An enzyme moisturizing composition, its preparation method and use
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- GUANGDONG QIAOQIAO BIOTECHNOLOGY CO LTD
- Filing Date
- 2025-09-30
- Publication Date
- 2026-06-02
Abstract
Description
Technical Field
[0001] This invention belongs to the field of cosmetic technology and relates to an enzyme moisturizing composition, its preparation method, and its application. Background Technology
[0002] In the field of skincare, face masks are widely used in daily skincare routines as a highly effective moisturizing method. They work by creating a sealed environment on the skin's surface to promote the penetration of active ingredients, achieving deep hydration and repair. However, in actual use, if the cleansing step is not performed adequately, such as using only warm water or using a cleanser with insufficient cleansing power, a large amount of oil will remain on the skin's surface. This oil forms a physical barrier between the mask ingredients and the skin, significantly hindering the penetration of moisturizing active ingredients into the deeper layers of the skin. At the same time, it inhibits the skin's ability to absorb moisture, resulting in a significant reduction in the moisturizing effect of the mask.
[0003] It is worth noting that enzyme-based ingredients, such as yeast / rice ferment filtrate, are rich in bioactive substances such as proteins, peptides, and organic acids. They exhibit excellent moisturizing, barrier repair, and metabolism-boosting potential in face masks, making them a core functional ingredient in modern high-efficiency moisturizing masks. However, existing enzyme-containing face mask products cannot effectively overcome the barrier of residual oil, preventing the enzymes from fully contacting the skin. Consequently, their bioactivity and moisturizing effects cannot be fully realized, severely impacting the product's effectiveness. Summary of the Invention
[0004] The purpose of this invention is to provide an enzyme moisturizing composition, its preparation method and application, which can overcome the barrier of residual oil on the skin to fully exert the moisturizing effect of yeast / rice fermentation product filtrate.
[0005] The objective of this invention can be achieved through the following technical solutions:
[0006] In a first aspect, the present invention provides an enzyme moisturizing composition comprising the following components: yeast / rice fermentation product filtrate, an amphiphilic complex, sodium hyaluronate, glycerin, panthenol, propylene glycol, sodium benzoate, and purified water.
[0007] The amphiphilic complex consists of phosphatidylcholine, PEG-10 stearate, and lipase.
[0008] Preferably, the mass ratio of phosphatidylcholine, PEG-10 stearate, and lipase is (0.3-0.5):(0.3-0.5):(0.2-0.3).
[0009] Preferably, the components include the following parts by weight: 55-65 parts yeast / rice fermentation product filtrate, 0.8-1.5 parts amphiphilic complex, 0.5-1.0 parts sodium hyaluronate, 6-8 parts glycerol, 1.5-2.5 parts panthenol, 3-5 parts propylene glycol, 0.2-0.4 parts sodium benzoate, and 16.6-33 parts purified water.
[0010] Preferably, the method for preparing the amphiphilic complex includes the following steps:
[0011] S1. Dissolve phosphatidylcholine and PEG-10 stearate in an aqueous ethanol solution to obtain mixture a;
[0012] S2. Adjust the temperature of mixture a to 0-4℃, add lipase, stir well to obtain mixture b;
[0013] S3. After filtering the mixed liquid b through a microporous membrane, it is ultrasonically treated to obtain the amphiphilic complex.
[0014] Preferably, the pore size of the microporous filter membrane in step S3 is 0.3-0.4 μm.
[0015] In a second aspect, the present invention provides a method for preparing an enzyme moisturizing composition as described in the first aspect, comprising the following steps:
[0016] Y1. Mix the yeast / rice fermentation product filtrate with purified water and stir at room temperature until homogeneous to obtain mixture A;
[0017] Y2. Add sodium hyaluronate, glycerin, panthenol and propylene glycol to mixture A in sequence, and stir at 25-30℃ until completely dissolved to obtain mixture B;
[0018] Y3. Add the amphiphilic complex to the mixture B, stir evenly, adjust the pH to 5.5-6.0, then add sodium benzoate and stir until uniform to obtain the enzyme moisturizing composition.
[0019] Thirdly, the present invention provides a face mask comprising a face mask substrate, an enzyme moisturizing composition as described in the first aspect, and an auxiliary moisturizing composition, wherein the side of the face mask substrate that contacts the skin is designated as side a, and the side of the face mask substrate that does not contact the skin is designated as side b, the enzyme moisturizing composition is coated on side a, and the auxiliary moisturizing composition is coated on side b.
[0020] Preferably, the auxiliary moisturizing composition comprises the following components in parts by weight: 2-3 parts trehalose, 0.5-1.0 parts sodium hyaluronate, and 6-8 parts purified water.
[0021] Preferably, the mask substrate is a cellulose membrane, and the thickness of the mask substrate is 0.05-0.1 mm.
[0022] Fourthly, the present invention provides a method for preparing a facial mask as described in the third aspect, comprising the following steps: first, coating an enzyme moisturizing composition onto side a of a facial mask substrate, and drying it until the moisture content of side a is 20-25%; then, coating an auxiliary moisturizing composition onto side b of the facial mask substrate, and drying it until the moisture content of side b is 20-25%.
[0023] The beneficial effects of this invention are:
[0024] (1) This invention utilizes a specific amphiphilic complex (phosphatidylcholine, PEG-10 stearate, and lipase in a specific ratio) to actively bind residual oil on the skin surface. The amphiphilic structure of phosphatidylcholine and PEG-10 stearate can quickly adsorb oil, and lipase assists in the dispersion of oil to form a uniform emulsion system. Combined with the innovative layered design of the mask's a side (high lipophilicity) and b side (high hydrophilicity), the oil spontaneously completes directional migration under the drive of the affinity gradient. The a side constructs an lipophilic interface through phosphatidylcholine and PEG-10 stearate to efficiently capture skin oil; the b side utilizes the high hydrophilicity of trehalose and sodium hyaluronate to form a strongly hygroscopic environment, generating an affinity potential difference from the a side to the b side. This gradient-driven mechanism allows oil to continuously migrate from the lipophilic a side to the hydrophilic b side, forming a migration channel. This eliminates the oil barrier layer between the skin and the a side, ensuring that the active enzyme ingredients such as proteins and peptides in the yeast / rice fermentation product filtrate can directly contact the skin. This addresses the problem of limited efficacy of enzyme-containing masks due to oil residue at the root, significantly improving moisturizing longevity and barrier repair effects.
[0025] (2) The yeast / rice fermentation product filtrate in the composition serves as the core enzyme source, which can replenish the active substances needed by the skin and promote metabolism; sodium hyaluronate, glycerin, and panthenol form a multi-layer moisturizing system; sodium hyaluronate locks in water, glycerin retains moisture, and panthenol repairs the skin barrier. The three, together with the enzyme, enhance the lasting moisturizing effect; propylene glycol not only assists in moisturizing but also promotes the penetration of various active ingredients into the skin; sodium benzoate ensures the stability of the composition during long-term storage.
[0026] (3) The mask adopts a layered structure of “a side (enzyme and amphiphilic complex) - substrate - b side (auxiliary moisturizing composition)”. The a side focuses on oil binding and enzyme release, while the b side uses a combination of trehalose and sodium hyaluronate to hold the oil and replenish moisture, preventing oil backflow. At the same time, the water content of each composition is coated in stages and controlled, so that the two compositions form a stable physical barrier, preventing the ingredients from penetrating and mixing during storage, and ensuring that each functional layer plays its role precisely when the mask is used. In addition, the thickness of the cellulose substrate is controlled at 0.05-0.1mm, which has both high water absorption and adhesion, improving the skin feel. Detailed Implementation
[0027] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the following detailed description of the specific implementation methods, structures, features, and effects of the present invention, in conjunction with embodiments, is provided below.
[0028] The following descriptions of some of the raw materials used in the examples and comparative examples are as follows:
[0029] Yeast / rice fermentation product filtrate was purchased from Jining Fangyu Chemical Co., Ltd.
[0030] PEG-10 stearate was purchased from Jiangsu Leien Environmental Protection Technology Co., Ltd.
[0031] The lipase was purchased from Sichuan Huanxu Biotechnology Co., Ltd.
[0032] Sodium hyaluronate was purchased from Xi'an Aosai Biotechnology Co., Ltd.
[0033] Trehalose was purchased from Zhejiang Tengrui Biotechnology Co., Ltd.
[0034] Example 1
[0035] An enzyme moisturizing composition comprising the following components in parts by weight: 60 parts yeast / rice fermentation product filtrate, 1 part amphiphilic complex, 0.7 parts sodium hyaluronate, 7 parts glycerin, 2 parts panthenol, 4 parts propylene glycol, 0.3 parts sodium benzoate, and 25 parts purified water.
[0036] The amphiphilic complex consists of phosphatidylcholine, PEG-10 stearate, and lipase. The mass ratio of phosphatidylcholine, PEG-10 stearate, and lipase is 0.4:0.4:0.2.
[0037] The preparation method of the amphiphilic complex specifically includes the following steps:
[0038] S1. Add 0.4 parts of phosphatidylcholine and 0.4 parts of PEG-10 stearate to 100 mL of 80% ethanol aqueous solution, and stir for 30 min at 4℃ and 300 rpm until phosphatidylcholine and PEG-10 stearate are completely dissolved to obtain mixture a.
[0039] S2. Using an ice bath, control the temperature of mixture a to 2℃. Add 0.2 parts of lipase to mixture a, maintain the temperature at 2℃, and continue stirring at 300 rpm for 20 minutes to ensure that the lipase is evenly dispersed in the mixture, thus obtaining mixture b.
[0040] S3. The mixture b is filtered through a 0.35μm microporous membrane to remove any undissolved particulate impurities that may be present in the mixture. The filtered filtrate is then placed in an ultrasonic device and ultrasonically treated at 50W power for 10 minutes to obtain the amphiphilic complex.
[0041] A method for preparing an enzyme moisturizing composition specifically includes the following steps:
[0042] Y1. Add 60 parts of yeast / rice fermentation product filtrate and 25 parts of purified water to a stirring container and stir for 5 minutes at room temperature (25°C) and speed (300 rpm) to ensure that the yeast / rice fermentation product filtrate and purified water are fully mixed to obtain mixture A.
[0043] Y2. Add 0.7 parts sodium hyaluronate, 7 parts glycerin, 2 parts panthenol, and 4 parts propylene glycol to mixture A in sequence. Adjust the temperature in the stirring container to 28°C, increase the speed to 400 rpm, and stir continuously for 20 minutes until sodium hyaluronate, glycerin, panthenol, and propylene glycol are completely dissolved and there are no visible flocculents or stratification in the system, thus obtaining mixture B.
[0044] Y3. Add 1 part of the prepared amphiphilic complex to mixture B, adjust the speed to 250 rpm, and simultaneously purge nitrogen into the stirring container for protection (to prevent the lipase in the amphiphilic complex from being oxidized and deactivated). Stir for 12 min to fully integrate the amphiphilic complex with mixture B. Then adjust the pH of the system to 5.7 with 10% citric acid aqueous solution, add 0.3 parts of sodium benzoate, and continue stirring at 250 rpm for 6 min until the sodium benzoate is completely dissolved and the system is homogeneous, thus obtaining the enzyme moisturizing composition.
[0045] Example 2
[0046] An enzyme moisturizing composition comprising the following components in parts by weight: 55 parts yeast / rice fermentation product filtrate, 1.5 parts amphiphilic complex, 0.5 parts sodium hyaluronate, 6 parts glycerin, 1.5 parts panthenol, 3 parts propylene glycol, 0.2 parts sodium benzoate, and 32.3 parts purified water.
[0047] The amphiphilic complex consists of phosphatidylcholine, PEG-10 stearate, and lipase. The mass ratio of phosphatidylcholine, PEG-10 stearate, and lipase is 0.3:0.5:0.2.
[0048] The preparation method of the amphiphilic complex specifically includes the following steps:
[0049] S1. Add 0.45 parts of phosphatidylcholine and 0.75 parts of PEG-10 stearate to 100 mL of 80% ethanol aqueous solution, and stir for 30 min at 4℃ and 300 rpm until phosphatidylcholine and PEG-10 stearate are completely dissolved to obtain mixture a.
[0050] S2. Using an ice bath, control the temperature of mixture a to 4℃. Add 0.3 parts of lipase to mixture a, maintain the temperature at 4℃ and the stirring speed at 300 rpm for 20 minutes to ensure that the lipase is evenly dispersed in the mixture, thus obtaining mixture b.
[0051] S3. The mixture b is filtered through a 0.4μm microporous membrane to remove any undissolved particulate impurities that may be present in the mixture. The filtered filtrate is then placed in an ultrasonic device and ultrasonically treated at 50W power for 10 minutes to obtain the amphiphilic complex.
[0052] A method for preparing an enzyme moisturizing composition specifically includes the following steps:
[0053] Y1. Add 55 parts of yeast / rice fermentation product filtrate and 32.3 parts of purified water to a stirring container and stir for 5 minutes at room temperature (25°C) and speed (300 rpm) to ensure that the yeast / rice fermentation product filtrate and purified water are fully mixed to obtain mixture A.
[0054] Y2. Add 0.5 parts sodium hyaluronate, 6 parts glycerin, 1.5 parts panthenol, and 3 parts propylene glycol to mixture A in sequence. Adjust the temperature in the stirring container to 25°C, increase the speed to 350 rpm, and stir continuously for 25 minutes until sodium hyaluronate, glycerin, panthenol, and propylene glycol are completely dissolved and there are no visible flocculents or stratification in the system, thus obtaining mixture B.
[0055] Y3. Add 1.5 parts of the prepared amphiphilic complex to mixture B, adjust the stirring speed to 200 rpm, and simultaneously purge nitrogen into the stirring container for protection (to prevent the lipase in the amphiphilic complex from being oxidized and deactivated). Stir for 15 min to fully integrate the amphiphilic complex with mixture B. Then adjust the pH of the system to 5.5 with 10% citric acid aqueous solution, add 0.2 parts of sodium benzoate, and continue stirring at 200 rpm for 8 min until the sodium benzoate is completely dissolved and the system is homogeneous, thus obtaining the enzyme moisturizing composition.
[0056] Example 3
[0057] An enzyme moisturizing composition comprising the following components in parts by weight: 65 parts yeast / rice fermentation product filtrate, 0.8 parts amphiphilic complex, 1.0 part sodium hyaluronate, 8 parts glycerin, 2.5 parts panthenol, 5 parts propylene glycol, 0.4 parts sodium benzoate, and 17.3 parts purified water.
[0058] The amphiphilic complex consists of phosphatidylcholine, PEG-10 stearate, and lipase. The mass ratio of phosphatidylcholine, PEG-10 stearate, and lipase is 0.5:0.3:0.3.
[0059] The preparation method of the amphiphilic complex specifically includes the following steps:
[0060] S1. Add 0.36 parts of phosphatidylcholine and 0.22 parts of PEG-10 stearate to 100 mL of 80% ethanol aqueous solution, and stir for 30 min at 4℃ and 300 rpm until phosphatidylcholine and PEG-10 stearate are completely dissolved to obtain mixture a.
[0061] S2. Using an ice bath, control the temperature of mixture a to 0℃. Add 0.22 parts of lipase to mixture a, maintain the temperature at 0℃ and stir at 300 rpm for 20 minutes to ensure that the lipase is evenly dispersed in the mixture, thus obtaining mixture b.
[0062] S3. The mixture b is filtered through a 0.3μm microporous membrane to remove any undissolved particulate impurities that may be present in the mixture. The filtered filtrate is then placed in an ultrasonic device and ultrasonically treated at 50W power for 10 minutes to obtain the amphiphilic complex.
[0063] A method for preparing an enzyme moisturizing composition specifically includes the following steps:
[0064] Y1. Add 65 parts of yeast / rice fermentation product filtrate and 17.3 parts of purified water to a stirring container and stir for 5 minutes at room temperature (25°C) and speed (300 rpm) to ensure that the yeast / rice fermentation product filtrate and purified water are fully mixed to obtain mixture A.
[0065] Y2. Add 1.0 part sodium hyaluronate, 8 parts glycerin, 2.5 parts panthenol, and 5 parts propylene glycol to mixture A in sequence. Adjust the temperature in the stirring container to 30°C, increase the speed to 500 rpm, and stir continuously for 15 minutes until sodium hyaluronate, glycerin, panthenol, and propylene glycol are completely dissolved and there are no visible flocculents or stratification in the system, thus obtaining mixture B.
[0066] Y3. Add 0.8 parts of the prepared amphiphilic complex to mixture B, adjust the stirring speed to 300 rpm, and simultaneously purge nitrogen into the stirring container for protection (to prevent the lipase in the amphiphilic complex from being oxidized and deactivated). Stir for 10 min to fully integrate the amphiphilic complex with mixture B. Then adjust the pH of the system to 6.0 with 10% citric acid aqueous solution, add 0.4 parts of sodium benzoate, and continue stirring at 300 rpm for 5 min until the sodium benzoate is completely dissolved and the system is homogeneous, thus obtaining the enzyme moisturizing composition.
[0067] Comparative Example 1
[0068] The difference from Example 1 is that the amphiphilic complex is replaced with an equal amount of purified water in the components of the enzyme moisturizing composition.
[0069] Comparative Example 2
[0070] The difference from Example 1 is that 0.2 parts of lipase were removed from the composition of the amphiphilic complex, and the mass ratio of phosphatidylcholine to PEG-10 stearate was adjusted to 0.5:0.5.
[0071] Application Example 1
[0072] A face mask includes a face mask substrate, an enzyme moisturizing composition prepared in Example 1, and an auxiliary moisturizing composition.
[0073] The auxiliary moisturizing composition includes the following components in parts by weight: 2.5 parts trehalose, 0.7 parts sodium hyaluronate, and 7 parts purified water.
[0074] The preparation method of the auxiliary moisturizing composition is as follows: take 2.5 parts by weight of trehalose, 0.7 parts by weight of sodium hyaluronate and 7 parts by weight of purified water, mix and stir at room temperature (25°C) and 200 rpm until completely dissolved, and set aside.
[0075] The side of the mask substrate that contacts the skin is designated as side a, and the side that does not contact the skin is designated as side b. An enzyme moisturizing composition is applied to side a, and an auxiliary moisturizing composition is applied to side b. The mask substrate is made of cellulose membrane with a thickness of 0.08 mm.
[0076] The specific method for preparing a facial mask is as follows:
[0077] Z1. Cut the cellulose membrane to the size required for face fitting (21cm long × 15cm wide, with two 2cm diameter eye holes and one 3cm long × 1cm wide opening). Gently wipe the surface of the membrane with purified water to remove dust, and allow it to air dry naturally at room temperature (25℃).
[0078] Z2. Take the enzyme moisturizing composition prepared in Example 1 and apply it evenly to side a of the cellulose membrane at a coating amount of 18 g / m² using a scraper coating machine (coating speed 2 m / min, ensuring no bubbles or missed coatings in the coating); then place the membrane material in a constant temperature and humidity chamber, set the temperature to 28℃ and the relative humidity to 45%, and dry for 15 min. Use a rapid moisture meter to detect the moisture content of the functional layer on side a until it reaches 23%, then remove it for later use.
[0079] Z3. Under the same constant temperature and humidity chamber conditions, take the above-mentioned auxiliary moisturizing composition and apply it to the b side of the film material at a coating amount of 12g / m² (the coating direction is perpendicular to the a side to avoid coating overlap); continue drying for 12min, and check the moisture content of the functional layer on the b side until it reaches 22%. Remove and cool to room temperature of 25℃.
[0080] Z4. Cut the dried mask into its final usable size (20cm long × 14cm wide) and package it individually using an aluminum-plastic composite film (45μm thick inner PE film and 10μm thick outer aluminum foil). During packaging, nitrogen with a purity of ≥99.99% is introduced through a nitrogen replacement device, and the oxygen residue inside the packaging is detected using a headspace gas analyzer to ensure it is ≤0.5%. After sealing, the mask is sterilized by irradiation with 254nm ultraviolet light for 30s to obtain the finished enzyme moisturizing mask. Store it away from light at a temperature below 25℃.
[0081] Application Example 2
[0082] A face mask includes a face mask substrate, an enzyme moisturizing composition prepared in Example 2, and an auxiliary moisturizing composition.
[0083] The auxiliary moisturizing composition comprises the following components in parts by weight: 2 parts trehalose, 0.5 parts sodium hyaluronate, and 6 parts purified water. The auxiliary moisturizing composition is prepared by mixing trehalose, sodium hyaluronate, and purified water in parts by weight, stirring at room temperature (25°C) and 250 rpm for 12 minutes until completely dissolved, and then set aside.
[0084] The side of the mask substrate that contacts the skin is designated as side a, and the side that does not contact the skin is designated as side b. An enzyme moisturizing composition is coated on side a, and an auxiliary moisturizing composition is coated on side b. The mask substrate is made of cellulose membrane with a thickness of 0.05 mm.
[0085] The specific method for preparing a facial mask is as follows:
[0086] Z1. Cut the cellulose membrane to the size required for facial fitting (21cm long × 15cm wide, with two 2cm diameter eye holes and one 3cm long × 1cm wide opening). Gently wipe the surface of the membrane with purified water to remove dust, and allow it to air dry naturally at room temperature (25℃).
[0087] Z2. Take the enzyme moisturizing composition prepared in Example 2 and apply it evenly to side a of the cellulose membrane at a coating amount of 16 g / m² using a scraper coating machine (coating speed 1.8 m / min, ensuring no bubbles or missed coatings in the coating); then place the membrane material in a constant temperature and humidity chamber, set the temperature to 26℃ and the relative humidity to 40%, and dry for 18 min. Use a rapid moisture meter to detect the moisture content of the functional layer on side a until it reaches 20%, then remove it for later use.
[0088] Z3. Under the same constant temperature and humidity chamber conditions, take the above-mentioned auxiliary moisturizing composition and apply it to the b side of the film material at a coating amount of 10g / m² (the coating direction is perpendicular to the a side to avoid coating overlap); continue drying for 15min, and check the moisture content of the functional layer on the b side until it reaches 21%. Remove and cool to room temperature of 25℃.
[0089] Z4. Cut the dried mask into its final usable size (20cm long × 14cm wide) and package it individually using an aluminum-plastic composite film (inner PE film thickness 45μm, outer aluminum foil thickness 10μm). During packaging, nitrogen gas with a purity ≥99.99% (nitrogen flow rate 0.25L / min) is introduced through a nitrogen replacement device. The oxygen residue in the packaging is detected using a headspace gas analyzer to ensure it is ≤0.6%. After sealing, the mask is sterilized by irradiation with 254nm ultraviolet light for 35s to obtain the finished enzyme moisturizing mask. Store it away from light at a temperature below 25℃.
[0090] Application Example 3
[0091] A face mask includes a face mask substrate, an enzyme moisturizing composition prepared in Example 3, and an auxiliary moisturizing composition.
[0092] The auxiliary moisturizing composition comprises the following components in parts by weight: 3 parts trehalose, 1.0 part sodium hyaluronate, and 8 parts purified water. The preparation method of the auxiliary moisturizing composition is as follows: trehalose, sodium hyaluronate, and purified water are mixed in parts by weight and stirred at room temperature (25°C) and 350 rpm for 8 minutes until completely dissolved.
[0093] The side of the mask substrate that contacts the skin is designated as side a, and the side that does not contact the skin is designated as side b. An enzyme moisturizing composition is coated on side a, and an auxiliary moisturizing composition is coated on side b. The mask substrate is made of cellulose membrane with a thickness of 0.1 mm.
[0094] A method for preparing a face mask is as follows: Z1. Cut the cellulose membrane to the size that fits the face (21cm long × 15cm wide, with two eye holes of 2cm in diameter and one opening of 3cm long × 1cm wide). Gently wipe the surface of the membrane with purified water to remove dust, and let it air dry naturally at room temperature of 25℃.
[0095] Z2. Take the enzyme moisturizing composition prepared in Example 3 and apply it evenly to side a of the cellulose membrane at a coating amount of 20 g / m² using a scraper coating machine (coating speed 2.2 m / min, ensuring no bubbles or missed coatings in the coating); then place the membrane material in a constant temperature and humidity chamber, set the temperature to 30℃ and the relative humidity to 50%, and dry for 12 min. Use a rapid moisture meter to detect the moisture content of the functional layer on side a until it reaches 25%, then remove it for later use.
[0096] Z3. Under the same constant temperature and humidity chamber conditions, take the above-mentioned auxiliary moisturizing composition and apply it to the b side of the film material at a coating amount of 14 g / m² (the coating direction is perpendicular to the a side to avoid coating overlap); continue drying for 10 min, and check the moisture content of the functional layer on the b side until it reaches 24%. Remove and cool to room temperature of 25°C.
[0097] Z4. Cut the dried mask into its final usable size (20cm long × 14cm wide) and package it individually using an aluminum-plastic composite film (inner PE film thickness 55μm, outer aluminum foil thickness 15μm). During packaging, nitrogen gas with a purity ≥99.99% (nitrogen flow rate 0.35L / min) is introduced through a nitrogen replacement device. The oxygen residue in the packaging is detected using a headspace gas analyzer to ensure it is ≤0.4%. After sealing, the mask is sterilized by irradiation with 254nm ultraviolet light for 25s to obtain the finished enzyme moisturizing mask. Store it away from light at a temperature below 25℃.
[0098] Comparative Application Example 1
[0099] The difference from Application Example 1 is that the enzyme moisturizing composition coated in step Z2 is the product prepared in Comparative Example 1.
[0100] Comparative Application Example 2
[0101] The difference from Application Example 1 is that the enzyme moisturizing composition coated in step Z2 is the product prepared in Comparative Example 2.
[0102] Comparative Application Example 3
[0103] The difference from Application Example 1 is that:
[0104] 1. The operation of "distinguishing between side A and side B" is omitted, and only one side of the mask substrate is retained as the coating side;
[0105] 2. Steps Z2 and Z3 are combined as follows: Mix the enzyme moisturizing composition of Example 1 (18g / m² for application) with the auxiliary moisturizing composition of Application Example 1 (12g / m² for application) evenly, and apply it uniformly to one side of the mask substrate with a coating amount of 30g / m² using a scraper coating machine (coating speed 2m / min).
[0106] 3. Then place the membrane material in a constant temperature and humidity chamber (temperature 28℃, relative humidity 45%) for 20 minutes to dry. Use a rapid moisture meter to test the moisture content of the coating layer until it reaches 22%. Remove it and cool it to room temperature.
[0107] Test Example 1
[0108] Oil binding rate test
[0109] Test subjects: the enzyme moisturizing composition prepared in Example 1, the enzyme moisturizing composition prepared in Comparative Example 1, and the enzyme moisturizing composition prepared in Comparative Example 2.
[0110] Test method: Mix palmitic acid, stearic acid and oleic acid in a mass ratio of 3:2:1, add liquid paraffin to prepare a simulated oil solution with a concentration of 0.5 g / mL, and dissolve at a constant temperature of 40°C for later use; take 5 g of the composition of each test object, place it in a 10 mL centrifuge tube, add 1 mL of the above simulated oil solution, and shake in a constant temperature water bath at 37°C for 30 min (shaking rate 150 rpm) to ensure that the oil and composition are in full contact. After shaking, the mixture was centrifuged at 5000 rpm for 10 min. The unbound oil solution in the upper layer was collected, and the mass of the upper oil layer was determined using a gas chromatograph (Agilent 7890A). The chromatographic conditions were as follows: HP-5 capillary column (30 m × 0.32 mm × 0.25 μm); column temperature program: initial temperature 60 °C held for 2 min, increased to 280 °C at a rate of 10 °C / min and held for 5 min; injection port temperature 280 °C; detector (FID) temperature 300 °C; carrier gas nitrogen (purity ≥ 99.999%), flow rate 1.0 mL / min; injection volume 1 μL; split ratio 10:1. The oil mass was calculated using the external standard method based on the peak area, and then the oil binding rate was calculated (oil binding rate = (mass of added oil - mass of unbound oil) / mass of added oil × 100%).
[0111] Test index: Oil binding rate (%) of each group of enzyme moisturizing compositions.
[0112] The data for Test Example 1 are shown in Table 1.
[0113] Table 1
[0114] Test group Oil binding rate Example 1 95.3% Comparative Example 1 12.7% Comparative Example 2 68.5%
[0115] As shown in Table 1, the oil binding rate of Example 1 is significantly higher than that of Comparative Example 1 and Comparative Example 2, indicating that the amphiphilic complex (phosphatidylcholine, PEG-10 stearate and lipase in proportion) in this invention can effectively overcome the oil barrier on the skin surface, and the addition of lipase plays a key role in improving the oil binding efficiency.
[0116] Test Example 2
[0117] Oil migration rate test
[0118] Test subjects: the face mask prepared using Example 1 and the face mask prepared using Example 3 as a comparison.
[0119] Test method: Pigskin with a thickness of 0.5 mm and subcutaneous fat removed was repeatedly washed three times with physiological saline and drained. It was then allowed to stand at 25°C for 30 minutes to equilibrate, and then cut into 5cm×5cm pieces. 0.1g of simulated oil prepared in Test Example 1 was evenly coated on the surface and allowed to stand for 5 minutes. The a-side of the mask used in Application Example 1 and the coated side of the mask used in Comparative Application Example 3 were respectively attached to the oil-coated surface of the pigskin and pressed at a constant temperature of 37°C (pressure 0.02MPa) and allowed to stand for 20 minutes. After removing the masks, the residual substances on the a-side, b-side of Application Example 1 and the coated side of Comparative Application Example 3 were scraped off. The oil was extracted using Soxhlet extraction and the amount of oil adsorbed on each side was calculated by gravimetric method. The oil migration rate was calculated (oil migration rate = oil adsorption amount on the b-side of the experimental group / (oil adsorption amount on the a-side of the experimental group + oil adsorption amount on the b-side of the experimental group) × 100%).
[0120] Test indicators: oil migration rate (%) of the experimental group mask and oil adsorption uniformity of the control group mask (to determine whether there is oil accumulation).
[0121] The data for Test Example 2 are shown in Table 2.
[0122] Table 2
[0123] Test group Oil migration rate (%) Application Example 1 88.7% Comparative Application Example 3 12.3%
[0124] As shown in Table 2, the oil migration rate of Application Example 1 reached 88.7%, which is significantly higher than the 12.3% of the comparative Application Example 3. This proves that the layered design of side a and side b in this invention can effectively realize the directional migration of oil from the skin contact surface (side a) to the non-contact surface (side b), solving the problem that the efficacy of existing enzyme-containing masks is limited due to oil blockage.
[0125] Test Example 3
[0126] Skin moisturizing effect test
[0127] Test subjects: face masks prepared in Application Example 1, Application Example 2, and Application Example 3; and face masks prepared in Comparative Application Example 1, Comparative Application Example 2, and Comparative Application Example 3.
[0128] Test Method: Thirty healthy volunteers (aged 22-35, combination skin, no history of skin diseases, no history of using strong moisturizing or exfoliating skincare products within the past month) were randomly divided into 6 groups (5 people in each group, male-to-female ratio 1:1). 24 hours prior to the test, a 3cm x 3cm test area was marked on the inner forearm of each volunteer; no skincare products were applied to this area. On the day of the test, 0.05g of simulated residual skin oil was applied to the test area using a pipette and left to stand at 25°C for 10 minutes. After each group applied their corresponding face mask for 20 minutes, the mask was removed, and the test area was gently wiped with a sterile tissue. Residual liquid (consistent application to avoid damaging the stratum corneum); the moisture content of the stratum corneum in the affected area was measured using a skin moisture meter (model: Corneometer CM825, CK GmbH, Germany) at four time points: "before use (after oil pretreatment)", "immediately after use", "2 hours after use", and "4 hours after use". The test environment conditions were: temperature 22±2℃, relative humidity 50±5%. The instrument was calibrated before each test. Three test points were randomly selected in the test area at each time point, and the average value was taken as the moisture content data for that time point.
[0129] Test indicators: Skin stratum corneum moisture content (%) and moisture content improvement rate ((moisture content after use - moisture content before use) / moisture content before use × 100%) of each group of masks at different time points.
[0130] The data for Test Example 3 are shown in Table 3.
[0131] Table 3
[0132] Test group Moisture content (%) before use Immediate moisture content after use (%) Moisture gain rate (%) Moisture content (%) 2 hours after use Moisture content (%) 4 hours after use Application Example 1 15.2 38.5 153.3 33.2 28.7 Application Example 2 15.0 36.8 145.3 31.5 27.1 Application Example 3 15.3 37.2 143.8 32.0 27.5 Comparative Application Example 1 15.1 22.8 51.0 19.5 17.2 Comparative Application Example 2 15.4 25.6 66.2 21.3 18.9 Comparative Application Example 3 15.2 24.7 63.2 20.5 18.1
[0133] As shown in Table 3, the mask prepared in Example 1 (Application Example 1) achieved an immediate moisture increase rate of 153.3% after use, the highest among all test groups. Furthermore, it maintained a high moisture content (28.7%) even 4 hours after use, indicating its long-lasting moisturizing effect. In contrast, the moisture increase rates of the control groups were all below 70%, and moisture loss was rapid over time. This demonstrates that the enzyme moisturizing composition of the present invention, along with its layered design of sides A and B, effectively solves the oil barrier problem, allowing the enzyme components to fully exert their moisturizing and barrier repair effects.
[0134] In summary, this invention, through the innovative design of an amphiphilic complex (phosphatidylcholine, PEG-10 stearate, and lipase in a specific ratio) and a layered membrane structure with sides a and b, successfully solves the problem of limited moisturizing effect caused by residual oil on the skin surface in enzyme-containing masks. Experimental data show that the oil binding rate of Example 1 reached 95.3%, significantly higher than Comparative Example 1 (12.7%) and Comparative Example 2 (68.5%), confirming the key role of lipase in the amphiphilic complex; the oil migration rate of Application Example 1 was as high as 88.7%, effectively realizing the directional migration of oil from the skin contact surface (side a) to the non-contact surface (side b), avoiding the formation of a barrier layer on side a. Skin moisturizing tests showed that Application Example 1 achieved a moisture enhancement rate of 153.3%, and the moisturizing effect was long-lasting (moisture content still reached 28.7% 4 hours after use), far superior to the control group (maximum 66.2%). This invention not only allows the active enzyme components in the yeast / rice fermentation product filtrate to fully contact the skin and exert their moisturizing and barrier repair effects, but also significantly improves the lasting moisturizing effect through a multi-layer moisturizing system (synergistic effect of sodium hyaluronate, glycerin, and panthenol).
[0135] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.
Claims
1. A facial mask, characterized in that, The mask comprises a mask substrate, an enzyme moisturizing composition, and an auxiliary moisturizing composition. The side of the mask substrate that contacts the skin is designated as side a, and the side that does not contact the skin is designated as side b. The enzyme moisturizing composition is applied to side a, and the auxiliary moisturizing composition is applied to side b. The preparation steps of the mask are as follows: First, the enzyme moisturizing composition is applied to side a of the mask substrate and dried until the moisture content of the composition on side a is 20-25%. Then, the auxiliary moisturizing composition is applied to side b of the mask substrate and dried until the moisture content of the composition on side b is 20-25%. The components and weight parts of the enzyme moisturizing composition are as follows: 55-65 parts of yeast / rice fermentation product filtrate, 0.8-1.5 parts of amphiphilic complex, 0.5-1.0 parts of sodium hyaluronate, 6-8 parts of glycerin, 1.5-2.5 parts of panthenol, 3-5 parts of propylene glycol, 0.2-0.4 parts of sodium benzoate, and 16.6-33 parts of purified water; the preparation method of the enzyme moisturizing composition is as follows: Y1, mix yeast / rice fermentation product filtrate with purified water, stir at room temperature until uniform to obtain mixture A; Y2, add sodium hyaluronate, glycerin, panthenol and propylene glycol to mixture A in sequence, stir at 25-30℃ until completely dissolved to obtain mixture B; Y3, add amphiphilic complex to mixture B, and simultaneously purge the stirring container with nitrogen for protection, stir evenly, adjust the pH to 5.5-6.0, then add sodium benzoate, stir until uniform, and obtain the enzyme moisturizing composition; The amphiphilic complex is prepared by the following method using phosphatidylcholine, PEG-10 stearate, and lipase in a mass ratio of (0.3-0.5):(0.3-0.5):(0.2-0.3): S1. Phosphatidylcholine and PEG-10 stearate are dissolved in an aqueous ethanol solution to obtain mixture a; S2. The temperature of mixture a is adjusted to 0-4℃, lipase is added, and the mixture is stirred evenly to obtain mixture b; S3. Mixture b is filtered through a microporous membrane and then ultrasonically treated to obtain the amphiphilic complex, wherein the pore size of the filter membrane is 0.3-0.4 μm. The auxiliary moisturizing composition consists of the following components in parts by weight: 2-3 parts trehalose, 0.5-1.0 parts sodium hyaluronate, and 6-8 parts purified water.
2. The facial mask according to claim 1, characterized in that, The mask substrate is a cellulose membrane, and the thickness of the mask substrate is 0.05-0.1 mm.