Traditional Chinese medicine composition capable of preventing and treating duck plague, preparation method thereof and traditional Chinese medicine preparation

Abstract

The invention relates to the technical field of traditional Chinese medicine preparations, and particularly discloses a traditional Chinese medicine composition capable of preventing and treating duck plague, a traditional Chinese medicine preparation and a preparation method and application thereof. The traditional Chinese medicine composition capable of preventing and treating duck disease comprises the following raw material components in parts by mass: 5-15 parts of corydalis conspersa, 5-15 parts of garlic, 15-25 parts of wild buckwheat rhizome, 5-10 parts of Chinese pulsatilla root, 5-10 parts of dandelion, 5-10 parts of scutellaria baicalensis, 5-10 parts of fructus forsythiae, 1-5 parts of humifuse euphorbia herb and 1-5 parts of liquorice. The traditional Chinese medicine preparation is prepared by taking the traditional Chinese medicine composition and polysaccharide as main raw materials, the traditional Chinese medicine preparation can be used for rapidly and effectively preventing and treating the duck plague, the preparation method is simple and convenient, the cost is low, and the defects in the prior art are overcome.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicines, and in particular to a traditional Chinese medicine composition capable of preventing and treating duck plague, a preparation method thereof and a traditional Chinese medicine preparation. Background Art

[0002] Duck plague, also known as duck infectious serositis or duck septicemia, is a contagious disease caused by Riemerella anatipestifer. Characterized by fibrinous pericarditis, air sacculitis, perihepatitis, salpingitis, arthritis, and meningitis, the disease primarily affects ducklings under eight weeks old, with ducklings between three and four weeks of age being most susceptible. With a mortality rate as high as 75%, it is a major infectious disease causing significant losses to the duck farming industry.

[0003] At present, the main prevention and control measure for this disease is the use of antibiotic vaccines, but the frequent use of antibiotics can easily lead to drug resistance, poor treatment effects, meat residues and high prevention and control costs. Therefore, the development of a traditional Chinese medicine preparation for the prevention and treatment of duck plague can effectively overcome the problems of drug resistance and drug residues of chemical drugs, which has important practical significance for the development of the duck farming industry and human health.

[0004] There are reports on the use of traditional Chinese medicine to treat Riemerella anatipestifer. However, existing traditional Chinese medicine preparations for preventing and treating duck plague still have certain deficiencies in terms of efficacy, safety, and stability. Furthermore, some traditional Chinese medicine preparations have very complex raw material formulas and cumbersome preparation processes, resulting in increased production costs. Furthermore, some traditional Chinese medicine preparations lack significant efficacy and fail to quickly and effectively alleviate symptoms, resulting in significant economic losses. Therefore, the development of a traditional Chinese medicine preparation for preventing and treating duck plague that is effective, simple to prepare, and highly safe is of great practical significance. Summary of the Invention

[0005] In light of this, the present invention provides a traditional Chinese medicine composition, preparation method, and preparation for duck plague. The raw materials of the traditional Chinese medicine composition include Corydalis variegata, garlic, golden buckwheat, Pulsatilla chinensis, Taraxacum mongolicum, Scutellaria baicalensis, Forsythia suspensa, Euphorbia milii, and Licorice. Furthermore, the present invention prepares a traditional Chinese medicine preparation using the traditional Chinese medicine composition and a polysaccharide as the main raw materials. The traditional Chinese medicine preparation can quickly and effectively prevent and treat duck plague, and the preparation method is simple and low-cost, thus overcoming the shortcomings of the existing technology.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] A first aspect of the present invention provides a traditional Chinese medicine composition for preventing and treating duck plague, comprising the following raw material components in parts by weight: 5-15 parts of Corydalis davidiana, 5-15 parts of garlic, 15-25 parts of Fagopyrum scabra, 5-10 parts of Pulsatilla chinensis, 5-10 parts of Taraxacum mongolicum, 5-10 parts of Scutellaria baicalensis, 5-10 parts of Forsythia suspensa, 1-5 parts of Herba Euphorbiae ibis and 1-5 parts of Licorice.

[0008] Compared to the existing technology, the present invention uses Corydalis variegata, garlic, golden buckwheat, Pulsatilla chinensis, Taraxacum officinale, Scutellaria baicalensis, Forsythia suspensa, Herba Euphorbiae chinensis, and Licorice as raw materials to prepare a traditional Chinese medicine composition for preventing and treating duck plague. Corydalis variegata is bitter and cooling in nature, entering the liver and lung meridians, and has the effects of clearing heat and detoxifying, cooling blood and reducing swelling. It can effectively relieve diarrhea and depression caused by duck plague, and reduce mortality. The allicin in garlic has extremely strong antibacterial activity, binding to lipids in bacterial cell membranes, changing cell membrane permeability, causing leakage of cellular contents and promoting cell death. Allicin can also block DNA replication and protein synthesis of Riemerella anatipestifer, resulting in significant antibacterial effects. The dimeric procyanidin contained in golden buckwheat not only damages the cell membrane of Riemerella anatipestifer and inhibits cell wall synthesis, but also increases the phagocytic activity of duck macrophages and the transformation rate of T lymphocytes, enhancing nonspecific immunity. Furthermore, golden buckwheat effectively alleviates pathological damage such as fibrinous pericarditis and perihepatitis caused by duck plague by reducing inflammatory factors. The anemonin and triterpenoid saponins in Pulsatilla chinensis can disrupt the cell membrane structure of Riemerella anatipestifer, inhibiting bacterial protein synthesis and DNA replication. For diarrhea and bloody dysentery caused by duck plague, Pulsatilla chinensis improves intestinal function by astringing the intestinal mucosa, reducing bleeding and exudation.

[0009] Dandelion, rich in vitamin C and minerals, can alleviate infection-induced decreased feed intake and weight loss, promoting recovery in sick ducks. Dandelion also promotes urination, accelerating the removal of toxins and pathogenic metabolites from the ducks' bodies and alleviating edema caused by infection. Scutellaria baicalensis, bitter and cold in nature, enters the lung, gallbladder, spleen, stomach, large intestine, and small intestine meridians. It has the effects of clearing heat and dampness, purging fire and detoxifying, and stopping bleeding. For the high fever caused by duck plague, the active ingredients in Scutellaria baicalensis can help regulate the temperature regulation center in the hypothalamus of sick ducks, reduce capillary permeability, exert an antipyretic effect, and alleviate symptoms such as depression and loss of appetite. It also promotes bile secretion, lowers transaminases, and protects liver cells. Forsythia suspensa, bitter and slightly cold in nature, enters the lung, heart, and small intestine meridians. It contains active ingredients such as forsythiaphenol and forsythiaside, which have the effects of clearing heat and detoxifying, inhibiting bacteria and inflammation, and enhancing immunity. Herba Euphorbiae Herba is bitter, pungent, and neutral in nature. It enters the liver, stomach, and large intestine meridians. It has the effects of clearing heat and detoxifying, drying dampness and stopping dysentery, promoting blood circulation and dispersing blood stasis, and dispelling wind and promoting urination. Herba Euphorbiae Herbae contains quercetin and gallic acid, which have potent inhibitory effects against Riemerella anatipestifer and Escherichia coli, and is particularly effective against diarrhea and bloody dysentery caused by intestinal infections. Licorice root is sweet and neutral in nature. It enters the heart, lung, spleen, and stomach meridians. The glycyrrhizic acid it contains can enhance the antibacterial activity of other raw materials while reducing their bitter and cold properties, reducing irritation to the spleen and stomach of ducklings. Licorice polysaccharides promote T lymphocyte proliferation, elevate interferon levels, and enhance ducks' resistance to pathogens.

[0010] The invention uses Corydalis davidiana, garlic, golden buckwheat and Pulsatilla chinensis as monarch drugs, Herba dandelae and baicalensis as ministerial drugs, Fructus Forsythiae and Herba Lycopodii as adjuvant drugs, and Radix Glycyrrhizae as guiding drug. By utilizing the monarch-minister-assistant-adjuvant-guiding-guide relationship of the drugs, the traditional Chinese medicine composition is endowed with the performance of quickly and effectively preventing and treating duck plague. The traditional Chinese medicine composition has a small number of raw material types and a low preparation cost, and effectively makes up for the shortcomings of the existing traditional Chinese medicine for treating duck plague, such as high preparation cost, poor treatment effect and slow onset.

[0011] Preferably, the traditional Chinese medicine composition for preventing and treating duck plague comprises the following raw material components in parts by mass: 6-12 parts of Corydalis davidiana, 8-12 parts of garlic, 18-22 parts of Fagopyrum scabra, 6-9 parts of Pulsatilla chinensis, 6-9 parts of Taraxacum mongolicum, 6-9 parts of Scutellaria baicalensis, 6-9 parts of Forsythia suspensa, 2-4 parts of Herba Euphorbiae ibis and 2-4 parts of Licorice.

[0012] Further preferably, the traditional Chinese medicine composition for preventing and treating duck plague comprises the following raw material components in parts by mass: 10 parts of Corydalis davidiana, 10 parts of garlic, 20 parts of Fagopyrum scabra, 8 parts of Pulsatilla scabra, 8 parts of Taraxacum mongolicum, 8 parts of Scutellaria baicalensis, 7 parts of Forsythia suspensa, 3 parts of Herba Euphorbiae ibis and 3 parts of Licorice.

[0013] The second aspect of the present invention provides a method for preparing a Chinese medicine composition for preventing and treating duck plague, comprising the following steps:

[0014] Step 1, weighing Corydalis variegata, garlic, golden buckwheat and Pulsatilla chinensis according to the designed ratio, mixing, crushing, soaking in a deep eutectic solvent, and performing solid-liquid separation to obtain a first extract;

[0015] Step 2: Weigh the remaining raw material components according to the designed ratio, mix them evenly, and crush them to obtain a mixture; immerse the mixture in an alcohol solution, decoct at 70-90° C., separate the solid and the liquid, repeat the decoction 2-4 times, and combine the filtrates to obtain a second extract;

[0016] Step 3: Evenly mix the first extract and the second extract, and concentrate the mixture until the relative density of the liquid medicine is 1.10-1.20, thereby obtaining a Chinese medicinal composition capable of preventing and treating duck plague.

[0017] The present invention utilizes deep eutectic solvents to extract the principal drug components, enhancing the solubility and extraction efficiency of the principal drug's active ingredients. Due to their excellent penetration and diffusion capabilities, deep eutectic solvents enhance mass transfer while simplifying the process, shortening extraction time, and reducing costs and energy consumption. More importantly, deep eutectic solvents, made from natural substances, exhibit extremely low volatility and high stability, do not interfere with the drug's ingredients, and possess excellent biocompatibility and biodegradability, making them more environmentally friendly and safe.

[0018] Preferably, in step 1, the deep eutectic solvent is water and glycerol in a mass ratio of 3:1-5:1.

[0019] The present invention adopts water and glycerol as a deep eutectic solvent, the raw materials are cheap, the preparation is simple, and the compatibility with subsequent separation technology is strong, and it has broad application prospects in the modern extraction of traditional Chinese medicine.

[0020] Preferably, in step 1, the mass volume ratio of the total mass of the Corydalis davidiana, garlic, fagopyrum scabra and Pulsatilla chinensis to the deep eutectic solvent is 1 g:5 mL-1 g:10 mL.

[0021] Preferably, in step 1, the soaking time is 4h-6h.

[0022] Preferably, in step 2, the alcohol solution is an ethanol aqueous solution with a mass concentration of 50%-65%.

[0023] Preferably, in step 2, the mass volume ratio of the mixture to the alcohol solution is 1 g:10 mL-1 g:15 mL.

[0024] Preferably, in step 2, the decocting time is 1 h to 2 h.

[0025] The third aspect of the present invention provides a traditional Chinese medicine preparation for preventing and treating duck plague, comprising a traditional Chinese medicine composition for preventing and treating duck plague prepared by the preparation method of the traditional Chinese medicine composition for preventing and treating duck plague, a polysaccharide and a pharmaceutically acceptable excipient; wherein the polysaccharide is a mixture of astragalus polysaccharide and honeysuckle polysaccharide.

[0026] The traditional Chinese medicine preparation provided by the present invention uses a traditional Chinese medicine composition and polysaccharides as its main raw materials. The polysaccharide is a mixture of astragalus polysaccharide and honeysuckle polysaccharide. Astragalus polysaccharide strengthens the body's vital energy and enhances disease resistance, while honeysuckle polysaccharide eliminates heat toxins and directly inhibits pathogens. The two complement each other, treating both the symptoms and the root cause, and reducing the recurrence rate of duck plague. The traditional Chinese medicine composition and polysaccharide synergistically enhance antibacterial and anti-inflammatory properties, allowing for rapid and effective prevention and treatment of duck plague.

[0027] Preferably, in the traditional Chinese medicine preparation, the mass ratio of the traditional Chinese medicine composition capable of preventing and treating duck plague to the polysaccharide is 8:1-12:1.

[0028] Preferably, the polysaccharides are astragalus polysaccharide and honeysuckle polysaccharide in a mass ratio of 1:1-1:1.5.

[0029] Preferably, the pharmaceutically acceptable excipient is one or more of a diluent, a wetting agent, a binder, a disintegrant, a preservative, a filler, a lubricant, a surfactant or a penetration enhancer.

[0030] Preferably, the traditional Chinese medicine preparation is a granule, oral liquid, tablet, capsule or other pharmaceutically acceptable external dosage form.

[0031] In summary, the present invention provides a traditional Chinese medicine preparation that can effectively prevent and treat duck plague, the core of which is a traditional Chinese medicine composition composed of variegated corydalis, garlic, golden buckwheat, pulsatilla, dandelion, scutellaria, forsythia, herba rutabagas, and licorice, on this basis, a polysaccharide is compounded. The traditional Chinese medicine preparation synergistically exerts the effects of clearing heat and detoxifying, antibacterial and anti-inflammatory, cooling blood and stopping dysentery through multiple traditional Chinese medicines, and combined with the immune enhancement and mucosal repair effects of polysaccharides, can quickly inhibit Riemerella anatipestifer, relieve diarrhea, respiratory symptoms and fibrinous inflammation, and significantly reduce the morbidity and mortality of duck plague. In addition, the preparation process of the traditional Chinese medicine preparation is simple and the raw material cost is low. It avoids the problem of antibiotic resistance and achieves both symptomatic and root cause treatment, effectively making up for the shortcomings of existing prevention and control technologies, and provides a new solution for the green prevention and control of duck plague. DETAILED DESCRIPTION

[0032] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0033] Example 1

[0034] This embodiment provides a Chinese medicine composition capable of preventing and treating duck plague and a preparation method thereof, which specifically includes the following contents:

[0035] The traditional Chinese medicine composition capable of preventing and treating duck plague comprises the following raw material components in parts by weight: 10g of Corydalis davidiana, 10g of garlic, 20g of Fagopyrum scabra, 8g of Pulsatilla chinensis, 8g of Taraxacum mongolicum, 8g of Scutellaria baicalensis, 7g of Forsythia suspensa, 3g of Herba Euphorbiae chinensis and 3g of Licorice.

[0036] The preparation method of the Chinese medicinal composition capable of preventing and treating duck plague comprises the following steps:

[0037] Step 1: Weigh Corydalis variegata, garlic, golden buckwheat, and Pulsatilla chinensis according to the designed ratio, mix, crush, and soak in 320 mL of a mixed solvent of water and glycerol in a mass ratio of 4:1 for 5 hours, and separate the solid and liquid to obtain a first extract;

[0038] Step 2: Weigh the remaining raw material components according to the designed ratio, mix them evenly, and crush them to obtain a mixture; immerse the mixture in 350 mL of 60% ethanol solution, decoct at 80° C., separate the solid and liquid, repeat the decoction three times, and combine the filtrates to obtain a second extract;

[0039] Step 3: Evenly mix the first extract and the second extract, and concentrate the mixture until the relative density of the liquid medicine is 1.12, thereby obtaining a Chinese medicine composition capable of preventing and treating duck plague.

[0040] Example 2

[0041] This embodiment provides a Chinese medicine composition capable of preventing and treating duck plague and a preparation method thereof, which specifically includes the following contents:

[0042] The traditional Chinese medicine composition capable of preventing and treating duck plague comprises the following raw material components in parts by weight: 12g of Corydalis davidiana, 8g of garlic, 15g of Fagopyrum scabra, 7g of Pulsatilla chinensis, 6g of Taraxacum mongolicum, 9g of Scutellaria baicalensis, 7g of Forsythia suspensa, 4g of Herba Euphorbiae chinensis and 4g of Licorice.

[0043] The preparation method of the Chinese medicinal composition capable of preventing and treating duck plague comprises the following steps:

[0044] Step 1: Weigh Corydalis variegata, garlic, golden buckwheat, and Pulsatilla chinensis according to the designed ratio, mix, crush, and soak in 350 mL of a mixed solvent of water and glycerol in a mass ratio of 5:1 for 5 hours, and separate the solid and liquid to obtain a first extract;

[0045] Step 2: Weigh the remaining raw material components according to the designed ratio, mix them evenly, and crush them to obtain a mixture; immerse the mixture in 350 mL of 60% ethanol solution, decoct at 80° C., separate the solid and liquid, repeat the decoction three times, and combine the filtrates to obtain a second extract;

[0046] Step 3: Evenly mix the first extract and the second extract, and concentrate the mixture until the relative density of the liquid medicine is 1.10, thereby obtaining a Chinese medicine composition capable of preventing and treating duck plague.

[0047] Example 3

[0048] This embodiment provides a Chinese medicine composition capable of preventing and treating duck plague and a preparation method thereof, which specifically includes the following contents:

[0049] The traditional Chinese medicine composition capable of preventing and treating duck plague comprises the following raw material components in parts by weight: 8g of Corydalis davidiana, 12g of garlic, 18g of Fagopyrum tiliaceum, 9g of Pulsatilla chinensis, 8g of Taraxacum mongolicum, 8g of Scutellaria baicalensis, 7g of Forsythia suspensa, 3g of Herba Euphorbiae chinensis and 3g of Licorice.

[0050] The preparation method of the Chinese medicinal composition capable of preventing and treating duck plague comprises the following steps:

[0051] Step 1: Weigh Corydalis variegata, garlic, golden buckwheat, and Pulsatilla chinensis according to the designed ratio, mix, crush, and soak in 300 mL of a mixed solvent of water and glycerol in a mass ratio of 3:1 for 5 hours, and separate the solid and liquid to obtain a first extract;

[0052] Step 2: Weigh the remaining raw material components according to the designed ratio, mix them evenly, and crush them to obtain a mixture; immerse the mixture in 350 mL of 60% ethanol solution, decoct at 90° C., separate the solid and liquid, repeat the decoction 4 times, and combine the filtrates to obtain a second extract;

[0053] Step 3: Evenly mix the first extract and the second extract, and concentrate the mixture until the relative density of the liquid medicine is 1.11, thereby obtaining a Chinese medicine composition capable of preventing and treating duck plague.

[0054] Example 4

[0055] This embodiment provides a Chinese medicine composition capable of preventing and treating duck plague and a preparation method thereof, which specifically includes the following contents:

[0056] The traditional Chinese medicine composition capable of preventing and treating duck plague comprises the following raw material components in parts by weight: 7g of Corydalis davidiana, 11g of garlic, 22g of Fagopyrum tiliaceum, 8g of Pulsatilla chinensis, 8g of Taraxacum mongolicum, 8g of Scutellaria baicalensis, 7g of Forsythia suspensa, 3g of Herba Euphorbiae chinensis and 3g of Licorice.

[0057] The preparation method of the Chinese medicinal composition capable of preventing and treating duck plague comprises the following steps:

[0058] Step 1: Weigh Corydalis variegata, garlic, golden buckwheat, and Pulsatilla chinensis according to the designed ratio, mix, crush, and soak in 300 mL of a mixed solvent of water and glycerol in a mass ratio of 4:1 for 5 hours, and separate the solid and liquid to obtain a first extract;

[0059] Step 2: Weigh the remaining raw material components according to the designed ratio, mix them evenly, and crush them to obtain a mixture; immerse the mixture in 320 mL of 60% ethanol solution, decoct at 80° C., separate the solid and liquid, repeat the decoction three times, and combine the filtrates to obtain a second extract;

[0060] Step 3: Evenly mix the first extract and the second extract, and concentrate the mixture until the relative density of the liquid medicine is 1.12, thereby obtaining a Chinese medicine composition capable of preventing and treating duck plague.

[0061] Example 5

[0062] This embodiment provides a Chinese medicine composition capable of preventing and treating duck plague and a preparation method thereof, which specifically includes the following contents:

[0063] The traditional Chinese medicine composition capable of preventing and treating duck plague comprises the following raw material components in parts by weight: 12g of Corydalis davidiana, 11g of garlic, 18g of Fagopyrum scabra, 6g of Pulsatilla chinensis, 8g of Taraxacum mongolicum, 9g of Scutellaria baicalensis, 9g of Forsythia suspensa, 3g of Herba Euphorbiae chinensis and 3g of Licorice.

[0064] The preparation method of the Chinese medicinal composition capable of preventing and treating duck plague comprises the following steps:

[0065] Step 1: Weigh Corydalis variegata, garlic, golden buckwheat, and Pulsatilla chinensis according to the designed ratio, mix, crush, and soak in 350 mL of a mixed solvent of water and glycerol in a mass ratio of 4:1 for 5 hours, and separate the solid and liquid to obtain a first extract;

[0066] Step 2: Weigh the remaining raw material components according to the designed ratio, mix them evenly, and crush them to obtain a mixture; immerse the mixture in 380 mL of 60% ethanol solution, decoct at 80° C., separate the solid and liquid, repeat the decoction three times, and combine the filtrates to obtain a second extract;

[0067] Step 3: Evenly mix the first extract and the second extract, and concentrate until the relative density of the liquid medicine is 1.14, thereby obtaining a traditional Chinese medicine composition capable of preventing and treating duck plague.

[0068] Comparative Example 1

[0069] This comparative example provides a Chinese medicine composition for preventing and treating duck plague and a preparation method thereof. The difference from Example 1 is that the Corydalis davidiana is replaced with an equal amount of Houttuynia cordata. The other ingredients and preparation process remain unchanged and will not be repeated here.

[0070] Comparative Example 2

[0071] This comparative example provides a traditional Chinese medicine composition for preventing and treating duck plague and a preparation method thereof. The difference from Example 1 is that garlic is replaced with an equal amount of Fraxinus officinalis. The other ingredients and preparation process remain unchanged and will not be described again here.

[0072] Comparative Example 3

[0073] This comparative example provides a traditional Chinese medicine composition for preventing and treating duck plague and a preparation method thereof. The difference from Example 1 is that golden buckwheat is replaced by an equal amount of lithospermum parkii, and the other ingredients and preparation process remain unchanged and will not be repeated here.

[0074] Comparative Example 4

[0075] This comparative example provides a traditional Chinese medicine composition for preventing and treating duck plague and a preparation method thereof. The difference from Example 1 is that Pulsatilla scutellaria is replaced with an equal amount of Sophora flavescens, and the other ingredients and preparation process remain unchanged and will not be repeated here.

[0076] Test Example 1

[0077] This test example provides a Chinese medicine granule for preventing and treating duck plague, which specifically includes the following contents:

[0078] 200 g of the traditional Chinese medicine composition for preventing and treating duck plague provided in each embodiment or comparative example, 20 g of a mixture of astragalus polysaccharide and honeysuckle polysaccharide in a mass ratio of 1:1.2, 20 g of microcrystalline cellulose, and 30 g of a 10% polyvinyl pyrrolidone solution were mixed uniformly, and the mixture was granulated using a fluidized bed wet method with a main fan temperature of 70 ° C, a fan frequency of 15 Hz, an infusion pump speed of 8 mL / min, and an atomization pressure of 1.8 bar. The mixture was mixed and granulated, and sieved to obtain traditional Chinese medicine granules for preventing and treating duck plague.

[0079] Other Chinese medicine preparations were prepared according to conventional preparation methods and will not be described in detail here.

[0080] In order to further demonstrate the technical effect of the present invention, the present invention conducted the following tests on the Chinese medicine spray prepared using Test Example 1:

[0081] (1) Mouse model experiments

[0082] Model construction: Each mouse in each group was subcutaneously injected with Riemerella anatipestifer liquid (concentration 3.5×10 8 CFU / mL) 0.1 mL, and mice in the blank control group were injected with 0.1 mL of sterile saline.

[0083] Healthy mice weighing 18-22 g, half male and half female, were randomly divided into 8 groups, 20 in each group, including a blank control group (not infected with Riemerella anatipestifer and not administered); a model control group (infected with Riemerella anatipestifer but not administered); an Example 1 group (a Chinese medicine granule for preventing and treating duck plague prepared using the Chinese medicine composition provided in Example 1); a comparative example 1 group (a Chinese medicine granule for preventing and treating duck plague prepared using the Chinese medicine composition provided in Comparative Example 1); a comparative example 2 group (a Chinese medicine group 2 group (a Chinese medicine group 3 group) Comparative Example 3 (a Chinese medicine granule for preventing and treating duck plague prepared using the Chinese medicine composition provided in Comparative Example 3); Comparative Example 4 (a Chinese medicine granule for preventing and treating duck plague prepared using the Chinese medicine composition provided in Comparative Example 4); a positive control group (commercially available florfenicol powder); dosage: the Chinese medicine granules and commercially available florfenicol powder were dissolved in physiological saline, 200 mg / kg by weight of mice, and administered orally twice a day for 7 consecutive days. The statistical test results are shown in Table 1.

[0084] All mice in the model control group died within 12 hours, indicating that the infection model of Riemerella anatipestifer in mice was successfully established.

[0085] Efficacy evaluation criteria: Cured: Symptoms completely disappear, mental state, food intake and water intake return to normal, and there is no recurrence of the disease; Effective: Symptoms are significantly improved or improved, mental state, food intake and water intake basically return to normal; Ineffective: Symptoms do not improve, and there is even a tendency to worsen, leading to death.

[0086] Table 1 Results of mouse model experiments

[0087]

[0088]

[0089] As can be seen from Table 1, the Chinese medicine preparation provided by Example 1 of the present invention has a good therapeutic effect on mice artificially infected with Riemerella anatipestifer, and its therapeutic effect is comparable to that of the positive control group, but the pure Chinese medicine preparation does not have the disadvantages of drug resistance and drug residues of chemical drugs.

[0090] (2) Acute toxicology test

[0091] Healthy mice weighing 18-22 g, half male and half female, were randomly divided into 5 groups, 20 in each group, namely a blank control group (not infected with Riemerella anatipestifer and not administered); a model control group (infected with Riemerella anatipestifer but not administered); a low-dose group (a Chinese medicine granule for preventing and treating duck plague prepared using the Chinese medicine composition provided in Example 1); a medium-dose group (a Chinese medicine granule for preventing and treating duck plague prepared using the Chinese medicine composition provided in Comparative Example 1); and a high-dose group (a Chinese medicine granule for preventing and treating duck plague prepared using the Chinese medicine composition provided in Comparative Example 2); the mice were gavage-administered twice a day for 7 consecutive days. The statistical test results are shown in Table 2.

[0092] Table 2 Acute toxicity test results

[0093]

[0094]

[0095] As can be seen from Table 2, the medium-dose group had the highest cure rate and the lowest mortality rate, and had a good therapeutic effect on artificial infection of mice with Riemerella anatipestifer.

[0096] (3) Animal experiments on Riemerella anatipestifer

[0097] Case Selection Criteria: Based on the clinical symptoms of Riemerella anatipestifer and incorporating Traditional Chinese Veterinary theory, Riemerella anatipestifer falls into the category of "febrile diseases and acute convulsions," affecting the heart, liver, and lungs, with heat and excess symptoms at the core. It is caused by internal invasion of pathogenic toxins, with wind-heat invading the lungs and then spreading to the heart and liver, leading to acute convulsions, followed by excessive phlegm and heat. Excessive wind generates phlegm, which in turn generates convulsions, leading to flesh rot and blood loss, resulting in carbuncles and suppuration. Alternatively, heat damages the blood vessels, preventing the elimination of toxins due to insufficient body fluids, exacerbating toxic blood and septicemia. Clinical manifestations include depression, decreased food intake, unsteady standing, general tremors, a tilted head and neck, a stargazing posture, and legs stretched out in an opisthotonic position. Coughing, nasal wagging, open-mouthed breathing, loose yellow-green stools, and gradual collapse and convulsions leading to death.

[0098] The duck with the death disease is subjected to pathological dissection, and it can be seen that the heart and pericardium have effusion, the capsule is thickened, the epicardium has fibrinous exudate, the air sac is turbid and thickened, the surface is covered with fibrinous exudate and is grayish white or grayish yellow, the liver is swollen and fragile, is earthy yellow or brown red, the capsule is thickened, is opaque and is yellowish white, the spleen is swollen and is red and gray mottled, the gallbladder is filled. In some chronic infections, fibrinous meningitis or meningeal hyperemia, edema or punctate hemorrhage and salpingitis can be seen, and the duck is diagnosed as having duck rimer's bacillus disease.

[0099] Test animals: according to the case selection standard, the diseased ducks were selected from a duck farm in Xinji, Hebei, half male and half female, the body weight was in the range of 1.2-1.4 kg, and were randomly divided into 8 groups, 100 ducks in each group, respectively blank control group (not infected with duck rimer's bacillus and not given drug); model control group (infected with duck rimer's bacillus but not given drug); example 1 group (duck epidemic prevention and treatment traditional Chinese medicine granules prepared by using the traditional Chinese medicine composition provided in example 1); comparative example 1 group (duck epidemic prevention and treatment traditional Chinese medicine granules prepared by using the traditional Chinese medicine composition provided in comparative example 1); comparative example 2 group (duck epidemic prevention and treatment traditional Chinese medicine granules prepared by using the traditional Chinese medicine composition provided in comparative example 2); comparative example 3 group (duck epidemic prevention and treatment traditional Chinese medicine granules prepared by using the traditional Chinese medicine composition provided in comparative example 3); comparative example 4 group (duck epidemic prevention and treatment traditional Chinese medicine granules prepared by using the traditional Chinese medicine composition provided in comparative example 4); positive control group (marketed florfenicol powder); dosage: the traditional Chinese medicine granules and the marketed florfenicol powder were dissolved in physiological saline, and the dosage was 200 mg / kg according to the body weight of the duck, and was administered by gavage, twice a day, in the morning and in the evening, for 7 days, and the test results were counted, as shown in table 3.

[0100] Therapeutic evaluation standard: cured: symptoms completely disappeared, mental state, food intake and water intake returned to normal, and there was no recurrence; effective: symptoms were obviously improved or improved, mental state, food intake and water intake basically returned to normal; ineffective: symptoms did not improve, and even had a tendency to aggravate, to death.

[0101] Table 3 animal experiment test results

[0102]

[0103] According to table 3, it can be seen that the duck epidemic prevention and treatment traditional Chinese medicine preparation provided by the application has good therapeutic effect, and the therapeutic effect is equivalent to that of the positive control group.

[0104] The above only describes the preferred embodiments of the application, and is not intended to limit the application, and any modification, equivalent replacement or improvement made within the spirit and principle of the application shall be included in the protection scope of the application.

Claims

1. A Chinese medicine composition for preventing and treating duck plague, characterized in that: The invention comprises the following raw material components in parts by weight: 5-15 parts of Corydalis variegata, 5-15 parts of garlic, 15-25 parts of Fagopyrum scabra, 5-10 parts of Pulsatilla chinensis, 5-10 parts of Taraxacum mongolicum, 5-10 parts of Scutellaria baicalensis, 5-10 parts of Forsythia suspensa, 1-5 parts of Herba Euphorbiae chinensis and 1-5 parts of Licorice.

2. The Chinese medicinal composition for preventing and treating duck plague according to claim 1, wherein: The invention comprises the following raw material components in parts by weight: 6-12 parts of Corydalis davidiana, 8-12 parts of garlic, 18-22 parts of golden buckwheat, 6-9 parts of Pulsatilla chinensis, 6-9 parts of Taraxacum mongolicum, 6-9 parts of Scutellaria baicalensis, 6-9 parts of Forsythia suspensa, 2-4 parts of Herba Euphorbiae chinensis and 2-4 parts of Licorice.

3. The Chinese medicinal composition for preventing and treating duck plague according to claim 1 or 2, characterized in that: The invention comprises the following raw material components in parts by weight: 10 parts of Corydalis davidiana, 10 parts of garlic, 20 parts of golden buckwheat, 8 parts of Pulsatilla scabra, 8 parts of Taraxacum mongolicum, 8 parts of Scutellaria baicalensis, 7 parts of Forsythia suspensa, 3 parts of Herba Euphorbiae chinensis and 3 parts of Licorice.

4. A method for preparing a Chinese medicinal composition for preventing and treating duck plague according to any one of claims 1 to 3, characterized in that: The steps include: Step 1, weighing Corydalis variegata, garlic, golden buckwheat and Pulsatilla chinensis according to the designed ratio, mixing, crushing, soaking in a deep eutectic solvent, and performing solid-liquid separation to obtain a first extract; Step 2: Weigh the remaining raw material components according to the designed ratio, mix them evenly, and crush them to obtain a mixture; immerse the mixture in an alcohol solution, decoct at 70-90° C., separate the solid and the liquid, repeat the decoction 2-4 times, and combine the filtrates to obtain a second extract; Step 3: Evenly mix the first extract and the second extract, and concentrate the mixture until the relative density of the liquid medicine is 1.10-1.20, thereby obtaining a Chinese medicine composition capable of preventing and treating duck plague.

5. The method for preparing the Chinese medicinal composition for preventing and treating duck plague according to claim 4, wherein: In step 1, the deep eutectic solvent is water and glycerol in a mass ratio of 3:1-5:1; and / or In step 1, the mass volume ratio of the total mass of the Corydalis variegata, garlic, golden buckwheat and Pulsatilla chinensis to the deep eutectic solvent is 1g:5mL-1g:7mL; and / or In step 1, the soaking time is 4h-6h.

6. The method for preparing the Chinese medicinal composition for preventing and treating duck plague according to claim 4, wherein: In step 2, the alcohol solution is an ethanol aqueous solution with a mass concentration of 50%-65%; and / or In step 2, the mass volume ratio of the mixture to the alcohol solution is 1g:10mL-1g:15mL; and / or In step 2, the decocting time is 1h-2h.

7. A Chinese medicinal preparation for preventing and treating duck plague, characterized in that: The invention comprises a Chinese medicinal composition for preventing and treating duck plague prepared by the preparation method of the Chinese medicinal composition for preventing and treating duck plague according to any one of claims 4 to 6, polysaccharide and pharmaceutically acceptable excipients; wherein the polysaccharide is a mixture of astragalus polysaccharide and honeysuckle polysaccharide.

8. The Chinese medicinal preparation for preventing and treating duck plague according to claim 7, wherein: In the traditional Chinese medicine preparation, the mass ratio of the traditional Chinese medicine composition capable of preventing and treating duck plague to the polysaccharide is 8:1-12:1; and / or The polysaccharides are astragalus polysaccharide and honeysuckle polysaccharide in a mass ratio of 1:1-1:1.

5.

9. The Chinese medicinal preparation for preventing and treating duck plague according to claim 7, wherein: The pharmaceutically acceptable excipient is one or more of a diluent, a wetting agent, a binder, a disintegrant, a preservative, a filler, a lubricant, a surfactant or a penetration enhancer.

10. The Chinese medicinal preparation for preventing and treating duck plague according to any one of claims 7 to 9, characterized in that: The traditional Chinese medicine preparation is a granule, oral liquid, tablet, capsule or other pharmaceutically acceptable external dosage form.

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