Sample preparation device for quantitative detection and application thereof

Abstract

The invention discloses a sample preparation device for quantitative detection and application of the sample preparation device. The sample preparation device for quantitative detection comprises a quantitative bottle and a sealing assembly, the quantitative bottle comprises a bottle opening and a bottle body; the sealing assembly comprises a connector, a main body and a liquid outlet pipe; the connector, the main body and the liquid outlet pipe are integrally formed; the connecting opening is detachably connected with the bottle opening in a sealing manner; an air vent is formed in the main body, a constant-pressure pipe is arranged in the direction from the air vent to the connecting port in an extending mode, and the constant-pressure pipe penetrates through the connecting port; the sealing assembly further comprises a sealing piece used for sealing the ventilation opening. And a liquid outlet valve is arranged on the liquid outlet pipe. The sample preparation device for quantitative detection has the effects of quantifying the volume of the solution, the reaction bottle and the separating funnel, and can be suitable for preparing various to-be-detected solutions for quantitative detection. The accuracy and precision of the test result are ensured, the preparation steps of the to-be-detected liquid are simplified, and the detection cost is reduced.

Description

Technical Field

[0001] This invention relates to the field of laboratory instruments and equipment, and in particular to a sample preparation device for quantitative detection and its application. Background Technology

[0002] Currently, in the field of quantitative compound analysis, the analyte may require the sequential addition of multiple solvents during the quantitative testing process. Each solvent needs to be added sequentially using a pipette or similar device to achieve dissolution or complete a trace reaction (derivatization). Because the total volume of the mixed solvents is slightly less than the sum of their individual volumes, this can lead to inaccurate solute concentration results and poor precision. Furthermore, pipettes or tubes require periodic calibration and multiple rinses, making the operation relatively cumbersome and increasing equipment costs. For example, if a compound has no response or a low response value in the detection method, a derivatization-extraction method is needed to prepare the analyte. In this process, different reagents need to be added in small amounts and multiple times to complete the derivatization process. Multiple reagent additions can easily introduce deviations in the total volume, thus affecting the detection results. Figure 1 While the traditional stoppered graduated cylinder shown can solve the aforementioned problem of large error, when dealing with experiments that require extraction and where the test solution is a lower layer solution, one method is to use a pipette to directly draw the lower layer solution, which will pass through the upper layer solution during the drawing process, posing a risk of contamination; another method requires the use of a separatory funnel to obtain the lower layer solution, which results in solution loss during the transfer process. This not only further increases the detection error and makes the operation more cumbersome, but also increases the procurement cost.

[0003] Therefore, there is an urgent need in this field for a sample preparation device for the test liquid that is suitable for different types of quantitative detection, and that not only has ideal detection accuracy and precision, but is also easy to operate and reduces detection costs. Summary of the Invention

[0004] The technical problem this application aims to solve is to overcome the shortcomings of existing devices in preparing quantitative detection test solutions, such as large errors, poor precision, and the need for multiple devices to complete the test solution preparation. For example, for detections requiring extraction where the test solution is in the lower layer, at least a pipette or pipette tube, reaction flask, separating funnel, and glass rod are needed, which not only increases the cost of equipment but also makes the operation cumbersome, time-consuming, and labor-intensive. Based on the above-mentioned shortcomings of existing equipment, this invention provides a sample preparation device for quantitative detection and its application. This sample preparation device for quantitative detection combines the functions of a quantitative solution volume, a reaction flask, and a separating funnel, and is applicable to the preparation of test solutions for various quantitative detections. It not only ensures the accuracy and precision of test results but also simplifies the test solution preparation steps and reduces detection costs.

[0005] This application solves the above-mentioned technical problems through the following technical solutions.

[0006] This application provides a sample preparation device for quantitative detection, including a quantitative bottle and a sealing assembly; the quantitative bottle includes a bottle mouth and a bottle body; the sealing assembly includes a connecting port, a main body, and a liquid outlet tube; the connecting port, the main body, and the liquid outlet tube are integrally formed; the connecting port and the bottle mouth are detachably and sealed together; the main body is provided with a vent, and a constant pressure tube extends from the vent towards the connecting port, the constant pressure tube passing through the connecting port; the sealing assembly also includes a sealing element for sealing the vent; a liquid outlet valve is provided on the liquid outlet tube.

[0007] In some embodiments, the bottle body is shaped like a hollow cylinder, such as a hollow cylinder or a hollow cuboid.

[0008] In some embodiments, the bottle body is made of glass or polytetrafluoroethylene.

[0009] In some embodiments, the bottle body is transparent or brown.

[0010] In some embodiments, the bottle is provided with volume markings. As is customary in the art, the lowest volume marking of the sample preparation device for quantitative detection, used to hold the liquid, is "0", with the volume markings gradually increasing from bottom to top.

[0011] In some embodiments, the bottle body is provided with at least one cavity.

[0012] In a preferred embodiment, the cavity is located in the middle or lower part of the bottle body.

[0013] In a preferred embodiment, the cavity is provided with a volume scale. The volume scale value on the cavity represents the volume from the bottom layer of the sample preparation device used to hold the liquid to the volume scale line.

[0014] In a preferred embodiment, the cavity is spherical, ellipsoidal, conical, or pear-shaped.

[0015] In a preferred embodiment, at least one of the cavities is disposed in the middle part of the bottle body, and volume scales are provided on both the bottle body below the cavity and the bottle body above the cavity.

[0016] In some embodiments, a base is provided below the bottle body.

[0017] In a preferred embodiment, the base is circular or square in shape.

[0018] In some embodiments, the sealing assembly is selected from a sealing cap or a sealing plug.

[0019] In some embodiments, the sealing connection between the connector and the bottle opening is selected from screw thread, bayonet, ground joint, or sealing ring.

[0020] In some embodiments, the sample preparation device for quantitative detection further includes an adapter, through which the connection port and the bottle opening are detachably and sealingly connected. When the sizes of the connection port and the bottle opening do not match and they cannot be directly sealed, an indirect sealing connection can be achieved through the adapter.

[0021] In some embodiments, the main body is a cavity structure.

[0022] In some embodiments, the main body is conical, cylindrical, or cylindrical at one end near the connection port and conical at the other end near the outlet pipe.

[0023] In some embodiments, the constant pressure tube is integrally formed with the vent, fixedly connected, or detachably sealed.

[0024] In a preferred embodiment, the vent extends toward the connection port and is provided with a first connecting portion, the first connecting portion being integrally formed with the vent, and the constant pressure tube being detachably and sealed to the first connecting portion.

[0025] In a preferred embodiment, the sealing connection between the constant pressure tube and the first connecting part is a screw thread, a bayonet joint, a ground joint, or a sealing ring.

[0026] In some embodiments, the sealing connection between the seal and the vent is a threaded joint, a bayonet joint, a ground joint, or a sealing ring.

[0027] In some embodiments, the seal is a sealing plug or a sealing cap.

[0028] In a preferred embodiment, the sealing element is a sealing plug, which is inserted into the vent to seal the vent.

[0029] In another preferred embodiment, the vent extends away from the connection port and is provided with a second connecting portion, and the seal is detachably and sealingly connected to the second connecting portion. The second connecting portion communicates with the constant pressure pipe.

[0030] In some embodiments, after the metering bottle and the sealing assembly are assembled, the constant pressure tube extends into the metering bottle.

[0031] In some embodiments, the length of the constant pressure tube can be conventionally selected according to the volume of liquid in the quantitative bottle, ensuring that when the sample preparation device for quantitative detection is inverted, the end of the constant pressure tube near the bottom of the quantitative bottle is above the liquid surface.

[0032] In a preferred embodiment, the constant pressure tube extends to the bottom of the metering bottle.

[0033] In some embodiments, the outlet valve is a plug valve.

[0034] In some embodiments, the end of the outlet tube away from the main body is provided with a drainage bevel.

[0035] In some embodiments, the sample preparation device for quantitative detection further includes a connecting clip for tightly connecting and fixing the connecting port and the bottle opening together.

[0036] This application also provides the application of the sample preparation device for quantitative detection as described above in the preparation of test solutions for the quantitative detection of amino acid compounds.

[0037] In some embodiments, the amino acid compound is selected from at least one of lysine, arginine, histidine, alanine, leucine, tryptophan, threonine, cysteine, tyrosine, aspartic acid, glutamine, and glutamic acid.

[0038] Based on common knowledge in the field, the above-mentioned preferred conditions can be combined arbitrarily to obtain various preferred embodiments of this application.

[0039] All reagents and raw materials used in this application are commercially available.

[0040] The significant advancements of this application are as follows: It provides a sample preparation device for quantitative detection that simultaneously functions as a quantitative solution volume analyzer, a reaction flask, and a separatory funnel, making it suitable for preparing various quantitative detection solutions. This not only ensures the accuracy and precision of the test results but also simplifies the solution preparation process and reduces detection costs. Attached Figure Description

[0041] Figure 1 This is a schematic diagram of an existing stoppered graduated cylinder;

[0042] Figure 2 This is a three-dimensional structural diagram of the sample preparation device for quantitative detection according to Embodiment 1 of the present invention;

[0043] Figure 3 This is a cross-sectional view along the height direction of the sample preparation device for quantitative detection in Embodiment 1 of the present invention;

[0044] Figure 4 This is a three-dimensional structural diagram of the sealing assembly in Embodiment 1 of the present invention;

[0045] Figure 5 This is a schematic diagram of the metering bottle in Embodiment 1 of the present invention;

[0046] Figure 6 This is a cross-sectional view of the sealing assembly along the height direction in an exemplary embodiment of the present invention;

[0047] Figure 7This is a schematic diagram of the structure of a metering bottle in an exemplary embodiment of the present invention;

[0048] Figure 8 This is a schematic diagram of the structure of a metering bottle in an exemplary embodiment of the present invention;

[0049] Figure 9 This is a schematic diagram of the structure of a metering bottle in an exemplary embodiment of the present invention;

[0050] Figure 10 This is a cross-sectional view of the sealing assembly along the height direction in an exemplary embodiment of the present invention.

[0051] Figure Labels

[0052] Quantitative bottle 1, bottle mouth 11, bottle body 12, container cavity 121, volume scale 13, base 14;

[0053] Sealing assembly 2, connection port 21, main body 22, liquid outlet pipe 23, vent port 24, constant pressure pipe 25, sealing element 26, liquid outlet valve 27, first connection part 28, second connection part 29;

[0054] Connector clip 3. Detailed Implementation

[0055] The present application is further illustrated below by way of embodiments, but these embodiments are not intended to limit the scope of the present application. Experimental methods not specifically described in the following embodiments are performed according to conventional methods and conditions, or as selected in accordance with the product instructions.

[0056] In the description of this invention, it should be noted that the terms "upper", "lower", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0057] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the term "connection" should be interpreted broadly. For example, it can refer to a fixed connection or a detachable connection; it can refer to a mechanical connection or an adhesive connection; it can refer to a direct connection or an indirect connection through an intermediate medium; it can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0058] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present invention.

[0059] Example 1

[0060] The following is combined with Figures 2 to 5 The present invention provides a sample preparation device for quantitative detection, which includes a quantitative bottle 1 and a sealing assembly 2, and specific embodiments thereof. The quantitative bottle 1 includes a bottle mouth 11 and a bottle body 12. The sealing assembly 2 includes a connection port 21, a main body 22 and a liquid outlet tube 23. The connection port 21, the main body 22 and the liquid outlet tube 23 are integrally formed. The connection port 21 and the bottle mouth 11 are detachably sealed together. A vent 24 is provided on the main body 22, and a constant pressure tube 25 extends from the vent 24 toward the connection port 21 and passes through the connection port 21. The sealing assembly 2 also includes a sealing element 26 for sealing the vent 24. A liquid outlet valve 27 is provided on the liquid outlet tube 23.

[0061] When performing quantitative analysis of compounds requiring complex pretreatment, the total volume of different solvents during mixing is slightly less than the sum of the volumes of the individual solvents. This can lead to inaccurate solute concentration results and poor precision. Furthermore, pipettes or pipettes require regular calibration and multiple rinses, which is relatively cumbersome and increases equipment costs. For analyses requiring extraction and where the analyte has a high density and is in the lower layer, a separatory funnel is also necessary. Due to solution residue during transfer, this not only increases detection errors but also requires additional equipment such as separatory funnels, increasing costs and adding to the complexity. However, by assembling the quantitative bottle 1 with the aforementioned specific sealing component 2, pretreatment can be performed within the quantitative bottle 1. The amount of sample added is based on the volume scale on the quantitative bottle 1. By aligning with the scale line (viewing at eye level, with the liquid surface tangent to the scale line), the solvent can be added accurately and quantitatively. This design standardizes the sample preparation method, avoids arbitrarily changing the diluent ratio during quantification, and improves the reproducibility of the analysis. By using a sealing assembly 2 with a constant pressure tube 25, the lower layer of the test liquid with high density can be obtained without the aid of a separating funnel. This not only solves the problem of solution loss during liquid transfer, but also simplifies experimental operations and reduces detection costs.

[0062] In this embodiment, the bottle body 12 is a hollow cylinder. In other embodiments, the bottle body 12 may also be a hollow cuboid.

[0063] In this embodiment, a cavity 121 is provided in the middle of the bottle body 12. For cases where dilution or extraction is required after pretreatment before detection, the cavity 121 on the bottle body 12 is provided to expand the volume and reduce the height of the bottle body 12. In addition, the cavity 121 is also conducive to the thorough mixing of the composite solution.

[0064] In other embodiments, the number of cavities 121 may also be multiple, for example, two (see [reference]). Figure 9 (3, 4 or more) . The cavities 121 can also be set in different parts of the bottle body 12 according to actual needs. For example, according to the ratio of each reaction liquid, the ratio of reaction liquid to diluent, or the ratio of reaction liquid to extractant, the appropriate number and volume of cavities 121 can be set in the required positions.

[0065] In this embodiment, volume scales 13 are provided on the cavity 121, the bottle body 12 above the cavity 121, and the bottle body 12 below the cavity 121. The volume scale 13 on the bottom surface of the bottle body 12 has a value of "0" and the volume scale value gradually increases from bottom to top.

[0066] In other embodiments, if the cavity 121 is only used to store liquid and does not need to play a quantitative role, the cavity 121 may not be provided with volume scale 13.

[0067] In this embodiment, the cavity 121 is pear-shaped. In other embodiments, the cavity 121 may also be spherical (see...). Figure 7 ), ellipsoid or cone (see Figure 8 ).

[0068] In this embodiment, a circular base 14 is provided below the bottle body 12, and the diameter of the base 14 is larger than the maximum diameter of the transverse cross section of the cavity 121. The shape and size of the base 14 can be selected according to the actual situation to ensure that the sample preparation device for quantitative detection can stand stably on the operating table during use and is not easy to tip over.

[0069] In other embodiments, if the bottom of the bottle body 12 is provided with a cavity 121, and the area of ​​the lower surface of the cavity 121 is large enough to serve as a base 14, then there is no need to provide an additional base 14 (see [reference]). Figure 9 At this point, the volume scale value on the bottom surface of the cavity 121 is "0", and the volume scale value gradually increases from bottom to top.

[0070] In this embodiment, the sealing component 2 is a sealing cap. In other embodiments, the sealing component 2 may also be a sealing plug, which is inserted into the mouth 11 of the metering bottle 1.

[0071] In this embodiment, the inner side of the connection port 21 of the sealing component 2 is a ground joint, and the outer side of the bottle mouth 11 of the quantitative bottle 1 is a ground joint. The connection port 21 is fitted over the outer side of the bottle mouth 11, and the two are sealed together. This structure provides a better sealing effect, and the liquid is less likely to leak out when the sample preparation device for quantitative detection is inverted. In other embodiments, the bottle mouth 11 can be fitted over the outer side of the connection port 21. In other embodiments, the connection port 21 and the bottle mouth 11 can also be sealed together by means of a screw thread, a bayonet, or a sealing ring.

[0072] In this embodiment, the connection port 21 of the sealing component 2 and the bottle mouth 11 of the metering bottle 1 are directly sealed together. In other embodiments, when the size of the connection port 21 of the sealing component 2 and the bottle mouth 11 of the metering bottle 1 do not match and cannot be directly sealed together, the connection port 21 and the bottle mouth 11 can be sealed together by an adapter.

[0073] In this embodiment, the main body 22 has a hollow structure, with one end near the connection port 21 being cylindrical and the other end near the liquid outlet pipe 23 being conical. In other embodiments, the main body 22 may also be conical or cylindrical.

[0074] In this embodiment, the constant pressure tube 25 and the vent 24 are detachably and sealingly connected. In other embodiments, the constant pressure tube 25 and the vent 24 can be integrally formed (see...). Figure 6 (or fixed connection)

[0075] In this embodiment, a first connecting part 28 is provided extending from the vent 24 toward the connecting port 21. The first connecting part 28 is integrally formed with the vent 24, and the constant pressure tube 25 is detachably and sealed to the first connecting part 28.

[0076] In this embodiment, the constant pressure tube 25 and the first connecting part 28 are connected by a threaded seal. In other embodiments, the constant pressure tube 25 and the first connecting part 28 can also be connected by a bayonet, a ground joint, or a sealing ring.

[0077] In this embodiment, the sealing element 26 is a sealing plug, inserted into the vent 24 to seal the vent 24. The sealing element 26 and the vent 24 are connected by a ground joint. In other embodiments, the sealing element 26 and the vent 24 can be connected by a screw thread, a bayonet, or a sealing ring. For example, in one embodiment (see...) Figure 10 The sealing element 26 is a sealing cap. A second connecting portion 29 extends from the vent 24 away from the connection port 21. The second connecting portion 29 is integrally formed with the vent 24 and communicates with the constant pressure tube 25. The sealing element 26 and the second connecting portion 29 are connected by a screw thread. In this embodiment, the sealing effect of the sealing element 26 is more ideal, and the sealing element 26 is less likely to fall off when the quantitative detection sample preparation device is inverted, making it more convenient to use.

[0078] In this embodiment, after the quantitative bottle 1 and the sealing assembly 2 are assembled, the constant pressure tube 25 extends into the quantitative bottle 1 and reaches the bottom of the bottle. In other embodiments, the length of the constant pressure tube 25 can be conventionally selected according to the volume of liquid in the quantitative bottle 1, ensuring that when the sample preparation device for quantitative detection is inverted, the end of the constant pressure tube 25 near the bottom of the quantitative bottle 1 is above the liquid surface.

[0079] In this embodiment, the liquid outlet valve 27 is a plug valve, which has a channel for liquid flow. Figure 3 This is a diagram showing the state of the plug valve when it is open. When the plug is open, the channel for liquid flow is connected to the outlet pipe 23.

[0080] In this embodiment, the end of the liquid outlet pipe 23 away from the main body 22 is provided with a drainage angle.

[0081] In this embodiment, the sample preparation device for quantitative detection also includes a connecting clip 3, which is used to tightly connect and fix the connecting port 21 and the bottle mouth 11 together. The connecting clip 3 ensures a tighter connection between the connecting port 21 and the bottle mouth 11, preventing leakage; on the other hand, it prevents the sealing component 2 from falling off when the sample preparation device for quantitative detection is inverted, making it safer and more convenient to use.

[0082] When using the sample preparation device for quantitative detection of the present invention, the required volume of reaction liquid is added to the quantitative bottle 1 according to the volume scale 13 on the quantitative bottle 1. After adding, the sealing member 26 is sealed to the vent 24, and the connection port 21 on the sealing assembly 2 is sealed to the bottle mouth 11 on the quantitative bottle 1. At this time, the constant pressure tube 25 can be removed, and the mixture is shaken to carry out the mixing reaction. If gas is generated or heat is released during the mixing reaction, the liquid outlet valve 27 can be opened to discharge the gas from the liquid outlet pipe 23 or to balance the internal and external pressure. For sample preparation processes that require dilution or extraction, the sealing assembly 2 is removed after the reaction, and diluent or extractant is added to the quantitative bottle 1. The amount to be added can be determined according to the volume scale 13 on the bottle body 12 or the cavity 121. To obtain the lower layer extract, install the constant pressure tube 25, ensuring a sealed connection between the constant pressure tube 25 and the first connection part 28. Simultaneously, ensure that the sealing element 26 is sealed to the vent 24 to prevent liquid from entering the constant pressure tube 25 when it is inside the quantitative bottle 1. After installing the constant pressure tube 25, seal the connection port 21 on the sealing component 2 and the bottle mouth 11 on the quantitative bottle 1. After shaking and extraction, install the connecting clamp 3 and invert the quantitative detection sample preparation device. During inversion, the end of the constant pressure tube 25 closest to the bottom of the quantitative bottle 1 should be quickly positioned above the liquid surface to prevent liquid from entering the constant pressure tube 25. After inversion, remove the sealing element 26 to ensure equal pressure inside and outside the device. After separation, the test liquid is released from the outlet tube 23.

[0083] This novel quantitative detection sample preparation device can also handle detections that do not require extraction or where the sample solution is located on the upper layer after extraction. During use, the constant pressure tube 25 is not required. After mixing and reacting, the sealing component 2 can be opened, and the upper sample solution can be directly aspirated with a dropper.

[0084] Example 2

[0085] Most amino acids show no significant absorption or only weak absorption under a UV detector. Therefore, pre-column derivatization is necessary in quantitative analysis to improve detection sensitivity. Reagents such as phenyl isothiocyanate are commonly used to derivatize amino acids before content determination. This example uses the sample preparation device for quantitative detection described in Example 1 to prepare the sample. The maximum volume graduation of the sample preparation device for quantitative detection is 10 mL. The specific steps include:

[0086] Derivatization reaction: Add the amino acid sample solution to the first graduation mark (0.5 mL) of the quantitative bottle 1 using a dropper, making the concave meniscus tangent to the solvent graduation mark; in other embodiments, the amino acid sample can also be accurately weighed, placed at the bottom of the quantitative bottle 1, dissolved and diluted with water to the first graduation mark. Then add 0.1 mol / L hydrochloric acid to the second graduation mark (1.0 mL) using a dropper, mix well, then add 14% triethylamine acetonitrile solution to the third graduation mark (1.5 mL) using a dropper, and finally add 0.1 mol / L phenyl isothiocyanate acetonitrile to the fourth graduation mark (2.0 mL). Seal the sealing component 26 to the vent 24, and seal the connection port 21 on the sealing component 2 to the bottle mouth 11 on the quantitative bottle 1. At this time, the constant pressure tube 25 is not installed. Mix well to complete the derivatization process.

[0087] Extraction: After the derivatization reaction, remove the sealing assembly 2, add 1.5 mL of n-hexane to the quantitative bottle 1, vortex for 30 seconds, let stand, and then dilute with water to bring the interface to the full mark (10 mL). Install the constant pressure tube 25 on the sealing assembly 2, ensuring that the constant pressure tube 25 is sealed to the first connection part 28, and also ensuring that the seal 26 is sealed to the vent 24 to prevent liquid from entering the constant pressure tube 25 when it is in the quantitative bottle 1. After the constant pressure tube 25 is installed, seal the connection port 21 on the sealing assembly 2 and the bottle mouth 11 on the quantitative bottle 1. After shaking and extraction, install the connection clip 3, and invert the sample preparation device for quantitative detection. During the inversion, the end of the constant pressure tube 25 closest to the bottom of the quantitative bottle 1 should be quickly positioned above the liquid surface to prevent liquid from entering the constant pressure tube 25. After inversion, remove the seal 26 to make the pressure inside and outside the device the same. After the separation is completed, the test liquid is released from the outlet tube 23 for quantitative detection.

[0088] Although this application has been disclosed above through the description of specific embodiments, it should be understood that those skilled in the art can devise various modifications, improvements, or equivalents to this application within the spirit and scope of the appended solutions. Such modifications, improvements, or equivalents should also be considered to be included within the scope of protection claimed in this application.

Claims

1. A sample preparation device for quantitative detection, characterized in that, The device includes a metering bottle and a sealing assembly; the metering bottle includes a bottle mouth and a bottle body; the sealing assembly includes a connecting port, a main body, and a dispensing tube; the connecting port, the main body, and the dispensing tube are integrally formed; the connecting port and the bottle mouth are detachably and sealed together; the main body is provided with a vent, and a constant pressure tube extends from the vent towards the connecting port, the constant pressure tube passing through the connecting port; the sealing assembly also includes a sealing element for sealing the vent; and a dispensing valve is provided on the dispensing tube.

2. The sample preparation device for quantitative detection as described in claim 1, characterized in that, The sample preparation device for quantitative detection satisfies at least one of the following conditions (1) to (6): (1) The shape of the bottle body is a hollow cylinder; (2) The bottle body is made of glass or polytetrafluoroethylene; (3) The bottle body is transparent or brown in color; (4) The bottle body is provided with volume scale; (5) At least one cavity is provided on the bottle body; (6) A base is provided at the bottom of the bottle.

3. The sample preparation device for quantitative detection as described in claim 2, characterized in that, The sample preparation device for quantitative detection satisfies at least one of the following conditions (1) to (5): (1) The shape of the bottle body is a hollow cylinder or a hollow cuboid; (2) The cavity is located in the middle or lower part of the bottle body; (3) The cavity is provided with a volume scale; (4) The shape of the cavity is spherical, ellipsoidal, conical or pear-shaped; (5) The base is round or square in shape.

4. The sample preparation device for quantitative detection as described in claim 3, characterized in that, At least one of the cavities is located in the middle of the bottle body, and volume markings are provided on both the bottle body below and above the cavity.

5. The sample preparation device for quantitative detection as described in claim 1, characterized in that, The sample preparation device for quantitative detection satisfies at least one of the following conditions (1) to (8): (1) The sealing component is selected from a sealing cap or a sealing plug; (2) The sealing connection method between the connection port and the bottle mouth is selected from a screw port, a bayonet port, a ground joint, or a sealing ring; (3) The sample preparation device for quantitative detection also includes an adapter, and the connection port and the bottle mouth are detachably and sealed together by the adapter; (4) The main body is a hollow structure; (5) The main body is conical, cylindrical, or has a cylindrical end near the connection port and a conical end near the liquid outlet pipe; (6) The constant pressure tube and the vent are integrally formed, fixedly connected, or detachably sealed; (7) The sealing connection between the sealing element and the vent is a threaded joint, a bayonet joint, a ground joint, or a sealing ring; (8) The sealing element is a sealing plug or a sealing cap.

6. The sample preparation device for quantitative detection as described in claim 5, characterized in that, The vent extends toward the connection port and is provided with a first connecting part, which is integrally formed with the vent. The constant pressure tube is detachably and sealed to the first connecting part.

7. The sample preparation device for quantitative detection as described in claim 6, characterized in that, The sealing connection between the constant pressure tube and the first connecting part is a screw thread, a bayonet joint, a ground joint, or a sealing ring.

8. The sample preparation device for quantitative detection according to claim 5, characterized in that, The vent extends away from the connection port and is provided with a second connection part. The seal is detachably and sealingly connected to the second connection part, and the second connection part is connected to the constant pressure pipe.

9. The sample preparation apparatus for quantitative detection according to any one of claims 1 to 8, characterized in that, The sample preparation device for quantitative detection satisfies at least one of the following conditions (1) to (4): (1) After the metering bottle and the sealing assembly are assembled, the constant pressure tube extends into the metering bottle; preferably, the constant pressure tube extends to the bottom of the metering bottle; (2) The outlet valve is a plug valve; (3) The end of the outlet pipe away from the main body is provided with a flow-guiding angle; (4) The sample preparation device for quantitative detection also includes a connecting clamp for tightly connecting and fixing the connecting port and the bottle mouth together.

10. The application of the sample preparation device for quantitative detection as described in any one of claims 1 to 9 in the preparation of test solutions for quantitative detection of amino acid compounds.

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