Toadstool GZEF01 and application thereof

Abstract

The invention discloses a Morchella elata strain GZEF01 which is preserved in the China Center for Type Culture Collection, and the preservation number of the strain is CCTCC (China Center for Type Culture Collection) NO: M2025943. The invention further discloses application of the strain in preparation of health care products, beverages and the like. The invention also discloses a molecular marker for identifying the strain. The ascocarp of the strain GZEF01 is light brown, the mushroom shape is slender, and the commodity value is high; secondly, the preparation period of the strain is short and is about 29 days, and the production efficiency of the strain is high; the cultivation period of the strain from sowing to fruiting and harvesting is only 65-70 days, the cultivation period is short, the labor cost is reduced, marketing before the Spring Festival is facilitated, and higher selling price and economic benefits are obtained; the strain GZEF01 also has the advantages of high sporocarp yield, strong heavy metal cadmium stress resistance and the like.

Description

Technical Field

[0001] The invention belongs to the technical field of edible fungi, and particularly relates to a Morchella edulis strain and also relates to uses of the strain. Background Art

[0002] Morchella elata (Morchella elata) is rich in nutrients, including eight essential amino acids, vitamins, trace elements, and carbohydrates. It also contains polysaccharides, which have immunomodulatory effects. Furthermore, it is delicious and has a unique flavor. As living standards improve, demand for morels, including Morchella elata, is increasing, but current production is struggling to meet demand.

[0003] At present, the main problems in the production of Morchella are: (1) There are few strains. (2) The production cycle of strains is long. At present, the production cycle of Morchella from mother strain to cultivated strain is generally 58 to 68 days. The strain production time is long and the production efficiency of Morchella strain is not high. (3) The cultivation cycle from sowing to fruiting is long. Usually, the price of fresh Morchella mushrooms on the market before the Spring Festival is high, often several times the price after the Spring Festival. Morchella generally starts field cultivation from the end of October to the beginning of November each year. The growth period of existing Morchella strains from sowing to fruiting is 105 to 120 days, and the fruiting time is from early February to the end of March of the following year. It is difficult to catch up with the fruiting before the Spring Festival, and the profit is relatively low. In addition, the long cultivation cycle means facing more climate risks, which affects the yield and quality of Morchella, as well as the economic benefits. (4) Low yield. The average yield of existing Morchella varieties is only 130 to 147 kg per mu. (5) The heavy metal cadmium content is high in many areas of southwest my country. Generally, "with the increase of cadmium ion stress concentration, the growth rate of Morchella mycelium slows down, until it stops growing" (Li Lang et al., Jiangsu Agricultural Sciences, 2024), which limits the development of Morchella in the local area. Screening or cultivating Morchella that are resistant to cadmium stress is an effective way to solve this problem. At present, there are no reports of Morchella strains that are highly resistant to cadmium stress.

[0004] There are abundant wild morel resources in the primeval forests of southwest my country. Through screening and domestication, we can obtain high-yield morel strains with short production time, short cultivation cycle from sowing to fruiting, high yield and resistance to heavy metal cadmium stress. Summary of the Invention

[0005] The present invention aims to provide a high morel strain.

[0006] Another object of the present invention is to provide uses of the above-mentioned Morchella tataricata strain.

[0007] The third object of the present invention is to provide molecular markers for identifying the above-mentioned Morchella strains.

[0008] To achieve the above object, the technical solution of the present invention is as follows:

[0009] A Morchella elata strain GZEF01 is deposited in the China Center for Type Culture Collection, Wuhan University, Wuhan, China, with a deposit number of CCTCC NO: M2025943 and a deposit date of April 29, 2025.

[0010] The present invention also provides the use of the strain GZEF01 in food or health products.

[0011] The present invention also provides a food or health product, which contains the strain GZEF01 or an extract of GZEF01.

[0012] The present invention also provides the use of the strain GZEF01 in beverages.

[0013] The present invention also provides a beverage containing the strain GZEF01 or an extract of GZEF01.

[0014] The present invention also provides the use of the strain GZEF01 in preparing medicines for improving human immunity.

[0015] The present invention also provides a molecular marker for identifying the strain GZEF01. The molecular marker is a DNA molecule fragment. The nucleotide sequence of the DNA molecule fragment is shown in SEQ ID NO: 1.

[0016] Compared with the existing Morchella strains, the advantages and beneficial technical effects of the present invention are as follows: (1) The ascocarps of the strain GZEF01 of the present invention are light brown, slender in shape, and have high commercial value. (2) The strain GZEF01 of the present invention has a short preparation cycle. The preparation cycle of other existing Morchella mother strains to cultivated strains is 58 to 68 days, while the total preparation cycle of the strain GZEF01 of the present invention from the mother strain to the cultivated strain is only 29 days. The strain preparation cycle of the strain GZEF01 of the present invention is significantly shorter than that of other existing Morchella strains, and the strain production efficiency is higher. (3) The cultivation cycle of the strain GZEF01 of the present invention is short. The cultivation cycle from sowing to fruiting and harvesting of other existing Morchella strains is 105 to 120 days, while the cultivation cycle from sowing to fruiting and harvesting of the strain GZEF01 of the present invention is only 65 to 70 days. GZEF01 has a significantly shorter cultivation cycle than other existing Morchella strains. The short cultivation cycle not only reduces labor costs, but also facilitates the listing of mushrooms before the Spring Festival, thereby obtaining higher prices for fresh mushrooms and economic benefits. In addition, the short cultivation cycle also reduces the climate risks that may be encountered during the cultivation process, and correspondingly reduces the impact on the yield and quality of Morchella. (4) The strain GZEF01 of the present invention has a high fruiting body yield. The strain GZEF01 of the present invention begins agricultural cultivation in the cold greenhouse in the south in early November and is harvested in early January of the following year. It can not only be listed for sale before the Spring Festival, but also has an average yield of 712.3 kg / 667 m 2 , which is much higher than the existing other high morel 130~147kg / 667m 2 The yield of GZEF01 is 34.5-57.4g, and the average seed weight of a single mushroom is 43.74g. (5) The strain GZEF01 of the present invention has a strong resistance to heavy metal cadmium stress. Under different cadmium concentration stresses, its solid seed mycelium growth rate and liquid seed mycelium biomass are significantly higher than other high morel varieties, indicating that its resistance to cadmium stress is significantly higher than other main cultivated high morel varieties, which has expanded the planting area of ​​high morel and increased the income of mushroom farmers. (6) The fruiting body of the strain GZEF01 of the present invention has a large cap proportion, a high dry product rehydration rate, and excellent commercial properties. (7) The fruiting body of the strain GZEF01 of the present invention has a high content of nutrients. In its dried mushrooms, the crude polysaccharide content is 7.58g / 100g, the crude protein content is 46.51g / 100g, and the total amino acid content is 35.56g / 100g.

[0017] Biological preservation: The Morchella elata strain GZEF01 of the present invention was collected by the inventors from a wild Morchella elata strain in Bijie, Guizhou Province in April 2017, and was screened and cultivated; the strain has been deposited in the China Center for Type Culture Collection; the deposit address is: Wuhan University, Wuhan, China; the deposit number is CCTCC NO: M2025943, and the deposit date is April 29, 2025. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 .Photo of the fruiting body of Morchella GZEF01 of the present invention.

[0019] Figure 2 .Phylogenetic tree diagram of Morchella GZEF01 of the present invention. DETAILED DESCRIPTION

[0020] The present invention is further described below with reference to specific examples, which however do not constitute any limitation to the present invention.

[0021] Example 1 Collection, separation, identification and breeding process of Morchella truncatula GZEF01 of the present invention:

[0022] (1) Isolation of bacterial strains

[0023] In April 2017, the inventors accidentally discovered a wild Morchella fruiting body in Bijie, Guizhou Province. Mycelium (species) was obtained by isolating the fruiting body tissue. After morphological characteristics, ITS-PCR detection and phylogenetic tree construction, as well as gene fragment size and sequence comparison, it was identified as Morchella elata. The strain after tissue separation was subjected to a cultivation and fruiting test. Then, the strain was isolated from the fruiting body with good flower shape and inoculated on PDA culture medium for culture. The fruiting test was carried out at the experimental base of Guizhou Academy of Agricultural Sciences. After fruiting, the ascospores were collected and several multi-sporic self-pollinating strains were obtained through multi-sporic self-pollination.

[0024] (2) Strain domestication

[0025] The genomic DNA of the multisporic selfing strain obtained in step (1) was extracted according to the instructions of the DNA extraction kit. Two sets of universal primer combinations P8-F / P8-R and P10-F / P10-R (primers synthesized by Sangon Biotech (Shanghai) Co., Ltd.) were used to amplify the mating type genes MAT1-1 and MAT1-2 of the monosporic strain, respectively. The primer sequences are as follows:

[0026] P8-F: 5'-TTACCTTACTGGACTGGTTCGTGAG-3';

[0027] P8-R: 5'-TGGAATGTCTGTGATTGAGGCTGTG-3'.

[0028] P10-F: 5'-GGCCAGAACAGATGCTCGAAGAAGC-3';

[0029] P10-R: 5'-GTGGCAACTCCCAAAGCATGATCAA-3'.

[0030] The PCR reaction system (total volume 20 μL) consisted of 1 μL of 20-50 ng / μL DNA template, 2 μL of 10× Buffer (with Mg2+), 0.5 μL of 2.5 mM dNTPs, 0.2 μL of 5 U / μL DNA polymerase, 0.5 μL of each 0.2 μM primer, and double-distilled water to 20 μL. PCR reaction conditions included initial denaturation at 94°C for 5 min, followed by 30 cycles of 94°C for 30 s, 63°C for 30 s, and 72°C for 3 min, followed by extension at 72°C for 10 min.

[0031] Electrophoresis of the PCR amplified products revealed that single-spore strains with MAT1-1 but no MAT1-2 gene bands were of the MAT1-1 mating type. Conversely, single-spore strains with MAT1-2 but no MAT1-1 gene bands were of the MAT1-2 mating type. After several generations of cultivation and various comparative tests, a strain with stable genetic traits, rapid mycelial growth, high yield, and strong stress resistance was identified and named GZEF01.

[0032] The strain GZEF01 of the present invention has a complete mating type, and compared with strains isolated from traditional tissues, its genetic material is more complete and its strain traits are more stable. After multiple generations of cultivation tests, its excellent agronomic traits remain stable.

[0033] Example 2 Classification and Identification of the Strain GZEF01 of the Present Invention:

[0034] (1) Morphological identification of GZEF01:

[0035] The ascocarp of GZEF01 is brown, with a fruiting body 10-18 cm tall. The cap is 7-13 cm tall and 2-5 cm thick. It is broadly conical, with a blunt apex and a lower edge connected to the stipe. The ascocarp has well-developed longitudinal ridges and sparse transverse ridges. The ridges are covered with short hairs, and the intersection of the transverse and longitudinal ridges forms a pit with a bottom lighter in color than the ridges. The stipe is 3-6 cm tall and 2-4 cm thick. It is ivory white, cylindrical, and hollow. The asci are columnar, each containing eight spores arranged in a single longitudinal arrangement. According to the morphological characteristics of Morchella elata described on pages 39 and 45 of "Taxonomy and Ecology of Morels in Yunnan" (Li Yingxia, Yunnan Agricultural University, 2015), this strain belongs to the species Morchella elata.

[0036] (2) Molecular biological identification of GZEF01

[0037] The new plant genomic DNA extraction kit CW0531 (purchased from Beijing Kangwei Century Co., Ltd.) was used to extract genomic DNA of the GZEF01 strain according to its instructions. PCR amplification was performed using universal primers ITS1 and ITS4 (primers synthesized by Sangon Biotech (Shanghai) Co., Ltd.). The primer sequences are as follows:

[0038] ITS1: 5'-TCCGTAGGTGAACCTGCGG-3';

[0039] ITS4: 5'-TCCTCCGCTTATTGATATGC-3'.

[0040] The PCR reaction system (total volume 20 μL) is: 1 μL of 20-50 ng / μL DNA template, 10× Buffer (with Mg 2+ ) 2 μL, 0.5 μL of 2.5 mM dNTPs, 0.2 μL of 5 U / μL DNA polymerase, 0.5 μL of each 0.2 μM primer, and double-distilled water to 20 μL. ITS-PCR reaction conditions were: initial denaturation at 94°C for 5 minutes; 30 cycles of 94°C for 1 minute, 60°C for 1 minute, and 72°C for 75 seconds; and extension at 72°C for 10 minutes.

[0041] Results A 689 bp DNA fragment (ITS sequence) was obtained by amplification. The amplified product was sequenced by Sangon Biotech (Shanghai) Co., Ltd. The nucleotide sequence of the DNA fragment is shown in SEQ ID NO: 1.

[0042] The ITS sequence of the obtained GZEF01 strain was subjected to BLAST comparison, and the results showed that its ITS sequence had the highest similarity with Morchella edulis, which could reach 99.71%. A phylogenetic tree was constructed. Figure 2 ) It can be seen that the strain GZEF01 of the present invention belongs to the Morchella elata species; and GZEF01 is different from other existing Morchella elata strains and is a new Morchella elata strain.

[0043] Example 3 Cultivation test of strain GZEF01 of the present invention

[0044] (1) Test materials:

[0045] (1) strain GZEF01 of the present invention; (2) Guizhou Morchella No. 5 (Guizhou Soil and Fertilizer Research Institute); (3) Sichuan Morchella No. 2 (introduced from Sichuan Academy of Agricultural Sciences).

[0046] (2) Test methods

[0047] (1) Stock culture: A sterilized and cooled inoculating hook was used to inoculate a 5 mm diameter GZEF01 strain (this strain has been deposited in the China Center for Type Culture Collection, Wuhan University, Wuhan, China, with the deposit number CCTCC NO: M2025943) in the center of a stock culture medium plate, and cultured at 20°C, 60-75% relative humidity, and in the dark for 5 days until the mycelium covered the plate and a large number of sclerotia formed on the plate, thereby obtaining the GZEF01 stock culture. The components of the stock culture medium and its preparation method are as follows: 200 g of potatoes were peeled and cut into 2 cm cubes, placed in 800 mL of RO water and boiled for 20 min, the extract was filtered through 3 layers of gauze, 20 g of glucose, 20 g of agar, 1 g of potassium dihydrogen phosphate, and 1 g of magnesium sulfate were added, mixed, and then the volume was made up to 1000 mL with RO water, sterilized at 121°C for 30 min, and poured into a 9 cm diameter plate.

[0048] (2) Stock culture: Use a sterilized and cooled spawn shovel to scoop the GZEF01 mother spawn obtained in step (1) at a mass ratio of 1:30 and inoculate it onto the stock culture medium for cultivation. Cultivate at 20°C, 60-75% relative humidity, and avoid light for 12 days until the mycelium fills the flask and a large number of sclerotia form in the flask, thereby obtaining the GZEF01 stock culture medium; wherein the stock culture medium composition and preparation method are as follows: 85% wheat, 8% humus, 5% rice husk, 1% gypsum, 1% calcium carbonate, and the water content of the culture medium is 60-65%. Stir well, put into a polypropylene plastic bag (16 cm × 35 cm × 0.005 cm), tie with a polypropylene rope, and sterilize at 121°C for 2 hours.

[0049] (3) Cultivation of cultivars: Using a sterilized and cooled spawn shovel, scoop the GZEF01 stock obtained in step (2) at a mass ratio of 1:30 and inoculate it onto a cultivar culture medium. Cultivate at 20°C, 60-75% relative humidity, and in the dark for 12 days until a large number of sclerotia form in the flask, thereby obtaining the GZEF01 cultivar. The cultivar culture medium comprises the following components by weight: 85% wheat, 8% humus, 5% rice husk, 1% gypsum, and 1% calcium carbonate, with a water content of 60-65%. Stir evenly, place in a polypropylene plastic bag (16 cm × 35 cm × 0.005 cm), tie with a polypropylene rope, and sterilize at 121°C for 2 hours.

[0050] (4) Fence sowing and film covering: Select arable land with a pH of 6-6.5 and fine soil. After clearing weeds, plow the soil. Before sowing, open the furrows. The furrow width is 80-100 cm. A 50 cm walkway is left between the furrows. The soil moisture content is 40-45%. Break the GZEF01 cultivar obtained in step (3) into small particles and evenly spread them on the furrow surface. Sow 500 bags of cultivar per mu of arable land. Immediately after sowing, evenly cover with a thin layer of soil. Cover with black polypropylene film after covering the soil.

[0051] (5) Mycelium cultivation and placement of nutrient bags: After sowing, the temperature in the greenhouse is controlled at 15-20°C, the relative humidity is 60-70%, the soil moisture content is 40-45%, and the light intensity is 50-100 lux. After 5 days, the mycelium will cover the entire bed and form a white "fungus frost". Then, nutrient bags will be placed. Each bag should have 40-60 small holes, with the perforated surface close to the soil on the bed. 1,800 nutrient bags should be evenly placed per mu of land. After the nutrient bags are placed, they should be covered with black polypropylene film. After the nutrient bags have been placed in the field for 40 days, they should be removed simultaneously, along with the black polypropylene film and nutrient bags. The preparation method of the nutrient bags is as follows: 92% wheat, 7% cottonseed hulls, and 1% quicklime are mixed evenly according to the weight percentage ratio, with the water content of 60-65% and stirred evenly. The cultivation bag uses a polypropylene plastic bag (12cm×24cm×0.005cm) specification. The mixed nutrient material is put into the bag, tied with a polypropylene rope, sterilized at 121℃ for 1 hour, and then cooled to room temperature for use.

[0052] (6) Primordial differentiation: After removing the nutrient bag in step (5), spray water to make the soil moisture content reach 45-55%. During the fruiting body growth stage, control the temperature in the greenhouse at 10-20°C, the relative humidity of the air at 75-85%, and the light intensity at 200-300 lux to induce primordium formation. A large number of primordia will be formed 5 days after spraying water.

[0053] (7) Management and harvesting of mushrooms: The temperature in the greenhouse should be controlled at 10-20°C, relative humidity at 70-80%, soil moisture at 40-45%, light intensity at 500-1000 lux, and carbon dioxide concentration at 500-700 ppm. High temperatures should be cooled by spraying, ventilation through side windows, and other measures. It takes about 15-20 days from the formation of the primordium to the maturity of the fruiting body. When the fruiting body no longer grows, the ridges and pits of the cap are clearly defined, and the ascocarp has basically opened, it is mature and should be harvested by technology.

[0054] The observation results showed that the fruiting body of the strain GZEF01 of the present invention (see Figure 1 The ascocarps of GZEF01 are brown, with fruiting bodies 10-18 cm tall and caps 7-13 cm tall. The caps make up a large portion of the fruiting body, accounting for over 70% of the total height, and are therefore highly valued commercially. Furthermore, the weight of individual GZEF01 mushrooms ranges from 34.5 to 57.4 g, with an average seed weight of 43.74 g per mushroom, indicating a high individual weight.

[0055] From the above steps (1) to (3), it can be seen that the preparation time of the mother strain of the strain GZEF01 of the present invention is 5 days, the preparation time of the original strain from the mother strain is 12 days, and the preparation time of the cultivated strain from the original strain is 12 days, that is, the total preparation cycle from the mother strain to the cultivated strain is 29 days; while the preparation cycle from the mother strain to the cultivated strain of other existing Morchella esculenta is 58 to 68 days. Therefore, the preparation time of the GZEF01 strain is much shorter than that of other existing Morchella esculenta, and the production efficiency of the strain is higher.

[0056] From the above steps (4) to (7), it can be seen that the cultivation cycle of the strain GZEF01 of the present invention is short, and the cultivation cycle from sowing to mushroom harvesting is 65 to 70 days, while the cultivation cycle of other existing high morel strains is 105 to 120 days. The short cultivation cycle not only reduces labor costs, but also facilitates early listing of mushrooms, thereby obtaining higher selling prices and economic benefits. According to the rules of the morel industry, as mentioned in "The Current Situation and Development Thoughts of the Morel Industry in Guizhou Province" published by Wang Qin in the journal "Edible Fungi" in 2022: "Currently, the listing of morels is concentrated in late January to mid-March, and the price of fresh products has fluctuated greatly from the initial 260 yuan / kg to 110 yuan / kg." This indicates that the price of morels first released in January is much higher than that of morels released in large quantities in February and March. As large quantities of fresh morels are released, the price drops significantly. The price of morels initially released is 2 to 3 times the price of morels released in large quantities in February and March. Therefore, the cultivation cycle of the Morchella edulis strain GZEF01 of the present invention, from sowing to fruiting and harvesting, is short, making it more conducive to early fruiting, increasing the price of fresh mushrooms and increasing economic value. Furthermore, the short cultivation cycle reduces the climate risks that may be encountered during the cultivation process, correspondingly reducing the impact on the yield and quality of Morchella edulis.

[0057] As can be seen from Table 1, the strain GZEF01 of the present invention was cultivated in a cold greenhouse in the south starting from early November and harvested in early January of the following year, which was conducive to the listing of fresh mushrooms before the Spring Festival and a higher selling price of fresh mushrooms. In addition, the average fruiting body yield of GZEF01 was 712.3 kg / 667 m 2 , which is much higher than the existing other high morel 130~147kg / 667m 2 of production.

[0058] Table 1 Comparison of the cultivation cycle and yield of GZEF01 of the present invention and other Morchella

[0059] GZEF01 Guizhou Morel No. 5 Sichuan Morel No. 2 Cultivation period (d) 65～70 100～110 110～130 <![CDATA[Yield (kg / 667m 2 )]]> 712.3 147 130

[0060] Example 4 Nutritional component detection test of Morchella oleracea GZEF01 of the present invention

[0061] (1) Test materials: GZEF01 fruiting bodies harvested in Example 3.

[0062] (2) Test methods:

[0063] (1) The harvested fruiting bodies were dried with hot air and the nutritional components in the fruiting bodies were determined.

[0064] (2) The crude polysaccharide content was determined according to the method of NY / T 1676-2023 “Determination of crude polysaccharides in edible fungi”; the protein content was determined according to the method of GB5009.5-2016 “National Food Safety Standard for Determination of Protein in Food”; the total amino acid content was determined according to the method of GB5009.124-2016 “National Food Safety Standard for Determination of Amino Acids in Food”.

[0065] The results showed that the dried mushrooms of the strain GZEF01 of the present invention had a crude polysaccharide content of 7.58 g / 100 g, a crude protein content of 46.51 g / 100 g, and a total amino acid content of 35.56 g / 100 g, indicating that the fruiting bodies of the strain GZEF01 of the present invention have high nutritional value.

[0066] Example 5 Heavy Metal Cadmium Stress Resistance Test of GZEF01 Solid Seed of the Present Invention

[0067] (1) Test materials:

[0068] (1) The strain GZEF01 of the present invention.

[0069] (2) Guizhou Morchella No. 5 (from Guizhou Soil and Fertilizer Research Institute).

[0070] (2) Test methods:

[0071] (1) Mycelial growth under heavy metal cadmium stress in solid culture: Different concentrations of cadmium standard solution were added to PDA culture medium. The final cadmium concentrations of the culture medium were 2 mg / L, 5 mg / L, and 10 mg / L, respectively. PDA culture medium without standard solution was used as blank control (CK). A total of 8 treatments were performed. The pH of all culture media was adjusted to 7. Three replicates were set at different concentrations. The culture blocks were inoculated and cultured in an incubator at 23°C. The mycelial growth in culture medium with different concentrations of heavy metals was recorded.

[0072] (2) Determination of mycelial growth rate: After the mycelial blocks are inoculated into the solid culture medium, when any treatment fills the plate, the culture is stopped, the growth time is recorded, the plate is photographed, and the colony diameter of each treatment is measured using the cross-hatch method to calculate the growth rate.

[0073] The results (see Table 2) show that the mycelial growth rate of the strain GZEF01 under different concentrations of cadmium stress was significantly higher than that of other Morchella edulis. This indicates that its ability to tolerate heavy metal cadmium stress is significantly higher than that of other major cultivated varieties, which expands the cultivation range of Morchella edulis and is beneficial to increasing the income of mushroom farmers.

[0074] Table 2 Comparative test results of the strain GZEF01 solid seed resistance to heavy metal cadmium stress (mycelial growth rate (mm / d))

[0075]

[0076]

[0077] Example 6 Heavy Metal Cadmium Stress Resistance Test of GZEF01 Liquid Seed of the Present Invention

[0078] (1) Test materials:

[0079] (1) The strain GZEF01 of the present invention.

[0080] (2) Guizhou Morchella No. 5 (from Guizhou Soil and Fertilizer Research Institute).

[0081] (2) Test methods:

[0082] (1) Liquid heavy metal cadmium stress: Different concentrations of cadmium standard solution were added to PDB liquid culture medium. The final cadmium concentrations in the culture medium were 2, 5, and 10 mg / L, respectively. The blank control (CK) was set to 0.5. The pH of all culture media was adjusted to 7. Each bottle of culture medium was inoculated with six 5 mm diameter fungus blocks and cultured in a shaker at 23°C, 150 rpm, and in the dark for 5 days. Three replicates were set for each treatment. The mycelial growth in the culture medium with different heavy metal concentrations was recorded.

[0083] (2) Determination of mycelial biomass after heavy metal cadmium stress in liquid culture: Filter the mycelial pellets in the liquid culture medium with a 100-mesh filter and repeatedly rinse the mycelial pellets with RO water. Then, dry the mycelium in an oven at 45°C until constant weight is reached, and weigh the mass of the dry mycelium.

[0084] The results (see Table 3) show that the mycelial biomass of the liquid culture of Morchella oleracea GZEF01 under different cadmium concentrations was significantly higher than that of other Morchella oleracea varieties. This indicates that its tolerance to heavy metal cadmium stress is significantly higher than that of other major cultivated varieties. This expands the cultivation range of Morchella oleracea, which is beneficial for increasing the income of mushroom farmers.

[0085] Table 3 Comparative test results of strain GZEF01 liquid strain resistance to heavy metal cadmium stress (mycelial biomass (g / L))

[0086] Cadmium stress concentration (mg / L) GZEF01(g / L) Guizhou Morel No. 5 (g / L) 0 18.99 6.16 2 12.4 1.66 5 7.46 1.43 10 2.91 0.46

Claims

1. A Morchella elata strain GZEF01, deposited in China Center for Type Culture Collection, with a deposit number of CCTCC NO: M2025943.

2. Use of the strain GZEF01 according to claim 1 in food or health products.

3. A food or health product, characterized in that: The food or health product contains the strain GZEF01 or the extract of GZEF01 according to claim 1.

4. Use of the strain GZEF01 according to claim 1 in beverages.

5. A beverage, characterized in that The beverage contains the strain GZEF01 or the extract of GZEF01 according to claim 1.

6. Use of the strain GZEF01 according to claim 1 in preparing drugs for improving human immunity.

7. A molecular marker for identifying the strain GZEF01 according to claim 1, characterized in that: The molecular marker is a DNA molecule fragment; the nucleotide sequence of the DNA molecule fragment is shown in SEQ ID NO: 1.

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