Reaction device for gene detection sample

Abstract

The invention provides a gene detection sample reaction device, and belongs to the technical field of gene detection.The gene detection sample reaction device comprises a bottom box, a reaction barrel, a driving assembly, a piston assembly, an elastic supporting assembly, a first limiting assembly and a second limiting assembly, a support plate is fixedly arranged on the upper portion of the bottom box, and the reaction barrel is arranged above the bottom box; the driving assembly is installed on the support plate, the elastic supporting assembly is arranged on the side of the reaction barrel, the driving assembly is matched with the elastic supporting assembly to drive the reaction barrel to move up and down in a reciprocating mode, and one end of the piston assembly extends into the reaction barrel. Compared with the prior art, the embodiment of the invention has the advantages that when the gene sample liquid reacts, automatic liquid filling of the gene sample liquid can be realized, so that the reaction efficiency is improved, the gene sample liquid cannot be exposed in the external environment in the liquid filling process, the probability that the gene sample liquid is polluted is effectively reduced, and the reaction effect of the gene sample liquid is improved.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of gene detection, and specifically relates to a reaction device for gene detection samples. BACKGROUND

[0002] At present, gene samples need to be cultured in vitro during gene detection, and a bioreactor, as a key device for the whole culture process, provides a suitable growth environment for sample liquid cells, so that the cells can proliferate rapidly and form the required biological tissue products.

[0003] In the prior art, when the reactor is used for culturing gene sample liquid, the gene sample liquid needs to be manually added to the reactor container, and then vibration and stirring and other means are used to make the gene sample liquid shake in the reactor container, so as to accelerate the reaction speed of the gene sample liquid. However, the process of adding the gene sample liquid to the reactor container is easy to cause the gene sample liquid to be exposed to the environment, thereby increasing the probability of contamination of the gene sample liquid and affecting the subsequent reaction of the gene sample liquid. SUMMARY

[0004] In view of the above problems in the prior art, the technical problem to be solved by the embodiments of the present application is to provide a reaction device for gene detection samples.

[0005] To solve the above technical problems, the present application provides the following technical scheme:

[0006] A reaction device for gene detection samples, comprising a bottom box, a reaction barrel, a driving assembly, a piston assembly, an elastic support assembly, a first limiting assembly and a second limiting assembly,

[0007] A support plate is fixedly arranged on the upper part of the bottom box, and the reaction barrel is arranged above the bottom box,

[0008] The driving assembly is installed on the support plate, the elastic support assembly is arranged on the side of the reaction barrel, and the driving assembly cooperates with the elastic support assembly to drive the reaction barrel to move up and down reciprocally,

[0009] One end of the piston assembly extends into the reaction barrel, and the other end extends above the reaction barrel,

[0010] The first limiting assembly is arranged in the reaction barrel, and the second limiting assembly is arranged above the reaction barrel. When the reaction barrel moves up and down reciprocally, the first limiting assembly cooperates with the second limiting assembly to drive the piston assembly to move upward relative to the reaction barrel, so as to draw the external gene sample liquid into the reaction barrel.

[0011] As a further improved scheme of the present application, a top shaft is fixedly arranged on the outer wall of the reaction barrel,

[0012] The driving assembly comprises a motor and an eccentric wheel,

[0013] The motor is fixedly installed on the side wall of the support plate, the eccentric wheel is arranged on the output end of the motor, and the eccentric wheel acts on the bottom of the top shaft,

[0014] The elastic support assembly comprises a second guide rod, a sliding sleeve, a connecting rod, a first elastic member and an end block,

[0015] The second guide rod is fixedly arranged on the upper part of the bottom box and vertically distributed, the end block is fixedly arranged on the upper end of the second guide rod, the sliding sleeve is slidably arranged on the outside of the second guide rod, one end of the connecting rod is fixedly connected with the outer wall of the reaction bucket, the other end is fixedly connected with the sliding sleeve, one end of the first elastic member is connected with the end block, and the other end is connected with the sliding sleeve, so as to provide elastic support for the sliding sleeve.

[0016] As a further improvement of the present application: the piston assembly comprises a piston plate and a piston rod,

[0017] The piston plate is movably arranged in the reaction bucket, one end of the piston rod is connected with the upper part of the piston plate, and the other end extends from the top wall of the reaction bucket to above the reaction bucket.

[0018] As a further improvement of the present application: a liquid inlet hard pipe is fixedly arranged on the outer wall of the reaction bucket, and the end of the liquid inlet hard pipe away from the reaction bucket is connected with an external gene sample liquid storage box through a liquid inlet soft pipe.

[0019] As a further improvement of the present application: a limiting frame is fixedly arranged on the upper end of the support plate, the end of the limiting frame away from the support plate extends to above the reaction bucket, the piston rod passes through the middle of the limiting frame, a first guide rod vertically distributed is fixedly arranged in the reaction bucket, the first guide rod penetrates the piston plate and movably cooperates with the piston plate,

[0020] The first limiting assembly comprises a first inclined tooth piece, a second elastic member and an extension rod,

[0021] The first inclined tooth piece is provided with a plurality of groups, a plurality of the first inclined tooth pieces are hingedly arranged on the side wall of the first guide rod and are sequentially and spacedly distributed along the length direction of the first guide rod, the inclined direction of a plurality of the first inclined tooth pieces is upward, one side of each group of the first inclined tooth pieces is connected with the first guide rod through a group of the second elastic members, one end of the extension rod extends to between two adjacent groups of the first inclined tooth pieces, and the other end is fixedly connected with the piston rod,

[0022] The second limiting assembly comprises a second inclined tooth piece and a third elastic member,

[0023] The second inclined tooth pieces are arranged on the side wall of the piston rod and are distributed along the length direction of the piston rod, and the inclined directions of the second inclined tooth pieces are downward, and one side of each group of the second inclined tooth pieces is connected with the piston rod through one group of the third elastic members.

[0024] As a further improved scheme of the present application, the bottom of the reaction barrel is provided with a liquid outlet pipe, the liquid outlet pipe is provided with a valve inside, the liquid inlet pipe is provided with a one-way valve inside, and the piston rod is rotationally connected with the piston plate.

[0025] As a further improved scheme of the present application, the upper part of the piston plate is further provided with a reset assembly, and the reset assembly is used for limiting the reverse rotation angle of the piston rod.

[0026] As a further improved scheme of the present application, the reset assembly comprises a rack, a first support, a sliding block, a gear, a sixth elastic member, a guide rail and a second support,

[0027] The guide rail is fixedly arranged on the upper part of the piston plate, the first support and the second support are respectively fixedly arranged on opposite ends of the guide rail, the sliding block is in sliding fit with the guide rail, one end of the sixth elastic member is connected with the second support, the other end is connected with the sliding block, and the sixth elastic member is used for providing elastic support for the sliding block, the rack is fixedly arranged on the upper part of the sliding block, and the gear is fixedly arranged on the side wall of the piston rod and is in mesh with the rack.

[0028] As a further improved scheme of the present application, the inside of the reaction barrel is further provided with an oscillation assembly, and when the reaction barrel moves up and down to drive the gene sample liquid to shake, the oscillation assembly is used for oscillating the gene sample liquid.

[0029] As a further improved scheme of the present application, the oscillation assembly comprises a fourth elastic member, an oscillation block and a fifth elastic member,

[0030] The oscillation block is provided with a plurality of groups, the oscillation block is movably sleeved on the outside of the first guide rod, the uppermost group of the oscillation block is connected with the bottom wall of the piston plate through the fourth elastic member, and the adjacent two groups of the oscillation block are connected through the fifth elastic member.

[0031] Compared with the prior art, the present application has the following advantages:

[0032] When the reaction needs to be carried out on the gene sample liquid, the driving assembly can be used in cooperation with the elastic supporting assembly to drive the reaction barrel to move up and down reciprocatingly. When the reaction barrel moves up and down reciprocatingly, the first limiting assembly cooperates with the second limiting assembly to drive the piston assembly to move up relative to the reaction barrel, so as to draw the external gene sample liquid into the reaction barrel, and the automatic liquid filling of the gene sample liquid is realized. When the gene sample liquid is drawn into the reaction barrel, the gene sample liquid is shaken in the reaction barrel with the up and down movement of the reaction barrel, so as to accelerate the reaction speed of the gene sample liquid. Compared with the prior art, the automatic liquid filling of the gene sample liquid can be realized when the gene sample liquid is reacted, so as to improve the reaction efficiency. In addition, the gene sample liquid will not be exposed to the external environment during the liquid filling process, so as to effectively reduce the probability of contamination of the gene sample liquid and improve the reaction effect of the gene sample liquid. BRIEF DESCRIPTION OF DRAWINGS

[0033] Figure 1 Structure diagram of a reaction device for a gene detection sample Figure 1 ;

[0034] Figure 2 Structure diagram of a reaction device for a gene detection sample Figure 2 ;

[0035] Figure 3 Structure diagram of a reaction device for a gene detection sample Figure 3 ;

[0036] Figure 4 Figure 1 Enlarged view of region A in FIG. 1;

[0037] Figure 5 Figure 1 Enlarged view of region B in FIG. 1;

[0038] Figure 6 Figure 1 Enlarged view of region C in FIG. 1;

[0039] Figure 7 Figure 2 Enlarged view of region D in FIG. 1;

[0040] ​​​​In the figure: 10 - bottom box, 101 - support plate, 102 - limiting frame, 20 - reaction bucket, 201 - liquid inlet hose, 202 - liquid inlet hard pipe, 203 - liquid outlet pipe, 204 - top shaft, 205 - first guide rod, 30 - drive assembly, 301 - motor, 302 - eccentric wheel, 40 - piston assembly, 401 - piston plate, 402 - piston rod, 50 - elastic support assembly, 501 - second guide rod, 502 - sliding sleeve, 503 - connecting rod, 504 - first elastic member, 505 - end block, 60 - first limiting assembly, 601 - first inclined tooth piece, 602 - second elastic member, 603 - extension rod, 70 - second limiting assembly, 701 - second inclined tooth piece, 702 - third elastic member, 80 - oscillation assembly, 801 - fourth elastic member, 802 - oscillation block, 803 - fifth elastic member, 90 - reset assembly, 901 - rack, 902 - first support, 903 - sliding block, 904 - gear, 905 - sixth elastic member, 906 - guide rail, 907 - second support. DETAILED DESCRIPTION

[0041] The technical solutions of the present application will be further described in detail below in combination with specific embodiments.

[0042] The embodiments of the present application are described in detail below, and examples of the embodiments are shown in the drawings, wherein the same or similar reference signs represent the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the drawings are exemplary and are only used to explain the present application, and cannot be understood as a limitation of the present application.

[0043] In the description of the present application, it should be understood that the terms "center", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation of the present application.

[0044] In the description of the present application, it should be noted that unless otherwise explicitly specified and limited, the terms "mounting", "connecting", "connecting", "setting" should be understood broadly, for example, it can be fixedly connected, set, or detachably connected, set, or integrally connected, set. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0045] Please refer to Figure 1 , Figure 2 and Figure 3The embodiment provides a reaction device for gene detection sample, which comprises a bottom box 10, a reaction barrel 20, a driving assembly 30, a piston assembly 40, an elastic support assembly 50, a first limiting assembly 60 and a second limiting assembly 70, a support plate 101 is fixedly arranged on the upper portion of the bottom box 10, the reaction barrel 20 is arranged above the bottom box 10, the driving assembly 30 is installed on the support plate 101, the elastic support assembly 50 is arranged on the side of the reaction barrel 20, the driving assembly 30 cooperates with the elastic support assembly 50 to drive the reaction barrel 20 to move up and down reciprocatingly, one end of the piston assembly 40 extends to the inside of the reaction barrel 20, and the other end extends above the reaction barrel 20, the first limiting assembly 60 is arranged in the reaction barrel 20, and the second limiting assembly 70 is arranged above the reaction barrel 20, when the reaction barrel 20 moves up and down reciprocatingly, the first limiting assembly 60 cooperates with the second limiting assembly 70 to drive the piston assembly 40 to move upwards relative to the reaction barrel 20, so that the external gene sample liquid is drawn into the inside of the reaction barrel 20.

[0046] When the reaction needs to be carried out on the gene sample liquid, the driving assembly 30 cooperates with the elastic support assembly 50 to drive the reaction barrel 20 to move up and down reciprocatingly, when the reaction barrel 20 moves up and down reciprocatingly, the first limiting assembly 60 cooperates with the second limiting assembly 70 to drive the piston assembly 40 to move upwards relative to the reaction barrel 20, so that the external gene sample liquid is drawn into the inside of the reaction barrel 20, and the automatic liquid filling of the gene sample liquid is realized, when the gene sample liquid is drawn into the inside of the reaction barrel 20, the gene sample liquid is shaken in the inside of the reaction barrel 20 along with the up-and-down movement of the reaction barrel 20, so that the reaction speed of the gene sample liquid is accelerated.

[0047] Please refer to Figure 1 and Figure 3In one embodiment, the outer wall of the reaction bucket 20 is fixedly provided with a top shaft 204, the driving assembly 30 comprises a motor 301 and an eccentric wheel 302, the motor 301 is fixedly installed on the side wall of the support plate 101, the eccentric wheel 302 is arranged at the output end of the motor 301, the eccentric wheel 302 acts on the bottom of the top shaft 204, the elastic support assembly 50 comprises a second guide rod 501, a sliding sleeve 502, a connecting rod 503, a first elastic member 504 and an end block 505, the second guide rod 501 is fixedly arranged on the upper part of the bottom box 10 and vertically distributed, the end block 505 is fixedly arranged on the upper end of the second guide rod 501, the sliding sleeve 502 is slidably arranged outside the second guide rod 501, one end of the connecting rod 503 is fixedly connected with the outer wall of the reaction bucket 20, the other end is fixedly connected with the sliding sleeve 502, one end of the first elastic member 504 is connected with the end block 505, the other end is connected with the sliding sleeve 502, and the first elastic member 504 is arranged to provide elastic support for the sliding sleeve 502.

[0048] When it is necessary to react on the gene sample liquid, the motor 301 is started, the motor 301 drives the eccentric wheel 302 to rotate, the eccentric wheel 302 acts on the top shaft 204 when rotating, and the top shaft 204 is pushed upward, so that the reaction bucket 20 moves upward, when the reaction bucket 20 moves upward, the sliding sleeve 502 is driven by the connecting rod 503 to slide upward along the second guide rod 501, at this time, the first elastic member 504 is compressed under stress, with the continuous rotation of the eccentric wheel 302, the top shaft 204 is no longer pushed upward by the eccentric wheel 302, the first elastic member 504 drives the sliding sleeve 502 to slide downward along the outside of the second guide rod 501, the sliding sleeve 502 drives the reaction bucket 20 to move downward through the connecting rod 503, and the up-down movement of the reaction bucket 20 is realized through such reciprocating circulation, and then the gene sample liquid entering the inside of the reaction bucket 20 is shaken.

[0049] Please refer to Figure 1 In one embodiment, the piston assembly 40 comprises a piston plate 401 and a piston rod 402, the piston plate 401 is movably arranged in the inside of the reaction bucket 20, one end of the piston rod 402 is connected with the upper part of the piston plate 401, and the other end extends from the top wall of the reaction bucket 20 to above the reaction bucket 20.

[0050] When the motor 301 drives the eccentric wheel 302 to rotate and drives the reaction bucket 20 to move up and down in cooperation with the first elastic member 504, the first limiting assembly 60 cooperates with the second limiting assembly 70 to drive the piston plate 401 and the piston rod 402 to move upward as a whole compared with the reaction bucket 20, the piston plate 401 moves upward to draw the external gene sample liquid into the inside of the reaction bucket 20, and the automatic liquid filling of the gene sample liquid is realized.

[0051] Please refer to Figure 1 andFigure 2 In one embodiment, the reaction barrel 20 outer wall is fixedly provided with a liquid inlet hard pipe 202, and one end of the liquid inlet hard pipe 202 is connected to an external gene sample liquid storage box (not shown in the figure) through a liquid inlet soft pipe 201.

[0052] When the piston plate 401 moves upwards along the inside of the reaction barrel 20, the piston plate 401 can draw the gene sample liquid in the external gene sample liquid storage box into the inside of the reaction barrel 20 through the liquid inlet soft pipe 201 and the liquid inlet hard pipe 202 by virtue of negative pressure, thereby realizing automatic liquid loading of the gene sample liquid.

[0053] Please refer to Figure 1 , Figure 6 and Figure 7 In one embodiment, the support plate 101 upper end is fixedly provided with a limiting frame 102, the limiting frame 102 extends to above the reaction barrel 20 from one end of the support plate 101, the piston rod 402 passes through the middle of the limiting frame 102, the inside of the reaction barrel 20 is fixedly provided with vertically distributed first guide rods 205, the first guide rods 205 pass through and movably cooperate with the piston plate 401, the first limiting assembly 60 includes first inclined teeth 601, second elastic members 602 and extension rods 603, the first inclined teeth 601 are provided in several groups, the first inclined teeth 601 are hingedly arranged on the side wall of the first guide rods 205 and are sequentially and spacedly distributed along the length direction of the first guide rods 205, the inclination direction of the first inclined teeth 601 is upward, one side of each group of the first inclined teeth 601 is connected with the first guide rods 205 through one group of the second elastic members 602, one end of the extension rod 603 extends to between two adjacent groups of the first inclined teeth 601, and the other end is fixedly connected with the piston rod 402, the second limiting assembly 70 includes second inclined teeth 701 and third elastic members 702, the second inclined teeth 701 are provided in several groups, the second inclined teeth 701 are hingedly arranged on the side wall of the piston rod 402 and are sequentially and spacedly distributed along the length direction of the piston rod 402, the inclination direction of the second inclined teeth 701 is downward, and one side of each group of the second inclined teeth 701 is connected with the piston rod 402 through one group of the third elastic members 702.

[0054] When the eccentric wheel 302 is pushed upward for the top shaft 204 to drive the reaction bucket 20 to move upward, the reaction bucket 20 drives the guide rod 205 and the first inclined tooth piece 601 to move upward synchronously, at this time, the first inclined tooth piece 601 located below the extension rod 603 acts on the extension rod 603, thereby pushing the extension rod 603 to make the piston rod 402 and the piston plate 401 move upward synchronously, the piston plate 401 does not move relative to the reaction bucket 20, when the piston rod 402 moves upward, the second inclined tooth piece 701 moves upward, a certain group of second inclined tooth pieces 701 passes from the inside of the limiting frame 102 and acts on the upper part of the limiting frame 102 under the support of the third elastic member 703, and when the first elastic member 504 pushes the sliding sleeve 502 to slide downward along the second guide rod 501 to make the reaction bucket 20 move downward, because a certain group of second inclined tooth pieces 701 acts on the upper part of the limiting frame 102, the piston rod 402 and the piston plate 401 cannot move downward with the reaction bucket 20, that is, the piston plate 401 moves upward along the inside of the reaction bucket 20 compared with the reaction bucket 20, in this way, with the continuous upward and downward movement of the reaction bucket 20, the piston plate 401 continuously moves upward along the inside of the reaction bucket 20, the piston plate 401 continuously extracts the gene sample liquid in the external gene sample liquid storage box to the inside of the reaction bucket 20 through the liquid inlet hose 201 and the liquid inlet hard pipe 202, to realize the automatic liquid loading of the gene sample liquid, when the gene sample liquid in the inside of the reaction bucket 20 has a certain amount, a certain group of second inclined tooth pieces 701 located at the bottom passes from the inside of the limiting frame 102 and acts on the upper part of the limiting frame 102, at this time, with the continuous upward and downward movement of the reaction bucket 20, the piston plate 401 and the piston rod 402 move upward and downward synchronously with the reaction bucket 20, the external gene sample liquid stops entering the inside of the reaction bucket 20, at this time, the quantified gene sample liquid in the inside of the reaction bucket 20 moves upward and downward with the reaction bucket 20 to shake.

[0055] Please refer to Figure 2 In one embodiment, the bottom of the reaction bucket 20 is provided with a liquid outlet pipe 203, the inside of the liquid outlet pipe 203 is provided with a valve (not shown in the figure), the inside of the liquid inlet hard pipe 202 is provided with a one-way valve (not shown in the figure), and the piston rod 402 is rotationally connected with the piston plate 401.

[0056] When the piston plate 401 moves upward along the inside of the reaction barrel 20, the one-way valve inside the hard liquid inlet pipe 202 is opened, and the gene sample liquid in the gene sample liquid storage box is extracted into the reaction barrel 20 through the soft liquid inlet pipe 201 and the hard liquid inlet pipe 202. When the gene sample liquid is shaken in the reaction barrel 20 and the reaction is completed, the valve inside the liquid outlet pipe 203 is opened to discharge the gene sample liquid in the reaction barrel 20. Then, the piston rod 402 is rotated, and the piston rod 402 drives the second inclined teeth 701 and the extension rod 603 to rotate synchronously. When the second inclined teeth 701 rotate, they fall into the inside of the limiting frame 102. When the extension rod 603 rotates, it is removed from between the adjacent two groups of first inclined teeth 601. Then, the piston rod 402 is pushed downward, and the piston rod 402 drives the second inclined teeth 701, the piston plate 401, and the extension rod 603 to move downward. When the second inclined teeth 701 move downward, they move smoothly from the inside of the limiting frame 102. When the piston plate 401 moves downward, it moves to the bottom of the reaction barrel 20 for resetting. Then, the piston rod 402 is reversely rotated, and the piston rod 402 drives the second inclined teeth 701 and the extension rod 603 to reversely rotate synchronously. When the second inclined teeth 701 reversely rotate, they act on the bottom of the limiting frame 102 again. When the extension rod 603 reversely rotates, one end of the extension rod 603 extends to between the adjacent two groups of first inclined teeth 601 again, so that the second inclined teeth 701 and the extension rod 603 are reset.

[0057] When the piston rod 402 is reversely rotated, in order to ensure that one end of the extension rod 603 can accurately extend to between the adjacent two groups of first inclined teeth 601, and to ensure that the second inclined teeth 701 can accurately act on the bottom of the limiting frame 102, please refer to Figure 1 In an embodiment, the upper part of the piston plate 402 is further provided with a resetting assembly 90, which is used to limit the reverse rotation angle of the piston rod 402, so that the piston rod 402 can drive the second inclined teeth 701 and the extension rod 603 to reset accurately.

[0058] Please refer to Figure 4 In an embodiment, the resetting assembly 90 includes a rack 901, a first support 902, a sliding block 903, a gear 904, a sixth elastic member 905, a guide rail 906, and a second support 907. The guide rail 906 is fixedly arranged on the upper part of the piston plate 401. The first support 902 and the second support 907 are fixedly arranged at opposite ends of the guide rail 906, respectively. The sliding block 903 is in sliding fit with the guide rail 906. One end of the sixth elastic member 905 is connected with the second support 907, and the other end is connected with the sliding block 903, which is used to provide elastic support for the sliding block 903. The rack 901 is fixedly arranged on the upper part of the sliding block 903. The gear 904 is fixedly arranged on the side wall of the piston rod 402 and is in mesh with the rack 901.

[0059] Initially, the slider 903 is in close contact with the first support 902 under the support of the sixth elastic member 905. When the piston rod 402 is rotated to rotate the second inclined teeth 701 to the inside area of the limiting frame 102 and to remove the one end of the extension rod 603 from between the two adjacent groups of first inclined teeth 601, the piston rod 402 drives the gear 904 to rotate synchronously. When the gear 904 rotates, it drives the slider 903 to slide along the guide rail 906 through the meshing action with the rack 901. The slider 903 moves away from the first support 902, and the sixth elastic member 905 is compressed. After the piston plate 401 is reset, the staff can reverse rotate the piston rod 402 to drive the second inclined teeth 701, the extension rod 603 and the gear 904 to reverse rotate synchronously. When the gear 904 reverses, it drives the slider 903 to slide reversely along the guide rail 906 through the reverse meshing action with the rack 901. Until the slider 903 is in close contact with the first support 902 again, the one end of the extension rod 603 is accurately extended to between the two adjacent groups of first inclined teeth 601, and the second inclined teeth 702 are accurately rotated to below the limiting frame 102.

[0060] Please refer to Figure 2 In an embodiment, the reaction barrel 20 is further provided with an oscillation assembly 80. When the reaction barrel 20 moves up and down to drive the gene sample liquid to shake, the oscillation assembly 80 is used for oscillation treatment of the gene sample liquid, so as to further improve the reaction effect and reaction efficiency of the gene sample liquid.

[0061] Please refer to Figure 7 In an embodiment, the oscillation assembly 80 comprises a fourth elastic member 801, an oscillation block 802 and a fifth elastic member 803. The oscillation block 801 is provided with a plurality of groups. The oscillation block 802 is movably sleeved outside the first guide rod 205. The uppermost group of the oscillation block 802 is connected with the bottom wall of the piston plate 401 through the fourth elastic member 801. The adjacent two groups of the oscillation block 802 are connected through the fifth elastic member 803.

[0062] When the reaction barrel 20 moves up and down to drive the gene sample liquid to shake, the oscillation block 802 can slide up and down outside the first guide rod 205 under the influence of the up and down movement of the reaction barrel 20 and the fourth elastic member 801 and the fifth elastic member 803, so as to oscillate the gene sample liquid.

[0063] In an embodiment, the first elastic member 504, the second elastic member 602, the third elastic member 702, the fourth elastic member 801, the fifth elastic member 803 and the sixth elastic member 905 can be springs or metal springs, which are not limited here.

[0064] When the reaction of the gene sample liquid is needed, the driving assembly 30 is matched with the elastic supporting assembly 50, and then the reaction barrel 20 is driven to move up and down reciprocatingly. When the reaction barrel 20 moves up and down reciprocatingly, the first limiting assembly 60 and the second limiting assembly 70 are matched, and then the piston assembly 40 is driven to move up compared with the reaction barrel 20, so that the gene sample liquid outside is extracted into the reaction barrel 20, and the automatic liquid filling of the gene sample liquid is realized. When the gene sample liquid is extracted into the reaction barrel 20, the gene sample liquid is shaken in the reaction barrel 20 with the up and down movement of the reaction barrel 20, so that the reaction speed of the gene sample liquid is accelerated. Compared with the prior art, when the gene sample liquid is reacted, the automatic liquid filling of the gene sample liquid can be realized, so that the reaction efficiency is improved. During the liquid filling process, the gene sample liquid is not exposed to the external environment, so that the probability of contamination of the gene sample liquid is effectively reduced, and the reaction effect of the gene sample liquid is improved.

[0065] The preferred embodiments of the present application are described in detail above, but the present application is not limited to the above embodiments. Within the knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present application.

Claims

1. A reaction device for a genetic test sample, characterized by, The bottom box (10), the reaction bucket (20), the driving assembly (30), the piston assembly (40), the elastic support assembly (50), the first limiting assembly (60) and the second limiting assembly (70), The bracket plate (101) is fixedly arranged on the upper portion of the bottom box (10), and the reaction bucket (20) is arranged above the bottom box (10), The driving assembly (30) is installed on the bracket plate (101), the elastic support assembly (50) is arranged on the side of the reaction bucket (20), and the driving assembly (30) cooperates with the elastic support assembly (50) to drive the reaction bucket (20) to move up and down reciprocatingly, One end of the piston assembly (40) extends into the reaction bucket (20), and the other end extends above the reaction bucket (20), The first limiting assembly (60) is arranged in the reaction bucket (20), and the second limiting assembly (70) is arranged above the reaction bucket (20), when the reaction bucket (20) moves up and down reciprocatingly, the first limiting assembly (60) cooperates with the second limiting assembly (70) to drive the piston assembly (40) to move up compared with the reaction bucket (20), so as to draw the external gene sample liquid into the reaction bucket (20).

2. The reaction device for a genetic test sample according to claim 1, wherein The top shaft (204) is fixedly arranged on the outer wall of the reaction bucket (20), The driving assembly (30) comprises a motor (301) and an eccentric wheel (302), The motor (301) is fixedly installed on the side wall of the bracket plate (101), the eccentric wheel (302) is arranged on the output end of the motor (301), and the eccentric wheel (302) acts on the bottom of the top shaft (204), The elastic support assembly (50) comprises a second guide rod (501), a sliding sleeve (502), a connecting rod (503), a first elastic member (504) and an end block (505), The second guide rod (501) is fixedly arranged on the upper portion of the bottom box (10) and vertically distributed, the end block (505) is fixedly arranged on the upper end of the second guide rod (501), the sliding sleeve (502) is slidably arranged on the outside of the second guide rod (501), one end of the connecting rod (503) is fixedly connected with the outer wall of the reaction bucket (20), the other end is fixedly connected with the sliding sleeve (502), one end of the first elastic member (504) is connected with the end block (505), and the other end is connected with the sliding sleeve (502), so as to provide elastic support for the sliding sleeve (502).

3. The reaction device for a genetic test sample according to claim 1, wherein The piston assembly (40) comprises a piston plate (401) and a piston rod (402), The piston plate (401) is movably arranged in the reaction bucket (20), one end of the piston rod (402) is connected with the upper portion of the piston plate (401), and the other end extends from the top wall of the reaction bucket (20) to above the reaction bucket (20).

4. The reaction device for a genetic test sample according to claim 3, wherein The liquid inlet hard pipe (202) is fixedly arranged on the outer wall of the reaction bucket (20), and one end of the liquid inlet hard pipe (202) away from the reaction bucket (20) is connected with an external gene sample liquid storage box through a liquid inlet soft pipe (201).

5. The reaction device for a genetic test sample according to claim 3, wherein The support plate (101) is fixedly provided with a limiting frame (102) at the upper end, the limiting frame (102) extends to above the reaction barrel (20) away from the support plate (101), the piston rod (402) passes through the middle of the limiting frame (102), the reaction barrel (20) is fixedly provided with vertically distributed first guide rods (205) inside, the first guide rods (205) penetrate through the piston plate (401) and movably cooperate with the piston plate (401), The first limiting assembly (60) comprises first inclined teeth (601), second elastic members (602) and extension rods (603), The first inclined teeth (601) are provided in groups, the first inclined teeth (601) are hingedly arranged on the side wall of the first guide rod (205) and are sequentially and spacedly distributed along the length direction of the first guide rod (205), the inclined directions of the first inclined teeth (601) are upward, one side of each group of the first inclined teeth (601) is connected with the first guide rod (205) through one group of the second elastic members (602), one end of the extension rod (603) extends to between two adjacent groups of the first inclined teeth (601), and the other end is fixedly connected with the piston rod (402), The second limiting assembly (70) comprises second inclined teeth (701) and third elastic members (702), The second inclined teeth (701) are provided in groups, the second inclined teeth (701) are hingedly arranged on the side wall of the piston rod (402) and are sequentially and spacedly distributed along the length direction of the piston rod (402), the inclined directions of the second inclined teeth (701) are downward, one side of each group of the second inclined teeth (701) is connected with the piston rod (402) through one group of the third elastic members (702).

6. The reaction device for a genetic test sample according to claim 4, wherein The reaction barrel (20) is provided with a liquid outlet pipe (203) at the bottom, the liquid outlet pipe (203) is provided with a valve inside, the liquid inlet pipe (202) is provided with a one-way valve inside, and the piston rod (402) is rotationally connected with the piston plate (401).

7. The reaction device for a genetic test sample according to claim 3, wherein The piston plate (402) is further provided with a reset assembly (90) at the upper portion, and the reset assembly (90) is used for limiting the reverse rotation angle of the piston rod (402).

8. The reaction device for a genetic test sample according to claim 7, wherein The reset assembly (90) comprises a rack (901), a first support (902), a sliding block (903), a gear (904), a sixth elastic member (905), a guide rail (906) and a second support (907), The guide rail (906) is fixedly arranged on the upper portion of the piston plate (401), the first support (902) and the second support (907) are respectively fixedly arranged on opposite ends of the guide rail (906), the sliding block (903) is in sliding fit with the guide rail (906), one end of the sixth elastic member (905) is connected with the second support (907), and the other end is connected with the sliding block (903), so as to provide elastic support for the sliding block (903), and the rack (901) is fixedly arranged on the upper portion of the sliding block (903), and the gear (904) is fixedly arranged on the side wall of the piston rod (402) and is in mesh with the rack (901).

9. The reaction device for a genetic test sample according to claim 5, wherein The reaction barrel (20) is internally provided with an oscillation assembly (80), which is used for oscillation treatment on the gene sample liquid when the reaction barrel (20) moves up and down to drive the gene sample liquid to shake.

10. The reaction device for a genetic test sample according to claim 9, wherein The oscillation assembly (80) comprises a fourth elastic member (801), an oscillation block (802) and a fifth elastic member (803), The oscillation block (801) is provided in several groups, and the oscillation blocks (801) are movably sleeved on the outside of the first guide rod (205), wherein the uppermost group of the oscillation blocks (802) is connected with the bottom wall of the piston plate (401) through the fourth elastic member (801), and the adjacent two groups of the oscillation blocks (802) are connected through the fifth elastic member (803).

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