Anti-radiation organic celery salt and preparation method thereof

Abstract

The invention discloses an anti-radiation organic celery salt and a preparation method thereof in the field of functional foods. The anti-radiation organic celery salt is prepared from the following raw materials in parts by weight: 25-55 parts of an organic celery extract; 150-200 parts of a functional mineral salt, 8-20 parts of a composite anti-radiation auxiliary agent and 6-12 parts of a functional auxiliary material; the composite anti-radiation additive is a plant extract combination and is selected from lycium barbarum polysaccharide, spirulina extract, sea-buckthorn extract and astragalus polysaccharide. Through a compound formula (the celery extract, the anti-radiation additive and the functional mineral salt) and a low-temperature embedding process, the anti-radiation effect (the maximum MDA reduction rate is 63.2%) and the stability (the 18-month activity retention rate is 90%) are remarkably improved, the product is superior to a single-component product or a traditional process product, the requirements of different scenes are met, and the application prospect is wide. The five types of formulas are respectively adapted to different scenes such as common people, high-radiation exposure people and low-salt demanders, are wider in applicability, have a seasoning function and can be directly used for cooking, and the problems that a traditional anti-radiation product needs to be taken independently and is poor in compliance are solved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of functional food, in particular to an anti-radiation organic celery salt and a preparation method thereof. BACKGROUND

[0002] With the wide application of radiation sources (such as medical radiation, electronic equipment, nuclear facilities), the potential harm of radiation to human health is increasingly prominent. Radiation induces the body to produce a large number of reactive oxygen species (ROS), leading to lipid peroxidation, DNA damage and immune function decline. Long-term exposure will increase the risk of cell mutation and chronic diseases. Therefore, the development of safe, efficient and easy-to-consume anti-radiation food has become the focus of industry research.

[0003] At present, the research and development of anti-radiation functional food has the following outstanding problems, which seriously restrict its application value:

[0004] 1. Single anti-radiation mechanism, lack of synergistic effect

[0005] a) Existing anti-radiation products mostly rely on single functional components and can only act through a single pathway:

[0006] b) Some products focus on antioxidant components (such as vitamin C, tea polyphenols), which can only scavenge a small amount of free radicals, but cannot improve radiation-induced immune suppression and DNA repair disorders;

[0007] c) Some products focus on immune regulation (such as polysaccharides), but have limited inhibitory effect on lipid peroxidation (such as MDA accumulation) caused by oxidative stress;

[0008] d) Single components have strong dose dependence, with weak effect at low dose and safety risk (such as liver and kidney metabolic burden) at high dose.

[0009] 2. Low retention rate of natural active components, rapid functional attenuation

[0010] a) Anti-radiation active components (such as flavonoids, polysaccharides) in natural plants (such as celery, medlar) are mostly heat-sensitive substances, and existing processing techniques have obvious defects:

[0011] b) High-temperature treatment (such as drying, cooking) leads to degradation of active components, for example, apigenin in celery has a sharp decrease in stability above 60℃, with a loss rate of more than 60% within 2 hours;

[0012] c) Lack of effective protection mechanism, active components are easily oxidized: exposed to air or light conditions, the retention rate of functional components is less than 50% within 3 months, which cannot meet the long-term storage demand;

[0013] d) Low release efficiency of active ingredients: Plant fiber wrapping leads to slow dissolution during digestion, with a bioavailability of less than 30%, making it difficult to achieve actual efficacy.

[0014] III. Limited application scenarios and poor user compliance

[0015] a) There are obvious limitations in the dosage form and functional design of existing anti-radiation products:

[0016] b) Most are special health products (such as tablets and oral liquids), which need to be taken separately and cannot be integrated into daily diet, making it easy to cause protection interruption due to "forgetting to take" during long-term use;

[0017] c) Split between function and seasoning properties: Most products have a single taste (such as bitter taste) and cannot be used as a seasoning, making it difficult to meet the needs of ordinary people for "synchronous protection during cooking";

[0018] d) Insufficient generalizability of formula: No differentiated solutions are designed for different radiation exposure intensities (such as low-dose long-term exposure and high-dose short-term exposure), with limited applicability.

[0019] Therefore, the skilled person in the art provides an anti-radiation organic celery salt and a preparation method thereof to solve the problems raised in the above background art. SUMMARY

[0020] The purpose of the present application is to provide an anti-radiation organic celery salt and a preparation method thereof to solve the problems raised in the above background art.

[0021] To achieve the above-mentioned purpose, the present application provides the following technical solutions:

[0022] An anti-radiation organic celery salt and a preparation method thereof, comprising the following raw materials in parts by weight:

[0023] 25-55 parts of organic celery extract, 150-200 parts of functional mineral salt, 8-20 parts of composite anti-radiation additive, and 6-12 parts of functional excipient; the composite anti-radiation additive is a combination of plant extracts selected from at least two of gynostemma pentaphyllum polysaccharide, spirulina extract, sea buckthorn extract, and astragalus polysaccharide.

[0024] As a further solution of the present application: the content of apigenin in the organic celery extract is ≥2.8 mg / g, the content of coumarin is ≥1.5 mg / g, and the total flavonoid content is ≥9.0 mg / g.

[0025] As a further solution of the present application: the functional mineral salt is a mixture of at least one of the following: sea salt (containing potassium ≥0.8%), rock salt (containing magnesium ≥0.5%), bamboo salt (containing zinc ≥0.03%), and low-sodium salt (sodium chloride ≤70%), with a total content of potassium, magnesium, and zinc in the mixture ≥3.5%.

[0026] As a further scheme of the present application: the combination of the composite anti-radiation adjuvant is:

[0027] Combination A: Wolfberry polysaccharide: Spirulina extract = 1:1-1.5;

[0028] Combination B: Sea buckthorn extract: Astragalus polysaccharide = 1:0.8-1.2;

[0029] Combination C: Wolfberry polysaccharide: Spirulina extract: Sea buckthorn extract = 1:1:0.5.

[0030] As a further scheme of the present application: the functional auxiliary material includes carrier auxiliary material, antioxidant auxiliary material and embedding auxiliary material; the carrier auxiliary material is malt dextrin or corn starch, the antioxidant auxiliary material is vitamin C or tea polyphenol, and the embedding auxiliary material is β-cyclodextrin or gum arabic, and the mass ratio of the three is 3:1:1-2.

[0031] As a further scheme of the present application: further comprising 0.5-2 parts of probiotic additives, the probiotic is Lactobacillus acidophilus or Bifidobacterium, and the viable bacterial count is ≥1×10 9 CFU / g.

[0032] A preparation method of an anti-radiation organic celery salt, comprising the following steps:

[0033] Step one, organic celery extract preparation: select organic planting celery stems and leaves, freeze dry at-35 to-25℃, crush, mix with 55%-65% ethanol at a solid-liquid ratio of 1:12-18, ultrasonic extract at 28-32℃, 250-350W for 40-50min, filter and concentrate to a solid content of ≥30%;

[0034] Step two, preparation of a composite anti-radiation adjuvant: mix plant extracts in the combination ratio of claim 4, stir at 300-500r / min for 15-20min;

[0035] Step three, mixing: mix the organic celery extract, functional mineral salt, composite anti-radiation adjuvant, and functional auxiliary material in proportion, and add 6%-9% deionized water to form a soft material;

[0036] Step four, granulation: pass the soft material through an 18-22 mesh sieve to granulate, and dry at 42-48℃ until the moisture content is ≤4%;

[0037] Step five, microcapsule embedding: mix the granules with the embedding auxiliary material at a ratio of 1:2.2-2.8, spray dry (inlet air temperature 165-175℃, outlet air temperature 72-78℃), and obtain the finished product.

[0038] As a further scheme of the present application: in the step one, the celery variety is "Xiqin No. 1", the moisture content after freeze-drying is ≤5%, and the crushing particle size is 100-120 meshes.

[0039] As a further scheme of the present application: in the step five, the microencapsulation embedding rate is ≥92%, the finished product is stored at 40℃ and relative humidity 75% for 18 months, and the apigenin retention rate is ≥88%.

[0040] Compared with the prior art, the present application has the beneficial effects that:

[0041] 1. The present application has a significant advantage in anti-radiation effect over single components through the multi-path combination of "apigenin antioxidant + polysaccharide immunomodulation + mineral supplement", and the MDA reduction rate is as high as 63.2%, solving the problem of single function of existing products.

[0042] 2. The present application uses low-temperature extraction + microencapsulation embedding process to make the apigenin retention rate reach more than 90%, and still maintain more than 88% activity after 18 months of storage, overcoming the defect that natural ingredients are easy to degrade.

[0043] 3. The five types of formulations of the present application are respectively adapted to different scenarios such as ordinary people, high radiation exposure, low salt demand, etc., and have wider applicability.

[0044] 4. The present application has a seasoning function and can be directly used for cooking, solving the problem of poor compliance of traditional anti-radiation products which need to be taken separately. DETAILED DESCRIPTION

[0045] The technical solutions in the embodiments of the present application will be described below in conjunction with the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0046] Example 1 (basic antioxidant type)

[0047] 1) Formulation (weight parts):

[0048] Organic celery extract 185 parts (apigenin 3.2 mg / g, total flavonoids ≥9.0 mg / g);

[0049] Functional mineral salt (sea salt: rock salt = 1:1) 30 parts;

[0050] Composite anti-radiation additive (combination A: wolfberry polysaccharide: spirulina extract = 1:1) 12 parts;

[0051] Functional excipient (malt dextrin: vitamin C: β-cyclodextrin = 3:1:1) 10 parts.

[0052] 2) Preparation method:

[0053] Step one, preparation of organic celery extract

[0054] Fresh organic celery stems and leaves were freeze-dried at -35°C (moisture ≤5%), ground and passed through a 100 mesh sieve;

[0055] 60% ethanol was added at a material-to-liquid ratio of 1:15, and 30°C, 300W ultrasonic extraction was performed for 45 minutes. The filtrate was concentrated to a solid content of ≥30%.

[0056] Step two, preparation of composite anti-radiation adjuvant

[0057] Gouqi polysaccharide and spirulina extract were mixed at a ratio of 1:1 and stirred at 300 r / min for 15 minutes until uniform.

[0058] Step three, mixing and granulation

[0059] All raw materials were mixed according to the formulation ratio, 7% deionized water was added to make soft material, and granulation was performed through an 18 mesh sieve;

[0060] Drying was performed at 45°C until the moisture content was ≤4%.

[0061] Step four, microencapsulation

[0062] The dried granules were mixed with β-cyclodextrin wall material at a ratio of 1:2.5, spray dried (inlet temperature 170°C, outlet temperature 75°C), and the finished product was obtained.

[0063] 3) Performance test:

[0064] Experimental design: Kunming mice were divided into a radiation control group (6Gy γ-ray irradiation), and an Example 1 group (dosed at 1g / kg·d by gavage, irradiated after 7 consecutive days)

[0065] In the radiation control group:

[0066]

[0067] In Example 1:

[0068]

[0069] Example 2 (immune-enhancing type)

[0070] 1) Formulation composition (by weight)

[0071] Organic celery extract: 195 parts (celeryin 3.0mg / g)

[0072] Functional mineral salt: 25 parts (bamboo salt: low sodium salt = 1:2, zinc 0.03%)

[0073] Composite anti-radiation adjuvant (combination B): 15 parts (sea buckthorn extract: astragalus polysaccharide = 1:1) Functional adjuvant: 12 parts (corn starch: tea polyphenol: gum arabic = 3:1:1.5) Probiotics: 1 part (Lactobacillus acidophilus, viable bacterial count ≥ 1 x 10 9 CFU / g)

[0074] 2) Key preparation steps

[0075] Same as example 1 (consistent extraction parameters), add probiotics during mixing, embedding rate 91.5%.

[0076] 3) Performance test results

[0077]

[0078]

[0079] Example 3 (all-round protection type)

[0080] 1) Formula composition (parts by weight)

[0081] Organic celery extract: 50 parts (apigenin 3.5 mg / g)

[0082] Functional mineral salt: 170 parts (sea salt: rock salt: bamboo salt = 1:1:1, total potassium, magnesium and zinc content 3.8%)

[0083] Composite anti-radiation adjuvant (combination C): 18 parts (gouqi polysaccharide: spirulina extract: sea buckthorn extract = 1:1:0.5) Functional adjuvant: 10 parts (malt dextrin: vitamin C: β-cyclodextrin = 3:1:2)

[0084] 2) Key preparation steps

[0085] Same extraction parameters as example 1, embedding rate 93.2%.

[0086] 3) Performance test results

[0087]

[0088]

[0089] Example 4 (low salt type)

[0090] 1) Formula composition (parts by weight)

[0091] Organic celery extract: 25 parts (apigenin 2.8 mg / g)

[0092] Functional mineral salt: 195 parts (low sodium salt: rock salt = 2:1, sodium chloride < 70%, potassium 1.2%) Composite anti-radiation aid (combination A): 8 parts (goji polysaccharide: spirulina extract = 1:1.5) Functional excipient: 10 parts (same as Example 1)

[0093] 2) Key preparation steps

[0094] Same as Example 1, embedding rate 90.8%.

[0095] 3) Performance test results

[0096]

[0097] Example 5 (high stability type)

[0098] 1) Formulation composition (parts by weight)

[0099] Organic celery extract: 40 parts (apigenin 3.2 mg / g)

[0100] Functional mineral salt: 180 parts (sea salt: bamboo salt = 1:1)

[0101] Composite anti-radiation aid (combination B): 15 parts (saccharum alfredii extract: astragalus polysaccharide = 1:0.8) Functional excipient: 12 parts (malt dextrin: tea polyphenol: gum arabic = 3:1:2)

[0102] 2) Key preparation steps

[0103] Same as Example 1, embedding rate 95% (increasing the ratio of wall material to enhance stability).

[0104] 3) Performance test results

[0105]

[0106]

[0107] Comparative Example 1 (lack of composite anti-radiation aid)

[0108] 1) Formulation: same as Example 3, remove composite anti-radiation aid.

[0109] 2) Performance test results:

[0110]

[0111]

[0112] Comparative Example 2 (traditional high-temperature extraction process)

[0113] 1) Formulation: same as Example 3, extraction process changed to 80°C water bath extraction for 2h.

[0114] 2) Performance test results:

[0115]

[0116] Comparative Example 3 (single component anti-radiation salt)

[0117] 1) Formulation: only functional mineral salt + vitamin C 5 parts (without celery extract and composite adjuvant).

[0118] 2) Performance test results:

[0119]

[0120]

[0121] The present application significantly improves the anti-radiation effect (MDA reduction rate of up to 63.2%) and stability (18-month activity retention rate of 90%) through the composite formulation (celery extract + anti-radiation adjuvant + functional mineral salt) and low-temperature embedding process, which is superior to single-component or traditional process products, and is suitable for different scene requirements. The five types of formulations are suitable for different scenes such as ordinary people, high radiation exposure, low salt demand, etc., have wider applicability, and also have seasoning function, can be directly used for cooking, solving the problem of poor compliance of traditional anti-radiation products which need to be taken separately.

[0122] The above description is only a preferred specific embodiment of the present application, but the protection scope of the present application is not limited thereto. Any person skilled in the art can make equivalent replacements or changes to the technical range disclosed in the present application according to the technical solution and inventive concept of the present application, which should be covered within the protection scope of the present application.

Claims

1. A radiation-resistant organic celery salt and its preparation method, characterized in that: It is made from the following raw materials in parts by weight: 25-55 parts organic celery extract; 150-200 parts functional mineral salt; 8-20 parts compound anti-radiation additive; 6-12 parts functional excipients; the compound anti-radiation additive is a combination of plant extracts, selected from at least two of the following: wolfberry polysaccharide, spirulina extract, sea buckthorn extract, and astragalus polysaccharide.

2. The anti-radiation organic celery salt according to claim 1, characterized in that: The organic celery extract contains apigenin content ≥2.8 mg / g, coumarin content ≥1.5 mg / g, and total flavonoid content ≥9.0 mg / g.

3. The anti-radiation organic celery salt according to claim 1, characterized in that: The functional mineral salt is a mixture of at least one of the following: sea salt (containing ≥0.8% potassium), rock salt (containing ≥0.5% magnesium), bamboo salt (containing ≥0.03% zinc), and low-sodium salt (containing ≤70% sodium chloride), wherein the total content of potassium, magnesium, and zinc in the mixture is ≥3.5%.

4. The anti-radiation organic celery salt according to claim 1, characterized in that: The combination method of the composite anti-radiation additive is as follows: Combination A: Lycium barbarum polysaccharide: Spirulina extract = 1:1-1.5; Combination B: Sea buckthorn extract: Astragalus polysaccharide = 1:0.8-1.2; Combination C: Lycium barbarum polysaccharide: Spirulina extract: Sea buckthorn extract = 1:1:0.

5.

5. The anti-radiation organic celery salt according to claim 1, characterized in that: The functional excipients include carrier excipients, antioxidant excipients, and encapsulation excipients; the carrier excipient is maltodextrin or corn starch, the antioxidant excipient is vitamin C or tea polyphenols, and the encapsulation excipient is β-cyclodextrin or gum arabic, with a mass ratio of 3:1:1-2.

6. The anti-radiation organic celery salt according to claim 1, characterized in that: It also includes 0.5-2 parts of probiotic additive, wherein the probiotics are Lactobacillus acidophilus or Bifidobacterium, with a live count ≥1×10⁻⁶. 9 CFU / g.

7. A method for preparing an anti-radiation organic celery salt, applied to an anti-radiation organic celery salt as described in any one of claims 1-6, characterized in that: Includes the following steps: Step 1: Preparation of organic celery extract: Select organically grown celery stems and leaves, freeze-dry them at -35 to -25℃, then pulverize them and mix them with 55%-65% ethanol at a material-to-liquid ratio of 1:12-18. Extract them with ultrasound at 28-32℃ and 250-350W for 40-50 minutes, and then filter and concentrate them until the solid content is ≥30%. Step 2: Preparation of composite anti-radiation additive: Mix plant extracts according to the combination ratio of claim 4, and stir at 300-500 r / min for 15-20 min; Step 3: Mixing: Mix organic celery extract, functional mineral salts, compound anti-radiation additives, and functional excipients in a certain proportion, and add 6%-9% deionized water to make a soft material; Step 4: Granulation: Soft material is granulated by passing through an 18-22 mesh sieve and dried at 42-48℃ until the moisture content is ≤4%; Step 5: Microcapsule encapsulation: Mix the granules and encapsulation excipients at a ratio of 1:2.2-2.8, and spray dry (inlet air 165-175℃, outlet air 72-78℃) to obtain the finished product.

8. The method for preparing an anti-radiation organic celery salt according to claim 7, characterized in that: In step one, the celery variety is "Xiqin No. 1", the moisture content after freeze-drying is ≤5%, and the particle size is 100-120 mesh.

9. The method for preparing an anti-radiation organic celery salt according to claim 7, characterized in that: In step five, the microcapsule encapsulation rate is ≥92%, and the finished product is stored at 40℃ and 75% relative humidity for 18 months with a apigenin retention rate of ≥88%.

10. The use of the anti-radiation organic celery salt according to any one of claims 1-6 in the preparation of anti-radiation seasonings or nutritional supplements.

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