Composition containing sophorolipid and magnolol as well as preparation method and application of composition

Abstract

The invention provides a composition containing sophorolipid and magnolol as well as a preparation method and application of the composition, and particularly relates to the technical field of daily chemical products. The composition is prepared from sophorolipid, magnolol and a cynanchum atratum extract according to the mass ratio of 1: (0.5-2.5): (0.8-1.2), the cynanchum atratum extract is prepared through an ultrasonic-assisted deep eutectic solvent extraction method, the obtained cynanchum atratum extract, sophorolipid and magnolol have a synergistic effect, and the antibacterial, anti-inflammatory, moisturizing and soothing effects of the composition can be improved.

Description

Technical Field

[0001] This invention belongs to the field of daily chemical product technology, specifically relating to a composition containing sophorolipid and magnolol, its preparation method and application. Background Technology

[0002] The skin's own secretions of oil and sweat, along with residue from daily sunscreens and makeup, can accumulate on the skin's surface if combined with dust and pollutants from the external environment without thorough cleansing. This can clog pores and increase the risk of skin allergies, especially for oily skin or those prone to acne, making them more susceptible to breakouts and pimples. Therefore, proper cleansing is essential. While common cleansing products are effective at removing makeup and dirt, their strong cleansing power can damage the skin barrier. Conversely, weaker cleansing products may fail to completely remove sebum, makeup, sunscreen, and environmental particles, leading to acne breakouts. Therefore, providing a cleansing product that combines cleansing and acne-fighting properties is a pressing issue that needs to be addressed. Summary of the Invention

[0003] In view of this, a composition containing sophorolipid and magnolol, its preparation method and application are proposed.

[0004] In a first aspect, the present invention provides a composition containing sophorolipid and magnolol, the composition comprising sophorolipid, magnolol and Cynanchum atratum extract in a mass ratio of 1:0.5-2.5:0.8-1.2.

[0005] Furthermore, the preparation method of the *Cynanchum paniculatum* extract is as follows: pulverize dried *Cynanchum paniculatum* to obtain *Cynanchum paniculatum* powder; mix the *Cynanchum paniculatum* powder with an extraction solvent, and sonicate to obtain an extract; centrifuge the extract, collect the supernatant, and dry it to obtain the *Cynanchum paniculatum* extract; The extraction solvent comprises betaine and lactic acid in a molar ratio of 1:1.3-1.8.

[0006] Furthermore, the water content of the extraction solvent is 20-30 wt%.

[0007] Furthermore, the ratio of the powder to the extraction solvent is 1g:10-20mL.

[0008] Furthermore, the ultrasonic treatment has a power of 200-300W, a temperature of 50-60℃, and a time of 15-25min.

[0009] Furthermore, the composition comprises sophorolipid, magnolol, and cynanchum atratum extract in a mass ratio of 1:2:1.

[0010] In a second aspect, the present invention provides a method for preparing the composition described in the first aspect, the method comprising the following steps: mixing the raw materials evenly to obtain the composition.

[0011] Thirdly, the present invention provides the use of the composition described in the first aspect in the preparation of cleaning products.

[0012] Furthermore, the composition is used in the cleaning product at an amount of 0.1-10 wt%.

[0013] Furthermore, the cleaning product is any one of makeup remover oil, makeup remover balm, facial cleanser, makeup remover water, shampoo, shower gel, soap, and hand sanitizer.

[0014] In this invention: Sophorolipids: Sophorolipids are microbial secondary metabolites produced by the fermentation process of Candida albicans, derived from honey, using sugar and vegetable oil as carbon sources. They have good surface activity and can effectively cleanse the skin and hair while reducing skin irritation.

[0015] Magnolia alcohol: Magnolia alcohol is a polyphenol extracted from Magnolia officinalis bark, which has certain whitening, antioxidant, anti-inflammatory and soothing effects.

[0016] Baiwei extract: Baiwei contains a large amount of saponins and polyphenolic compounds, which have certain anti-inflammatory, whitening, repairing and soothing effects.

[0017] Compared with the prior art, the present invention has the following beneficial effects: (1) The present invention combines sophorolipid, magnolol and white peony extract into a composition. The composition has good antibacterial, moisturizing and anti-inflammatory effects. Adding the composition to cleaning products can improve the antibacterial, anti-inflammatory, moisturizing and repairing effects of the cleaning products. (2) In the preparation method of Baiwei extract, the present invention uses betaine as hydrogen bond acceptor and lactic acid as hydrogen bond donor to prepare extraction solvent. By controlling the water content in the extraction solvent and the extraction process parameters, the plant cell wall of Baiwei is fully destroyed, so that the effective components can be better dissolved. The obtained Baiwei extract has a synergistic effect with sophorolipid and magnolol, thereby improving the antibacterial, anti-inflammatory, moisturizing and repairing effects of the composition. Attached Figure Description

[0018] Figure 1 The figures show a comparison of the biofilm removal effects of different compositions in Experiment 1 on Propionibacterium acnes. In the figures, A, F, and G represent the biofilm removal effects of comparative compositions 1-6 on Propionibacterium acnes, respectively, and G represents the biofilm removal effect of composition 3 on Propionibacterium acnes. Figure 2 This is a comparison chart showing the improvement in skin redness when the cleansing oil prepared in Example 1 was used in Experiment 5. Detailed Implementation

[0019] To make the objectives, technical solutions, and effects of this invention clearer and more explicit, the invention is further described in detail below. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

[0020] Unless otherwise specified, the experimental methods used in the examples are conventional methods; the materials and reagents used are commercially available unless otherwise specified.

[0021] Sophorolipids were purchased from Bolton (Shanghai) Biotechnology Co., Ltd. Magnoliaol was purchased from Hunan Heguang Biotechnology Co., Ltd.

[0022] Composition 1 The composition comprises sophorolipid, magnolol, and cynanchum atratum extract in a mass ratio of 1:0.5:0.8; The preparation method of the Baiwei extract includes the following steps: (1) Pulverize the dried white peony root and pass it through a 100-mesh sieve to obtain white peony root powder; (2) Mix the powder of Baiwei and the extraction solvent at a ratio of 1g:10mL and sonicate for 15min at a power of 200W and a temperature of 60℃ to obtain the extract; (3) After centrifuging the extract at 10,000 rpm / min for 3 min, collect the supernatant, dry it, and obtain the Baiwei extract; The extraction solvent comprises betaine and lactic acid in a molar ratio of 1:1.3 and has a water content of 20 wt%.

[0023] Composition 2 The composition comprises sophorolipid, magnolol, and cynanchum atratum extract in a mass ratio of 1:2.5:1.2; The preparation method of the Baiwei extract includes the following steps: (1) Pulverize the dried white peony root and pass it through a 100-mesh sieve to obtain white peony root powder; (2) Mix the powder of Baiwei and the extraction solvent at a ratio of 1g:20mL and sonicate for 25min at a power of 300W and a temperature of 50℃ to obtain the extract; (3) After centrifuging the extract at 10,000 rpm / min for 3 min, collect the supernatant, dry it, and obtain the Baiwei extract; The extraction solvent comprises betaine and lactic acid in a molar ratio of 1:1.8 and has a water content of 30 wt%.

[0024] Composition 3 The composition comprises sophorolipid, magnolol, and extract of Cynanchum paniculatum in a mass ratio of 1:2:1; The preparation method of the Baiwei extract includes the following steps: (1) Pulverize the dried white peony root and pass it through a 100-mesh sieve to obtain white peony root powder; (2) Mix the powder of Baiwei and the extraction solvent at a ratio of 1g:15mL and sonicate for 20min at a power of 250W and a temperature of 55℃ to obtain the extract; (3) After centrifuging the extract at 10,000 rpm / min for 3 min, collect the supernatant, dry it, and obtain the Baiwei extract; The extraction solvent comprises betaine and lactic acid in a molar ratio of 1:1.5 and has a water content of 25 wt%.

[0025] Comparative composition 1 Compared with composition 3, comparative composition 1 lacks sophorolipids, and the missing amount is made up by magnolol and cynanchum extract in a mass ratio of 2:1.

[0026] Comparative composition 2 Compared with composition 3, comparative composition 2 lacks Baiwei extract, and the missing amount is made up by sophorolipid and magnolol in a mass ratio of 1:2.

[0027] Comparative composition 3 Compared with composition 3, comparative composition 3 lacks magnolol, and the missing amount is made up by sophorolipid and cynanchum extract in a mass ratio of 1:1.

[0028] Comparative composition 4 Compared with composition 3, comparative composition 4 includes sophorolipid, magnolol and cynanchum atratum extract in a mass ratio of 1:3:1.

[0029] Comparative composition 5 Compared with composition 3, the only difference of comparative composition 5 is the extraction solvent used in the preparation method of Baiwei extract. The extraction solvent includes choline chloride and lactic acid in a molar ratio of 1:1.5 and has a water content of 25wt%. The remaining steps are the same as the preparation method of Baiwei extract of composition 3.

[0030] Comparative composition 6 Compared with composition 3, the only difference of comparative composition 6 is the extraction solvent used in the preparation method of the white peony extract. The extraction solvent includes betaine and lactic acid in a molar ratio of 1.5:1 and has a water content of 25wt%. The remaining steps are the same as the preparation method of the white peony extract of composition 3.

[0031] Experimental Example 1 Compositions 1-3 and comparative compositions 1-6 were prepared into test sample solutions with a concentration of 0.01 g / mL; the preparation method of the test sample solutions is as follows: The preparation methods of the test sample solutions of compositions 1-3 and comparative compositions 4-6 are as follows: first, dissolve magnolol using Tween-85, then add cynanchum atratum extract and sophorolipid, and finally add deionized water to dilute the composition to the final concentration; The preparation method of the test sample solution of Comparative Composition 1 is as follows: first, dissolve magnolol using Tween-85, then add Baiwei extract, and finally add deionized water to dilute the composition to the final concentration; The preparation method of the test sample solution of Comparative Composition 2 is as follows: first, dissolve magnolol using Tween-85, then add sophorolipid, and finally add deionized water to dilute the composition to the final concentration; The preparation method of the test sample solution of comparative composition 3 is as follows: dilute the extract of Cynanchum paniculatum and sophorolipid to the final concentration using deionized water; The sample solution to be tested was subjected to an antibacterial test, and the test bacteria were Staphylococcus aureus and Escherichia coli.

[0032] Test steps: (1) Preparation of antibacterial tablets: Take sterile filter paper, add 20 μL of the sample solution to be tested to each filter paper, place the filter paper flat in a clean sterile petri dish, and dry it in an oven at 37℃ for later use; (2) Inoculation of test bacteria: Use a sterile cotton swab to collect a concentration of 5×10⁻⁶ bacteria. 5 CFU / mL ~5×10 6 The test bacterial suspension of CFU / mL was spread on the surface of a nutrient agar plate. After each spread, the plate was rotated 60° and spread evenly 3 times. Finally, a cotton swab was used to spread around the edge of the plate. The plate was left to air dry for 5 minutes at room temperature.

[0033] (3) Placement of antibacterial tablets: Use sterile forceps to place the antibacterial tablets on the surface of the agar plate, with 4 tablets placed on each plate. The center of each antibacterial tablet should be at least 25 mm apart, and the distance from the periphery of the plate should be at least 15 mm. After placement, use sterile forceps to firmly attach the tablets to the surface of the agar plate. Cover the agar plate and incubate at 37℃ for 16-18 hours.

[0034] (4) Measurement of inhibition diameter: Select a uniform and completely sterile inhibition ring, measure the diameter of the inhibition ring with vernier calipers and record it. The diameter measurement of the inhibition ring is based on the outer edge of the inhibition ring. The test is repeated 3 times and the average value is calculated as the test result. The test results are shown in Table 1.

[0035] Table 1. Results of antibacterial tests against Staphylococcus aureus and Escherichia coli. Group Diameter of the inhibition zone of Staphylococcus aureus (mm) Diameter of the inhibition zone of Escherichia coli (mm) Composition 1 25.1 19.3 Composition 2 25.7 18.9 Composition 3 26.5 19.4 Comparative composition 1 17.5 10.9 Comparative composition 2 13.7 13.5 Comparative composition 3 16.2 11.7 Comparative composition 4 19.4 14.1 Comparative composition 5 23.6 16.2 Comparative composition 6 21.7 17.8 The experimental results show that compositions 1-3 of the present invention have good inhibitory effects on both Staphylococcus aureus and Escherichia coli.

[0036] By comparing the antibacterial test data of composition 3 and comparative compositions 1-3, it can be seen that the sophorolipid, magnolol and cynanchum extract used in this invention have a synergistic effect and have a good inhibitory effect on Staphylococcus aureus and Escherichia coli.

[0037] By comparing the antibacterial test data of composition 3 and composition 4, it can be seen that the combination of sophorolipid, magnolol and cynanchum extract in the present invention at a specific mass ratio can further enhance the inhibitory effect of the composition on Staphylococcus aureus and Escherichia coli.

[0038] By comparing the antibacterial test data of composition 3 and comparative compositions 5-6, it can be seen that the preparation method of the extract of *Cynanchum paniculatum* of the present invention can effectively extract the effective components of *Cynanchum paniculatum* that inhibit the growth of *Staphylococcus aureus* and *Escherichia coli*, thereby improving the antibacterial effect of the composition.

[0039] Experiment Example 2: Removal of Propionibacterium acnes biofilm (1) Biofilm preparation: Propionibacterium acnes ATCC 6919 was cultured to about OD600=1, diluted 1:50, transferred to 24-well plates, 2 mL per well, and cultured for 5 days.

[0040] (2) Washing the test sample with aqueous solution: Prepare the test sample solution with a concentration of 1 ppm by combining composition 3 and comparative compositions 1-6; the preparation method of the test sample solution is the same as that in Experimental Example 1. The pure water group serves as a control. The supernatant of the cultured strain is aspirated and 100 μL of the above test sample solution or pure water is added. Each group is divided into 4 replicates. The mixture is shaken on a shaker at 37°C and 200 rpm for 20 min. The test sample solution or pure water is then aspirated and washed once with pure water.

[0041] (3) Crystal violet staining: Add one drop of ammonium oxalate crystal violet working solution to the above well plate, add 500uL of water, stain for 2min, aspirate the staining solution, and wash with pure water 2-3 times.

[0042] (4) Take photos to record the biofilm residue. See Figure 1 In the figure, AF represents the effect of comparative compositions 1-6 on the removal of Propionibacterium acnes biofilm, and G represents the effect of composition 3 on the removal of Propionibacterium acnes biofilm.

[0043] Compared with composition 3, comparative compositions 1-3 lacked one of sophorolipids, magnolol, and Cynanchum atratum extract, respectively, resulting in a decreased clearance effect of the compositions on Propionibacterium acnes biofilm. The results indicate that sophorolipids, magnolol, and Cynanchum atratum extract used in this invention have a synergistic effect on the clearance of Propionibacterium acnes biofilm; the simultaneous use of all three significantly improves the clearance effect of the compositions on Propionibacterium acnes biofilm.

[0044] Compared to composition 3, the adjusted ingredient ratios in composition 4 resulted in a decreased removal effect of the composition on Propionibacterium acnes biofilm. The results indicate that the specific mass ratios of the ingredients used in this invention can further enhance the removal effect of the composition on Propionibacterium acnes biofilm.

[0045] Compared with composition 3, the adjustment of the preparation method of Baiwei extract in comparative compositions 5-6 resulted in a decrease in the scavenging effect of the compositions on Propionibacterium acnes biofilm. The results indicate that selecting the preparation method of Baiwei extract in this invention allows the obtained Baiwei extract to contain more effective components for scavenging Propionibacterium acnes biofilm, thereby improving the scavenging effect of the compositions on Propionibacterium acnes biofilm.

[0046] Application examples Composition 3 and comparative compositions 1-6 were added as active ingredients to the cleansing oil, respectively, and are referred to as Application Example 1 and Comparative Application Examples 1-6. The cleansing oil comprises the following ingredients by weight percentage: 30% ethylhexyl stearate, 15% cetyl ethylhexanoate, 25% C13-16 isoparaffin, 5% polysorbate-85, 10% sorbitol polyether-30 tetraoleate, 1% active ingredient and the balance deionized water; The preparation steps of the cleansing oil containing composition 3 and comparative compositions 4-6 are as follows: S1: According to the preset ratio, ethylhexyl stearate, cetyl ethylhexanoate, and C13-16 isoparaffin are mixed, heated to 80°C and stirred evenly to obtain phase A; S2: Sorbitol polyether-30 tetraoleate, polysorbate-85 and magnolol are added to phase A, stirred and mixed evenly, and then cooled to 50°C to obtain phase B; S3: Add sophorolipids, white peony extract, and deionized water to phase B, stir and mix evenly; cool and discharge.

[0047] The preparation steps of the cleansing oil containing comparative composition 1 are as follows: S1: According to the preset ratio, ethylhexyl stearate, cetyl ethylhexanoate, and C13-16 isoparaffin are mixed, heated to 80°C and stirred evenly to obtain phase A; S2: Sorbitol polyether-30 tetraoleate, polysorbate-85 and magnolol are added to phase A, stirred and mixed evenly, and then cooled to 50°C to obtain phase B; S3: Add the extract of Cynanchum paniculatum and deionized water to phase B, stir and mix evenly; cool down and discharge.

[0048] The preparation steps of the cleansing oil containing comparative composition 2 are as follows: S1: According to the preset ratio, ethylhexyl stearate, cetyl ethylhexanoate, and C13-16 isoparaffin are mixed, heated to 80°C and stirred evenly to obtain phase A; S2: Sorbitol polyether-30 tetraoleate, polysorbate-85 and magnolol are added to phase A, stirred and mixed evenly, and then cooled to 50°C to obtain phase B; S3: Add sophorolipid and deionized water to phase B, stir and mix evenly; cool and discharge.

[0049] The preparation steps of the cleansing oil containing comparative composition 3 are as follows: S1: According to the preset ratio, ethylhexyl stearate, cetyl ethylhexanoate, and C13-16 isoparaffin are mixed, heated to 80°C and stirred evenly to obtain phase A; S2: Sorbitol polyether-30 tetraoleate and polysorbate-85 are added to phase A, stirred and mixed evenly, and then cooled to 50°C to obtain phase B; S3: Add sophorolipids, white peony extract, and deionized water to phase B, stir and mix evenly; cool and discharge.

[0050] Experimental Example 3 Moisturizing effect test Thirty-five female volunteers aged 22-35 were randomly selected and divided into seven groups of five. They washed their faces with water and patted them dry with tissues. The skin moisture content before makeup application was measured. After the test, foundation, eyebrow pencil, eyeliner, and lipstick were applied to the face. Makeup was removed using the cleansing oils from Application Example 1 and Comparative Examples 1-6, respectively. A sufficient amount of cleansing oil was applied to the face and massaged in circular motions for 15 seconds, emulsified with water for 30 seconds, and then rinsed off with water. The face was then patted dry with tissues. The skin moisture content 5 minutes after cleansing was measured using a Croneometer CM825, and the rate of change in moisture was calculated. The results are shown in Table 2.

[0051] Moisture change rate = (facial skin moisture content 5 minutes after cleansing - facial skin moisture content before makeup application) / facial skin moisture content before makeup application × 100%.

[0052] Table 2. Results of Moisturizing Effect Test Group Moisture change rate (%) Application Example 1 2.49 Comparative Application Example 1 1.23 Comparative Application Example 2 1.16 Comparative Application Example 3 0.94 Comparative Application Example 4 1.76 Comparative Application Example 5 2.01 Comparative Application Example 6 2.12 The higher the value of the moisture change rate in Table 2, the better the moisturizing effect of the composition.

[0053] Experimental results show that adding the composition of this invention to cleansing oil can quickly remove facial makeup. Furthermore, within 5 minutes after makeup removal, skin moisture levels increase compared to before makeup removal. This indicates that using the composition of this invention for cleansing not only provides good cleansing effects but also replenishes the skin's moisture, effectively preventing moisture evaporation from the skin surface and relieving dryness and tightness after makeup removal, thus improving the gentleness of the cleansing oil.

[0054] Compared to Application Example 1, Comparative Application Examples 1-4 adjusted the raw material composition of the composition, resulting in a decrease in the moisturizing effect of the cleansing oil. This may be because sophorolipid, magnolol and peony extract have a synergistic effect, and adding them to the cleansing products can play a moisturizing and repairing role.

[0055] Compared with Application Example 1, the preparation method of Baiwei extract in Comparative Application Examples 5-6 was adjusted, which resulted in a decrease in the moisturizing effect of the cleansing oil. This may be because the preparation method affects the composition and content of active ingredients in Baiwei extract, thereby affecting the moisturizing and repairing effects of the cleansing oil.

[0056] Test Example 4 Ten female volunteers and five male volunteers were selected for the patch experiment.

[0057] The cleansing oil prepared using Example 1 was subjected to a human skin occlusion test in accordance with the 2015 edition of the "Cosmetic Safety Technical Specifications".

[0058] Procedure: Select a qualified patch tester and apply the test substance to the normal skin on the flexor side of the subject's forearm using a closed patch test method. The amount used is about 0.02g to 0.03g. Remove the test substance after 24 hours and observe the skin reaction at 30min, 24h and 48h after removal.

[0059] Table 3. Criteria for judging the degree of reaction at the test site: Phenomenon determination Rating levels No skin reaction - 0 The skin has light red spots and no maceration. ± 1 The skin presents with erythema, infiltration, and papules. + 2 The skin presents with erythema, edema, papules, and small blisters. ++ 3 Large blisters appeared on the skin, which was covered with erythema and edema. +++ 4 Table 4. Results of patch test

[0060] The test results show that the composition prepared by the present invention has no obvious irritation or sensitization to the human body, that is, it has good safety performance.

[0061] Experimental Example 5 By recording and detecting skin changes in volunteers after 28 consecutive days of use of the cleansing oil prepared in Example 1, the soothing and repairing effects of the composition and cleansing oil of this invention were investigated. The specific test method is as follows: Volunteers used the cleansing oil from Example 1 once daily, 5g each time, for 28 consecutive days. The cheeks of the volunteers were selected as the test sites, and VISIA tests were performed before use and on the 28th day of continuous use. The comparison of redness areas before and after 28 days of continuous use for one volunteer is shown below. Figure 2 As shown in the comparison images of volunteers' skin on days 0 and 28, it can be seen that adding the composition of this invention to the makeup remover oil and using it continuously for 28 days can effectively improve skin redness. This may be because sophorolipid, magnolol and peony extract have a synergistic effect, which achieves a soothing and repairing effect by inhibiting the expression and activation of TRPV1 receptors in keratinocytes and nerve cells.

[0062] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A composition containing sophorolipid and magnolol, characterized in that, The composition comprises sophorolipid, magnolol, and cynanchum atratum extract in a mass ratio of 1:0.5-2.5:0.8-1.

2.

2. The composition containing sophorolipid and magnolol as described in claim 1, characterized in that, The preparation method of the Baiwei extract is as follows: pulverize dried Baiwei to obtain Baiwei powder; mix Baiwei powder and extraction solvent, and sonicate to obtain extract; centrifuge the extract, collect the supernatant, and dry to obtain the Baiwei extract; The extraction solvent comprises betaine and lactic acid in a molar ratio of 1:1.3-1.

8.

3. The composition containing sophorolipid and magnolol as described in claim 2, characterized in that, The water content of the extraction solvent is 20-30 wt%.

4. The composition containing sophorolipid and magnolol as described in claim 2, characterized in that, The ratio of the powder to the extraction solvent is 1g:10-20mL.

5. The composition containing sophorolipid and magnolol as described in claim 2, characterized in that, The ultrasonic treatment has a power of 200-300W, a temperature of 50-60℃, and a time of 15-25min.

6. The composition containing sophorolipid and magnolol as described in claim 1, characterized in that, The composition comprises sophorolipid, magnolol, and cynanchum atratum extract in a mass ratio of 1:2:

1.

7. The method for preparing the composition containing sophorolipid and magnolol as described in any one of claims 1-6, characterized in that, The preparation method includes the following steps: mixing the raw materials evenly to obtain the composition.

8. The use of the composition containing sophorolipid and magnolol as described in any one of claims 1-6 in the preparation of cleaning products.

9. The application as described in claim 8, characterized in that, The composition containing sophorolipid and magnolol is used in cleaning products at an amount of 0.1-10 wt%.

10. The application as described in claim 8, characterized in that, The cleaning product is any one of the following: makeup remover oil, makeup remover balm, facial cleanser, makeup remover water, shampoo, shower gel, soap, and hand sanitizer.

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