Culture detection device for biological fermentation

By designing a detection device with a dual-test component for picking up and placing samples and a receiving and placing component, the problems of slow sample detection and long storage time in bio-fermentation sampling and detection are solved. This enables rapid isolation sampling and multiple detections of samples, improving detection efficiency and accuracy.

CN120796055AInactive Publication Date: 2025-10-17LANGFANG NORMAL UNIV
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Patent Information

Application Number
CN202511299244.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-09-12
Publication Date
2025-10-17
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

In existing biological fermentation sampling and testing, samples cannot be tested quickly and are stored for a long time, resulting in large deviations in test results and affecting test accuracy.

Method used

A detection device was designed, which includes a dual-test component for picking up and placing samples and a receiving and placing component. By integrating a constant temperature chamber with a fermenter, it uses components such as a centrifugal motor and a switching motor to achieve rapid isolation and sampling of samples and multiple tests. It is used in conjunction with a vacuum sampling box and a light detector for rapid detection.

Benefits of technology

It enables rapid isolated sampling and multiple detections of samples within the same device, reduces the impact of environmental changes on samples, improves detection efficiency and accuracy, reduces sample storage time, avoids sample mutation, and enhances detection stability.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention discloses a culture detection device for biological fermentation, and relates to the technical field of biological fermentation, a centrifugal motor is mounted on one side of the bottom end of an integrated constant-temperature box through a motor base, a centrifugal clamping frame is clamped to an output shaft of the centrifugal motor, and pressing electric push rods are symmetrically clamped to the positions, close to the centrifugal clamping frame, of the bottom end of the integrated constant-temperature box; a folding and unfolding motor is installed at the top end of the pressing electric push rod through a motor base, a folding and unfolding sealing cover is clamped to an output shaft of the folding and unfolding motor, correction electric push rods are symmetrically clamped to the middle of the bottom end of the integration constant-temperature box, a correction operation frame is clamped to the top ends of the two correction electric push rods, and lifting electric push rods are symmetrically installed at the top end of the correction operation frame; according to the invention, the equipment is used for directly and quickly treating the sample, so that the storage time of the sample is shortened, the situation that the sample is changed due to overlong storage time is avoided, the actual detection efficiency and the stability of the detection result are effectively improved, and the accuracy of the detection data is ensured.
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Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological fermentation, in particular to a culture detection device for biological fermentation. BACKGROUND

[0002] Biological fermentation is a process of converting organic substrates into target products or changing the properties of substances under controlled conditions by using the metabolic activities of microorganisms or enzymes. The substrate is usually sugar, and the product is the target substance produced by microbial metabolic activity, the microbial cells themselves, or the form of the substrate after conversion. Biological fermentation is usually carried out in specialized bioreactors, which require precise control of temperature, pH, dissolved oxygen, stirring speed, pressure, and nutrient concentration.

[0003] The patent with application number CN202221527143.4 mentions a "wax rib microorganism fermentation optimal temperature detection device". The patent rotates the screw valve cover, so that the piston wrapped outside the top end of the receiving seat slides backward and fits the leakage groove, thereby allowing the internal pressure of the fermentation box body to be exhausted through the communication of the leakage groove and the air pipe, without the need to repeatedly open the fermentation box body, ensuring the internal fermentation effect.

[0004] However, when sampling and detecting biological fermentation, the sampling position cannot be effectively isolated from the environment, and after sampling, the sample is easily exposed to the environment, causing the microorganisms or enzymes in the sample to die due to short-term environmental changes. In addition, the sample cannot be quickly detected, the sample storage time is long, and the sample sampling and testing results may deviate greatly, affecting the actual detection accuracy. SUMMARY

[0005] The present application provides a culture detection device for biological fermentation, which can effectively solve the problem of slow detection of samples, long storage time of samples, and large deviation of sampling and testing results, affecting the actual detection accuracy.

[0006] To achieve the above purpose, the present application provides the following technical scheme: a culture detection device for biological fermentation, comprising an integrated incubator, the integrated incubator is provided with a taking and placing double measurement assembly at the side end;

[0007] The taking and placing double measurement assembly comprises a centrifugal motor;

[0008] The centrifugal motor is installed on the bottom end of the integrated incubator through the motor seat, and the centrifugal motor output shaft is connected with a centrifugal clamping frame;

[0009] The integrated incubator is symmetrically connected with a compression electric push rod near the centrifugal clamping frame at the bottom end;

[0010] The pressing electric push rod top is installed in the take-up motor through the motor base, and the take-up motor output shaft is connected with the take-up sealing cover;

[0011] The integrated thermostat bottom middle part is symmetrically connected with the correction electric push rod, and the two correction electric push rod tops are connected with the correction operation frame;

[0012] The correction operation frame top is symmetrically installed with the lifting electric push rod, and the two lifting electric push rod tops are installed with the switching motor through the motor base;

[0013] The switching motor output shaft is connected with the switching operation frame, and the switching operation frame one end is installed with the swing motor through the motor base.

[0014] According to the above technical scheme, the swing motor output shaft is connected with the swing operation frame, and the swing operation frame inner side one end is installed with the transposition motor through the motor base;

[0015] The transposition motor output shaft is connected with the transposition integrated frame, and the transposition integrated frame side end is installed with the linkage motor through the motor base at equal intervals;

[0016] The linkage motor output shaft is connected with the transposition hollow block, and the transposition hollow block top is symmetrically installed with the clamping electric slide rail;

[0017] The clamping electric slide rail top is installed with the clamping operation block through the slide rail base;

[0018] The centrifugal clamping frame is rotatably installed in the integrated thermostat inner side, and the take-up sealing cover bottom is attached to the centrifugal clamping frame top.

[0019] According to the above technical scheme, the swing operation frame and the switching operation frame are rotatably combined, the transposition hollow block has six, and the transposition hollow block is rotatably installed in the transposition integrated frame inner side.

[0020] According to the above technical scheme, the integrated thermostat bottom is symmetrically installed with the alignment electric slide rail, and the alignment electric slide rail top is installed with the insertion operation frame through the slide rail base;

[0021] The insertion operation frame side end is symmetrically connected with the insertion electric push rod, and the insertion electric push rod one end is connected with the isolation detection box;

[0022] The isolation detection box inner side is installed with the light detector, and the isolation detection box bottom is connected with the attachment electric push rod.

[0023] According to the above technical scheme, the clamping operation block is slidably installed on the transposition hollow block top, the isolation detection box bottom is slidably attached to the insertion operation frame top, and the isolation detection box has two.

[0024] According to the technical scheme, the fitting operation frame is installed at one end of the fitting electric push rod, and a counterpart electric push rod is installed at the top end of the fitting operation frame.

[0025] The counterpart operation block is clamped at the bottom end of the counterpart electric push rod, and the exhaust treatment pipe is clamped at one end of the counterpart operation block.

[0026] The liquid pumping pump is installed at one side of the top end of the fitting operation frame through the motor base.

[0027] The liquid pumping pump is installed at one side of the top end of the fitting operation frame through the motor base.

[0028] According to the technical scheme, the liquid pumping pump is connected to one end of the exhaust treatment pipe through the adapter.

[0029] The input ends of the centrifugal motor, the pressing electric push rod, the retracting motor, the correcting electric push rod, the lifting electric push rod, the switching motor, the swinging motor, the transposition motor, the linkage motor, the clamping electric slide rail, the alignment electric slide rail, the inserting electric push rod, the light detector, the fitting electric push rod, the counterpart electric push rod and the liquid pumping pump are electrically connected to the output end of the external controller.

[0030] The input end of the external controller is electrically connected to the output end of the external power supply.

[0031] According to the technical scheme, the integrated incubator is provided with a retracting assembly at the side end.

[0032] The retracting assembly comprises an inserting electric push rod.

[0033] The inserting electric push rod is symmetrically installed at one side of one end of the integrated incubator, and a treatment inserting box is installed at one end of the inserting electric push rod.

[0034] The taking and placing electric slide rail is embedded at one end of the inner side of the treatment inserting box, and the combination electromagnet is installed at one end of the taking and placing electric slide rail through the slide rail base and the clamping connecting sleeve.

[0035] The taking and placing electric push rod is symmetrically installed at the other side of one end of the integrated incubator, and a taking and placing operation box is installed at one end of the taking and placing electric push rod.

[0036] The pushing electric slide rail is clamped at one end of the inner side of the taking and placing operation box, and the pushing operation plate is installed at one end of the pushing electric slide rail through the slide rail base.

[0037] The integrated incubator is provided with a plurality of bearing electric push rods at the side end, and the universal joint is clamped at one end of the bearing electric push rod.

[0038] The lower discharging motor is connected with the lower discharging screw rod through the screw rod seat.

[0039] According to the above technical scheme, the lower discharging screw rod is connected with the lower discharging operation frame through the screw rod seat.

[0040] The vacuum sampling box is connected with the integrated incubator through the integrated incubator.

[0041] The vacuum sampling box is connected with the integrated incubator through the integrated incubator.

[0042] The vacuum sampling box is connected with the integrated incubator through the integrated incubator.

[0043] The push material operation plate is slidably installed in the taking and placing operation box.

[0044] According to the above technical scheme, the lower discharging screw rod is connected with the lower discharging operation frame through the screw rod seat.

[0045] The input ends of the insertion and combination electric push rod, the taking and placing electric slide rail, the combined electromagnet, the taking and placing electric push rod, the push material electric slide rail, the bearing electric push rod, the lower discharging motor, the overturning motor, the electromagnetic valve and the extrusion electric push rod are electrically connected with the output end of the external controller.

[0046] Compared with the prior art, the present application has the following advantages:

[0047] 1. The taking and placing double measuring assembly is provided, the integrated incubator and the liquid injection operation box are directly connected with the fermentation tank and the sampling port through the integrated incubator and the liquid injection operation box, the quick sampling is realized through the snap-fit connecting sleeve and the vacuum sampling box, the isolated sampling is realized, the sample is prevented from being directly exposed to the environment, the switching operation frame, the swinging operation frame and the rotating and switching integrated frame are driven to rotate and switch through the switching motor, the switching motor is driven to lift through the correction electric push rod, the correction operation frame and the lifting electric push rod, the position of the centrifugal sealed test tube is adjusted, the direction of the linkage motor and the rotating and switching hollow block is changed, the stable operation of the taking and placing and the detection is realized, the sample can be quickly operated during different tests and sample taking and placing, and the efficiency of the overall processing and the accuracy of the alignment are improved.

[0048] The sample in the centrifugal sealed test tube is centrifuged by a centrifugal motor and a centrifugal clamping frame, and the sample after centrifugal separation is independently detected and processed by a matching electric push rod, a matching electric push rod, a pumping treatment tube, a pumping treatment tube, a positioning electric sliding rail, an insertion electric push rod, an isolation detection box and an illumination detector, so that multiple different detection and processing of the sample in the same equipment are realized, the efficiency and types of detection are improved, the sample is quickly transferred after insertion combination, isolation shielding and sampling, and the sample is quickly processed, supernatant detection, marker detection and multi-position simultaneous processing are quickly performed, so that in the detection of the culture of biological fermentation, the sample is not directly exposed to the environment due to the non-isolation of the sampling environment, the stability of the sample is affected, the sample is directly and quickly processed by the equipment, the storage time of the sample is reduced, the sample is not changed due to long storage time, the efficiency of actual detection is effectively improved, and the stability of the detection result is improved. The accuracy of the detection data is guaranteed.

[0049] 2. The setting has a folding assembly, which is fixed by a magnetic combination plate, a bearing electric push rod and a universal joint, and the horizontal degree is corrected to ensure the accuracy of the combination connection, the vacuum sampling box is adjusted according to the position and amount of sampling, the sampling position and direction of the clamping connection sleeve are changed by a downward electric motor, a downward lead screw, a downward operation frame and a turnover motor, the simultaneous sampling of different positions in the tank body is realized, the sampling efficiency is improved, the centrifugal sealed test tube is pushed by a taking and placing electric push rod, a taking and placing operation box, a pushing material electric sliding rail and a pushing material operation plate, the detection components are synchronized with sampling, the overall processing speed is improved, and the synchronous processing of different positions is realized through the linkage of multiple components. When the sampling components are fed, the detection components are fed and the sampling position is adjusted, automatic operation can be quickly performed without manual operation of the workers, the influence of the external environment on sampling detection is reduced, the processing speed is improved, the influence of the environment is reduced, the detection efficiency is guaranteed, and the influencing factors are reduced.

[0050] In summary, through the cooperation of the taking and placing double measuring assembly and the folding assembly, the stable environment reduces the impact of the change of the environment before and after sampling on the sample, and through the cooperation of the isolated sampling and the immediate detection after sampling, the sample is quickly analyzed and processed, the influence of the external environment and long waiting time is reduced, the detection accuracy and speed are improved, and manual sampling of the workers is not required, the operation difficulty and labor intensity are reduced. BRIEF DESCRIPTION OF DRAWINGS

[0051] The accompanying drawings are included to provide a further understanding of the application, and are incorporated in and constitute a part of this specification, illustrate embodiments of the application, and together with the description serve to explain the principles of the application. The drawings are provided for merely for illustrative purposes and are not intended to limit the scope of the present application.

[0052] In the drawings:

[0053] Figure 1 is a schematic diagram of the three-dimensional structure of the present application;

[0054] Figure 2 is a schematic diagram of the structure of the pick-and-place double measuring assembly of the present application;

[0055] Figure 3 is a schematic diagram of the mounting structure of the centrifugal engagement frame of the present application;

[0056] Figure 4 is a schematic diagram of the mounting structure of the lifting electric push rod of the present application;

[0057] Figure 5 is a schematic diagram of the mounting structure of the isolation detection box of the present application;

[0058] Figure 6 is a schematic diagram of the mounting structure of the liquid injection operation box of the present application;

[0059] Figure 7 is a schematic diagram of the Figure 6 A area structure of the present application;

[0060] Figure 8 is a schematic diagram of the B area structure of the present application; Figure 6

[0061] Figure 9 is a schematic diagram of the structure of the retractable assembly of the present application;

[0062] Figure 10 is a schematic diagram of the mounting structure of the pick-and-place electric slide rail of the present application;

[0063] Figure 11 is a schematic diagram of the mounting structure of the pick-and-place operation box of the present application;

[0064] Figure label: 1, integrated constant temperature box;

[0065] ​2. Take and place dual-measurement components; 201. Centrifugal motor; 202. Centrifugal clamping frame; 203. Pressing electric push rod; 204. Retracting and retracting electric motor; 205. Retracting and releasing sealing cover; 206. Correcting electric push rod; 207. Correcting operation frame; 208. Lifting electric push rod; 209. Switching motor; 210. Switching operation frame; 211. Swinging motor; 212. Swinging operation frame; 213. Transposition motor; 214. Transposition integration frame; 215. Linkage motor; 216. Transposition Empty block; 217, clamping electric slide; 218, clamping operation block; 219, alignment electric slide; 220, insertion operation frame; 221, insertion electric push rod; 222, isolation detection box; 223, light detector; 224, fitting electric push rod; 225, fitting operation frame; 226, matching electric push rod; 227, matching operation block; 228, extraction and release treatment tube; 229, liquid extraction pump; 230, liquid injection operation box; 231, snap-fit ​​connection sleeve; 232, vacuum sampling box;

[0066] 3. Retractable assembly; 301. Plug-in electric push rod; 302. Processing plug-in box; 303. Discharge and take-out slide rail; 304. Combined electromagnet; 305. Discharge and take-out push rod; 306. Retrieval operation box; 307. Pushing electric slide rail; 308. Pushing operation panel; 309. Load-bearing electric push rod; 310. Universal joint; 311. Magnetic combination plate; 312. Discharge motor lowering; 313. Screw rod lowering; 314. Operating frame lowering; 315. Flip motor; 316. In and out processing tube; 317. Solenoid valve; 318. Push-out operation block; 319. Extrusion electric push rod; 320. Connecting metal ring; 321. Liquid storage fixed box. DETAILED DESCRIPTION

[0067] The preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0068] Example: Figures 1-11 As shown, the present invention provides a technical solution, a detection device for culture of biological fermentation, comprising an integrated constant temperature box 1, a pick-and-place dual-detection component 2 is provided at the side end of the integrated constant temperature box 1;

[0069] The pick-and-place double measuring assembly 2 comprises a centrifugal motor 201, a centrifugal clamping frame 202, a pressing electric push rod 203, a retracting motor 204, a retracting sealing cover 205, a correction electric push rod 206, a correction operation frame 207, a lifting electric push rod 208, a switching motor 209, a switching operation frame 210, a swinging motor 211, a swinging operation frame 212, a transposition motor 213, a transposition integrated frame 214, a linkage motor 215, a transposition hollow block 216, a clamping electric slide rail 217, a clamping operation block 218, a positioning electric slide rail 219, an insertion operation frame 220, an insertion electric push rod 221, an isolation detection box 222, an illumination detector 223, a pasting electric push rod 224, a pasting operation frame 225, a matching electric push rod 226, a matching operation block 227, a pumping treatment tube 228, a liquid pumping pump 229, a liquid injection operation box 230, a clamping connecting sleeve 231, and a vacuum sampling box 232.

[0070] The centrifugal motor 201 is installed at one side of the bottom end of the integrated incubator 1 through a motor base, the output shaft of the centrifugal motor 201 is connected with the centrifugal clamping frame 202, the centrifugal clamping frame 202 is rotatably installed on the inner side of the integrated incubator 1, and the bottom end of the retracting sealing cover 205 is attached to the top end of the centrifugal clamping frame 202, so as to realize stable centrifugal support and centrifugal closing treatment and ensure the stability of centrifugal separation.

[0071] The pressing electric push rod 203 is symmetrically connected at the position close to the centrifugal clamping frame 202 at the bottom end of the integrated incubator 1.

[0072] The top end of the pressing electric push rod 203 is installed with the retracting motor 204 through a motor base, and the output shaft of the retracting motor 204 is connected with the retracting sealing cover 205.

[0073] The correction electric push rod 206 is symmetrically connected at the middle part of the bottom end of the integrated incubator 1, and the top end of each correction electric push rod 206 is connected with the correction operation frame 207.

[0074] The lifting electric push rod 208 is symmetrically installed at the top end of the correction operation frame 207, and the top end of each lifting electric push rod 208 is installed with the switching motor 209 through a motor base.

[0075] The switching operation frame 210 is connected with the output shaft of the switching motor 209, and one end of the switching operation frame 210 is installed with the swinging motor 211 through a motor base.

[0076] The swinging operation frame 212 is connected with the output shaft of the swinging motor 211, the swinging operation frame 212 is rotatably sleeved with the switching operation frame 210, realizes swinging switching and rotating linkage treatment, and one end of the inner side of the swinging operation frame 212 is installed with the transposition motor 213 through a motor base.

[0077] The transposition integrated frame 214 is connected with the output shaft of the transposition motor 213, and the linkage motor 215 is installed at the equidistant positions of the side end of the transposition integrated frame 214 through a motor base.

[0078] The linkage motor 215 output shaft is clamped with a transposition hollow block 216, and the transposition hollow block 216 top end is symmetrically installed with a clamping electric slide rail 217;

[0079] The clamping electric slide rail 217 top end is installed with a clamping operation block 218 through a slide rail seat, the transposition hollow block 216 is six, the transposition hollow block 216 is rotationally installed inside the transposition integrated frame 214, the clamping operation block 218 is slidingly installed at the top end of the transposition hollow block 216, realizing clamping transposition and pick-and-place switching processing;

[0080] The integrated constant temperature box 1 bottom end is symmetrically installed with a positioning electric slide rail 219, and the positioning electric slide rail 219 top end is installed with an insertion operation frame 220 through a slide rail seat;

[0081] The insertion operation frame 220 side end is symmetrically clamped with an insertion electric push rod 221, and the insertion electric push rod 221 one end is clamped with an isolation detection box 222, and the isolation detection box 222 bottom end is slidingly attached to the insertion operation frame 220 top end, the isolation detection box 222 is two, realizing stable isolation processing, and ensuring the stability of detection processing;

[0082] The isolation detection box 222 inside is installed with an illumination detector 223, and the isolation detection box 222 bottom end is clamped with a close electric push rod 224;

[0083] The close electric push rod 224 one end is installed with a close operation frame 225, and the close operation frame 225 top end is installed with a matching electric push rod 226;

[0084] The matching electric push rod 226 bottom end is clamped with a matching operation block 227, and the matching operation block 227 one end is clamped with an exhaust treatment pipe 228;

[0085] The close operation frame 225 top end one side is installed with a liquid pumping pump 229 through a motor seat, and the liquid pumping pump 229 one end is connected with the exhaust treatment pipe 228 one end through an adapter, realizing stable liquid pumping processing, and ensuring the supernatant discharge and injection of detection agent;

[0086] The integrated constant temperature box 1 one end is installed with a liquid injection operation box 230, the liquid injection operation box 230 inside is clamped with a clamping connecting sleeve 231, and the clamping connecting sleeve 231 side end is equidistantly clamped with a plurality of vacuum sampling boxes 232;

[0087] In order to realize stable operation of the equipment, the input ends of the centrifugal motor 201, the pressing electric push rod 203, the receiving and releasing motor 204, the correction electric push rod 206, the lifting electric push rod 208, the switching motor 209, the swinging motor 211, the transposition motor 213, the linkage motor 215, the clamping electric slide rail 217, the positioning electric slide rail 219, the insertion electric push rod 221, the illumination detector 223, the close electric push rod 224, the matching electric push rod 226 and the liquid pumping pump 229 are electrically connected with the output end of the external controller;

[0088] The input end of the external controller is electrically connected with the output end of the external power supply.

[0089] The integrated thermostat 1 is provided with a retractable assembly 3 at one side end.

[0090] The retractable assembly 3 comprises an insertion electric push rod 301, a processing insertion box 302, a taking and placing electric slide rail 303, a combined electromagnet 304, a taking and placing electric push rod 305, a taking and placing operation box 306, a pushing material electric slide rail 307, a pushing material operation plate 308, a load-bearing electric push rod 309, a universal joint 310, a magnetic attraction combination plate 311, a lowering electric machine 312, a lowering lead screw 313, a lowering operation frame 314, a turnover electric machine 315, an in-out processing pipe 316, an electromagnetic valve 317, an external pushing operation block 318, an extrusion electric push rod 319, a connecting metal ring 320 and a liquid storage fixing box 321.

[0091] The integrated thermostat 1 is provided with the insertion electric push rod 301 at one side end, and the insertion electric push rod 301 is provided with the processing insertion box 302 at one end.

[0092] The taking and placing electric slide rail 303 is embedded in the inside end of the processing insertion box 302, and the taking and placing electric slide rail 303 is provided with the combined electromagnet 304 at one end through the slide rail seat and the clamping connection sleeve 231.

[0093] The integrated thermostat 1 is provided with the taking and placing electric push rod 305 at the other side end, and the taking and placing electric push rod 305 is provided with the taking and placing operation box 306 at one end.

[0094] The taking and placing operation box 306 is provided with the pushing material electric slide rail 307 at one end, and the pushing material electric slide rail 307 is provided with the pushing material operation plate 308 at one end through the slide rail seat, and the pushing material operation plate 308 is slidingly installed in the inside of the taking and placing operation box 306, and the taking and placing operation box 306 and the processing insertion box 302 are both two, so as to realize the pushing material taking and placing and the material in-out processing.

[0095] The integrated thermostat 1 is provided with a plurality of load-bearing electric push rods 309 at the side end, and the load-bearing electric push rod 309 is provided with the universal joint 310 at one end, and the universal joint 310 is provided with the magnetic attraction combination plate 311 at the bottom end.

[0096] The lowering electric machine 312 is installed at the top end of the liquid injection operation box 230 through the motor seat, and the lowering lead screw 313 is clamped to the output shaft of the lowering electric machine 312.

[0097] The lowering operation frame 314 is connected to the lead screw seat at the side end of the lowering lead screw 313, and the turnover electric machine 315 is installed at one side of the bottom end of the lowering operation frame 314 through the motor seat, and the output shaft of the turnover electric machine 315 is clamped to the clamping connection sleeve 231 at one end, so as to realize the turnover processing and improve the stability of the turnover clamping.

[0098] An inlet and outlet processing tube 316 is symmetrically passed through one end of the vacuum sampling box 232, and an electromagnetic valve 317 is embedded in one end of the inlet and outlet processing tube 316;

[0099] An external push operation block 318 is slidably connected to one end of the inner side of the vacuum sampling box 232, and an extrusion electric push rod 319 is installed on one end of the inner side of the liquid injection operation box 230. One end of the extrusion electric push rod 319 is fitted with one end of the external push operation block 318 to achieve extrusion fitting and liquid discharge processing;

[0100] A connecting metal ring 320 is clamped at one end of the vacuum sampling box 232, and a liquid storage fixed box 321 is installed on the top of the inner side of the integrated constant temperature box 1;

[0101] In order to ensure the stable operation of the equipment, the input ends of the plug-in electric push rod 301, the discharging slide rail 303, the combined electromagnet 304, the discharging push rod 305, the pushing electric slide rail 307, the load-bearing electric push rod 309, the lowering discharge motor 312, the flipping motor 315, the solenoid valve 317 and the extrusion electric push rod 319 are all electrically connected to the output end of the external controller.

[0102] The working principle and usage process of the present invention are as follows: when testing the fermented material in the fermentation tank, the staff will place the integrated constant temperature box 1 at the top position of the fermentation tank, and put the liquid injection operation box 230 on the sampling port position of the fermentation tank. The equipment is connected to the power supply through an external power supply. When placing, the magnetic combination plate 311 is combined with the magnetic attraction of the fermentation tank, and the load-bearing electric push rod 309 drives the integrated constant temperature box 1 to rise and fall. The universal joint 310 is used to correct the angle, so that the integrated constant temperature box 1 can be stably attached to the top of the fermentation tank, and the levelness of the integrated constant temperature box 1 can be adjusted to ensure its stability during subsequent sampling and testing.

[0103] The processing plug-in box 302 is driven into the liquid injection operation box 230 by the plug-in electric push rod 301, the clamping connecting sleeve 231 is rotated along the lower placing operation frame 314 to change position by cooperating with the overturning motor 315, the processing plug-in box 302 is attached to the side end of the clamping connecting sleeve 231, the vacuum sampling box 232 is moved along the processing plug-in box 302 to the position of the clamping connecting sleeve 231 by the taking and placing electric slide rail 303 driving the combined electromagnet 304, the vacuum sampling box 232 is inserted into the inside of the clamping connecting sleeve 231, at this time the combined electromagnet 304 in the clamping connecting sleeve 231 is magnetically attracted to the connecting metal ring 320, the vacuum sampling box 232 is magnetically attracted and fixed, when one vacuum sampling box 232 is placed, the lower placing screw rod 313 is rotated by the lower placing motor 312, the lower placing operation frame 314 is moved downward along the liquid injection operation box 230 by the lower placing screw rod 313, the clamping connecting sleeve 231 is moved and inserted into the inside of the fermentation tank, the vacuum sampling box 232 is gradually moved downward and gradually inserted, when the vacuum sampling box 232 is completely inserted, the clamping connecting sleeve 231 is completely placed into the fermentation tank by the lower placing screw rod 313 driving the lower placing operation frame 314;

[0104] At the same time, the taking and placing operation box 306 is inserted into the integrated constant temperature box 1 by the taking and placing electric push rod 305, the switching operation frame 210, the swinging operation frame 212 and the position changing integrated frame 214 are rotated by cooperating with the switching motor 209 to change position, the taking and placing operation box 306 is rotated to the position of the taking and placing operation box 306, the centrifugal sealed test tube in the taking and placing operation box 306 at the top is moved along the taking and placing operation box 306 to the side end of the clamping operation block 218 at the position of the hollow block 216 by the pushing material electric slide rail 307 driving the pushing material operation plate 308, the centrifugal sealed test tube is clamped and positioned by the clamping operation block 218 cooperating with the clamping electric slide rail 217, the centrifugal sealed test tube is continuously placed by the position changing integrated frame 214 rotating by cooperating with the position changing motor 213, after the placement is completed, the switching operation frame 210, the swinging operation frame 212 and the position changing integrated frame 214 are reset by the switching motor 209;

[0105] When the clamping connecting sleeve 231 and the vacuum sampling box 232 are completely inserted into the sampling position inside the fermentation tank, the inlet and outlet treatment pipe 316 at the top of the vacuum sampling box 232 is opened by the electromagnetic valve 317, at this time the mixture inside the fermentation tank enters into the inside of the vacuum sampling box 232 along the inlet and outlet treatment pipe 316 under the action of pressure, the sampling treatment is realized, after the sampling is completed, the inlet and outlet treatment pipe 316 is closed by the electromagnetic valve 317, at this time the lower placing operation frame 314, the clamping connecting sleeve 231 and the vacuum sampling box 232 are extracted from the fermentation tank by the lower placing screw rod 313 rotating by the lower placing motor 312, the isolated sampling treatment is realized;

[0106] At the same time of sampling, the correction operation frame 207 is raised by the correction electric push rod 206, the switching electric motor 209 and the switching operation frame 210 are raised by the lifting electric push rod 208, so as to raise the centrifugal sealed test tube to the sample taking position, the switching hollow block 216 is rotated along the switching integration frame 214 by the linkage electric motor 215, the centrifugal sealed test tube is placed flat, the clamping connecting sleeve 231 is rotated along the lower operation frame 314 by the turnover electric motor 315, the inlet and outlet processing tube 316 is rotated to be aligned with the inlet end of the centrifugal sealed test tube, the switching operation frame 210 is rotated by the switching electric motor 209, the centrifugal sealed test tube is connected with the inlet and outlet processing tube 316, the outer push operation block 318 is moved along the vacuum sampling box 232 by the extrusion electric push rod 319, the sample is pushed into the centrifugal sealed test tube from the vacuum sampling box 232, and the sample is discharged into the detection component;

[0107] After the sample is discharged, the centrifugal sealed test tube is reset by rotating the switching hollow block 216 along the switching integration frame 214 by the linkage electric motor 215, the switching operation frame 210 is lowered by the correction electric push rod 206 and the lifting electric push rod 208, the clamping operation block 218 is moved to loosen the centrifugal sealed test tube by the clamping electric slide rail 217, the centrifugal sealed test tube is inserted into the inside of the centrifugal clamping frame 202, after the insertion is completed, the switching operation frame 210 and the switching integration frame 214 are moved by the switching electric motor 209, and the centrifugal clamping frame 202 is moved away from the position, at this time, the take-up motor 204 rotates the take-up sealing cover 205 to be sleeved on the top end of the centrifugal clamping frame 202, the take-up motor 204 and the take-up sealing cover 205 are lowered by the pressing electric push rod 203, the centrifugal clamping frame 202 is rotated along the integration incubator 1 by the centrifugal electric motor 201, and the centrifugal sealed test tube is centrifuged by the centrifugal clamping frame 202;

[0108] After centrifugal standing treatment, the sealing cover 205 is lifted and rotated by the winding and unwinding motor 204 and the pressing electric push rod 203 to open the centrifugal clamping frame 202. At this time, the switching operation frame 210 is rotated again by the switching motor 209 to move the transposition integrated frame 214 to the top of the centrifugal clamping frame 202, and the centrifugal sealing test tube is sleeved with the transposition hollow block 216 again by the descending of the correction electric push rod 206 and the lifting electric push rod 208. The clamping operation block 218 is driven by the clamping electric slide rail 217 to move along the transposition hollow block 216, and the centrifugal sealing test tube is clamped and limited by the clamping operation block 218. The centrifugal sealing test tube is lifted by the correction electric push rod 206 and the lifting electric push rod 208. After the centrifugal sealing test tube is lifted, the insertion operation frame 220 is moved along the integrated incubator 1 by the alignment electric slide rail 219, the isolation detection box 222 is moved along the insertion operation frame 220 by the insertion electric push rod 221, and the isolation detection box 222 is placed at the bottom end of the centrifugal sealing test tube. After the placement is completed, the centrifugal sealing test tube is sleeved inside the isolation detection box 222, and the supernatant after centrifugation is detected by the light detector 223. The detection is completed, the fitting operation frame 225 is moved by the fitting electric push rod 224, the matching operation block 227 is lowered by the matching electric push rod 226, the pumping and processing tube 228 is inserted into the centrifugal sealing test tube, and the supernatant is extracted by cooperating with the pumping pump 229 and the pumping and processing tube 228. After the extraction of the supernatant is completed, the switching operation frame 210 is rotated by the switching motor 209, and the isolation detection box 222 is moved to the bottom end of the centrifugal sealing test tube by the alignment electric slide rail 219 and the insertion electric push rod 221. The labeled detection agent is injected into the centrifugal sealing test tube by the pumping pump 229 and the pumping and processing tube 228. The labeled detection agent is mixed with the precipitate of the sample by rotating and swinging the swinging operation frame 212 and the transposition integrated frame 214 driven by the swinging motor 211, and the part to be detected is labeled. The labeled detection is carried out by cooperating with the isolation detection box 222 and the light detector 223, so that the sample is fully and quickly processed.

[0109] Finally, it should be noted that the above description is only a preferred example of the present application and is not intended to limit the present application. Although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or replace some technical features equivalently. Any modification, equivalent replacement, improvement, etc. within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A detection device for culture of biological fermentation, comprising an integrated constant temperature box (1), characterized in that: The side end of the integrated constant temperature box (1) is provided with a pick-and-place dual-measurement component (2); The pick-and-place dual-measurement assembly (2) includes a centrifugal motor (201); A centrifugal motor (201) is mounted on one side of the bottom end of the integrated constant temperature box (1) via a motor seat, and a centrifugal engaging frame (202) is clamped on the output shaft of the centrifugal motor (201); A press-fit electric push rod (203) is symmetrically connected at the bottom end of the integrated constant temperature box (1) near the centrifugal clamping frame (202); The top end of the pressed electric push rod (203) is mounted on a retractable and retractable motor (204) through a motor seat, and the output shaft of the retractable and retractable motor (204) is clamped with a retractable and retractable sealing cover (205); The middle part of the bottom end of the integrated constant temperature box (1) is symmetrically connected to a correction electric push rod (206), and the top ends of the two correction electric push rods (206) are connected to a correction operation frame (207); The top of the correction operation frame (207) is symmetrically mounted with lifting electric push rods (208), and the tops of the two lifting electric push rods (208) are mounted with switching motors (209) via motor seats; The output shaft of the switching motor (209) is clamped with a switching operating frame (210), and a swing motor (211) is mounted on one end of the switching operating frame (210) via a motor seat.

2. A detection device for biological fermentation culture according to claim 1, characterized in that: The output shaft of the swing motor (211) is clamped with a swing operating frame (212), and a transposition motor (213) is mounted on one inner end of the swing operating frame (212) via a motor seat; The output shaft of the transposition motor (213) is clamped with a transposition integration frame (214), and a linkage motor (215) is installed at equal distances through a motor seat at the side end of the transposition integration frame (214); The output shaft of the linkage motor (215) is clamped with a transposition hollow block (216), and a clamping electric slide rail (217) is symmetrically installed on the top of the transposition hollow block (216); A clamping operation block (218) is installed at the top end of the clamping electric slide rail (217) via a slide rail seat; The centrifugal engaging frame (202) is rotatably mounted on the inner side of the integrated constant temperature box (1), and the bottom end of the retractable sealing cover (205) is fitted with the top end of the centrifugal engaging frame (202).

3. A detection device for biological fermentation culture according to claim 2, characterized in that: The swing operating frame (212) and the switching operating frame (210) are rotatably sleeved together. There are six transposition hollow blocks (216), and the transposition hollow blocks (216) are rotatably mounted on the inner side of the transposition integration frame (214).

4. A detection device for biological fermentation culture according to claim 2, characterized in that: The bottom end of the integrated constant temperature box (1) is symmetrically mounted with an alignment electric slide rail (219), and the top end of the alignment electric slide rail (219) is mounted with an insertion operation frame (220) via a slide rail seat; The side end of the insertion operation frame (220) is symmetrically connected to an insertion electric push rod (221), and one end of the insertion electric push rod (221) is connected to an isolation detection box (222); A light detector (223) is installed inside the isolation detection box (222), and a fitting electric push rod (224) is clamped at the bottom end of the isolation detection box (222).

5. A detection device for biological fermentation culture according to claim 4, characterized in that: The clamping operation block (218) is slidably mounted on the top of the transposition hollow block (216), and the bottom end of the isolation detection box (222) is slidably fitted with the top end of the insertion operation frame (220). There are two isolation detection boxes (222).

6. A detection device for biological fermentation culture according to claim 4, characterized in that: A laminating operation frame (225) is installed at one end of the laminating electric push rod (224), and a matching electric push rod (226) is installed at the top of the laminating operation frame (225); The bottom end of the pairing electric push rod (226) is clamped with a pairing operation block (227), and one end of the pairing operation block (227) is clamped with an extraction and discharge processing tube (228); A liquid pump (229) is installed on one side of the top end of the laminating operation frame (225) via a motor seat; A liquid injection operation box (230) is installed at one end of the integrated constant temperature box (1), a snap-fit ​​connection sleeve (231) is snap-fitted on the inner side of the liquid injection operation box (230), and a plurality of vacuum sampling boxes (232) are snap-fitted at equal distances on the side end of the snap-fit ​​connection sleeve (231).

7. A detection device for biological fermentation culture according to claim 6, characterized in that: One end of the liquid extraction pump (229) is connected to one end of the extraction and treatment pipe (228) via an adapter; The input ends of the centrifugal motor (201), the pressing electric push rod (203), the retracting and discharging motor (204), the correction electric push rod (206), the lifting electric push rod (208), the switching motor (209), the swing motor (211), the transposition motor (213), the linkage motor (215), the clamping electric slide rail (217), the alignment electric slide rail (219), the insertion electric push rod (221), the light detector (223), the fitting electric push rod (224), the matching electric push rod (226) and the liquid pump (229) are all electrically connected to the output end of the external controller; The input end of the external controller is electrically connected to the output end of the external power supply.

8. A detection device for biological fermentation culture according to claim 7, characterized in that: The side end of the integrated constant temperature box (1) is provided with a retractable component (3); The retractable assembly (3) comprises an insertable electric push rod (301); An electric push rod (301) is symmetrically mounted on one end of the integrated constant temperature box (1), and a processing plug box (302) is mounted on one end of the electric push rod (301); A discharge slide rail (303) is embedded in one end of the inner side of the processing plug-in box (302), and a combined electromagnet (304) is installed at one end of the discharge slide rail (303) through a slide rail seat and a snap-fit ​​connection sleeve (231); A discharging push rod (305) is symmetrically mounted on the other side of one end of the integrated constant temperature box (1), and a discharging operation box (306) is mounted on one end of the discharging push rod (305); One end of the inner side of the pick-and-place operation box (306) is clamped with a material pushing electric slide rail (307), and one end of the material pushing electric slide rail (307) is installed with a material pushing operation plate (308) through a slide rail seat; A plurality of load-bearing electric push rods (309) are equidistantly mounted on the side ends of the integrated constant temperature box (1); a universal joint (310) is clamped at one end of the load-bearing electric push rod (309); and a magnetic combination plate (311) is mounted at the bottom end of the universal joint (310); A lowering motor (312) is mounted on the top of the liquid injection operation box (230) via a motor seat, and a lowering screw rod (313) is clamped on the output shaft of the lowering motor (312).

9. A detection device for biological fermentation culture according to claim 8, characterized in that: The side end of the lowering screw rod (313) is connected to a lowering operating frame (314) via a screw rod seat, and a turning motor (315) is installed on one side of the bottom end of the lowering operating frame (314) via a motor seat; An inlet and outlet processing tube (316) is symmetrically passed through one end of the vacuum sampling box (232), and a solenoid valve (317) is embedded in one end of the inlet and outlet processing tube (316); An external push operation block (318) is slidably connected to one end of the inner side of the vacuum sampling box (232), and an extrusion electric push rod (319) is installed at one end of the inner side of the liquid injection operation box (230); One end of the vacuum sampling box (232) is clamped with a connecting metal ring (320), and the top inner side of the integrated constant temperature box (1) is equipped with a liquid storage fixed box (321); The pushing operation plate (308) is slidably mounted on the inner side of the pick-and-place operation box (306), and there are two of each of the pick-and-place operation box (306) and the processing plug-in box (302).

10. A detection device for biological fermentation culture according to claim 9, characterized in that: The output shaft of the flip motor (315) is engaged with one end of the engaging connecting sleeve (231), and one end of the extrusion electric push rod (319) is in contact with one end of the external push operation block (318); The input ends of the plug-in electric push rod (301), the discharging slide rail (303), the combined electromagnet (304), the discharging push rod (305), the material pushing electric slide rail (307), the load-bearing electric push rod (309), the discharging motor (312), the flip motor (315), the solenoid valve (317) and the extrusion electric push rod (319) are all electrically connected to the output end of the external controller.

Citation Information

Patent Citations

  • Detection device for optimum temperature of microbial fermentation of preserved ribs

    CN217997188U