Stem cell culture solution constant-temperature oscillation shaking table device

Abstract

The invention relates to the technical field of cell culture, and discloses a stem cell culture solution constant-temperature oscillation shaking table device which comprises a constant-temperature box, a controller is arranged on one side of the constant-temperature box, a fixing module is installed in the constant-temperature box, and the constant-temperature box is provided with an oscillation assembly, a fixing assembly and a turbulent flow assembly; according to the invention, the oscillation assembly is arranged, a first driving motor is used as a driving part, and when a driving gear rotates clockwise, a second driven gear is engaged with a one-way ratchet wheel compound gear, so that a fixing seat drives a stem cell culture solution on a fixing module to swing circularly in a one-way manner; the second driven gear is meshed with the first driven gear, the first driven gear can rotate, meanwhile, the third driven gear is driven to rotate, the connecting rod is driven to move, under the matching action of the one-way ratchet wheel compound gear, the rocker arm stops and rotates once, different oscillation modes are generated, and the oscillation effect on the stem cell culture solution is improved.

Description

Technical Field

[0001] This invention relates to the field of cell culture technology, specifically to a constant temperature shaking incubator for stem cell culture medium. Background Technology

[0002] Stem cell culture is a crucial step in biological and medical research, providing scientists with a unique platform to delve into the mysteries of life and the pathogenesis of diseases. Through stem cell culture, researchers can simulate the processes of cell growth and differentiation in vitro, which is significant for understanding basic cell functions, disease development, and potential treatments. However, existing stem cell culture shakers present the following problems in use:

[0003] During the shaking process of cell culture medium, the shaking is achieved by a rocker arm driving a fixed stem cell culture medium container at the top to perform a single cycle of shaking. Therefore, this single shaking mode often results in poor shaking effect when shaking stem cell culture medium, failing to fully meet the diverse needs of stem cell culture. Furthermore, most current stem cell culture medium shakers are designed with only a single-layer structure, which limits the number of cells that can be shaken at one time, further restricting experimental efficiency and scale. Summary of the Invention

[0004] The purpose of this invention is to provide a constant temperature shaking incubator for stem cell culture medium to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution:

[0006] A constant-temperature shaking incubator for stem cell culture includes a constant-temperature chamber with a glass door at the front and a controller on one side. A fixing module is installed inside the chamber, and the chamber is equipped with a shaking component, a fixing component, and a turbulence-inducing component.

[0007] The oscillation assembly is located inside the constant temperature chamber. The oscillation assembly includes a base, several rocker arms, a fixed base, and a first drive motor. The first drive motor is used as the driving component, and gear transmission is used to drive the rocker arms to drive the fixed base to vibrate, thereby oscillating the culture medium installed on the fixed module on the fixed base.

[0008] The fixing component is disposed between the fixing module and the oscillation component. The fixing component includes a slot, a first fixing ear, a first insertion hole, a second fixing ear, a second insertion hole, and a fixing pin. The fixing module is installed by the cooperation of the slot, the first fixing ear, the first insertion hole, the second fixing ear, the second insertion hole, and the fixing pin, so as to oscillate the culture medium and remove it after oscillation.

[0009] The airflow disturbance component is located on the top of the constant temperature chamber. The airflow disturbance component includes a fixed disk and several airflow disturbance blades. The fixed disk rotates, which drives the multiple airflow disturbance blades to rotate, thereby disturbing the air inside the constant temperature chamber and improving the uniformity of air distribution inside the constant temperature chamber.

[0010] Optionally, the oscillation assembly further includes a driving gear, a one-way ratchet compound gear, a first driven gear, an arc-shaped slide block, a slider, a second driven gear, a third driven gear, and a connecting rod. The driving gear is fixedly installed at one end of the output shaft of the first drive motor, the one-way ratchet compound gear is fixedly installed at one end of the shaft connecting the rocker arm and the base, the first driven gear and the third driven gear are rotatably connected to one side of the base, the arc-shaped slide block is fixedly installed on one side of the base, the slider is slidably connected to the inner side of the arc-shaped slide block, the second driven gear is rotatably connected to one side of the slider, and the connecting rod is movably installed between the second driven gear and the one-way ratchet compound gear.

[0011] Optionally, the base is fixedly installed on the bottom of the inner side of the constant temperature chamber, and one end of the bottom of several rocker arms is rotatably connected to the top of the base, and one end of the top of several rocker arms is rotatably connected to the bottom of the fixed base, and the first drive motor is fixedly installed on the top of the base.

[0012] Optionally, the second driven gear meshes with the driving gear. When the driving gear rotates clockwise, the second driven gear meshes with the one-way ratchet compound gear. When the driving gear rotates counterclockwise, the second driven gear meshes with the first driven gear. The first driven gear meshes with the third driven gear. One end of the connecting rod is rotatably connected to one edge of the third driven gear, and the other end of the connecting rod is rotatably connected to one edge of the one-way ratchet compound gear.

[0013] Optionally, the fixing component further includes a U-shaped frame, a limiting rod, and a limiting groove. One end of the U-shaped frame is rotatably connected to one end of the fixing pin, the limiting rod is fixedly connected to one end of the U-shaped frame, the limiting groove is formed at one end of the fixing pin, and one end of the limiting rod matches the one end of the limiting groove.

[0014] Optionally, the slot is formed on the top of the fixing base, the first fixing ear is fixedly installed on one side of the bottom of the fixing module, the first insertion hole is formed at one end of the first fixing ear, the second fixing ear is fixedly connected to one side of the fixing base, the second insertion hole is formed at one end of the second fixing ear, the diameter of the fixing pin is matched with the inner diameter of the first insertion hole and the second insertion hole respectively, and one end of the fixing pin is respectively sleeved on the inner side of the first insertion hole and the second insertion hole.

[0015] Optionally, the turbulence-disrupting component further includes a second drive motor, which is fixedly mounted on the top of the constant temperature chamber.

[0016] Optionally, the fixed disk is fixedly installed at one end of the output shaft of the second drive motor. The fixed disk is located inside the constant temperature chamber, and several of the baffles are arranged in a ring array at the bottom of the fixed disk.

[0017] Optionally, the fixing module includes a lower support plate, a support column, an upper support plate, and several fixing clips. The lower support plate is located on the top of the fixing base, the support column is fixedly connected to the top of the lower support plate, the upper support plate is fixedly installed on the top of the support column, and several fixing clips are fixedly installed on the top of the lower support plate and the upper support plate.

[0018] Optionally, the edge shape of the lower support plate matches the shape of the slot, and several of the fixing clips are arranged in a circular array on the top of the lower support plate and the upper support plate.

[0019] The present invention has at least the following beneficial effects:

[0020] (1) This solution sets up an oscillation component. Specifically, the first drive motor is used as the drive component. When the active gear rotates clockwise, the second driven gear meshes with the one-way ratchet compound gear, causing the fixed seat to drive the stem cell culture medium on the fixed module to oscillate in one direction. At the same time, when the active gear rotates counterclockwise, the second driven gear meshes with the first driven gear. At this time, the first driven gear will rotate, and at the same time drive the third driven gear to rotate, driving the connecting rod to move. Under the cooperation of the one-way ratchet compound gear, the rocker arm can stop once and rotate once, producing different oscillation modes and improving the oscillation effect on the stem cell culture medium.

[0021] (2) This scheme sets up a fixing module. Specifically, the fixing module is set up with a lower support plate and an upper support plate, and multiple fixing clips are installed on the lower support plate and the upper support plate to fix the culture bottles of stem cell culture medium. The double-layer design allows more stem cell culture medium to be shaken at one time, which improves experimental efficiency.

[0022] (3) This solution uses a fixing component to fix the lower support plate edge of the fixing module by using a slot to hold it in place. At the same time, the fixing pin cooperates with the first and second sockets to connect the first fixing ear and the second fixing ear, thus fixing the fixing module. When removing it, the fixing pin can be pulled out to remove the fixing module, so that the fixing module can be easily installed and removed, avoiding obstruction for storing and retrieving stem cell culture medium.

[0023] (4) This solution sets up a turbulence component. Specifically, the second drive motor drives the fixed disk to rotate. The rotating fixed disk and turbulence blades effectively promote the flow and uniformity of air distribution inside the constant temperature chamber, reduce the temperature gradient, and ensure that the entire constant temperature chamber maintains a stable temperature environment, thereby further improving the consistency and reliability of stem cell culture. Attached Figure Description

[0024] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0025] Figure 1 This is a schematic diagram of the top side structure of the present invention;

[0026] Figure 2 This is a schematic diagram of the bottom side structure of the present invention;

[0027] Figure 3 This is a schematic diagram of a partial internal structure of the present invention;

[0028] Figure 4 This is a schematic diagram of the bottom structure of the oscillation component of the present invention;

[0029] Figure 5 This is a schematic diagram of one side of the oscillation component of the present invention;

[0030] Figure 6 This is a schematic diagram of the fixed module structure of the present invention;

[0031] Figure 7 For the present invention Figure 3 A magnified view of a portion of point A in the middle.

[0032] The attached diagram lists the components represented by each number as follows:

[0033] 1. Constant temperature chamber; 2. Glass door; 3. Controller; 401. Base; 402. Rocker arm; 403. Fixed seat; 404. First drive motor; 405. Drive gear; 406. One-way ratchet compound gear; 407. First driven gear; 408. Arc-shaped slide; 409. Slider; 410. Second driven gear; 411. Third driven gear; 412. Connecting rod; 5. Fixed module; 501. Lower support plate; 502. Support column; 503. Upper support plate; 504. Fixed clamp; 601. Slot; 602. First fixed ear; 603. First insertion hole; 604. Second fixed ear; 605. Second insertion hole; 606. Fixed pin; 607. U-shaped frame; 608. Limiting rod; 609. Limiting groove; 701. Second drive motor; 702. Fixed plate; 703. Deflector blade. Detailed Implementation

[0034] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0035] Please see Figures 1-7 The present invention provides a constant temperature shaking incubator device for stem cell culture medium, including a constant temperature chamber 1, a glass door 2 on the front side of the constant temperature chamber 1, a controller 3 on one side of the constant temperature chamber 1, a fixing module 5 installed inside the constant temperature chamber 1, and the constant temperature chamber 1 is respectively provided with a shaking component, a fixing component and a turbulence component.

[0036] It should be noted that controller 3, as the core control component of the entire stem cell culture medium constant-temperature shaking incubator, is closely connected to the constant-temperature chamber 1. Controller 3 has a built-in microprocessor or other intelligent control unit responsible for receiving user-set parameters such as temperature and oscillation frequency, and monitoring the actual state inside the constant-temperature chamber 1 in real time through internal circuits and sensors. Based on the difference between the preset conditions and the actual state, controller 3 precisely regulates the operation of the heating / cooling system and the oscillation components inside the constant-temperature chamber 1 to ensure that the stem cell culture medium is cultured in the optimal environment. The glass door 2 is made of transparent glass, allowing researchers to directly observe the state and oscillation of the internal stem cell culture medium without opening the constant-temperature chamber 1. This helps researchers monitor the experimental progress and cell growth status at any time, avoiding frequent opening of the chamber and interference with the constant-temperature environment. The glass door 2 uses double-layered laminated glass, which has excellent thermal insulation performance, effectively reducing heat exchange between the inside of the constant-temperature chamber 1 and the external environment. This helps maintain the stability of the internal temperature of the constant-temperature chamber 1, ensuring that stem cells are cultured under suitable temperature conditions.

[0037] The oscillation assembly is located inside the constant temperature chamber 1. The oscillation assembly includes a base 401, several rocker arms 402, a fixed base 403 and a first drive motor 404. The first drive motor 404 is used as the driving component, and the rocker arms 402 are driven by gear transmission to drive the fixed base 403 to vibrate, thereby oscillating the culture medium installed on the fixed module 5 on the fixed base 403.

[0038] In some embodiments, see Figure 4 , Figure 5The oscillation assembly also includes a drive gear 405, a one-way ratchet compound gear 406, a first driven gear 407, an arc-shaped slide 408, a slider 409, a second driven gear 410, a third driven gear 411, and a connecting rod 412. The drive gear 405 is fixedly mounted on one end of the output shaft of the first drive motor 404. The one-way ratchet compound gear 406 is fixedly mounted on one end of the shaft connecting the rocker arm 402 and the base 401. The first driven gear 407 and the third driven gear 411 are rotatably connected to one side of the base 401. The arc-shaped slide 408 is fixedly mounted on one side of the base 401. The slider 409 is slidably connected to the inner side of the arc-shaped slide 408. The second driven gear 410 is rotatably connected to one side of the slider 409. The connecting rod 412 is movably mounted between the second driven gear 410 and the one-way ratchet compound gear 406. The base 401 is fixedly installed on the bottom of the inner side of the constant temperature chamber 1. One end of several rocker arms 402 is rotatably connected to the top of the base 401, and the top of several rocker arms 402 is rotatably connected to the bottom of the fixed seat 403. The first drive motor 404 is fixedly installed on the top of the base 401. The second driven gear 410 meshes with the drive gear 405. When the drive gear 405 rotates clockwise, the second driven gear 410 meshes with the one-way ratchet compound gear 406. When the drive gear 405 rotates counterclockwise, the second driven gear 410 meshes with the first driven gear 407. The first driven gear 407 meshes with the third driven gear 411. One end of the connecting rod 412 is rotatably connected to one side edge of the third driven gear 411, and the other end of the connecting rod 412 is rotatably connected to one side edge of the one-way ratchet compound gear 406.

[0039] Among them, the one-way ratchet compound gear 406 is a special gear structure that combines the functions of a ratchet and a gear. It mainly consists of a ratchet part and a spur gear part, which are tightly joined together by bolts. The ratchet part has a series of ratchet teeth, which can only mesh with the corresponding pawl in one direction. The gear part has a standard gear tooth profile and is used to interact with other gears.

[0040] It should be noted that, using the first drive motor 404 as the driving component, when the driving gear 405 rotates clockwise, it meshes with the second driven gear 410, forcing the slider 409 to move towards the one-way ratchet compound gear 406. This forces the second driven gear 410 to mesh with the gear portion of the one-way ratchet compound gear 406, causing the fixed base 403 to drive the stem cell culture medium on the fixed module 5 to circulate and shake in one direction. Simultaneously, when the driving gear 405 rotates counterclockwise, it forces the slider 409 to move towards the first driven gear 404. The movement of the driven gear 407 forces the second driven gear 410 to mesh with the first driven gear 407. At this time, the first driven gear 407 will rotate, which in turn drives the third driven gear 411 to rotate, causing the connecting rod 412 to move. Under the cooperation of the connecting rod 412 and the ratchet part of the one-way ratchet compound gear 406, the gear part of the one-way ratchet compound gear 406 is driven, so that the rocker arm 402 can stop and rotate once, producing different oscillation modes and improving the oscillation effect on the stem cell culture medium.

[0041] A fixing component is disposed between the fixing module 5 and the oscillation component. The fixing component includes a slot 601, a first fixing ear 602, a first insertion hole 603, a second fixing ear 604, a second insertion hole 605, and a fixing pin 606. The fixing module 5 is installed by the cooperation of the slot 601, the first fixing ear 602, the first insertion hole 603, the second fixing ear 604, the second insertion hole 605, and the fixing pin 606, so as to oscillate the culture medium and remove it after oscillation.

[0042] In some embodiments, see Figure 3 , Figure 7 The fixing assembly also includes a U-shaped frame 607, a limiting rod 608, and a limiting groove 609. One end of the U-shaped frame 607 is rotatably connected to one end of the fixing pin 606. The limiting rod 608 is fixedly connected to one end of the U-shaped frame 607. The limiting groove 609 is opened at one end of the fixing pin 606. One end of the limiting rod 608 matches one end of the limiting groove 609. The slot 601 is opened at the top of the fixing base 403. The first fixing ear 602 is fixedly installed on one side of the bottom of the fixing module 5. The first insertion hole 603 is opened at one end of the first fixing ear 602. The second fixing ear 604 is fixedly connected to one side of the fixing base 403. The second insertion hole 605 is opened at one end of the second fixing ear 604. The diameter of the fixing pin 606 matches the inner diameter of the first insertion hole 603 and the second insertion hole 605 respectively, and one end of the fixing pin 606 is sleeved inside the first insertion hole 603 and the second insertion hole 605 respectively.

[0043] The U-shaped bracket 607, the limiting rod 608, and the limiting groove 609 provided in the fixing pin 606 are used to prevent the fixing pin 606 from disengaging from the first insertion hole 603 and the second insertion hole 605. When fixing, the U-shaped bracket 607 is folded so that the limiting rod 608 is inserted into the limiting groove 609, forming a blockage at one end of the fixing pin 606 to prevent it from disengaging.

[0044] It should be noted that during installation, the slot 601 is used to hold the edge of the lower support plate 501 of the fixing module 5, and the fixing pin 606 cooperates with the first insertion hole 603 and the second insertion hole 605 to connect the first fixing ear 602 and the second fixing ear 604, thereby fixing the fixing module 5. When removing it, the fixing pin 606 can be pulled out to remove the fixing module 5, so that the fixing module 5 can be easily installed and removed, avoiding obstruction for storing and accessing stem cell culture medium.

[0045] The turbulence-dispersing component is located on the top of the constant temperature chamber 1. The turbulence-dispersing component includes a fixed disk 702 and several turbulence-dispersing blades 703. The fixed disk 702 rotates, which drives the multiple turbulence-dispersing blades 703 to rotate, thereby turbulenting the air inside the constant temperature chamber 1 and improving the uniformity of air distribution inside the constant temperature chamber 1.

[0046] In some embodiments, see Figure 1 , Figure 2 The turbulence assembly also includes a second drive motor 701, which is fixedly installed on the top of the constant temperature chamber 1. A fixed disk 702 is fixedly installed on one end of the output shaft of the second drive motor 701. The fixed disk 702 is located inside the constant temperature chamber 1, and several turbulence blades 703 are arranged in a ring array at the bottom of the fixed disk 702.

[0047] It should be noted that the fixed disk 702 is driven to rotate by the second drive motor 701. The rotating fixed disk 702 and the baffle blades 703 effectively promote the flow and uniform distribution of air inside the constant temperature chamber 1, reduce the temperature gradient, and ensure that the entire constant temperature chamber 1 maintains a stable temperature environment, thereby further improving the consistency and reliability of stem cell culture.

[0048] In some embodiments, see Figure 3 , Figure 6 The fixing module 5 includes a lower support plate 501, a support column 502, an upper support plate 503, and several fixing clips 504. The lower support plate 501 is located on the top of the fixing base 403. The support column 502 is fixedly connected to the top of the lower support plate 501. The upper support plate 503 is fixedly installed on the top of the support column 502. Several fixing clips 504 are fixedly installed on the top of the lower support plate 501 and the upper support plate 503. The edge shape of the lower support plate 501 matches the shape of the slot 601. Several fixing clips 504 are arranged in a ring array on the top of the lower support plate 501 and the upper support plate 503.

[0049] Depending on the internal height of the constant temperature chamber 1, it may or may not only be equipped with a lower support plate 501 and an upper support plate 503. In specific implementation, the fixing module 5 may be equipped with more support plates, preferably with a double layer.

[0050] It should be noted that the fixing module 5 is equipped with a lower support plate 501 and an upper support plate 503, and multiple fixing clips 504 are installed on the lower support plate 501 and the upper support plate 503 to fix the culture flasks of stem cell culture medium. The double-layer design allows for the shaking of more stem cell culture medium at one time, thus improving experimental efficiency.

[0051] The workflow and principle of this invention are as follows: First, the experimenter fixes the culture flask containing stem cell culture medium onto the fixing clamp 504 of the fixing module 5. Then, the slot 601 is used to hold the edge of the lower support plate 501 of the fixing module 5. At the same time, the fixing pin 606 is passed through the first insertion hole 603 of the first fixing ear 602 and the second insertion hole 605 of the second fixing ear 604 to achieve a stable connection between the fixing module 5 and the fixing base 403. To prevent the fixing pin 606 from falling off, it can be locked using the structure of the U-shaped frame 607, the limiting rod 608, and the limiting groove 609 to install the fixing module 5 on the fixing base 403 of the oscillation assembly. Then, the experimenter sets the required parameters such as temperature and oscillation frequency through the controller 3. The controller 3, as the core control component of the entire device, monitors the actual state inside the constant temperature chamber 1 in real time. Based on the difference between preset conditions and the actual state, it precisely controls the operation of the heating / cooling system and the oscillation component within the constant temperature chamber 1. During oscillation, the first drive motor 404 serves as the driving component. When the active gear 405 rotates clockwise, it meshes with the second driven gear 410, forcing the slider 409 to move towards the one-way ratchet compound gear 406. This forces the second driven gear 410 to mesh with the gear portion of the one-way ratchet compound gear 406, causing the fixed seat 403 to drive the stem cell culture medium on the fixed module 5 to oscillate in a one-way cycle. Simultaneously, when the active gear 405 rotates counterclockwise, it forces the slider 409 to move towards the first driven gear 407, forcing the second driven gear 410 to mesh with the first driven gear 407. At this time, the first driven gear 407 will rotate. The third driven gear 411 rotates, causing the connecting rod 412 to move. The connecting rod 412, in conjunction with the ratchet portion of the one-way ratchet compound gear 406, drives the gear portion of the one-way ratchet compound gear 406, allowing the rocker arm 402 to pause and rotate once, generating different oscillation patterns and improving the oscillation effect on the stem cell culture medium. During oscillation, the second drive motor 701 drives the fixed disk 702 to rotate, and the baffles 703 at the bottom of the fixed disk 702 rotate accordingly, turbulenting the air inside the incubator 1, reducing the temperature gradient, and ensuring a stable temperature environment inside the entire incubator 1. Throughout the oscillation culture process, the experimenter can directly observe the state of the stem cell culture medium and the oscillation through the glass door 2 on the front side of the incubator 1. After the oscillation culture is completed, the experimenter can pull out the fixing pin 606 and easily remove the fixing module 5 from the oscillation assembly. Then, the culture flask can be removed from the fixing module 5 for subsequent processing or analysis.

[0052] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0053] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A constant-temperature shaking incubator for stem cell culture medium, characterized in that, The system includes a constant temperature chamber (1), a glass door (2) on the front of the constant temperature chamber (1), a controller (3) on one side of the constant temperature chamber (1), a fixed module (5) installed inside the constant temperature chamber (1), and an oscillation component, a fixing component, and a turbulence component respectively provided in the constant temperature chamber (1). The oscillation assembly is located inside the constant temperature chamber (1). The oscillation assembly includes a base (401), several rocker arms (402), a fixed base (403), and a first drive motor (404). The first drive motor (404) is used as the driving component, and the rocker arms (402) are driven by gear transmission to drive the fixed base (403) to vibrate, thereby oscillating the culture medium installed on the fixed module (5) on the fixed base (403). The fixing component is disposed between the fixing module (5) and the oscillation component. The fixing component includes a slot (601), a first fixing ear (602), a first insertion hole (603), a second fixing ear (604), a second insertion hole (605), and a fixing pin (606). The fixing module (5) is installed by the cooperation of the slot (601), the first fixing ear (602), the first insertion hole (603), the second fixing ear (604), the second insertion hole (605), and the fixing pin (606) so as to oscillate the culture medium and remove it after oscillation. The turbulence-disrupting component is located on the top of the constant temperature chamber (1). The turbulence-disrupting component includes a fixed disk (702) and several turbulence-disrupting blades (703). The fixed disk (702) rotates, driving the multiple turbulence-disrupting blades (703) to rotate, thereby turbulenting the air inside the constant temperature chamber (1) and making the air inside the constant temperature chamber (1) flow, thus improving the uniformity of air distribution inside the constant temperature chamber (1).

2. The constant temperature shaking incubator device for stem cell culture medium according to claim 1, characterized in that: The oscillation assembly further includes a driving gear (405), a one-way ratchet compound gear (406), a first driven gear (407), an arc-shaped slide (408), a slider (409), a second driven gear (410), a third driven gear (411), and a connecting rod (412). The driving gear (405) is fixedly mounted on one end of the output shaft of the first drive motor (404), and the one-way ratchet compound gear (406) is fixedly mounted on the rocker arm (402) connected to the base (401). At one end of the shaft, the first driven gear (407) and the third driven gear (411) are rotatably connected to one side of the base (401), the arc-shaped slide (408) is fixedly installed on one side of the base (401), the slider (409) is slidably connected to the inner side of the arc-shaped slide (408), the second driven gear (410) is rotatably connected to one side of the slider (409), and the connecting rod (412) is movably installed between the second driven gear (410) and the one-way ratchet compound gear (406).

3. The constant temperature shaking incubator device for stem cell culture medium according to claim 1, characterized in that: The base (401) is fixedly installed on the bottom of the inner side of the constant temperature box (1). One end of several rocker arms (402) is rotatably connected to the top of the base (401), and one end of several rocker arms (402) is rotatably connected to the bottom of the fixed seat (403). The first drive motor (404) is fixedly installed on the top of the base (401).

4. The constant temperature shaking incubator device for stem cell culture medium according to claim 2, characterized in that: The second driven gear (410) meshes with the driving gear (405). When the driving gear (405) rotates clockwise, the second driven gear (410) meshes with the one-way ratchet compound gear (406). When the driving gear (405) rotates counterclockwise, the second driven gear (410) meshes with the first driven gear (407), and the first driven gear (407) meshes with the third driven gear (411). One end of the connecting rod (412) is rotatably connected to one side edge of the third driven gear (411), and the other end of the connecting rod (412) is rotatably connected to one side edge of the one-way ratchet compound gear (406).

5. The constant temperature shaking incubator device for stem cell culture medium according to claim 1, characterized in that: The fixing assembly also includes a U-shaped frame (607), a limiting rod (608), and a limiting groove (609). One end of the U-shaped frame (607) is rotatably connected to one end of the fixing pin (606), the limiting rod (608) is fixedly connected to one end of the U-shaped frame (607), and the limiting groove (609) is opened at one end of the fixing pin (606). The limiting rod (608) matches one end of the limiting groove (609).

6. The constant temperature shaking incubator device for stem cell culture medium according to claim 1, characterized in that: The slot (601) is opened on the top of the fixing base (403), the first fixing ear (602) is fixedly installed on one side of the bottom of the fixing module (5), the first insertion hole (603) is opened at one end of the first fixing ear (602), the second fixing ear (604) is fixedly connected to one side of the fixing base (403), the second insertion hole (605) is opened at one end of the second fixing ear (604), the diameter of the fixing pin (606) is matched with the inner diameter of the first insertion hole (603) and the second insertion hole (605) respectively, and one end of the fixing pin (606) is respectively sleeved on the inner side of the first insertion hole (603) and the second insertion hole (605).

7. The constant temperature shaking incubator device for stem cell culture medium according to claim 1, characterized in that: The turbulence assembly also includes a second drive motor (701), which is fixedly mounted on the top of the constant temperature chamber (1).

8. The constant temperature shaking incubator device for stem cell culture medium according to claim 7, characterized in that: The fixed disk (702) is fixedly installed at one end of the output shaft of the second drive motor (701). The fixed disk (702) is located inside the constant temperature chamber (1). Several of the baffle blades (703) are arranged in a ring array at the bottom of the fixed disk (702).

9. The constant temperature shaking incubator device for stem cell culture medium according to claim 1, characterized in that: The fixing module (5) includes a lower support plate (501), a support column (502), an upper support plate (503), and several fixing clips (504). The lower support plate (501) is located on the top of the fixing base (403). The support column (502) is fixedly connected to the top of the lower support plate (501). The upper support plate (503) is fixedly installed on the top of the support column (502). Several fixing clips (504) are fixedly installed on the top of the lower support plate (501) and the upper support plate (503).

10. The constant temperature shaking incubator device for stem cell culture medium according to claim 9, characterized in that: The edge shape of the lower support plate (501) matches the shape of the slot (601), and a number of the fixing clips (504) are arranged in a ring array on the top of the lower support plate (501) and the upper support plate (503).

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