Oil-control acne-removal composition and application thereof

Abstract

The invention relates to the field of cosmetics, in particular to an oil-controlling and acne-removing composition and application thereof. The black tea fermentation liquor, the olive leaf extract and the silybum marianum extract are compounded and have a synergistic effect after being combined, a stronger oil control network is formed, and the oil control effect is greatly improved compared with that when a single component is used; a multi-channel and multi-target acne removal path is formed, and the acne removal effect is multi-dimensionally improved compared with that when a single component is used; the skin barrier function can be further enhanced, the water loss is reduced, and the acne skin damaged skin can be recovered more quickly. The black tea fermentation liquor, the olive leaf extract and the silybum marianum extract are all natural plant extracts, are high in safety and are suitable for sensitive skin. After the three components are compounded, the safety is high, the oil-controlling and acne-removing effects of the oil-controlling and acne-removing composition are remarkably improved through the synergistic effect, and the acne problem can be more comprehensively solved.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and in particular to an oil-controlling and acne-removing composition and application thereof. Background Art

[0002] In recent years, with increasing environmental pollution, growing stress, and widespread unhealthy skincare habits, acne (especially among those with oily skin) has become a global skin health problem. Acne is closely related to excessive sebum secretion, abnormal keratinization of hair follicles, proliferation of Propionibacterium acnes, and inflammatory responses.

[0003] Currently, there are a wide variety of oil-control and acne-removing products on the market. Some powerful acne-removing products primarily utilize salicylic acid and its derivatives, sometimes combined with ingredients like papain. These products achieve oil control by intensely cleansing pores. However, these products suffer from weak long-term oil control or are highly irritating, which can lead to dry skin, fine lines, and other issues. For example, while some products may clean pores in the short term, they cannot effectively control oil secretion over the long term, leading to recurring acne. Meanwhile, some highly effective products can cause discomfort such as redness and itching, which can exacerbate skin problems, especially for sensitive skin. Therefore, developing safe, multi-functional, and low-irritation natural ingredient combinations has become a research hotspot. Summary of the Invention

[0004] The purpose of the present invention is to overcome the shortcomings of the prior art and provide an oil-control and acne-removing composition with triple targeted oil-control and acne-removing effects.

[0005] To achieve the above object, the technical solution adopted by the present invention is:

[0006] In a first aspect, the present invention provides an oil-controlling and acne-removing composition comprising the following components in parts by weight: 1-10 parts of black tea fermentation broth, 0.5-20 parts of olive leaf extract, and 0.1-0.8 parts of milk thistle extract.

[0007] The primary active ingredient in black tea fermentation broth is tea polyphenols, a class of polyhydroxyphenolic compounds extracted from tea leaves. These compounds primarily include catechins, flavonoids, and anthocyanins. By inhibiting the activity of 5α-reductase in the sebaceous glands, tea polyphenols can significantly reduce sebum secretion, reduce skin shine, and exert a significant oil-control effect. Furthermore, tea polyphenols effectively alleviate the redness and inflammation caused by acne by inhibiting the release of inflammatory factors (such as IL-6 and TNF-α) and the growth of Propionibacterium acnes. Furthermore, tea polyphenols can protect skin cells from environmental pollution and endogenous aging factors by scavenging free radicals and regulating pathways related to oxidative stress. Olive leaf extract, a natural ingredient extracted from the leaves of the olive tree (Olea europaea), a plant of the Oleaceae family, is a byproduct of olive oil processing and is rich in polyphenolic compounds. Its core active ingredients include oleuropein and hydroxytyrosol. Olive leaf extract can regulate sebum secretion, inhibiting excessive oil production and exerting an inhibitory effect on Propionibacterium acnes. In the early stages of inflammation, its active ingredients (such as oleuropein) regulate the NF-κB signaling pathway, inhibiting the release of pro-inflammatory factors (such as IL-6 and IL-8), stimulating macrophage activity, and enhancing their phagocytic and bactericidal abilities. In the later stages of inflammation, by inhibiting the NF-κB signaling pathway, it reduces the release of inflammatory factors while promoting cell proliferation and collagen deposition, accelerating tissue repair. These effects of olive leaf extract enable it to effectively relieve skin symptoms such as redness, swelling, and itching, and possess excellent anti-inflammatory, oil-control, antibacterial, and repair properties. Milk thistle extract is a natural ingredient extracted from milk thistle seeds. Its main active substance is silymarin, which is primarily composed of flavonoid lignans such as silybin, isosilybin, silybintin, and silybinin, as well as unknown oxidized polyphenols. Silybin has the highest biological activity, accounting for approximately 50% to 70% of silymarin. Its active ingredient, silymarin, possesses powerful antioxidant properties, effectively scavenging free radicals and reducing lipid peroxidation, thereby protecting the skin from oxidative damage and delaying skin aging. Furthermore, it promotes collagen production in the dermis, restoring skin's natural health, helping to reduce transepidermal water loss and improving skin barrier function.

[0008] After black tea fermentation liquid and olive leaf extract are applied to cosmetics, they are easily oxidized and inactivated under illumination and high temperature conditions, and their active ingredients are easily degraded, which then causes their oil control and anti-acne effects to decline. Milk thistle extract is widely used in medicines, and its application in cosmetics mainly plays an antioxidant and anti-wrinkle effect, but the research in oil control, anti-acne is relatively scarce. The present invention has found through a large number of studies that after black tea fermentation liquid, olive leaf extract and milk thistle extract are compounded, black tea fermentation liquid is except having oil control effect, also has powerful antioxidant efficacy, olive leaf extract anti-inflammatory and antibacterial effects are significant, then the anti-inflammatory work of milk thistle extract and the ability to promote skin barrier repair are matched, the three can synergize, and black tea fermentation liquid, olive leaf extract and milk thistle extract are all natural plant extracts, and are safe and high, and are suitable for sensitive skin use. Therefore, after the three are compounded, not only safety is high, but also synergistic effect has significantly improved overall oil control and anti-acne efficacy, and acne problem can be solved more comprehensively.

[0009] Preferably, the oil-controlling and anti-acne composition comprises the following components in parts by weight: 5-10 parts of black tea fermentation liquid, 10.25-20 parts of olive leaf extract, and 0.45-0.8 parts of milk thistle extract.

[0010] When formulated within the above specific range, the oil-controlling and acne-removing composition has a more excellent synergistic effect.

[0011] More preferably, the oil-controlling and anti-acne composition comprises the following components in parts by weight: 5 parts of black tea fermentation liquid, 10.25 parts of olive leaf extract, and 0.45 parts of milk thistle extract.

[0012] In a second aspect, the present invention provides use of the above-mentioned oil-controlling and anti-acne composition in the preparation of oil-controlling and anti-acne cosmetics.

[0013] In a third aspect, the present invention provides an oil-controlling and anti-acne cosmetic, comprising the above-mentioned oil-controlling and anti-acne composition and auxiliary materials.

[0014] Preferably, the oil-controlling and anti-acne composition accounts for 1.6%-30.8% of the mass of the oil-controlling and anti-acne cosmetic.

[0015] Preferably, the auxiliary material includes at least one of a humectant, a thickener, a chelating agent, a preservative, and a solvent.

[0016] Preferably, the moisturizing agent includes at least one of glycerin, 1,2-pentanediol, and 1,2-hexanediol.

[0017] Preferably, the thickening agent comprises xanthan gum.

[0018] Preferably, the chelating agent comprises disodium EDTA.

[0019] Preferably, the preservative comprises p-hydroxyacetophenone.

[0020] Preferably, the solvent comprises water.

[0021] Preferably, the dosage form of the cosmetic includes essence, lotion, and cream.

[0022] The beneficial effects of the present invention are:

[0023] Black tea fermented liquor and olive leaf extract are susceptible to oxidation under light and high temperature conditions and cannot fully exert their efficacy; Milk thistle extract is widely used in health products and medicines, mainly for enhancing liver function and protecting the liver from damage, and has natural safety and multi-effect integration, but its oil control and anti-inflammatory aspects are relatively less studied. The present invention compounds black tea fermented liquor, olive leaf extract and milk thistle extract. After the three are combined, they synergize to form a stronger oil control network, and the oil control effect is greatly improved compared to when a single ingredient is used; the oil control and acne-removing composition obtained by compounding within the scope of the present invention has a significant effect of inhibiting Propionibacterium acnes, and the acne-removing effect is stronger; it can also further enhance the skin barrier function, reduce water loss, and make acne-damaged skin recover faster. Black tea fermented liquor, olive leaf extract and milk thistle extract are all natural plant extracts with high safety and suitable for use on sensitive skin. After the three are compounded, not only is the safety high, but the synergistic effect also significantly improves the oil control and acne-removing efficacy of the oil control and acne-removing composition, which can more comprehensively solve the acne problem. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 These are appearance diagrams of the oil-controlling and anti-acne compositions of Example 1, Example 3, and Example 6 (from left to right).

[0025] Figure 2 This is the 5α-reductase activity inhibition test result of the test sample.

[0026] Figure 3 These are the antibacterial test results of Propionibacterium acnes on the test samples.

[0027] Figure 4 These are the results of the hyaluronidase activity inhibition test in the positive control group.

[0028] Figure 5 It is the result of hyaluronidase activity inhibition test of the test sample. DETAILED DESCRIPTION

[0029] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0030] The black tea fermentation broth used in the present invention was purchased from Bozhou Mingqi Biotechnology Co., Ltd.; the olive leaf extract was purchased from Draco Natural Products; and the milk thistle extract was purchased from Givaudan.

[0031] The present invention has found in research that the extraction methods of olive leaf extract and milk thistle extract do not affect the efficacy of the present invention.

[0032] Examples 1-6:

[0033] Examples of the oil-control and anti-acne composition of the present invention; the components and their weight proportions of the oil-control and anti-acne composition of Examples 1-6 are shown in Table 1.

[0034] Table 1

[0035]

[0036] Comparative Examples 1-5:

[0037] Comparative Examples of the oil-controlling and anti-acne compositions of the present invention; the components and their weight proportions of the oil-controlling and anti-acne compositions of Comparative Examples 1-5 are shown in Table 2.

[0038] Table 2

[0039]

[0040] Comparative Examples 1-3 lack milk thistle extract, black tea fermentation broth, and olive leaf extract, respectively. Comparative Example 5 differs from Example 3 only in that an equal weight portion of milk thistle extract is replaced with white willow bark extract.

[0041] Application Examples 1-6:

[0042] Application Examples 1-6 provide an oil-controlling and anti-acne cosmetic, specifically an essence; the oil-controlling and anti-acne cosmetics of Application Examples 1-6 respectively include 5% of the oil-controlling and anti-acne composition of Examples 1-6; the formula of the oil-controlling and anti-acne cosmetic is shown in Table 3:

[0043] Table 3

[0044] Components content(%) glycerin 1.00 Xanthan gum 2.50 water To 100 1,2-Pentanediol 3.00 Disodium EDTA 0.07 1,2-Hexanediol 0.50 p-Hydroxyacetophenone 0.40 Oil-control and acne-removing composition 5

[0045] The preparation method of the oil-controlling and acne-removing cosmetic comprises the following steps:

[0046] (1) Preparation: Clean and disinfect the equipment and utensils used, and dry them for later use;

[0047] (2) Preparation of oil phase: Glycerin and xanthan gum were mixed uniformly to obtain mixture A;

[0048] (3) Preparation of aqueous phase: Add water, 1,2-pentanediol, disodium EDTA, 1,2-hexanediol, and p-hydroxyacetophenone into a stirring pot, heat to 75-80°C, and stir to disperse uniformly to obtain mixture B;

[0049] (4) Homogenization: Add the mixed mixture A to the mixture B, then homogenize until it is completely dispersed (1000-1500 rpm), keep warm for 15 minutes, and stir to cool to obtain the mixture C;

[0050] (5) After the mixture C is cooled to 40° C., the oil-control and acne-removing composition is added and stirred evenly (300-500 rpm); the oil-control and acne-removing cosmetic is obtained;

[0051] (6) Take samples for inspection. After the test is qualified, filter and discharge the material when the temperature drops to 38℃. After discharge, let it stand and inspect the semi-finished product. After passing the inspection, fill and package. After the finished product passes the inspection, put it into storage.

[0052] Application Comparative Examples 1-5:

[0053] Comparative Examples 1-5 are used to provide an oil-controlling and anti-acne cosmetic, specifically an essence; the oil-controlling and anti-acne cosmetics described in Comparative Examples 1-5 respectively include 5% of the oil-controlling and anti-acne composition described in Comparative Examples 1-5; the formula of the oil-controlling and anti-acne cosmetic is the same as that in Table 3.

[0054] The preparation method of the oil-controlling and anti-acne cosmetic is the same as that of Application Example 1.

[0055] Test Example 1: Stability Test

[0056] Test samples: the oil-controlling and anti-acne cosmetics (essences) prepared in Examples 1, 3, and 5 and Comparative Examples 1-3.

[0057] After the samples have passed the inspection of appearance and physical and chemical indicators, they will be divided into the following two test groups for testing:

[0058] Normal temperature test group: stored at 24-25°C for 7 days, 1 month, 2 months, and 3 months;

[0059] Heat resistance test group: stored at 45-48℃ for 7 days, 1 month, 2 months and 3 months respectively.

[0060] The test samples in each test group were observed on the 7th day, 1 month, 2 months, and 3 months after the corresponding treatment. The observation items were texture, color, aroma, skin feel, and viscosity.

[0061] The stability test observation cycle is usually one month. The sample status is observed regularly every month. The heat resistance test group samples are compared with the room temperature test group samples. The material status is comprehensively considered and the results are recorded in the "Formula Stability Test Report". The test results are shown in Table 4.

[0062] Table 4 Stability observation results

[0063]

[0064] Test Example 2: Oil Control Efficacy Test

[0065] This test is based on the 5α-reductase inhibition test to evaluate the oil-control efficacy of the sample. The test is carried out according to the in vitro test method for the oil-control efficacy of cosmetics (5α-reductase inhibition method).

[0066] Test sample: oil-control and acne-removing composition prepared in Examples 1, 3, and 6 (its appearance is as follows Figure 1 shown).

[0067] Test method:

[0068] 1. Main reagents: 5α-reductase, testosterone, tetrasodium salt of reduced coenzyme II (NADPH), finasteride; Main equipment: multifunctional microplate reader (Bio Tek, model Cytation 1), electronic balance (Sartiorius, model BSA-224S).

[0069] 2. Carry out group testing according to Table 5.

[0070] Table 5 Test groups

[0071]

[0072]

[0073] 3. Solution preparation:

[0074] (3.1) Testosterone solution: Weigh 5.768 mg of testosterone and dissolve it in 100 mL of PBS buffer solution to prepare a 200 μmol / L testosterone solution. The final test concentration is 20 μmol / L.

[0075] (3.2) NADPH solution: Weigh 1.640 mg of NADPH and dissolve it in 100 mL of PBS buffer to prepare a 22 μmol / L NADPH solution.

[0076] (3.3) Positive control (finasteride solution): Weigh 2.500 mg of finasteride and dissolve it in 100 mL of deionized water to prepare a finasteride solution with a concentration of 25 μg / mL.

[0077] 4. Add samples in sequence according to Table 6, mix, incubate at 37°C, and use a microplate reader to detect the absorbance change of each test group at 340 nm within 10 minutes. Record and save the data.

[0078] Table 6 Sample addition list

[0079]

[0080] 5. Calculate the 5α-reductase inhibition rate according to the following formula:

[0081]

[0082] In the above formula:

[0083] ΔA—absorbance change value of the normal enzyme reaction group (containing PBS solution, testosterone solution, enzyme extract, and NADPH solution);

[0084] ΔB—absorbance change value of the experimental group (containing PBS solution, testosterone solution, sample solution (sample group) / finasteride solution (positive control group) / water (negative control group), enzyme extract, and NADPH solution);

[0085] ΔC—absorbance change value of the blank group (containing PBS solution, testosterone solution, and NADPH solution).

[0086] The test results are shown in Table 7 and Figure 2 The oil-control and acne-removing compositions prepared in Examples 1, 3, and 6 all have a significant effect of inhibiting the activity of 5α-reductase, that is, the oil-control and acne-removing compositions prepared within the ratio range of the present invention all have a significant oil-control effect.

[0087] Table 7 5α-reductase activity inhibition rate test results

[0088] Test samples 5α-reductase activity inhibition rate (%) Positive control group 52.41±2.07 Negative control group 3.52±1.12 Example 1 57.41±2.15 Example 3 75.00±2.46 Example 6 78.67±3.12

[0089] Test Example 3: Acne Treatment Efficacy Test

[0090] This test example tests the acne-removing efficacy of cosmetics using the in vitro anti-acne efficacy test method (bacterial inhibition rate method). Because acne is primarily caused by the accumulation of Propionibacterium acnes, the antibacterial effect of the sample on P. acnes can be used to evaluate the sample's acne-removing efficacy.

[0091] Test samples: oil-controlling and anti-acne cosmetics (essences) prepared in Application Examples 1, 3, and 6 and Comparative Examples 1-5; negative control group: sterile standard hard water.

[0092] Test method:

[0093] 1. Main reagents: Clostridium enrichment medium, phosphate buffered saline (PBS), standard strain: Propionibacterium acnes ATCC6919.

[0094] 2. Main equipment: biological safety cabinet (brand Lubo, model LB-9100), electronic balance (brand Sartiorius, model BSA-224S), biochemical incubator (brand Thermo, model RI-150).

[0095] 3. Experimental steps

[0096] (3.1) Dilute the test bacterial solution appropriately with PBS solution to the following concentration: take 0.1 mL and drop it into 5.0 mL of control solution (PBS). The number of recovered bacteria is 1×10 4 ~9×10 4 CFU / mL; dilute the test sample with sterile standard hard water to the specified concentration;

[0097] (3.2) Pipette 5 mL of the original sample solution or its dilution into a sterile test tube. Pipette 0.1 mL of the above test bacterial solution into the test tube containing 5 mL of the sample, mix quickly, and start the time immediately.

[0098] (3.3) After 20 minutes, add 0.5 mL of the above mixture into a test tube containing 4.5 mL of sterilized PBS and mix thoroughly.

[0099] (3.4) After 10 minutes, pipette 1 mL of the sample solution into a sterile plate. Inoculate two sterile plates for each sample solution or dilution.

[0100] (3.5) Pour 15 mL of Clostridium enrichment medium cooled to 40°C–45°C into the plate and rotate the plate to mix thoroughly. After the medium solidifies, flip the plate over and incubate anaerobically at 37°C in an incubator for 72 h. Count the viable colonies.

[0101] (3.6) Substitute PBS for the test sample and follow the above steps as a blank control group. Repeat the experiment three times and calculate the average value.

[0102] (3.7) A negative control group (bacterial suspension, culture medium, sterile standard hard water) must be prepared separately for the test.

[0103] 4. Calculate the antibacterial rate according to the following formula:

[0104]

[0105] In the above formula:

[0106] A—average colony count of blank control samples;

[0107] B—average colony count of experimental group;

[0108] The test results are shown in Table 8 and Figure 3 shown.

[0109] Table 8 Sample test results

[0110] Test samples Antibacterial rate (%) Negative control group 8.58±0.35 Application Example 1 80.36±0.31 Application Example 3 86.95±0.44 Application Example 6 93.88±0.28 Comparative Application Example 1 60.12±0.36 Application Comparative Example 2 57.98±0.32 Application Comparative Example 3 50.41±0.37 Comparative Application Example 4 53.39±0.24 Application Comparative Example 5 70.15±0.41

[0111] Table 8 and Figure 3 The results showed that compared with the negative control, the essence containing the oil-control and acne-removing composition can effectively inhibit Propionibacterium acnes and achieve excellent acne-removing effects.

[0112] Compared with Application Examples 1, 3, and 6, the essences of Application Examples 3 and 6 have better acne-removing effects, and Application Example 3 has a relatively higher cost-effectiveness.

[0113] Compared with the application examples, the essences of Comparative Examples 1-3 lack any of the active ingredients in the oil-control and acne-removing composition. Even if the dosage of the other two active ingredients is increased, the effect is not as good as the combination of the three active ingredients, indicating that the three have a synergistic effect. In Comparative Examples 4 and 5, the composition ratio of the oil-control and acne-removing composition exceeds the scope of the present invention, or the milk thistle extract is replaced with the white willow bark extract with the same efficacy. The antibacterial rate is significantly reduced, indicating that the black tea fermented liquid, olive leaf extract, and milk thistle extract of the present invention are a specific combination, and only when compounded at a specific weight ratio can they have excellent acne-removing efficacy.

[0114] Test Example 4: Anti-inflammatory and repair efficacy test

[0115] The samples' inhibitory effects on hyaluronidase activity were tested to evaluate their anti-inflammatory and repair abilities.

[0116] Test principle:

[0117] Hyaluronidase is an endogenous hexosaminidase that degrades macromolecular hyaluronic acid, producing N-acetylglucosamine. Hyaluronidase hydrolyzes the 1,4-glycosidic bond between β-N-acetylglucosamine and D-glucuronic acid in hyaluronic acid, yielding β-N-acetylglucosamine. Under alkaline conditions, β-N-acetylglucosamine condenses with acetylacetone to form the chromogenic 2-methyl-3-acetylpyrrole derivative. The chromogenic chromogen reacts with dimethylaminobenzaldehyde in concentrated hydrochloric acid-ethanol to produce color. Therefore, measuring the N-acetylglucosamine content in the reaction system can indirectly reflect hyaluronidase activity. Hyaluronidase is strongly associated with inflammation, so inhibition of hyaluronidase activity is used as an indicator for studying anti-inflammatory and repairing effects.

[0118] Test method:

[0119] 1. Instruments: UV spectrophotometer U2910 (HITACHI), electronic balance BSA-224S (Sartiorius).

[0120] 2. Test materials

[0121] (2.1) Main reagents:

[0122] Sodium acetate, glacial acetic acid, anhydrous calcium chloride, sodium hyaluronate, hyaluronidase, p-dimethylaminobenzaldehyde, concentrated hydrochloric acid, anhydrous ethanol, sodium carbonate, sodium hydroxide, acetylacetone, dipotassium glycyrrhizate (≥98%).

[0123] (2.2) Sample grouping

[0124] Test sample: the oil-controlling and anti-acne cosmetics (essence) prepared in Examples 1, 3, and 6.

[0125] Control group / positive group: dipotassium glycyrrhizate solution (prepared with deionized water to prepare dipotassium glycyrrhizate solutions with mass concentrations of 5%, 0.3%, and 0.1%, respectively).

[0126] 3. Experimental steps

[0127] (3.1) Solution preparation:

[0128] Acetic acid-sodium acetate buffer (pH = 5.6): Measure 1.155 mL of glacial acetic acid, dilute to 100 mL, and mix thoroughly to prepare acetic acid solution; weigh 2.72 g of sodium acetate, dissolve in water, and dilute to 100 mL to prepare sodium acetate solution; add 4.8 mL of acetic acid solution to 45.2 mL of sodium acetate solution, mix thoroughly, and dilute to 100 mL to prepare acetic acid-sodium acetate buffer (pH = 5.6).

[0129] Sodium hyaluronate solution: Weigh 0.0050 g of sodium hyaluronate and dissolve it in 10 mL of acetic acid-sodium acetate buffer (pH = 5.6) to prepare a sodium hyaluronate solution with a mass concentration of 0.5 mg / mL.

[0130] Ehrlich reagent: Weigh 0.8 g of p-dimethylaminobenzaldehyde and dissolve it in 15 mL of concentrated hydrochloric acid and 15 mL of anhydrous ethanol. Protect from light.

[0131] CaCl2 solution: Weigh 2.8 g of anhydrous calcium chloride and dissolve it in 10 mL of deionized water. Ultrasonicate until completely dissolved.

[0132] NaOH solution: Weigh 0.16 g of NaOH and dissolve it in 10 mL of deionized water. Ultrasonicate until completely dissolved.

[0133] Hyaluronidase solution: Weigh 0.0100 g of hyaluronidase and dissolve it in 4 mL of acetic acid-sodium acetate buffer to prepare a hyaluronidase solution with a working concentration of 1250 U / mL. Prepare it before use.

[0134] Acetylacetone solution: Measure 3.5 mL of acetylacetone and dissolve it in 50 mL of sodium carbonate solution (1.0 mol / L) to prepare acetylacetone solution. Prepare it before use.

[0135] (3.2) Add samples in sequence according to Table 9.

[0136] Table 9 Sample addition list

[0137]

[0138]

[0139] The solution to be tested was transferred into a 3 cm cuvette, the absorbance of each group was detected at 547 nm, and the data were recorded.

[0140] 4. Calculate the inhibition rate according to the following formula:

[0141]

[0142] In the above formula:

[0143] A-absorbance value of control solution (acetic acid-sodium acetate buffer instead of sample solution);

[0144] B-absorbance value of blank control solution (acetic acid-sodium acetate buffer instead of sample solution and enzyme solution);

[0145] C-absorbance value of sample solution;

[0146] D-absorbance value of the blank solution of the sample (acetic acid-sodium acetate buffer instead of enzyme solution).

[0147] 5. Test results:

[0148] Table 10 Dipotassium glycyrrhizinate solution (positive control group) test results

[0149] Test samples Inhibition rate (%) 0.1% dipotassium glycyrrhizate 51.87±1.77 0.3% dipotassium glycyrrhizate 66.54±1.32 5.0% dipotassium glycyrrhizate 86.57±1.86

[0150] Table 11 Sample test results

[0151] Test samples Inhibition rate (%) Application Example 1 30.03±1.71 Application Example 3 59.75±0.86 Application Example 6 75.75±0.47

[0152] The results are shown in Tables 10-11. Figure 4-5 As shown, the oil-control and acne-removing cosmetics prepared using Examples 1, 3, and 6 all have the effect of inhibiting the activity of hyaluronidase (i.e., anti-inflammatory and repairing effects); the effects of Examples 3 and 6 are particularly significant.

[0153] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. An oil-controlling and acne-removing composition, characterized in that: The invention comprises the following components in parts by weight: 1-10 parts of black tea fermentation liquid, 0.5-20 parts of olive leaf extract and 0.1-0.8 parts of milk thistle extract.

2. The oil-control and acne-removing composition according to claim 1, wherein: The invention comprises the following components in parts by weight: 5-10 parts of black tea fermentation liquid, 10.25-20 parts of olive leaf extract and 0.45-0.8 parts of milk thistle extract.

3. The oil-control and acne-removing composition according to claim 2, wherein: The invention comprises the following components in parts by weight: 5 parts of black tea fermentation liquid, 10.25 parts of olive leaf extract and 0.45 parts of milk thistle extract.

4. Use of the oil-controlling and anti-acne composition according to any one of claims 1 to 3 in the preparation of oil-controlling and anti-acne cosmetics.

5. An oil-controlling and acne-removing cosmetic, characterized in that: The invention comprises the oil-controlling and anti-acne composition according to any one of claims 1 to 3 and auxiliary materials.

6. The oil-controlling and anti-acne cosmetic according to claim 5, characterized in that: The oil-controlling and anti-acne composition accounts for 1.6% to 30.8% of the mass of the oil-controlling and anti-acne cosmetic.

7. The oil-controlling and anti-acne cosmetic according to claim 5, characterized in that: The auxiliary materials include at least one of a moisturizer, a thickener, a chelating agent, a preservative, and a solvent.

8. The oil-controlling and anti-acne cosmetic according to claim 7, characterized in that: The moisturizing agent includes at least one of glycerin, 1,2-pentanediol, and 1,2-hexanediol; and / or, the thickener comprises xanthan gum; and / or, the chelating agent comprises disodium EDTA; and / or, the preservative comprises p-hydroxyacetophenone; And / or, the solvent comprises water.

9. The oil-controlling and anti-acne cosmetic according to claim 5, characterized in that: The dosage forms of the cosmetics include essence, lotion and cream.

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