Full-automatic drop dyeing instrument capable of controlling drop dyeing

Abstract

The invention discloses a full-automatic drop dyeing instrument capable of controlling drop dyeing, which comprises a rack, a dye liquor supply module and a dye liquor reaction module are arranged in the rack, a liquid transfer module is arranged between the dye liquor supply module and the dye liquor reaction module, an output position of the liquid transfer module is provided with a water dropper with sucking and spraying functions, one side of the water dropper is provided with a cleaning module, and the other side of the water dropper is provided with a water pump. A dye liquor uniform spreading module is arranged in the middle of the dye liquor reaction module, a plurality of glass slides are arranged on the dye liquor supply module, and a bottom part of the dye liquor uniform spreading module moves horizontally by means of a driving structure on the outer side. According to the device, the dye liquor uniform spreading module is arranged, a plurality of horizontally-laid protrusions are formed on the periphery of the uniform pushing rotary seat, and the protrusions extend to the position below the bottom of the first pushing frame and can make contact with the dyeing table top, so that dye liquor uniform spreading is achieved in the moving process. Wherein each bulge can be uniformly paved once correspondingly to realize disposable use, and the bulge is rotated and displaced by virtue of external force every time the bulge is used so as not to be connected with the dyeing table top, so that the next bulge is alternately used to avoid cross contamination.

Description

Technical Field

[0001] This invention relates to the field of biological staining instruments, specifically a fully automated drip staining instrument with controllable drip staining. Background Technology

[0002] A staining apparatus is a precision instrument that uses a "dye solution dropwise addition" method to achieve automated staining. It is widely used in medical pathology, microbiological testing, and scientific research. Compared to traditional "immersion" or "spraying" methods, staining technology offers significant advantages such as precise control of dye solution volume, avoidance of cross-contamination, and improved staining consistency. However, cross-contamination remains a concern during instrument use. This is mainly due to the need for multiple transfers and tests, with all liquid samples being transferred and used through the same component. This can lead to residues on the component, causing cross-contamination in subsequent uses and affecting the accuracy of experimental data.

[0003] This case arose in order to resolve the aforementioned issues. Summary of the Invention

[0004] (a) Technical problems to be solved

[0005] To address the shortcomings of existing technologies, this invention provides a fully automatic drip dyeing instrument with controllable drip dyeing, which solves the problems mentioned in the background art.

[0006] (II) Technical Solution

[0007] To achieve the above objectives, the present invention provides the following technical solution: a fully automatic drip dyeing instrument with controllable drip dyeing, comprising a frame, wherein a dye liquor supply module and a dye liquor reaction module are provided within the frame, and a pipetting module is provided between the two. The output position of the pipetting module is provided with a dropper with suction and spray functions, wherein a cleaning module is assembled on one side of the dropper. A dye liquor spreading module is provided in the middle of the dye liquor reaction module. Multiple glass slides are placed on the dye liquor supply module. The bottom component of the dye liquor spreading module can move horizontally by means of an outer driving structure and can move to contact the glass slides.

[0008] As a preferred embodiment, the cleaning module further includes a storage bottle containing an independent cleaning bottle. A first water inlet and a second water inlet are respectively provided between the storage bottle and the cleaning bottle along the same axial direction. An external water supply pipe passes through the first and second water inlets from the storage bottle to the interior of the cleaning bottle. A conical water outlet groove is provided at the bottom of the storage bottle, and the cleaning bottle is supported on the inner wall of the storage bottle by a support block.

[0009] As a preferred embodiment, the height of the cleaning bottle is less than the height of the storage bottle, and its dimensions are adapted to the dimensions of the dropper.

[0010] As a preferred embodiment, the dye supply module further includes a rotatable dye supply frame, with multiple compartments evenly spaced circumferentially on the dye supply frame, and each compartment containing a supply bottle.

[0011] As a preferred embodiment, the dyeing solution reaction module further includes a support, on which a dyeing chamber is fixedly mounted. A second motor is provided below the dyeing chamber, and the output end of the second motor is connected upward to the dyeing chamber and equipped with a rotating platform. The rotating platform is independent of the dyeing chamber, and dyeing platforms are evenly distributed circumferentially on the rotating platform. Each dyeing platform is embedded with a glass slide.

[0012] As a preferred embodiment, the dye spreading module further includes a first pusher and a second pusher that are movably assembled with each other, and the two have a height difference with the dyeing table surface. The second pusher is a fixed part and is connected and fixed to a drive structure on one side. The first pusher is a movable assembly part and has a spreading rotating seat at its bottom, which is pivotally connected to the first pusher.

[0013] As a preferred embodiment, the outer periphery of the uniform pusher has a plurality of horizontally laid protrusions that extend to the bottom of the first pusher and can contact the dyeing table surface.

[0014] As a preferred embodiment, the side of the uniform pusher is coaxially connected with a small gear, wherein the upper and lower positions of the second pusher are respectively provided with a driving gear and a driven gear that mesh with each other. The driving gear is driven to rotate by a micro motor. The first pusher is provided with a mounting slot for accommodating the driving gear, the driven gear and the micro motor. After the first pusher and the second pusher are assembled, the driven gear meshes with the small gear.

[0015] As a preferred embodiment, the bottom surface of the first pusher is further attached with a layer of absorbent cotton, and the protrusion there is rotated to contact it.

[0016] As a preferred embodiment, the first pusher and the second pusher are further provided with magnets of different magnetic properties on their mating surfaces.

[0017] (III) Beneficial Effects

[0018] By adopting the above technical solution, the present invention has the following beneficial effects compared with the prior art:

[0019] A fully automatic drop dyeing instrument with controllable drop dyeing is provided, with a cleaning module installed on one side of the pipetting module. The cleaning module includes a storage bottle, which contains an independent cleaning bottle. A first water inlet and a second water inlet are respectively opened between the storage bottle and the cleaning bottle in the same axial direction. An external water supply pipe connects the storage bottle to the cleaning bottle to supply water. The height of the cleaning bottle is smaller than that of the storage bottle, so that the water can be replaced after each use of the dropper for cleaning, that is, the internal water can be completely renewed through continuous water supply.

[0020] A fully automatic drip dyeing instrument with controllable drip dyeing is provided, comprising a dye liquor spreading module. The outer periphery of the spreading rotating base has multiple horizontally arranged protrusions extending below the bottom of the first pusher frame and contacting the dyeing table surface, thereby achieving uniform dye liquor spreading during movement. Each protrusion can be used once for single-use spreading, and after each use, it is rotated and repositioned by external force to avoid contact with the dyeing table surface, allowing for alternating use of the next protrusion and preventing cross-contamination. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the internal structure of the present invention;

[0022] Figure 2 This is a schematic diagram of the pipetting module of the present invention;

[0023] Figure 3 This is a schematic diagram of the liquid storage bottle of the present invention;

[0024] Figure 4 This is a schematic cross-sectional view of the liquid storage bottle of the present invention;

[0025] Figure 5 This is a schematic diagram of the dye solution reaction module of the present invention;

[0026] Figure 6 This is a schematic diagram of the structure of the dye solution spreading module of the present invention;

[0027] Figure 7 This is a partial disassembly diagram of the dye solution spreading module of the present invention;

[0028] Figure 8 This is a schematic diagram of the other side of the disassembled dye solution spreading module of the present invention.

[0029] In the diagram: 1. Frame; 2. Dye liquor supply module; 02. Box compartment; 3. Supply bottle; 4. Storage bottle; 41. First water inlet; 42. Water outlet; 43. Cleaning bottle; 44. Second water inlet; 45. Support block; 5. Transfer module; 51. Bracket; 52. First screw; 53. First motor; 54. Drive wheel; 55. Belt; 56. Driven wheel; 57. Rotating rod; 6. Dropper; 7. Dye liquor reaction module; 71. Support. 72. Second motor; 73. Dyeing chamber; 74. Rotating platform; 75. Dyeing table; 76. Second screw; 77. Drive motor; 78. Dye liquor spreading module; 781. First pusher; 782. Second pusher; 783. Spreading rotary seat; 784. Pinion; 785. Drive gear; 786. Followed gear; 787. Placement groove; 788. Micro motor; 789. Protrusion; 8. Control module; 9. Cleaning water pump. Detailed Implementation

[0030] To make the objectives, technical solutions, and advantages of the present invention clearer, the present invention will be further described in detail below with reference to specific embodiments and accompanying drawings.

[0031] Cross-contamination in this application mainly occurs in two steps: first, a small amount of the test liquid is transferred through the same pipette when it is transferred to the reaction detection station; second, each slide or each sample area is individually dyed, but the test liquid needs to be evenly spread after it is transferred to facilitate subsequent operations. At this time, the components used for spreading will cause cross-contamination due to residue.

[0032] See appendix Figure 1-2 As shown, a fully automatic drip dyeing instrument with controllable drip dyeing includes a frame 1, a dye liquor supply module 2 and a dye liquor reaction module 7 are provided in the frame 1, and a liquid transfer module 5 is provided between the two for transferring the liquid supplied on the dye liquor supply module 2 to the dye liquor reaction module 7 for reaction and detection.

[0033] The dye supply module 2 includes a rotatable dye supply frame, with 24 independent compartments 02 evenly spaced around the circumference for inserting the matching shaped supply bottles 3, enabling quick assembly and disassembly.

[0034] The pipetting module 5 is composed of conventional components and is mainly used to move the dropper 6 along the X and Y axes. The pipetting module 5 includes a support 51, on which a first screw 52, ​​driven to rotate by a motor located at the bottom, is vertically mounted. A horizontal support block is fixed to the first screw 52 via a threaded shaft block. A first motor 53 is fixed to the rear of this horizontal support block. A driving wheel 54 and a driven wheel 56 are pivotally connected to both sides of the horizontal support block, and the two are wrapped by a taut belt 55. The driving wheel 54 is connected to the output end of the first motor 53, and an L-shaped rotating rod 57 is connected to the driven wheel 56. The horizontal outer end of the rotating rod 57 is connected to the dropper 6 (a conventional piston-type pipetting dropper 6 with suction and spray functions can be used).

[0035] Dropper 6 is prone to leaving residue after liquid transfer, therefore it needs to be cleaned after each transfer. (See attached image) Figure 3-4 As shown, a cleaning module is installed on one side of the pipetting module 5. This cleaning module includes a storage bottle 4, which houses a separate cleaning bottle 43. A first water inlet 41 and a second water inlet 44, both aligned along the same axis, are respectively provided between the storage bottle 4 and the cleaning bottle 43. An external water supply pipe connects the storage bottle to the cleaning bottle 43 for water supply. The height of the cleaning bottle 43 is less than the height of the storage bottle 4, allowing the water to be replaced after each use of the dropper 6.

[0036] Specifically, a conical water outlet groove 42 is provided at the bottom of the storage bottle 4. The cleaning bottle 43 is supported on the inner wall of the storage bottle 4 by a support block 45. Each time the water is changed, water is continuously supplied through the water supply pipe, flowing out from the top while rinsing the inner wall of the cleaning pipe, and finally flowing out from the water storage groove at the bottom of the storage bottle 4. It should be noted that the diameter of the cleaning bottle 43 does not need to be too large (it only needs to be suitable for the size of the drip tip 6), so not too much water is wasted each time. After cleaning, a dryer can be installed on the rear side of the drip tip 6 for quick drying.

[0037] As attached Figure 5 As shown, the dyeing reaction module 7 includes a support 71, on which a dyeing chamber 73 is fixedly mounted. A second motor 72 is arranged below the dyeing chamber 73. The output end of the second motor 72 is connected upward to the dyeing chamber 73 and is equipped with a rotating platform 74. The rotating platform 74 is independent of the dyeing chamber 73, and dyeing platforms are evenly distributed circumferentially on the rotating platform 74. Each dyeing platform can be embedded with a glass slide for drop dyeing.

[0038] Under normal conditions, the center of the rotating platform 74 is also equipped with a dye liquor spreading module 78, which moves horizontally through a drive structure on one side. The drive component is a drive motor 77 located outside the dyeing chamber 73. It drives the second screw 76 located above through a belt pulley 55 and a belt 55, causing the second screw 76 to rotate. The second screw 76 is equipped with a shaft block connected to the dye liquor spreading module 78, which in turn drives it to move horizontally.

[0039] The dye spreading module 78 can be a blowing type, but the amount of dye liquid on the slide after the reaction is small, making it difficult to control the spreading effect. Therefore, this application improves the conventional blowing type to form a mechanical contact spreading.

[0040] See the appendix for details. Figure 6-8 As shown, the dye liquor spreading module 78 includes a first pusher 781 and a second pusher 782 that are movably assembled with each other. The two pushers have a height difference from the dyeing table surface 75. The second pusher 782 serves as a fixed part, connected and fixed to a drive structure on one side, and acts as a horizontal pushing part. The first pusher 781 serves as an assembly part, with a spreading rotating seat 783 at its bottom, which is pivotally connected to the first pusher 781. The outer periphery of the spreading rotating seat 783 has multiple horizontally laid protrusions 789 that extend below the bottom of the first pusher 781 and can contact the dyeing table surface 75, thereby achieving dye liquor spreading during movement.

[0041] Furthermore, each protrusion 789 can be evenly spread once for single use. Each time it is used, it can be rotated and moved by external force to avoid contact with the dyeing table 75, so as to achieve alternating use with the next protrusion 789 and avoid cross-contamination.

[0042] The structure of the externally driven rotating part is as follows: a small gear 784 is coaxially connected to the side of the uniform pusher 783. The second pusher 782 has a driving gear 785 and a driven gear 786 meshing with each other at its upper and lower positions, respectively. The driving gear 785 is driven to rotate by a micro motor 788. The corresponding first pusher 781 has a mounting slot 787 adapted to accommodate the driving gear 785, the driven gear 786, and the micro motor 788. After the first pusher 781 and the second pusher 782 are assembled, the driven gear 786 can mesh with the small gear 784, thereby achieving intermittent controlled rotation of the uniform pusher 783. To prevent residual liquid from dripping from the protrusion 789 after uniform pushing, a layer of absorbent cotton is attached to the bottom surface of the first pusher 781 to quickly absorb and dry the protrusion 789 upon contact.

[0043] The first pusher 781 and the second pusher 782 can be provided with magnets of opposite magnetic properties on their mating surfaces, so that they can be quickly assembled and easily removed for cleaning after one round of operation of the even pusher 783.

[0044] The dropper is added in 6 steps: silver nitrate → reducing agent → color enhancer (with temperature-controlled shaking between each step). A -5Pa negative pressure environment is constructed within the staining chamber 73, and the negative pressure system is activated upon heating to 60℃. The dye solution reacts on an independent glass slide within the staining chamber 73, and is tested after the reaction is complete by spreading it evenly. Conventional mechanisms such as the control module 8, temperature control module, and cleaning water supply module are not detailed here.

[0045] The entire process can be completed simply by placing the slide on the staining table 75, which improves work efficiency, simplifies manual steps, and also improves the quality of testing.

[0046] The specific embodiments described above further illustrate the purpose, technical solution, and beneficial effects of the present invention. It should be understood that the above descriptions are merely specific embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A fully automatic drip dyeing apparatus for controllable drip dyeing, comprising a frame, wherein a dye liquor supply module and a dye liquor reaction module are provided within the frame, and a pipetting module is provided between the two, characterized in that, The output position of the pipetting module is equipped with a dropper with suction and spray functions. A cleaning module is installed on one side of the dropper. A dye solution spreading module is provided in the middle of the dye solution reaction module. Multiple glass slides are placed on the dye solution supply module. The bottom part of the dye solution spreading module can move horizontally with the help of the outer drive structure and can move to the glass slide to contact it.

2. The fully automatic drip dyeing apparatus for controllable drip dyeing according to claim 1, characterized in that: The cleaning module includes a storage bottle containing an independent cleaning bottle. A first water inlet and a second water inlet are respectively provided between the storage bottle and the cleaning bottle along the same axis. An external water supply pipe passes through the first and second water inlets from the storage bottle to the cleaning bottle. A conical water outlet groove is provided at the bottom of the storage bottle. The cleaning bottle is supported on the inner wall of the storage bottle by a support block.

3. The fully automatic drip dyeing apparatus for controllable drip dyeing according to claim 2, characterized in that: The height of the cleaning bottle is less than that of the storage bottle, and its size is adapted to the size of the dropper.

4. The fully automatic drip dyeing apparatus for controllable drip dyeing according to claim 1, characterized in that: The dye supply module includes a rotatable dye supply frame, with multiple compartments evenly spaced circumferentially on the dye supply frame, and each compartment containing a supply bottle.

5. The fully automatic drip dyeing apparatus for controllable drip dyeing according to claim 1, characterized in that: The dyeing reaction module includes a support, on which a dyeing chamber is fixedly mounted. A second motor is located below the dyeing chamber, and the output end of the second motor is connected upward to the dyeing chamber and equipped with a rotating platform. The rotating platform is independent of the dyeing chamber, and dyeing platforms are evenly distributed circumferentially on the rotating platform. Each dyeing platform is embedded with a glass slide.

6. The fully automatic drip dyeing apparatus for controllable drip dyeing according to claim 5, characterized in that: The dye liquor spreading module includes a first pusher and a second pusher that are movably assembled with each other. The two pushers have a height difference with the dyeing table. The second pusher is a fixed part and is connected and fixed to a drive structure on one side. The first pusher is a movable part and has a spreading rotating seat at its bottom. The spreading rotating seat is pivotally connected to the first pusher.

7. The fully automatic drip dyeing apparatus for controllable drip dyeing according to claim 6, characterized in that: The outer periphery of the uniform pusher has multiple horizontally laid protrusions that extend to the bottom of the first pusher and can contact the dyeing table surface.

8. The fully automatic drip dyeing apparatus for controllable drip dyeing according to claim 7, characterized in that: The side of the uniform pusher is coaxially connected to a small gear. The upper and lower positions of the second pusher are respectively provided with a drive gear and a driven gear that mesh with each other. The drive gear is driven to rotate by a micro motor. The first pusher is provided with a mounting slot for accommodating the drive gear, the driven gear and the micro motor. After the first pusher and the second pusher are assembled, the driven gear meshes with the small gear.

9. The fully automatic drip dyeing apparatus for controllable drip dyeing according to claim 8, characterized in that: A layer of absorbent cotton is attached to the bottom surface of the first pusher, and the protrusion there is rotated to contact it.

10. The fully automatic drip dyeing apparatus for controllable drip dyeing according to claim 8, characterized in that: The first pusher and the second pusher have magnets with opposite magnetic properties on their mating surfaces.

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