Biomacromolecule desalination dialysis device

Abstract

A biomacromolecule desalination dialysis device belongs to the field of biomacromolecule desalination and purification and comprises a dialysate box, a detachable dialysis clamping frame arranged in the dialysate box and a dialysis card inserted into a clamping hole of the clamping frame, the dialysis card is provided with a clamping head, the clamping head is provided with a liquid injection port and an air hole, the lower end of the liquid injection port is connected with a dialysis groove, and the lower end of the clamping head is coated with two filter membranes. A dialysis space isolated from the outside is formed and communicates with the dialysis tank. The device further comprises a cover plate and a pressing strip, the dialysis card frame is provided with a fixing groove, and the dialysis cards can be arranged in multiple rows through the connecting grooves and the connecting cards. The membrane interval is 0-17mm, and gt can be reserved; according to the present invention, the kit has a molecular weight of 2,000 Dalton, a part is made of polypropylene, has a specification of 24 or 48 or 96 holes, is compatible with standard equipment, can rapidly dialyze 1-96 parts of 10-5000 [mu] L samples, and can remove gt in 2-4 h; the method is suitable for desalination application of biological macromolecules such as polypeptide, antibody and nucleic acid, and has the advantages of high efficiency, high flux, simplicity and convenience in operation and the like.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological macromolecule desalination purification, and particularly relates to a biological macromolecule desalination dialysis device. BACKGROUND

[0002] With the rapid development of biotechnology, higher requirements are put forward for the efficiency, flux and automation degree of biological macromolecule desalination dialysis.

[0003] In the research and production process of biological macromolecules, desalination and buffer replacement are very important steps. The traditional biological macromolecule desalination dialysis method usually adopts a dialysis bag, and this method has many shortcomings: low dialysis efficiency, usually requiring a long time to complete the desalination process, generally more than 12 hours, or even longer, which seriously affects the experimental progress and production efficiency. Complex operation, the sample needs to be loaded into the dialysis bag, sealed and placed in the dialysis liquid, and the dialysis liquid needs to be replaced during the period, which increases the workload and operation difficulty of the experimental personnel. Low flux, only a small amount of sample can be processed at a time, which cannot meet the needs of high-throughput experiments and production. Low sample recovery rate, due to the adsorption of the dialysis bag and the loss in the operation process, which often leads to high sample loss. Easy to be contaminated, the sample is easy to contact with the external environment during the operation process, which increases the risk of contamination. Not convenient for automatic operation, which cannot be compatible with modern laboratory automation equipment, limiting its application in large-scale production and high-throughput screening.

[0004] Therefore, there is an urgent need for a new biological macromolecule desalination dialysis device which can overcome the above-mentioned shortcomings. SUMMARY

[0005] In view of the above problems, the present application is proposed to provide a biological macromolecule desalination dialysis device which overcomes the above-mentioned problems or at least partially solves the above-mentioned problems, to solve the problems of low dialysis efficiency, complex operation, low flux and low sample recovery rate in the prior art, realize rapid, efficient and high-throughput desalination dialysis of biological macromolecules, and facilitate automatic operation and reduce the risk of contamination.

[0006] In order to solve the above-mentioned problems, the present application discloses a biological macromolecule desalination dialysis device, comprising: a dialysis liquid box, which is internally provided with a dialysis card holder which is detachably arranged; at least one card hole is arranged on the dialysis card holder, and a dialysis card is arranged in the card hole; The dialysis card is provided with a card head, the card head is provided with a liquid injection port and a gas permeation hole, and the lower end of the liquid injection port is connected with a dialysis tank. The lower end of the card head is covered with two filter membranes; the lower end of the card head forms a dialysis space which is isolated from the outside, and the dialysis tank is in communication with the dialysis space.

[0007] Optionally, the dialysis card frame is further provided with a fixing groove, and the inner wall of the dialysis liquid box is provided with a protruding structure matched with the fixing groove, so as to realize detachable connection of the dialysis card frame and the dialysis liquid box.

[0008] Optionally, the dialysis card further comprises a connecting groove and a connecting card, and adjacent dialysis cards are detachably connected into a multi-row dialysis device through the connecting groove and the connecting card.

[0009] Optionally, the interval of the two filter membranes is 0-17 mm.

[0010] Optionally, the filter membrane comprises a dialysis membrane capable of retaining proteins and macromolecules of the same order of magnitude with a molecular weight greater than 2000 daltons, an ultrafiltration membrane, a reverse osmosis membrane, a regenerated cellulose membrane and a PES membrane.

[0011] Optionally, it further comprises a cover plate and a pressing strip, the cover plate is arranged above the dialysis liquid box, and the pressing strip is arranged between the cover plate and the dialysis card frame and used for fixing the dialysis card.

[0012] Optionally, the lower end of the card head of the dialysis card is further provided with a support frame, and the support frame is used for supporting the filter membrane and keeping the interval of the two membranes.

[0013] Optionally, the dialysis liquid box, the dialysis card frame and the dialysis card are all made of high-quality polypropylene.

[0014] Optionally, the specification of the dialysis device is 24 wells or 48 wells or 96 wells, and the dialysis device is compatible with standard 96-well laboratory equipment and automated liquid handling systems.

[0015] Optionally, the volume of the dialysis tank is 10-5000 μL, which can quickly dialyze 1-96 samples, and remove >95% of small molecules within 2-4 hours.

[0016] The present application comprises the following advantages: High dialysis efficiency: short diffusion distance (0-17 mm interval between two membranes) and large surface area, so that the dialysis process can be completed within 2-4 hours, greatly improving the dialysis efficiency, and saving a lot of time compared with the traditional method.

[0017] High throughput: the device is provided with 24 wells or 48 wells or 96 wells, which can simultaneously process 1-96 samples, meet the needs of high-throughput experiments and production, and significantly improve the work efficiency.

[0018] Easy to operate: standard laboratory micropipette is used for sample recovery, without centrifuge or complex operation, which simplifies the experimental process and reduces the operation difficulty.

[0019] High sample recovery rate: The dialysis card is made of a low-binding-force material and has a reasonable structural design, which can effectively reduce sample adsorption and loss, and the protein recovery rate exceeds 90%.

[0020] Flexible and detachable: The dialysis cards can be removed from the plate and disassembled into individual dialysis units. Users can use only the required number of dialysis units according to their actual needs, avoiding waste and improving the utilization rate of the equipment.

[0021] Contamination and evaporation protection: Components such as dialysis fluid boxes, dialysis card holders, and covers, made of high-quality polypropylene, effectively protect samples from contamination and evaporation, ensuring sample stability, especially during long-term storage.

[0022] High compatibility: Compatible with standard 96-well laboratory equipment and automated liquid handling systems, facilitating automated operation and suitable for applications such as large-scale production and high-throughput screening.

[0023] It has a wide range of applications: it can be used in various biomacromolecule-related experiments and production processes, such as protein and DNA desalting / buffer exchange, removal of dyes after protein labeling, enzyme activity / binding assays, and drug binding studies. Attached Figure Description

[0024] Figure 1 This is a schematic diagram of a biomacromolecule desalination dialysis device with a shorter dialysis card provided in an embodiment of the present invention; Figure 2 This is a schematic diagram of a biomacromolecule desalting dialysis device with a relatively long dialysis card provided in an embodiment of the present invention; Figure 3 This is a schematic diagram of the three-dimensional structure of a shorter dialysis card in a biomacromolecule desalination dialysis device provided in an embodiment of the present invention; Figure 4 This is a schematic diagram of the shorter front structure of a biomacromolecule desalination dialysis device provided in an embodiment of the present invention; Figure 5 This is a schematic diagram of a long dialysis card structure of a biomacromolecule desalination dialysis device provided in an embodiment of the present invention; Figure 6 This is a schematic diagram of the front structure of a biomacromolecule desalting dialysis device provided in an embodiment of the present invention; Figure 7 This is a top-view structural diagram of a dialysis card in a biomacromolecule desalination dialysis device according to an embodiment of the present invention; Figure 8 This is a schematic diagram of the dialysis card assembly of a biomacromolecule desalination dialysis device provided in an embodiment of the present invention.

[0025] In the attached diagram, 1 is the dialysate box; 2 is the dialysate card holder; 3 is the dialysate card; 4 is the pressure strip; 5 is the cover plate; 21 is the card hole; 22 is the fixing groove; 31 is the card head; 32 is the connecting groove; 33 is the connecting card; 34 is the dialysate tank; 35 is the injection port; 36 is the vent hole; and 37 is the support frame. Detailed Implementation

[0026] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0027] An embodiment of a biomacromolecule desalting dialysis device provided by the present invention, such as... Figures 1 to 8 As shown, the above-mentioned device includes a dialysate box 1, which has a detachably mounted dialysate card holder 2 inside; the dialysate card holder 2 has at least one card hole 21, into which a dialysate card 3 is inserted; the dialysate card 3 has a card head 31, which has an injection port 35 and a vent 36, and the lower end of the injection port 35 is connected to a dialysate tank 34; the lower end of the card head 31 is covered with two filter membranes, such as one or a combination of two of the following: a dialysate membrane, an ultrafiltration membrane, a reverse osmosis membrane, a regenerated cellulose membrane, or a PES membrane; so that the lower end of the card head 31 forms a dialysate space isolated from the outside, and the dialysate tank 34 is connected to the dialysate space.

[0028] The dialysate container 1, as the main container of the device, is used to hold the dialysate. The lower end of the dialysate card holder 2 is equipped with support feet, which serve two purposes: providing support and guiding and limiting the position of the card holder 2 during placement and removal, preventing tilting or displacement, and allowing for detachable installation within the dialysate container 1. The dialysate container 1 is made of high-quality polypropylene, possessing good chemical stability and anti-fouling capabilities. The dialysate card holder 2, installed within the dialysate container 1, has multiple slots 21 for inserting dialysate cards 3. The number and arrangement of the slots 21 depend on the device specifications (24, 48, or 96 slots). The dialysate card holder 2 also has a fixing groove 22 to accommodate the edge of the card head 31 of the dialysate card 3, ensuring stable installation of rows of dialysate cards 3 within the dialysate container 1. The dialysate card 3 is the core component of the device; each card corresponds to the dialysis treatment of one sample. The dialysis card 3 has a card head 31, which has an injection port 35 and a vent 36. The injection port 35 is used to inject the sample into the dialysis tank 34, and the vent 36 is used to balance the air pressure during the dialysis process, ensuring smooth sample injection and normal dialysis. The lower end of the injection port 35 is connected to the dialysis tank 34, which has a volume of 10–5000 μL, which can be selected according to the sample volume. The lower end of the card head 31 is covered with two dialysis membranes, ultrafiltration membranes, reverse osmosis membranes, regenerated cellulose membranes, or PES membranes, with a spacing of 0–17 mm between the two membranes. They are supported by a support frame 37 to form a dialysis space isolated from the outside. The dialysis tank 34 is connected to this dialysis space.

[0029] In some embodiments of this application, the dialysis card holder 2 is further provided with a fixing groove 22, and the inner wall of the dialysate box 1 is provided with a protruding structure that cooperates with the fixing groove 22, so as to realize the detachable connection between the dialysis card holder 2 and the dialysate box 1. The dialysis card 3 also includes a connecting groove 32 and a connecting clip 33, and adjacent dialysis cards 3 can be detachably connected to form a multi-row (e.g., 4, 6, 8, or 12 rows, etc.) dialysis device through the connecting groove 32 and the connecting clip 33.

[0030] The aforementioned dialysis card 3 also includes a connecting slot 32 and a connecting card 33. Adjacent dialysis cards 3 can be detachably connected to form a single row through the connecting slot 32 and the connecting card 33. The number of dialysis cards 3 in each row is determined by the number of rows of card holes 21 on the card holder 2. For example, if the card holder 2 has 48 card holes 21, and its card holes 21 are counted as 8 rows and 6 columns, then each row is composed of 6 dialysis cards 3. By setting up the same 8 rows, an 8-row dialysis device can be formed, which is convenient for users to assemble and disassemble according to their needs.

[0031] Furthermore, a retaining strip 4 is positioned between the cover plate 5 and the dialysis card holder 2 to secure the dialysis card 3, ensuring its stability during dialysis. The cover plate 5 is positioned above the dialysis fluid container 1, providing a seal and protection to prevent sample contamination and evaporation.

[0032] In some embodiments of this application, the spacing between the two dialysis membranes, ultrafiltration membranes, reverse osmosis membranes, regenerated cellulose membranes, or PES membranes is 0–17 mm. The filtration membranes include dialysis membranes, ultrafiltration membranes, reverse osmosis membranes, regenerated cellulose membranes, and PES membranes, capable of retaining proteins with molecular weights greater than 2000 Daltons and equivalent macromolecules; any one of these can be selected according to requirements. With a membrane spacing of 0–17 mm, it can retain molecules >2000 Daltons. The components are made of polypropylene, with specifications of 24, 48, or 96 wells, compatible with standard equipment. It can rapidly dialyze 1–96 samples of 10–5000 μL, removing >95% of small molecules in 2–4 hours. It is suitable for desalting applications of biomacromolecules such as peptides, antibodies, and nucleic acids, and has advantages such as high efficiency, high throughput, and ease of operation.

[0033] Furthermore, it also includes a cover plate 5 and a pressure strip 4. The cover plate 5 covers the dialysis fluid box 1, and the pressure strip 4 is disposed between the cover plate 5 and the dialysis card holder 2 to fix the dialysis card 3. The lower end of the card head 31 of the dialysis card 3 is also provided with a support frame 37. The support frame 37 is used to support the dialysis membrane, ultrafiltration membrane, reverse osmosis membrane, regenerated cellulose membrane, or PES membrane, so that the two membranes are kept apart.

[0034] The dialysis fluid box 1, dialysis card holder 2, and dialysis card 3 are all made of high-quality polypropylene, which helps protect the sample from contamination and evaporation, especially during long-term storage.

[0035] The dialysis apparatus is available in 24, 48, or 96-well configurations and is compatible with standard 96-well laboratory equipment and automated liquid handling systems. The dialysis tank 34 has a volume of 10–5000 μL, enabling rapid dialysis of 1–96 samples and removal of >95% of small molecules within 2–4 hours.

[0036] When using the biomacromolecule desalting dialysis device of the present invention for biomacromolecule desalting dialysis, the specific operating steps are as follows: Assembly device: Depending on the number of samples to be processed, the dialysis card holder 2 is installed in the dialysis fluid box 1 through the fixing groove 22, and then the dialysis card 3 is inserted into the card hole 21 of the dialysis card holder 2. The dialysis cards 3 can be connected into a multi-row dialysis device or disassembled into a single dialysis device as needed.

[0037] Sample and dialysis fluid injection: Inject the biomacromolecule sample to be desalted into the dialysis tank 34 through the injection port 35, and simultaneously inject an appropriate amount of dialysis fluid into the dialysis fluid container 1. Then, install the pressure strip 4 and the cover plate 5 on the dialysis fluid container 1 to fix the dialysis card 3.

[0038] Dialysis is performed in the dialysis tank 34, where the sample exchanges substances with the dialysate in the dialysate container 1 through two dialysis membranes, ultrafiltration membranes, reverse osmosis membranes, regenerated cellulose membranes, or PES membranes. Due to the selective permeability of the membranes, biomacromolecules with a molecular weight greater than 2000 Daltons are retained in the dialysis space, while impurities such as salts and small molecules diffuse through the membranes into the dialysate, thus achieving desalination of biomacromolecules. Because the distance between the two membranes is only 0–17 mm, the diffusion distance is short, and the surface area is large, the dialysis process can be carried out rapidly, usually completed within 2–4 hours.

[0039] Sample recovery: After dialysis, remove the cover plate 5 and the pressure strip 4, take out the dialysis card 3, and use a standard laboratory micropipette to recover the sample in the dialysis tank 34 through the injection port 35.

[0040] For example, a protein desalting experiment was conducted using a 96-well (8 rows × 12 columns) biomacromolecule desalting dialysis device: Take a 96-well dialysis device, install the dialysis card holder 2 in the dialysis fluid box 1, then select 96 individual dialysis cards 3, connect 12 cards in a row, insert each row of dialysis cards 3 into the card hole 21, and fix the card head 31 of the dialysis cards 3 at both ends to the fixing groove 22 of the dialysis card holder 2. Insert 8 rows in this way.

[0041] Insert 96 dialysis cards 3 into the card slots 21 to form a 96-row dialysis device.

[0042] 200 μL of protein sample containing salt impurities is injected into the dialysis tank 34 of each dialysis card 3 through the injection port 35, while an appropriate amount of dialysis fluid is injected into the dialysis fluid box 1.

[0043] Cover with pressure strip 4 and cover plate 5, place the device in a suitable environment for dialysis, control the temperature at 4℃, and dialysis time for 3 hours.

[0044] After dialysis is complete, remove the cover plate 5 and the pressure strip 4, and use a micropipette to recover the protein sample from each dialysis tank 34.

[0045] The recovered protein samples were tested, and the results showed that the protein recovery rate was over 90% and the salt removal rate was greater than 95%, which met the experimental requirements.

[0046] It should be noted that the above-mentioned dialysis card 3, as shown in Figure 3, is a dialysis card with a Figure 3 and Figure 4 Or such as Figure 5 andFigure 6 As shown, longer or shorter dialysis cards 3 can be used, along with corresponding dialysis card holders 2 and dialysis fluid boxes 1. Their specific lengths can be customized according to usage requirements.

[0047] In some embodiments of this application, a biomacromolecule desalting dialysis device is provided, which allows for desalting dialysis within a single, detachable dialysis unit. From top to bottom, it includes a cover plate 5, a pressure strip 4, a dialysis card 3, a dialysis card holder 2, and a dialysate container 1. This dialysis device consists of two dialysis membranes, ultrafiltration membranes, reverse osmosis membranes, regenerated cellulose membranes, or PES membranes spaced 0–2 mm apart. The shorter diffusion distance and larger surface area facilitate rapid dialysis, typically removing salts and small molecules within 2–4 hours. Sample recovery is performed using a standard laboratory micropipette. The dialysis card skeleton (i.e., the portion of the dialysis card 3 excluding the membrane), the dialysis card holder 2, and the dialysate container 1 are made of high-quality polypropylene, which helps protect samples from contamination and evaporation, especially during long-term storage.

[0048] Each set of this biomacromolecule desalting dialysis device contains 48 or 96 dialysis cards 3, pre-loaded into six or twelve 8-unit dialysis units. Each dialysis card 3 unit can be removed from the plate and disassembled into individual dialysis units, thus using only the required number of units and avoiding waste. The sample chamber within each dialysis card 3 is made of two low-binding regenerated cellulose membranes or PES, which are capable of retaining proteins and other macromolecules >2,000 Daltons while removing buffer salts and contaminants.

[0049] This method enables rapid dialysis of 1 to 96 samples with volumes ranging from 10 to 2000 μL. Using this device, samples can be efficiently dialyzed and desalted in standard 96-well deep-well plates with minimal buffer, while also removing >95% of small molecules. This biomolecule desalting dialysis device is compatible with standard 96-well laboratory equipment and automated liquid handling systems, making it ideal for high-throughput applications. It eliminates the need for centrifuges or complex operations, simplifying the process and avoiding the risk of cross-contamination associated with tube transfer.

[0050] Furthermore, the aforementioned device is preferably 48-well or 96-well, and the biomacromolecule desalting dialysis device can be used as a microdialysis plate. This microdialysis plate is a microplate compatible with automation, used for the rapid dialysis of 1 to 96 samples with volumes of 10 to 1000 μL. Using the microdialysis plate, samples can be dialyzed efficiently with minimal buffer in a standard 96-well deep-well plate, while also removing >95% of small molecules. This dialysis plate is compatible with standard 96-well laboratory equipment and automated liquid handling systems, making it ideal for high-throughput applications.

[0051] Each sample treated with the microdialysis plate is dialyzed within a single, removable dialysis unit consisting of two regenerated cellulose membranes spaced 0–2 mm apart. The short diffusion distance and large surface area facilitate rapid dialysis, typically removing salts and small molecules within 2–4 hours. Sample recovery is performed using a standard laboratory micropipette. The plate sealing membrane is made of high-quality polypropylene, helping to protect the sample from contamination and evaporation, especially during long-term storage.

[0052] The aforementioned biomacromolecule desalting dialysis device features: high yield, resulting in a protein recovery rate exceeding 90%; dialysis can be completed within 2–4 hours, achieving rapid dialysis; it is easy to use, allowing sample loading and retrieval using standard pipette tips; the detachable 8-well unit can be expanded from 1 sample to 96 samples, offering good flexibility; and it is compatible with automation, with the plate design conforming to the SBS microplate standard.

[0053] The aforementioned biomolecular desalting dialysis device comprises twelve pre-assembled octet dialysis units in 48-well or 96-well microdialysis plates. Each dialysis unit can be removed from the plate and assembled into a single microdialysis unit, allowing only the required number of units to be used and avoiding waste. The sample chamber within each microdialysis unit is made of two low-binding regenerated cellulose membranes capable of retaining proteins and other macromolecules >2,000 Daltons while removing buffer salts and contaminants. It can also be used for protein and DNA desalting / buffer exchange, dye removal after protein labeling, enzyme activity / binding assays, and drug binding studies.

[0054] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to each other.

[0055] Although preferred embodiments of the present invention have been described, those skilled in the art, upon learning the basic inventive concept, can make other changes and modifications to these embodiments. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments as well as all changes and modifications falling within the scope of the embodiments of the present invention.

[0056] Finally, it should be noted that in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or terminal device that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or terminal device. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or terminal device that includes said element.

[0057] The above provides a detailed description of the biomacromolecule desalting dialysis device provided by the present invention. Specific examples have been used to illustrate the principle and implementation of the present invention. The description of the above embodiments is only for the purpose of helping to understand the method and core idea of ​​the present invention. At the same time, for those skilled in the art, there will be changes in the specific implementation and application scope based on the idea of ​​the present invention. Therefore, the content of this specification should not be construed as a limitation of the present invention.

Claims

1. A biomacromolecule desalting dialysis device, characterized in that, include: A dialysis fluid cartridge has a detachable dialysis card holder inside; the dialysis card holder has at least one card hole into which a dialysis card is inserted. The dialysis card is equipped with a card head, which has an injection port and a vent hole. The lower end of the injection port is connected to a dialysis tank. The lower end of the dialysis head is covered with two filter membranes, forming a dialysis space isolated from the outside at the lower end of the dialysis head, and the dialysis tank is connected to the dialysis space.

2. The apparatus according to claim 1, characterized in that, The dialysis card holder is also provided with a fixing groove, and the inner wall of the dialysis fluid box is provided with a protruding structure that cooperates with the fixing groove, so as to realize the detachable connection between the dialysis card holder and the dialysis fluid box.

3. The biomacromolecule desalting dialysis device according to claim 1, characterized in that, The dialysis card also includes a connecting slot and a connecting card, and adjacent dialysis cards can be detachably connected to form a multi-row dialysis device through the connecting slot and the connecting card.

4. The biomacromolecule desalting dialysis device according to claim 1, characterized in that, The spacing between the two filter membranes is 0 to 17 mm.

5. The biomacromolecule desalting dialysis device according to claim 4, characterized in that, The filtration membranes include dialysis membranes, ultrafiltration membranes, reverse osmosis membranes, regenerated cellulose membranes, and PES membranes, which are capable of retaining proteins with a molecular weight greater than 2000 Daltons and macromolecules of the same magnitude.

6. The biomacromolecule desalting dialysis device according to claim 1, characterized in that, It also includes a cover plate and a pressure strip. The cover plate is placed over the dialysis fluid box, and the pressure strip is placed between the cover plate and the dialysis card holder to fix the dialysis card.

7. The biomacromolecule desalting dialysis device according to claim 1, characterized in that, The dialysis card is also provided with a support frame at the lower end of the card head. The support frame is used to support the filter membrane and keep the two membranes spaced apart.

8. The biomacromolecule desalting dialysis device according to claim 1, characterized in that, The dialysis fluid box, dialysis card holder, and dialysis card are all made of high-quality polypropylene.

9. The biomacromolecule desalting dialysis device according to claim 1, characterized in that, The dialysis device is available in 24, 48, or 96 well configurations and is compatible with standard 96-well laboratory equipment and automated liquid handling systems.

10. The biomacromolecule desalting dialysis device according to claim 1, characterized in that, The dialysis cell has a volume of 10–5000 μL and can rapidly dialyze 1–96 samples, removing >95% of small molecules within 2–4 hours.

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