Method and device for chelating trace elements after enzymolysis of sargassum

Abstract

The invention relates to the technical field of gulfweed processing, in particular to a method and device for chelating trace elements after gulfweed enzymolysis, and the method comprises the following steps: firstly preparing gulfweed zymolyte, performing solid-phase chelation on the gulfweed zymolyte, zinc sulfate, copper sulfate and manganese sulfate under the action of a sodium hydroxide aqueous solution, and performing solid-phase chelation on the gulfweed zymolyte, zinc sulfate, copper sulfate and manganese sulfate; uniformly dispersing the obtained pre-chelation product in deionized water, adding a ferrous sulfate aqueous solution under the protection of nitrogen, uniformly stirring and mixing, adjusting the pH value, carrying out a stirring chelation reaction in a pulsed electric field, centrifuging to take a supernatant, adding mesoporous silica microspheres, and carrying out ultrasonic oscillation treatment and spray drying. The device comprises a supporting part, a displacement part is installed in the supporting part, guide vertical rods are symmetrically installed in the supporting part, a driving motor is fixed to the center of the top end of the supporting part, and the displacement part comprises a synchronizing device and a bearing device; the active ingredients of the sargassum subjected to enzymolysis treatment are chelated with trace elements, so that the feed conversion rate is increased, and the growth of animals is promoted.

Description

Technical Field

[0001] This invention relates to the field of Sargassum processing technology, specifically to a method and apparatus for chelating trace elements after enzymatic hydrolysis of Sargassum. Background Technology

[0002] Sargassum is not a single species of seaweed, but a collective term for hundreds of species of seaweed in the Sargassum family. They belong to the Phaeophyta phylum and are a very important group of marine organisms with unique ecological significance. Sargassum is an important raw material for extracting sodium alginate, which is a very important feed additive.

[0003] Trace elements play a vital role in maintaining normal physiological functions and health in animals. They are essential components and activators of enzymes, directly participating in the synthesis of animal tissues. Therefore, adding trace elements during feed processing is essential. Current feed products typically use inorganic salts to add trace elements, but animal absorption rates are very low. The vast majority of these trace elements are excreted by animals, polluting water bodies and farmland, leading to serious resource waste and environmental pollution problems.

[0004] After enzymatic hydrolysis, the chelation of active ingredients and trace elements in Sargassum not only enables the reuse of seaweed resources but also promotes the development of the feed industry. However, existing Sargassum chelation devices for chelating trace elements after enzymatic hydrolysis lack internal components for intermittent additive delivery, requiring timed additions. Furthermore, the built-in stirring blades can only agitate at the bottom of the reaction chamber, preventing the intermittent addition of additives and efficient synchronous stirring during operation. Therefore, an improved device is needed to address these issues. Summary of the Invention

[0005] The purpose of this invention is to provide a method and apparatus for chelating trace elements after enzymatic hydrolysis of Sargassum, so as to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution: A method for chelating trace elements after enzymatic hydrolysis of Sargassum, characterized by comprising the following steps: S1. Select fresh Sargassum, wash away impurities and salt, dry and grind into powder, sieve and mature at high temperature; add the matured Sargassum powder to phosphate buffer solution and soak, adjust pH to obtain Sargassum solution; then add compound enzyme to Sargassum solution for enzymatic hydrolysis, after enzymatic hydrolysis, enzyme inactivation and freeze drying to obtain Sargassum hydrolysate. S2. Then, the Sargassum hydrolysate from step S1 is chelated with zinc sulfate, copper sulfate, and manganese sulfate in a sodium hydroxide aqueous solution to obtain a pre-chelated product. S3. The prechelated product is then evenly dispersed in deionized water. Ferrous sulfate aqueous solution is added under nitrogen protection, stirred and mixed, pH is adjusted, and the chelation reaction is carried out under pulsed electric field conditions. The supernatant is collected by centrifugation, and finally mesoporous silica microspheres are added. The mixture is then subjected to ultrasonic oscillation and spray drying to obtain the final product.

[0007] Preferably, the complex enzyme in step S1 is composed of cellulase, pectinase, papain and alginate lyase mixed in a mass ratio of 2:2:1:1.

[0008] A device for chelating trace elements after enzymatic hydrolysis of Sargassum includes a support component, a displacement component installed inside the support component, guide rods symmetrically fixedly installed inside the support component, and a drive motor fixedly installed at the top center of the support component. The displacement component includes a synchronization device and a bearing device. The synchronization device is fixedly installed inside the bearing device. The synchronization device includes a shaped bracket, a first gear, a first bevel gear, a second bevel gear, and a second gear. The first gear is rotatably mounted on the side end of the shaped bracket, the first bevel gear is fixedly mounted on the side end of the first gear away from the shaped bracket, and the second bevel gear is rotatably mounted on the bottom end of the shaped bracket away from the first gear, and the second gear is fixedly mounted at the bottom center of the second bevel gear.

[0009] Preferably, the bearing device includes a contact top frame, an extension bracket, a third gear, a first blade, an extension arm, a second blade, a limiting sleeve, a support base plate, a rotating plate, and a gear ring. The limiting sleeve is fixedly installed on the outer ring of the support base plate, the rotating plate is rotatably installed on the outer ring of the limiting sleeve, the second blade is fixedly installed on the outer ring of the rotating plate and is arranged in a ring. The gear ring is fixedly installed at the top of the rotating plate, the extension brackets are symmetrically fixedly installed at the top of the support base plate, the third gear is rotatably installed between the two extension brackets, the extension arm is fixedly installed on both sides of the third gear and is arranged in a ring, the first blade is fixedly installed on the top of the side end of the extension arm opposite to the third gear, and the contact top frame is fixedly installed at the top of the extension bracket.

[0010] Preferably, the supporting component includes a reaction chamber, a lead screw, a stirring blade, a first rack, and an observation window. The observation window is fixedly installed on one side of the top of the reaction chamber, the alignment device is fixedly installed on the other side of the top of the reaction chamber, the lead screw is rotatably installed inside the reaction chamber, the stirring blade is fixedly installed on the bottom of the outer ring of the lead screw, and the stirring blade is arranged in a ring. The first rack is fixedly installed on both sides inside the reaction chamber.

[0011] Preferably, the alignment device includes an accumulation cavity, a delivery pipe, a return spring, a displacement base plate, a sealing plug, a second rack, a fourth gear, a third rack, an extension vertical frame, a positioning top frame, and a support base. The delivery pipe is fixedly installed at the bottom end of the accumulation cavity. The support base is symmetrically fixedly installed at the bottom side of the accumulation cavity. The positioning top frame is fixedly installed at the bottom of the outer ring of the delivery pipe. The sealing plug is slidably inserted into the bottom end of the delivery pipe. The displacement base plate is fixedly installed at the bottom end of the sealing plug. The return spring is symmetrically fixedly installed between the displacement base plate and the positioning top frame. The extension vertical frame is fixedly installed on the side of the positioning top frame away from the accumulation cavity. The third rack is slidably inserted into the side of the extension vertical frame away from the accumulation cavity. The fourth gear is rotatably installed at the bottom end of the extension vertical frame. The second rack is fixedly installed at the bottom end of the displacement base plate.

[0012] Preferably, the bottom end of the support base is connected to the top end of the reaction chamber away from the observation window, the center thread of the support base plate is threaded onto the outer ring of the lead screw, the third gear meshes with the first gear, and the first bevel gear meshes with the second bevel gear.

[0013] Preferably, the second gear meshes with the gear ring, the support base plate is slidably sleeved on the outer ring of the guide rod, the support base plate has a threaded hole in the center of its interior, and the support base plate has symmetrically formed circular holes inside its interior.

[0014] Preferably, the transmission ratio between the third gear and the first gear is 1:10, the transmission ratio between the gear ring and the second gear is 1:10, the contact top frame is vertically aligned with the third rack, and the two sides of the extended vertical frame are provided with slots.

[0015] Preferably, the third rack and the displacement base plate are both equipped with locking keys at the end near the extension vertical frame. The interior of the accumulation cavity is hollow and is interconnected with the conveying pipe. The third rack and the second rack are respectively meshed with the fourth gear, and the third gear is perpendicularly aligned with the first rack.

[0016] Compared with the prior art, the beneficial effects of the present invention are as follows: I. In this invention, the drive motor, when in operation, can move the support base plate up and down via the lead screw, allowing the third gear to move along the surface of the first rack, thereby enabling the first blade to rotate. When the third gear rotates, it can drive the first gear to rotate, and the second gear can drive the gear ring to rotate, allowing the second blade to rotate horizontally. By having the first and second blades rotate in opposite directions horizontally and vertically, the liquid inside the reaction chamber can be efficiently agitated, improving the mixing uniformity of the liquid and completing the work of efficient synchronous agitation of the liquid.

[0017] Second, when the supporting base moves upward, the contact top frame can be moved to contact the third rack. The third rack can then drive the displacement base plate to move downward through the fourth gear, allowing the additive liquid inside the storage cavity to be discharged into the interior of the reaction cavity through the delivery pipe. Furthermore, when the supporting base moves downward, the reset spring can drive the displacement base plate to move upward and reset, allowing the sealing plug to seal the interior of the delivery pipe, preventing excessive downward discharge of the additive liquid inside the storage cavity, thus completing the intermittent additive delivery process. Attached Figure Description

[0018] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a partial cross-sectional view of the main body of the present invention; Figure 2 This is a three-dimensional structural diagram of the main body from a frontal perspective in this invention; Figure 3 This is a three-dimensional structural diagram of the displacement component from a frontal view in this invention; Figure 4 This is a three-dimensional structural diagram of the synchronization device from a frontal perspective in this invention; Figure 5 This is a three-dimensional structural diagram of the bearing device from the front view in this invention; Figure 6 This is a partial cross-sectional schematic diagram of the support component in this invention; Figure 7 This is a three-dimensional structural diagram of the bottom end of the supporting component in this invention; Figure 8 This is a three-dimensional structural diagram of the alignment device from the front view in this invention.

[0020] In the diagram: 1-Displacement component, 2-Support component, 3-Drive motor, 4-Synchronization device, 5-Bearing device, 6-Irregularly shaped bracket, 7-First gear, 8-First bevel gear, 9-Second bevel gear, 10-Second gear, 11-Contact top frame, 12-Extension bracket, 13-Third gear, 14-First blade, 15-Extension arm, 16-Second blade, 17-Restriction sleeve, 18-Support base plate, 19-Rotating plate, 20-Gear ring, 21-Alignment device 22-Reaction chamber, 23-Screw rod, 24-Stirring blade, 25-First rack, 26-Observation window, 27-Accumulation chamber, 28-Transfer pipe, 29-Reset spring, 30-Displacement base plate, 31-Sealing rubber plug, 32-Second rack, 33-Fourth gear, 34-Third rack, 35-Extension vertical frame, 36-Positioning top frame, 37-Support base, 38-Guide vertical rod, 39-Nitrogen injection valve, 40-Ultrasonic oscillating rod, 41-Pulse electrode rod. Detailed Implementation

[0021] To enable those skilled in the art to better understand the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present application, and not all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative effort should fall within the scope of protection of the present application.

[0022] It should be noted that the terms "first," "second," etc., in the specification, claims, and accompanying drawings of this application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be interchanged where appropriate for the embodiments of this application described herein. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion; for example, a process, method, system, product, or apparatus that comprises a series of steps or units is not necessarily limited to those steps or units explicitly listed, but may include other steps or units not explicitly listed or inherent to such processes, methods, products, or apparatus.

[0023] The invention will be further described below with reference to the accompanying drawings. Example 1

[0024] A method for chelating trace elements after enzymatic hydrolysis of Sargassum, characterized by comprising the following steps: S1. Select fresh Sargassum, wash to remove impurities and salt, dry and grind into powder, sieve and mature at high temperature; add the matured Sargassum powder to phosphate buffer solution and soak, adjust pH to obtain Sargassum solution; then add compound enzyme to Sargassum solution for enzymatic hydrolysis, after enzymatic hydrolysis, enzyme inactivation and freeze drying to obtain Sargassum hydrolysate; wherein, the compound enzyme is composed of cellulase, pectinase, papain and alginate lyase mixed in a mass ratio of 2:2:1:1.

[0025] S2. Then, the Sargassum hydrolysate from step S1 is chelated with zinc sulfate, copper sulfate, and manganese sulfate in a sodium hydroxide aqueous solution to obtain a pre-chelated product. S3. The prechelated product is then evenly dispersed in deionized water. Ferrous sulfate aqueous solution is added under nitrogen protection, stirred and mixed, pH is adjusted, and the chelation reaction is carried out under pulsed electric field conditions. The supernatant is collected by centrifugation, and finally mesoporous silica microspheres are added. The mixture is then subjected to ultrasonic oscillation and spray drying to obtain the final product.

[0026] Example 1: The enzymatically hydrolyzed Sargassum polysaccharides and amino acids can act as chelating ligands, forming stable chelates with zinc, copper, manganese, and iron metal ions. This protects trace elements from binding with other substances in the digestive tract, significantly improving animal absorption rates. Simultaneously, the alginic acid and alginic acid in Sargassum have a binding effect, stabilizing feed form and preventing dispersion in water, while also promoting intestinal digestion and absorption of nutrients. Chelated trace elements improve feed conversion rates and promote animal growth by optimizing metabolic pathways. Example 2

[0027] Please see Figure 1 , Figure 2 , Figure 3 , Figure 4 An embodiment of the present invention includes a support component 2, a displacement component 1 installed inside the support component 2, guide vertical rods 38 symmetrically fixedly installed inside the support component 2, and a drive motor 3 fixedly installed at the top center of the support component 2. The displacement component 1 includes a synchronization device 4 and a bearing device 5. The synchronization device 4 is fixedly installed inside the bearing device 5. The synchronization device 4 includes a shaped bracket 6, a first gear 7, a first bevel gear 8, a second bevel gear 9, and a second gear 10. The first gear 7 is rotatably installed on the side end of the shaped bracket 6. The first bevel gear 8 is fixedly installed on the side end of the first gear 7 away from the shaped bracket 6. The second bevel gear 9 is rotatably installed on the bottom end of the shaped bracket 6 away from the first gear 7. The second gear 10 is fixedly installed at the bottom center of the second bevel gear 9.

[0028] like Figure 5The supporting device 5 includes a contact top frame 11, an extension bracket 12, a third gear 13, a first blade 14, an extension arm 15, a second blade 16, a limiting sleeve 17, a supporting base plate 18, a rotating plate 19, and a gear ring 20. The limiting sleeve 17 is fixedly installed on the outer ring of the supporting base plate 18, the rotating plate 19 is rotatably installed on the outer ring of the limiting sleeve 17, the second blade 16 is fixedly installed on the outer ring of the rotating plate 19 and is arranged in a ring, the gear ring 20 is fixedly installed on the top of the rotating plate 19, the extension bracket 12 is symmetrically fixedly installed on the top of the supporting base plate 18, the third gear 13 is rotatably installed between the two extension brackets 12, the extension arm 15 is fixedly installed on both sides of the third gear 13 and is arranged in a ring, the first blade 14 is fixedly installed on the top of the side end of the extension arm 15 away from the third gear 13, and the contact top frame 11 is fixedly installed on the top of the extension bracket 12. The first blade 14 and the second blade 16 can rotate in two directions, and the solution can be stirred from multiple directions.

[0029] like Figure 6 and Figure 7 The supporting component 2 includes a reaction chamber 22, a lead screw 23, a stirring blade 24, a first rack 25, and an observation window 26. The observation window 26 is fixedly installed on one side of the top of the reaction chamber 22, and the alignment device 21 is fixedly installed on the other side of the top of the reaction chamber 22. The lead screw 23 is rotatably installed inside the reaction chamber 22. The stirring blade 24 is fixedly installed at the bottom of the outer ring of the lead screw 23 and is arranged in a ring. The first rack 25 is fixedly installed on both sides inside the reaction chamber 22. The reaction chamber 22 can be sealed by the observation window 26. At the same time, a pulsed electric field is installed inside the reaction chamber 22 to assist in the chelation of the solution inside the reaction chamber 22.

[0030] like Figure 8The alignment device 21 includes an accumulation cavity 27, a conveying pipe 28, a return spring 29, a displacement base plate 30, a sealing rubber plug 31, a second rack 32, a fourth gear 33, a third rack 34, an extension vertical frame 35, a positioning top frame 36, and a support base 37. The conveying pipe 28 is fixedly installed at the bottom end of the accumulation cavity 27, the support base 37 is symmetrically fixedly installed at the bottom side of the accumulation cavity 27, the positioning top frame 36 is fixedly installed at the bottom of the outer ring of the conveying pipe 28, the sealing rubber plug 31 is slidably inserted into the bottom end of the conveying pipe 28, and the displacement base plate 30 is fixedly installed at the bottom end of the sealing rubber plug 31. The reset spring 29 is symmetrically fixed between the displacement base plate 30 and the positioning top frame 36. The extension vertical frame 35 is fixedly installed on the side end of the positioning top frame 36 away from the accumulation cavity 27. The third rack 34 is slidably inserted into the side end of the extension vertical frame 35 away from the accumulation cavity 27. The fourth gear 33 is rotatably installed at the bottom end of the extension vertical frame 35. The second rack 32 is fixedly installed at the bottom end of the displacement base plate 30. With the reset spring 29, the displacement base plate 30 can be moved upward and reset when the contact top frame 11 is not in contact with the third rack 34. The sealing rubber plug 31 can block the conveying pipe 28.

[0031] The bottom end of the support base 37 is connected to the top end of the reaction chamber 22 away from the observation window 26. The center thread of the support base plate 18 is threaded onto the outer ring of the lead screw 23. The third gear 13 meshes with the first gear 7, the first bevel gear 8 meshes with the second bevel gear 9, and the second gear 10 meshes with the gear ring 20. The support base plate 18 is slidably sleeved on the outer ring of the guide rod 38. A threaded hole is opened in the center of the support base plate 18, and circular holes are symmetrically opened inside the support base plate 18. The transmission between the third gear 13 and the first gear 7... The ratio is 1:10, the transmission ratio between the gear ring 20 and the second gear 10 is 1:10, the contact top frame 11 is vertically aligned with the third rack 34, the extension vertical frame 35 has slots on both sides, the third rack 34 and the displacement base plate 30 are both equipped with key on the end near the extension vertical frame 35, the interior of the accumulation cavity 27 is hollow, and the accumulation cavity 27 is interconnected with the conveying pipe 28, the third rack 34 and the second rack 32 are respectively meshed with the fourth gear 33, and the third gear 13 is vertically aligned with the first rack 25.

[0032] In this embodiment, the pre-chelated product and deionized water are first transported to the interior of the reaction chamber 22 through the observation window 26. Then, the prepared ferrous sulfate aqueous solution is placed into the accumulation chamber 27. When the return spring 29 is in its normal state, it can drive the sealing plug 31 to seal the delivery pipe 28, preventing the ferrous sulfate aqueous solution inside the accumulation chamber 27 from flowing downwards. Subsequently, the drive motor 3 can be turned on. The drive motor 3 is connected to the top of the lead screw 23, allowing the lead screw 23 to rotate. When the lead screw 23 rotates, it drives the stirring paddle 24 to rotate at the bottom of the interior of the reaction chamber 22, thus agitating the liquid. Furthermore, the outer ring of the lead screw 23 is threaded, and the support base plate 18 is threaded onto the lead screw 23. On the outer ring, the lead screw 23, when rotating, can drive the support base plate 18 to move upward. The support base plate 18 is slidably sleeved on the outer ring of the guide vertical rod 38, ensuring that the support base plate 18 moves vertically in a straight line. Simultaneously, when the support base plate 18 moves, it can drive the third gear 13 to move along the surface of the first rack 25. Thus, the third gear 13 can drive the extension arm 15 and the first blade 14 to rotate, allowing the first blade 14 to agitate the liquid inside the reaction chamber 22. At the same time, when the third gear 13 rotates, it can drive the first gear 7 to rotate, and the first gear 7 can drive the second bevel gear 9 and the second gear 10 to rotate via the first bevel gear 8. This allows the second gear 10 to drive the gear ring 20 to rotate. When the gear ring 20 rotates... The rotating plate 19 can be driven to rotate, so that the second blade 16 can rotate inside the reaction chamber 22, stirring the liquid inside the reaction chamber 22. Through the arrangement of the first blade 14 and the second blade 16, the liquid can be stirred from two directions, improving the uniformity of the stirring. Furthermore, as the supporting base plate 18 moves up and down, the first blade 14 and the second blade 16 can stir the liquid at different heights inside the reaction chamber 22. Subsequently, when the supporting base plate 18 moves upward to its limit position, it can drive the contact top frame 11 to move to contact the third rack 34, thereby squeezing the third rack 34 upward to its limit position. This allows the third rack 34 to drive the second rack 32 and the displacement base plate 30 downward through the fourth gear 33. At this time, the seal... The rubber stopper 31 can be pulled out from the inside of the delivery pipe 28 and communicates with the delivery pipe 28 through the accumulation chamber 27. The lower part of the delivery pipe 28 is located at the top of the inside of the reaction chamber 22, so that the ferrous sulfate aqueous solution inside the accumulation chamber 27 can be discharged into the inside of the reaction chamber 22 through the delivery pipe 28, which facilitates the mixing of the ferrous sulfate aqueous solution with the pre-chelation product and deionized water inside the reaction chamber 22. After the sealed rubber stopper 31 is separated from the delivery pipe 28 for a specified time, the delivery pipe 28 can deliver a certain amount of ferrous sulfate aqueous solution into the inside of the reaction chamber 22 in a short time. Subsequently, the drive motor 3 can drive the lead screw 23 to rotate in the opposite direction, so that the support plate 18 can move downward. At this time, the support plate 18 can drive the third gear 13 to move downward along the surface of the first rack 25.Thus, the first blade 14 and the second blade 16 can rotate in opposite directions, allowing the various solutions inside the reaction chamber 22 to be stirred again, improving the mixing efficiency of the various solutions. Furthermore, when the support base plate 18 moves downwards, it can cause the contact top frame 11 to disengage from the third rack 34. At this time, the return spring 29 will cause the displacement base plate 30 to move upwards to reset. When the displacement base plate 30 moves upwards, it can cause the second rack 32 to move upwards, thereby allowing the second rack 32 to drive the third rack 34 downwards to reset via the fourth gear 33, completing the reset of the third rack 34. While the second rack 32 is displaced, locking keys are installed on one end of the third rack 34 and the displacement base plate 30, and these keys are slidably inserted into the inside of both sides of the extension vertical frame 35. This ensures that the displacement base plate 30 and the third rack 34 move up and down in a straight line. In use, the screw 23 rotates in both directions, driving the support base plate 18 to move up and down. As the support base plate 18 moves, it drives the third gear 13 to move along the surface of the first rack 25. This allows the first blade 14 and the second blade 16 to rotate in multiple directions, efficiently agitating the solution inside the reaction chamber 22. Furthermore, the support base plate... 18 can drive the first blade 14 and the second blade 16 to move up and down, stirring in different areas of the solution. Simultaneously, when the support plate 18 moves upward to its limit position each time, the contact top frame 11 can squeeze the third rack 34 upward, causing the displacement base plate 30 to move downward, releasing the ferrous sulfate aqueous solution inside the delivery pipe 28, thus achieving the purpose of timed and quantitative addition of the ferrous sulfate aqueous solution. During use, nitrogen gas can be supplied into the reaction chamber 22 through the nitrogen injection valve 39, which can change the properties of the chelates inside the reaction chamber 22 and the performance of the final product. At the same time, nitrogen gas can... Air is removed from the top of reaction chamber 22 to create an inert atmosphere. Then, an ultrasonic oscillating rod 40 is placed inside reaction chamber 22, and the oscillating rod 40 is in contact with the solution inside reaction chamber 22. When the ultrasonic oscillating rod 40 is running, it can forcefully "push" the solution into the nanoscale pores of silica, making the silica microspheres uniformly dispersed and achieving physical adsorption and binding. Furthermore, charged ions move directionally under the electric field of pulsed electrode rod 41, reaching the chelation sites more quickly, releasing the active ingredients within the cells. This physical field technology enhances and accelerates the chelation reaction, completing the process.

[0033] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A method for chelating trace elements after enzymatic hydrolysis of Sargassum, characterized in that, Includes the following steps: S1. Select fresh Sargassum, wash away impurities and salt, dry and grind into powder, sieve and mature at high temperature; add the matured Sargassum powder to phosphate buffer solution and soak, adjust pH to obtain Sargassum solution; then add compound enzyme to Sargassum solution for enzymatic hydrolysis, after enzymatic hydrolysis, enzyme inactivation and freeze drying to obtain Sargassum hydrolysate. S2. Then, the Sargassum hydrolysate from step S1 is chelated with zinc sulfate, copper sulfate, and manganese sulfate in a sodium hydroxide aqueous solution to obtain a pre-chelated product. S3. The prechelated product is then evenly dispersed in deionized water. Ferrous sulfate aqueous solution is added under nitrogen protection, stirred and mixed, pH is adjusted, and the chelation reaction is carried out under pulsed electric field conditions. The supernatant is collected by centrifugation, and finally mesoporous silica microspheres are added. The mixture is then subjected to ultrasonic oscillation and spray drying to obtain the final product.

2. The method for chelating trace elements after enzymatic hydrolysis of Sargassum according to claim 1, characterized in that, In step S1, the complex enzyme is composed of cellulase, pectinase, papain and alginate lyase mixed in a mass ratio of 2:2:1:

1.

3. A device for chelating trace elements after enzymatic hydrolysis of Sargassum, employing the method for chelating trace elements after enzymatic hydrolysis of Sargassum as described in claim 2, characterized in that... The device includes a support component (2), a displacement component (1) is installed inside the support component (2), guide rods (38) are symmetrically fixed inside the support component (2), and a drive motor (3) is fixedly installed at the top center of the support component (2). The displacement component (1) includes a synchronization device (4) and a bearing device (5). The synchronization device (4) is fixedly installed inside the bearing device (5). The synchronization device (4) includes a shaped bracket (6), a first gear (7), a first bevel gear (8), a second bevel gear (9), and a second gear (10). The first gear (7) is rotatably installed on the side end of the shaped bracket (6). The first bevel gear (8) is fixedly installed on the side end of the first gear (7) away from the shaped bracket (6). The second bevel gear (9) is rotatably installed on the bottom end of the shaped bracket (6) away from the first gear (7). The second gear (10) is fixedly installed at the bottom center of the second bevel gear (9).

4. The device for chelating trace elements after enzymatic hydrolysis of Sargassum according to claim 3, characterized in that: The bearing device (5) includes a contact top frame (11), an extension bracket (12), a third gear (13), a first blade (14), an extension arm (15), a second blade (16), a limiting sleeve (17), a support base plate (18), a rotating plate (19), and a gear ring (20). The limiting sleeve (17) is fixedly installed on the outer ring of the support base plate (18), the rotating plate (19) is rotatably installed on the outer ring of the limiting sleeve (17), and the second blade (16) is fixedly installed on the outer ring of the rotating plate (19), and the second blade (16) is annularly divided. The toothed ring (20) is fixedly installed on the top of the rotating plate (19), the extension bracket (12) is symmetrically fixedly installed on the top of the support base plate (18), the third gear (13) is rotatably installed between the two extension brackets (12), the extension arm (15) is fixedly installed on both sides of the third gear (13) and the extension arm (15) is distributed in a ring, the first blade (14) is fixedly installed on the top of the side end of the extension arm (15) away from the third gear (13), and the contact top frame (11) is fixedly installed on the top of the extension bracket (12).

5. The device for chelating trace elements after enzymatic hydrolysis of Sargassum according to claim 4, characterized in that: The support component (2) includes a reaction chamber (22), a lead screw (23), a stirring blade (24), a first rack (25), and an observation window (26). The observation window (26) is fixedly installed on one side of the top of the reaction chamber (22), and the alignment device (21) is fixedly installed on the other side of the top of the reaction chamber (22). The lead screw (23) is rotatably installed inside the reaction chamber (22). The stirring blade (24) is fixedly installed at the bottom of the outer ring of the lead screw (23), and the stirring blade (24) is arranged in a ring. The first rack (25) is fixedly installed on both sides inside the reaction chamber (22).

6. The device for chelating trace elements after enzymatic hydrolysis of Sargassum according to claim 5, characterized in that: The alignment device (21) includes an accumulation cavity (27), a delivery pipe (28), a return spring (29), a displacement base plate (30), a sealing rubber plug (31), a second rack (32), a fourth gear (33), a third rack (34), an extension vertical frame (35), a positioning top frame (36), and a support base (37). The delivery pipe (28) is fixedly installed at the bottom end of the accumulation cavity (27), and the support base (37) is symmetrically fixedly installed at the bottom side of the accumulation cavity (27). The positioning top frame (36) is fixedly installed at the bottom of the outer ring of the delivery pipe (28), and the sealing rubber plug (31) is slidably inserted. The displacement base plate (30) is fixedly installed at the bottom of the sealing rubber plug (31) inside the conveying pipe (28). The reset spring (29) is symmetrically fixed between the displacement base plate (30) and the positioning top frame (36). The extension vertical frame (35) is fixedly installed on the side of the positioning top frame (36) away from the accumulation cavity (27). The third rack (34) is slidably inserted into the side of the extension vertical frame (35) away from the accumulation cavity (27). The fourth gear (33) is rotatably installed at the bottom of the extension vertical frame (35). The second rack (32) is fixedly installed at the bottom of the displacement base plate (30).

7. The device for chelating trace elements after enzymatic hydrolysis of Sargassum according to claim 6, characterized in that: The bottom end of the support base (37) is connected to the top end of the reaction chamber (22) away from the observation window (26). The center thread of the support base plate (18) is threaded onto the outer ring of the lead screw (23). The third gear (13) meshes with the first gear (7). The first bevel gear (8) meshes with the second bevel gear (9).

8. The device for chelating trace elements after enzymatic hydrolysis of Sargassum according to claim 7, characterized in that: The second gear (10) meshes with the gear ring (20), the support base plate (18) is slidably sleeved on the outer ring of the guide rod (38), the support base plate (18) has a threaded hole in the center of its interior, and the support base plate (18) has symmetrically opened round holes inside its interior.

9. The device for chelating trace elements after enzymatic hydrolysis of Sargassum according to claim 8, characterized in that: The transmission ratio between the third gear (13) and the first gear (7) is 1:10, the transmission ratio between the gear ring (20) and the second gear (10) is 1:10, the contact top frame (11) is vertically aligned with the third rack (34), and the extension vertical frame (35) has slots on both sides inside.

10. The device for chelating trace elements after enzymatic hydrolysis of Sargassum according to claim 9, characterized in that: Both the third rack (34) and the displacement base plate (30) are equipped with keyes at the end near the extension frame (35). The interior of the accumulation cavity (27) is hollow and is connected to the conveying pipe (28). The third rack (34) and the second rack (32) are respectively meshed with the fourth gear (33). The third gear (13) is vertically aligned with the first rack (25).

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