Micro-droplet characteristic high-throughput detection device and method based on acousto-optic effect

Abstract

The invention provides a micro-droplet characteristic high-flux detection device and method based on an acousto-optic effect, and the method comprises the steps: transmitting a sound wave signal generated by a sound wave generator to a glass slide through the sound wave generator, so that micro-droplets on the glass slide generate periodic vibration deformation; the laser generator sequentially passes through the dichroscope and the objective lens, gathers single-wavelength laser generated by the dichroscope and the objective lens, emits the gathered single-wavelength laser to the micro-droplets on the slide glass, excites echo wall mode laser, generates a plurality of optical signals corresponding to different micro-droplet forms in the micro-droplet deformation process, and transmits the optical signals to the spectrometer sequentially through the objective lens and the dichroscope; the spectrograph detects the characteristics of the micro-droplets according to the optical signals, the micro-droplets are different in form and wavelength and light intensity corresponding to the optical signals are different, and under the action of the same sound wave signals, the micro-droplets are different in characteristics, different in deformation degree and different in wavelength and light intensity change degree corresponding to the optical signals. According to the invention, high-throughput detection of micro-droplet characteristics can be realized, the accuracy and efficiency are high, and the cost is low.

Description

Technical Field

[0001] The present invention belongs to the field of acousto-optic sensing, and in particular relates to a high-throughput detection device and method for micro-droplet characteristics based on acousto-optic effects. Background Art

[0002] The mechanical properties of biofluids (viscosity and surface tension, etc.) can be used as indicators for early diagnosis of diseases, providing rich information for understanding complex physiological and pathological processes. For example, pancreatic cancer patients have abnormal levels of fatty acids and cholesterol in their blood, which leads to changes in blood viscosity. Therefore, extracting changes in mechanical properties from biofluids is essential for improving diagnostic efficiency and expanding biomedical applications. Traditional methods for sensing the mechanical properties of biofluids include microrheology, atomic force microscopy, Brillouin spectroscopy, optical tweezers, etc. However, due to their low measurement efficiency, they are unable to meet the needs of high-throughput sensing. Summary of the Invention

[0003] The present invention provides a high-throughput detection device and method for micro-droplet characteristics based on acousto-optic effects, so as to solve the problem of low efficiency in the current detection of micro-droplet characteristics (viscosity coefficient).

[0004] According to a first aspect of an embodiment of the present invention, a high-throughput detection device for micro-droplet characteristics based on acousto-optic effects is provided, comprising a laser generator, an acoustic wave generator, a dichroic mirror, an objective lens, a carrier plate, and a spectrometer. The acoustic wave generator transmits an acoustic wave signal generated by the acoustic wave generator to the carrier plate, causing the micro-droplets on the carrier plate to undergo periodic vibration and deformation. The laser generator converges the single-wavelength laser light generated by the laser generator through the dichroic mirror and the objective lens, and then directs the laser light toward the micro-droplets on the carrier plate, thereby stimulating whispering gallery mode laser light. During the deformation of the micro-droplets, multiple optical signals corresponding to different micro-droplet morphologies are generated. The optical signals are transmitted to the spectrometer through the objective lens and the dichroic mirror. The spectrometer detects the characteristics of the micro-droplets based on the optical signals. Different morphologies of the same micro-droplet correspond to different wavelengths and intensities of the corresponding optical signals. Moreover, under the action of the same acoustic wave signal, the characteristics of the micro-droplets are different, the deformation degree of the micro-droplets is different, and the wavelength and intensity of the corresponding optical signals vary to different degrees.

[0005] Optionally, the microdroplets are blood microdroplets and are arranged in an array on a glass slide; high-throughput detection of the viscosity coefficient is performed based on acousto-optic effect;

[0006] The system also includes a beam splitter, a camera, a first convex lens and a second convex lens. The laser generator converges the single-wavelength laser light generated by the laser generator through the dichroic mirror and the objective lens in sequence, and then emits the convergent laser light toward the micro-droplet on the carrier. The whispering gallery mode laser signal output by the micro-droplet is transmitted to the beam splitter through the objective lens and the dichroic mirror in sequence, thereby transmitting multiple optical signals corresponding to different micro-droplet morphologies to the beam splitter. The beam splitter transmits the optical signal to the spectrometer through the first convex lens. According to the incident position of the single-wavelength laser light on the micro-droplet displayed on the camera, it is determined whether the micro-droplet has moved into position.

[0007] Optionally, the optical filter is further included between the dichroic mirror and the beam splitter, and the optical filter is used to filter out the single-wavelength laser generated by the laser generator.

[0008] Optionally, the apparatus further includes a two-dimensional electric translation stage, the carrier plate is disposed on the two-dimensional electric translation stage, and the two-dimensional electric translation stage drives the carrier plate to move on a two-dimensional plane, so that the single-wavelength laser continuously scans different blood micro-droplets in the blood micro-droplet array on the carrier plate;

[0009] The device also includes a printer. The blood liquid to be tested is injected into the ink cartridge of the printer, and the blood micro-droplets are printed on the carrier sheet by using the print head of the printer.

[0010] Optionally, the blood microdroplets are made by mixing a gain medium and blood; and a super-hydrophobic material layer is coated on the carrier plate, so that nearly spherical blood microdroplets are formed on the super-hydrophobic material layer.

[0011] According to a second aspect of an embodiment of the present invention, a high-throughput detection method for micro-droplet characteristics based on acousto-optic interaction is provided, comprising:

[0012] Step S100: transmitting an acoustic wave signal to a carrier plate to cause the microdroplet on the carrier plate to periodically vibrate and deform. Simultaneously, a single-wavelength laser is focused and directed toward the microdroplet on the carrier plate to excite whispering gallery mode lasers. During the microdroplet deformation process, multiple whispering gallery mode laser signals corresponding to different droplet morphologies are output from the microdroplet.

[0013] Step S200: Detect the viscosity coefficient of the blood microdroplet based on the optical signal, wherein the same microdroplet has different shapes, corresponding to different wavelengths and light intensities of the corresponding optical signal, and under the action of the same acoustic wave signal, the viscosity coefficient of the microdroplet is different, the deformation degree of the microdroplet is different, and the wavelength and light intensity of the corresponding optical signal change differently.

[0014] Optionally, an acoustic wave generator is used to generate acoustic waves to cause the micro-droplets on the glass slide to vibrate and deform periodically. The step S200 specifically includes performing the following steps:

[0015] Step S210: For each of the obtained time domain spectra corresponding to the vibration period of the blood microdroplet, sequentially calculate the correlation between the first obtained time domain spectrum and each of the subsequent time domain spectra, as well as the standard deviation of the correlation curve;

[0016] Step S220: Calculate the blood viscosity coefficient according to the calibration curve.

[0017] Optionally, step S220 specifically includes:

[0018] Step S221: using mixtures of glycerol and water in different volume ratios to produce micro-droplets with different viscosity coefficients on the surface of a carrier plate coated with a super-hydrophobic material;

[0019] Step S222: Using an acoustic wave generator to generate acoustic waves, the micro-droplets on the carrier plate vibrate and deform. The micro-droplets with different viscosity coefficients vibrate and deform to different degrees, corresponding to different degrees of change in the output echo wave mode laser signal.

[0020] Step S223: The spectrometer collects the laser signal spectra generated by the micro-droplets with different viscosity coefficients in real time, and calculates different correlations and standard deviations of the correlation curves based on the time-domain spectra of the micro-droplets with different viscosity coefficients; and calibrates a calibration curve of the viscosity coefficient and the standard deviation of the spectrum correlation curve.

[0021] Optionally, the blood microdroplets on the carrier plate are arranged in an array, and after step S200, the method further includes: moving the carrier plate by the two-dimensional electric translation stage so that the single-wavelength laser continuously scans different blood microdroplets in the blood microdroplet array on the carrier plate, thereby realizing high-throughput detection of the viscosity coefficient of the blood microdroplets; and returning to execute step S100.

[0022] The beneficial effects of the present invention are:

[0023] 1. The present invention uses an acoustic wave generator to generate an acoustic wave signal, which is transmitted to the microdroplets via a carrier plate, causing the microdroplets to undergo periodic vibration and deformation. A single-wavelength laser generated by a laser generator converges and irradiates the microdroplets on the carrier plate, causing them to output whispering gallery-mode laser light. The output laser signal changes with the droplet morphology. The optical signal is transmitted to a spectrometer via an objective lens and a dichroic mirror. Under the action of the same acoustic wave signal, the microdroplets deform to varying degrees due to differences in their characteristics, corresponding to varying degrees of change in the optical signal. Based on the real-time optical signal collected by the spectrometer, high-throughput detection of microdroplet characteristics is achieved. Furthermore, the present invention is simple to construct and low-cost.

[0024] 2. The present invention is provided with an objective lens, a beam splitter, and a camera to perform microscopic imaging of blood microdroplets; whether the microdroplets have moved into position is determined based on the incident position of the single-wavelength laser on the blood microdroplets displayed on the camera;

[0025] 3. The present invention sets a filter to filter out the single-wavelength laser generated by the laser generator, thereby eliminating the influence of the single-wavelength laser on the overall time domain spectrum, thereby ensuring detection accuracy;

[0026] 4. The present invention arranges blood microdroplets in an array on a slide, and then utilizes a two-dimensional motorized translation stage to drive the slide to achieve continuous scanning of the laser generator across the slide. This allows for automatic switching between different microdroplets in the array for viscosity coefficient detection, thereby improving detection efficiency and enabling high-throughput detection of microdroplet characteristics.

[0027] 5. The present invention coats a super-hydrophobic material layer on a carrier wafer and forms blood microdroplets on the super-hydrophobic material layer, thereby ensuring that the microdroplets form a nearly spherical structure. A gain medium is added to the blood microdroplets to enable them to support whispering gallery mode laser output under laser pumping.

[0028] The blood microdroplets support whispering gallery mode laser output, and their output optical signals vary with the droplet morphology. An acoustic wave generator generates an acoustic wave signal and transmits it to the carrier chip, causing the microdroplets on the carrier chip to produce periodic vibration and deformation. The optical signal emitted by the microdroplets is transmitted to the spectrometer via the objective lens and dichroic mirror. Under the action of the same acoustic wave signal, the blood microdroplets deform to varying degrees due to differences in their viscosity coefficients, resulting in varying degrees of change in the corresponding optical signal. The viscosity coefficient of the blood is then detected in high throughput mode by calculating the correlation between the real-time optical signals of different droplets in the droplet array collected by the spectrometer.

[0029] 6. The present invention uses a spectrometer to collect the time-domain spectra of blood microdroplets under acoustic wave loading. First, the correlation between the first time-domain spectrum obtained and each subsequent time-domain spectrum is calculated in sequence. Then, the standard deviation of the curve is determined based on the correlation curve. Then, the viscosity coefficient of the microdroplets is determined based on the standard deviation of the curve and the calibration curve. The method for determining the viscosity coefficient is very simple and can further improve the detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 1 is a schematic structural diagram of an embodiment of a high-throughput detection device for micro-droplet characteristics based on acousto-optic effects of the present invention;

[0031] Figure 2 1 is a schematic structural diagram of an embodiment of the sound wave generator of the present invention;

[0032] Figure 32 is a schematic structural diagram of another embodiment of the high-throughput detection device for micro-droplet characteristics based on acousto-optic effects of the present invention;

[0033] Figure 4 This is a schematic diagram of the process of making the blood micro-droplet array of the present invention;

[0034] Figure 5 Schematic diagram of the relationship between the correlation curve and the standard deviation of the correlation curve and the blood viscosity coefficient of patients with hyperlipidemia;

[0035] Figure 6 It is a schematic diagram showing the relationship between the standard deviation of the correlation curve and the viscosity coefficient calibration curve, as well as the viscosity coefficient and the severity of hyperlipidemia;

[0036] Figure 7 This is a flow chart of an embodiment of the high-throughput detection method for micro-droplet characteristics based on acousto-optic effects of the present invention. DETAILED DESCRIPTION

[0037] In order to enable those skilled in the art to better understand the technical solutions in the embodiments of the present invention and to make the above-mentioned purposes, features and advantages of the embodiments of the present invention more obvious and easy to understand, the technical solutions in the embodiments of the present invention are further described in detail below with reference to the accompanying drawings.

[0038] In the description of the present invention, unless otherwise specified and limited, it should be noted that the term "connection" should be understood in a broad sense. For example, it can be a mechanical connection or an electrical connection, or it can be the internal connection between two elements. It can be a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meaning of the above terms can be understood according to the specific circumstances.

[0039] See also Figure 1, which is a structural schematic diagram of an embodiment of a high-throughput detection device for micro-droplet characteristics based on acousto-optic effects of the present invention. The device may include a laser generator 1, an acoustic wave generator, a dichroic mirror 2, an objective lens 3, a carrier plate 5 and a spectrometer 10. The acoustic wave generator transmits the acoustic wave signal it generates to the carrier plate 5 to cause the microdroplets 4 on the carrier plate 5 to undergo periodic vibration and deformation. The laser generator 1 sequentially passes through the dichroic mirror 2 and the objective lens 3 to converge the single-wavelength laser light it generates and then emits it to the microdroplets 4 on the carrier plate 5, stimulating whispering gallery mode laser light. In the process of microdroplet deformation, multiple optical signals corresponding to different microdroplet morphologies are generated. The optical signals are sequentially transmitted to the spectrometer 10 through the objective lens 3 and the dichroic mirror 2. The spectrometer 10 detects the characteristics of the microdroplets 4 based on the optical signals. Different morphologies of the same microdroplet 4 correspond to different wavelengths and intensities of the corresponding optical signals. Moreover, under the action of the same acoustic wave signal, the characteristics of the microdroplets 4 are different, the deformation degree of the microdroplets 4 is different, and the wavelength and intensity of the corresponding optical signals vary to different degrees.

[0040] In this embodiment, the laser generator 1 can be connected to the microdroplet 4 in sequence via the dichroic mirror 2 and the objective lens 3, and the microdroplet 4 can be connected to the spectrometer 10 in sequence via the objective lens 3 and the dichroic mirror 2. The laser generator 1 can be a pulsed laser, and the single-wavelength laser it produces can be a high-repetition-rate pulsed laser with a wavelength of 532nm, a repetition rate of 500Hz, and a pulse width of ≤10ns. By giving the single-wavelength laser a high repetition-rate characteristic, the present invention can increase the number of optical signals corresponding to the microdroplet morphology obtained per unit time, reduce the spectrum acquisition time of a single microdroplet, and thus significantly reduce the droplet scanning time, thereby improving detection efficiency and ensuring detection accuracy. In addition, the narrow pulse width of the single-wavelength laser can give it a higher peak power, making it easier to reach the laser threshold. The acoustic wave signal generated by the acoustic wave generator can be a sinusoidal signal. The carrier wave 5 can be coated with a super-hydrophobic material layer so that the microdroplet 4 is formed on this super-hydrophobic material layer, thereby ensuring that the microdroplet forms a nearly spherical structure. The microdroplet may be blood, and the characteristic thereof may be the viscosity of blood.

[0041] The present invention is based on the acousto-optic effect, causing the microdroplets on the carrier chip to undergo periodic vibration and deformation under the action of sound waves. The present invention also converges a single-wavelength laser and shoots it at the microdroplets. In this way, the time-domain spectrum collected by the spectrometer can be composed of multiple optical signals corresponding to different microdroplet morphologies. Different microdroplet morphologies correspond to different wavelengths and light intensities of the corresponding optical signals.

[0042] Combine Figure 2As shown, the acoustic wave generator may include a signal generator 13 and a piezoelectric transducer 12 connected to each other. The piezoelectric transducer 12 is in contact with the carrier 5. The piezoelectric transducer 12 generates an acoustic wave signal based on the electrical signal generated by the signal generator 13 and transmits the acoustic wave signal to the carrier 5. The electrical signal may be a sinusoidal wave driving signal, so that the micro-droplets vibrate periodically under the action of the generated sinusoidal acoustic wave signal.

[0043] In addition, the microdroplets are blood microdroplets arranged in an array on a glass slide; high-throughput detection of blood viscosity is performed based on acousto-optic effects; the microdroplets can be made from a mixture of a gain medium and blood, wherein the gain medium can be a rhodamine fuel; the carrier is coated with a superhydrophobic material layer, so that nearly spherical blood microdroplets are formed on the superhydrophobic material layer. The present invention adds a gain medium to the blood microdroplets, enabling them to support whispering gallery mode laser output under laser pumping; the present invention coats the carrier with a superhydrophobic material layer, and forms the microdroplets on the superhydrophobic material layer, thereby ensuring that the microdroplets form a nearly spherical structure. The blood microdroplets support whispering gallery mode laser output, and their output light signals vary with the droplet morphology. The acoustic wave generator generates an acoustic wave signal and transmits it to the carrier chip, causing the microdroplets on the carrier chip to produce periodic vibration and deformation. The light signal emitted by the microdroplets is transmitted to the spectrometer by the objective lens and the dichroic mirror. Under the action of the same acoustic wave signal, the blood microdroplets deform to different degrees due to differences in their viscosity coefficients, resulting in different degrees of change in the corresponding light signals. The viscosity coefficient of the blood is detected in high throughput mode based on correlation calculation of the real-time light signals of different droplets in the droplet array collected by the spectrometer.

[0044] As can be seen from the above embodiments, the present invention uses an acoustic wave generator to generate an acoustic wave signal, which is transmitted to the microdroplets via a carrier plate, causing the microdroplets to undergo periodic vibration and deformation. A single-wavelength laser generated by a laser generator converges and irradiates the microdroplets on the carrier plate, causing them to output whispering gallery-mode laser light, with the output laser signal varying with the droplet morphology. The optical signal is transmitted to a spectrometer via an objective lens and a dichroic mirror. Under the action of the same acoustic wave signal, differences in the microdroplet's characteristics lead to varying degrees of deformation, corresponding to varying degrees of change in the optical signal. Based on the real-time optical signal collected by the spectrometer, high-throughput detection of microdroplet characteristics is achieved. Furthermore, the present invention is simple to construct and low-cost.

[0045] See also Figure 3 , which is a structural schematic diagram of another embodiment of the high-throughput detection device for micro-droplet characteristics based on acousto-optic effects of the present invention. Figure 3 and Figure 1The difference between the high-throughput detection device for micro-droplet characteristics shown is that it can also include a beam splitter 8, a camera 11, a first convex lens 9, and a second convex lens 12. The laser generator 1 sequentially passes through the dichroic mirror 2 and the objective lens 3 to converge the single-wavelength laser light it generates and then emit it to the micro-droplet 4 on the carrier 5; the echo gallery mode laser signal output by the micro-droplet 4 is sequentially transmitted through the objective lens 3 and the dichroic mirror 2 to the beam splitter 8, thereby transmitting multiple light signals corresponding to different micro-droplet morphologies to the beam splitter 8, and the beam splitter 8 transmits the light signal to the spectrometer 10 through the first convex lens 9; based on the incident position of the single-wavelength laser on the micro-droplet displayed in the camera 11, it is determined whether the micro-droplet has moved into position (for example, it moves into position when the incident position is at the center of the micro-droplet). The present invention is provided with an objective lens, a beam splitter, and a camera to perform microscopic imaging of blood micro-droplets; based on the incident position of the single-wavelength laser on the blood micro-droplet displayed in the camera, it is determined whether the micro-droplet has moved into position.

[0046] Figure 3 and Figure 1 The high-throughput detection device for droplet characteristics shown above differs in that it also includes a filter 7 positioned between the dichroic mirror 2 and the beam splitter 8 to filter out the single-wavelength laser light generated by the laser generator. By using a filter to filter out the single-wavelength laser light generated by the laser generator, the present invention eliminates the impact of the single-wavelength laser light on the overall time-domain spectrum, thereby ensuring detection accuracy.

[0047] Figure 3 and Figure 1 The difference of the high-throughput detection device for micro-droplet characteristics shown is that it combines Figure 4 As shown, the microdroplets 4 on the slide 5 are arranged in an array. The device may also include a two-dimensional motorized translation stage 6, on which the slide 5 is mounted. The two-dimensional motorized translation stage 6 drives the slide 5 to move in a two-dimensional plane, allowing the single-wavelength laser to continuously scan different blood droplets in the blood droplet array on the slide 5. The present invention arranges blood droplets in an array on the slide, and then uses the two-dimensional motorized translation stage to drive the slide to move, enabling the laser generator to continuously scan the slide. This allows for automatic switching between different droplets in the array for viscosity coefficient detection, thereby improving detection efficiency and enabling high-throughput detection of droplet characteristics. The device may also include a processor connected to the camera 11 and the two-dimensional motorized translation stage 6, respectively. The processor detects the incident position of the single-wavelength laser on the droplet, as displayed by the camera, and controls the two-dimensional motorized translation stage 6 based on the incident position, causing the two-dimensional motorized translation stage 6 to move the droplets on the slide 5 into position.

[0048] In addition, combined Figure 4 As shown, Figure 3and Figure 1 The difference between the high-throughput detection device for micro-droplet characteristics shown in the figure is that it can also include a printer 15. The blood liquid 14 to be tested is injected into the ink cartridge of the printer 15, and the print head 16 of the printer 15 prints the blood micro-droplets 4 on the carrier plate 5. Figure 4 12 refers to a piezoelectric transducer. The present invention uses a printer to produce micro-droplet arrays in large quantities at low cost. Figure 5 and Figure 6 As shown, the larger the amplitude of the microdroplet, the lower the viscosity; the larger the standard deviation of the correlation curve, the lower the viscosity; and the lower the viscosity, the lower the severity of hyperlipidemia.

[0049] As can be seen from the above embodiments, the present invention uses an acoustic wave generator to generate an acoustic wave signal, which is transmitted to the microdroplets via a carrier plate, causing the microdroplets to undergo periodic vibration and deformation. A single-wavelength laser generated by a laser generator converges and irradiates the microdroplets on the carrier plate, causing them to output whispering gallery-mode laser light, with the output laser signal varying with the droplet morphology. The optical signal is transmitted to a spectrometer via an objective lens and a dichroic mirror. Under the action of the same acoustic wave signal, differences in the microdroplet's characteristics lead to varying degrees of deformation, corresponding to varying degrees of change in the optical signal. Based on the real-time optical signal collected by the spectrometer, high-throughput detection of microdroplet characteristics is achieved. Furthermore, the present invention is simple to construct and low-cost.

[0050] See also Figure 7 , is a flow chart of an embodiment of a high-throughput detection method for micro-droplet characteristics based on acousto-optic effects of the present invention. The high-throughput detection method for micro-droplet characteristics based on acousto-optic effects may include:

[0051] Step S100: transmitting an acoustic wave signal to a carrier plate to cause the microdroplet on the carrier plate to periodically vibrate and deform. Simultaneously, a single-wavelength laser is focused and directed toward the microdroplet on the carrier plate to excite whispering gallery mode lasers. During the microdroplet deformation process, multiple whispering gallery mode laser signals corresponding to different microdroplet morphologies are output (e.g., reflected) from the microdroplet.

[0052] Step S200: Detect the viscosity coefficient of the blood microdroplet based on the optical signal, wherein the same microdroplet has different shapes, corresponding to different wavelengths and light intensities of the corresponding optical signal, and under the action of the same acoustic wave signal, the viscosity coefficient of the microdroplet is different, the deformation degree of the microdroplet is different, and the wavelength and light intensity of the corresponding optical signal change differently.

[0053] In this embodiment, an acoustic wave generator is used to generate acoustic waves to cause the micro-droplets on the glass slide to vibrate and deform periodically. The step S200 may specifically include performing the following steps:

[0054] Step S210: For each of the obtained time domain spectra corresponding to the vibration period of the blood microdroplet, sequentially calculate the correlation between the first obtained time domain spectrum and each of the subsequent time domain spectra;

[0055] Step S220: Calculate the blood viscosity coefficient according to the calibration curve.

[0056] The step S220 may specifically include:

[0057] Step S221: using mixtures of glycerol and water in different volume ratios to produce micro-droplets with different viscosity coefficients on the surface of a carrier plate coated with a super-hydrophobic material;

[0058] Step S222: Using an acoustic wave generator to generate acoustic waves, the micro-droplets on the carrier plate vibrate and deform. The micro-droplets with different viscosity coefficients vibrate and deform to different degrees, corresponding to different degrees of change in the output echo wave mode laser signal.

[0059] Step S223: The spectrometer collects the laser signal spectra generated by the micro-droplets with different viscosity coefficients in real time, and calculates different correlations based on the time-domain spectra of each micro-droplet with different viscosity coefficient; and calibrates a calibration curve of viscosity coefficient and spectrum correlation.

[0060] The microdroplets can be made by mixing a gain medium and blood. The present invention adds a gain medium to the blood microdroplets, so that they support whispering gallery mode laser output under the pumping of a laser generator; the present invention coats a superhydrophobic material layer on a carrier plate, and forms microdroplets on the superhydrophobic material layer, thereby ensuring that the microdroplets form a nearly spherical structure. The blood microdroplets support whispering gallery mode laser output, and their output optical signals vary with the droplet morphology; the acoustic wave generator generates an acoustic wave signal and transmits it to the carrier plate, so that the microdroplets on the carrier plate produce periodic vibration deformation; the optical signal emitted by the microdroplets is transmitted to the spectrometer by the objective lens and dichroic mirror; under the action of the same acoustic wave signal, due to the difference in the viscosity coefficient of the blood microdroplets, the blood microdroplets deform to different degrees, corresponding to different degrees of change in the corresponding optical signal; based on the correlation calculation of the real-time optical signals of different droplets in the droplet array collected by the spectrometer, the viscosity coefficient of blood is detected in high throughput.

[0061] The present invention uses a spectrometer to collect time-domain spectra of blood microdroplets under acoustic wave loading. First, the correlation between the first time-domain spectrum obtained and each subsequent time-domain spectrum is calculated in sequence. Then, the curve standard deviation is determined based on the correlation curve. Then, the viscosity coefficient of the microdroplet is determined based on the curve standard deviation and the calibration curve. The method for determining the viscosity coefficient is very simple and can further improve detection efficiency.

[0062] The blood microdroplets on the carrier plate are arranged in an array. After step S200, the method may further include: moving the carrier plate by the two-dimensional electric translation stage so that the single-wavelength laser continuously scans different blood microdroplets in the blood microdroplet array on the carrier plate to achieve high-throughput detection of the viscosity coefficient of the blood microdroplets; and returning to step S100.

[0063] As can be seen from the above embodiments, the present invention uses an acoustic wave generator to generate an acoustic wave signal, which is transmitted to the microdroplets via a carrier plate, causing the microdroplets to undergo periodic vibration and deformation. A single-wavelength laser generated by a laser generator converges and irradiates the microdroplets on the carrier plate, causing them to output whispering gallery-mode laser light, with the output laser signal varying with the droplet morphology. The optical signal is transmitted to a spectrometer via an objective lens and a dichroic mirror. Under the action of the same acoustic wave signal, differences in the microdroplet's characteristics lead to varying degrees of deformation, corresponding to varying degrees of change in the optical signal. Based on the real-time optical signal collected by the spectrometer, high-throughput detection of microdroplet characteristics is achieved. Furthermore, the present invention is simple to construct and low-cost.

[0064] Other embodiments of the present invention will readily occur to those skilled in the art after considering the specification and practicing the invention disclosed herein. This application is intended to cover any variations, uses, or adaptations of the present invention that follow the general principles of the invention and include common knowledge or customary techniques in the art not disclosed herein. The description and examples are to be considered as exemplary only, with the true scope and spirit of the invention being indicated by the following claims.

[0065] It will be appreciated that the present invention is not limited to the precise construction that has been described above and shown in the accompanying drawings, and that various modifications and variations can be made without departing from its scope, which is governed solely by the appended claims.

Claims

1. A high-throughput detection device for micro-droplet characteristics based on acousto-optic effects, characterized in that: The invention comprises a laser generator, an acoustic wave generator, a dichroic mirror, an objective lens, a carrier plate and a spectrometer. The acoustic wave generator transmits the acoustic wave signal generated by the acoustic wave generator to the carrier plate, so that the micro-droplets on the carrier plate undergo periodic vibration and deformation. The laser generator converges the single-wavelength laser light generated by the laser generator through the dichroic mirror and the objective lens in sequence, and then emits the laser light to the micro-droplets on the carrier plate, thereby stimulating the whispering gallery mode laser light. In addition, a plurality of optical signals corresponding to different micro-droplet morphologies are generated during the deformation of the micro-droplets. The optical signals are transmitted to the spectrometer through the objective lens and the dichroic mirror in sequence. The spectrometer detects the characteristics of the micro-droplets based on the optical signals. Different morphologies of the same micro-droplet correspond to different wavelengths and intensities of the corresponding optical signals. Moreover, under the action of the same acoustic wave signal, the characteristics of the micro-droplets are different, the deformation degrees of the micro-droplets are different, and the wavelengths and intensities of the corresponding optical signals vary to different degrees.

2. The high-throughput detection device for micro-droplet characteristics based on acousto-optic effect according to claim 1, characterized in that: The microdroplets are blood droplets arranged in an array on a glass slide; high-throughput detection of viscosity coefficient is performed based on acousto-optic effects; The system also includes a beam splitter, a camera, a first convex lens and a second convex lens. The laser generator converges the single-wavelength laser light generated by the laser generator through the dichroic mirror and the objective lens in sequence, and then emits the convergent laser light toward the micro-droplet on the carrier. The whispering gallery mode laser signal output by the micro-droplet is transmitted to the beam splitter through the objective lens and the dichroic mirror in sequence, thereby transmitting multiple optical signals corresponding to different micro-droplet morphologies to the beam splitter. The beam splitter transmits the optical signal to the spectrometer through the first convex lens. According to the incident position of the single-wavelength laser light on the micro-droplet displayed on the camera, it is determined whether the micro-droplet has moved into position.

3. The high-throughput detection device for micro-droplet characteristics based on acousto-optic effect according to claim 1 or 2, characterized in that: The invention also comprises an optical filter arranged between the dichroic mirror and the optical beam splitter, wherein the optical filter is used for filtering out the single-wavelength laser generated by the laser generator.

4. The high-throughput detection device for micro-droplet characteristics based on acousto-optic effect according to claim 3, characterized in that: The invention also includes a two-dimensional electric translation stage, the carrier plate is arranged on the two-dimensional electric translation stage, and the two-dimensional electric translation stage drives the carrier plate to move on a two-dimensional plane, so that the single-wavelength laser continuously scans different blood micro-droplets in the blood micro-droplet array on the carrier plate; The device also includes a printer. The blood liquid to be tested is injected into the ink cartridge of the printer, and the blood micro-droplets are printed on the carrier sheet by using the print head of the printer.

5. The high-throughput detection device for micro-droplet characteristics based on acousto-optic effect according to claim 1, characterized in that: The blood micro-droplets are made by mixing a gain medium and blood; a super-hydrophobic material layer is coated on the carrier plate, so that blood micro-droplets close to spherical shapes are formed on the super-hydrophobic material layer.

6. A high-throughput detection method for micro-droplet characteristics based on acousto-optic interaction, characterized in that: include: Step S100: transmitting an acoustic wave signal to a carrier chip to cause the blood microdroplets on the carrier chip to vibrate and deform periodically. Simultaneously, a single-wavelength laser is focused and directed toward the microdroplets on the carrier chip to stimulate whispering gallery mode lasers. During the deformation of the microdroplets, multiple whispering gallery mode laser signals corresponding to different droplet morphologies are emitted from the microdroplets. Step S200: Detect the viscosity coefficient of the blood microdroplet based on the optical signal, wherein the same microdroplet has different shapes, corresponding to different wavelengths and light intensities of the corresponding optical signal, and under the action of the same acoustic wave signal, the viscosity coefficient of the microdroplet is different, the deformation degree of the microdroplet is different, and the wavelength and light intensity of the corresponding optical signal change differently.

7. The high-throughput detection method for micro-droplet characteristics based on acousto-optic interaction according to claim 6, characterized in that: The acoustic wave generator is used to generate acoustic waves to cause the micro-droplets on the glass slide to vibrate and deform periodically. The step S200 specifically includes performing the following steps: Step S210: For each of the obtained time domain spectra corresponding to the vibration period of the blood microdroplet, sequentially calculate the correlation between the first obtained time domain spectrum and each of the subsequent time domain spectra, as well as the standard deviation of the correlation curve; Step S220: Calculate the blood viscosity coefficient according to the calibration curve.

8. The high-throughput detection method for micro-droplet characteristics based on acousto-optic interaction according to claim 7, characterized in that: The step S220 specifically includes: Step S221: using mixtures of glycerol and water in different volume ratios to produce micro-droplets with different viscosity coefficients on the surface of a carrier plate coated with a super-hydrophobic material; Step S222: Using an acoustic wave generator to generate acoustic waves, the micro-droplets on the carrier plate vibrate and deform. The micro-droplets with different viscosity coefficients vibrate and deform to different degrees, corresponding to different degrees of change in the output echo wave mode laser signal. Step S223: The spectrometer collects the laser signal spectra generated by the micro-droplets with different viscosity coefficients in real time, and calculates different correlations and standard deviations of the correlation curves based on the time-domain spectra of the micro-droplets with different viscosity coefficients; and calibrates a calibration curve of the viscosity coefficient and the standard deviation of the spectrum correlation curve.

9. The high-throughput detection method for micro-droplet characteristics based on acousto-optic interaction according to claim 6, characterized in that: The blood microdroplets on the carrier plate are arranged in an array. After step S200, the method further includes: moving the carrier plate by the two-dimensional electric translation stage so that the single-wavelength laser continuously scans different blood microdroplets in the blood microdroplet array on the carrier plate to achieve high-throughput detection of the viscosity coefficient of the blood microdroplets; and returning to step S100.

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