Preparation method of anti-aging rhizoma polygonati extract, product containing anti-aging rhizoma polygonati extract and application of anti-aging rhizoma polygonati extract
By combining microbial fermentation and enzymatic hydrolysis with clarification and decolorization steps, a high-polysaccharide, high-protein, and water-soluble Polygonatum extract was prepared, solving the problem of low polysaccharide extraction rate in existing Polygonatum processing and realizing the industrial application of Polygonatum.
Patent Information
- Application Number
- CN202511191444.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-08-25
- Publication Date
- 2025-10-17
AI Technical Summary
The existing processing technology of Polygonatum sibiricum has low polysaccharide extraction rate, low protein content and poor water solubility, which is not conducive to industrial promotion.
Microbial fermentation technology was used to prepare Polygonatum extract through the combined fermentation of Aspergillus edodes, Weizmannii coagulans and Lactobacillus gasseri, combined with enzymatic hydrolysis by xylanase, cellulase and glucanase. Subsequently, the extract was clarified with guar gum, senna gum and cassia gum, decolorized with activated clay, and finally spray-dried.
It improves the polysaccharide extraction rate and protein content of Polygonatum extract, enhances water solubility, and is suitable for industrial production and food applications.
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Figure CN120789175A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of microbial fermentation, in particular to a preparation method of an anti-aging Rhizoma Polygonati extract, a product containing the same and application. BACKGROUND
[0002] China is one of the countries with high degree of population aging in the world, with the largest number of elderly population, the fastest aging speed and the heaviest task of coping with population aging. Therefore, anti-aging has become a hot spot pursued by the market. Anti-aging refers to taking various measures to slow down the process of physical aging and reduce the negative effects of aging-related changes on body function and appearance, so as to maintain good health and quality of life.
[0003] Rhizoma Polygonati has been recorded as a tonic medicine in the herbal works since the end of the Eastern Han Dynasty, and it is also often used as "the essence of grass and tea" for consumption. Studies have shown that long-term consumption of Rhizoma Polygonati can delay aging. However, the current processing technology of Rhizoma Polygonati is mainly primary processing. The products obtained by this processing technology (such as nine steaming and nine drying Rhizoma Polygonati, Rhizoma Polygonati pills, Rhizoma Polygonati wine, etc.) have low polysaccharide extraction rate, low protein content, low yield and poor water solubility, which is not conducive to industrial promotion. SUMMARY
[0004] To at least partially solve the above problems, according to the first aspect of the present application, the present application provides a preparation method of an anti-aging Rhizoma Polygonati extract, comprising:
[0005] Step S1, obtaining Rhizoma Polygonati after crushing and grinding;
[0006] Step S2, inoculating Aspergillus shirousami with an initial concentration of 1-8×10 6 cfu / g in the Rhizoma Polygonati after crushing and grinding, inoculating amount of 1-5% w / v, adjusting pH value to 4-6, temperature of 30-60℃, and fermenting for 2-6 days to obtain a primary fermentation liquor;
[0007] Step S3, adjusting the pH value of the primary fermentation liquor to 4.5-6.5, temperature to 40-60℃, adding 0.5-10% w / v of xylanase, cellulase and glucanase, the adding ratio of xylanase, cellulase and glucanase being 1:1-5:1-5, and enzymolysis time being 2-6h to obtain an enzymolysis liquor;
[0008] Step S4, inoculating Weissella coagulans with an initial concentration of 1-8×10 9 cfu / g and Lactobacillus gasseri with an initial concentration of 1-8×10 9 cfu / g in the enzymolysis liquor, inoculating amount of 0.5-5% w / v, the ratio of Weissella coagulans and Lactobacillus gasseri being 1:1-4, adjusting pH value to 2-5, temperature to 25-45℃, and fermenting for 2-6 days to obtain a secondary fermentation liquor;
[0009] Step S5, clarifying the secondary fermentation liquid to obtain a clarified liquid;
[0010] Step S6, decolorizing the clarified liquid to obtain a decolorized liquid;
[0011] Step S7: purifying the decolorized liquid to obtain the polygonatum extract.
[0012] Preferably, in step S2, the initial concentration of the inoculated solution in the ground polygonatum is 1-8×10 6 aegypti cfu / g, the inoculation amount is 3-5% w / v, the pH value is adjusted to 4.5-5.5, the temperature is 45-55°C, and the fermentation is carried out for 3-5 days to obtain a primary fermentation liquid.
[0013] Preferably, in step S3, the pH value of the primary fermentation broth is adjusted to 4.5-5.0, the temperature is 45-50°C, 1-3% w / v of xylanase, cellulase, and glucanase are added, the addition ratio of xylanase, cellulase, and glucanase is 1:2-4:1-3, and the enzymatic hydrolysis time is 4-6 hours to obtain an enzymatic hydrolyzate.
[0014] Preferably, in step S4, the initial inoculation concentration in the enzymatic solution is 1-8×10 9 cfu / g of Weizmannella coagulans and an initial concentration of 1-8 × 10 9 cfu / g of Lactobacillus gasseri, the inoculation amount is 1-3% w / v, the ratio of Weizmannella coagulans to Lactobacillus gasseri is 1:2-3, the pH value is adjusted to 3-4, the temperature is 35-40°C, and the fermentation is carried out for 3-5 days to obtain a secondary fermentation liquid.
[0015] Preferably, in step S1, obtaining the crushed and ground Polygonatum sibiricum comprises:
[0016] Use fresh or dried polygonatum as raw material, wash with 2-20 times water to remove foreign matter;
[0017] The cleaned raw materials are added into a grinder and crushed into 30-300 mesh particles, and then 2-20 times of water is added to pass through a colloid mill and ground into 2-50 micron particles, and then heated to 85-100° C. and kept warm for 15-30 minutes for sterilization to obtain the crushed and ground Polygonatum sibiricum.
[0018] Preferably, in step S5, clarifying the secondary fermentation broth to obtain a clarified liquid comprises:
[0019] The 0.5-3% of gellan gum, inulina and gellan gum are added into the secondary fermentation liquor, the ratio of gellan gum, inulina and gellan gum is 1:1-4:1-5, and the supernatant is taken as the clarified liquid after standing at room temperature for 2-8 hours. Preferably, the 1-3% of gellan gum, inulina and gellan gum are added into the secondary fermentation liquor, the ratio of gellan gum, inulina and gellan gum is 1:2-3:2-4, and the supernatant is taken as the clarified liquid after standing at room temperature for 3-6 hours.
[0020] Preferably, the step S6 of decolorizing the clarified liquid to obtain the decolorized liquid comprises:
[0021] The 0.5-5% of activated white clay is added into the clarified liquid, the temperature is controlled at 55-65℃, and the decolorization is performed for 30-90 minutes, and then the plate and frame filtration is performed to obtain the decolorized liquid. Preferably, the 1-3% of activated white clay is added into the clarified liquid, the temperature is controlled at 55-65℃, and the decolorization is performed for 40-60 minutes, and then the plate and frame filtration is performed to obtain the decolorized liquid.
[0022] Preferably, the step S7 of refining the decolorized liquid to obtain the polygonatum extract comprises:
[0023] The decolorized liquid is concentrated and then spray dried to obtain the polygonatum extract.
[0024] According to the second aspect of the present application, the present application provides a product containing the polygonatum extract prepared by the above preparation method. Preferably, the product can be a tablet, a powder, an oral liquid or a general food.
[0025] According to the third aspect of the present application, the present application provides the use of the above product in anti-aging.
[0026] The preparation method of the anti-aging polygonatum extract provided by the present application has high polysaccharide extraction rate, high protein content, high yield, and good water solubility, which is beneficial to industrial promotion. The polygonatum extract prepared by the preparation method provided by the present application contains rich polysaccharides and polypeptides, is completely soluble in water, is resistant to high temperature, has small molecular weight, is easy to absorb, can be processed into tablets, powders and oral liquids, and can also be used in general foods to meet the needs of people in anti-aging. BRIEF DESCRIPTION OF DRAWINGS
[0027] Figure 1 is the survival number of nematodes versus days curve provided by the present application. DETAILED DESCRIPTION
[0028] The technical solutions of the preferred embodiments of the present application will be clearly and completely described below with reference to the drawings. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments of the present application, all other embodiments obtained by a person of ordinary skill in the art without creative work fall within the scope of the present application. In the following examples and comparative examples, if no special description is given, the used operation methods are all conventional operation methods, the used equipment is all conventional equipment, the used materials are all common commercially available products, and the used equipment materials are all the same.
[0029] In the following examples and comparative examples, Aspergillus sydowii was purchased from the Plasmid Collection, Aspergillus sp. strain resources company, enzyme preparations were purchased from Ningxia Xiasen Industrial Group Co., Ltd., Weizmannia coagulans was purchased from Sichuan Huanxi Biological Technology Co., Ltd., Lactobacillus gasseri was purchased from Minsheng Zhongke Jiayi (Shandong) Biological Engineering Co., Ltd., gellan gum and xanthan gum were purchased from Zhengzhou Jiuting Chemical Product Co., Ltd., and locust bean gum was purchased from Shandong Pingju Biological Technology Co., Ltd.
[0030] Example 1
[0031] Raw material cleaning: 100 g of fresh polygonatum sibiricum was weighed, and cleaned with 5 times water for 2 times to remove dirt and other impurities.
[0032] Crushing and sterilization: the cleaned polygonatum sibiricum was crushed into 30-300 mesh particles in a crusher, and then passed through a colloid mill with 10 times water to grind into 2-50 micron particles, and then sterilized at 90℃ for 20 min;
[0033] Preparation of primary fermentation broth: Aspergillus sydowii was inoculated into the sterilized polygonatum sibiricum solid-liquid mixture, the inoculation amount was 3%, 5×10 6 cfu / g, the pH value was adjusted to 4.5, the temperature was 45℃, and the fermentation was carried out for 3 days to obtain the primary fermentation broth;
[0034] Preparation of enzyme hydrolysate: the pH value of the primary fermentation broth was adjusted to 4.5, the temperature was 50℃, 1% (w / v) of xylanase, cellulase and glucanase were added, the addition ratio was 1:2:1, and the enzyme hydrolysis time was 4 h to obtain the enzyme hydrolysate;
[0035] Preparation of secondary fermentation broth: Weizmannia coagulans (5×10 9 cfu / g) and Lactobacillus gasseri (5×10 9 cfu / g) were inoculated into the enzyme hydrolysate, the inoculation amount was 1% w / v, the ratio was 1:2, the pH value was adjusted to 3, the temperature was 35℃, and the fermentation was carried out for 3 days to obtain the secondary fermentation broth;
[0036] Clarification of the secondary fermentation broth: 1% of gellan gum, xanthan gum and locust bean gum (ratio 1:2:2) were added to the secondary fermentation broth, and the mixture was left to stand at room temperature for 3 h, and the supernatant was taken as the clarified broth;
[0037] Decolorization of the clarified liquid: 1% activated clay was added to the clarified liquid, temperature 60°C, decolorization 40 min, plate and frame filtration, to obtain the decolorized liquid;
[0038] Spray drying of the decolorized liquid: the decolorized liquid was concentrated and then spray dried to obtain the extract of Rhizoma Polygonati.
[0039] Example 2
[0040] Raw material washing: 100 g of fresh Rhizoma Polygonati was weighed and washed twice with 5 times water to remove dirt and other impurities.
[0041] Crushing and sterilization: the washed Rhizoma Polygonati was crushed into 30-300 mesh particles, and then added to a colloidal mill with 10 times water to grind into 2-50 micron particles, and then sterilized at 90°C for 20 min;
[0042] Preparation of the primary fermentation liquid: the sterilized Rhizoma Polygonati solid-liquid mixture was inoculated with Aspergillus shirousami, with an inoculation amount of 5%, 5 x 10 6 cfu / g, the pH value was adjusted to 5.5, the temperature was 55°C, and the fermentation was carried out for 5 days to obtain the primary fermentation liquid;
[0043] Preparation of the enzymatic hydrolysis liquid: the pH value of the primary fermentation liquid was adjusted to 5.0, the temperature was 50°C, 3% (w / v) of xylanase, cellulase and glucanase were added in a ratio of 1:4:3, and the enzymatic hydrolysis was carried out for 6 h to obtain the enzymatic hydrolysis liquid;
[0044] Preparation of the secondary fermentation liquid: Aspergillus coagulans (5 x 10 9 cfu / g) and Lactobacillus gasseri (5 x 10 9 cfu / g) were inoculated in the enzymatic hydrolysis liquid, with an inoculation amount of 3% w / v and a ratio of 1:3, the pH value was adjusted to 4, the temperature was 40°C, and the fermentation was carried out for 5 days to obtain the secondary fermentation liquid;
[0045] Clarification of the secondary fermentation liquid: 3% of curdlan, ghatti gum and tragacanth gum (in a ratio of 1:3:4) were added to the secondary fermentation liquid, and the mixture was left to stand at room temperature for 6 h, and the supernatant was taken as the clarified liquid;
[0046] Decolorization of the clarified liquid: 3% activated clay was added to the clarified liquid, temperature 60°C, decolorization 60 min, plate and frame filtration, to obtain the decolorized liquid;
[0047] Spray drying of the decolorized liquid: the decolorized liquid was concentrated and then spray dried to obtain the extract of Rhizoma Polygonati.
[0048] Comparative Example 1
[0049] Raw material washing: 100 g of fresh Rhizoma Polygonati was weighed and washed twice with 5 times water to remove dirt and other impurities.
[0050] Sterilization by pulverization: the washed Polygonatum sibiricum was pulverized into 30-300 mesh particles, and then added to a colloidal mill with 10 times water to grind into 2-50 micron particles, and then sterilized at 90℃ for 20 min;
[0051] Preparation of enzyme hydrolysate: the pH value of the pulverized and ground Polygonatum sibiricum was adjusted to 4.5, the temperature was 50℃, 1% (w / v) of xylanase, cellulase and glucanase were added at a ratio of 1:2:1, and the enzyme hydrolysis time was 4 h to obtain the enzyme hydrolysate;
[0052] Preparation of fermentation broth: the enzyme hydrolysate was inoculated with W. coagulans (5×10 9 cfu / g) and L. gasseri (5×10 9 cfu / g) at an inoculation amount of 1% w / v and a ratio of 1:2, the pH value was adjusted to 3, the temperature was 35℃, and the fermentation was carried out for 3 days to obtain the fermentation broth;
[0053] Clarification of secondary fermentation broth: 1% of curdlan, ghatti gum and tragacanth gum (at a ratio of 1:2:2) was added to the fermentation broth, and the mixture was left to stand at room temperature for 3 h, and the supernatant was taken as the clarified broth;
[0054] Decolorization of the clarified broth: 1% of activated white clay was added to the clarified broth, the temperature was 60℃, and the decolorization was carried out for 40 min, and then the mixture was filtered by plate and frame to obtain the decolorized broth;
[0055] Spray drying of the decolorized broth: the decolorized broth was concentrated and then spray dried to obtain the Polygonatum sibiricum extract.
[0056] Comparative Example 2
[0057] Cleaning of raw materials: 100 g of fresh Polygonatum sibiricum was cleaned with 5 times water for 2 times to remove dirt and other impurities.
[0058] Sterilization by pulverization: the washed Polygonatum sibiricum was pulverized into 30-300 mesh particles, and then added to a colloidal mill with 10 times water to grind into 2-50 micron particles, and then sterilized at 90℃ for 20 min;
[0059] Preparation of primary fermentation broth: Aspergillus shirousami was inoculated into the sterilized Polygonatum sibiricum solid-liquid mixture at an inoculation amount of 3% and 5×10 6 cfu / g, the pH value was adjusted to 4.5, the temperature was 45℃, and the fermentation was carried out for 3 days to obtain the primary fermentation broth;
[0060] Preparation of secondary fermentation broth: W. coagulans (5×10 9 cfu / g) and L. gasseri (5×10 9 cfu / g) were inoculated into the primary fermentation broth at an inoculation amount of 1% w / v and a ratio of 1:2, the pH value was adjusted to 3, the temperature was 35℃, and the fermentation was carried out for 3 days to obtain the secondary fermentation broth;
[0061] Clarification of the secondary fermentation broth: 1% of the available gelatin, alstonia scholaris gum and cassia gum (ratio 1:2:2) was added to the secondary fermentation broth, and it was left at room temperature for 3 hours. The supernatant was taken as the clarified liquid;
[0062] Decolorization of the clarified liquid: 1% of activated white clay was added to the clarified liquid, and it was decolorized at 60°C for 40 minutes. Plate and frame filtration was performed to obtain the decolorized liquid;
[0063] Spray drying of the decolorized liquid: the decolorized liquid was concentrated and then spray dried to obtain the rhizoma polygonati extract.
[0064] Comparative Example 3
[0065] Raw material cleaning: 100 g of fresh rhizoma polygonati was cleaned twice with 5 times the amount of water to remove dirt and other impurities.
[0066] Crushing and sterilization: the cleaned rhizoma polygonati was crushed into 30-300 mesh particles, and then passed through a colloid mill with 10 times the amount of water to grind into 2-50 micron particles. The particles were then sterilized by heating to 90°C for 20 minutes.
[0067] Preparation of the primary fermentation broth: the sterilized rhizoma polygonati solid-liquid mixture was inoculated with Aspergillus shirousami, and the inoculation amount was 3%, 5×10 6 cfu / g. The pH value was adjusted to 4.5, and the temperature was 45°C. Fermentation was performed for 3 days to obtain the primary fermentation broth.
[0068] Preparation of the enzymatic hydrolysis liquid: the pH value of the primary fermentation broth was adjusted to 4.5, and the temperature was 50°C. 1% (w / v) of xylanase, cellulase and glucanase was added in a ratio of 1:2:1, and the enzymatic hydrolysis time was 4 hours to obtain the enzymatic hydrolysis liquid.
[0069] Clarification of the enzymatic hydrolysis liquid: 1% of the available gelatin, alstonia scholaris gum and cassia gum (ratio 1:2:2) was added to the enzymatic hydrolysis liquid, and it was left at room temperature for 3 hours. The supernatant was taken as the clarified liquid.
[0070] Decolorization of the clarified liquid: 1% of activated white clay was added to the clarified liquid, and it was decolorized at 60°C for 40 minutes. Plate and frame filtration was performed to obtain the decolorized liquid.
[0071] Spray drying of the decolorized liquid: the decolorized liquid was concentrated and then spray dried to obtain the rhizoma polygonati extract.
[0072] Comparative Example 4
[0073] Raw material cleaning: 100 g of fresh rhizoma polygonati was cleaned twice with 5 times the amount of water to remove dirt and other impurities.
[0074] Crushing and sterilization: the cleaned rhizoma polygonati was crushed into 30-300 mesh particles, and then passed through a colloid mill with 10 times the amount of water to grind into 2-50 micron particles. The particles were then sterilized by heating to 90°C for 20 minutes.
[0075] Preparation of primary fermentation broth: inoculate Aspergillus shirousami with the sterilized mixture of Polygonatum sibiricum and liquid medium at an inoculation amount of 3%, 5×10 6 cfu / g, adjust pH value to 4.5, temperature to 45℃, and ferment for 3 days to obtain the primary fermentation broth;
[0076] Preparation of enzymatic hydrolysis broth: adjust pH value of the primary fermentation broth to 4.5, temperature to 50℃, and add 1% (w / v) of xylanase, cellulase, and glucanase at a ratio of 1:2:1, and enzymatic hydrolysis for 4h to obtain the enzymatic hydrolysis broth;
[0077] Preparation of secondary fermentation broth: inoculate the enzymatic hydrolysis broth with Weissella coagulans (5×10 9 cfu / g) and Lactobacillus gasseri (5×10 9 cfu / g) at an inoculation amount of 1% w / v and a ratio of 1:2, adjust pH value to 3, temperature to 35℃, and ferment for 3 days to obtain the secondary fermentation broth;
[0078] Decolorization of the secondary fermentation broth: add 1% of activated white clay to the secondary fermentation broth, temperature to 60℃, and decolorize for 40min, and then perform plate and frame filtration to obtain the decolorized broth;
[0079] Spray drying of the decolorized broth: concentrate the decolorized broth and then perform spray drying to obtain the Polygonatum sibiricum extract.
[0080] Experiment 1: detection of the Polygonatum sibiricum extract
[0081] The Polygonatum sibiricum extract samples obtained from Example 1, Example 2, Comparative Example 1, Comparative Example 2, Comparative Example 3, and Comparative Example 4 were detected, and the detection contents included sensory index, state of 10% solution, polysaccharide extraction rate, protein content, and yield. The polysaccharide extraction rate was determined according to the method of phenol-sulfuric acid-ultraviolet spectrophotometer, and the protein content was determined according to GB 5009.5. The detection results are shown in Table 1.
[0082] Table 1
[0083] Group Sensory index 10% solution Polysaccharide extraction rate (%) Protein content (%) Yield (%) Example 1 White powder, no odor, slightly sweet taste Solution transparent at room temperature, transparent after heating at 90°C for 20 min 40.8% 5.3% 59.7% Example 2 White powder, no odor, slightly sweet taste Solution transparent at room temperature, transparent after heating at 90°C for 20 min 45.2% 5.6% 63.5% Comparative Example 1 White powder, no odor, tingling taste Solution transparent at room temperature, transparent after heating at 90°C for 20 min 28.7% 4.6% 40.6% Comparative Example 2 Off-white powder, odor, no sweet taste, tingling Solution transparent at room temperature, transparent after heating at 90°C for 20 min 10.4% 4.1% 16.8% Comparative Example 3 Off-white powder, odor, no sweet taste, tingling Solution transparent at room temperature, transparent after heating at 90°C for 20 min 19.3% 4.3% 32.2% Comparative Example 4 Off-white powder, odor, bitter taste Solution not transparent at room temperature, flocculation after heating at 90°C for 20 min 38.6% 4.9% 61.4%
[0084] As can be seen from Table 1, the yields of Example 1 and Example 2 are both above 50%, the polysaccharide extraction rate of the main effective component of Polygonatum sibiricum is above 40%, the protein content is high, the solution is transparent, the taste is good, and it is resistant to high temperature, which is suitable for industrial production and application in food. The yields of Comparative Examples 1-3 are lower than 50%, the polysaccharide content is lower than 30%, and the protein content is low, which is not suitable for industrial production. The yield of Comparative Example 4 is relatively high, but the solution is not transparent, flocculation occurs upon heating, and the taste is bitter, which is also not suitable for industrial promotion.
[0085] Experiment 2: study on anti-aging effect of the Polygonatum sibiricum extract
[0086] The synchronized C. elegans (purchased from Fujian Shangyuan Bioscience Technology Co., Ltd.) were randomly divided into 4 groups, 100 worms in each group, which were blank group and Example 1 group, Example 2 group, Comparative Example 1 group respectively. The NGM medium of the blank group did not add the extract of Polygonatum, and the NGM medium of Example 1 group, Example 2 group and Comparative Example 1 group added the extract of Polygonatum prepared by Example 1, Example 2 and Comparative Example 1 respectively (the addition amount was 100 mg / mL). Then the worms in each group were transferred to the corresponding NGM medium, and then cultured in a 20℃ artificial climate box, which was recorded as day 0. The NGM medium was replaced every 2d (the replaced NGM medium of each group was the same as the initial NGM medium of each group) and the number of dead and live worms was recorded (the platinum wire was gently touched the worms without reaction, which was considered as dead worms), until all the worms died. According to the survival number of the worms and the corresponding days, the survival curve was drawn, as shown in FIG. 1, and the average lifespan and the maximum lifespan of the worms in each group were obtained by analyzing the curve and data. The experimental results are shown in Table 2. Figure 1
[0087] Table 2
[0088] Group Average life (days) Longest life (days) Blank group 11.06±0.25 13.43±0.63 Example 1 group <![CDATA[21.53±0.85 #* ]]> 24.25 ± 0.74 #* ]] Example 2 group 22.81 ± 0.97 #* ]] 25.33 ± 0.64 #* ]] Comparative Example 1 group 14.48±1.27 17.24±1.42
[0089] Note: # indicates P<0.01 compared with the blank group; * indicates P<0.05 compared with Comparative Example 1 group.
[0090] From Table 2, it can be seen that the lifespan of the worms in Example 1 group and Example 2 group was significantly higher than that of the blank group (P<0.01) and Comparative Example 1 group (P<0.05), and the lifespan of the worms in Comparative Example 1 group had a trend of prolonging compared with the blank group, but there was no significant difference statistically. This shows that the extract of Polygonatum prepared by Example 1 and Example 2 can significantly prolong the lifespan of the worms and has an anti-aging effect.
[0091] Based on the above embodiments of the present application, one technical feature of one embodiment can be beneficially combined with one or more other embodiments without explicitly denying or conflicting.
[0092] Although some specific embodiments of the present application have been described in detail by examples, those skilled in the art should understand that the above examples are only for illustration, not for limiting the scope of the present application. Those skilled in the art should understand that the above embodiments can be modified or some technical features can be replaced equivalently without departing from the scope and spirit of the present application. The scope of the present application is defined by the appended claims.
Claims
1. A method for preparing an anti-aging Polygonatum sibiricum extract, characterized in that: include: Step S1, obtaining the crushed and ground polygonatum; Step S2: inoculate the ground polygonatum with an initial concentration of 1-8×10 6 cfu / g of Aspergillus aegypti, the inoculation amount is 1-5% w / v, the pH value is adjusted to 4-6, the temperature is 30-60°C, and the fermentation is carried out for 2-6 days to obtain a primary fermentation liquid; Step S3, adjusting the pH value of the primary fermentation broth to 4.5-6.5 and the temperature to 40-60° C., adding 0.5-10% w / v of xylanase, cellulase, and glucanase, with the addition ratio of xylanase, cellulase, and glucanase being 1:1-5:1-5, and performing enzymolysis for 2-6 hours to obtain an enzymolysis solution; Step S4: the initial inoculation concentration in the enzymatic solution is 1-8×10 9 cfu / g of Weizmannella coagulans and an initial concentration of 1-8 × 10 9 cfu / g of Lactobacillus gasseri, an inoculation amount of 0.5-5% w / v, a ratio of Weizmannella coagulans to Lactobacillus gasseri of 1:1-4, adjusting the pH to 2-5, the temperature to 25-45°C, and fermenting for 2-6 days to obtain a secondary fermentation liquid; Step S5, clarifying the secondary fermentation liquid to obtain a clarified liquid; Step S6, decolorizing the clarified liquid to obtain a decolorized liquid; Step S7: purifying the decolorized liquid to obtain the polygonatum extract.
2. The preparation method according to claim 1, characterized in that In step S2, the initial concentration of inoculation is 1-8×10 6 aegypti cfu / g, the inoculation amount is 3-5% w / v, the pH value is adjusted to 4.5-5.5, the temperature is 45-55°C, and the fermentation is carried out for 3-5 days to obtain a primary fermentation liquid.
3. The preparation method according to claim 1, characterized in that In step S3, the pH value of the primary fermentation broth is adjusted to 4.5-5.0, the temperature is 45-50° C., 1-3% w / v of xylanase, cellulase, and glucanase are added, the addition ratio of xylanase, cellulase, and glucanase is 1:2-4:1-3, and the enzymatic hydrolysis time is 4-6 hours to obtain an enzymatic hydrolyzate.
4. The preparation method according to claim 1, characterized in that In step S4, the initial inoculation concentration in the enzymatic solution is 1-8×10 9 cfu / g of Weizmannella coagulans and an initial concentration of 1-8 × 10 9 cfu / g of Lactobacillus gasseri, the inoculation amount is 1-3% w / v, the ratio of Weizmannella coagulans to Lactobacillus gasseri is 1:2-3, the pH value is adjusted to 3-4, the temperature is 35-40°C, and the fermentation is carried out for 3-5 days to obtain a secondary fermentation liquid.
5. The preparation method according to claim 1, characterized in that In step S1, obtaining the crushed and ground polygonatum sibiricum comprises: Use fresh polygonatum or dried polygonatum as raw material, wash with 2-20 times water to remove foreign matter; The cleaned raw materials are added into a grinder and crushed into 30-300 mesh particles, and then 2-20 times of water is added to pass through a colloid mill and ground into 2-50 micron particles, and then heated to 85-100° C. and kept warm for 15-30 minutes for sterilization to obtain the crushed and ground Polygonatum sibiricum.
6. The preparation method according to claim 1, characterized in that In step S5, the secondary fermentation liquid is clarified to obtain a clarified liquid, comprising: Add 0.5-3% of curdlan gum, taro gum and cassia gum to the secondary fermentation liquid, with the ratio of curdlan gum, taro gum and cassia gum being 1:1-4:1-5, let it stand at room temperature for 2-8 hours, and take the supernatant as the clarified liquid.
7. The preparation method according to claim 1, characterized in that Step S6, decolorizing the clarified liquid to obtain a decolorized liquid, comprising: Add 0.5-5% activated clay to the clarified liquid, control the temperature at 55-65°C, decolorize for 30-90 minutes, and then filter by plate and frame to obtain the decolorized liquid.
8. The preparation method according to claim 1, characterized in that In step S7, the decolorized liquid is refined to obtain a polygonatum extract, comprising: The decolorized liquid is concentrated and then spray-dried to obtain the polygonatum sibiricum extract.
9. A product containing the polygonatum sibiricum extract prepared by the preparation method according to any one of claims 1 to 8.
10. Use of the product according to claim 9 in anti-aging.