Stem cell extraction method and device

Abstract

The invention relates to the technical field of stem cell extraction, in particular to a stem cell extraction method and device.The stem cell extraction device comprises an extraction and separation device body, the inner wall of the extraction and separation device body is movably connected with a separation test tube placing rack, and the top of the outer wall of the separation test tube placing rack is fixedly connected with a separation test tube connecting disc; angle adjusting mechanisms are movably connected to the bottom of the outer wall of the separated test tube placement rack in an annular array mode, and test tube temperature monitoring mechanisms are fixedly connected to the inner sides of the multiple angle adjusting mechanisms. Flexible adjustment can be carried out according to different experiment requirements, specifically, after a separated test tube is inserted into a test tube insertion disc, a bidirectional hydraulic push rod is started, so that an outer retracting and expanding plate and a semicircular clamping plate move inwards and are attached to the bottom of the outer wall of the test tube, and when the angle of the test tube is changed, a bottom tray rotating rod rotates, and a bottom tray ascends to stabilize the test tube.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of stem cell extraction, more specifically, the present application relates to a stem cell extraction method and device. BACKGROUND

[0002] Stem cells are cells that have the ability of self-renewal and multi-directional differentiation potential, they play a crucial role in the development, growth and repair processes of living organisms, and the characteristics of stem cells make them have very high value in medical research and clinical applications, especially in the fields of regenerative medicine, tissue engineering and disease treatment, according to their source and differentiation potential, stem cells can be divided into different types such as embryonic stem cells, adult stem cells and induced pluripotent stem cells, each type of stem cell has its unique biological characteristics and application prospects, providing broad possibilities for the development of modern medicine.

[0003] According to the patent document: CN116042372A, a kind of stem cell extraction device and its extraction method are disclosed, including extraction box, the top of extraction box is fixedly connected with sealing cover, the upper side of sealing cover is spirally connected with feeding cover, the inside of extraction box is equipped with separation pipe, the bottom of separation pipe is rotatably connected with rotating plate, the bottom of rotating plate is communicated with extraction pipe, the other side of extraction pipe is communicated with discharge pipe, valve is installed on the outside of extraction pipe;The present application, the rotation of the rotating shaft is driven by starting motor, the rotation of the rotating shaft drives the driving gear to rotate, so that the driven gear rotates at high speed, realizes the centrifugal treatment of liquid, and after centrifugation, stem cells are located in the lower layer, when static, open the valve, the stem cells in the lower layer enter into the inside of discharge pipe through extraction pipe, realize the centralized collection of stem cells, this extraction method can realize the high-purity collection of stem cells, the separation boundary of supernatant and stem cells can be clearly observed by observing extraction pipe, so as to stop in time.

[0004] The angle of the test tube used for centrifugation is fixed in the stem cell extraction and separation device during centrifugation, but in actual operation, although the fixed angle design of the test tube can ensure uniform distribution of liquid during centrifugation and avoid unstable separation effect caused by angle change, it also has certain limitations, for example, the fixed angle may not adapt to the optimal separation conditions of different types of stem cells, resulting in the extraction efficiency of certain specific stem cells being affected. SUMMARY

[0005] In order to overcome the above-mentioned defects of the prior art, the present application provides a stem cell extraction method and device, and the technical problems to be solved by the present application are: in actual operation, although the fixed angle design of the test tube can ensure uniform distribution of the liquid during centrifugation and avoid unstable separation effect caused by angle change, there is also certain limitation, for example, the fixed angle may not adapt to the optimal separation condition of different types of stem cells, resulting in that the extraction efficiency of certain specific stem cells is affected.

[0006] To solve the above technical problems, the technical scheme adopted by the present application is:

[0007] A stem cell extraction method, comprising the following steps:

[0008] Step one: place the cell liquid to be extracted and separated into a specially designed centrifuge tube, and ensure that the liquid volume is moderate to avoid overflow;

[0009] Step two: start the extraction and separation device main body, set appropriate speed and time, and make the cells stratified and precipitated;

[0010] Step three: carefully suck the supernatant with a sterile pipette to avoid disturbing the precipitate layer;

[0011] Step four: transfer the precipitated cells to a new centrifuge tube, add an appropriate amount of washing liquid, and centrifuge again;

[0012] Step five: repeat the washing and centrifugation steps until the cells are pure;

[0013] Step six: collect the pure stem cells and perform subsequent culture or experimental operation.

[0014] The present application also provides a stem cell extraction device, comprising an extraction and separation device main body, the inner wall of the extraction and separation device main body is movably connected with a separation test tube placing rack, the outer wall top of the separation test tube placing rack is fixedly connected with a separation test tube connecting disc, the outer wall bottom of the separation test tube placing rack is movably connected with an angle adjusting mechanism in an annular array, the inner side of multiple angle adjusting mechanisms is fixedly connected with a test tube temperature monitoring mechanism, the separation test tube placing rack comprises a bottom connecting disc, the bottom of the bottom connecting disc is rotatably connected in the middle of the inner wall of the extraction and separation device main body, the outer wall of the bottom connecting disc is sleeved with a track, the inner wall of the side away from the bottom connecting disc of the track is sleeved with a transmission disc, the bottom of the transmission disc is fixedly connected with a motor, the bottom of the transmission disc is fixedly connected with the output end of the motor, and the bottom of the motor is fixedly connected with the inner wall bottom of the extraction and separation device main body away from the bottom connecting disc.

[0015] As a further scheme of the present application: the top of the bottom connecting plate is fixedly connected with a connecting column, the outer wall of the connecting column is arranged with a connecting column sliding groove in the bottom, and the outer wall of the connecting column away from the connecting column sliding grooves is fixedly connected with a sliding groove disc, and the top of the sliding groove disc is arranged with a sliding groove disc sliding groove penetrating through to the bottom.

[0016] As a further scheme of the present application: the inner wall of the bottom connecting plate is fixedly connected with a lead screw motor, the output end of the lead screw motor extends to the inner wall of the connecting column and is fixedly connected with a lead screw, the outer wall of the lead screw is threadedly connected with a transmission shaft, the outer wall of the transmission shaft is fixedly connected with a connecting block in an annular array, the outer side of the connecting block is extended to the outer wall of the connecting column through the connecting column sliding groove and is fixedly connected with a lifting shaft, and the outer wall of the lifting shaft is rotatably connected with a rotating rod in an annular array.

[0017] As a further scheme of the present application: the separation test tube connecting disc comprises a separation test tube connecting disc body, the outer wall of the separation test tube connecting disc body is fixedly connected with a connecting rod in an annular array, the side of the connecting rod away from the separation test tube connecting disc body is fixedly connected with a test tube insertion disc, the outer wall of the side of the connecting rod close to the test tube insertion disc is fixedly connected with a movable bead connecting block, the inner wall of the bottom of the movable bead connecting block is rotatably connected with a movable bead, the outer wall of the movable bead is fixedly connected with a movable bead bottom connecting rod, the bottom end of the movable bead bottom connecting rod is fixedly connected with a test tube close insertion disc connecting rod, the side of the test tube close insertion disc connecting rod away from the movable bead bottom connecting rod is fixedly connected with a test tube close insertion disc, the top of the test tube close insertion disc is aligned with the bottom of the test tube insertion disc, and the inner wall of the test tube insertion disc and the test tube close insertion disc is movably connected with a separation test tube.

[0018] As a further scheme of the present application: the angle adjusting mechanism comprises an adjusting mechanism sliding block, the outer wall of the adjusting mechanism sliding block is slidably connected with the inner wall of the sliding groove disc sliding groove, the bottom of the adjusting mechanism sliding block is rotatably connected with the outer wall of the rotating rod away from the lifting shaft, the outer side of the adjusting mechanism sliding block is rotatably connected with a hinged block, and the side of the hinged block away from the adjusting mechanism sliding block is fixedly connected with a test tube fixing tube.

[0019] As a further scheme of the present application: the middle part of the inner wall of the hinge block is fixedly connected with a fixed tube sliding groove rod on the front and back sides, respectively, the top of the two fixed tube sliding groove rods is slidably connected with an L-shaped sliding block on the left and right sides, the inner side of the left and right groups of L-shaped sliding blocks is fixedly connected with a contraction and expansion plate, the inner side of the two contraction and expansion plates is fixedly connected with a semicircular clamping plate, the outer side of the two contraction and expansion plates is fixedly connected with an outer contraction and expansion plate at the bottom, the inner side of the two outer contraction and expansion plates is rotatably connected with a bottom tray rotating rod at the bottom, the inner side of the two bottom tray rotating rods is fixedly connected with a spring, the side of the two bottom tray rotating rods away from the outer contraction and expansion plate is rotatably connected with a bottom tray rotating rod hinge block.

[0020] As a further scheme of the present application: the inner side of the two outer contraction and expansion plates away from the two bottom tray rotating rods is fixedly connected with a bidirectional hydraulic push rod, the top of the bottom tray rotating rod hinge block is fixedly connected with a bottom tray, the inner side of the two semicircular clamping plates is made of rubber.

[0021] As a further scheme of the present application: the plurality of test tube temperature monitoring mechanisms each include a monitoring mechanism main plate, the inner side of the plurality of monitoring mechanism main plates is fixedly connected with the plurality of adjustment mechanism sliding blocks at the top, the left and right sides of the inner side of the plurality of monitoring mechanism main plates is fixedly connected with an L-shaped sliding groove plate, the side of the plurality of groups of L-shaped sliding groove plates away from the monitoring mechanism main plate is provided with an L-shaped sliding groove plate sliding groove, the middle part of the inner bottom of the plurality of monitoring mechanism main plates is fixedly connected with a hydraulic push rod connecting block, the inner wall of the plurality of hydraulic push rod connecting blocks is fixedly connected with a hydraulic push rod.

[0022] As a further scheme of the present application: the outer wall of the plurality of monitoring mechanism main plates is fixedly connected with a vertical connecting rod on the left and right sides, the inner side of the plurality of groups of vertical connecting rods is fixedly connected with a concave plate at the top, the outer wall of the plurality of hydraulic push rod connecting blocks is provided with a concave plate side sliding groove on the left and right sides, the inner wall of the plurality of concave plates is slidably connected with a semicircular temperature measuring plate connecting rod, the left and right sides of the plurality of semicircular temperature measuring plate connecting rods close to the monitoring mechanism main plate is fixedly connected with a push-pull rod, the outer side of the plurality of groups of push-pull rods is extended to the outer wall of the concave plate on the left and right sides through the concave plate side sliding groove, the side of the plurality of semicircular temperature measuring plate connecting rods away from the monitoring mechanism main plate is extended to the outer wall of the concave plate and is fixedly connected with a semicircular temperature measuring plate, the side of the plurality of groups of L-shaped sliding groove plates away from the monitoring mechanism main plate is slidably connected with a rectangular connecting frame sliding block, the inner side of the plurality of groups of rectangular connecting frame sliding blocks is fixedly connected with a rectangular connecting frame, the inner wall of the plurality of rectangular connecting frames is fixedly connected with a push-pull vertical rod, the top of the plurality of push-pull vertical rods is fixedly connected with a push-pull rotating rod connecting plate, the bottom of the plurality of rectangular connecting frames is fixedly connected with the top end of the hydraulic push rod, the outer wall of the plurality of push-pull rotating rod connecting plates is rotatably connected with a push-pull rotating rod on the left and right sides, the inner side of the plurality of groups of push-pull rotating rods away from the push-pull rotating rod connecting plate is rotatably connected with the outer side of the plurality of groups of push-pull rods, respectively.

[0023] The present application has the advantages of:

[0024] 1、The separation test tube rack, the separation test tube connecting disc and the angle adjusting mechanism can be flexibly adjusted according to different experimental requirements, specifically, after the separation test tube is inserted into the test tube insertion disc, the outer expansion plate and the semicircular clamping plate are moved inward by starting the bidirectional hydraulic push rod, and the bottom of the test tube outer wall is adhered, when the test tube angle is changed, the bottom tray rotating rod is rotated, the bottom tray is lifted to stabilize the test tube, after the lead screw motor is started, the transmission shaft drives the lifting shaft to move up and down, and then the adjusting mechanism slider slides in the sliding groove, the push-pull of the test tube fixed tube is realized, since the test tube is adhered to the insertion disc, the expansion and contraction movement of the adjusting mechanism slider can adjust the test tube inclination angle, this design ensures the efficiency and safety of stem cell extraction, and the operation is convenient, the mistakes are reduced, the parts of the device work cooperatively, the extraction purity and efficiency are improved, and if the device needs to be adjusted in all directions, the motor can be started to realize it.

[0025] 2、The test tube temperature monitoring mechanism is provided, so that the temperature control strategy can be adjusted in time by the operator, the stem cells are separated and extracted in the best low-temperature environment, the extraction efficiency and cell activity are maximized, specifically, after the hydraulic push rod is started, the semicircular temperature measuring plate is adhered to the test tube outer wall, and the temperature change in the test tube is monitored in real time. The temperature data is accurately transmitted to the monitoring mainboard and displayed on the screen in real time. DETAILED DESCRIPTION

[0026] Figure 1 is a schematic diagram of the main body of the present application;

[0027] Figure 2 is a schematic diagram of the main body of the present application;

[0028] Figure 3 is a schematic diagram of the main body of the present application;

[0029] Figure 4 is a schematic diagram of the main body of the present application;

[0030] Figure 5 is a schematic diagram of the separation test tube rack of the present application;

[0031] Figure 6 is a schematic diagram of the separation test tube rack of the present application;

[0032] Figure 7 is a schematic diagram of the separation test tube connecting disc of the present application;

[0033] Figure 8 is a schematic diagram of the angle adjusting mechanism and the test tube temperature monitoring mechanism of the present application;

[0034] Figure 9 The perspective separation structure schematic diagram of the angle adjusting mechanism of the present application;

[0035] Figure 10 The perspective separation structure schematic diagram of the test tube temperature monitoring mechanism of the present application.

[0036] In the figure: 1, extraction separation device main body; 2, separation test tube rack; 21, bottom connecting disc; 22, track; 23, motor; 24, transmission disc; 25, connecting column; 26, connecting column sliding groove; 27, sliding groove disc; 28, sliding groove disc sliding groove; 29, screw rod motor; 210, screw rod; 211, transmission shaft; 212, connecting block; 213, lifting shaft; 214, rotating rod; 3, separation test tube connecting disc; 31, separation test tube connecting disc main body; 32, connecting rod; 33, movable bead connecting block; 34, test tube insertion disc; 35, movable bead; 36, test tube insertion disc connecting rod; 37, test tube insertion disc connecting rod; 38, test tube insertion disc; 39, separation test tube; 4, angle adjusting mechanism; 41, adjusting mechanism sliding block; 42, hinged block; 43, test tube fixing tube; 44, fixing tube sliding groove rod; 45, L-shaped sliding block; 46, contraction and expansion plate; 47, outer contraction and expansion plate; 48, bidirectional hydraulic push rod; 49, bottom tray rotating rod; 410, spring; 411, bottom tray rotating rod hinged block; 412, bottom tray; 413, semicircular clamping plate; 5, test tube temperature monitoring mechanism; 51, monitoring mechanism main plate; 52, L-shaped sliding groove plate; 53, L-shaped sliding groove plate sliding groove; 54, hydraulic push rod connecting block; 55, hydraulic push rod; 56, vertical connecting rod; 57, concave plate; 58, concave plate side sliding groove; 59, semicircular temperature measuring plate connecting rod; 510, push-pull rod; 511, semicircular temperature measuring plate; 512, rectangular connecting frame sliding block; 513, rectangular connecting frame; 514, push-pull vertical rod; 515, push-pull rotating rod connecting plate; 516, push-pull rotating rod. DETAILED DESCRIPTION

[0037] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the scope of protection of the present application.

[0038] As Figures 1-4As shown, the present application provides a stem cell extraction device, comprising an extraction separation device body 1, a separation test tube rack 2 is movably connected to the inner wall of the extraction separation device body 1, a separation test tube connecting disc 3 is fixedly connected to the top of the outer wall of the separation test tube rack 2, an angle adjusting mechanism 4 is movably connected to the bottom of the outer wall of the separation test tube rack 2 in an annular array, and a test tube temperature monitoring mechanism 5 is fixedly connected to the inner side of each of the plurality of angle adjusting mechanisms 4.

[0039] The present application also provides a stem cell extraction method, comprising the following steps:

[0040] Step one: Put the cell liquid that needs to be extracted and separated into a specially designed centrifugal tube, and ensure that the liquid volume is moderate to avoid overflow;

[0041] Step two: Start the extraction separation device body 1, set appropriate speed and time, and make the cells stratified and precipitated;

[0042] Step three: Carefully suck the supernatant with a sterile pipette to avoid disturbing the precipitate layer;

[0043] Step four: Transfer the precipitated cells to a new centrifugal tube, add an appropriate amount of washing liquid, and centrifuge again;

[0044] Step five: Repeat the washing and centrifugation steps until the cells are pure;

[0045] Step six: Collect the pure stem cells and perform subsequent culture or experimental operation.

[0046] As Figures 2-9As shown, the separation test tube rack 2 comprises a bottom connecting disc 21 rotatably connected to the middle of the inner wall of the extraction and separation device main body 1, the outer wall of the bottom connecting disc 21 is sleeved with a track 22, the inner wall of the side away from the bottom connecting disc 21 of the track 22 is sleeved with a transmission disc 24, the bottom of the transmission disc 24 is fixedly connected with a motor 23, the bottom of the transmission disc 24 is fixedly connected with the output end of the motor 23, the bottom of the motor 23 is fixedly connected to the inner wall of the bottom of the extraction and separation device main body 1 away from the bottom connecting disc 21, the top of the bottom connecting disc 21 is fixedly connected with a connecting column 25, the outer wall of the connecting column 25 is annularly arrayed with connecting column sliding grooves 26, the outer wall of the connecting column 25 away from the plurality of connecting column sliding grooves 26 is fixedly connected with a sliding groove disc 27, the top of the sliding groove disc 27 is annularly arrayed with sliding groove disc sliding grooves 28 penetrating through to the bottom, the inner wall of the bottom connecting disc 21 is fixedly connected with a lead screw motor 29, the output end of the lead screw motor 29 extends to the inner wall of the connecting column 25 and is fixedly connected with a lead screw 210, the outer wall of the lead screw 210 is threadedly connected with a transmission shaft 211, the outer wall of the transmission shaft 211 is annularly arrayed with connecting blocks 212, the outer sides of the plurality of connecting blocks 212 extend to the outer wall of the connecting column 25 through the connecting column sliding grooves 26 and are fixedly connected with lifting shafts 213, the outer wall of the lifting shaft 213 is annularly arrayed with rotating rods 214, the separation test tube connecting disc 3 comprises a separation test tube connecting disc main body 31, the outer wall of the separation test tube connecting disc main body 31 is annularly arrayed with connecting rods 32, the side away from the separation test tube connecting disc main body 31 of the plurality of connecting rods 32 is fixedly connected with test tube insertion discs 34, the outer wall of the side close to the test tube insertion disc 34 of the plurality of connecting rods 32 is fixedly connected with movable bead connecting blocks 33, the inner wall of the bottom of the plurality of movable bead connecting blocks 33 is rotatably connected with movable beads 35, the outer wall of the bottom of the plurality of movable beads 35 is fixedly connected with movable bead bottom connecting rods 36, the bottom ends of the plurality of movable bead bottom connecting rods 36 are fixedly connected with test tube close insertion disc connecting rods 37, the side away from the movable bead bottom connecting rod 36 of the plurality of test tube close insertion disc connecting rods 37 is fixedly connected with test tube close insertion discs 38, the top of the plurality of test tube close insertion discs 38 is aligned with the bottom of the test tube insertion disc 34, the inner walls of the plurality of test tube insertion discs 34 and test tube close insertion discs 38 are movably connected with separation test tubes 39, the plurality of angle adjusting mechanisms 4 comprise adjusting mechanism sliding blocks 41, the outer walls of the plurality of adjusting mechanism sliding blocks 41 are respectively slidably connected in the inner walls of the plurality of sliding groove disc sliding grooves 28 opened by the sliding groove disc 27, the bottoms of the plurality of adjusting mechanism sliding blocks 41 are respectively rotatably connected to the outer walls of the sides of the plurality of rotating rods 214 away from the lifting shaft 213, the outer sides of the plurality of adjusting mechanism sliding blocks 41 are rotatably connected with hinged blocks 42, the sides away from the adjusting mechanism sliding blocks 41 of the plurality of hinged blocks 42 are fixedly connected with test tube fixing tubes 43, the front and back sides of the middle of the inner wall of the hinged block 42 are respectively fixedly connected with fixing tube sliding groove rods 44, the left and right sides of the top of the two fixing tube sliding groove rods 44 are slidably connected with L-shaped sliding blocks 45, the inner sides of the two groups of L-shaped sliding blocks 45 are fixedly connected with expansion plates 46,The inner side of the two collecting and expanding plates 46 is fixedly connected with a semicircular clamping plate 413, the bottom of the outer side of the two collecting and expanding plates 46 is fixedly connected with an outer collecting and expanding plate 47, the inner bottom of the two outer collecting and expanding plates 47 is rotatably connected with a bottom tray rotating rod 49, the inner side of the two bottom tray rotating rods 49 is fixedly connected with a spring 410, the side, away from the outer collecting and expanding plate 47, of the two bottom tray rotating rods 49 is rotatably connected with a bottom tray rotating rod hinged block 411, the inner side, away from the two bottom tray rotating rods 49, of the two outer collecting and expanding plates 47 is fixedly connected with a bidirectional hydraulic push rod 48, the top of the bottom tray rotating rod hinged block 411 is fixedly connected with a bottom tray 412, and the inner side of the two semicircular clamping plates 413 is made of rubber,

[0047] When the separation test tube 39 is poured into the stem cell material, the separation test tube 39 is then inserted into the inner wall of the test tube insertion disc 34 and the test tube fitting disc 38, the outer wall bottom of the separation test tube 39 is inside the two semicircular clamps 413 on the inner wall of the test tube fixing tube 43, when the angle of the separation test tube 39 needs to be changed during the separation and extraction, at this time, the bidirectional hydraulic push rod 48 on the inner wall of the test tube fixing tube 43 is started, the bidirectional hydraulic push rod 48 is started to pull the two outer expansion plates 47 on both sides to move inward, the two outer expansion plates 47 move inward to pull the two expansion plates 46 and the semicircular clamps 413 on the inner side of the expansion plate 46 to move inward and fit the outer wall bottom of the separation test tube 39, at the same time, when the two outer expansion plates 47 move inward, the two bottom tray rotating rods 49 are rotated to change the angle and then push the bottom tray 412 upward to support the bottom of the separation test tube 39 through the bottom tray rotating rod hinge block 411, so that the separation test tube 39 is stable when the angle is changed, then start the lead screw motor 29, after the lead screw motor 29 is started, the lead screw 210 rotating rod is rotated, the lead screw 210 is rotated to drive the transmission shaft 211 to move up and down on the outer wall of the lead screw 210, when the transmission shaft 211 moves up and down on the outer wall of the lead screw 210, the lifting shaft 213 is driven to move up and down on the outer wall of the connecting column 25 through the outer wall of the multiple connecting blocks 212, thereby driving the rotating rod 214 to pull the adjustment mechanism slider 41 to slide in the slide groove 28 on the inner wall of the slide groove disc 27, thereby the multiple rotating rods 214 drive the multiple adjustment mechanism sliders 41 to expand and contract in the multiple slide groove discs 28 on the inner wall of the slide groove disc 27, the multiple adjustment mechanism sliders 41 expand and contract to push and pull the test tube fixing tube 43, at this time, since the two semicircular clamps 413 on the inner wall of the test tube fixing tube 43 fit the outer wall of the separation test tube 39, and the test tube fitting disc 38 that holds and fits the separation test tube 39 is fixed with the test tube fitting disc connecting rod 37, the test tube fitting disc connecting rod 37 is fixed with the movable ball 35 through the movable ball bottom connecting rod 36, the movable ball 35 is rotatably connected to the inner wall bottom of the movable ball connecting block 33, thereby, when the adjustment mechanism slider 41 expands and contracts, the angle of the separation test tube 39 is adjusted, in this way, the angle adjustment of the separation test tube 39 is not only accurate but also stable, which ensures the efficiency and safety of the stem cell extraction process, in addition, the design of the entire device fully considers the convenience of operation, so that the operator can easily master the adjustment skill and reduce the possibility of operation error, during the adjustment process, the components of the device work cooperatively to ensure the stability of the separation test tube and the accuracy of the inclination angle, thereby improving the purity and efficiency of stem cell extraction, when the separation test tube rack 2, the separation test tube connecting disc 3, the angle adjustment mechanism 4, the test tube temperature monitoring mechanism 5 need to be rotated as a whole, only need to start the motor 23, the motor 23 is started to drive the whole through the transmission disc 24, the track 22 transmission bottom connecting disc 21 and the top to rotate, thereby realizing the overall adjustment of the entire device.

[0048] As Figure 10 shown, the plurality of test tube temperature monitoring mechanisms 5 each include a monitoring mechanism main plate 51, the outer sides of the plurality of monitoring mechanism main plates 51 are fixedly connected to the inner side top portions of the plurality of adjusting mechanism sliders 41, the inner sides of the plurality of monitoring mechanism main plates 51 are each fixedly connected with an L-shaped sliding groove plate 52, the sides of the plurality of L-shaped sliding groove plates 52 away from the monitoring mechanism main plates 51 are each provided with an L-shaped sliding groove plate sliding groove 53, the middle portions of the inner bottom portions of the plurality of monitoring mechanism main plates 51 are each fixedly connected with a hydraulic push rod connecting block 54, the inner walls of the plurality of hydraulic push rod connecting blocks 54 are each fixedly connected with a hydraulic push rod 55, the outer walls of the plurality of monitoring mechanism main plates 51 are each fixedly connected with a vertical connecting rod 56, the inner side top portions of the plurality of vertical connecting rods 56 are each fixedly connected with a concave plate 57, the outer walls of the plurality of hydraulic push rod connecting blocks 54 are each provided with a concave plate side sliding groove 58, the inner walls of the plurality of concave plates 57 are each slidingly connected with a semicircular temperature measuring plate connecting rod 59, the sides of the plurality of semicircular temperature measuring plate connecting rods 59 close to the monitoring mechanism main plates 51 are each fixedly connected with a push-pull rod 510, the outer sides of the plurality of push-pull rods 510 are each extended to the outer walls of the concave plates 57 through the concave plate side sliding grooves 58, the sides of the plurality of semicircular temperature measuring plate connecting rods 59 away from the monitoring mechanism main plates 51 are each extended to the outer walls of the concave plates 57 and are each fixedly connected with a semicircular temperature measuring plate 511, the sides of the plurality of L-shaped sliding groove plates 52 away from the monitoring mechanism main plates 51 are each slidingly connected with a rectangular connecting frame sliding block 512, the inner sides of the plurality of rectangular connecting frame sliding blocks 512 are each fixedly connected with a rectangular connecting frame 513, the inner walls of the plurality of rectangular connecting frames 513 are each fixedly connected with a push-pull vertical rod 514, the top portions of the plurality of push-pull vertical rods 514 are each fixedly connected with a push-pull rotating rod connecting plate 515, the bottom portions of the plurality of rectangular connecting frames 513 are each fixedly connected to the top ends of the hydraulic push rods 55, the outer wall sides of the plurality of push-pull rotating rod connecting plates 515 are each rotationally connected with a push-pull rotating rod 516, the inner sides of the plurality of push-pull rotating rods 516 away from the push-pull rotating rod connecting plates 515 are each rotationally connected to the outer sides of the plurality of push-pull rods 510;

[0049] The most suitable temperature for stem cell isolation and extraction is below 4 degrees. In this low temperature environment, the metabolic activity of cells is significantly reduced, effectively reducing the risk of stem cell damage and death during extraction. When monitoring the temperature during isolation and extraction, first start the hydraulic push rod 55. The hydraulic push rod 55 is activated to push the rectangular connecting frame 513, which drives the push-pull vertical rod 514 and the push-pull rotating rod connecting plate 515 to move towards the top. The push-pull rotating rod connecting plate 515 moves towards the top, thereby rotating through the push-pull rotating rod 516, which in turn pushes the push-pull rod 510, driving the semicircular temperature plate connecting rod 59 to slide on the inner wall of the concave plate 57. The semicircular temperature plate connecting rod 59 slides, thereby pushing the semicircular temperature plate 511 to adhere to the outer wall of the isolation test tube 39, ensuring that the temperature plate is in close contact with the outer wall of the test tube. Real-time monitoring of temperature changes in the test tube. Through this series of mechanical linkage, the temperature data is accurately transmitted to the monitoring mechanism mainboard 51 and displayed in real time on the display screen, allowing the operator to adjust the temperature control strategy in a timely manner, ensuring that the stem cells are isolated and extracted in the best low temperature environment, maximizing extraction efficiency and cell activity.

[0050] The working principle of the present application is as follows: after the stem cell material is poured into the separation test tube 39, the separation test tube 39 is inserted into the inner wall of the test tube insertion disc 34 and the test tube fitting disc 38, the outer wall bottom of the separation test tube 39 is inside the two semicircular clamps 413 on the inner wall of the test tube fixing tube 43, when the angle of the separation test tube 39 needs to be changed during the separation and extraction, at this time, the bidirectional hydraulic push rod 48 on the inner wall of the test tube fixing tube 43 is started, the bidirectional hydraulic push rod 48 is started to pull the two outer expansion plates 47 on both sides to move inward, the two outer expansion plates 47 move inward to pull the two expansion plates 46 and the semicircular clamps 413 on the inner side of the expansion plates 46 to move inward and fit the outer wall bottom of the separation test tube 39, at the same time, when the two outer expansion plates 47 move inward, the two bottom tray rotating rods 49 are rotated to change the angle and then push the bottom tray 412 upward to hold the bottom of the separation test tube 39 through the bottom tray rotating rod hinge block 411, so that the separation test tube 39 is stable when the angle is changed, then the lead screw motor 29 is started, after the lead screw motor 29 is started, the lead screw 210 rotating rod is rotated, the lead screw 210 is rotated to drive the transmission shaft 211 to move up and down on the outer wall of the lead screw 210, when the transmission shaft 211 moves up and down on the outer wall of the lead screw 210, the lifting shaft 213 is driven to move up and down on the outer wall of the connecting column 25 through the outer wall of the plurality of connecting blocks 212, so as to drive the rotating rod 214 to pull the adjustment mechanism slider 41 to slide in the slide groove 28 on the inner wall of the slide groove disc 27, so that the plurality of rotating rods 214 drive the plurality of adjustment mechanism sliders 41 to expand in the plurality of slide groove discs 28 on the inner wall of the slide groove disc 27, the plurality of adjustment mechanism sliders 41 expand to push and pull the test tube fixing tube 43, at this time, the two semicircular clamps 413 on the inner wall of the test tube fixing tube 43 fit the outer wall of the separation test tube 39, and the test tube fitting disc 38 holding the separation test tube 39 is fixed with the test tube fitting disc connecting rod 37, the test tube fitting disc connecting rod 37 is fixed with the movable ball 35 through the movable ball bottom connecting rod 36, the movable ball 35 is rotatably connected to the inner wall bottom of the movable ball connecting block 33, so that when the adjustment mechanism slider 41 expands, the angle of the separation test tube 39 is adjusted, in this way, the angle of the separation test tube 39 is not only accurate but also stable, which ensures the efficiency and safety of the stem cell extraction process, in addition, the design of the whole device fully considers the convenience of operation, so that the operator can easily master the adjustment skill and reduce the possibility of operation error, during the adjustment process, the components of the device work cooperatively to ensure the stability of the separation test tube and the accuracy of the inclination angle, thereby improving the purity and efficiency of the stem cell extraction, when the separation test tube rack 2, the separation test tube connecting disc 3, the angle adjustment mechanism 4, and the test tube temperature monitoring mechanism 5 need to be rotated as a whole, only the motor 23 is started, the motor 23 is started to drive the transmission disc 24, the crawler 22 transmission bottom connecting disc 21 and the whole on the top thereof to rotate,Thus, the whole device can be adjusted comprehensively.

[0051] The most suitable temperature for stem cell isolation and extraction is below 4 degrees. In this low temperature environment, the metabolic activity of the cells is significantly reduced, effectively reducing the risk of stem cell damage and death during the extraction process. When the temperature needs to be monitored during isolation and extraction, first start the hydraulic push rod 55, which pushes the rectangular connecting frame 513, drives the push-pull vertical rod 514 and the push-pull rotating rod connecting plate 515 to move to the top, the push-pull rotating rod connecting plate 515 moves to the top, thereby rotating through the push-pull rotating rod 516, and then pushing the push-pull rod 510 drives the semicircular temperature plate connecting rod 59 to slide on the inner wall of the concave plate 57, the semicircular temperature plate connecting rod 59 slides, thereby pushing the semicircular temperature plate 511 to adhere to the outer wall of the separation test tube 39, ensuring that the temperature plate is in close contact with the outer wall of the test tube, and the temperature change in the test tube is monitored in real time. Through this series of mechanical linkage, the temperature data is accurately transmitted to the monitoring mechanism mainboard 51, and is displayed in real time on the display screen, so that the operator can adjust the temperature control strategy in time, and ensure that the stem cells are isolated and extracted in the best low temperature environment, maximizing the extraction efficiency and cell activity.

[0052] The above shows and describes the basic principles and main features of the present application and the advantages of the present application. Those skilled in the art should understand that the present application is not limited to the above-mentioned embodiments, and the above-mentioned embodiments and descriptions in the specification are only to illustrate the principles of the present application. Without departing from the spirit and scope of the present application, various changes and improvements can be made to the present application, and these changes and improvements all fall within the scope of the claimed present application. The scope of protection of the present application is defined by the appended claims and their equivalents.

Claims

1. A stem cell extraction method, characterized by, The stem cell extraction method applied to the stem cell extraction device comprises the following steps: Step one: Put the cell liquid to be extracted and separated into a specially designed centrifugal tube, ensure the liquid volume is moderate, and avoid overflow; Step two: Start the extraction and separation device main body (1), set appropriate speed and time, and make the cells stratified and precipitated; Step three: Carefully suck the supernatant with a sterile pipette, avoiding disturbing the precipitate layer; Step four: Transfer the precipitated cells to a new centrifugal tube, add an appropriate amount of washing liquid, and centrifuge again; Step five: Repeat the washing and centrifugation steps until the cells are pure; Step six: Collect the pure stem cells for subsequent culture or experimental operation.

2. A stem cell extraction device, characterized by: The extraction and separation device main body (1) is provided with a separation test tube placing rack (2) movably connected to the inner wall of the extraction and separation device main body (1), a separation test tube connecting disc (3) fixedly connected to the top outer wall of the separation test tube placing rack (2), and an angle adjusting mechanism (4) movably connected to the bottom outer wall of the separation test tube placing rack (2) in an annular array. The inner side of each of the plurality of angle adjusting mechanisms (4) is fixedly connected with a test tube temperature monitoring mechanism (5). The separation test tube placing rack (2) comprises a bottom connecting disc (21) rotatably connected to the middle inner wall of the extraction and separation device main body (1) at the bottom of the bottom connecting disc (21). The outer wall of the bottom connecting disc (21) is sleeved with a track (22). The inner wall of the side of the track (22) away from the bottom connecting disc (21) is sleeved with a transmission disc (24). The bottom of the transmission disc (24) is fixedly connected with a motor (23). The bottom of the transmission disc (24) is fixedly connected to the output end of the motor (23). The bottom of the motor (23) is fixedly connected to the inner wall bottom of the extraction and separation device main body (1) on the side away from the bottom connecting disc (21).

3. The stem cell extraction device of claim 2, wherein: The top of the bottom connecting disc (21) is fixedly connected with a connecting column (25). The outer wall of the connecting column (25) is annularly arrayed with connecting column sliding grooves (26). The outer wall of the connecting column (25) on the side away from the plurality of connecting column sliding grooves (26) is fixedly connected with a sliding groove disc (27). The top of the sliding groove disc (27) is annularly arrayed with sliding groove disc sliding grooves (28) penetrating through to the bottom.

4. The stem cell extraction device of claim 3, wherein: The inner wall of the bottom connecting disc (21) is fixedly connected with a lead screw motor (29). The output end of the lead screw motor (29) extends to the inner wall of the connecting column (25) and is fixedly connected with a lead screw (210). The outer wall of the lead screw (210) is threadedly connected with a transmission shaft (211). The outer wall of the transmission shaft (211) is annularly arrayed with connecting blocks (212). The outer side of each of the plurality of connecting blocks (212) extends to the outer wall of the connecting column (25) through the connecting column sliding grooves (26) and is fixedly connected with a lifting shaft (213). The outer wall of the lifting shaft (213) is annularly arrayed with rotating rods (214).

5. The stem cell extraction device of claim 1, wherein: The separation test tube connecting plate (3) comprises a separation test tube connecting plate body (31), the outer wall annular array of the separation test tube connecting plate body (31) is fixedly connected with a connecting rod (32), a plurality of connecting rods (32) are fixedly connected with test tube insertion discs (34) away from the side of the separation test tube connecting plate body (31), the outer wall of the side of a plurality of connecting rods (32) close to the test tube insertion disc (34) is fixedly connected with a live bead connecting block (33), the inner wall of the bottom of a plurality of live bead connecting blocks (33) is rotatably connected with a live bead (35), the outer wall bottom of a plurality of live beads (35) is fixedly connected with a live bead bottom connecting rod (36), the bottom end of a plurality of live bead bottom connecting rods (36) is fixedly connected with a test tube close insertion disc connecting rod (37), the side of a plurality of test tube close insertion disc connecting rods (37) away from the live bead bottom connecting rod (36) is fixedly connected with a test tube close insertion disc (38), the top of a plurality of test tube close insertion discs (38) is aligned with the bottom of the test tube insertion disc (34), and the inner walls of a plurality of test tube insertion discs (34) and test tube close insertion discs (38) are movably connected with separation test tubes (39).

6. The stem cell extraction device of claim 1, wherein: A plurality of angle adjusting mechanisms (4) comprise an adjusting mechanism sliding block (41), the outer walls of a plurality of adjusting mechanism sliding blocks (41) are respectively slidably connected in the inner walls of a plurality of sliding groove sliding grooves (28) of the sliding groove disc (27), the bottoms of a plurality of adjusting mechanism sliding blocks (41) are respectively rotatably connected to the outer walls of the sides of a plurality of rotating rods (214) away from the lifting shaft (213), the outer sides of a plurality of adjusting mechanism sliding blocks (41) are rotatably connected with a hinged block (42), and the sides of a plurality of hinged blocks (42) away from the adjusting mechanism sliding block (41) are fixedly connected with a test tube fixing pipe (43).

7. The stem cell extraction device of claim 6, wherein: The front and back sides of the middle part of the inner wall of the hinged block (42) are respectively fixedly connected with a fixed pipe sliding groove rod (44), the left and right sides of the top of the two fixed pipe sliding groove rods (44) are slidably connected with an L-shaped sliding block (45), the inner sides of the left and right groups of L-shaped sliding blocks (45) are fixedly connected with a contraction and expansion plate (46), the inner sides of the two contraction and expansion plates (46) are fixedly connected with a semicircular clamping plate (413), the bottom outer sides of the two contraction and expansion plates (46) are fixedly connected with an outer contraction and expansion plate (47), the bottoms of the inner sides of the two outer contraction and expansion plates (47) are rotatably connected with a bottom tray rotating rod (49), the inner sides of the two bottom tray rotating rods (49) are fixedly connected with a spring (410), and the sides of the two bottom tray rotating rods (49) away from the outer contraction and expansion plate (47) are rotatably connected with a bottom tray rotating rod hinged block (411).

8. The stem cell extraction device of claim 7, wherein: The sides of the inner sides of the two outer contraction and expansion plates (47) away from the two bottom tray rotating rods (49) are fixedly connected with a bidirectional hydraulic push rod (48), the top of the bottom tray rotating rod hinged block (411) is fixedly connected with a bottom tray (412), and the inner sides of the two semicircular clamping plates (413) are made of rubber.

9. The stem cell extraction device of claim 1, wherein: The plurality of test tube temperature monitoring mechanisms (5) each include a monitoring mechanism main plate (51), the outer sides of the plurality of monitoring mechanism main plates (51) are fixedly connected to the inner side top portions of the plurality of adjusting mechanism sliders (41), the inner sides of the plurality of monitoring mechanism main plates (51) are fixedly connected with L-shaped sliding groove plates (52) on both sides, the sides of the plurality of groups of L-shaped sliding groove plates (52) away from the monitoring mechanism main plates (51) are each provided with an L-shaped sliding groove plate sliding groove (53), the middle portions of the inner bottom sides of the plurality of monitoring mechanism main plates (51) are each fixedly connected with a hydraulic push rod connecting block (54), and the inner walls of the plurality of hydraulic push rod connecting blocks (54) are each fixedly connected with a hydraulic push rod (55).

10. The stem cell extraction device of claim 9, wherein: The outer walls of the plurality of monitoring mechanism main plates (51) are each fixedly connected with a vertical connecting rod (56), the inner side top portions of the plurality of groups of vertical connecting rods (56) are each fixedly connected with a concave plate (57), the outer walls of the plurality of hydraulic push rod connecting blocks (54) are each provided with a concave plate side sliding groove (58), the inner walls of the plurality of concave plates (57) are each slidingly connected with a semicircular temperature measuring plate connecting rod (59), the sides of the plurality of semicircular temperature measuring plate connecting rods (59) close to the monitoring mechanism main plates (51) are each fixedly connected with a push-pull rod (510), the outer sides of the plurality of groups of push-pull rods (510) are each extended to the outer walls of the concave plates (57) through the concave plate side sliding grooves (58), the sides of the plurality of semicircular temperature measuring plate connecting rods (59) away from the monitoring mechanism main plates (51) are each extended to the outer walls of the concave plates (57) and are each fixedly connected with a semicircular temperature measuring plate (511), the sides of the plurality of groups of L-shaped sliding groove plates (52) away from the monitoring mechanism main plates (51) are each slidingly connected with a rectangular connecting frame sliding block (512), the inner sides of the plurality of groups of rectangular connecting frame sliding blocks (512) are each fixedly connected with a rectangular connecting frame (513), the inner walls of the plurality of rectangular connecting frames (513) are each fixedly connected with a push-pull vertical rod (514), the top portions of the plurality of push-pull vertical rods (514) are each fixedly connected with a push-pull rotating rod connecting plate (515), the bottoms of the plurality of rectangular connecting frames (513) are each fixedly connected to the top ends of the hydraulic push rods (55), the outer walls of the plurality of push-pull rotating rod connecting plates (515) are each rotationally connected with a push-pull rotating rod (516), and the inner sides of the plurality of groups of push-pull rotating rods (516) away from the push-pull rotating rod connecting plates (515) are each rotationally connected to the outer sides of the plurality of groups of push-pull rods (510).

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