Egg taking auxiliary device for reproductive medicine

Abstract

The invention discloses an ovum taking auxiliary device for reproductive medicine, relates to the technical field of medical ovum taking, and aims to solve the technical problem that cold shock is easy to occur in a transfer process. According to the ovum taking auxiliary device for reproductive medicine, ovum activity and transfer safety are guaranteed through multiple designs; the temperature fluctuation in the transfer window period is maintained to be smaller than or equal to + / -0.5 DEG C through a 37 DEG C phase change heat preservation material and a triple sealing and heat insulation structure, and the risk of cold shock is avoided; the rotational flow mechanism and the buffer film layer reduce mechanical damage to the ovum, and the collision probability is greatly reduced; the pulse-type split-flow conveying avoids water flow intersection, and zero damage to conveying is achieved; the memory alloy opening and closing plate is automatically opened in the cultivation box, and secondary transfer is omitted; an inert coating, a positioning column, a one-way valve and the like ensure that the microenvironment is clean and stable; the pain point of the traditional process is integrally solved, and a foundation is laid for ovum fertilization and embryonic development.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of medical oocyte retrieval, more particularly to an oocyte retrieval auxiliary device for reproductive medicine. BACKGROUND

[0002] In the oocyte retrieval surgery of reproductive medicine, the maintenance of oocyte activity is the core prerequisite for ensuring the success rate of assisted reproduction. The temperature stability and transfer efficiency of the follicular fluid in the 10-60 second key window period from puncture extraction to transfer to the constant temperature incubator directly affect the quality of oocytes. The traditional transfer process currently used in clinical practice has significant defects due to the lack of targeted temperature control and operation optimization design, which seriously threatens the activity of oocytes.

[0003] In the existing transfer process, the follicular fluid is extracted through a common plastic collection tube and then manually transferred to a constant temperature incubator (37℃) in a room temperature environment (22-25℃). Due to the high heat conduction efficiency of the common collection tube and the absence of any heat preservation structure, the temperature of the follicular fluid will rapidly decrease with exposure time - about 0.5-1℃ per 10 seconds. If the transfer time exceeds 30 seconds, the liquid temperature can drop to below 34℃, forming a temperature difference of more than 3℃ with the human body temperature (37℃). As oocytes are extremely sensitive to temperature, this rapid drop can cause a "cold shock" risk: on the one hand, the spindle of the metaphase oocyte (a key structure for maintaining chromosome separation) will be damaged due to low temperature, leading to spindle disassembly, which directly affects the normal separation of chromosomes; on the other hand, 37℃ is the optimal activity temperature for oocyte energy metabolism-related enzymes, and low temperature will inhibit enzyme activity, resulting in insufficient ATP generation, which will cause a rapid decrease in oocyte metabolic rate and a significant decrease in subsequent fertilization capacity and embryonic development potential.

[0004] The core reasons for this problem are twofold: first, the lack of an instant heat preservation device, the existing collection tube is made of single plastic material, which cannot block the rapid loss of heat, and there is no special constant temperature transfer equipment during the transfer process, which completely relies on the room temperature environment; second, the operation connection has an uncontrollable delay, the distance between the operating table and the incubator (often more than 5 meters), the information checking during the transfer process, the opening and closing of the incubator door, etc., all of which can prolong the exposure time and further exacerbate the rapid temperature drop. With the continuous improvement of the utilization rate of oocytes in assisted reproductive technology, the temperature control defects in the traditional transfer process have become a key factor restricting the success rate of surgery. In view of this, we propose an oocyte retrieval auxiliary device for reproductive medicine. SUMMARY

[0005] The present application aims to provide an oocyte retrieval auxiliary device for reproductive medicine to solve the technical problem of cold shock during the process of collection and transfer.

[0006] To solve the above technical problems, the present application provides the following technical solutions: an oocyte collection auxiliary device for reproductive medicine, comprising an oocyte collector, an oocyte storage cavity for storing follicular fluid is arranged inside the oocyte collector, a partition ring is fixed at the top of the oocyte collector, a plurality of opening and closing plates are installed at the top of the partition ring, the outer walls of the plurality of opening and closing plates are tightly attached to each other and seal the inside of the oocyte storage cavity, a one-way valve port is installed on the outside of each of the plurality of opening and closing plates, a liquid infusion tube is connected to each of the plurality of one-way valve ports, a liquid guide shell is connected between one end of the plurality of liquid infusion tubes, and a liquid infusion port is arranged on the liquid guide shell. The oocyte collector is composed of a hard shell and a flexible inner layer, the hard shell is made of a hard material, the flexible inner layer is made of a flexible material, a heat preservation cavity is arranged between the hard shell and the flexible inner layer, and the heat preservation cavity is filled with a heat preservation substance.

[0007] Preferably, the heat preservation substance is a phase change heat preservation material, and a plurality of positioning columns are connected between the hard shell and the flexible inner layer.

[0008] Preferably, a cyclone mechanism is arranged inside the flexible inner layer, the cyclone mechanism comprises a flow guide ring, the flow guide ring is in the form of an annular structure, the flow guide ring is located on the upper half of the inner wall of the flexible inner layer, a plurality of flow guide fins are connected between the outer wall of the flow guide ring and the inner wall of the flexible inner layer, and the plurality of flow guide fins are in the form of an inclined annular array.

[0009] Preferably, the cyclone mechanism further comprises a plurality of buffer film layers, the plurality of buffer film layers are located on one side of the one-way valve port and are made of an elastic film material, the side surface of the buffer film layer is bonded to the inner wall center area of the opening and closing plate, the opening end of the buffer film layer extends into the inside of the flow guide ring, the thickness of the buffer film layer on the side opposite to the one-way valve port is smaller than the thickness of the side, and the side of the buffer film layer is thickened.

[0010] Preferably, the liquid guide shell comprises an upper shell plate and a lower shell plate, the outer peripheral side wall of the upper shell plate and the lower shell plate is open, a one-way shaft ring is installed on the outer periphery of the upper shell plate and the lower shell plate, a center shell is arranged at the inner periphery of the upper shell plate, the center shell is in communication with the liquid infusion port, a plurality of shunt tubes are connected to the outer periphery of the center shell, and one end of the shunt tube is in communication with the inside of the upper shell plate and the lower shell plate.

[0011] Preferably, the shunt tube is in the form of a curved arc structure, the direction of the curved arc of the shunt tube output is the same as the direction of the free rotation of the one-way shaft ring, a bifurcated flow passage is arranged between the inner periphery of the upper shell plate and the lower shell plate, the bifurcated flow passage comprises a plurality of inner rotating blocks fixed to the inner side wall of the one-way shaft ring, the plurality of inner rotating blocks are in the form of an annular array and are equidistantly distributed, a bent connecting rod is connected between the plurality of inner rotating blocks, and the inner periphery of the inner rotating block is attached to the end of the liquid infusion tube.

[0012] Preferably, the plurality of inner rotating blocks are staggered at the output port position of the infusion tube, and the inner rotating blocks can open and close the infusion tube when rotating.

[0013] Preferably, the opening and closing plate comprises an opening and closing curved arc segment, the opening and closing curved arc segment is made of a material capable of being deformed by heat, the opening and closing curved arc segment is in a curved arc structure, and a pointed sealing segment extends from one side of the opening and closing curved arc segment.

[0014] Preferably, the outer circumferential side wall of the opening and closing plate is bonded with a side sealing layer, and a sealing protrusion is fixed to the pointed end of the opening and closing plate, and the opening and closing plate and the sealing protrusion are both made of a flexible material.

[0015] Preferably, a gas inlet head for inputting carbon dioxide is mounted on the oocyte extractor, and a one-way valve is arranged on the gas inlet head.

[0016] Compared with the prior art, the present application has the following advantages: 1. The present application maintains a 37 DEG C constant temperature environment for follicular fluid through multiple designs, the heat preservation cavity is filled with polyethylene glycol 4000 with a phase change temperature of 37 DEG C, and heat is continuously released during transfer, so that the temperature fluctuation is less than or equal to ± 0.5 DEG C within the 10-60 second window period, thereby avoiding temperature difference caused by traditional transfer; at the same time, the oocyte storage cavity is completely closed by the triple barriers of the opening and closing plate, i.e. side sealing, end sealing and plate body fitting, the temperature interference from the outside is blocked by the temperature isolation material of the separation ring, and the physiological microenvironment of the human body is simulated by cooperating with the pre-charged carbon dioxide gas, so as to prevent cold shock problems such as spindle depolymerization and enzyme activity inhibition caused by low temperature, and to protect the metabolism and fertilization potential of oocytes, thereby solving the problem of cold shock easily occurring in the process of transfer.

[0017] 2. The present application also forms double protection of "buffering-flow guiding" through the rotating flow mechanism of the flexible inner layer, the follicular fluid is first injected through the elastic buffer film layer, the structure of thin on one side and thick on the side is offset by flexible deformation to avoid direct impact of high-speed water flow; after buffering, the liquid is guided by the inclined annular array of flow guide plates to form a rotating flow along the cavity wall, thereby reducing the linear flow rate, and the centrifugal force makes the liquid uniformly distributed to reduce the collision between the oocyte and the cavity wall and between the oocytes; the two cooperate to reduce the collision probability, effectively protect the oocyte from mechanical damage, and maintain the active state.

[0018] 3. The liquid guiding shell of the present application realizes low-impact delivery through the arc-shaped shunt pipe and the staggered opening and closing structure, the central shell splits the single water flow into multiple small flow water flows, and the arc-shaped pipe disperses the impact force; the inner rotating block of the bifurcated flow member rotates directionally with the one-way shaft ring, the staggered shielding infusion tube port makes the follicular fluid output in a "pulse" rhythm, and each infusion tube presents a "short-term conduction-short-term closing" cycle; this design avoids the collision of water flow caused by the simultaneous output of adjacent pipes, the pulse interval period can also buffer the flow rate fluctuation, simulate the physiological flow characteristics, reduce the influence of continuous pressure on the oocyte, and realize zero mechanical damage in the delivery stage.

[0019] 4、The opening and closing plate adopts a thermal sensitive material triggered by deformation at 37 DEG C, and the external environment is isolated through multiple sealing structures during transfer to ensure the cleanliness and constant temperature of the follicular fluid; after entering the 37 DEG C incubator, the opening and closing arc segment is automatically stretched, the sealing segment is separated, the storage egg inner cavity is seamlessly connected with the incubation environment, and the incubation dish can be replaced without manual operation; the design eliminates the secondary operation of the traditional "transfer to the incubation dish", avoids the environmental contact risk and mechanical damage in the secondary transfer, and the temperature insulation property of the separation ring protects the temperature in the transfer process, and the convenience and protection are considered.

[0020] 5、The inner wall of the storage egg inner cavity is plated with a polytetrafluoroethylene inert coating to reduce the adhesion and residual loss of the follicular fluid; the positioning column between the hard shell and the flexible inner layer limits excessive deformation to ensure the volume stability and avoid the squeezing of the egg; the gas injection head injects carbon dioxide to form a physiological gas atmosphere, and the one-way valve prevents the backflow of liquid and gas; the multiple sealing structures block the entry of external pollutants and air to ensure that the follicular fluid is in a clean and closed environment during the transfer process; these designs ensure the stability of the egg microenvironment from the aspects of adhesion, deformation and pollution, and further reduce the influence of non-temperature factors on the activity of the egg. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 is a structural schematic diagram of the present application; Figure 2 is a half cut structural schematic diagram of the lower half part in the present application; Figure 3 is a half cut structural schematic diagram of the ovum extractor in the present application; Figure 4 is a structural schematic diagram of the opening and closing plate in the present application; Figure 5 is a structural schematic diagram of the outer peripheral sealing part of the opening and closing plate in the present application; Figure 6 is a structural schematic diagram of the buffer film layer in the present application; Figure 7 is a structural schematic diagram of the upper half part in the present application; Figure 8 is a structural schematic diagram of the opening and closing plate in the present application during the opening process; Figure 9 is an exploded structural schematic diagram of the liquid guide shell in the present application; Figure 10 is a structural schematic diagram of the bifurcated flow passage in the present application.

[0022] Explanation of reference numerals in the drawing: 1, take the egg device; 2, store the egg inner cavity; 3, separate ring; 4, open and close plate; 5, one-way valve port; 6, infusion tube; 7, liquid guide shell; 8, infusion port; 9, cyclone mechanism; 10, side sealing layer; 11, sealing convex head; 12, gas delivery head; 81, bending point; 101, hard shell; 102, flexible inner layer; 103, heat preservation cavity; 104, positioning column; 108, heat preservation material; 401, open and close bending arc segment; 402, sealing segment; 701, upper shell plate; 702, lower shell plate; 703, one-way collar; 704, center shell; 705, shunt tube; 76, bifurcated flow-through piece; 761, inner rotating block; 762, bent connecting rod; 901, flow guide ring; 902, flow guide fin; 903, buffer film layer. DETAILED DESCRIPTION

[0023] As Figures 1 to 10 shown, the application relates to a reproductive medicine egg retrieval auxiliary device, which comprises an egg retrieval device 1, an egg storage inner cavity 2 for storing follicular fluid is arranged in the egg retrieval device 1, the cavity inner wall is coated with an inert coating (polytetrafluoroethylene) to reduce the adhesion of follicular fluid, a separation ring 3 is fixed to the top of the egg retrieval device 1, a plurality of open and close plates 4 are installed on the top of the separation ring 3, the outer walls of the plurality of open and close plates 4 are tightly attached to each other and seal the inside of the egg storage inner cavity 2, the sealing can produce an isolated effect when the follicular fluid is extracted, so as to avoid external influence, a gas delivery head 12 for inputting carbon dioxide is installed on the egg retrieval device 1, a one-way valve is arranged on the gas delivery head 12, the gas delivery head 12 can fill an appropriate amount of carbon dioxide gas in the inside of the egg storage inner cavity 2 to ensure that an excellent cultivation environment is reached in a short time, a one-way valve port 5 is installed on the outer side of each of the plurality of open and close plates 4, an infusion tube 6 is connected to each of the plurality of one-way valve ports 5, a liquid guide shell 7 is connected between one end of the plurality of infusion tubes 6, an infusion port 8 is arranged on the liquid guide shell 7, the infusion port 8 is rigidly connected to the tail end of an egg retrieval needle through a Luer lock connector (compatible with a 17-18G egg retrieval needle), a one-way valve is arranged in the connector to prevent liquid backflow.

[0024] The egg retrieval device 1 is composed of a hard shell 101 and a flexible inner layer 102, a plurality of positioning columns 104 are connected between the hard shell 101 and the flexible inner layer 102, the positioning columns 104 have the effect of positioning the flexible inner layer 102 to avoid excessive internal deformation, the hard shell 101 is made of a hard material, the flexible inner layer 102 is made of a flexible material, a heat preservation cavity 103 is arranged between the hard shell 101 and the flexible inner layer 102, a heat preservation material 108 is filled in the heat preservation cavity 103, the heat preservation material 108 is a phase change heat preservation material, the phase change heat preservation material is preferably polyethylene glycol 4000, and the phase change temperature is 37 DEG C.

[0025] Working principle: Before the oocyte operation, the gas inlet head 12 is used to inject a proper amount of carbon dioxide gas into the oocyte inner cavity 2 (one-way valve prevents gas backflow), so that the inner cavity forms a gas atmosphere similar to the human physiological environment, providing the required gas conditions for short-term cultivation of oocytes; at the same time, the phase change heat preservation material (polyethylene glycol 4000, phase change temperature 37℃) filled in the heat preservation cavity 103 is preheated to a liquefied state, and its phase change characteristics can continuously release heat during the subsequent transfer process, maintaining the temperature of the oocyte inner cavity 2 at about 37℃, avoiding the "cold shock" of the follicular fluid caused by sudden temperature drop.

[0026] When oocytes are taken, the infusion port 8 is rigidly connected to the tail end of the 17-18G oocyte extraction needle through the luer lock connector (the interface is provided with a one-way valve to prevent backflow), and the follicular fluid extracted by the external oocyte extraction device enters the liquid guide shell 7 through the infusion port 8, and then enters the oocyte inner cavity 2 through the infusion tube 6 and the one-way valve port 5 (to prevent liquid backflow). During this process, the outer walls of the multiple opening and closing plates 4 at the top of the oocyte extractor 1 are tightly attached to each other, cooperating with the side sealing layer 10 and the sealing protrusion 11 to achieve complete sealing of the oocyte inner cavity 2, isolate the external room temperature environment (22-25℃) from the inner cavity, and prevent external pollutants from entering, ensuring that the follicular fluid is in a clean and isolated state.

[0027] The polytetrafluoroethylene inert coating on the inner wall of the oocyte inner cavity 2 can reduce the adhesion of the follicular fluid to the cavity wall and reduce the loss of oocytes caused by liquid residue; the positioning column 104 between the hard shell 101 and the flexible inner layer 102 limits the excessive deformation of the flexible inner layer 102, ensures the stability of the volume of the oocyte inner cavity 2, and avoids the influence of follicular fluid caused by cavity wall deformation on the shape of oocytes.

[0028] During the transfer of the follicular fluid to the constant temperature incubator, the phase change heat preservation material in a liquefied state continuously provides a 37℃ constant temperature environment for the oocyte inner cavity 2 through the heat preservation cavity 103, offsets the heat absorption of the external environment, and ensures that the temperature fluctuation of the follicular fluid during the transfer window period (10-60 seconds) does not exceed ±0.5℃; the sealed oocyte inner cavity 2 cooperates with the pre-filled carbon dioxide gas inside to further maintain the stability of the microenvironment required for oocyte metabolism, effectively avoiding the problem of decreased oocyte activity caused by sudden temperature drop and environmental exposure in the traditional transfer process, laying a foundation for subsequent fertilization and embryonic development.

[0029] During the output process, the oocytes in the follicular fluid are easily affected by high flow rate and other factors, causing mutual collision between the oocytes, resulting in mechanical damage. In order to protect the oocytes during the output process, the following structure is designed for this point.

[0030] The flexible inner layer 102 is internally provided with a cyclone mechanism 9, which comprises a flow guide ring 901, which is annular in structure as a whole, and is matched with the flexible inner layer 102, and a plurality of flow guide fins 902 are connected between the outer periphery of the flow guide ring 901 and the inner wall of the flexible inner layer 102, and the plurality of flow guide fins 902 are arranged in an inclined annular array.

[0031] The cyclone mechanism 9 further comprises a plurality of buffer film layers 903, which correspond to the positions of the one-way valve ports 5 and are made of elastic film material, the side surfaces of the buffer film layers 903 are bonded to the inner wall center area of the opening and closing plate 4, the opening ends of the buffer film layers 903 extend into the interior of the flow guide ring 901, the thickness of the buffer film layers 903 on the side opposite to the one-way valve ports 5 is smaller than the side thickness, and the side of the buffer film layers 903 is thickened.

[0032] Working principle: when the follicular fluid is injected into the ovum storage cavity 2 through the one-way valve port 5, it first contacts the buffer film layer 903 corresponding to the position of the one-way valve port 5. Since the buffer film layer 903 is made of elastic film material, and the thickness on the side opposite to the one-way valve port 5 is smaller than the side thickness (the side is thickened to enhance the structural stability), the input impact force of the follicular fluid will cause flexible deformation on the opposite side of the film. This elastic deformation can directly offset the water flow impact force in the initial input, avoiding the direct impact of high-speed water flow on the wall of the ovum storage cavity 2 or the ovum, playing a "first heavy buffer protection" role, and the elastic reset feature can prevent the risk of rupture caused by excessive deformation of the film.

[0033] After being buffered by the buffer film layer 903, the follicular fluid enters the interior of the flow guide ring 901 through the opening end of the film. The flow guide ring 901 is matched with the flexible inner layer 102, and the plurality of flow guide fins 902 connected between the outer periphery of the flow guide ring 901 and the inner wall of the flexible inner layer 102 are arranged in an inclined annular array, forming a directional flow guide channel. Under the guidance of the flow guide fins 902, the follicular fluid flows along an inclined trajectory and gradually converges, and finally forms a cyclone rotating along the inner wall of the ovum storage cavity 2. This cyclone pattern can convert the straight-line input flow rate of the follicular fluid into spiral flow, on the one hand, prolonging the water flow path and reducing the local flow rate per unit time (flow rate reduced by 30%-40%), reducing the water flow to the ovum; on the other hand, the centrifugal force generated by the cyclone makes the follicular fluid uniformly distributed in the peripheral area of the ovum storage cavity 2, avoiding the problem of ovum collision and accumulation caused by concentrated liquid impact on a certain area, achieving "second heavy directional protection".

[0034] In addition, the elastic deformation of the buffer film layer 903 cooperates with the directional flow guiding of the guide vane 902: the buffer film layer 903 adjusts the instantaneous pressure of the water flow input by deformation to avoid sudden changes in flow rate caused by sudden pressure rises; the guide vane 902 guides the water flow to form a stable vortex through the inclination angle (preferably 15°-20°), and under the combined action of the two, the collision probability in the follicular fluid input process is reduced by more than 60%, the flow rate fluctuation is controlled within ±5 mL / min, effectively protecting the eggs in the follicular fluid from mechanical damage and maintaining their active state.

[0035] In order to further protect the eggs, the liquid guide shell 7 is further designed in structure, as follows.

[0036] The liquid guide shell 7 includes an upper shell plate 701 and a lower shell plate 702, the outer peripheral side wall of the upper shell plate 701 and the lower shell plate 702 is open, a one-way collar 703 is installed on the outer periphery of the upper shell plate 701 and the lower shell plate 702, a central shell 704 is arranged at the inner periphery of the upper shell plate 701, the central shell 704 is in communication with the liquid inlet 8, a plurality of shunt pipes 705 are connected to the outer periphery of the central shell 704, and one end of the shunt pipe 705 is in communication with the inside of the upper shell plate 701 and the lower shell plate 702.

[0037] The shunt pipe 705 is a curved arc structure, the output bending radius of the shunt pipe 705 corresponds to the direction of free rotation of the one-way collar 703, a bifurcated flow passage 76 is arranged between the inner peripheries of the upper shell plate 701 and the lower shell plate 702, the bifurcated flow passage 76 includes a plurality of inner rotating blocks 761 fixed to the inner side wall of the one-way collar 703, the design of the one-way collar 703 allows the bifurcated flow passage 76 to rotate in only one direction when impacted, the plurality of inner rotating blocks 761 are arranged in a ring array at equal distances, a bent connecting rod 762 is connected between the plurality of inner rotating blocks 761, the outer periphery of the inner rotating block 761 is attached to the port of the liquid inlet pipe 6, the plurality of inner rotating blocks 761 are staggered and arranged at the output port position of the liquid inlet pipe 6, the staggered arrangement can synchronize the opening and closing of the relative liquid inlet pipe 6, avoid the opening of adjacent liquid inlet pipes 6, and reduce the possibility of collision during output, and the inner rotating block 761 can gap the relative position of the liquid inlet pipe 6 when rotating.

[0038] Working principle: follicular fluid enters the central shell 704 through the liquid inlet 8 (with a one-way valve to prevent backflow), is first divided by the plurality of arc-shaped shunt pipes 705 connected to the outer periphery of the central shell 704, the shunt pipe 705 adopts a curved arc structure, the output bending radius thereof corresponds to the direction of free rotation of the one-way collar 703, and the follicular fluid can gradually disperse the water flow impact force when flowing in the arc-shaped pipe, thereby avoiding sudden flow rate rise caused by straight-line delivery; at the same time, the design of the plurality of shunt pipes 705 divides the single water flow into a plurality of small flow water flows, thereby reducing the instantaneous impact force of each water flow and laying a "low-impact foundation" for subsequent pulse output.

[0039] After the follicular fluid is shunted into the cavity formed by the upper shell plate 701 and the lower shell plate 702, it acts on the inner rotating blocks 761 of the bifurcated flow passage 76. Since the inner rotating blocks 761 are fixed to the inner wall of the one-way shaft ring 703, and the one-way shaft ring 703 only allows rotation in a single direction, the impact of the follicular fluid will drive the inner rotating blocks 761 to rotate directionally with the one-way shaft ring 703. Since the multiple inner rotating blocks 761 are arranged in a ring array at equal distances and staggered corresponding to the output ports of the infusion tubes 6, the inner rotating blocks 761 will intermittently block or open the output ports of the infusion tubes 6 during rotation. When a group of inner rotating blocks 761 moves away from a certain output port of the infusion tube 6, the infusion tube 6 is opened and follicular fluid is delivered. At the same time, the adjacent output port of the infusion tube 6 is blocked by another group of inner rotating blocks 761, achieving "staggered opening and closing" and avoiding the collision of water flow caused by the simultaneous output of adjacent infusion tubes 6.

[0040] This staggered opening and closing control method allows multiple streams of follicular fluid to enter the infusion tubes 6 in a "pulse-like" rhythm: each infusion tube 6 is in a cycle state of "short-term conduction-short-term closure", and the output follicular fluid presents an intermittent, low-flow pulse form. Pulsatile flow can avoid the long-term impact of follicular fluid on oocytes at a single speed and pressure, reduce the continuous pressure and damage to oocytes, and the simulation of physiological flow characteristics may be more suitable for the natural living environment of oocytes, helping to maintain the activity and function of oocytes. At the same time, the pulse interval period allows the follicular fluid to be temporarily buffered in the pipeline, further reducing flow rate fluctuations and reducing the probability of collisions between oocytes and the pipeline wall and between oocytes during delivery, ultimately achieving the protection effect of "zero mechanical damage in the delivery stage" and providing high-quality follicular fluid for subsequent constant-temperature preservation of the oocyte storage lumen 2.

[0041] In order to further improve the convenience of use and reduce the influence of the external environment, a structure design that can be automatically opened in the cultivation environment is added here. The specific structure is as follows.

[0042] The partition ring 3 is made of a poor heat-conducting temperature insulation material, and the opening and closing plate 4 includes an opening and closing curved arc segment 401 made of a heat-deformable material. The heat-deformable material is selected to be a material that can automatically deform at 37 degrees (preferably Ti-Ni-Cu ternary alloy in SMA), and the opening and closing curved arc segment 401 has a curved arc structure with a sharp conical sealing segment 402 extending from one side.

[0043] The center of the opening and closing curved arc segment 401 is provided with a bending point 81, as shown in Figure 4 When multiple opening and closing plates 4 are closed, the bending point 81 is in a force-free balanced state. When the environment is at 37 degrees, the opening and closing curved arc segment 401 is bent under stress, as shown in Figure 8As shown, the bending point 81 is in a state of moving outward, the separation ring 3 is made of a poor heat-conducting temperature insulation material, which can block the influence of the external environment temperature on the egg storage cavity 2, so that only the egg extractor 1 needs to be heated when heated, and the heat of the heating does not affect the opening and closing plate 4 part, so as to maintain the sealing state.

[0044] In order to strengthen the sealing effect, the outer peripheral side wall of the opening and closing plate 4 is bonded with a side sealing layer 10, and the pointed end of the opening and closing plate 4 is fixed with a sealing protrusion 11, and the opening and closing plate 4 and the sealing protrusion 11 are both made of flexible material.

[0045] Working principle: in the follicular fluid extraction and transfer stage, the separation ring 3 is made of a poor heat-conducting temperature insulation material, which can block the influence of the external environment temperature on the egg storage cavity 2, cooperate with the 37℃ phase change heat preservation material in the heat preservation cavity 103, maintain the constant temperature of the inner cavity; at the same time, the opening and closing plate 4 realizes the complete isolation of the egg storage cavity 2 through multiple sealing structures: the side sealing layer 10 of the outer peripheral side wall of the opening and closing plate 4 fills the gap between the plate bodies, the sealing protrusion 11 (both the opening and closing plate 4 are flexible materials) at the end of the pointed sealing section 402 is tightly fitted, and the outer walls of multiple opening and closing plates 4 are tightly fitted with each other, forming a "side sealing + end sealing + plate body fitting" three-seal barrier, effectively isolating the external air, pollutants and temperature fluctuations, ensuring that the follicular fluid is in a clean, constant temperature closed environment during the transfer process, avoiding the influence of activity caused by contact with the external environment.

[0046] After the infusion tube 6 is pulled out and the device is transferred to the incubator / constant temperature box (the internal temperature is maintained at 37℃), the opening and closing bending arc section 401 of the opening and closing plate 4 plays a temperature control deformation role: because it is made of a heat-sensitive material that triggers deformation at 37℃, in the constant temperature environment of the incubator, the opening and closing bending arc section 401 will automatically undergo directional deformation, the plate body of the bending arc structure will stretch along the curvature direction, and the pointed sealing section 402 will separate outward. With the continuous deformation, the opening and closing plates 4 that were originally in close contact are gradually opened, and the egg storage cavity 2 directly borders the incubation environment (such as a specific gas atmosphere, a constant temperature environment) inside the incubator. At this time, the device can be directly used instead of the incubation dish, without the need to transfer the follicular fluid to other containers.

[0047] The whole process does not need manual operation, and the seamless connection of "sealing and isolation during transfer-automatic opening during incubation" is realized through the temperature control characteristics of the material: on the one hand, it avoids the "taking out follicular fluid-transferring to incubation dish" secondary operation in the traditional process, reduces the risk of contact between the ova and the external environment during the transfer process, and the possible collision and damage of the ova caused by mechanical transfer; on the other hand, the temperature insulation property of the separation ring 3 protects the temperature of the inner cavity during the transfer process, and the automatic opening of the opening and closing plate 4 ensures rapid adaptation after entering the incubation environment, taking into account convenience and protection, providing stable and continuous environmental support for ova incubation.

[0048] The embodiments of the present application are disclosed above, but not limited to, the preferred embodiments, and those skilled in the art can make different deductions and changes according to the above embodiments, and the different deductions and changes should be within the protection scope of the present application as long as they do not deviate from the spirit of the present application.

Claims

1. An egg retrieval auxiliary device for reproductive medicine, characterized in that: The invention comprises an egg retrieval device (1), wherein the interior of the egg retrieval device (1) is configured as an egg storage cavity (2) for storing follicular fluid, a separation ring (3) is fixed on the top of the egg retrieval device (1), a plurality of opening and closing plates (4) are installed on the top of the separation ring (3), the outer walls of the plurality of opening and closing plates (4) are closely attached to each other and seal the interior of the egg storage cavity (2), a one-way valve port (5) is installed on the outer side of the plurality of opening and closing plates (4), the plurality of one-way valve ports (5) are connected to an infusion tube (6), a liquid guide shell (7) is connected between one ends of the plurality of infusion tubes (6), and an infusion port (8) is provided on the liquid guide shell (7); The egg retrieval device (1) is composed of a hard outer shell (101) and a flexible inner layer (102), wherein the hard outer shell (101) is made of a hard material, and the flexible inner layer (102) is made of a flexible material. A heat preservation cavity (103) is provided between the hard outer shell (101) and the flexible inner layer (102), and the heat preservation cavity (103) is filled with a heat preservation material (108).

2. The egg retrieval auxiliary device for reproductive medicine according to claim 1, characterized in that: The heat-insulating substance (108) is a phase-change heat-insulating material, and a plurality of positioning posts (104) are connected between the hard outer shell (101) and the flexible inner layer (102).

3. The egg retrieval auxiliary device for reproductive medicine according to claim 2, characterized in that: A swirl mechanism (9) is provided inside the flexible inner layer (102), and the swirl mechanism (9) comprises a guide ring (901), the guide ring (901) being an annular structure as a whole, the guide ring (901) being located at the upper half of the inner circumference of the flexible inner layer (102), a plurality of guide vanes (902) being connected between the outer circumference of the guide ring (901) and the inner wall of the flexible inner layer (102), and the plurality of guide vanes (902) being distributed in an inclined annular array.

4. The egg retrieval auxiliary device for reproductive medicine according to claim 3, characterized in that: The swirl mechanism (9) further comprises a plurality of buffer film layers (903), the plurality of buffer film layers (903) being located on one side of the one-way valve port (5) and being made of an elastic film material, the side of the buffer film layer (903) being bonded to the central area of ​​the inner wall of the opening and closing plate (4), the open end of the buffer film layer (903) extending into the interior of the guide ring (901), the thickness of the side of the buffer film layer (903) facing the one-way valve port (5) being smaller than the thickness of the side, and the side of the buffer film layer (903) being thickened.

5. The egg retrieval auxiliary device for reproductive medicine according to claim 4, characterized in that: The liquid-conducting housing (7) comprises an upper shell plate (701) and a lower shell plate (702), the outer peripheral side walls of the upper shell plate (701) and the lower shell plate (702) being open, and a one-way shaft ring (703) being installed on the outer periphery of the upper shell plate (701) and the lower shell plate (702), and a central shell (704) being provided at the inner center of the upper shell plate (701), the central shell (704) being in communication with the infusion port (8), and a plurality of shunt tubes (705) being connected to the outer periphery of the central shell (704), and one end of the shunt tubes (705) being in communication with the interior of the upper shell plate (701) and the lower shell plate (702).

6. The egg retrieval auxiliary device for reproductive medicine according to claim 5, characterized in that: The shunt tube (705) is a curved arc structure. The direction of the output bending arc of the shunt tube (705) is the same as the direction of free rotation of the one-way shaft ring (703). A bifurcated flow member (76) is provided between the inner peripheries of the upper shell plate (701) and the lower shell plate (702). The bifurcated flow member (76) includes a plurality of inward rotating blocks (761) fixed to the inner side wall of the one-way shaft ring (703). The plurality of inward rotating blocks (761) are equidistantly distributed in a circular array. A curved connecting rod (762) is connected between the plurality of inward rotating blocks (761). The outer periphery of the inward rotating block (761) is in contact with the port of the infusion tube (6).

7. The egg retrieval auxiliary device for reproductive medicine according to claim 6, characterized in that: The plurality of inner rotating blocks (761) are staggeredly distributed at the output port position of the infusion tube (6), and the inner rotating blocks (761) can open and close the infusion tube (6) when rotating.

8. The egg retrieval auxiliary device for reproductive medicine according to any one of claims 4 to 7, characterized in that: The separation ring (3) is made of a heat-insulating material with poor thermal conductivity. The opening and closing plate (4) includes an opening and closing curved arc section (401). The opening and closing curved arc section (401) is made of a material that deforms when heated. The opening and closing curved arc section (401) is a curved arc structure. A pointed conical sealing section (402) extends from one side of the opening and closing curved arc section (401).

9. The egg retrieval auxiliary device for reproductive medicine according to claim 8, characterized in that: The outer peripheral side wall of the opening and closing plate (4) is bonded with a side sealing layer (10), and the pointed conical end of the opening and closing plate (4) is fixed with a sealing protrusion (11). The opening and closing plate (4) and the sealing protrusion (11) are both made of flexible material.

10. The egg retrieval auxiliary device for reproductive medicine according to claim 9, characterized in that: The egg retrieval device (1) is provided with a gas supply head (12) for supplying carbon dioxide, and a one-way valve is provided on the gas supply head (12).

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