Laboratory water bath kettle

Abstract

The invention discloses a laboratory water bath kettle, and relates to the technical field of water bath kettles. According to the laboratory water bath kettle, a jacking column stably performs reciprocating linear movement, and an acute angle is formed between the impact angle of the jacking column and the end face of a test tube rack, so that the stress direction of the test tube rack is changed into left-right and front-back shaking, and a single impact force is decomposed into a horizontal component force and a vertical component force; the test tube rack is driven to swing in the left-right direction and to slightly vibrate in the front-back direction, so that the motion of the test tube rack is superposed, a compound motion track is realized through single-shaft impact, an artificial three-dimensional shaking action is simulated, a spiral rising vortex is formed, the bottom, the edge and the liquid level area of the test tube are covered, and a mixing dead angle caused by standing or single-shaft oscillation is avoided; the acceleration direction of the composite motion changes continuously, periodic one-way stress concentration of uniaxial oscillation is avoided, fragile cells are protected, the thickness of a thermal boundary layer near the test tube wall is reduced through the three-dimensional motion, the cryopreservation tube is heated more uniformly, the thawing time is shortened, and the cell survival rate and the functional integrity are improved.

Description

Technical Field

[0001] The invention relates to the technical field of water bath pots, in particular to a laboratory water bath pot. Background Art

[0002] A laboratory water bath is a device that precisely controls the temperature of samples in test tubes, centrifuge tubes, or culture bottles by heating liquid at a constant temperature. During the cell recovery process, it quickly thaws frozen cells and quickly warms cells frozen in -196°C liquid nitrogen or a -80°C refrigerator to 37°C to prevent ice crystals from damaging the cell membrane, ensuring that cells are at the optimal temperature during the recovery process and improving survival rates. Through the shaking or stirring function, the cell suspension is evenly distributed to avoid the toxic effects of local high concentrations of DMSO on cells.

[0003] Existing laboratory water baths have a single structure for shaking test tubes. Traditional horizontal or vertical oscillations only apply periodic force in a single direction, resulting in directional shear flow in the liquid in the test tube. Cells are subjected to repetitive mechanical stress, which causes mechanical damage to the cells in the test tube. The shear force causes the phospholipid bilayer of the cell membrane to break, and the influx of calcium ions activates the apoptosis pathway. Suspended cells gather at the edge of the test tube due to changes in density gradients, the frequency of local collisions increases, and uneven heating causes large local temperature differences in the test tube wall. Combined with mechanical vibration, the test tube can easily burst. In addition, the lid of the test tube is not tightly sealed during transportation, allowing liquid nitrogen to enter. In this case, the test tube is prone to explosion as long as it is heated. There is no protective structure, which can cause personal injury to the operator. Summary of the Invention

[0004] Technical problems solved

[0005] Aiming at the problem that the existing laboratory water bath has a single shaking structure and the single shaking easily causes mechanical damage to cells in the test tube, the present invention provides a laboratory water bath.

[0006] Technical Solution

[0007] In order to achieve the purpose that the shaking structure of a laboratory water bath can simulate the artificial three-dimensional shaking action of a test tube, with one end of the test tube fixed and the other end shaking in three dimensions, the present invention is achieved through the following technical solutions: a laboratory water bath, comprising a water bath shell, a hydraulic press is installed on the outer surface of the water bath shell, a connecting block 1 is installed on one end surface of the output end of the hydraulic press, a shaking table is installed on the outer surface of the connecting block 1, a slot is provided inside the shaking table, a test tube rack is movably installed on the inner wall of the slot, and multiple specifications of fixing components are evenly installed inside the test tube rack, and the multiple specifications of fixing components are convenient for fixing test tubes of different sizes for limited fixation;

[0008] The outer surface of the water bath shell is provided with a stable lifting assembly, and the outer surface of the stable lifting assembly is provided with a main water bath cover, and the outer surface of the water bath shell is provided with a vibration power assembly, and the outer surface of the vibration power assembly is provided with a shaking assembly, and the shaking assembly comprises a sealing sleeve and two transmission shafts, and the sealing sleeve is installed on the outer surface of the shaking platform, and the two transmission shafts are movably installed in the interior of the sealing sleeve block, one end surface of the transmission shaft is provided with a turbine, and the outer surface of the other transmission shaft is provided with a column. The outer surface of the column is provided with a reciprocating groove, and a reciprocating clamping plate is movably installed at the inner wall of the reciprocating groove, and a top column is installed on one end surface of the reciprocating clamping plate, and a clamping block 2 is installed on the outer surface of the top column, and a clamping slot 2 is provided inside the sealing sleeve block, and the outer surface of the clamping block 2 and the outer surface of the top column are both movably in contact with the inner wall of the clamping slot 2, and one end surface of the top column is movably in contact with the outer surface of the test tube rack, and the top column forms an acute angle with the end face of the test tube rack;

[0009] The shaking assembly causes the column to move back and forth at high speed to squeeze and vibrate the test tube rack, causing the test tube rack to shake regularly. The turbine drives the water flow to stir and perform heat exchange. The interior of the shaking table is evenly distributed with an active cavity.

[0010] Furthermore, the vibration power assembly includes a motor and a rotating shaft, the motor is installed on the outer surface of the water bath shell, the rotating shaft is installed on the outer surface of the motor output end, a card slot is opened inside the rotating shaft, a transmission shaft is movably installed inside the sealing sleeve, two card blocks are installed on the outer surface of the transmission shaft, and the outer surfaces of the two card blocks and the outer surface of the transmission shaft are both in movably contact with the inner wall of the card slot.

[0011] Furthermore, two main helical gears are installed on the outer surface of the transmission shaft 1, and two slave helical gears are installed on the outer surfaces of the two transmission shafts 2. The outer surfaces of the two main helical gears mesh with the outer surfaces of the two slave helical gears.

[0012] Furthermore, the stable lifting assembly includes two limiting slide rails and two guide columns, the two limiting slide rails are installed on the inner surface of the water bath shell, the two guide columns are movably installed inside the water bath shell, and the outer surfaces of the two guide columns are both installed with two connecting blocks 2, wherein the outer surfaces of two of the connecting blocks 2 are fixedly connected to the outer surface of the shaking table, and the outer surfaces of the other two connecting blocks 2 are fixedly connected to the outer surface of the main water bath cover, and the outer surfaces of the four connecting blocks 2 are all installed with limiting sliders, and the outer surfaces of the limiting sliders are movably contacted with the outer surfaces of the limiting slide rails.

[0013] Furthermore, hinges are evenly installed on the outer surface of the main water bath pot cover, and auxiliary water bath pot covers are commonly installed on the outer surfaces of several hinges. The bottom end surfaces of the auxiliary water bath pot covers and the bottom end surface of the main water bath pot cover are both in active contact with the top end surface of the water bath pot shell.

[0014] Furthermore, two handles are installed on the top surface of the test tube rack, the test tube rack is provided with an inclined surface, and test tube insertion holes are evenly opened inside the test tube rack. The multi-specification fixing component includes a large annular groove and a disc, the large annular groove is opened on the top surface of the test tube rack, the disc is movably installed inside the test tube rack, and a shift block is installed on the top surface of the disc, and the outer surface of the shift block is in movably contact with the inner wall of the large annular groove.

[0015] Furthermore, a progressive thread strip is movably installed on the top surface of the disc, and four card plates are movably installed inside the test tube rack. The bottom surfaces of the four card plates are all provided with progressive thread grooves, and the outer surfaces of the progressive thread strip are movably contacted with the inner walls of the progressive thread grooves. A movable cavity 2 is provided inside the four card plates, and a rubber block 2 is installed on one end surface of the four card plates.

[0016] Furthermore, stable limiting components are installed on the inner wall of the active cavity 1 and the inner wall of the active cavity 2, and the number of stable limiting components on the inner wall of the active cavity 1 is several.

[0017] Furthermore, the stable limiting assembly includes a fixed column and a telescopic cavity 1, the fixed column is movably installed on the inner wall of the movable cavity 1, a telescopic cavity 1 is opened inside the fixed column, a spring 1 is installed on the inner wall of the telescopic cavity 1, a telescopic column is installed on the outer surface of the spring 1, a rubber block 1 is commonly installed on the outer surfaces of several telescopic columns inside the shaking platform, a movable block is installed on the outer surface of the telescopic column inside the card plate, and the outer surface of the movable block is movably contacted with the inner wall of the movable cavity 2.

[0018] Furthermore, a telescopic cavity 2 is opened inside the movable block, and springs 2 are evenly installed on the inner wall of the telescopic cavity 2. Rubber blocks 3 are commonly installed on the outer surfaces of several springs 2, and the outer surfaces of the rubber blocks 3 are in movable contact with the inner wall of the telescopic cavity 2.

[0019] Beneficial effects

[0020] The present invention has the following beneficial effects:

[0021] (1) The laboratory water bath is able to move back and forth in a stable linear manner by means of a top column, and the impact angle of the top column forms an acute angle with the end face of the test tube rack, so that the direction of force on the test tube rack changes to left and right and front and back shaking, decomposing the single impact force into horizontal and vertical components, driving the test tube rack to swing in the left and right directions and driving the test tube rack to vibrate in the front and back directions, so that the test tube rack motion is superimposed, and a composite motion trajectory of "left and right swing + front and back axis oscillation" is achieved through uniaxial impact, simulating artificial three-dimensional shaking action, forming a spiral rising vortex, covering the bottom, edge and liquid surface area of ​​the test tube, avoiding the mixed dead angle caused by static or uniaxial oscillation, and the acceleration direction of the composite motion changes continuously, avoiding the periodic unidirectional stress concentration of uniaxial oscillation, protecting fragile cells, and the three-dimensional motion reduces the thickness of the thermal boundary layer near the test tube wall, shortens the thawing time, and improves cell survival rate and functional integrity.

[0022] (2) The laboratory water bath uses the second transmission shaft to stably drive the turbine to rotate. The turbine stirs the water inside the laboratory water bath, so that the water temperature outside the test tube undergoes heat exchange. The three-dimensional oscillation compound movement inside the test tube promotes uniform mixing of the cell suspension. The turbine stirring outside the test tube ensures uniform water bath temperature, forming a synergistic effect of "internal mixing and external uniformity", thereby shortening the cell recovery time. The rotation of the turbine forms an annular flow field, eliminating the cold and hot circulation dead zone of the traditional water bath.

[0023] (3) The laboratory water bath is shielded by the main water bath cover and the auxiliary water bath cover which are simultaneously moved to the top surface of the water bath shell. When the test tube cells burst during the recovery process, the main water bath cover and the auxiliary water bath cover will shield and protect the operator without causing personal injury, thus reducing damage to the experimental environment and other equipment.

[0024] (4) The laboratory water bath uses the deflection angle of the shift block to limit the outer end of the rubber block 2 to the outer side of the test tube. When the card plate moves, the movable block will squeeze the outer surface of the disc, so that the bottom end of the test tube is squeezed and fixed, and the upper end is only limited. The rubber block 2 and the rubber block 3 are made of hard rubber on one end surface, and the surface that fits the test tube is made of elastic rubber material, so that the test tube rack can fix test tubes of different sizes and test tubes of different conical shapes. The test tube rack can adjust the deflection angle of the shift block so that the card plate drives the rubber block 2 to move, thereby adapting to the fixing requirements of test tubes of different sizes. Whether it is a small or large test tube, it can achieve stable limiting. The upper end limiting and bottom end fixing method not only ensures the stability of the test tube in the test tube rack, but also avoids unnecessary pressure on the test tube caused by excessive fixing, which is beneficial to the cell recovery operation.

[0025] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0027] Figure 2 Schematic diagram of the internal structure of the present invention;

[0028] Figure 3 This is a schematic diagram of the internal structure of the present invention from another perspective;

[0029] Figure 4 This is a schematic diagram of the overall structure of the stable lifting assembly of the present invention;

[0030] Figure 5 Schematic diagram of the internal structure of the vibration power assembly of the present invention;

[0031] Figure 6 This is a schematic diagram of the internal structure of the shaking assembly of the present invention;

[0032] Figure 7 This is a schematic diagram of the overall structure of the stabilizing and limiting assembly of the present invention;

[0033] Figure 8 This is a schematic diagram of the overall structure of the test tube rack of the present invention;

[0034] Figure 9 This is a schematic diagram of the internal structure of the test tube rack of the present invention;

[0035] Figure 10 This is a schematic diagram of the internal structure of the multi-specification fixing assembly of the present invention;

[0036] Figure 11 This is a schematic diagram of the internal structure of the multi-specification fixing assembly of the present invention from another perspective.

[0037] Figure: 1. Water bath shell; 2. Hydraulic press; 3. Connecting block 1; 4. Shaking table; 5. Slot; 6. Test tube rack; 7. Test tube jack; 8. Limiting rail; 9. Limiting slider; 10. Connecting block 2; 11. Guide column; 12. Main water bath lid; 13. Hinge; 14. Auxiliary water bath lid; 15. Rubber block 2; 16. Rubber block 3; 17. Motor; 18. Rotating shaft; 19. Slot 1; 20. Block 1; 21. Transmission shaft 1; 22. Sealing sleeve; 23. Main bevel gear; 24. Slave bevel gear; 25. Transmission Axis 2; 26. Handle; 27. Inclined surface; 28. Movable chamber 1; 29. ​​Movable chamber 2; 30. Turbine; 31. Column; 32. Reciprocating groove; 33. Reciprocating clamping plate; 34. Top column; 35. Clamping block 2; 36. Clamping groove 2; 37. Fixed column; 38. Telescopic chamber 1; 39. Telescopic column; 40. Spring 1; 41. Dial block; 42. Rubber block 1; 43. Large annular groove; 44. Disc; 45. Progressive thread groove; 46. Clamping block; 47. Progressive thread strip; 48. Spring 2; 49. Movable block; 50. Telescopic chamber 2. DETAILED DESCRIPTION

[0038] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0039] In the description of the present invention, it should be understood that the terms "opening", "upper", "lower", "thickness", "top", "middle", "length", "inside", "around" and the like indicating orientation or positional relationship are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the components or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0040] See also Figures 1-11 The embodiment of the present invention provides a technical solution: a laboratory water bath, comprising a water bath shell 1, a hydraulic press 2 mounted on the outer surface of the water bath shell 1, a connecting block 3 mounted on one end surface of the output end of the hydraulic press 2, a shaking table 4 mounted on the outer surface of the connecting block 3, a slot 5 formed inside the shaking table 4, a test tube rack 6 movably mounted on the inner wall of the slot 5, multiple-specification fixing components evenly mounted inside the test tube rack 6, the multiple-specification fixing components conveniently fixing test tubes of different sizes for limited fixation;

[0041] The inner surface of the water bath shell 1 is installed with a stable lifting component, the outer surface of the stable lifting component is installed with the main water bath cover 12, the outer surface of the water bath shell 1 is installed with a vibration power component, the outer surface of the vibration power component is installed with a shaking component, the shaking component includes a sealing sleeve 22 and two transmission shafts 25, the sealing sleeve 22 is installed on the outer surface of the shaking table 4, the two transmission shafts 25 are movably installed inside the sealing sleeve 22, one end surface of the transmission shaft 25 is installed with a turbine 30, the outer side of the other transmission shaft 25 is installed with a turbine 30, and the outer side of the other transmission shaft 25 is installed with a turbine 30. A column 31 is mounted on the surface, and a reciprocating groove 32 is provided on the outer surface of the column 31. A reciprocating clamping plate 33 is movably mounted on the inner wall of the reciprocating groove 32. A top column 34 is mounted on one end surface of the reciprocating clamping plate 33. A second clamping block 35 is mounted on the outer surface of the top column 34. A second clamping groove 36 is provided inside the sealing sleeve 22. The outer surface of the second clamping block 35 and the outer surface of the top column 34 are both in movably contact with the inner wall of the second clamping groove 36. One end surface of the top column 34 is in movably contact with the outer surface of the test tube rack 6, and the top column 34 forms an acute angle with the end surface of the test tube rack 6.

[0042] The shaking assembly causes the column 31 to reciprocate at high speed to squeeze and vibrate the test tube rack 6, causing the test tube rack 6 to shake regularly. The turbine 30 drives the water flow to stir and perform heat exchange. The interior of the shaking table 4 is evenly distributed with an active cavity 28. The top column 34 hits the end face of the test tube rack 6 at an acute angle, decomposing the single impact force into horizontal and vertical components, driving the test tube rack 6 to swing left and right and drive the test tube rack 6 to vibrate slightly in the front and back directions, thereby superimposing the movement of the test tube rack 6. Through single-axis impact, a composite motion trajectory of "left and right swing + front and back axis oscillation" is achieved, simulating artificial three-dimensional shaking. The three-dimensional motion forms a spiral upward vortex, covering the bottom, edge and liquid surface area of ​​the test tube, avoiding the mixing dead corner caused by static or uniaxial oscillation. The acceleration direction of the composite motion changes continuously, avoiding the periodic unidirectional stress concentration of uniaxial oscillation, protecting fragile cells, and simulating the "shake-pause" rhythm of the artificial wrist. Pausing only requires controlling the motor 17 to stop working, and several springs 40 quickly stabilize the test tube rack 6, reducing the mechanical damage to the cell membrane caused by the rapid melting of ice crystals. The three-dimensional motion reduces the thickness of the thermal boundary layer near the test tube wall, shortens the thawing time, and improves the cell survival rate and functional integrity.

[0043] The vibration power assembly includes a motor 17 and a rotating shaft 18. The motor 17 is mounted on the outer surface of the water bath shell 1, and the rotating shaft 18 is mounted on the outer surface of the output end of the motor 17. A card slot 19 is provided inside the rotating shaft 18. A transmission shaft 21 is movably installed inside the sealing sleeve 22. Two card blocks 20 are installed on the outer surface of the transmission shaft 21. The outer surfaces of the two card blocks 20 and the outer surface of the transmission shaft 21 are both in active contact with the inner wall of the card slot 19. Two main bevel gears 23 are installed on the outer surface of the transmission shaft 21. The outer surfaces of the two transmission shafts 25 are both mounted with slave bevel gears 24. The outer surfaces of the two main bevel gears 23 correspond to the outer surfaces of the two slave bevel gears. The outer surfaces of the wheels 24 are meshed with each other, and the controller controls the motor 17 to work. The motor 17 drives the rotating shaft 18 to rotate, and the rotating shaft 18 drives the card slot 19 to rotate. The inner wall of the card slot 19 squeezes the outer sides of the two card blocks 20, so that the two card blocks 20 drive the transmission shaft 21 to rotate, and the transmission shaft 21 drives the two main bevel gears 23 to rotate. The outer sides of the slave bevel gears 24 corresponding to the two main bevel gears 23 are meshed with each other, and one of the main bevel gears 23 drives one of the slave bevel gears 24 to rotate, and the slave bevel gear 24 drives one of the transmission shafts 25 to rotate, and the transmission shaft 25 drives the column 31 to rotate.

[0044] The stable lifting assembly includes two limiting slide rails 8 and two guide columns 11. The two limiting slide rails 8 are installed on the inner surface of the water bath shell 1, and the two guide columns 11 are movably installed inside the water bath shell 1. The outer surfaces of the two guide columns 11 are both installed with two connecting blocks 2 10, wherein the outer surfaces of two connecting blocks 2 10 are fixedly connected to the outer surface of the shaking table 4, and the outer surfaces of the other two connecting blocks 2 10 are fixedly connected to the outer surface of the main water bath cover 12. The outer surfaces of the four connecting blocks 2 10 are all installed with limiting sliders 9, and the outer surfaces of the limiting sliders 9 are in movably contact with the outer surfaces of the limiting slide rails 8. The outer surface of the main water bath cover 12 is evenly installed with hinges 13, and the outer surfaces of several hinges 13 are jointly installed with auxiliary water bath covers 14. The auxiliary water bath covers 14 The bottom surface of the main water bath lid 12 and the bottom surface of the main water bath lid 12 are both in active contact with the top surface of the water bath shell 1. The main water bath lid 12 and the auxiliary water bath lid 14 are also moved to the top surface of the water bath shell 1 synchronously, thereby shielding the water bath shell 1, so that the test tube cells will burst during the recovery process. The shielding protection of the main water bath lid 12 and the auxiliary water bath lid 14 will not cause personal injury to the operator, and reduce damage to the experimental environment and other equipment. The hydraulic press 2 achieves precise speed and position control through the controller to ensure that the shaking table 4 and the main water bath lid 12 and the auxiliary water bath lid 14 are lifted and lowered smoothly. The guiding function of the guide column 11 ensures straightness and stability during the lifting process, and effectively prevents mechanical failure or safety accidents caused by deviation or shaking.

[0045] The top surface of the test tube rack 6 is provided with two handles 26, the test tube rack 6 is provided with an inclined surface 27, the interior of the test tube rack 6 is evenly provided with test tube jacks 7, and the multi-specification fixing assembly includes a large annular groove 43 and a disc 44. The large annular groove 43 is provided on the top surface of the test tube rack 6, and the disc 44 is movably installed inside the test tube rack 6. A dial block 41 is installed on the top surface of the disc 44. The outer surface of the dial block 41 is in active contact with the inner wall of the large annular groove 43. A progressive thread strip 47 is movably installed on the top surface of the disc 44. Four card plates 46 are movably installed inside the test tube rack 6. The bottom surfaces of the four card plates 46 are all opened. There is a progressive thread groove 45, the outer surface of the progressive thread strip 47 is in active contact with the inner wall of the progressive thread groove 45, the interior of the four card plates 46 are all provided with a movable cavity 29, and one end surface of the four card plates 46 are all installed with a rubber block 215. The shift block 41 rotates in the large ring groove 43, and the shift block 41 drives the disc 44 to deflect the angle. The disc 44 drives the progressive thread strip 47 to deflect the angle synchronously. The progressive thread strip 47 squeezes the inner wall of the progressive thread groove 45 of the card plate 46, thereby causing the four card plates 46 to move linearly on the test tube rack 6, and the four card plates 46 drive The rubber block 2 15 limits the outside of the test tube, and the outer surface end position of the rubber block 2 15 does not exceed the outer end position of the rubber block 3 16. When a large-sized test tube needs to be fixed, the outer end face of the rubber block 2 15 is limited to the outside of the test tube by deflecting the dial block 41. When the card plate 46 moves, the movable block 49 is squeezed with the outer surface of the disc 44, so that the movable block 49 drives the telescopic column 39 to squeeze the spring 1 40, thereby adjusting the position of the movable block 49, and the movable block 49 drives the spring 2 48 to squeeze the rubber block 3 16, so that the bottom of the test tube is The upper end is squeezed and fixed, and the upper end is only limited. When the conical test tube is limited and fixed, the rubber block three 16 will move in the telescopic cavity two 50, and the rubber block two 15 and the rubber block three 16 are made of hard rubber on one end surface, and the surface contacting with the test tube is made of elastic rubber material, so that the test tube rack 6 can fix test tubes of different sizes and test tubes of different conical shapes, so that the test tube rack 6 can adjust the deflection angle of the dial block 41, so that the card plate 46 drives the rubber block two 15 to move, thereby adapting to the fixing requirements of test tubes of different sizes, whether it is a small-sized test tube or a large-sized test tube, can achieve stable limiting.

[0046] The inner wall of the movable cavity 1 28 and the inner wall of the movable cavity 2 29 are both installed with a stable limit assembly. The number of stable limit assemblies on the inner wall of the movable cavity 1 28 is several. The stable limit assembly includes a fixed column 37 and a telescopic cavity 1 38. The fixed column 37 is movably installed on the inner wall of the movable cavity 1 28. A telescopic cavity 1 38 is opened inside the fixed column 37. A spring 1 40 is installed on the inner wall of the telescopic cavity 1 38. The outer surface of the spring 1 40 is installed with a telescopic column 39. The outer surface of the several telescopic columns 39 inside the rocking platform 4 is commonly installed with a rubber block 1 42. The outer surface of the telescopic column 39 inside the card plate 46 is A movable block 49 is installed on the side surface, and the outer surface of the movable block 49 is in movable contact with the inner wall of the movable cavity 29. A telescopic cavity 2 50 is opened inside the movable block 49, and springs 2 48 are evenly installed on the inner wall of the telescopic cavity 2 50. The outer surfaces of several springs 2 48 are commonly installed with rubber blocks 3 16, and the outer surface of the rubber block 3 16 is in movable contact with the inner wall of the telescopic cavity 2 50. The test tube rack 6 is limited and fixed by the stable limit assembly, and the test tube rack 6 is protected from shaking by the action of the spring 1 40, and the spring 1 40 drives the telescopic column 39 and the rubber block 1 42 to quickly reset.

[0047] The working process of the present invention is as follows: when it is necessary to perform a resuscitation operation on cells revived in a test tube, the test tube is inserted into the test tube insertion hole 7 of the test tube rack 6, the bottom surface of the test tube is brought into active contact with the inner surface of the test tube rack 6, and the dial block 41 is manually moved. The dial block 41 rotates in the large annular groove 43, and the dial block 41 drives the disc 44 to deflect at an angle. The disc 44 drives the progressive thread strip 47 to deflect at an angle synchronously. The progressive thread strip 47 squeezes the inner wall of the progressive thread groove 45 of the card plate 46, so that the four card plates 46 move linearly on the test tube rack 6, and the four card plates 46 correspondingly drive the rubber block 15 The outer side of the test tube is limited, and the outer surface end surface position of the rubber block 2 15 does not exceed the outer end surface position of the rubber block 3 16. When a large-sized test tube needs to be fixed, the outer end surface of the rubber block 2 15 is limited to the outer side of the test tube by deflecting the dial block 41. When the card plate 46 moves, the movable block 49 is squeezed with the outer surface of the disc 44, so that the movable block 49 drives the telescopic column 39 to squeeze the spring 1 40, thereby adjusting the position of the movable block 49, and the movable block 49 drives the spring 2 48 to squeeze the rubber block 3 16, so that the bottom end of the test tube is squeezed and fixed, and the upper end is Only the position is limited, and when the conical test tube is fixed, the rubber block 3 16 will move in the telescopic cavity 2 50, and the rubber block 2 15 and the rubber block 3 16 are made of hard rubber on one end surface, and the surface in contact with the test tube is made of elastic rubber material, so that the test tube rack 6 can fix test tubes of different sizes and test tubes of different conical shapes, so that the test tube rack 6 can adjust the deflection angle of the dial block 41, so that the card plate 46 drives the rubber block 2 15 to move, thereby adapting to the fixing requirements of test tubes of different sizes, whether it is a small-sized test tube or a large-sized test tube, stable position limitation can be achieved, and the rubber block 3 16 can be fixed in the telescopic cavity 2 50 The second rubber block 15 and the third rubber block 16 are a composite structure in which one end surface is hard rubber and the surface in contact with the test tube is elastic rubber. The hard rubber part provides sufficient support and fixing force, and the elastic rubber part can better fit the surface of the test tube to avoid damage to the test tube and enhance the stability of the fixation. The upper end is limited and the bottom end is fixed, which not only ensures the stability of the test tube in the test tube rack 6, but also avoids unnecessary pressure on the test tube due to excessive fixation, which is beneficial to the cell recovery operation.

[0048] The experimenter controls the hydraulic press 2 through the controller to work, and the hydraulic press 2 drives the connecting block 1 3 and the shaking table 4 to move up and down, and the shaking table 4 drives the two connecting blocks 2 10 to move up and down synchronously, and the two connecting blocks 2 10 correspondingly drive the guide column 11 to move up and down, and the two guide columns 11 correspondingly drive the other two connecting blocks 2 10 to move up and down, and the four connecting blocks 2 10 correspondingly drive the limit slider 9 to move up and down stably on the limit slide rail 8, thereby making the main water bath pot cover 12 and the shaking table 4 move up and down, and the auxiliary water bath pot cover 14 is deflected at an angle through the hinge 13, so that the experimenter can grab the handle 26 of the test tube rack 6 and insert the test tube rack 6 into the slot 5 of the shaking table 4. The test tube rack 6 is provided with an inclined surface 27, which makes it convenient to insert the test tube rack 6 into the shaking table 4, and also makes it convenient to take the test tube rack 6 out of the shaking table 4, and then The hydraulic press 2 is controlled by the controller to work, so that the shaking table 4 drives the test tube rack 6 to move into the water bath shell 1. When the shaking table 4 drives the test tube rack 6 to move into the water bath, the main water bath cover 12 and the auxiliary water bath cover 14 are also moved to the top surface of the water bath shell 1 synchronously, thereby shielding the water bath shell 1. When the test tube cells burst during the recovery process, the main water bath cover 12 and the auxiliary water bath cover 14 provide shielding protection without causing personal injury to the operator, reducing damage to the experimental environment and other equipment. The hydraulic press 2 achieves precise speed and position control through the controller to ensure a smooth lifting process of the shaking table 4 and the main water bath cover 12 and the auxiliary water bath cover 14. The guiding function of the guide column 11 ensures straightness and stability during the lifting process, effectively preventing mechanical failures or safety accidents caused by deviation or shaking.

[0049] During the lifting and lowering of the rocking table 4, the rocking table 4 stably drives the sealing sleeve block 22 to lift and lower, and the inner surface of the sealing sleeve block 22 squeezes and lifts the bottom surface of the transmission shaft 21, so that the transmission shaft 21 drives the two clamping blocks 20 to lift and lower in the card slot 19 of the rotating shaft 18, and the motor 17 is controlled by the controller to work, and the motor 17 drives the rotating shaft 18 to rotate, and the rotating shaft 18 drives the card slot 19 to rotate, and the inner wall of the card slot 19 squeezes the outer sides of the two clamping blocks 20, so that the two clamping blocks 20 drive the transmission shaft 21 to rotate, and the transmission shaft 21 drives the two main bevel gears 23 to rotate, and the two main bevel gears 23 correspond to the outer sides of the slave bevel gears 24 The two gears are meshed with each other, and one main bevel gear 23 drives one slave bevel gear 24 to rotate, and the slave bevel gear 24 drives one transmission shaft 25 to rotate, and the transmission shaft 25 drives the column 31 to rotate, and the column 31 drives the outer reciprocating groove 32 to rotate, and the reciprocating groove 32 squeezes the reciprocating plate, so that the reciprocating clamping plate 33 drives the top column 34 to move back and forth linearly, and the top column 34 drives the clamping block 2 35 to move back and forth linearly in the clamping groove 2 36 in the sealing sleeve block 22, and the top column 34 performs high-speed reciprocating linear motion to squeeze the outside of the test tube rack 6, and the impact angle of the top column 34 forms an acute angle with the end face of the test tube rack 6, so that the test tube rack 6 shakes in the slot 5, and the test tube rack 6 shakes on all sides during the shaking process. The rubber block 142 is squeezed, so that the rubber block 142 squeezes the telescopic column 39, and the telescopic column 39 moves in the telescopic cavity 138, and the rubber block 142 moves in the movable cavity 128, and the test tube rack 6 is limited and fixed by the stable limit assembly, and the test tube rack 6 is protected from shaking by the action of the spring 140, and the spring 140 drives the telescopic column 39 and the rubber block 142 to quickly reset, and the impact angle of the top column 34 forms an acute angle with the end face of the test tube rack 6, so that the direction of the force applied to the test tube rack 6 is changed to left and right and front and back shaking, and the top column 34 hits the end face of the test tube rack 6 at an acute angle, decomposing the single impact force into horizontal and vertical components, driving the test tube rack 6 to swing in the left and right directions and driving the test tube rack 6 to slightly move in the front and back directions. Vibration is caused to superimpose the motions of the test tube rack 6, and a composite motion trajectory of "left-right swing + front-back axis oscillation" is achieved through uniaxial impact, simulating artificial three-dimensional shaking motion, forming a spiral rising vortex, covering the bottom, edge and liquid surface area of ​​the test tube, avoiding mixing dead corners caused by static or uniaxial oscillation, and the acceleration direction of the composite motion continuously changes, avoiding the periodic unidirectional stress concentration of uniaxial oscillation, protecting fragile cells, and simulating the "shake-pause" rhythm of the artificial wrist. Pausing only requires controlling the motor 17 to stop working, and a plurality of springs 40 quickly stabilize the test tube rack 6, reducing the mechanical damage to the cell membrane caused by the rapid melting of ice crystals. The three-dimensional motion reduces the thickness of the thermal boundary layer near the test tube wall, shortens the thawing time, and improves the cell survival rate and functional integrity.

[0050] And another main helical gear 23 drives another from the bevel gear 24 rotation, from the bevel gear 24 drive another transmission shaft two 25 rotation, transmission shaft two 25 drive turbine 30 rotation, turbine 30 to the laboratory water bath inside the water is stirred, so that the test tube outside the water temperature heat exchange, test tube three-dimensional oscillation of the complex motion of the cell suspension to promote uniform mixing, test tube outside the turbine 30 stirring to ensure that the water bath temperature uniform, form "mixing outside uniform" synergistic effect, thereby shortening the cell recovery time, and the turbine 30 rotation forms a circular flow field, eliminate the traditional water bath cold and hot circulation dead zone.

[0051] It should be noted that the relational terms herein, such as first and second, are used solely to distinguish one from another entity or action without necessarily requiring or implying any actual relationship or order between such entities or actions. Moreover, the terms "comprises", "comprising", or any other variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but can include other elements not expressly listed or inherent to such process, method, article, or apparatus.

[0052] The preferred embodiments of the application disclosed above are only used to help explain the application. The preferred embodiments do not describe all the details of the application, nor limit the application to the specific embodiments described. Obviously, many modifications and variations can be made to the application according to the content of the specification. The specification selects and specifically describes these embodiments in order to better explain the principles and practical application of the application, so that those skilled in the art can well understand and utilize the application. The application is limited only by the claims and their full scope and equivalents.

Claims

1. A laboratory water bath, comprising a water bath housing (1), characterized in that: A hydraulic press (2) is mounted on the outer surface of the water bath shell (1), a connecting block (3) is mounted on one end surface of the output end of the hydraulic press (2), a shaking table (4) is mounted on the outer surface of the connecting block (3), a slot (5) is provided inside the shaking table (4), a test tube rack (6) is movably mounted on the inner wall of the slot (5), and multiple-specification fixing components are evenly mounted inside the test tube rack (6), and the multiple-specification fixing components facilitate the fixing of test tubes of different sizes for positional fixation; The inner surface of the water bath shell (1) is installed with a stabilizing lifting assembly, the outer surface of the stabilizing lifting assembly is installed with a main water bath cover (12), the outer surface of the water bath shell (1) is installed with a vibration power assembly, the outer surface of the vibration power assembly is installed with a shaking assembly, the shaking assembly includes a sealing sleeve (22) and two transmission shafts (25), the sealing sleeve (22) is installed on the outer surface of the shaking table (4), the two transmission shafts (25) are movably installed inside the sealing sleeve (22), one end surface of the transmission shaft (25) is installed with a turbine (30), the outer surface of the other transmission shaft (25) is installed with a turbine (30), and the outer surface of the other transmission shaft (25) is installed with a turbine (30). A column (31), wherein a reciprocating groove (32) is provided on the outer surface of the column (31), a reciprocating clamping plate (33) is movably mounted on the inner wall of the reciprocating groove (32), a top column (34) is mounted on one end surface of the reciprocating clamping plate (33), a second clamping block (35) is mounted on the outer surface of the top column (34), a second clamping groove (36) is provided inside the sealing sleeve block (22), the outer surface of the second clamping block (35) and the outer surface of the top column (34) are both in movably contact with the inner wall of the second clamping groove (36), one end surface of the top column (34) is in movably contact with the outer surface of the test tube rack (6), and the top column (34) forms an acute angle with the end surface of the test tube rack (6); The shaking assembly causes the column (31) to perform high-speed reciprocating motion to squeeze and vibrate the test tube rack (6), causing the test tube rack (6) to shake regularly. The turbine (30) drives the water flow to stir and perform heat exchange. The interior of the shaking table (4) is evenly distributed with an active cavity (28).

2. A laboratory water bath according to claim 1, characterized in that: The vibration power assembly includes a motor (17) and a rotating shaft (18), wherein the motor (17) is mounted on the outer surface of the water bath shell (1), and the rotating shaft (18) is mounted on the outer surface of the output end of the motor (17). A card slot (19) is provided inside the rotating shaft (18), and a transmission shaft (21) is movably mounted inside the sealing sleeve (22). Two card blocks (20) are mounted on the outer surface of the transmission shaft (21), and the outer surfaces of the two card blocks (20) and the outer surface of the transmission shaft (21) are both in movably contact with the inner wall of the card slot (19).

3. A laboratory water bath according to claim 2, characterized in that: Two main helical gears (23) are mounted on the outer surface of the transmission shaft 1 (21), and two slave helical gears (24) are mounted on the outer surfaces of the two transmission shafts 2 (25), and the outer surfaces of the two main helical gears (23) mesh with the outer surfaces of the two slave helical gears (24).

4. A laboratory water bath according to claim 1, characterized in that: The stable lifting assembly includes two limiting slide rails (8) and two guide columns (11), the two limiting slide rails (8) are installed on the inner surface of the water bath shell (1), the two guide columns (11) are movably installed inside the water bath shell (1), and the outer surfaces of the two guide columns (11) are both installed with two connecting blocks (10), wherein the outer surfaces of two connecting blocks (10) are fixedly connected to the outer surface of the shaking table (4), and the outer surfaces of the other two connecting blocks (10) are fixedly connected to the outer surface of the main water bath cover (12), and the outer surfaces of the four connecting blocks (10) are all installed with limiting sliders (9), and the outer surfaces of the limiting sliders (9) are in movably contact with the outer surfaces of the limiting slide rails (8).

5. A laboratory water bath according to claim 4, characterized in that: The outer surface of the main water bath lid (12) is evenly mounted with hinges (13), and the outer surfaces of several hinges (13) are commonly mounted with auxiliary water bath lids (14), and the bottom end surfaces of the auxiliary water bath lids (14) and the bottom end surface of the main water bath lid (12) are both in active contact with the top end surface of the water bath shell (1).

6. A laboratory water bath according to claim 1, characterized in that: Two handles (26) are installed on the top surface of the test tube rack (6), an inclined surface (27) is provided on the test tube rack (6), and test tube jacks (7) are evenly opened inside the test tube rack (6). The multi-specification fixing assembly includes a large annular groove (43) and a disc (44), the large annular groove (43) is opened on the top surface of the test tube rack (6), the disc (44) is movably installed inside the test tube rack (6), and a shift block (41) is installed on the top surface of the disc (44), and the outer surface of the shift block (41) is in movably contact with the inner wall of the large annular groove (43).

7. A laboratory water bath according to claim 6, characterized in that: A progressive thread strip (47) is movably mounted on the top surface of the disc (44), and four card blocks (46) are movably mounted inside the test tube rack (6). The bottom surfaces of the four card blocks (46) are all provided with progressive thread grooves (45), and the outer surfaces of the progressive thread strip (47) are in movably contact with the inner wall of the progressive thread groove (45). The interiors of the four card blocks (46) are all provided with movable cavities (29), and one end surface of the four card blocks (46) is all provided with rubber blocks (15).

8. A laboratory water bath according to claim 7, characterized in that: The inner wall of the movable cavity 1 (28) and the inner wall of the movable cavity 2 (29) are both installed with stable limiting components, and the number of stable limiting components on the inner wall of the movable cavity 1 (28) is several.

9. A laboratory water bath according to claim 8, characterized in that: The stabilizing limit assembly includes a fixed column (37) and a telescopic cavity (38), wherein the fixed column (37) is movably mounted on the inner wall of the movable cavity (28), a telescopic cavity (38) is provided inside the fixed column (37), a spring (40) is mounted on the inner wall of the telescopic cavity (38), a telescopic column (39) is mounted on the outer surface of the spring (40), a rubber block (42) is mounted on the outer surface of several telescopic columns (39) inside the shaking table (4), a movable block (49) is mounted on the outer surface of the telescopic column (39) inside the card plate (46), and the outer surface of the movable block (49) is in movably contact with the inner wall of the movable cavity (29).

10. A laboratory water bath according to claim 9, characterized in that: A telescopic cavity 2 (50) is provided inside the movable block (49), and springs 2 (48) are evenly installed on the inner wall of the telescopic cavity 2 (50). Rubber blocks 3 (16) are commonly installed on the outer surfaces of a plurality of springs 2 (48), and the outer surfaces of the rubber blocks 3 (16) are in movable contact with the inner wall of the telescopic cavity 2 (50).

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