying, antibacterial and anti-inflammatory, and can quickly repair the skin at the wound, thereby solving the problems raised in the above-mentioned background technology.
[0005] The technical solution of the present invention is achieved as follows: a recombinant human collagen oral repair dressing is designed, which includes recombinant human collagen, carbomer, triethanolamine, glycerol, dimethyl sulfoxide, ethyl paraben, vitamin C and pure water.
[0006] Preferably, the preparation method of the recombinant human collagen oral repair dressing is as follows: Step 1: Mix recombinant human collagen, carbomer, triethanolamine, glycerol, dimethyl sulfoxide, ethylparaben, and vitamin C together; Step 2: Then stir and mix with purified water to prepare the recombinant human collagen oral repair dressing.
[0007] Preferably, the recombinant human collagen oral repair dressing comprises the following components by weight: 2-18 parts of recombinant human collagen, 2-12 parts of carbomer, 1-10 parts of triethanolamine, 1-8 parts of glycerol, 2-12 parts of dimethyl sulfoxide, 1-10 parts of ethylparaben, and 0.5-3 parts of vitamin C.
[0008] Preferably, 2 parts of recombinant human collagen, 2 parts of carbomer, 1 part of triethanolamine, 1 part of glycerol, 2 parts of dimethyl sulfoxide, 1 part of ethylparaben, and 0.5 parts of vitamin C.
[0009] Preferably, the ingredients include 4.5 parts of recombinant human collagen, 4 parts of carbomer, 2.5 parts of triethanolamine, 2 parts of glycerol, 3.5 parts of dimethyl sulfoxide, 2.5 parts of ethylparaben, and 0.8 parts of vitamin C.
[0010] Preferably, the ingredients include 5.5 parts of recombinant human collagen, 6 parts of carbomer, 4 parts of triethanolamine, 4 parts of glycerol, 5 parts of dimethyl sulfoxide, 4 parts of ethylparaben, and 1.2 parts of vitamin C.
[0011] Preferably, 9 parts of recombinant human collagen, 8 parts of carbomer, 7.5 parts of triethanolamine, 6 parts of glycerol, 8 parts of dimethyl sulfoxide, 6 parts of ethylparaben, and 2 parts of vitamin C.
[0012] Compared with the existing technology, when used, the present invention has high biological activity of recombinant human collagen, which can significantly improve the skin repair speed and quickly relieve pain; carbomer and ethylparaben are mainly manifested in anti-inflammatory effects, emulsification stabilization, skin protection, and sustained-release effects; triethanolamine and glycerol have moisturizing and hydrating effects on the skin; the addition of dimethyl sulfoxide can enhance the penetration ability of various drugs, allowing recombinant human collagen to better penetrate into the wound, thereby improving the healing speed of recombinant human collagen, and recombinant human collagen can form a protective layer on the wound surface, acting as a physical barrier, and has the effect of quickly relieving pain and healing oral sores. BRIEF DESCRIPTION OF THE DRAWINGS
[0013] Figure 1 The bar graph shows the cell adhesion rate. DETAILED DESCRIPTION
[0014] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.
[0015] The present invention specifically adopts the following method to prepare recombinant human collagen oral repair dressing, which includes the following steps: Step 1: Mix recombinant human collagen, carbomer, triethanolamine, glycerol, dimethyl sulfoxide, ethylparaben, and vitamin C; Step 2: Then, the purified water is stirred and mixed to prepare the recombinant human collagen oral repair dressing.
[0016] Among them, the recombinant human collagen oral repair dressing includes the following components by weight: 2-18 parts of recombinant human collagen, 2-12 parts of carbomer, 1-10 parts of triethanolamine, 1-8 parts of glycerol, 2-12 parts of dimethyl sulfoxide, 1-10 parts of ethylparaben, and 0.5-3 parts of vitamin C.
[0017] The following are descriptions of different recipes: Example 1 Recombinant human collagen oral repair dressing was prepared by mixing 2 parts of recombinant human collagen, 2 parts of carbomer, 1 part of triethanolamine, 1 part of glycerol, 2 parts of dimethyl sulfoxide, 1 part of ethylparaben, 0.5 part of vitamin C, and then adding purified water.
[0018] Example 2
[0019] Recombinant human collagen oral repair dressing is prepared by mixing 4.5 parts of recombinant human collagen, 4 parts of carbomer, 2.5 parts of triethanolamine, 2 parts of glycerol, 3.5 parts of dimethyl sulfoxide, 2.5 parts of ethylparaben, and 0.8 part of vitamin C with purified water.
[0020] Example 3
[0021] 5.5 parts of recombinant human collagen, 6 parts of carbomer, 4 parts of triethanolamine, 4 parts of glycerol, 5 parts of dimethyl sulfoxide, 4 parts of ethylparaben, and 1.2 parts of vitamin C.
[0022] Example 4
[0023] 9 parts of recombinant human collagen, 8 parts of carbomer, 7.5 parts of triethanolamine, 6 parts of glycerol, 8 parts of dimethyl sulfoxide, 6 parts of ethylparaben, and 2 parts of vitamin C.
[0024] Example 5
[0025] 17 parts of recombinant human collagen, 11 parts of carbomer, 8.5 parts of triethanolamine, 8 parts of glycerol, 11 parts of dimethyl sulfoxide, 9 parts of ethylparaben, and 2.8 parts of vitamin C.
[0026] The following is a verification of the oral repair dressings prepared in Examples 1 to 5: First, in order to verify whether the oral repair dressing is irritating to the skin, mice were selected as experimental subjects and divided into six groups, including five experimental groups, each corresponding to the oral repair dressing prepared in Examples 1 to 5, and the last group of mice was used as a control group; The specific process is as follows: 1. First, select healthy mice of 6-8 weeks old as experimental subjects, with three mice in each group; 2. Remove all the hair on the back of the mouse and make an incision on the skin at the shaved area with a scalpel. It should be noted that the wound only needs to break the skin. A large wound can easily lead to infection in the mouse. 3. Select the oral repair dressings prepared in Examples 1 to 5 and apply them to the wounds of mice. Apply the dressings to each mouse three times a day. 4. Observe the wounds of mice every other day.
[0027] The data from the mouse experiment are shown in the following table (N represents non-healing, X represents beginning to heal, Y represents scarring, and Z represents complete healing):
[0028] The above experiments indicate that, since the mice were all healthy and possessed a certain level of resistance, none of the mice developed infection after six days of observation. However, starting on the second day, a small number of wounds treated with the oral repair dressing began to heal, and by the third day, all wounds had scarred, while the wounds of the experimental group mice had only just begun to heal. This indicates that the oral repair dressings prepared using Examples 1 to 5 promote wound healing without causing skin irritation, and the mice all grew up healthy.
[0029] Second, we took samples from the wounds of patients with oral ulcers and found that the main pathogens in the ulcers were Staphylococcus aureus, Streptococcus mutans, and Porphyromonas gingivalis. 1. Perform colony culture on Staphylococcus aureus, Streptococcus mutans, and Porphyromonas gingivalis, and then divide each into multiple groups; 2. Prepare solution A using the oral repair dressings prepared in Examples 1 to 5; Next, a comparative example was prepared by taking three portions of a commercial oral ulcer spray and using the same method to prepare solution B. 3. The solution A prepared above was used to perform experimental tests on Staphylococcus aureus, Streptococcus mutans, and Porphyromonas gingivalis. The results corresponded to those in Examples 1 to 5. Furthermore, solution B was also tested with Staphylococcus aureus, Streptococcus mutans, and Porphyromonas gingivalis. The results corresponded to comparative example 1, comparative example 2, and comparative example 3, respectively. The specific results are shown in the following table: Table 1:
[0030] It can be seen from Table 1 that the product prepared by the present invention has a significant effect on inhibiting oral bacteria.
[0031] 3. To further verify the effectiveness of the dressing, 6 volunteers suffering from oral ulcers were randomly selected and divided into six groups. The first five groups were given the oral repair dressings prepared in Examples 1 to 5, respectively, and the last group was the control group, which was given a commercially available oral ulcer spray. The results of the patient experiments are shown in the following table:
[0032] After the above treatment for 6 patients, the pain was significantly improved at the beginning of using the oral repair dressing. The pain basically disappeared on the second day. The wound was basically healed on the third day. Most of them stopped taking the medicine on the fourth day. The cure rate was 100%.
[0033] 4. In order to verify the repair effect of the recombinant human collagen oral repair dressing proposed in the invention on human cells, the results of the human fibroblast adhesion test are as follows: 1. Test Purpose This test uses human fibroblasts as the research object. Components of the extracellular matrix are used to coat 96-well plates. After inoculation and cell culture, the adhesion rate of the test sample cells is detected to evaluate the adhesion effect of the test sample on human fibroblasts.
[0034] 2. Test items
[0035] Human fibroblast adhesion assay.
[0036] 3. Test materials
[0037] 3.1 Test System
[0038] Human fibroblasts.
[0039] 3.2 Main Reagents
[0040] Low-glucose DMEM (Solebol), fetal bovine serum (Gibco), PBS (Gibco), MTT (Sigma), DMSO (Sinopharm), and trypsin (Gibco).
[0041] 3.3 Main instruments
[0042] C Incubator (Thermo, 160i), biological safety cabinet (ESC O, LA2-6A1), inverted fluorescence microscope (Keyence BZ-X810), and microplate reader (TeC an, Spark).
[0043] 3.4 Sample Information
[0044] The sample information is as follows: Table 1 Sample information
[0045] 4. Test Method
[0046] 1) Coating culture plates: Coat 96-well plates with 10 μg / mL C ollagen I and store in a 4°C refrigerator overnight; 2) BSA wash: Aspirate the coating solution, add 200 μL of heat-denatured 1% BSA, and incubate the plate at 37°C for 1 hour. 3) Culture medium immersion: Wash the plate twice with serum-free culture medium; 4) Prepare cell suspension: Digest the cells with trypsin to prepare a cell suspension, wash the cells with serum-free medium, and resuspend them; 5) Seeding: Collect cells in the logarithmic growth phase and plate them into pre-treated 96-well plates at a cell density of 5 × 10 4 cells / well; 6) The specific settings of the experimental groups are shown in Table 2: Table 2 Experimental design
[0047] 7) Detection: 6 hours after sample loading, discard the supernatant. Wash non-adherent cells with PBS in the BC, NC, PC, and sample groups. Do not wash in the TC group. 6 hours after sample loading, discard the supernatant. Wash non-adherent cells with PBS. Add 100 μL of culture medium containing 0.5 mg / mL MTT and incubate at 37°C in the dark for 4 hours. After incubation, discard the supernatant, add 50 μL of DMSO to each well, and read the OD value at 490 nm.
[0048] 8) Calculation of cell adhesion rate: Cell adhesion rate = (OD of sample well / OD of total cell well) 100%.
[0049] 5. Test results (see Table 3 and Figure 1 )
[0050] Table 3 Summary of adhesion rate results
[0051] Note: When using the t-test two-tailed test method for statistical analysis, the significance of the NC group compared with the BC group is indicated by #, p-value < 0.05 is indicated by #, and p-value < 0.01 is indicated by ##; the significance of the PC group and the sample group compared with the NC group is indicated by indicates that p-value < 0.05 indicates that , p-value<0.01 is expressed as .
[0052] Combine Figure 1 As shown, the results show: Compared with the BC group, the adhesion rate of human fibroblasts in the NC group was significantly increased (p < 0.01), indicating that the well plate coating was effective.
[0053] Compared with the NC group, the PC group could significantly increase the adhesion rate of human fibroblasts (p < 0.01), indicating that the positive control test was effective.
[0054] Compared with the NC group, the recombinant human collagen sample at concentrations of 0.5 mg / mL and 1.0 mg / mL showed a significant improvement in the adhesion rate of human fibroblasts (p < 0.01).
[0055] 6. Conclusion
[0056] Based on a human fibroblast model, the recombinant human collagen sample significantly increased the adhesion rate of human fibroblasts at both 0.5 mg / mL and 1.0 mg / mL concentrations, with statistically significant differences compared to the NC group (p < 0.01), indicating that the recombinant human collagen sample can promote human fibroblast adhesion. Ultimately, this demonstrates that the oral restorative dressing proposed in this application has a rapid healing effect on oral ulcers.
[0057] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.
Claims
1. A recombinant human collagen oral repair dressing, characterized in that: Includes recombinant human collagen, carbomer, triethanolamine, glycerin, dimethyl sulfoxide, ethylparaben, vitamin C and purified water.
2. The recombinant human collagen oral repair dressing according to claim 1, characterized in that: The preparation method of the recombinant human collagen oral repair dressing is as follows: Step 1: Mix recombinant human collagen, carbomer, triethanolamine, glycerol, dimethyl sulfoxide, ethylparaben, and vitamin C; Step 2: Then, the purified water is stirred and mixed to prepare the recombinant human collagen oral repair dressing.
3. The recombinant human collagen oral repair dressing according to claim 2, characterized in that: The recombinant human collagen oral repair dressing includes the following components by weight: 2-18 parts of recombinant human collagen, 2-12 parts of carbomer, 1-10 parts of triethanolamine, 1-8 parts of glycerol, 2-12 parts of dimethyl sulfoxide, 1-10 parts of ethylparaben, and 0.5-3 parts of vitamin C.
4. The recombinant human collagen oral repair dressing according to claim 3, characterized in that: 2 parts recombinant human collagen, 2 parts carbomer, 1 part triethanolamine, 1 part glycerol, 2 parts dimethyl sulfoxide, 1 part ethylparaben, 0.5 parts vitamin C.
5. The recombinant human collagen oral repair dressing according to claim 3, characterized in that: 4.5 parts of recombinant human collagen, 4 parts of carbomer, 2.5 parts of triethanolamine, 2 parts of glycerol, 3.5 parts of dimethyl sulfoxide, 2.5 parts of ethylparaben, and 0.8 parts of vitamin C.
6. The recombinant human collagen oral repair dressing according to claim 3, characterized in that: 5.5 parts of recombinant human collagen, 6 parts of carbomer, 4 parts of triethanolamine, 4 parts of glycerol, 5 parts of dimethyl sulfoxide, 4 parts of ethylparaben, and 1.2 parts of vitamin C.
7. The recombinant human collagen oral repair dressing according to claim 3, characterized in that: 9 parts of recombinant human collagen, 8 parts of carbomer, 7.5 parts of triethanolamine, 6 parts of glycerol, 8 parts of dimethyl sulfoxide, 6 parts of ethylparaben, and 2 parts of vitamin C.