Efficient moisturizing and repairing composition and preparation method thereof

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to an efficient moisturizing and repairing composition and a preparation method thereof. The efficient moisturizing and repairing composition provided by the invention is formed by compounding the artemisia capillaris extract, the L-rhamnose, the soluble proteoglycan and the succinyl glycan, has better moisturizing and repairing effects, is mild and non-irritant, and can be used for preparing efficient moisturizing and repairing products; the preparation method of the artemisia capillaris extract adopts the modes of freezing, ultrahigh pressure treatment and flash extraction, has the advantages of improving the extraction efficiency and avoiding degradation of active ingredients, is easy to operate and relatively low in cost, and can also retain the active ingredients to the greatest extent. The preparation method of the efficient moisturizing and repairing composition provided by the invention is simple and easy to operate, is beneficial to industrial production and application, and has a wide application prospect.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to a high-efficiency moisturizing and repairing composition and a preparation method thereof. Background Art

[0002] In modern society, people generally face high work or study pressure. When people are under high pressure for a long time, as well as changes and stimulation from the external climate environment, the secretion of sebaceous glands and sweat glands in the skin will become abnormal. As the skin ages, sebum secretion decreases, and the skin's ability to retain moisture decreases, resulting in dry skin and thickening of the stratum corneum. This can lead to problems such as keratinization, dryness, cracking, and roughness of the skin surface. In recent years, people's awareness of beauty has gradually increased. Therefore, how to moisturize and repair the skin has become a research hotspot in the field of current skin care.

[0003] Currently, existing cosmetic compositions mainly achieve the effect of soothing and repairing the skin by adding moisturizers, such as hyaluronic acid, small molecule polyols, polysaccharides and other substances, by increasing the moisture in the skin; or by adding ceramides to replenish skin ceramides to achieve the effect of repairing the skin barrier. Although the above-mentioned existing methods have a certain soothing and repairing effect on the skin, there are also some problems. For example, moisturizers are often only short-term hydration, and the skin repair effect is very limited; although ceramides can repair the skin barrier, they cannot effectively and timely relieve allergic reactions of the skin; some skin care products contain more ethanol and other substances that are irritating to the skin, which can easily cause skin allergies during actual use; some compositions have poor stability and can only achieve epidermal repair.

[0004] In summary, in order to better achieve moisturizing and repairing effects, it is necessary to develop a composition that has good moisturizing and repairing effects and is mild and non-irritating. Summary of the Invention

[0005] In response to the above defects or improvement needs of the prior art, the present invention provides a high-efficiency moisturizing and repairing composition and a preparation method thereof, which solve the problems that the existing moisturizing and repairing compositions have limited effects and strong irritation.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions: In a first aspect, the present invention provides a high-efficiency moisturizing and repairing composition, comprising the following components by weight: 14-18 parts of Artemisia capillaris extract, 7-11 parts of L-rhamnose, 9-13 parts of soluble proteoglycan, and 5-9 parts of succinoglycan; The preparation method of the Artemisia capillaris extract comprises the following steps: A. Take dried Artemisia capillaris, crush it, and pass it through a 30-mesh sieve to obtain Artemisia capillaris powder; B. adding ethanol solution to the Artemisia capillaris powder, mixing uniformly, freezing, and then performing ultrahigh pressure treatment to obtain a mixture; C. placing the mixture in a flash extractor for flash extraction to obtain an extract; D. Centrifuge the extract, take the supernatant and rotary evaporate until it is free of alcohol to obtain the Artemisia capillaris extract.

[0007] Artemisia capillaris extract is rich in various active ingredients such as flavonoids, phenols, organic acids, volatile oils, terpenes, etc., and has multiple physiological activities such as liver protection and choleretic, anti-inflammatory, antioxidant, antiviral, anti-tumor, and lipid metabolism regulation.

[0008] L-rhamnose is a naturally occurring deoxysugar that has the properties of strengthening the skin barrier, anti-inflammatory and soothing, and antioxidant. It can also improve the permeability of other active ingredients.

[0009] Soluble proteoglycan, a proteoglycan solution extracted from salmon nasal cartilage tissue, has one or more covalent bonds that can connect to mucopolysaccharides and has a long-lasting moisturizing effect.

[0010] Succinoglycan is a type of extracellular polysaccharide secreted by nitrogen-fixing microorganisms such as rhizobia. It has a moisturizing effect and can reduce the response of inflammatory factors, enhance the immune activity of epidermal cells, and thus enhance the resistance of the skin barrier.

[0011] Ultra-high pressure treatment after freezing treatment helps to break the cell wall efficiently, promote the release of active ingredients, and significantly improve the extraction efficiency of active ingredients. At the same time, low temperature can inhibit enzyme activity and oxidation reactions to a certain extent, thereby avoiding the degradation of extracted active ingredients. The operation is simple and suitable for industrial production.

[0012] Flash extraction is a technology for extracting active ingredients from plants. It will not damage the active ingredients due to excessively high temperature or long extraction time. It can retain the active ingredients to the greatest extent possible and has the advantages of easy operation, low cost and high extraction efficiency.

[0013] Preferably, the composition comprises the following components by weight: 16 parts of Artemisia capillaris extract, 9 parts of L-rhamnose, 11 parts of soluble proteoglycan, and 7 parts of succinoglycan.

[0014] Preferably, the volume fraction of the ethanol solution in step B is 55-65%, and the solid-liquid ratio of Artemisia capillaris powder to ethanol solution is 1 g:12-17 mL.

[0015] Preferably, the freezing treatment in step B is performed at a temperature of -20 to -15°C and for a time of 22 to 32 minutes.

[0016] Preferably, the pressure of the ultrahigh pressure treatment in step B is 250-350 MPa, the number of ultrahigh pressure treatments is 2-4 times, and the time of each ultrahigh pressure treatment is 40-60 seconds.

[0017] Preferably, the voltage of the flash extraction in step C is 90-110 V, the number of flash extractions is 2-4 times, the time of each flash extraction is 30-45 seconds, and the speed of the flash extraction is 4000-5000 rpm.

[0018] In a second aspect, the present invention provides a method for preparing the composition of the first aspect, the method comprising the following steps: weighing each component according to the formula amount, mixing and homogenizing to obtain the composition.

[0019] In a third aspect, the present invention provides a use of the composition described in the first aspect or the composition prepared by the preparation method described in the second aspect in the preparation of a high-efficiency moisturizing and repairing product.

[0020] Preferably, the highly effective moisturizing and repairing product is an essence, gel, lotion, facial mask or facial cream.

[0021] Beneficial effects of the present invention: 1. The present invention provides a high-efficiency moisturizing and repairing composition, which is composed of a compound of Artemisia capillaris extract, L-rhamnose, soluble proteoglycan and succinoglycan; Artemisia capillaris extract has anti-inflammatory and antioxidant effects, L-rhamnose has the properties of enhancing skin barrier, anti-inflammatory and soothing, and antioxidant, soluble proteoglycan has a long-lasting moisturizing effect, and succinoglycan has the effects of moisturizing, anti-inflammatory, and enhancing the resistance of the skin barrier. The four ingredients synergistically improve the moisturizing, anti-inflammatory, antioxidant and repair effects.

[0022] 2. The present invention provides a method for preparing an Artemisia capillaris extract, wherein freezing and ultra-high pressure treatment are both helpful to rupture the cell walls, thereby improving the extraction efficiency and avoiding degradation of active ingredients; combined with flash extraction, it has the advantages of easy operation, low cost and high extraction efficiency, and can also retain the active ingredients to the maximum extent.

[0023] 3. The present invention provides a preparation method of the high-efficiency moisturizing and repairing composition, which is simple and easy to operate and is conducive to industrial production and application.

[0024] 4. The present invention provides the use of the high-efficiency moisturizing and repairing composition in the preparation of high-efficiency moisturizing and repairing products, which has broad application prospects. DETAILED DESCRIPTION

[0025] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined purpose of the invention, the following is a detailed description of the specific implementation methods, structures, features and effects of the present invention in combination with the composition.

[0026] For the following compositions, experimental methods where specific conditions are not specified were generally performed under conventional conditions or those recommended by the manufacturer. Materials and reagents used were commercially available unless otherwise specified.

[0027] Some of the raw materials and their sources are as follows: Artemisia capillaris was purchased from Shanghai Xingye Biotechnology Co., Ltd. L-rhamnose was purchased from Shanghai Xingye Biotechnology Co., Ltd.; Soluble proteoglycan was purchased from Shanghai Xingye Biotechnology Co., Ltd.; Succinoglycan was purchased from Shanghai Xingye Biotechnology Co., Ltd.

[0028] The preparation method of Artemisia capillaris extract ① comprises the following steps: A. Take dried Artemisia capillaris, crush it, and pass it through a 30-mesh sieve to obtain Artemisia capillaris powder; B. adding ethanol solution to the Artemisia capillaris powder, mixing uniformly, freezing, and then performing ultrahigh pressure treatment to obtain a mixture; C. placing the mixture in a flash extractor for flash extraction to obtain an extract; D. centrifuging the extract, taking the supernatant and rotary evaporating it until it is free of alcohol, thereby obtaining the Artemisia capillaris extract; The volume fraction of the ethanol solution in step B is 65%, the solid-liquid ratio of Artemisia capillaris powder to the ethanol solution is 1 g:12 mL; the freezing treatment temperature is -20°C and the time is 22 min; the pressure of the ultrahigh pressure treatment is 250 MPa, the number of ultrahigh pressure treatments is 4, and the time of each ultrahigh pressure treatment is 60 s; In step C, the voltage of the flash extraction is 110 V, the number of flash extractions is 2, the time of each flash extraction is 30 s, and the speed of the flash extraction is 5000 rpm.

[0029] The preparation method of Artemisia capillaris extract ② comprises the following steps: A. Take dried Artemisia capillaris, crush it, and pass it through a 30-mesh sieve to obtain Artemisia capillaris powder; B. adding ethanol solution to the Artemisia capillaris powder, mixing uniformly, freezing, and then performing ultrahigh pressure treatment to obtain a mixture; C. placing the mixture in a flash extractor for flash extraction to obtain an extract; D. centrifuging the extract, taking the supernatant and rotary evaporating it until it is free of alcohol, thereby obtaining the Artemisia capillaris extract; The volume fraction of the ethanol solution in step B is 55%, the solid-liquid ratio of Artemisia capillaris powder to the ethanol solution is 1 g:17 mL; the freezing treatment temperature is -15 ° C and the time is 32 min; the pressure of the ultrahigh pressure treatment is 350 MPa, the number of ultrahigh pressure treatments is 2, and the time of each ultrahigh pressure treatment is 40 s; In step C, the voltage of the flash extraction is 90 V, the number of flash extractions is 4 times, the time of each flash extraction is 45 seconds, and the speed of the flash extraction is 4000 rpm.

[0030] The preparation method of Artemisia capillaris extract ③ comprises the following steps: A. Take dried Artemisia capillaris, crush it, and pass it through a 30-mesh sieve to obtain Artemisia capillaris powder; B. adding ethanol solution to the Artemisia capillaris powder, mixing uniformly, freezing, and then performing ultrahigh pressure treatment to obtain a mixture; C. placing the mixture in a flash extractor for flash extraction to obtain an extract; D. centrifuging the extract, taking the supernatant and rotary evaporating it until it is free of alcohol, thereby obtaining the Artemisia capillaris extract; The volume fraction of the ethanol solution in step B is 60%, and the solid-liquid ratio of Artemisia capillaris powder to the ethanol solution is 1 g:14 mL; the freezing treatment temperature is -18 ° C and the time is 27 min; the pressure of the ultrahigh pressure treatment is 300 MPa, the number of ultrahigh pressure treatments is 3 times, and the time of each ultrahigh pressure treatment is 50 s; In step C, the voltage of the flash extraction is 100 V, the number of flash extractions is 3 times, the time of each flash extraction is 40 seconds, and the speed of the flash extraction is 4500 rpm.

[0031] The only difference between Artemisia capillaris extract ④ and Artemisia capillaris extract ③ is that the volume fraction of the ethanol solution in the preparation step B of Artemisia capillaris extract ④ is 50%, and other conditions are the same as those of Artemisia capillaris extract ③.

[0032] The difference between Artemisia capillaris extract ⑤ and Artemisia capillaris extract ③ is that the volume fraction of the ethanol solution in the preparation step B of Artemisia capillaris extract ⑤ is 70%, and other conditions are the same as those of Artemisia capillaris extract ③.

[0033] The difference between Artemisia capillaris extract ⑥ and Artemisia capillaris extract ③ is that the pressure of ultrahigh pressure treatment in the preparation step B of Artemisia capillaris extract ⑥ is 200 MPa, and other conditions are the same as those of Artemisia capillaris extract ③.

[0034] The difference between Artemisia capillaris extract ⑦ and Artemisia capillaris extract ③ is that the pressure of ultrahigh pressure treatment in the preparation step B of Artemisia capillaris extract ⑦ is 400 MPa, and other conditions are the same as those of Artemisia capillaris extract ③.

[0035] The difference between Artemisia capillaris extract ⑧ and Artemisia capillaris extract ③ is only that the preparation step B of Artemisia capillaris extract ⑧ is different, and the other conditions are the same as those of Artemisia capillaris extract ③; Step B is specifically as follows: adding ethanol solution to Artemisia capillaris powder, mixing evenly, and soaking at room temperature for 6 hours to obtain a mixture; wherein the volume fraction of the ethanol solution in step B is 60%, and the solid-liquid ratio of Artemisia capillaris powder and ethanol solution is 1g:14mL.

[0036] The components (parts by weight) of the high-efficiency moisturizing and repairing compositions of Examples 1-3 of the present invention are shown in Table 1 below. Each component is weighed according to the formula amount, mixed and homogenized to obtain each composition.

[0037] Table 1 Components and weight percentages of the high-efficiency moisturizing and repairing compositions of Examples 1-3 Example Artemisia capillaris extract Artemisia capillaris extract L-Rhamnose Soluble proteoglycans Succinoglycan 1 ① 14 11 9 9 2 ② 18 7 13 5 3 ③ 16 9 11 7 Comparative Example 1: Compared with Example 3, the difference is that in Comparative Example 1, no Artemisia capillaris extract is added, and the missing amount is supplemented with L-rhamnose, soluble proteoglycan and succinoglycan in a weight ratio of 9:11:7. Other conditions are the same as in Example 3.

[0038] Comparative Example 2: Compared with Example 3, the difference is that L-rhamnose is not added in Comparative Example 2, and the missing amount is made up with Artemisia capillaris extract, soluble proteoglycan and succinoglycan in a weight ratio of 16:11:7. Other conditions are consistent with Example 3.

[0039] Comparative Example 3: Compared with Example 3, the difference is that no soluble proteoglycan is added in Comparative Example 3, and the missing amount is supplemented by Artemisia capillaris extract, L-rhamnose and succinoglycan in a weight ratio of 16:9:7. Other conditions are consistent with Example 3.

[0040] Comparative Example 4: Compared with Example 3, the difference is that no succinoglycan is added in Comparative Example 4, and the missing amount is supplemented by Artemisia capillaris extract, L-rhamnose and soluble proteoglycan in a weight ratio of 16:9:11. Other conditions are consistent with Example 3.

[0041] Comparative Example 5: Compared with Example 3, the difference is that in Comparative Example 5, Artemisia capillaris extract ④ is used instead of Artemisia capillaris extract ③, and other conditions are the same as those in Example 3.

[0042] Comparative Example 6: Compared with Example 3, the difference is that in Comparative Example 6, Artemisia capillaris extract ⑤ is used instead of Artemisia capillaris extract ③, and other conditions are the same as those in Example 3.

[0043] Comparative Example 7: Compared with Example 3, the difference is that in Comparative Example 7, Artemisia capillaris extract ⑥ is used instead of Artemisia capillaris extract ③, and other conditions are the same as those in Example 3.

[0044] Comparative Example 8: Compared with Example 3, the difference is that in Comparative Example 8, Artemisia capillaris extract ⑦ is used instead of Artemisia capillaris extract ③, and other conditions are the same as those in Example 3.

[0045] Comparative Example 9: Compared with Example 3, the difference is that in Comparative Example 9, Artemisia capillaris extract ⑧ is used instead of Artemisia capillaris extract ③, and other conditions are the same as those in Example 3.

[0046] Test Example 1 Hyaluronidase Inhibition Experiment Hyaluronic acid is a powerful moisturizing agent that can attract and lock moisture, maintain skin moisture, and improve dry, rough and dehydrated skin conditions;And hyaluronic acid can also enhance the biological activity of cells, for repairing and protecting skin with a strong synergistic effect. Hyaluronidase is a class of enzymes that can hydrolyze hyaluronic acid, widely present in human tissue, and the increase of its activity can cause the decomposition of hyaluronic acid, so as to reduce the moisturizing ability to skin, and the activity of hyaluronidase can slow down the decomposition of hyaluronic acid, so as to maintain the content of hyaluronic acid in skin, enhance the moisturizing and repair ability of skin. Therefore, this test example is to the inhibition rate of hyaluronidase by test composition, and then evaluates the moisturizing effect of the high-efficiency moisturizing repair composition prepared by Example 1-3 and Comparative Example 1-9.

[0047] Test samples: the high-efficiency moisturizing and repairing compositions prepared in Examples 1-3 and Comparative Examples 1-9 of the present invention, diluted with DMSO to a concentration of 4.5% for use; The test specifically includes the following steps: 1.1 Take four sets of clean test tubes, labeled A, B, C, and D. Add 50 μL of the sample to be tested to test tubes A and B, 50 μL of distilled water to test tubes C and D, 50 μL of 500 U / mL hyaluronidase solution to test tubes A and C, and 50 μL of pH 5.6 acetate buffer to test tubes B and D. Place the test tubes in a 37°C incubator for 20 min. 1.2 Add 10 μL of 2.5 mol / L CaCl2 solution to each of the four test tubes and then place in a 37°C incubator for 20 min. 1.3 Add 50 μL of 0.5 mg / mL sodium hyaluronate solution to test tubes A and C, and add 50 μL of pH 5.6 acetate buffer to test tubes B and D. Place the test tubes in a 37°C incubator for 40 minutes, then remove them and allow them to stand at room temperature for 10 minutes. 1.4 Add 50 μL of distilled water, 10 μL of 5 mol / L NaOH solution, and 50 μL of acetylacetone solution to four test tubes respectively. Place the test tubes in a boiling water bath for 30 min, then in an ice bath for 10 min, and finally at room temperature for 10 min. 1.5 Add 100 μL of P-DAB reagent to each test tube and measure the absorbance at 530 nm using a microplate reader; The hyaluronidase inhibition rate was calculated as follows: ; Where: A is the OD value of the sample solution; B is the OD value of the sample blank; C is the OD value of the control sample solution; D is the OD value of the control sample blank; each group was measured in parallel 3 times, and the data are presented as the average value; The hyaluronidase inhibition rate data are shown in Table 2 below.

[0048] Table 2 Hyaluronidase inhibition rate data Group Hyaluronidase inhibition rate (%) Example 1 93.36 Example 2 90.78 Example 3 94.02 Comparative Example 1 68.17 Comparative Example 2 76.42 Comparative Example 3 72.37 Comparative Example 4 74.19 Comparative Example 5 86.24 Comparative Example 6 87.57 Comparative Example 7 85.46 Comparative Example 8 88.29 Comparative Example 9 80.35 Test Example 2 Cell scratch test The human immortalized epidermal cell line (HaCaT), derived from keratinocytes from adult human skin, exhibits characteristics such as adherent growth, stable morphology, and excellent differentiation capacity. Because it can acquire unlimited proliferation through spontaneous mutation, it serves as an important in vitro model for studying human epidermal function, skin disease mechanisms, inflammatory responses, regeneration and repair, and for evaluating cosmetics and pharmaceuticals.

[0049] Cell migration refers to the movement of cells after receiving migration signals or sensing certain substances. It is involved in many physiological or pathological activities such as immunity, tumor metastasis, inflammation, and damage repair.

[0050] The cell scratch test artificially creates a blank area on a confluent monolayer of cells, called a "scratch / wound". Cells at the edge of the scratch gradually move into the blank area to heal the "scratch / wound". Images are captured regularly during the cell migration process. By measuring the scratch spacing at different time points and calculating the difference, the cell migration ability can be judged. This can be used to evaluate the effects of exogenous factors such as drugs and genes on cell migration, repair, and interactions.

[0051] Tested substances: the high-efficiency moisturizing and repairing compositions prepared in Examples 1-3 and Comparative Examples 1-9; Test substance treatment: dilute the test substance to a concentration of 0.005 mg / mL with DMEM culture medium for use; The test specifically includes the following steps: 2.1 Use a marker to evenly draw horizontal lines on the back of the 6-well plate, with a line every 0.5 cm across the wells, with 5 lines across each well. 2.2 Take HaCaT cells in the logarithmic growth phase and digest them with 0.25% trypsin to form a single cell suspension. The cell concentration is 6×10 5HaCaT cells at a concentration of 10 cells / mL were seeded into 6-well plates and DMEM medium containing 10% FBS was added. The plates were cultured in a cell culture incubator at 37°C and 5% CO2 for 24 h until a confluent cell monolayer was formed. At this point, two scratches were made in each well using a 200 μL pipette tip perpendicular to the horizontal line on the back of the 6-well plate. The plates were rinsed three times with PBS, the scratched cells were removed, and 1.8 mL of DMEM medium without FBS was added. The plates were photographed as the initial image of the cell scratch experiment. 2.3 Add 200 μL of the test substance to each well and continue to culture in a cell culture incubator at 37°C and 5% CO2 for 24 hours. Remove the cells and take pictures as the 24-hour chart of the cell scratch test. In the blank control group, 200 μL PBS was used instead of the test substance. Five replicate wells were set for each test substance group and blank control group. 2.4 Use Image J software to perform image statistical area analysis on the initial image and 24h image of the cell scratch experiment to obtain the initial scratch area and 24h scratch area respectively; 2.5 Calculate the cell migration rate according to the following formula: ; 2.6 Data Processing: SPSS statistical software was used for data analysis. All measured data are expressed as mean ± standard deviation. Statistical analysis was performed using the t-test, with P < 0.05 considered statistically significant.

[0052] The results of the cell scratch test are shown in Table 3 below. The higher the cell migration rate, the better the repair effect of the high-efficiency moisturizing and repairing composition prepared by the present invention on skin barrier damage; Table 3 Cell scratch test results Group Cell migration rate (%) Blank control 15.33±1.72 Example 1 <![CDATA[65.91±5.24 * ]]> Example 2 <![CDATA[63.32±6.01 * ]]> Example 3 <![CDATA[68.18±6.29 * ]]> Comparative Example 1 <![CDATA[40.13±3.87 *△ ]]> Comparative Example 2 <![CDATA[50.86±5.12 *△ ]]> Comparative Example 3 <![CDATA[46.25±4.53 *△ ]]> Comparative Example 4 <![CDATA[48.09±4.61 *△ ]]> Comparative Example 5 <![CDATA[56.28±5.24 *△ ]]> Comparative Example 6 <![CDATA[59.74±5.86 *△ ]]> Comparative Example 7 <![CDATA[57.14±5.78 *△ ]]> Comparative Example 8 <![CDATA[58.53±5.66 *△ ]]> Comparative Example 9 <![CDATA[52.31±5.06 *△ ]]> Note: Compared with the blank group: P < 0.05 is indicated by "*"; compared with Example 3: P < 0.05 is indicated by "△".

[0053] Compared with Example 3, Comparative Examples 1-4 do not add one of Artemisia capillaris extract, L-rhamnose, soluble proteoglycan and succinoglycan, respectively, and the preparation process of Artemisia capillaris extract in Comparative Examples 5-9 is different from the preparation process of Artemisia capillaris extract in Example 3; among them, Comparative Examples 5-6 respectively use ethanol solutions with different volume fractions in preparation step B, Comparative Examples 7-8 respectively use different pressures for ultra-high pressure treatment in preparation step B, and Comparative Example 9 uses long-term room temperature immersion instead of the freezing treatment step and the ultra-high pressure treatment step.

[0054] From the hyaluronidase inhibition rate and cell migration rate data of Examples 1-3 and Comparative Examples 1-4 in Tables 2-3, it can be seen that Examples 1-3, in which the four components of Artemisia capillaris extract, L-rhamnose, soluble proteoglycan and succinoglycan are compounded, have higher hyaluronidase inhibition rate and cell migration rate, and the four components of Artemisia capillaris extract, L-rhamnose, soluble proteoglycan and succinoglycan have a synergistic effect, which enhances the moisturizing and repairing ability of the composition.

[0055] Combining the data of Example 3 and Comparative Examples 5-9 in Tables 2-3, it can be seen that the preparation process of Artemisia capillaris extract will also significantly affect the extraction efficiency and effect of Artemisia capillaris extract, thereby affecting the hyaluronidase inhibition rate and cell migration rate of the composition, that is, affecting the moisturizing and repairing effect of the composition.

[0056] In summary, the preparation method of the Artemisia capillaris extract provided by the present invention adopts freezing, ultra-high pressure treatment and flash extraction, which has the advantages of improving extraction efficiency and avoiding degradation of active ingredients, and is easy to operate, low cost, and can retain the effective ingredients to the maximum extent; the high-efficiency moisturizing and repairing composition provided by the present invention is composed of a compound of Artemisia capillaris extract, L-rhamnose, soluble proteoglycan and succinoglycan, has good moisturizing and repairing effects, is mild and non-irritating, and has broad application prospects.

[0057] Test Example 3 Cytotoxicity Test Test substance: the moisturizing and repairing composition prepared in Examples 1-3; The test specifically includes the following steps: 3.1 Take a stable human immortalized epidermal cell line (HaCaT) and adjust the cell concentration to 1×10 5 cells / mL, 100 μL per well was inoculated into a 96-well cell culture plate (except the outermost well and the blank control well); 200 μL PBS was added to the outermost well of the cell culture plate to reduce evaporation of the culture medium; the 96-well cell culture plate was placed in a carbon dioxide incubator at 37°C ± 1°C, CO2 concentration of 5.0% ± 1%, and cultured until the cells reached more than 90% confluency; 3.2 Dilute the test substance to a concentration of 10 mg / L with PBS for later use. Set up a blank control group (use PBS instead of the test substance) and a test substance group, with 100 μL per well. Set up 6 replicates for each group, and present the data as the average value. Start the time from the beginning of the addition of the test substance (test substance group) and PBS (blank control), and keep the time interval between each two columns of sample additions consistent to ensure that the intervention time for each group is 5 minutes. 3.3 After 5 minutes of intervention, remove the test substances in turn and add PBS to each well to wash the test substances twice. When using a vacuum aspirator or a pipette to remove the liquid in the wells, be careful to avoid removing viable cells and affecting the experimental results. 3.4 Add 100 μL of DMEM medium and 10 μL of CCK-8 solution to each well and incubate in an incubator for about 2 hours; 3.5 Measure the absorbance (OD) at 450 nm and calculate the cell viability according to the following formula: ; The test results are shown in Table 4; Table 4 Cell survival rate data Group Cell survival rate (%) Example 1 96.3 Example 2 94.6 Example 3 98.1 The cell survival rates of Examples 1-3 were all higher than 94%, indicating that the high-efficiency moisturizing and repairing compositions provided by the present invention had no toxic effects on cells and had high safety.

[0058] The above description is merely a preferred composition of the present invention and does not constitute any form of limitation to the present invention. Although the present invention has been disclosed as a preferred composition as above, it is not intended to limit the present invention. Any person skilled in the art can make some changes or modifications to equivalent compositions using the technical contents disclosed above without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above compositions based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A highly effective moisturizing and repairing composition, characterized in that: The composition comprises the following components by weight: 14-18 parts of Artemisia capillaris extract, 7-11 parts of L-rhamnose, 9-13 parts of soluble proteoglycan, and 5-9 parts of succinoglycan; The preparation method of the Artemisia capillaris extract comprises the following steps: A. Take dried Artemisia capillaris, crush it, and pass it through a 30-mesh sieve to obtain Artemisia capillaris powder; B. adding ethanol solution to the Artemisia capillaris powder, mixing uniformly, freezing, and then performing ultrahigh pressure treatment to obtain a mixture; C. placing the mixture in a flash extractor for flash extraction to obtain an extract; D. Centrifuge the extract, take the supernatant and rotary evaporate until it is free of alcohol to obtain the Artemisia capillaris extract.

2. The composition according to claim 1, wherein The composition comprises the following components by weight: 16 parts of Artemisia capillaris extract, 9 parts of L-rhamnose, 11 parts of soluble proteoglycan, and 7 parts of succinoglycan.

3. The composition according to claim 1, wherein The volume fraction of the ethanol solution in step B is 55-65%, and the material-liquid ratio of Artemisia capillaris powder to the ethanol solution is 1 g:12-17 mL.

4. The composition according to claim 1, wherein The freezing treatment in step B is performed at a temperature of -20 to -15°C and for a time of 22 to 32 minutes.

5. The composition according to claim 1, wherein The pressure of the ultrahigh pressure treatment in step B is 250-350 MPa, the number of ultrahigh pressure treatments is 2-4 times, and the time of each ultrahigh pressure treatment is 40-60 seconds.

6. The composition according to claim 1, wherein In step C, the voltage of the flash extraction is 90-110 V, the number of flash extractions is 2-4 times, the time of each flash extraction is 30-45 seconds, and the speed of the flash extraction is 4000-5000 rpm.

7. The method for preparing the composition according to any one of claims 1 to 6, wherein: The preparation method comprises the following steps: weighing each component according to the formula amount, mixing and homogenizing, and obtaining the composition.

8. Use of the composition according to any one of claims 1 to 6 or the composition prepared by the preparation method according to claim 7 in the preparation of high-efficiency moisturizing and repairing products.

9. The use of the composition according to claim 8, wherein The highly effective moisturizing and repairing product is an essence, gel, lotion, facial mask or facial cream.