Production process of oat peptide repairing grain flour mask and mask

Abstract

The invention relates to the technical field of polypeptide skin care masks, in particular to a production process of an oat peptide repairing grain powder mask and the mask thereof, and the production process comprises the following specific steps: S1, taking oat kernel fine powder, rice starch, a white lily bulb extract, a mung bean seed extract, a ginseng leaf / stem extract and a rose extract, and sieving with a 100-120-mesh sieve to obtain basic powder; s2, taking tremella polysaccharide, an oat kernel extract, a ginseng fruit extract, oat peptide and a licorice extract, and activating to obtain an activated component; s3, colloidal oat powder, a snow lotus herb extract, a purslane flower / leaf / stem extract, a cactus extract, aloeicin, the activation component and the basic powder are put into a mixing machine to be mixed, and pretreated mask powder is obtained; s4, air-drying the pretreated mask powder, and sterilizing to obtain a finished product. The problem that the repairing effect is reduced due to the fact that heat-sensitive components such as beta-glucan in tremella polysaccharide and the oat kernel extract are damaged through traditional high-temperature sterilization is solved.

Description

Technical Field

[0001] The present invention relates to the technical field of polypeptide skin care facial masks, in particular to a production process of an oat peptide repairing wheat flour facial mask and the facial mask. Background Art

[0002] Facial masks are skincare products designed specifically for the face. They work by briefly applying or smearing them onto the skin's surface. Common forms include creams, sheets, powders, and gels. Their core principle is to temporarily isolate the external environment through their membrane-like structure, reducing moisture evaporation from the skin while allowing nutrients within the membrane (such as natural plant extracts, active peptides, and moisturizing factors) to more easily penetrate the skin's surface.

[0003] Facial masks offer a wide range of benefits, including cleansing pores, replenishing moisture and nutrients, repairing a damaged skin barrier, and soothing redness and sensitivity. Some masks can also brighten the skin and improve fine lines. Simply follow the instructions and apply for a period of time before washing or removing. It's easy to use.

[0004] Among them, facial mask powder is a powdered facial mask product that forms a soft and gentle film after being mixed with water and applied to the skin. However, the current production process of this type of facial mask still has the following problems:

[0005] 1. During the raw material pretreatment process, the traditional process only uses an 80-100 mesh sieve to filter the raw materials after grinding them. The remaining coarse particles can easily cause a grainy feeling when applying the mask and affect the uniformity of subsequent mixing of ingredients;

[0006] 2. As a small molecule active peptide, oat peptide is susceptible to oxidation when exposed to room temperature or before mixing with other raw materials, resulting in insufficient activity retention, which directly affects the repair effect of the product;

[0007] 3. The existing process mostly adopts single speed stirring throughout the whole process. High-speed stirring easily causes the cereal base to agglomerate, while low-speed stirring cannot achieve full fusion of oat peptides and plant extracts, resulting in uneven distribution of active ingredients in the finished product and large differences in efficacy between batches. Summary of the Invention

[0008] The purpose of the present invention is to solve the technical problems existing in the background technology, and therefore provides a production process and a mask of oat peptide repairing rice flour mask.

[0009] A production process of an oat peptide repairing wheat flour mask, the specific steps are as follows:

[0010] S1. By weight, take 20-35 parts of oat kernel powder, 15-25 parts of rice starch, 0.5-3 parts of white lily bulb extract, 0.5-2.5 parts of mung bean seed extract, 0.3-2 parts of ginseng leaf / stem extract, and 0.5-3 parts of rose extract, and pass them through a 100-120 mesh sieve to obtain a base powder;

[0011] S2, take 0.2-1.5 parts of Tremella polysaccharide, 1-5 parts of oat kernel extract, 0.3-2 parts of ginseng fruit extract, 1-5 parts of oat peptide and 0.5-3 parts of licorice extract, mix them as powder to be activated, add 1-2 times the mass of sterile deionized water of the powder to be activated, place in a constant temperature environment of 40-45 ° C, stir at 50-80 rpm for 30-60 minutes, and obtain an activated component;

[0012] S3, taking 5-12 parts of colloidal oatmeal powder, 0.1-1 parts of snow lotus extract, 0.5-3 parts of purslane flower / leaf / stem extract, 0.3-2 parts of cactus extract, 0.1-1 parts of aloesin, the activated component and the base powder, placing them in a blender, and stirring them alternately clockwise and counterclockwise at 35-40° C. for 40 min-60 min to obtain a pretreated facial mask powder;

[0013] S4. Air-dry the pretreated mask powder at 20-30° C. to a moisture content of ≤1% of the total mass, sterilize in an ultraviolet sterilizer for 20-30 minutes, and then dispense into sterile sealed jars to obtain the finished product.

[0014] As a preferred embodiment of the above technical solution, the white lily bulb extract is prepared by washing the fresh white lily bulb, drying and crushing it to 80-100 mesh, then using 50%-60% ethanol aqueous solution for reflux extraction for 2-3 hours, repeating the extraction twice, after the extraction is completed, combining the two filtrates, centrifuging to obtain the supernatant, and concentrating under reduced pressure to remove ethanol to obtain a concentrated solution, and finally transferring the concentrated solution into a vacuum freeze dryer for drying and then crushing to obtain the concentrate.

[0015] As a preferred embodiment of the above technical solution, the mung bean seed extract is prepared by washing the mung bean seeds, drying and crushing them to 80-100 mesh, then refluxing and extracting them with a 50%-60% ethanol aqueous solution for 2-3 hours, repeating the extraction twice, and after the extraction is completed, combining the two filtrates, centrifuging to obtain the supernatant, and concentrating under reduced pressure to remove ethanol to obtain a concentrated solution, and finally transferring the concentrated solution to a vacuum freeze dryer for drying.

[0016] As a preferred embodiment of the above technical solution, the rose extract is prepared by: selecting dried rose flowers, washing them, and then crushing them into 40-60 mesh sizes. The coarse powder is placed in a percolation cylinder, and a 70%-80% volume fraction of ethanol aqueous solution is used as the percolation solvent. The coarse powder is first soaked for 4-6 hours until it is fully moistened. The percolation is then carried out at a flow rate of 1-2 mL / min. The percolation liquid is collected until the effluent is colorless, concentrated under reduced pressure, and freeze-dried in a vacuum to obtain the extract.

[0017] As a preferred embodiment of the above technical solution, the oat kernel extract is prepared by washing the oat kernel, drying and crushing it to 60-80 mesh size, extracting the residue after reflux defatting with purified water for 2-3 hours, repeating the extraction twice, and after the extraction is completed, combining the two filtrates, concentrating under reduced pressure, and then drying in a vacuum freeze dryer to obtain the extract.

[0018] As a preferred embodiment of the above technical solution, the ginseng fruit extract is prepared by washing the fresh ginseng fruit, drying it, and crushing it into 80-100 meshes. Then, reflux extraction is performed with a 50%-60% ethanol aqueous solution for 2-3 hours, and the extraction is repeated twice. After the extraction is completed, the two filtrates are combined, centrifuged to obtain the supernatant, and concentrated under reduced pressure to remove ethanol to obtain a concentrated solution. Finally, the concentrated solution is transferred to a vacuum freeze dryer for drying.

[0019] As a preferred embodiment of the above technical solution, the licorice extract is prepared by the following method: selecting washed and dried licorice roots and crushing them into 80-100 meshes, then using 50%-60% ethanol aqueous solution for reflux extraction for 2-3 hours, repeating the extraction twice, after the extraction is completed, combining the two filtrates, centrifuging to obtain the supernatant, and concentrating under reduced pressure to remove ethanol to obtain a concentrated solution, and finally transferring the concentrated solution into a vacuum freeze dryer for drying.

[0020] The present invention also provides an oat peptide repairing wheat flour mask, which is prepared by any one of the above production processes.

[0021] The present invention provides a production process for an oat peptide repairing wheat flour mask, which has the following beneficial effects:

[0022] 1. The present invention adopts a step-by-step processing process, using different pretreatment processes for different raw materials. In this way, the cereal base material is not prone to agglomeration during the final stirring and mixing. Low-temperature and high-speed stirring can achieve full fusion of oat peptides, colloidal oatmeal powder and powder, so that the active ingredients in the finished product are evenly distributed, avoiding the problem of liquid plant extracts (such as oat peptides and colloidal oatmeal powder) directly added to the dry powder system causing local deliquescence and forming difficult-to-disperse clots.

[0023] 2. The present invention performs an activation process on oat peptides. As a small molecule active peptide, oat peptides are easily affected by oxidation when exposed at room temperature or before being mixed with other raw materials, resulting in a low activity retention rate. Since the time consumption of the air-drying process is greatly reduced after the activation process, the problem of the unactivated oat peptides being directly stirred and mixed with other raw materials for a long time, which is affected by oxidation and causes a reduction in the repair effect, is avoided.

[0024] 3. The present invention adopts ultraviolet sterilization, which avoids the problem of traditional high-temperature sterilization destroying the heat-sensitive components in Tremella polysaccharide and oat kernel extract, resulting in reduced repair effect. DETAILED DESCRIPTION

[0025] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention.

[0026] Example 1

[0027] A production process of an oat peptide repairing wheat flour mask, the specific steps are as follows:

[0028] S1. By weight, take 20 parts of oat kernel powder, 15 parts of rice starch, 0.5 parts of white lily bulb extract, 0.5 parts of mung bean seed extract, 0.3 parts of ginseng leaf / stem extract and 0.5 parts of rose extract and pass them through a 100-120 mesh sieve to obtain base powder;

[0029] S2. Mix 0.2 parts of Tremella polysaccharide, 1 part of oat kernel extract, 0.3 parts of ginseng fruit extract, 1 part of oat peptide and 0.5 parts of licorice extract as a powder to be activated, add 1.5 times the mass of sterile deionized water of the powder to be activated, place in a constant temperature environment of 40-45°C, and stir at 50 rpm for 30 minutes to obtain an activated component;

[0030] In the specific implementation, Tremella polysaccharide, oat kernel extract, ginseng fruit extract, oat peptide and licorice extract are first mixed as the powder to be activated, and then sterile deionized water is added and stirred at a constant temperature for activation, so that the powder to be activated and the sterile deionized water are evenly mixed to form a paste without obvious particles.

[0031] S3, take 5 parts of colloidal oatmeal powder, 0.1 parts of snow lotus extract, 0.5 parts of purslane flower / leaf / stem extract, 0.3 parts of cactus extract, 0.1 parts of aloesin, the activated component and the base powder, place them in a blender, and stir alternately clockwise and counterclockwise at 35-40° C. for 40 minutes to obtain a pre-treated mask powder;

[0032] S4. Air-dry the pretreated facial mask at 20-30° C. to a moisture content of ≤1% of the total mass, sterilize it in an ultraviolet sterilizer for 20 min, and then package it into sterile sealed cans to obtain the finished product.

[0033] It should be noted that since the particle size and density of each raw material component are different, and facial mask products have extremely high requirements for the uniform dispersion of each raw material component, this means that a wet mixing method must be adopted. The traditional wet mixing method mixes all the solid powder raw materials, adds water and stirs evenly, and then dries. This results in a large amount of water added and a long drying time. The oat peptide in the raw material is a small molecule active peptide, and it is severely oxidized after long-term processing, resulting in a low activity retention rate.

[0034] In S2 of the present invention, oat peptides and other raw material components that are easily inactivated by oxidation are separately activated by adding water. The obtained paste-like activated component can be fully mixed and evenly dispersed with solid powders such as the base powder and colloidal oatmeal powder in S1 and S3 in a short time in the subsequent process. Since the amount of deionized water used in the paste-like activated component is relatively small, the time consumption of the subsequent air-drying process is greatly reduced. As a small molecule active peptide, oat peptide is easily affected by oxidation when exposed to room temperature or before being mixed with other raw materials, resulting in a low activity retention rate. The time consumption of the air-drying process is greatly reduced, thereby avoiding the problem that the oat peptide that has not been activated is directly stirred and mixed with other raw materials for a long time, which is affected by oxidation and causes a reduced repair effect.

[0035] Furthermore, the white lily bulb extract is prepared by washing the fresh white lily bulb, drying it, and crushing it into 80-100 meshes, then using a 50%-60% ethanol aqueous solution for reflux extraction for 2-3 hours, repeating the extraction twice, after the extraction is completed, combining the two filtrates, centrifuging to obtain the supernatant, and concentrating under reduced pressure to remove ethanol to obtain a concentrated solution, and finally transferring the concentrated solution into a vacuum freeze dryer for drying and then crushing it to obtain the concentrate.

[0036] Furthermore, the mung bean seed extract is prepared by washing the mung bean seeds, drying them, and crushing them to 80-100 mesh size. The extract is then extracted with a 50%-60% ethanol aqueous solution under reflux for 2-3 hours, and the extraction is repeated twice. After the extraction is completed, the two filtrates are combined, the supernatant is obtained by centrifugation, and the ethanol is removed by concentration under reduced pressure to obtain a concentrated solution. Finally, the concentrated solution is transferred to a vacuum freeze dryer for drying.

[0037] Furthermore, the rose extract is prepared by: selecting dried rose flowers, washing them, and then crushing them into 40-60 mesh sizes; loading the coarse powder into a percolation cylinder; using a 70%-80% ethanol aqueous solution as a percolation solvent; soaking the coarse powder for 4-6 hours until it is fully moistened; then percolating at a flow rate of 1-2 mL / min; collecting the percolation liquid until the effluent is colorless; concentrating under reduced pressure; and freeze-drying the extract in a vacuum.

[0038] Furthermore, the oat kernel extract is prepared by washing the oat kernel, drying and crushing it into 60-80 mesh, extracting the residue after reflux defatting with purified water for 2-3 hours, repeating the extraction twice, and after the extraction is completed, combining the two filtrates, concentrating under reduced pressure, and then drying in a vacuum freeze dryer to obtain the extract.

[0039] Furthermore, the ginseng fruit extract is prepared by washing the fresh ginseng fruit, drying and crushing it to 80-100 mesh, then refluxing and extracting it with a 50%-60% ethanol aqueous solution for 2-3 hours, repeating the extraction twice, and after the extraction is completed, combining the two filtrates, centrifuging to obtain the supernatant, and concentrating under reduced pressure to remove ethanol to obtain a concentrated solution, and finally transferring the concentrated solution to a vacuum freeze dryer for drying.

[0040] Furthermore, the licorice extract is prepared by: selecting washed and dried licorice roots and crushing them into 80-100 meshes, then using a 50%-60% ethanol aqueous solution for reflux extraction for 2-3 hours, repeating the extraction twice, after the extraction is completed, combining the two filtrates, centrifuging to obtain the supernatant, and concentrating under reduced pressure to remove ethanol to obtain a concentrated solution, and finally transferring the concentrated solution into a vacuum freeze dryer for drying.

[0041] Example 2

[0042] A production process of an oat peptide repairing wheat flour mask, the specific steps are as follows:

[0043] S1. By mass, 35 parts of oat kernel powder, 25 parts of rice starch, 3 parts of white lily bulb extract, 2.5 parts of mung bean seed extract, 2 parts of ginseng leaf / stem extract and 3 parts of rose extract were sieved through a 100-120 mesh sieve to obtain base powder;

[0044] S2, taking 1.5 parts of Tremella polysaccharide, 5 parts of oat kernel extract, 2 parts of ginseng fruit extract, 5 parts of oat peptide and 3 parts of licorice extract, mixing them as the powder to be activated, adding sterile deionized water twice the mass of the powder to be activated, placing it in a constant temperature environment of 40-45°C, stirring at 80 rpm for 60 minutes to obtain an activated component;

[0045] In the specific implementation, Tremella polysaccharide, oat kernel extract, ginseng fruit extract, oat peptide and licorice extract are first mixed as the powder to be activated, and then sterile deionized water is added and stirred at a constant temperature for activation, so that the powder to be activated and the sterile deionized water are evenly mixed to form a paste without obvious particles.

[0046] S3, taking 12 parts of colloidal oatmeal powder, 1 part of snow lotus extract, 3 parts of purslane flower / leaf / stem extract, 2 parts of cactus extract, 1 part of aloesin, the activated component and the base powder, placing them in a blender, and stirring them alternately clockwise and counterclockwise at 35-40° C. for 60 minutes to obtain a pre-treated facial mask powder;

[0047] S4. Air-dry the pretreated facial mask at 20-30° C. to a moisture content of ≤1% of the total mass, sterilize it in an ultraviolet sterilizer for 20-30 minutes, and then package it into sterile sealed cans to obtain the finished product.

[0048] It should be noted that since the particle size and density of each raw material component are different, and facial mask products have extremely high requirements for the uniform dispersion of each raw material component, this means that a wet mixing method must be adopted. The traditional wet mixing method mixes all the solid powder raw materials, adds water and stirs evenly, and then dries. This results in a large amount of water added and a long drying time. The oat peptide in the raw material is a small molecule active peptide, and it is severely oxidized after long-term processing, resulting in a low activity retention rate.

[0049] In S2 of the present invention, oat peptides and other raw material components that are easily inactivated by oxidation are separately activated by adding water. The obtained paste-like activated component can be fully mixed and evenly dispersed with solid powders such as the base powder and colloidal oatmeal powder in S1 and S3 in a short time in the subsequent process. Since the amount of deionized water used in the paste-like activated component is relatively small, the time consumption of the subsequent air-drying process is greatly reduced. As a small molecule active peptide, oat peptide is easily affected by oxidation when exposed to room temperature or before being mixed with other raw materials, resulting in a low activity retention rate. The time consumption of the air-drying process is greatly reduced, thereby avoiding the problem that the oat peptide that has not been activated is directly stirred and mixed with other raw materials for a long time, which is affected by oxidation and causes a reduced repair effect.

[0050] Furthermore, the white lily bulb extract is prepared by washing the fresh white lily bulb, drying it, and crushing it into 80-100 meshes, then using a 50%-60% ethanol aqueous solution for reflux extraction for 2-3 hours, repeating the extraction twice, after the extraction is completed, combining the two filtrates, centrifuging to obtain the supernatant, and concentrating under reduced pressure to remove ethanol to obtain a concentrated solution, and finally transferring the concentrated solution into a vacuum freeze dryer for drying and then crushing it to obtain the concentrate.

[0051] Furthermore, the mung bean seed extract is prepared by washing the mung bean seeds, drying them, and crushing them to 80-100 mesh size. The extract is then extracted with a 50%-60% ethanol aqueous solution under reflux for 2-3 hours, and the extraction is repeated twice. After the extraction is completed, the two filtrates are combined, the supernatant is obtained by centrifugation, and the ethanol is removed by concentration under reduced pressure to obtain a concentrated solution. Finally, the concentrated solution is transferred to a vacuum freeze dryer for drying.

[0052] Furthermore, the rose extract is prepared by: selecting dried rose flowers, washing them, and then crushing them into 40-60 mesh sizes; loading the coarse powder into a percolation cylinder; using a 70%-80% ethanol aqueous solution as a percolation solvent; soaking the coarse powder for 4-6 hours until it is fully moistened; then percolating at a flow rate of 1-2 mL / min; collecting the percolation liquid until the effluent is colorless; concentrating under reduced pressure; and freeze-drying the extract in a vacuum.

[0053] Furthermore, the oat kernel extract is prepared by washing the oat kernel, drying and crushing it into 60-80 mesh, extracting the residue after reflux defatting with purified water for 2-3 hours, repeating the extraction twice, and after the extraction is completed, combining the two filtrates, concentrating under reduced pressure, and then drying in a vacuum freeze dryer to obtain the extract.

[0054] Furthermore, the ginseng fruit extract is prepared by washing the fresh ginseng fruit, drying and crushing it to 80-100 mesh, then refluxing and extracting it with a 50%-60% ethanol aqueous solution for 2-3 hours, repeating the extraction twice, and after the extraction is completed, combining the two filtrates, centrifuging to obtain the supernatant, and concentrating under reduced pressure to remove ethanol to obtain a concentrated solution, and finally transferring the concentrated solution to a vacuum freeze dryer for drying.

[0055] Furthermore, the licorice extract is prepared by: selecting washed and dried licorice roots and crushing them into 80-100 meshes, then using a 50%-60% ethanol aqueous solution for reflux extraction for 2-3 hours, repeating the extraction twice, after the extraction is completed, combining the two filtrates, centrifuging to obtain the supernatant, and concentrating under reduced pressure to remove ethanol to obtain a concentrated solution, and finally transferring the concentrated solution into a vacuum freeze dryer for drying.

[0056] Example 3

[0057] A production process of an oat peptide repairing wheat flour mask, the specific steps are as follows:

[0058] S1. Take 30 parts of oat kernel powder, 20 parts of rice starch, 2 parts of white lily bulb extract, 1 part of mung bean seed extract, 1 part of ginseng leaf / stem extract and 2 parts of rose extract by mass and pass them through a 100-120 mesh sieve to obtain base powder;

[0059] S2. Mix 1 part of Tremella polysaccharide, 3 parts of oat kernel extract, 1 part of ginseng fruit extract, 2 parts of oat peptides and 2 parts of licorice extract as a powder to be activated, add sterile deionized water of 1 times the mass of the powder to be activated, place in a constant temperature environment of 40-45°C, and stir at 60 rpm for 40 minutes to obtain an activated component;

[0060] In the specific implementation, Tremella polysaccharide, oat kernel extract, ginseng fruit extract, oat peptide and licorice extract are first mixed as the powder to be activated, and then sterile deionized water is added and stirred at a constant temperature for activation, so that the powder to be activated and the sterile deionized water are evenly mixed to form a paste without obvious particles.

[0061] S3, taking 7 parts of colloidal oatmeal powder, 0.5 parts of snow lotus extract, 1 part of purslane flower / leaf / stem extract, 1 part of cactus extract, 0.5 parts of aloesin, the activated component and the base powder, placing them in a blender, and stirring them alternately clockwise and counterclockwise at 35-40° C. for 50 minutes to obtain a pre-treated facial mask powder;

[0062] S4. Air-dry the pretreated facial mask at 20-30° C. to a moisture content of ≤1% of the total mass, sterilize in an ultraviolet sterilizer for 25 min, and then package into sterile sealed jars to obtain the finished product.

[0063] It should be noted that since the particle size and density of each raw material component are different, and facial mask products have extremely high requirements for the uniform dispersion of each raw material component, this means that a wet mixing method must be adopted. The traditional wet mixing method mixes all the solid powder raw materials, adds water and stirs evenly, and then dries. This results in a large amount of water added and a long drying time. The oat peptide in the raw material is a small molecule active peptide, and it is severely oxidized after long-term processing, resulting in a low activity retention rate.

[0064] In S2 of the present invention, oat peptides and other raw material components that are easily inactivated by oxidation are separately activated by adding water. The obtained paste-like activated component can be fully mixed and evenly dispersed with solid powders such as the base powder and colloidal oatmeal powder in S1 and S3 in a short time in the subsequent process. Since the amount of deionized water used in the paste-like activated component is relatively small, the time consumption of the subsequent air-drying process is greatly reduced. As a small molecule active peptide, oat peptide is easily affected by oxidation when exposed to room temperature or before being mixed with other raw materials, resulting in a low activity retention rate. The time consumption of the air-drying process is greatly reduced, thereby avoiding the problem that the oat peptide that has not been activated is directly stirred and mixed with other raw materials for a long time, which is affected by oxidation and causes a reduced repair effect.

[0065] Furthermore, the white lily bulb extract is prepared by washing the fresh white lily bulb, drying it, and crushing it into 80-100 meshes, then using a 50%-60% ethanol aqueous solution for reflux extraction for 2-3 hours, repeating the extraction twice, after the extraction is completed, combining the two filtrates, centrifuging to obtain the supernatant, and concentrating under reduced pressure to remove ethanol to obtain a concentrated solution, and finally transferring the concentrated solution into a vacuum freeze dryer for drying and then crushing it to obtain the concentrate.

[0066] Furthermore, the mung bean seed extract is prepared by washing the mung bean seeds, drying them, and crushing them to 80-100 mesh size. The extract is then extracted with a 50%-60% ethanol aqueous solution under reflux for 2-3 hours, and the extraction is repeated twice. After the extraction is completed, the two filtrates are combined, the supernatant is obtained by centrifugation, and the ethanol is removed by concentration under reduced pressure to obtain a concentrated solution. Finally, the concentrated solution is transferred to a vacuum freeze dryer for drying.

[0067] Furthermore, the rose extract is prepared by: selecting dried rose flowers, washing them, and then crushing them into 40-60 mesh sizes; loading the coarse powder into a percolation cylinder; using a 70%-80% ethanol aqueous solution as a percolation solvent; soaking the coarse powder for 4-6 hours until it is fully moistened; then percolating at a flow rate of 1-2 mL / min; collecting the percolation liquid until the effluent is colorless; concentrating under reduced pressure; and freeze-drying the extract in a vacuum.

[0068] Furthermore, the oat kernel extract is prepared by washing the oat kernel, drying and crushing it into 60-80 mesh, extracting the residue after reflux defatting with purified water for 2-3 hours, repeating the extraction twice, and after the extraction is completed, combining the two filtrates, concentrating under reduced pressure, and then drying in a vacuum freeze dryer to obtain the extract.

[0069] Furthermore, the ginseng fruit extract is prepared by washing the fresh ginseng fruit, drying and crushing it to 80-100 mesh, then refluxing and extracting it with a 50%-60% ethanol aqueous solution for 2-3 hours, repeating the extraction twice, and after the extraction is completed, combining the two filtrates, centrifuging to obtain the supernatant, and concentrating under reduced pressure to remove ethanol to obtain a concentrated solution, and finally transferring the concentrated solution to a vacuum freeze dryer for drying.

[0070] Furthermore, the licorice extract is prepared by: selecting washed and dried licorice roots and crushing them into 80-100 meshes, then using a 50%-60% ethanol aqueous solution for reflux extraction for 2-3 hours, repeating the extraction twice, after the extraction is completed, combining the two filtrates, centrifuging to obtain the supernatant, and concentrating under reduced pressure to remove ethanol to obtain a concentrated solution, and finally transferring the concentrated solution into a vacuum freeze dryer for drying.

[0071] Comparative Example 1

[0072] The only difference between Comparative Example 1 and Example 1 is that the activation process of S2 is not adopted. Instead, the Tremella polysaccharide, oat kernel extract, ginseng fruit extract, oat peptide and licorice extract are directly mixed with other components in the S3 process. The other method steps are the same.

[0073] Comparative Example 2

[0074] The only difference between Comparative Example 2 and Example 1 is that the room temperature ultraviolet sterilization process of S4 is not adopted, but the drying process at 85°C is adopted. The other method steps are the same.

[0075] Comparative Example 3

[0076] In this comparative example 3, the oat peptide repairing wheat flour mask prepared in Example 3 was placed at -8°C to 2°C for 24 hours.

[0077] Comparative Example 4

[0078] In this comparative example 4, the oat peptide repairing wheat flour mask prepared in Example 3 was placed at 40±1° C. for 24 hours.

[0079] Blank control group 1

[0080] Existing medical recombinant collagen patches are stored below 4°C for 24 hours.

[0081] Blank control group 2

[0082] Existing medical recombinant collagen patches are stored at 40±1°C for 24 hours.

[0083] The seven kinds of mask powders provided in the above three embodiments and four comparative examples were respectively formulated into mask liquids (mask powder and water were combined in a weight ratio of 1:99), and nine test groups were formed together with the solutions of the recombinant Chinese medicine collagen applied in the blank control groups 1 and 2. According to the principle of the MTT assay, nine cell suspension groups of the same concentration were prepared using skin fibroblasts. During the test, the nine kinds of mask liquids were reacted with each cell suspension group for 48 hours, and then the subsequent treatment of the MTT assay was performed. The absorbance at 570 nm was measured using an enzyme-linked immunosorbent assay (note that in this test, since comparative examples 1-4 and blank control groups 1 and 2 were all unfavorable experimental groups, before starting the nine parallel experiments, Example 3 was used as a benchmark, and the skin fibroblast concentration in the MTT assay was adjusted so that the OD value detected in Example 3 was above 0.9, and this cell concentration was used as the cell concentration of each cell suspension in the test). The test results are shown in Table 1 below:

[0084]

[0085] It can be seen from the above table that the oat peptide repairing wheat flour mask prepared by the process of the present invention has a stronger repair effect than the existing medical recombinant collagen patch. At the same time, the activation process of S2 can significantly enhance the repair effect of the mask. It is speculated that during the activation process, tremella polysaccharide, oat kernel extract, ginseng fruit extract, licorice extract and oat peptide form a polysaccharide-peptide complex to achieve a combined repair function. However, Comparative Example 1 did not adopt the activation process of S2 and directly mixed. It is speculated that tremella polysaccharide, oat kernel extract, ginseng fruit extract, licorice extract and oat peptide were not activated to form a polysaccharide-peptide complex due to interference from other components, resulting in a decrease in repair function.

[0086] In addition, the existing medical recombinant collagen patch also has certain biological activity, but the medical recombinant collagen patch itself is in a solution state and needs to be stored at low temperature to ensure the activity of the recombinant collagen. In the blank control group 2, after being placed at 40±1°C for 24 hours, the activity was significantly reduced, which also shows that the liquid patch has higher storage requirements than the powder mask of this application.

[0087] On this basis, the mask of Example 2 was tested for elastase inhibition rate, hyaluronidase inhibition rate, and cosmetic repair efficacy. The specific process is as follows:

[0088] Elastase inhibition rate test:

[0089] The facial mask of Example 2 was prepared into a 200 mg / mL solution, and a sivelestat sodium solution was used as a positive control, with a test concentration of 40 μg / mL.

[0090] Test method (test standard: Hunan Pharmaceutical University Testing Technology Co., Ltd. HNYD-SOP-041 "Elastase Inhibition Rate Assay Method"): Add 90 μL of buffer, 30 μL of sample diluent of varying concentrations, and 10 μL of substrate to a 96-well plate, mix well, and incubate at 25°C for 20 minutes. Subsequently, add 20 μL of elastase solution and incubate for 10 minutes. The absorbance is then measured. The effect of the sample on elastase activity is calculated, and the statistically significant P value is calculated. The results are shown in the table below.

[0091]

[0092] Hyaluronidase inhibition rate test:

[0093] The facial mask of Example 2 was prepared into a 200 mg / mL solution, and a sodium cromoglycate solution was used as a positive control, with a test concentration of 20 mg / mL.

[0094] Test method (test standard: Hunan Pharmaceutical University Testing Technology Co., Ltd. HNYD-SOP-042 "Hyaluronidase Inhibition Rate Assay Method"): Mix 200 μL each of sample solution, hyaluronidase solution, and sodium hyaluronate, and incubate at 37°C for 20 minutes. Add 200 μL of CTAB solution. Pipette 200 μL of each reaction solution into a 96-well microtiter plate and measure the absorbance at 600 nm. Calculate the effect of each sample on hyaluronidase activity and calculate the P value for statistical significance.

[0095]

[0096] Cosmetic repair efficacy test:

[0097] Usage: Take 500 mg of the facial mask of Example 2, pour an appropriate amount of purified water or distilled water into a bowl, pour it into the powder mask bowl, and mix it into a viscous state; apply it evenly on the face, then soak the mask cloth, apply it to the face and wait for 15-30 minutes, remove the mask cloth and wash it with clean water, and then perform normal skin care.

[0098] Testing process:

[0099] (1) Register the information of the subjects, complete the understanding and signing of the informed consent form, and the experimental technicians screen the subjects according to the inclusion and exclusion criteria;

[0100] (2) The subjects clean the corresponding test sites and sit still in the laboratory at a temperature of 21±1°C and a humidity of 50±10%RH for 30 minutes;

[0101] (3) Use the corresponding equipment to test the baseline values of the subjects' skin;

[0102] (4) The laboratory technicians instruct the subjects to use the samples according to the usage requirements and application sites of the samples, and provide written test precautions and usage instructions;

[0103] (5) The subjects receive the samples and leave the laboratory;

[0104] (6) The subjects fill out the self-assessment questionnaire;

[0105] (7) On the follow-up visit date, the subjects clean the corresponding test sites and sit still in the laboratory at a temperature of 21±1°C and a humidity of 50±10%RH for 30 minutes;

[0106] (8) Use the corresponding equipment to test the skin data values of the subjects after using the samples;

[0107] (9) The subjects fill out the questionnaire, return the samples, and leave the laboratory.

[0108] The detection indexes and instruments are as follows:

[0109]

[0110] Conduct a difference analysis on the above means. When both sets of data are normally distributed, use the paired T-test to analyze the differences between the two sets of data;

[0111] When both sets of data are not normally distributed, use the relevant rank sum test to analyze the differences between the two sets of data.

[0112] Judgment criteria:

[0113] P>0.05 indicates that there is no statistical difference between the two sets of data, denoted by "n.s."; 0.01<P<0.05 indicates statistical significance, denoted by "*"; 0.001<P<0.01 indicates significant difference, denoted by "**"; P<0.001 indicates extremely significant difference, denoted by "***". [[ID=4�]]

[0114]

[0115] Completion status of the subjects:

[0116] This test selected qualified subjects according to the subject inclusion and exclusion criteria. The subjects voluntarily signed a written informed consent, understood the trial process and volunteered to participate in the trial to ensure that a final effective number of 30 subjects was completed (including 30 cases of sensitive skin (lactic acid stinging ≥ 3 points)).

[0117] Adverse reaction feedback during use:

[0118] Each subject used the test sample as required at the specified time. Based on the grading standards for adverse skin reactions in human trial tests specified in the 2015 edition of the "Technical Specifications for Safety of Cosmetics", a human trial study was conducted on 30 subjects. As shown in the table below, no adverse skin reactions were observed.

[0119]

[0120] After seven days of using the sample, all skin reactions in the observed areas were grade 0, with no adverse reactions occurring. Transepidermal water loss and stratum corneum moisture content were tested on 30 people, with the results shown in the table below.

[0121]

[0122]

[0123] It can be seen that the solution of the present invention can effectively improve the moisture content of the user's skin and is gentle and non-irritating.

[0124] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A production process for oat peptide repairing wheat flour mask, characterized in that: The specific steps are as follows: S1. By weight, take 20-35 parts of oat kernel powder, 15-25 parts of rice starch, 0.5-3 parts of white lily bulb extract, 0.5-2.5 parts of mung bean seed extract, 0.3-2 parts of ginseng leaf / stem extract, and 0.5-3 parts of rose extract, and pass them through a 100-120 mesh sieve to obtain a base powder; S2, take 0.2-1.5 parts of Tremella polysaccharide, 1-5 parts of oat kernel extract, 0.3-2 parts of ginseng fruit extract, 1-5 parts of oat peptide and 0.5-3 parts of licorice extract, mix them as powder to be activated, add 1-2 times the mass of sterile deionized water of the powder to be activated, place in a constant temperature environment of 40-45 ° C, stir at 50-80 rpm for 30-60 minutes, and obtain an activated component; S3, taking 5-12 parts of colloidal oatmeal powder, 0.1-1 parts of snow lotus extract, 0.5-3 parts of purslane flower / leaf / stem extract, 0.3-2 parts of cactus extract, 0.1-1 parts of aloesin, the activated component and the base powder, placing them in a blender, and stirring them alternately clockwise and counterclockwise at 35-40° C. for 40 min-60 min to obtain a pretreated facial mask powder; S4. Air-dry the pretreated facial mask at 20-30° C. to a moisture content of ≤1% of the total mass, sterilize it in an ultraviolet sterilizer for 20-30 minutes, and then package it into sterile sealed cans to obtain the finished product.

2. The production process of an oat peptide repairing rice flour mask according to claim 1, characterized in that: The specific steps are as follows: S1. Take 30 parts of oat kernel powder, 20 parts of rice starch, 2 parts of white lily bulb extract, 1 part of mung bean seed extract, 1 part of ginseng leaf / stem extract and 2 parts of rose extract by mass and pass them through a 100-120 mesh sieve to obtain base powder; S2. Mix 1 part of Tremella polysaccharide, 3 parts of oat kernel extract, 1 part of ginseng fruit extract, 2 parts of oat peptides and 2 parts of licorice extract as a powder to be activated, add sterile deionized water of 1 times the mass of the powder to be activated, place in a constant temperature environment of 40-45°C, and stir at 60 rpm for 40 minutes to obtain an activated component; S3, taking 7 parts of colloidal oatmeal powder, 0.5 parts of snow lotus extract, 1 part of purslane flower / leaf / stem extract, 1 part of cactus extract, 0.5 parts of aloesin, the activated component and the base powder, placing them in a blender, and stirring them alternately clockwise and counterclockwise at 35-40° C. for 50 minutes to obtain a pre-treated facial mask powder; S4. Air-dry the pretreated facial mask at 20-30° C. to a moisture content of ≤1% of the total mass, sterilize in an ultraviolet sterilizer for 25 min, and then package into sterile sealed jars to obtain the finished product.

3. The production process of an oat peptide repairing rice flour mask according to claim 2, characterized in that: The white lily bulb extract is prepared by washing fresh white lily bulbs, drying and crushing them into 80-100 mesh sizes, then using a 50%-60% ethanol aqueous solution for reflux extraction for 2-3 hours, repeating the extraction twice, combining the two filtrates after the extraction is completed, centrifuging to obtain the supernatant, and concentrating under reduced pressure to remove ethanol to obtain a concentrated solution, and finally transferring the concentrated solution into a vacuum freeze dryer for drying and then crushing to obtain the extract.

4. The production process of an oat peptide repairing rice flour mask according to claim 2, characterized in that: The mung bean seed extract is prepared by washing the mung bean seeds, drying them, and crushing them into 80-100 mesh sizes. The mung bean seeds are then subjected to reflux extraction with a 50%-60% ethanol aqueous solution for 2-3 hours, and the extraction is repeated twice. After the extraction is completed, the two filtrates are combined, centrifuged to obtain the supernatant, and concentrated under reduced pressure to remove the ethanol to obtain a concentrated solution. Finally, the concentrated solution is transferred to a vacuum freeze dryer for drying.

5. The production process of an oat peptide repairing rice flour mask according to claim 2, characterized in that: The rose extract is prepared by washing and crushing dried rose flowers into 40-60 mesh sizes, placing the coarse powder into a percolation cylinder, using a 70%-80% ethanol aqueous solution as a percolation solvent, soaking the coarse powder for 4-6 hours until it is fully moistened, then percolating at a flow rate of 1-2 mL / min, collecting the percolation liquid until the effluent becomes colorless, concentrating under reduced pressure, and freeze-drying the extract in a vacuum.

6. The production process of an oat peptide repairing rice flour mask according to claim 2, characterized in that: The oat kernel extract is prepared by washing the oat kernel, drying and crushing the oat kernel to 60-80 mesh size, extracting the residue after reflux defatting with purified water for 2-3 hours, repeating the extraction twice, and after the extraction is completed, combining the two filtrates, concentrating under reduced pressure, and then drying in a vacuum freeze dryer to obtain the extract.

7. The production process of an oat peptide repairing rice flour mask according to claim 2, characterized in that: The ginseng fruit extract is prepared by washing fresh ginseng fruit, drying it, and crushing it into 80-100 mesh sizes. The ginseng fruit is then extracted with a 50%-60% ethanol aqueous solution under reflux for 2-3 hours, and the extraction is repeated twice. After the extraction is completed, the two filtrates are combined, centrifuged to obtain the supernatant, and concentrated under reduced pressure to remove the ethanol to obtain a concentrated solution. Finally, the concentrated solution is transferred to a vacuum freeze dryer for drying.

8. The production process of an oat peptide repairing rice flour mask according to claim 2, characterized in that: The preparation method of the licorice extract is as follows: selecting cleaned and dried licorice roots, crushing them into 80-100 meshes, and then using a 50%-60% ethanol aqueous solution to reflux extract for 2-3 hours, repeating the extraction twice, after the extraction is completed, combining the two filtrates, centrifuging to obtain the supernatant, and concentrating under reduced pressure to remove ethanol to obtain a concentrated solution, and finally transferring the concentrated solution to a vacuum freeze dryer for drying.

9. An oat peptide repairing wheat flour mask, characterized in that: The oat peptide repairing wheat flour mask is prepared by any one of the production processes of claims 1-8.

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