Breeding method for improving mutagenesis efficiency of stem tips of woody plants

By using nanocarrier delivery technology to encapsulate chemical mutagens, gene editing tools and plant hormones in the stem apex of woody plants, combined with intelligent environmental control, the problems of low mutagenesis efficiency and insufficient stress resistance in the stem apex of woody plants have been solved, achieving efficient and precise breeding results.

CN120814482APending Publication Date: 2025-10-21TAIAN TIMES HORTICULTURE TECH DEV CO LTD
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Patent Information

Application Number
CN202511217942.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-08-28
Publication Date
2025-10-21

AI Technical Summary

Technical Problem

The existing stem tip mutagenesis technology for woody plants has the following problems: low mutagenesis efficiency, random mutation types, high cytotoxicity, difficulty in achieving precise and efficient genetic modification, and insufficient attention to stress resistance in breeding methods, resulting in poor performance of new varieties in complex environments, which limits the promotion and application of mutation technology.

Method used

Nanocarriers are used to encapsulate chemical mutagens, gene editing tools and plant hormones, which are precisely delivered to the stem tips of woody plants through microinjection or foliar spraying. Intelligent control systems are combined to regulate environmental factors to achieve synergistic effects of gene editing and hormones, thereby enhancing resistance to diseases and pests, drought, salt and alkali, and other stresses.

Benefits of technology

It significantly improved the efficiency of stem tip mutagenesis and tissue regeneration rate, enhanced the plant's adaptability to multiple adversities such as disease and pest resistance, drought resistance, and salt and alkali resistance, reduced the risk of genetic modification, and improved the accuracy and efficiency of breeding.

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Abstract

The invention belongs to the technical field of plant breeding, and discloses a breeding method for improving the mutagenesis efficiency of stem tips of woody plants. The CRISPR-Cas9 ribonucleoprotein complex and plant hormones 6-benzyladenine, gibberellin and jasmonic acid are co-encapsulated and then accurately delivered to stem tip meristem of woody ornamental plants such as acer truncatum, pteroceltis tatarinowii, crape myrtle and tassel, and the gene mutation obtaining rate of the stem tip meristem is effectively increased; the intelligent environment control system is used for dynamically optimizing illumination, temperature, humidity and oxidative stress, activating a plant endogenous DNA repair pathway and further enhancing homologous recombination efficiency, meanwhile, through collaborative optimization of induction hormone signals and environmental stress, the stress resistance is improved, the leaf color, the plant type and the growth vigor can be accurately regulated and controlled, and the yield of the plant is increased. According to the method, efficient, accurate and stress-resistant linked woody plant stem tip mutation breeding is realized.
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