Sea cucumber peptide enzymolysis device for improving immunity and use method thereof

Abstract

The application relates to the technical field of sea cucumber peptide enzymolysis devices, and discloses a sea cucumber peptide enzymolysis device for improving immunity, which comprises a partitioned temperature control enzymolysis tank, the inner wall of the tank is divided into a top temperature control zone, a middle temperature control zone and a bottom temperature control zone along an axial direction, each temperature control zone is provided with an independent spiral heat exchange coil pipe and a temperature sensor, a center positioning shaft is installed on the axis of the partitioned temperature control enzymolysis tank through a sealing bearing, at least two hollow spiral lifting plates are slidingly sleeved on the center positioning shaft and are hingedly connected with arc-shaped distribution plates, the center positioning shaft is driven to rotate by a planetary gear set of the application, and the lifting plates are lifted by a reciprocating drive shaft, so that spiral circulating flow is formed, compared with a traditional stirring paddle, the mixing efficiency is improved, material stratification and caking are avoided, viscosity data is fed back in real time by an online viscometer, the arc-shaped distribution plates are dynamically adjusted in inclination by the driving of the viscometer, and uniform distribution can be maintained under different viscosity states.

Description

Technical Field

[0001] This invention relates to the field of sea cucumber peptide enzymatic hydrolysis device technology, specifically to a sea cucumber peptide enzymatic hydrolysis device and its usage method for enhancing immunity. Background Technology

[0002] Sea cucumber peptides are products made from sea cucumber body walls or intestines and eggs, through enzymatic hydrolysis to break down large protein molecules into smaller, more bioactive peptides. They are rich in collagen, sea cucumber mucopolysaccharides, amino acids, and trace elements (such as selenium and germanium), exhibiting multiple biological activities including antioxidant, anti-inflammatory, immune-boosting, and cell-repairing properties. Studies have shown that the molecular weight distribution of sea cucumber peptides (especially small peptides below 1000 Daltons) directly affects their absorption efficiency and functional performance. For example, companies like Penglai Shenao have used low-temperature enzymatic hydrolysis technology to control the molecular weight of sea cucumber peptides below 1000 Daltons, significantly improving the bioavailability of nutrients and solving the pain points of "cumbersome soaking and low absorption rate" in traditional sea cucumber consumption.

[0003] Enzymatic hydrolysis is a core step in the production of sea cucumber peptides. Traditional enzymatic hydrolysis devices typically employ a single stirring paddle or a fixed mixing structure, using mechanical stirring to mix the substrate and enzyme solution. However, sea cucumber proteins have large molecular weights and dense structures, and the enzymatic hydrolysis process requires strict control of temperature, pH, and mixing uniformity. For example, literature indicates that the optimal enzymatic hydrolysis conditions for sea cucumber proteins require approximately 52°C for 3 hours, and the use of specific enzymes such as neutral proteases. However, traditional devices generally suffer from the following problems: low mixing efficiency: a single stirring method is insufficient to achieve uniform axial and radial distribution of the material, easily leading to excessively high local concentrations or substrate accumulation (such as at the bottom of the tank), reducing hydrolysis efficiency; insufficient temperature control: traditional devices are mostly designed for overall constant temperature, unable to adapt to the differentiated temperature requirements of different reaction stages (e.g., low temperature for enzyme activation, high temperature for inactivation), easily leading to enzyme inactivation or product degradation; large fluctuations in process parameters: the lack of online monitoring and dynamic adjustment mechanisms, relying on manual intervention, results in unstable product quality between batches.

[0004] Traditional sea cucumber peptide products generally have a degree of hydrolysis below 80%, and the uneven distribution of peptide chain molecular weight limits their application in high-end health products and cosmetics. In contrast, the dynamic distribution delivery unit, through zoned temperature control, gradient dispersion hole design, and a combination of rotation and lifting motion, can precisely regulate material distribution and reaction conditions, providing an innovative solution to these problems. Summary of the Invention

[0005] The purpose of this invention is to provide a sea cucumber peptide enzymatic hydrolysis device to enhance immunity, thereby solving the problems mentioned in the background art.

[0006] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a sea cucumber peptide enzymatic hydrolysis device for improving immunity, comprising:

[0007] The zoned temperature-controlled enzymatic hydrolysis tank has its inner wall divided into a top temperature control zone, a middle temperature control zone, and a bottom temperature control zone along the axial direction. Each temperature control zone is equipped with an independent spiral heat exchange coil and a temperature sensor.

[0008] The central positioning shaft is mounted on the axis of the zoned temperature-controlled enzymatic hydrolysis tank via a sealed bearing.

[0009] At least two hollow spiral lifting plates are slidably sleeved on the central positioning shaft and have an arc-shaped distribution plate hinged to the outer wall;

[0010] The temperature control linkage mechanism is divided into control groups corresponding to the temperature control zones to independently adjust the tilt angle of the corresponding level of the arc-shaped distribution plate;

[0011] A dynamic material conveying channel extends from the central positioning shaft to the arc-shaped distribution plate;

[0012] The composite drive unit synchronously drives the rotation and lifting of the reciprocating drive shaft and the hollow spiral lifting plate through a planetary gear set;

[0013] A multi-parameter sensing system that integrates a temperature sensor, a pH sensor, a dissolved oxygen sensor, a pressure sensor, a weighing sensor, and an online viscometer;

[0014] The rotating interface seat is fixed to the top of the partition temperature-controlled enzymatic hydrolysis tank and is movably connected to the central positioning shaft. Its internal annular cavity is sealed and connected to the dynamic material conveying channel and the external conduit.

[0015] The online viscometer signal is connected to a temperature control linkage mechanism to dynamically adjust the tilt angle of each layer of arc-shaped distribution plate.

[0016] According to the above technical solution, the temperature control linkage mechanism includes:

[0017] A waterproof servo motor embedded in the side wall of the hollow spiral lifting plate;

[0018] The drive gear is located on the output shaft of the waterproof servo motor;

[0019] A driven gear fixed to the hinge shaft of an arc-shaped distribution plate and meshing with the driving gear.

[0020] According to the above technical solution, the composite drive unit includes:

[0021] A titanium alloy connecting plate is fixed to the inner wall of the hollow spiral lifting plate, and the titanium alloy connecting plate moves up and down in the central lifting area of ​​the central positioning shaft.

[0022] A threaded groove running through the titanium alloy connecting plate;

[0023] A reciprocating drive shaft that mates with a threaded groove;

[0024] Limit blocks are installed at both ends of the lifting stroke.

[0025] According to the above technical solution, the planetary gear set includes:

[0026] Fixed bracket;

[0027] A planetary carrier driven by a servo motor;

[0028] The sun gear is fixed to the reciprocating drive shaft;

[0029] Gear ring for fixing the center positioning shaft;

[0030] Hardened planetary gears that mesh with the sun gear and the ring gear.

[0031] According to the above technical solution, in the multi-parameter sensing system:

[0032] Temperature sensors are vertically positioned at the center of each temperature control zone;

[0033] The pH sensor is symmetrically positioned at the axial midpoint of the zoned temperature-controlled enzymatic hydrolysis tank;

[0034] The dissolved oxygen sensor is submerged below the liquid surface;

[0035] The pressure sensor is located in the exhaust pipe;

[0036] The weighing sensor is integrated into the bottom of the zoned temperature-controlled enzymatic hydrolysis tank.

[0037] According to the above technical solution, the rotating interface seat includes:

[0038] The sleeve is connected to the top of the zoned temperature-controlled enzymatic hydrolysis tank via a flange.

[0039] An annular cavity is located on the inner wall of the sleeve and is equipped with a dynamic sealing ring;

[0040] The external conduit radially penetrates the sleeve to connect to the annular cavity.

[0041] According to the above technical solution, the dynamic material conveying channel includes:

[0042] Axial main groove inside the central positioning shaft;

[0043] An elliptical groove connecting the axial main groove;

[0044] The waist-shaped through groove on the side wall of the hollow spiral lifting plate is dynamically sealed with the elliptical groove.

[0045] According to the above technical solution, the zoned temperature-controlled enzymatic hydrolysis tank includes:

[0046] Double-walled insulated tank;

[0047] The top cover integrates coaxially nested auxiliary material tubes and gas tubes;

[0048] The inverted conical bottom cover has a discharge pipe in the center that is coaxial with the central positioning shaft;

[0049] The bottom auxiliary plate is fixed to the bottom of the double-layer insulation tank by circumferentially distributed fixing rods, and the top is coaxially and movably connected to the central positioning shaft and the reciprocating drive shaft;

[0050] Support legs are arranged in a circular array on the side wall of the double-layer insulated tank.

[0051] According to the above technical solution, the surface of the arc-shaped distribution plate is uniformly distributed with gradient dispersion holes, and the hole diameter increases radially from the inside to the outside to form an inner, middle and outer three-ring hole group.

[0052] A method for using a sea cucumber peptide enzymatic hydrolysis device to enhance immunity includes the following steps:

[0053] S1, Parameter Settings

[0054] The control system presets the target temperature values ​​of each temperature control zone, the material ratio parameters of the protease solution and sea cucumber homogenate, and the initial rotation speed during the enzymatic hydrolysis process.

[0055] S2, Co-feeding and Dynamic Mixing

[0056] The protease solution and sea cucumber tissue homogenate are simultaneously fed into the dynamic feed channel, while the composite drive unit is driven to perform the following composite motions:

[0057] The planetary gear set drives the central positioning shaft to rotate continuously.

[0058] The reciprocating drive shaft drives the hollow spiral lifting plate to perform periodic lifting and lowering movements;

[0059] The material is distributed into the three-dimensional space of the zoned temperature-controlled enzymatic hydrolysis tank through the gradient dispersion holes of the arc-shaped distribution plate during the rotation and lifting process.

[0060] S3, Real-time adjustment of distribution status

[0061] Based on the real-time viscosity data of the mixture detected by the online viscometer, the tilt angle of each layer of arc-shaped distribution plate is dynamically adjusted through the temperature control linkage mechanism.

[0062] At the same time, based on the feedback from the temperature sensors in each temperature control zone, the heat exchange intensity of the spiral heat exchange coil in the corresponding zone is independently controlled.

[0063] S4, Closed-loop control of environmental parameters

[0064] When the pH sensor detects that the acidity or alkalinity of the medium deviates from the set range, the acid or alkaline solution tube is selectively opened for neutralization and adjustment.

[0065] When the dissolved oxygen sensor reading is below the critical threshold, inert gas is injected into the liquid through the gas tube.

[0066] S5, Termination of reaction and discharge.

[0067] After the set enzymatic hydrolysis time is reached, the temperature inside the zoned temperature-controlled enzymatic hydrolysis tank is increased to deactivate the enzyme. Then, the discharge pipe of the inverted conical bottom cover is opened to output the enzymatic hydrolysis product.

[0068] Compared with the prior art, the beneficial effects achieved by the present invention are:

[0069] (1) Zoned temperature control and gradient distribution synergistically optimize reaction efficiency: Precise temperature zoning: The top low temperature zone (25-40℃) is used for enzyme activation and prolongs enzyme life; the middle main reaction zone (37-55℃) maintains the best enzyme activity; the bottom high temperature zone (60-85℃) realizes heat inactivation and product purification. Layered temperature control increases the hydrolysis degree of sea cucumber peptide to more than 85%, and the content of immunoactive peptides (such as RGE and RGD sequences) in the product increases significantly; Gradient dispersion wells for precise spraying: The inner circle small wells (1-2mm) are directed to the top low temperature zone, the middle circle medium wells (3-5mm) balance the middle mixing, and the outer circle large wells (5-8mm) accelerate the bottom precipitation, avoiding local overheating or enzyme inactivation, improving mixing uniformity and reducing energy consumption.

[0070] (2) Dynamic distribution conveying unit achieves efficient mixing and distribution: Rotation and lifting compound motion: The planetary gear set drives the central positioning shaft to rotate, combined with the lifting of the reciprocating drive shaft, to form a spiral circulation flow. Compared with the traditional stirring paddle, the mixing efficiency is improved, avoiding material stratification and agglomeration. Temperature control linkage tilt angle adjustment: The online viscometer provides real-time feedback of viscosity data, driving the arc-shaped distribution plate to dynamically adjust the tilt angle, ensuring that uniform distribution can still be maintained under different viscosity conditions, maintaining the viscosity of the mixture at 0.5-2 Pa·s, and preventing enzyme aggregation or excessive hydrolysis.

[0071] (3) Multi-parameter closed-loop control improves process stability: It integrates multiple sensors such as temperature sensor, pH sensor, dissolved oxygen sensor, and links with execution units such as acid and alkali liquid pipe and gas pipe. When the pH deviates, it automatically neutralizes to the set range. When the dissolved oxygen is below the critical value, it injects inert gas (such as nitrogen) to inhibit oxidation and degradation, reduce process parameter fluctuations, and improve product purity.

[0072] (4) Precise control of product quality: The independent temperature control system in each zone controls the axial temperature difference of the enzymatic hydrolysis tank within ±0.5℃. Combined with the closed-loop regulation of pH value and dissolved oxygen, the enzyme activity is kept in the best state. The online viscometer is linked to control the tilt angle of the distribution plate, which effectively avoids local overheating or concentration gradient. Attached Figure Description

[0073] The accompanying drawings are provided to further illustrate the invention and form part of the specification. They are used in conjunction with embodiments of the invention to explain the invention and do not constitute a limitation thereof. In the drawings:

[0074] Figure 1 This is a first perspective view of the present invention;

[0075] Figure 2 This is a second perspective view of the present invention;

[0076] Figure 3 This is a third perspective view of the present invention;

[0077] Figure 4 This is a first partial three-dimensional schematic diagram of the present invention;

[0078] Figure 5 This is a second partial perspective view of the present invention;

[0079] Figure 6 This is a third partial perspective view of the present invention;

[0080] Figure 7 This is a fourth partial perspective view of the present invention;

[0081] Figure 8 This is a fifth partial perspective view of the present invention;

[0082] Figure 9 This is a sixth partial perspective view of the present invention;

[0083] Figure 10 This is a third-dimensional schematic diagram of the seventh part of the present invention;

[0084] Figure 11 This is the eighth partial perspective view of the present invention;

[0085] Figure 12 This is a third-dimensional schematic diagram of the ninth part of the present invention;

[0086] Figure 13 This is the present invention. Figure 8 A magnified view of a portion of point A in the middle;

[0087] Figure 14 This is the present invention. Figure 10 A magnified view of a portion of point B in the middle;

[0088] Figure 15 This is the present invention. Figure 11 A magnified view of a portion of point C in the middle;

[0089] In the diagram: 100-zone-controlled temperature-controlled enzymatic hydrolysis tank, 110-independent spiral heat exchange coil, 120-double-layer insulated tank body, 130-top cover, 131-auxiliary material pipe, 132-gas pipe, 140-inverted conical bottom cover, 141-discharge pipe, 150-bottom auxiliary support plate, 160-fixing rod, 170-support leg, 200-central positioning shaft, 210-sealed bearing, 220-central lifting area, 300-hollow spiral lifting plate, 310-arc-shaped distribution plate, 311-hinge shaft, 312-gradient dispersion hole, 320-temperature control linkage mechanism, 321-waterproof servo motor, 322-drive gear, 323-driven gear, 330-dynamic material conveying channel, 331-axial main groove, 332- Elliptical groove, 333-waisted through groove, 400-composite drive unit, 410-reciprocating drive shaft, 420-titanium alloy connecting plate, 421-threaded groove, 430-limiting block, 500-planetary gear set, 510-fixed bracket, 520-servo motor, 530-planetary carrier, 540-sun gear, 550-gear ring, 560-hardened planetary gear, 600-multi-parameter sensing system, 610-temperature sensor, 620-pH sensor, 630-dissolved oxygen sensor, 640-pressure sensor, 650-weighing sensor, 660-online viscometer, 700-rotary interface seat, 710-annular cavity, 711-dynamic sealing ring, 720-sleeve, 730-external conduit. Detailed Implementation

[0090] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0091] Please see Figure 1-15 The present invention provides a technical solution: a sea cucumber peptide enzymatic hydrolysis device for enhancing immunity, comprising:

[0092] The zoned temperature-controlled enzymatic hydrolysis vessel 100 has its inner wall divided into a top temperature control zone, a middle temperature control zone, and a bottom temperature control zone along the axial direction. Each temperature control zone is equipped with an independent spiral heat exchange coil 110 and a temperature sensor 610.

[0093] The central positioning shaft 200 is mounted on the axis of the partition temperature-controlled enzymatic hydrolysis tank 100 via a sealed bearing 210.

[0094] At least two hollow spiral lifting plates 300 are slidably sleeved on the central positioning shaft 200 and have an arc-shaped distribution plate 310 hinged to the outer wall;

[0095] The temperature control linkage mechanism 320 is equipped with control groups corresponding to the temperature control zone to independently adjust the tilt angle of the corresponding level arc distribution plate 310.

[0096] The dynamic material conveying channel 330 extends from the central positioning shaft 200 to the arc-shaped distribution plate 310.

[0097] The composite drive unit 400 synchronously drives the reciprocating drive shaft 410 and the hollow spiral lifting plate 300 to rotate and lift through the planetary gear set 500.

[0098] The multi-parameter sensing system 600 integrates a temperature sensor 610, a pH sensor 620, a dissolved oxygen sensor 630, a pressure sensor 640, a weighing sensor 650, and an online viscometer 660.

[0099] The rotating interface seat 700 is fixed to the top of the partition temperature-controlled enzymatic hydrolysis tank 100 and movably connected to the central positioning shaft 200. Its internal annular cavity 710 is sealed and connected to the dynamic material conveying channel 330 and the external conduit.

[0100] The online viscometer 660 is connected to the temperature control linkage mechanism 320 to dynamically adjust the tilt angle of each layer of arc-shaped distribution plate 310.

[0101] Specifically, the temperature control linkage mechanism 320 includes:

[0102] Waterproof servo motor 321 embedded in the side wall of hollow spiral lifting plate 300;

[0103] The drive gear 322 is located on the output shaft of the waterproof servo motor 321;

[0104] A driven gear 323 is fixed to the hinge shaft 311 of the arc-shaped distribution plate 310 and meshes with the driving gear 322;

[0105] Specifically, the composite drive unit 400 includes:

[0106] A titanium alloy connecting plate 420 is fixed to the inner wall of the hollow spiral lifting plate 300. The titanium alloy connecting plate 420 moves up and down in the central lifting area 220 of the central positioning shaft 200.

[0107] A threaded groove 421 penetrating the titanium alloy connecting plate 420;

[0108] A reciprocating drive shaft 410 that mates with the threaded groove 421;

[0109] Limit blocks 430 are installed at both ends of the lifting stroke;

[0110] Specifically, the planetary gear set 500 includes:

[0111] Fixed bracket 510;

[0112] The planetary carrier 530 is driven by the servo motor 520;

[0113] The sun gear 540 is fixed to the reciprocating drive shaft 410;

[0114] Gear ring 550 is fixed to the center positioning shaft 200;

[0115] Hardened planetary gear 560 meshes with sun gear 540 and ring gear 550;

[0116] Specifically, in the multi-parameter sensing system 600:

[0117] Temperature sensor 610 is vertically positioned at the center of each temperature control zone;

[0118] The pH sensor 620 is symmetrically positioned at the midpoint of the axial direction of the zoned temperature-controlled enzymatic hydrolysis tank 100;

[0119] The dissolved oxygen sensor 630 is submerged below the liquid surface;

[0120] Pressure sensor 640 is located in the exhaust pipe;

[0121] Weighing sensor 650 is integrated into the bottom of zoned temperature-controlled enzymatic hydrolysis tank 100;

[0122] Specifically, the rotating interface base 700 includes:

[0123] Sleeve 720 is connected to the top of partition temperature-controlled enzymatic hydrolysis tank 100 via flange;

[0124] An annular cavity 710 is located on the inner wall of the sleeve 720 and is equipped with a dynamic sealing ring 711;

[0125] The external conduit 730 radially penetrates the sleeve 720 and connects to the annular cavity 710;

[0126] Specifically, the dynamic material conveying channel 330 includes:

[0127] Axial main groove 331 within the central positioning shaft 200;

[0128] Elliptical groove 332 connecting the axial main groove 331;

[0129] The waist-shaped through groove 333 on the side wall of the hollow spiral lifting plate 300 is dynamically sealed with the elliptical groove 332.

[0130] Specifically, the zoned temperature-controlled enzymatic hydrolysis tank 100 includes:

[0131] Double-walled insulated tank, 120;

[0132] The top cover 130 integrates a coaxially nested auxiliary material tube 131 and a gas tube 132;

[0133] The inverted conical bottom cover 140 has a discharge pipe 141 coaxial with the central positioning shaft 200 in the center;

[0134] The bottom auxiliary plate 150 is fixed to the bottom of the double-layer heat preservation tank 120 by the circumferentially distributed fixing rods 160, and the top is coaxially and movably connected to the central positioning shaft 200 and the reciprocating drive shaft 410.

[0135] Support legs 170 are arranged in a circular array on the side wall of the double-layer insulated tank 120;

[0136] Specifically, the surface of the arc-shaped distribution plate 310 is evenly distributed with gradient dispersion holes 312, and the hole diameter increases radially from the inside to the outside to form an inner, middle and outer three-ring hole group;

[0137] A method for using a sea cucumber peptide enzymatic hydrolysis device to enhance immunity includes the following steps:

[0138] S1, parameter preset

[0139] The following parameters are preset in the control system:

[0140] S11, the independent target temperature values ​​of the top temperature control zone, middle temperature control zone and bottom temperature control zone of the enzymatic hydrolysis tank, wherein the absolute value of the target temperature difference between the top and bottom temperature control zones is ≤3℃;

[0141] S12, the ratio of the concentration of the protease solution to the solid content of the sea cucumber tissue homogenate;

[0142] S13, the initial rotational speed and lifting / lowering frequency of the planetary drive unit;

[0143] S2, Dynamic Cooperative Feeding

[0144] Perform the following operations simultaneously:

[0145] S21, through the dynamic feeding channel 330 inside the central positioning shaft 200, the protease solution and sea cucumber tissue homogenate are continuously fed into the enzymatic hydrolysis tank at a preset ratio;

[0146] S22, start the compound drive unit 400, drive the center positioning shaft 200 to rotate continuously through the planetary gear set 500, and at the same time drive the hollow spiral lifting plate 300 to perform periodic lifting motion through the reciprocating drive shaft 410.

[0147] S3, Real-time adjustment of distribution status

[0148] Dynamic control based on online monitoring data:

[0149] S31, based on the viscosity value of the mixture detected in real time by the online viscometer 660, the tilt angle of each layer of arc-shaped distribution plate 310 is adjusted by the temperature control linkage mechanism 320;

[0150] S32, based on the feedback signals from the temperature sensors 610 of each temperature control zone, independently controls the heat exchange power of the corresponding spiral heat exchange coil 110;

[0151] S4, closed-loop regulation of the reaction environment

[0152] Adjustment is initiated under the following conditions:

[0153] S41, When the pH sensor 620 detects a value that exceeds the set value by ±0.3, open the acid or alkali pipe of the top cover 130 to inject the adjusting solution.

[0154] S42, When the dissolved oxygen sensor 630 detects a value below the critical threshold, inert gas is injected into the liquid through the gas tube 132;

[0155] S5, termination of enzymatic hydrolysis and product output

[0156] Execute in sequence:

[0157] S51, after the preset enzymatic hydrolysis time is reached, raise the temperature inside the enzymatic hydrolysis tank to 80-90℃ and maintain it for 10-20 minutes;

[0158] S52, open the discharge pipe 141 of the inverted conical bottom cover 140 to output the enzymatic hydrolysate, and maintain the bottom auxiliary plate 150 in axial positioning of the material during the discharge process.

[0159] Working Principle: This device achieves efficient enzymatic hydrolysis of sea cucumber peptides through the synergistic action of multiple systems. Its core lies in the precise matching of the enzymatic hydrolysis environment and material distribution through dynamic regulation. Upon startup, the composite drive unit synchronously drives the rotation of the central positioning shaft and the periodic lifting motion of the hollow spiral lifting plate. This causes the articulated arc-shaped distribution plate to form a three-dimensional dynamic distribution network. The protease solution and sea cucumber homogenate are conveyed to the arc-shaped distribution plate through the dynamic conveying channel. The gradient dispersion holes achieve a combined radial diffusion and axial lifting motion, significantly improving the uniformity of material mixing.

[0160] During operation, a multi-parameter sensing system monitors the viscosity, temperature, pH value, and dissolved oxygen parameters of the mixture in real time. Data from the online viscometer is fed back to the temperature control linkage mechanism, driving a waterproof servo motor to adjust the tilt angle of the arc-shaped distribution plate. This dynamically adapts the material distribution to the current viscosity characteristics. The three temperature control zones of the zoned temperature-controlled enzymatic hydrolysis tank achieve precise temperature control through independent spiral heat exchange coils, maintaining temperature fluctuations in each zone within ±0.5℃ to ensure optimal enzyme activity.

[0161] When the pH value deviates from the set threshold, the control system selectively opens the acid or alkali pipe of the top cover for rapid neutralization. When dissolved oxygen is insufficient, the coaxially nested gas pipe injects inert gas into the system to prevent oxidation reactions from affecting product quality. The planetary gear set, through the precise meshing of the sun gear and the gear ring, and with the cooperation of the titanium alloy connecting plate and the threaded groove, converts the rotational torque into lifting kinetic energy, realizing real-time adaptation of motion parameters and material characteristics. After the enzymatic hydrolysis is completed, the system raises the temperature inside the tank to inactivate the enzyme preparation, and outputs high-purity enzymatic hydrolysate through the collinear discharge pipe of the inverted conical bottom cover. The entire process, through the deep integration of structural innovation and intelligent control, improves the yield of immunologically active peptides and increases the concentration of molecular weight distribution.

[0162] The following is a description of the functional structure of each component of this device:

[0163] 100 zoned temperature-controlled enzymatic hydrolysis tank

[0164] Axial layered design: The tank is divided into three temperature control zones along the axial direction: top, middle and bottom. The zone temperature is precisely controlled by independent spiral heat exchange coils 110. The top zone is suitable for maintaining enzyme activity at low temperature, the middle zone is the main reaction zone for enzymatic hydrolysis, and the bottom zone is used for thermal inactivation or accelerated reaction.

[0165] Dynamic thermal response: Temperature sensor 610 monitors the temperature of each layer in real time, and combined with the adjustment of spiral heat exchange coil 110, it ensures that the enzymatic hydrolysis reaction at different stages is carried out in the optimal temperature range (such as the protease activity temperature of 37-50℃).

[0166] Double-walled insulated tank 120: Reduces heat loss and maintains the stability of zoned temperature control.

[0167] The inverted conical bottom cover 140 and the discharge pipe 141 are designed to be on the same line, which facilitates thorough material discharge.

[0168] The top of the zone-controlled temperature enzymatic hydrolysis tank 100 also integrates existing components such as acid pipe, alkali pipe, pure water pipe, and exhaust pipe, which are well-known technologies and will not be described in detail here. The zone-controlled temperature enzymatic hydrolysis tank 100 divides the tank body axially into three independent temperature control zones: top, middle, and bottom. Each zone achieves precise temperature control through a spiral heat exchange coil 110 and a temperature sensor 610. Its technical advantages are as follows:

[0169] Top temperature control area

[0170] Functional positioning: Low temperature pretreatment and enzyme activation zone.

[0171] Temperature range: 25-40℃ (adjust according to the type of enzyme).

[0172] Core function:

[0173] Enzyme activation: Slowly activate the enzyme at low temperature to avoid enzyme inactivation caused by sudden high temperature changes.

[0174] Substrate dispersion: The sea cucumber homogenate and enzyme solution are evenly sprayed onto the upper part of the tank through the gradient dispersion holes 312 of the arc-shaped distribution plate 310 to avoid excessively high local concentrations.

[0175] pH pre-adjustment: The pH of the system is initially adjusted using the acid / alkali solution tube to lay the foundation for subsequent reactions.

[0176] Central temperature control zone

[0177] Functional positioning: main reaction zone.

[0178] Temperature range: 37-55℃ (peak range of protease activity).

[0179] Core function:

[0180] Highly efficient enzymatic hydrolysis: Maintaining the optimal temperature for enzyme activity accelerates the hydrolysis of sea cucumber collagen.

[0181] Dynamic mixing: The composite drive unit 400 drives the hollow spiral lifting plate 300 to rotate and lift, which, together with the gradient dispersion holes of the arc-shaped distribution plate 310, forms a three-dimensional circulating flow to avoid material stratification.

[0182] Viscosity control: The online viscometer 660 provides real-time feedback on viscosity data, and the temperature control linkage mechanism 320 dynamically adjusts the tilt angle of the distribution plate to maintain the viscosity of the mixture at 0.5-2 Pa·s (to prevent enzyme aggregation).

[0183] Bottom temperature control area

[0184] Functional positioning: Thermal inactivation and product optimization zone.

[0185] Temperature range: 60-85℃ (adjusted according to the target product).

[0186] Core function:

[0187] Enzyme inactivation: Inactivating enzymes by raising the temperature, terminating the reaction, and avoiding excessive hydrolysis that damages the peptide chain structure.

[0188] Product purification: High temperature promotes the precipitation of small molecule peptides, and the material is quickly discharged through the discharge pipe 141 of the inverted conical bottom cover 140 to reduce impurity residue.

[0189] Energy-saving design: The waste heat at the bottom is used to preheat the material entering the tank, reducing overall energy consumption.

[0190] Synergistic effect of zoned temperature control

[0191] Temperature gradient optimizes reaction pathway:

[0192] The low-temperature zone at the top prolongs enzyme life, the high-temperature zone in the middle accelerates the reaction, and the high-temperature zone at the bottom completes inactivation, forming a complete process chain of "activation-reaction-termination".

[0193] Compared to traditional single-temperature zone tanks, zoned temperature control can increase the yield of sea cucumber peptides by 15-20%.

[0194] Maximizing space utilization:

[0195] The enzyme solution is sprayed to different heights in the tank through the gradient dispersion holes 312 of the arc-shaped distribution plate 310 to match the reaction requirements of each temperature control zone.

[0196] The dynamic material conveying channel 330 (elliptical groove 332 and waist-shaped through groove 333 are sealed together) ensures that the material is evenly distributed along the axial direction and avoids dead corners.

[0197] Balancing energy consumption and safety:

[0198] The spiral heat exchange coil 110 adopts independent heating / cooling in zones, which reduces heat loss by 30% compared to the traditional jacketed design.

[0199] Pressure sensor 640 is linked to exhaust pipe 108 to prevent the risk of overpressure explosion during steam heating.

[0200] The dynamic distribution conveying unit (central positioning shaft 200 + hollow spiral lifting plate 300 + temperature control linkage mechanism 320) is the core actuator of the sea cucumber peptide enzymatic hydrolysis device of this invention. Through rotation-lifting compound motion, dynamic tilt angle adjustment, and three-dimensional material distribution, it achieves efficient mixing and distribution of substrate and enzyme solution during the enzymatic hydrolysis reaction. The following is a detailed structural analysis:

[0201] Center positioning axis 200

[0202] Structural features:

[0203] The sealed bearing 210 is installed on the central axis of the zoned temperature-controlled enzymatic hydrolysis tank 100 to ensure rotational stability.

[0204] The internally integrated axial vertical connecting groove 341 and eccentric elliptical groove 342 serve as the main body of the dynamic material conveying channel 330.

[0205] Functions and uses:

[0206] Motion reference: Provides a motion reference for the rotation and lifting of the subsequent hollow spiral lifting plate 300 and arc-shaped distribution plate 310.

[0207] Material conveying: The protease solution and sea cucumber homogenate fed by the external feed pump are conveyed to each temperature-controlled zone through the internal channel.

[0208] Hollow spiral lifting plate 300

[0209] Structural features:

[0210] It is slidably sleeved on the central positioning shaft 200, and the outer wall is hinged to the arc-shaped distribution plate 310.

[0211] A titanium alloy connecting plate 420 is fixed on the inner wall, and lifting is achieved by cooperating with the reciprocating drive shaft 410 through the threaded groove 421.

[0212] Functions and uses:

[0213] Dynamic lifting: The composite drive unit 400 drives the reciprocating shaft 410 to rotate through the planetary gear set 500, causing the hollow spiral lifting plate 300 to move up and down along the central axis, which in turn drives the arc-shaped distribution plate 310 to move vertically.

[0214] Rotation synchronization: The planetary gear set 500 simultaneously drives the central positioning shaft 200 to rotate, so that the hollow spiral lifting plate 300 continues to rotate during the lifting process, forming a compound motion trajectory.

[0215] Arc-shaped distribution plate 310

[0216] Structural features:

[0217] The surface is uniformly distributed with gradient dispersion holes 312, and the hole diameter increases radially from the inside to the outside, divided into three groups: inner ring, middle ring and outer ring.

[0218] One end engages with the drive gear 322 driven by the waterproof servo motor 321 via the hinge shaft 311 to achieve tilt angle adjustment.

[0219] Functions and uses:

[0220] Material spraying: Enzyme solution and sea cucumber homogenate are sprayed to different temperature control zones through gradient dispersion holes 312. In this device, during enzymatic hydrolysis, the distribution plate needs to be rotated 180 degrees to position the spraying surface on the lower side of the arc-shaped distribution plate 310. Its main function is to spray the enzyme solution and sea cucumber homogenate conveyed by the dynamic material conveying channel 330 downwards to different temperature control zones of the zoned temperature-controlled enzymatic hydrolysis tank 100. The inner ring small holes are located on the lower side of the arc-shaped distribution plate 310 near the central positioning axis 200, covering the top low-temperature zone (25-40℃). The small hole spray forms a fine mist of material, promoting the initial mixing of sea cucumber homogenate and enzyme solution, and accurately spraying the protease solution and sea cucumber homogenate to the top low-temperature zone, extending enzyme life and avoiding enzyme inactivation caused by high temperature. The middle ring central hole is located on the lower side of the middle section of the arc-shaped distribution plate 310. The material sprayed through the central hole covers the main reaction zone (37-55℃). The material sprayed through the central hole forms a circulating flow with the material at the top / bottom, avoiding excessively high or low local concentrations. At the optimal enzyme activity temperature, the material sprayed through the central hole comes into full contact with the enzyme solution, accelerating the hydrolysis of sea cucumber collagen. The large holes in the outer ring are located on the lower side of the outer edge of the arc-shaped distribution plate 310, covering the high-temperature zone at the bottom (60-85℃). The turbulence formed by the large hole spray enhances the mixing efficiency of the bottom material, reducing precipitation and clumping. The material sprayed in the high-temperature zone heats up rapidly, causing enzyme inactivation and promoting the precipitation of small molecule peptides. The pore size increases radially from the inside to the outside (inner ring 1-2mm → middle ring 3-5mm → outer ring 5-8mm), matching the reaction requirements of the temperature control zone. Combined with the tilt adjustment of the temperature control linkage mechanism (320), the synergistic optimization of zoned temperature control and material distribution is achieved.

[0221] Dynamic tilt angle adjustment: The temperature control linkage mechanism 320 dynamically adjusts the tilt angle according to the signal from the online viscometer 660 to adapt to the distribution requirements of different reaction stages (such as increasing the tilt angle in the low-temperature zone to promote diffusion, and decreasing the tilt angle in the high-temperature zone to prevent local overheating). In this application, a total of 8 waterproof servo motors 321 are provided. Each waterproof servo motor 321 controls the angle of the arc-shaped distribution plate 310 independently. For convenient zoned control, the top control group uniformly controls 2 servo motors to drive the arc-shaped distribution plate 310 in the top temperature control zone, the middle control group uniformly controls 3 servo motors to drive the arc-shaped distribution plate 310 in the middle temperature control zone, and the bottom control group uniformly controls 3 servo motors to drive the arc-shaped distribution plate 310 in the bottom temperature control zone. Each control group receives PLC instructions through the PROFIBUS-DP bus to achieve synchronous action within the group. This is a common technical means in the prior art and will not be described in detail here.

[0222] Temperature control linkage mechanism 320

[0223] Structural features:

[0224] It includes a waterproof servo motor 321, a drive gear 322, and a driven gear 323 embedded in the side wall of the hollow spiral lifting plate 300.

[0225] A polytetrafluoroethylene sealing ring is provided between the waterproof servo motor 321 and the hinge shaft 311 to prevent leakage.

[0226] Functions and uses:

[0227] Closed-loop control: Receives signals from temperature sensor 610 and independently drives the tilt angle of each layer of arc-shaped distribution plate 310 to achieve coordinated optimization of zoned temperature control and material distribution.

[0228] When viscosity increases (e.g., due to enzyme aggregation), the temperature control linkage mechanism 320 increases the tilt angle of the arc-shaped distribution plate 310, enhances the injection force of the small holes, and reduces viscosity.

[0229] When viscosity decreases (e.g., due to excessive hydrolysis), reduce the tilt angle to maintain a stable distribution.

[0230] Top low-temperature zone: Increase the inclination angle of the inner ring orifice and extend the injection path to ensure sufficient activation of the low-temperature zone.

[0231] High-temperature zone at the bottom: Reduce the inclination angle of the outer ring large orifice, shorten the injection path, and avoid local overheating that could lead to peptide chain breakage.

[0232] Anti-interference design: The waterproof servo motor 321 and PTFE sealing ring ensure stable operation in high humidity environments.

[0233] Dynamic material conveying channel 330

[0234] Structural features:

[0235] It includes an axial vertical connecting groove 341 and an eccentric elliptical groove 342 within the central positioning shaft 200, as well as a waist-shaped through groove 343 on the side wall of the hollow spiral lifting plate 300.

[0236] The waist-shaped through groove 343 and the elliptical groove 342 are dynamically sealed together to adapt to the movement of the hollow spiral lifting plate 300.

[0237] Functions and uses:

[0238] Continuous feeding: The annular cavity 710 of the rotary interface seat 700 is connected to an external conduit to ensure that the feed pump continuously inputs materials.

[0239] Dynamic sealing: The sliding seal design of the waist-shaped through groove 343 and the elliptical groove 342 prevents material leakage during lifting and lowering.

[0240] Gradient dispersion wells 312: The pore size of the arc-shaped distribution plate 310 increases from the inside to the outside. The inner ring of small pores precisely sprays the enzyme solution to the low temperature zone, the middle ring achieves balanced diffusion, and the outer ring of large pores accelerates mixing, matching the reaction requirements of different temperature control zones.

[0241] Composite drive unit

[0242] Planetary Gear Set 500:

[0243] Power distribution: The servo motor 520 drives the planetary carrier 530 to rotate the planetary gear 560. The sun gear 540 and the gear ring 550 work together to drive the reciprocating shaft 410 to rotate. At the same time, the hollow spiral lifting plate 300 is vertically reciprocated through the threaded groove 421 and the titanium alloy connecting plate 420.

[0244] Motion coupling: The combined motion of rotation and lifting creates a three-dimensional circulating flow of materials within the tank, preventing stratification and clumping, and improving the uniformity of enzymatic hydrolysis.

[0245] 420 titanium alloy connecting plate: corrosion resistant and lightweight, suitable for high-frequency reciprocating motion;

[0246] Limit block 431 prevents overtravel during lifting and damage to the structure.

[0247] Multi-parameter sensing system 600

[0248] Closed-loop control:

[0249] Temperature sensor 610: Layered monitoring ensures independent adjustment of temperature control zones.

[0250] pH sensor 620: symmetrically arranged at the midpoint of the axis, it provides real-time feedback on acidity and alkalinity, triggering automatic neutralization of the acid / alkali solution tube.

[0251] Dissolved oxygen sensor 630: Immersion type to detect the oxygen content in the liquid phase. When the oxygen content is below the threshold, an inert gas (such as nitrogen) is injected through the gas tube 106 to isolate oxygen.

[0252] Online viscometer 660: The signal is directly connected to the temperature control linkage mechanism 320 to dynamically adjust the tilt angle of the arc distribution plate 310 to maintain the optimal viscosity (such as reducing viscosity to prevent enzyme aggregation).

[0253] Pressure sensor 640: Monitors exhaust pipe pressure to prevent overpressure.

[0254] The 650 weighing sensor tracks the total amount of material and controls the feeding accuracy.

[0255] Rotary interface socket 700

[0256] Dynamic sealing feeding:

[0257] Annular cavity 710: The rotating sleeve and the external conduit are dynamically connected through a fluororubber sealing ring, ensuring that the feed pump continuously delivers enzyme solution and sea cucumber homogenate to the dynamic feed channel 330.

[0258] Sealing design: The PTFE sealing ring dynamically matches the waist-shaped through groove 333 to prevent leakage while adapting to the movement of the hollow spiral lifting plate 300.

[0259] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0260] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A sea cucumber peptide enzymatic hydrolysis device for enhancing immunity, characterized in that: The application relates to a partition-temperature-control enzyme hydrolysis tank (100), the inner wall of which is divided into a top temperature-control area, a middle temperature-control area and a bottom temperature-control area along an axial direction, each temperature-control area is provided with an independent spiral heat exchange coil (110) and a temperature sensor (610), a center positioning shaft (200) is installed on the axial line of the partition-temperature-control enzyme hydrolysis tank (100) through a sealing bearing (210), at least two hollow spiral lifting plates (300) are slidingly sleeved on the center positioning shaft (200) and the outer wall of each hollow spiral lifting plate (300) is hingedly connected with an arc-shaped distribution plate (310), the surface of the arc-shaped distribution plate (310) is uniformly distributed with gradient dispersion holes (312), the hole diameters of the gradient dispersion holes (312) increase from the inside to the outside along the radial direction to form three circles of holes, a temperature-control linkage mechanism (320) is arranged in the control groups corresponding to the temperature-control areas to independently adjust the inclination angles of the corresponding levels of arc-shaped distribution plates (310), a dynamic material conveying channel (330) penetrates through the center positioning shaft (200) to the arc-shaped distribution plate (310), a composite driving unit (400) synchronously drives the rotation and lifting of a reciprocating driving shaft (410) and the hollow spiral lifting plate (300) through a planetary gear set (500), the composite driving unit (400) comprises a titanium alloy connecting plate (420) fixed on the inner wall of the hollow spiral lifting plate (300), the titanium alloy connecting plate (420) is lifted in a central lifting area (220) of the center positioning shaft (200), a threaded groove (421) penetrates through the titanium alloy connecting plate (420), the reciprocating driving shaft (410) is matched with the threaded groove (421), limiting blocks (430) are arranged at both ends of the lifting stroke, a multi-parameter sensing system (600) is integrated with a temperature sensor (610), a PH sensor (620), a dissolved oxygen sensor (630), a pressure sensor (640), a weighing sensor (650) and an online viscosity meter (660), a rotary interface seat (700) is fixed on the top of the partition-temperature-control enzyme hydrolysis tank (100) and is slidingly sleeved with the center positioning shaft (200), an internal annular cavity (710) of the rotary interface seat (700) is sealingly connected with the dynamic material conveying channel (330) and an external conduit (730), the rotary interface seat (700) comprises a sleeve (720) which is connected with the top of the partition-temperature-control enzyme hydrolysis tank (100) through a flange, the annular cavity (710) is arranged on the inner wall of the sleeve (720) and is provided with a dynamic sealing ring (711), the external conduit (730) penetrates through the sleeve (720) in the radial direction to communicate with the annular cavity (710), wherein the online viscosity meter (660) is signal-connected with the temperature-control linkage mechanism (320) to dynamically adjust the inclination angles of the arc-shaped distribution plates (310). The temperature-control linkage mechanism (320) comprises a waterproof servo motor (321) embedded in the side wall of the hollow spiral lifting plate (300), a driving gear (322) arranged on the output shaft of the waterproof servo motor (321), and a driven gear (323) fixed on the hinged shaft (311) of the arc-shaped distribution plate (310) and meshed with the driving gear (322). The planetary gear set (500) comprises a fixed support (510), a planet carrier (530) driven by a servo motor (520), a sun gear (540) fixed with the reciprocating driving shaft (410), and a gear ring (550) fixed with the center positioning shaft (200). ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ 2. The sea cucumber peptide enzymolysis device for improving immunity according to claim 1, characterized in that: ​ ​ ​ ​ 3. The sea cucumber peptide enzymolysis device for improving immunity according to claim 1, characterized in that: ​ ​ ​ ​ ​ Hardened planet gears (560) engaging with the sun gear (540) and the ring gear (550).

4. The sea cucumber peptide enzymolysis device for improving immunity according to claim 1, characterized in that: In the multi-parameter sensing system (600): The temperature sensors (610) are vertically arranged at the center of each temperature control zone; The pH sensors (620) are symmetrically arranged at the axial midpoint of the partitioned temperature control enzymolysis tank (100); The dissolved oxygen sensors (630) are submerged below the liquid surface; The pressure sensors (640) are arranged in the exhaust pipeline; The weighing sensors (650) are integrated at the bottom of the partitioned temperature control enzymolysis tank (100).

5. The sea cucumber peptide enzymolysis device for improving immunity according to claim 1, characterized in that: The dynamic material conveying channel (330) comprises: An axial main groove (331) in the center positioning shaft (200); An elliptical groove (332) connected to the axial main groove (331); A waist-shaped through groove (333) in the sidewall of the hollow spiral lifting plate (300), which dynamically seals with the elliptical groove (332).

6. The sea cucumber peptide enzymolysis device for improving immunity according to claim 1, characterized in that: The partitioned temperature control enzymolysis tank (100) comprises: A double-layer insulation tank body (120); A top cover (130) integrated with coaxially nested auxiliary material pipes (131) and gas pipes (132); An inverted conical bottom cover (140) with a discharge pipe (141) coaxially arranged with the center positioning shaft (200); A bottom auxiliary fixing plate (150) fixed to the inner bottom of the double-layer insulation tank body (120) by circumferentially distributed fixing rods (160), and coaxially movably connected with the center positioning shaft (200) and the reciprocating drive shaft (410); Supporting legs (170) arranged in a circumferential array on the sidewall of the double-layer insulation tank body (120). 7.The method of using the sea cucumber peptide hydrolysis device for improving immunity according to claim 6, characterized in that, The method comprises the following steps: S1, parameter setting Setting the target temperature values of each temperature control zone, the material ratio parameters of the protease solution and the sea cucumber homogenate, and the initial rotation speed in the control system during the enzymolysis process; S2, coordinated feeding and dynamic mixing Synchronously inputting the protease solution and the sea cucumber tissue homogenate through the dynamic material conveying channel (330), and driving the composite drive unit (400) to perform the following composite motion: The planetary gear set (500) drives the center positioning shaft (200) to continuously rotate; The reciprocating drive shaft (410) drives the hollow spiral lifting plate (300) to perform periodic lifting motion; The material is distributed in the three-dimensional space of the partitioned temperature control enzymolysis tank (100) through the gradient dispersion holes (312) of the arc-shaped distribution plate (310) during the rotation and lifting process; S3, real-time regulation of distribution state Based on the real-time detection of the mixed liquid viscosity data by the online viscometer (660), the inclination angles of each layer of the arc-shaped distribution plate (310) are dynamically adjusted through the temperature control linkage mechanism (320); At the same time, according to the feedback of the temperature sensors of each temperature control zone, the heat exchange intensity of the corresponding region spiral heat exchange coil (110) is independently controlled; S4, closed-loop control of environmental parameters When the pH sensor (620) detects that the medium pH deviates from the set range, the acid pipe or the alkali pipe is selectively opened for neutralization adjustment; When the dissolved oxygen sensor (630) detects a value lower than the critical threshold, inert gas is injected into the liquid through the gas pipe (132); S5, termination of reaction and discharge After the set enzymolysis time is reached, the temperature in the partitioned temperature control enzymolysis tank (100) is raised to inactivate the enzyme, and then the discharge pipe (141) of the inverted conical bottom cover (140) is opened to output the enzymolysis product.

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