Device integrating cell culture and fluorescence monitoring
By embedding a fluorescence signal imaging device in the cell culture incubator and combining it with a temperature and carbon dioxide control system, the problem of environmental interference between the cell culture incubator and the fluorescence photography process was solved, cell dynamic monitoring and real-time data transmission were achieved, and the integrity and safety of the experiment were improved.
Patent Information
- Application Number
- CN202510974744.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-15
- Publication Date
- 2025-10-17
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
In the prior art, during the cell culture incubator and fluorescence photography process, the transfer of cells from the incubator to the fluorescence microscope is easily disturbed by the external environment, affecting the cell state and increasing contamination, which is especially obvious when observing dynamic changes at multiple time points.
A device integrating cell culture and fluorescence monitoring is designed. The fluorescence signal imaging device is embedded in the incubator. Combined with the temperature and carbon dioxide control system, the integration of cell culture and fluorescence monitoring is achieved. Dynamic photography is achieved through the X-axis and Y-axis moving mechanisms, and real-time data is transmitted to the computer terminal.
It effectively avoids external environmental interference, simplifies workflow, and enables real-time monitoring of cell dynamic growth trends and drug toxicity effects, ensuring the integrity and safety of the experiment.
Smart Images
Figure CN120796046A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of cell culture monitoring, in particular to a device integrating cell culture and fluorescence monitoring. BACKGROUND
[0002] In the process of cell culture, cell detection is often accompanied. In the prior art, a cell culture container is usually placed in a culture box for a certain period of time, and then taken out from the culture box for observation, photographing and analysis by a fluorescence microscope. The difficulty of the current cell culture box and fluorescence photographing is that the fluorescence photographing of the cells must be performed outside the culture box, and there is a manual transfer process from the culture box to the fluorescence photographing, which is easily disturbed by the environment outside the culture box, thereby affecting the state of the cells and increasing the pollution of the cells. If the dynamic changes of the cells in the culture box are to be observed, especially the dynamic changes at multiple time points, this situation will be more obvious. Therefore, there is an urgent need for a device integrating cell culture and fluorescence monitoring. SUMMARY
[0003] The main purpose of the present application is to provide a device integrating cell culture and fluorescence monitoring to solve the above problems.
[0004] To achieve the above purpose, the present application provides a device integrating cell culture and fluorescence monitoring, comprising a box body and a box door hinged to the box body; a glass storage plate is arranged in the box body, which divides the inner cavity of the box body into a culture cavity and a monitoring cavity; a door opening matched with the box door is formed in the front side of the culture cavity; a temperature sensor and a carbon dioxide sensor are arranged in the culture cavity, a carbon dioxide gas inlet pipe communicating with the culture cavity is arranged on the top of the box body, and an electromagnetic valve is arranged on the carbon dioxide gas inlet pipe; a heating mechanism is arranged in the side wall of the box body; an X-axis moving mechanism is fixedly arranged in the monitoring cavity, a Y-axis moving mechanism is arranged on the X-axis moving mechanism, and a fluorescence signal imaging device is arranged on the Y-axis moving mechanism.
[0005] Further, a temperature control panel and a carbon dioxide control panel are arranged on the outside of the box body, the heating mechanism and the temperature sensor are electrically connected with the temperature control panel, and the electromagnetic valve and the carbon dioxide sensor are electrically connected with the carbon dioxide control panel.
[0006] Further, the heating mechanism comprises a medium cavity and a heating pipe; the medium cavity is filled with a heating medium, and the heating pipe is arranged in the medium cavity; the medium cavity is located above the glass storage plate.
[0007] Further, a heat preservation layer is arranged on the outside of the box body.
[0008] Further, two sides of the monitoring cavity are respectively provided with an air inlet and an air outlet; the air inlet is provided with a filtering mechanism, and the air outlet is provided with an exhaust fan.
[0009] Further, the filtering mechanism comprises a fixed ring with a stopper, and a plurality of filter screens are arranged in the fixed ring; the fixed ring is slidingly inserted into the air inlet.
[0010] Further, the device further comprises a driving motor and a fan blade, the driving motor is installed at the rear of the box body, the fan blade is fixed on the output shaft of the driving motor, and the fan blade is located in the culture cavity.
[0011] Further, the X-axis moving mechanism and the Y-axis moving mechanism both adopt a linear slide module.
[0012] The present application has the following beneficial effects:
[0013] The present application embeds the fluorescence signal imaging device into the incubator, can realize cell culture and fluorescence monitoring at the same time, effectively avoids external environmental interference, and simplifies the working process. The time can be set according to the user's demand, and the cell is dynamically photographed to obtain the dynamic growth trend of the cell. In addition, all data can be transmitted in real time, and recorded on the computer terminal to ensure the integrity and safety of the experiment. BRIEF DESCRIPTION OF DRAWINGS
[0014] Fig. 1 It is a whole schematic view of the device for integrating cell culture and fluorescence monitoring.
[0015] Fig. 2 It is a sectional view of the device for integrating cell culture and fluorescence monitoring.
[0016] 1 - carbon dioxide inlet pipe; 2 - electromagnetic valve; 3 - box body; 4 - box door; 5 - temperature control panel; 6 - carbon dioxide control panel; 7 - heat preservation layer; 8 - culture cavity; 9 - fan blade; 10 - temperature sensor; 11 - carbon dioxide sensor; 12 - medium cavity; 13 - heating pipe; 14 - cell culture container; 15 - glass storage plate; 16 - fluorescence signal imaging device; 17 - Y-axis moving mechanism; 18 - X-axis moving mechanism; 19 - exhaust fan; 20 - air outlet; 21 - air inlet; 22 - fixed ring; 23 - plurality of filter screens; 24 - monitoring cavity. DETAILED DESCRIPTION
[0017] In order to achieve the above-mentioned purposes and effects, the technical means and structure adopted by the present application are described in detail in combination with the preferred embodiments of the present application, and the characteristics and functions are described in combination with the drawings.
[0018] For example, Figs. 1-2As shown, the present application provides a device integrating cell culture and fluorescence monitoring, comprising a box 3 and a box door 4 hinged to the box 3; a glass storage plate 15 is arranged in the box 3, which separates the inner cavity of the box into a culture cavity 8 and a monitoring cavity 24; a door opening adapted to the box door 4 is formed in the front side of the culture cavity 8; a temperature sensor 10 and a carbon dioxide sensor 11 are arranged in the culture cavity 8; a carbon dioxide inlet pipe 1 communicating with the culture cavity 8 is arranged on the top of the box 3, and an electromagnetic valve 2 is arranged on the carbon dioxide inlet pipe 1; a heating mechanism is arranged in the side wall of the box 3; an X-axis moving mechanism 18 is fixedly arranged in the monitoring cavity 24, an Y-axis moving mechanism 17 is arranged on the X-axis moving mechanism 18, and a fluorescence signal imaging device 16 is arranged on the Y-axis moving mechanism 17. The X-axis moving mechanism 18 and the Y-axis moving mechanism 17 both adopt linear slide table modules. A temperature control panel 5 and a carbon dioxide control panel 6 are arranged outside the box 3, and the heating mechanism and the temperature sensor 10 are electrically connected to the temperature control panel 5, and the electromagnetic valve 2 and the carbon dioxide sensor 11 are electrically connected to the carbon dioxide control panel 6.
[0019] In use, the box door 4 is opened, the transparent cell culture container 14 is placed on the glass storage plate 15, and then the box door 4 is closed; the temperature value and the carbon dioxide content value (such as 37℃, 5% CO2) are set through the temperature control panel 5 and the carbon dioxide control panel 6 respectively, when the temperature sensor 10 detects that the gas in the culture cavity 8 reaches the set temperature value, the heating mechanism stops heating, when the temperature sensor 10 detects that the gas in the culture cavity 8 is lower than the set temperature value, the heating mechanism heats, when the temperature sensor 10 detects that the gas in the culture cavity 8 reaches the set temperature, the heating mechanism stops heating; when the carbon dioxide sensor 11 detects that the carbon dioxide concentration in the culture cavity 8 is lower than the set carbon dioxide content value, the electromagnetic valve 2 is opened, the carbon dioxide inlet pipe 1 is connected to the carbon dioxide, when the carbon dioxide sensor 11 detects that the carbon dioxide concentration in the culture cavity 8 reaches the set carbon dioxide content value, the electromagnetic valve 2 is closed; the X-axis moving mechanism 18 and the Y-axis moving mechanism 17 are controlled to move through the computer terminal, so that the fluorescence signal imaging device 16 moves, the cells in the cell culture container 14 are monitored, and the data is transmitted to the computer terminal for saving and analyzing, so as to ensure the integrity and safety of the experiment. The user can set the time according to the needs, dynamically take the fluorescence photos of the cells, and obtain the dynamic growth trend of the cells.
[0020] In another embodiment, the heating mechanism comprises a medium cavity 12 and a heating pipe 13; the medium cavity 12 is filled with heating medium, and the heating pipe 13 is arranged in the medium cavity 12; the medium cavity 12 is located above the glass placement plate 15. The heating medium in the medium cavity 12 is heated by the heating pipe 13, and then the heat is transferred to the gas in the culture cavity 8 through the inner wall of the box 3.
[0021] In another embodiment, the box 3 is provided with a heat preservation layer 7. The heat preservation layer 7 prevents heat loss.
[0022] In another embodiment, the monitoring cavity 24 is provided with an air inlet 21 and an air outlet 20 on both sides; the air inlet 21 is provided with a filtering mechanism, and the air outlet 20 is provided with an exhaust fan 19. The filtering mechanism filters impurities in the external air, prevents impurities from entering the monitoring cavity 24 and polluting the components in the monitoring cavity 24, and the exhaust fan 19 discharges the heat emitted by the components in the monitoring cavity 24, ensuring the normal operation of the components.
[0023] In another embodiment, the filtering mechanism comprises a fixed ring 22 with a stopper, and the fixed ring 22 is provided with multiple layers of filter screens 23; the fixed ring 22 is slidingly inserted into the air inlet 21. The filtering mechanism is in a detachable form, so as to facilitate disassembly and replacement.
[0024] In another embodiment, a driving motor and a fan blade 9 are further included, the driving motor is installed at the rear of the box 3, the fan blade 9 is fixed on the output shaft of the driving motor, and the fan blade 9 is located in the culture cavity 8. The driving motor drives the fan blade 9 to rotate, and then the gas in the culture cavity 8 circulates and flows, so that the gas is uniformly heated, and the inhaled carbon dioxide is uniformly mixed with the air.
[0025] The above only describes the preferred embodiments of the present application, not all embodiments. Any structural changes made under the inspiration of the present application should be known, and any technical solutions similar to the present application should be within the protection scope of the present application.
Claims
1. A device integrating cell culture and fluorescence monitoring, characterized in that: The invention comprises a box body and a door hinged to the box body; a glass storage plate is provided in the box body, and the glass storage plate divides the inner cavity of the box body into a culture cavity and a monitoring cavity; a door adapted to the box door is provided on the front side of the culture cavity; a temperature sensor and a carbon dioxide sensor are provided in the culture cavity, a carbon dioxide inlet pipe communicating with the culture cavity is provided on the top of the box body, and a solenoid valve is provided on the carbon dioxide inlet pipe; a heating mechanism is provided in the side wall of the box body; an X-axis moving mechanism is fixedly provided in the monitoring cavity, a Y-axis moving mechanism is provided on the X-axis moving mechanism, and a fluorescence signal imaging device is provided on the Y-axis moving mechanism.
2. The device for integrating cell culture and fluorescence monitoring according to claim 1, characterized in that: A temperature control panel and a carbon dioxide control panel are provided on the outside of the box body. The heating mechanism and the temperature sensor are both electrically connected to the temperature control panel, and the solenoid valve and the carbon dioxide sensor are both electrically connected to the carbon dioxide control panel.
3. The device for integrating cell culture and fluorescence monitoring according to claim 1 or 2, characterized in that: The heating mechanism includes a medium cavity and a heating tube; the medium cavity is filled with a heating medium, and the heating tube is arranged in the medium cavity; the medium cavity is located above the glass storage plate.
4. The device for integrating cell culture and fluorescence monitoring according to claim 3, characterized in that: A heat-insulating layer is provided on the outside of the box.
5. The device for integrating cell culture and fluorescence monitoring according to claim 1 or 4, characterized in that: An air inlet and an air outlet are respectively provided on both sides of the monitoring cavity; a filtering mechanism is provided on the air inlet, and an exhaust fan is provided on the air outlet.
6. The device for integrating cell culture and fluorescence monitoring according to claim 5, characterized in that: The filtering mechanism comprises a fixing ring with a stop, wherein multiple layers of filtering screens are arranged in the fixing ring; the fixing ring is slidably inserted in the air inlet.
7. The device for integrating cell culture and fluorescence monitoring according to claim 1, characterized in that: It also includes a driving motor and fan blades. The driving motor is installed at the rear of the box body. The fan blades are fixed on the output shaft of the driving motor and are located in the culture chamber.
8. The device for integrating cell culture and fluorescence monitoring according to claim 1, wherein: The X-axis moving mechanism and the Y-axis moving mechanism both adopt linear slide modules.